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exploring methionine γ-lyase structure-function relationship via microspectrophotometry and x-ray crystallography.pyridoxal 5'-phosphate (plp) dependent methionine γ-lyase catalyzes the breakdown of l-methionine to α-ketobutyric acid, methanethiol and ammonia. this enzyme, present in anaerobic microorganisms, has biomedical interest both for its activity as antitumor agent, depleting methionine supply in methionine-dependent cancers, and as target in the treatment of human pathogen infections, activating the pro-drug trifluoromethionine. to validate the structure of the enzyme from citrobacter freundii, cry ...201120601224
espj effector in enterohemorrhagic e. coli translocates into host mitochondria via an atypical mitochondrial targeting signal.ehec is a bacterial pathogen causing diarrhea and hemorrhagic colitis in humans. to exert virulence, ehec exploits a subset of effectors that are translocated into host cells via the type iii secretion system. espj, which was recently identified as a type iii secreted effector, is conserved in related pathogens such as epec and citrobacter rodentium. however, the exact function of espj remains unclear. in the present study, we found that espj was unstable in host cells, which might be attributab ...201020618683
production of antimicrobial silver nanoparticles in water extracts of the fungus amylomyces rouxii strain ksu-09.a fungal strain, ksu-09, isolated from the roots of date palm (phoenix dactylifera), was identified as amylomyces rouxii based on sequence analysis of the internal transcribed spacer (its) region of its rrna genes. mycelia-free water extracts obtained from mycelium suspended in water for 72h facilitated the production of stable, predominantly monodispersed and spherical silver nanoparticles (agnps) in the size range of 5-27nm upon addition of 1mm silver nitrate, as determined by the xrd, afm and ...201020619641
survival of campylobacter jejuni under conditions of atmospheric oxygen tension with the support of pseudomonas spp.campylobacter jejuni is a major food-borne pathogen. despite causing enteritis in humans, it is a well-adapted intestinal microorganism in animals, hardly ever generating disease symptoms. nevertheless, as a true microaerophilic microorganism it is still puzzling how campylobacter cells can survive on chicken meat, the main source of human infection. in this study, we demonstrate that c. jejuni is able to withstand conditions of atmospheric oxygen tension when cocultured with pseudomonas species ...201020639377
osteopontin mediates citrobacter rodentium-induced colonic epithelial cell hyperplasia and attaching-effacing lesions.although osteopontin (opn) is up-regulated in inflammatory bowel diseases, its role in disease pathogenesis remains controversial. the objective of this study was to determine the role of opn in host responses to a non-invasive bacterial pathogen, citrobacter rodentium, which serves as a murine infectious model of colitis. opn gene knockout and wild-type mice were infected orogastrically with either c. rodentium or luria-bertani (lb) broth. mouse-derived opn(+/+) and opn(-/-) fibroblasts were in ...201020651246
pyomyoma in a premenopausal woman with fever of unknown origin.pyomyoma (suppurative leiomyoma) is a rare disease that is a serious complication. most cases have occurred in pregnant or postmenopausal women.201020664442
production of biogenic amines "in vitro" in relation to the growth phase by enterobacteriaceae species isolated from traditional sausages.histidine, lysine, ornithine and tyrosine decarboxylase activities were tested in 79 strains of enterobacteriaceae (41 of hafnia alvei, 17 of serratia liquefaciens, 5 of enterobacter cloacae, 4 of citrobacter braakii, 2 of proteus vulgaris, 2 of proteus mirabilis, 2 of providencia stuartii, 2 of klebsiella terrigena, 1 of rahnella aquatilis, 1 of salmonella arizonae, 1 of citrobacter youngae and 1 of escherichia coli) isolated from botillo, a spanish traditional sausage. in general, the strains ...201020673614
evidence for a bimodal distribution of escherichia coli doubling times below a threshold initial cell concentration.in the process of developing a microplate-based growth assay, we discovered that our test organism, a native e. coli isolate, displayed very uniform doubling times (tau) only up to a certain threshold cell density. below this cell concentration (<or= 100 -1,000 cfu ml-1 ; <or= 27-270 cfu well-1) we observed an obvious increase in the tau scatter.201020678197
qnrb9 in association with tem-116 extended-spectrum beta-lactamase in citrobacter freundii isolated from sewage effluent: first report from italy.the aim of the present study was to investigate the mechanism of quinolone and beta-lactam resistance in an isolate of citrobacter freundii pc2/08 collected from sewage effluent from l'aquila, italy. qnrb-9 and bla(tem-116 )were co-expressed in a large plasmid identified in c. freundi pc2/08 strain. compared to tem-1, tem-116 showed two single mutations: v84i and a184v. the plpc2/08 plasmid conferred resistance to several beta-lactams and fluoroquinolones. tazobactam could be considered a good i ...201020685627
performance of a sulfidogenic bioreactor and bacterial community shifts under different alkalinity levels.the performance of a sulfidogenic bioreactor and the response of bacterial populations to influent alkalinity changes were investigated. the bioreactor reached 40% of sulfate removal efficiency (sre) with 0 mg l(-1) of alkalinity, and single-stranded conformation polymorphism profiles showed that some members of bacteroides, dysgonomonas, sporobacter, quinella, and citrobacter became dominant populations. 16s rrna gene library analysis indicated that the actinobacteria group increased from 0% in ...201020688512
bacteraemia caused by non-freundii, non-koseri citrobacter species in taiwan.this study analysed the clinical characteristics of bacteraemia due to unusual citrobacter species. all non-freundii and non-koseri citrobacter isolates were identified to species level by two commercial identification methods and 16s rrna gene sequence analysis. a total of 306 patients with citrobacter spp. bacteraemia were identified from january 2000 through december 2009. four patients (1.3%) had c. braakii bacteraemia, and one had c. amalonaticus and c. sedlakii sepsis, respectively. miside ...201020708303
novel changes in nf-{kappa}b activity during progression and regression phases of hyperplasia: role of mek, erk, and p38.utilizing the citrobacter rodentium-induced transmissible murine colonic hyperplasia (tmch) model, we measured hyperplasia and nf-κb activation during progression (days 6 and 12 post-infection) and regression (days 20-34 post-infection) phases of tmch. nf-κb activity increased at progression in conjunction with bacterial attachment and translocation to the colonic crypts and decreased 40% by day 20. nf-κb activity at days 27 and 34, however, remained 2-3-fold higher than uninfected control. expr ...201020710027
faecal carriage of extended-spectrum β-lactamase-producing and ampc β-lactamase-producing bacteria among danish army recruits.during may and june 2008, 84 danish army recruits were tested for faecal carriage of extended-spectrum β-lactamase (esbl)-producing and ampc β-lactamase-producing bacteria. three esbl-producing (ctx-m-14a) escherichia coli isolates, two ampc-producing (cmy-2) e. coli isolates and one ampc-producing (cmy-34) citrobacter freundii isolate were detected. two of the ctx-m-14a e. coli isolates had similar pulsed-field gel electrophoresis and multilocus sequence typing profiles, indicating the same ori ...201120718802
modulation of hepatic cytochrome p450s by citrobacter rodentium infection in interleukin-6- and interferon-{gamma}-null mice.after infection with citrobacter rodentium, murine hepatic cytochrome p450 (p450) mrnas are selectively regulated. several serum proinflammatory cytokines are elevated, the most abundant being interleukin-6 (il6). to elucidate the role of cytokines in the regulation of p450s during infection, we orally infected wild-type, il6(-/-), or interferon-γ(-/-) [ifnγ(-/-)] female c57bl/6j mice with c. rodentium and analyzed hepatic p450 expression 7 days later. the majority of p450 mrnas were equally aff ...201020719939
chemical composition and antibacterial activity of essential oils of tripleurospermum disciforme in three developmental stages.tripleurospermum disciforme (c.a. mey) schultz bip. (asteraceae) is a widespread biennial species which also has traditional medicinal uses. according to the few recent studies, essential oils of this species exhibit anti-inflammatory, antispasmodic, antiseptic, antifungal, antiulcer, and antioxidant activity. objective: the chemical compositions of the hydrodistilled oils of t. disciforme of iranian origin are studied in the stages of prior to flowering, flowering, and post flowering, for the f ...201020795784
nosocomial diarrhoea in relation to sanitation state: a study in tikrit, iraq.a cross-sectional hospital-based study of 259 children aged < 5 years was carried out in tikrit, iraq, to identify the prevalence of nosocomial diarrhoea and sources of contamination in the ward environment. nosocomial diarrhoea was diagnosed in 84 children (32.4%). children with diarrhoea were more likely than unaffected children to be bottle-fed, given unboiled water, to have unclean food containers and contaminated bed sheets. three out of 5 brands of formula milk (unopened cans) were contami ...201020799556
sensitization of microcin j25-resistant strains by a membrane-permeabilizing peptide.microcin j25 (mccj25) is a plasmid-encoded, 21-amino-acid, antibacterial peptide produced by escherichia coli. mccj25 inhibits rna polymerase and the membrane respiratory chain. mccj25 uptake into e. coli-sensitive strains is mediated by the outer membrane receptor fhua and the inner membrane proteins tonb, exbb, exbd, and sbma. this peptide is active on some e. coli, salmonella, and shigella species strains, while other gram-negative bacteria, such as clinical isolates of enterobacter cloacae, ...201020802073
polymicrobial necrotizing fasciitis involving enterobacteria producing ctx-m-15 extended-spectrum β-lactamases.necrotizing fasciitis due to multiple gram-negative organisms in a nigerian patient is described. morganella morganii and citrobacter freundii carrying the ctx-m-15 extended-spectrum β-lactamase gene were isolated, highlighting the emergence of this β-lactamase in western africa and its successful spread amongst a wider range of members of the enterobacteriaceae.201120813849
normal organ weights, serum chemistry, hematology, and cecal and nasopharyngeal bacterial cultures in the gray short-tailed opossum (monodelphis domestica).gray short-tailed opossums (monodelphis domestica) currently are used in genetic, developmental, oncology, and neurologic research. little is known about their natural flora or potential for pathogenic infectious disease. the present study aims to improve existing comparative normal blood and organ weight values available to researchers and to describe flora of clinically normal m. domestica to obtain an understanding of potential pathogenic flora in clinically abnormal animals. for evaluation o ...201020819383
[implication of extended-spectrum beta-lactamase enterobacteriaceae in nosocomial infections in neonates].the objective of this study was to determine the incidence of extended-spectrum beta-lactamase (esbls) enterobacteriaceae colonization and infection in hospitalized children.201020826324
phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal.the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ...201020833430
antibiotic susceptibility pattern in urinary isolates of gram negative bacilli with special reference to ampc β-lactamase in a tertiary care hospital.resistance to higher antimicrobial agent is commonly seen in gram negative bacilli. this issue is a challenging problem to the medical practitioners in addition to it is financial impact on the health care system.201020842250
oxygen activation by p450(cin): protein and substrate mutagenesis.a conserved threonine found in the majority of cytochromes p450 (p450s) has been implicated in the activation of dioxygen during the catalytic cycle. p450(cin) (cyp176a) has been found to be an exception to this paradigm, where the conserved threonine has been replaced with an asparagine. prior studies with a p450(cin) n242a mutant established that the asn-242 was not a functional replacement for the conserved threonine but was essential for the regio- and stereocontrol of the oxidation of cineo ...201120851096
detection of kpc-2-producing citrobacter freundii isolates in spain. 201020851814
prevalence of bacteria and parasites in white ibis in egypt.a field survey was conducted to evaluate the prevalence of bacterial infections among free-living white ibis (nipponia nippon) in which 92 bacterial isolates were recovered from 193 different internal organs of 55 apparently healthy ibis. escherichia coli and salmonella spp. were isolated at rates of 43.6% and 14.5%, respectively. the other bacterial pathogens isolated were shigella spp. (34.5%), enterobacter spp. (21.8%) citrobacter spp. (18.1%), klebsiella pneumonia (16.3), staphylococcus aure ...201020857378
isolation and characterization of bacteria from the gut of bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion.bombyx mori l. (lepidoptera: bombycidae) have been domesticated and widely used for silk production. it feeds on mulberry leaves. mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. eleven isolates were obtained from the digestive tract of b. mori, including the gram positive bacillus circulans and gram negative proteus vulgaris, klebsiella pneumoniae, escherichia coli, citro ...201020874394
resistance phenotype-genotype correlation and molecular epidemiology of citrobacter, enterobacter, proteus, providencia, salmonella and serratia that carry extended-spectrum β-lactamases with or without plasmid-mediated ampc β-lactamase genes in thailand.extended-spectrum β-lactamases (esbls) and plasmid-mediated ampc β-lactamases (pampcs) have been increasingly reported among less commonly encountered genera of enterobacteriaceae. however, little is known regarding the genetic characteristics of resistance genes and epidemiology of these genera. lack of accurate esbl and pampc detection may adversely affect therapeutic outcomes. this study investigated resistance phenotype-genotype correlation and molecular epidemiology among six genera of ente ...201020880563
alimentary tract bacteria isolated and identified with api-20e and molecular cloning techniques from australian tropical fruit flies, bactrocera cacuminata and b. tryoni.bacteria were isolated from the crop and midgut of field collected bactrocera cacuminata (hering) and bactrocera tryoni (froggatt) (diptera: tephritidae). two methods were used, firstly isolation onto two types of bacteriological culture media (pyea and tsa) and identification using the api-20e diagnostic kit, and secondly, analysis of samples using the 16s rrna gene molecular diagnostic method. using the api-20e method, 10 genera and 17 species of bacteria in the family enterobacteriaceae were ...201020883132
a bacterial study of pyoderma in belgaum.one hundred children with primary pyoderma and 50 healthy children were included in the study. the swabs collected from the lesions were cultured on various media. the colonies were identified by conventional methods. commonest isolate was staph. aureus (45%), followed by strep. pyogens (35%), e. coli (5%), citrobacter (1%) and staph. and strept. together (14%). staphylococci showed highest resistance to ampicillin (85%), followed by penicillin (78%), tetracycline (40%) and ciprofloxacin (15%). ...199920885049
citrobacter koseri meningitis: another freediving risk?we present a rare case of meningitis caused by citrobacter koseri in an immunocompetent adult who had recently been freediving. middle ear pressure changes from this recreational activity, and the subsequent inflammatory response, are likely to have provided this environmental organism access to the central nervous system, and thus the ability to cause clinically significant infection.201020933000
structure of the o-polysaccharide of citrobacter youngae pcm 1503.the o-polysaccharide (o-antigen) was obtained from the lipopolysaccharide of citrobacter youngae pcm 1503, which is currently classified to the o7 serogroup. the following structure of the tetrasaccharide repeating unit of the polysaccharide was established by sugar and methylation analyses along with 1d and 2d (1)h and (13)c nmr spectroscopy: structural and serological data of the o-antigen suggest that strain pcm 1503 should be reclassified to a new citrobacter serogroup.201020934177
citrobacter freundii-induced cold agglutinin hemolysis. 201020938661
inhibitory effects of lactic acid and lauricidin on spoilage organisms of chicken breast during storage at chilled temperature.different concentrations of lauricidin (lu, containing 1% lactic acid) and lactic acid alone (la) were evaluated for their effectiveness in reducing naturally occurring microflora of raw chicken breasts. chicken breasts were dipped in 0 (control), 0.5, 1.0, 1.5, and 2.0% solutions of lu (w/v) or la (v/v) for 10, 20, and 30 min and stored at 4°c for 14 d. total plate counts (tpc) and populations of pseudomonas spp. and enterobacteriaceae were determined before and after dipping and after storing ...201020947197
responding to infection and apoptosis--a task for th17 cells.two of the critical cytokines required for the differentiation of t helper 17 (t(h)17) cells from naive cd4 t cells are transforming growth factor-beta (tgf-β) and interleukin-6 (il-6). innate recognition of apoptotic cells in the presence of toll-like receptor engagement directs the simultaneous synthesis of these cytokines by antigen-presenting cells (apcs), and as such provides a cytokine milieu that favors t(h)17 cell induction. in this situation, apcs are activated in response to ligands de ...201020958317
citrobacter rodentium infection induces myd88-dependent formation of ubiquitinated protein aggregates in the intestinal epithelium.citrobacter rodentium utilizes a type 3 secretion system (t3ss) to inject effector proteins into host intestinal epithelial cells, causing structural and functional changes in these cells during infection. here, we examined the effects of c. rodentium infection on host cell protein ubiquitination in vivo. we observed the appearance of ubiquitinated protein (ub(+)) aggregates in intestinal epithelial cells near the site of bacterial attachment. formation of aggregates was dependent on t3ss activi ...201020962508
bacterial infection causes stress-induced memory dysfunction in mice.the brain-gut axis is a key regulator of normal intestinal physiology; for example, psychological stress is linked to altered gut barrier function, development of food allergies and changes in behaviour. whether intestinal events, such as enteric bacterial infections and bacterial colonisation, exert a reciprocal effect on stress-associated behaviour is not well established.201020966022
extremely drug-resistant citrobacter freundii isolate producing ndm-1 and other carbapenemases identified in a patient returning from india. 201020974865
occurrence of gram-negative bacteria in hens' eggs depending on their source and storage conditions.the aim of this study was to analyse the qualitative composition of gram-negative microbes, mainly of the family enterobacteriaceae, including pathogenic bacteria such as salmonella, in the albumens and yolks and on the shells of hens' eggs, depending on their source and on the temperature and duration of their storage. a total of 375 table eggs were studied, from a large-scale poultry farm, a small-scale poultry farm and a supermarket. each group was divided into 5 subgroups according to the te ...201021033566
surveillance of antimicrobial susceptibility of aerobic and facultative gram-negative bacilli isolated from patients with intra-abdominal infections in china: the 2002-2009 study for monitoring antimicrobial resistance trends (smart).the objective of this study was to investigate the distribution and susceptibility of aerobic and facultative gram-negative bacilli (gnb) isolated from patients with intra-abdominal infections (iais) in china. from 2002 to 2009, minimum inhibitory concentrations of 14 antibiotics for 3420 aerobic and facultative gnb from up to eight hospitals in six cities were determined by the broth microdilution method. enterobacteriaceae comprised 82.9% (2834/3420) of the total isolates, with escherichia col ...201021036547
investigation of the cytotoxic capacity of some adherent opportunistic enterobacterial strains by the mtt assay and transmission electron microscopy.the purpose of this study was to determine the cytotoxic effect on caco-2 intestinal cells of dialysates obtained from bacterial cultures of some enterobacterial opportunistic strains with different sources of isolation (food, stool culture, acute diarrhoea, urine culture), previously tested and selected for their intensive adherence and invasion capacity to the cellular substratum and also for their cytotoxic effect on cell monolayers. in this study the level of cytotoxicity was measured quanti ...201021053782
bacterial decolorization of black liquor in axenic and mixed condition and characterization of metabolites.the pulping byproducts (black liquor) cause serious environmental problem due to its high pollution load. in order to search the degradability of black liquor, the potential bacterial strains citrobacter freundii (fj581026) and citrobacter sp. (fj581023) were applied in axenic and mixed condition. results revealed that the mixed bacterial culture are more effective than axenic condition and can reduce 82% cod, 79% aox, 79% color and 60% lignin after 144 h of incubation period. additionally, the ...201021061144
salkowski's reagent test as a primary screening index for functionalities of rhizobacteria isolated from wild dipterocarp saplings growing naturally on medium-strongly acidic tropical peat soil.rhizobacteria isolated from wild dipterocarp saplings in central kalimantan, indonesia, were subjected to salkowski's reagent test, which is often used in detecting indolic substances. among 69 isolates grown in a low-nitrogen medium supplemented with l-tryptophan (trp), culture fluids of 29 strains were positive to the test, in which 17 bacteria turned red and other 10 pink. all the red type rhizobacteria actively converted trp into tryptophol (tol), while some yielded indole-3-acetic acid (iaa ...201021071871
companion animals: a relevant source of extended-spectrum β-lactamase-producing fluoroquinolone-resistant citrobacter freundii. 201021074371
rmtd2, a new allele of a 16s rrna methylase gene, has been present in enterobacteriaceae isolates from argentina for more than a decade.the first allele of a 16s rrna methyltransferase gene, rmtd2, conferring very high resistance to all clinically available aminoglycosides, was detected in 7/1,064 enterobacteria collected in 2007. rmtd2 was located on a conjugative plasmid in a tn2670-like element inside a structure similar to that of rmtd1 but probably having an independent assembly. rmtd2 has been found since 1996 to 1998 mainly in enterobacter and citrobacter isolates, suggesting a possible reservoir in these genera. this pre ...201021078935
characterisation of pdus, the pdu metabolosome corrin reductase, and evidence of substructural organisation within the bacterial microcompartment.pdus is a corrin reductase and is required for the reactivation of the cobalamin-dependent diol dehydratase. it is one component encoded within the large propanediol utilisation (pdu) operon, which is responsible for the catabolism of 1,2-propanediol within a self-assembled proteinaceous bacterial microcompartment. the enzyme is responsible for the reactivation of the cobalamin coenzyme required by the diol dehydratase. the gene for the cobalamin reductase from citrobacter freundii (pdus) has be ...201021103360
tumor suppressor foxo3 mediates signals from the egf receptor to regulate proliferation of colonic cells.epithelial proliferation, critical for homeostasis, healing, and colon cancer progression, is in part controlled by epidermal growth factor receptor (egfr). proliferation of colonic epithelia can be induced by citrobacter rodentium infection, and we have demonstrated that activity of tumor suppressor foxo3 was attenuated after this infection. thus the aim of this study was to determine the contribution of foxo3 in egfr-dependent proliferation of intestinal epithelia and colon cancer cell lines. ...201021109589
predation of human pathogens by the predatory bacteria micavibrio aeruginosavorus and bdellovibrio bacteriovorus.the focus of this study was to evaluate the potential use of the predatory bacteria bdellovibrio bacteriovorus and micavibrio aeruginosavorus to control the pathogens associated with human infection.201021114596
vi-crm 197 as a new conjugate vaccine against salmonella typhi.an efficacious, low cost vaccine against typhoid fever, especially for young children, would make a major impact on disease burden in developing countries. the virulence capsular polysaccharide of salmonella typhi (vi) coupled to recombinant mutant pseudomonas aeruginosa exoprotein a (vi-repa) has been shown to be highly efficacious. we investigated the use of carrier proteins included in infant vaccines, standardized the conjugation process and developed key assays required for routine lot rele ...201021115057
the apolipoprotein e-mimetic peptide cog112 inhibits nf-kappab signaling, proinflammatory cytokine expression, and disease activity in murine models of colitis.inflammatory bowel disease (ibd), consisting of crohn's disease and ulcerative colitis, is a source of substantial morbidity and remains difficult to treat. new strategies for beneficial anti-inflammatory therapies would be highly desirable. apolipoprotein (apo) e has immunomodulatory effects and synthetically derived apoe-mimetic peptides are beneficial in models of sepsis and neuroinflammation. we have reported that the antennapedia-linked apoe-mimetic peptide cog112 inhibits the inflammatory ...201021115487
laboratory detection of extended-spectrum-beta-lactamase-producing enterobacteriaceae: evaluation of two screening agar plates and two confirmation techniques.the worldwide prevalence of extended-spectrum-beta-lactamase-producing esbl-producing enterobacteriaceae (esbl-e) is increasing, making the need for optimized detection techniques more urgent. in this study we investigated the performance of two esbl-e screening and two esbl-e confirmation techniques. in accordance with the dutch national guidelines (www.wip.nl), a collection of 642 highly resistant enterobacteriaceae strains, as identified by vitek2, was used to test the performances of two scr ...201021123527
effect of transgenic rhizobacteria overexpressing citrobacter braakii appa on phytate-p availability to mung bean plants.rhizosphere microorganisms possessing phytase activity are considered important for rendering phytate-p available to plants. in the present study, citrobacter braakii phytase gene (appa) was over-expressed in rhizobacteria possessing plant growth promoting (pgp) traits for increasing their potential as bioinoculants. appa was cloned under the lac promoter in the broad host-range expression vector pbbr1mcs2. transformation of the recombinant construct pcbappa resulted in high constitutive phytase ...201021124052
recurrent lower limb abscesses as the primary manifestation of aorto-femoral graft paraprosthetic fistula.a 62-year-old man was presented to our facility with recurrent right lower limb abscesses. he had an aortobifemoral graft for an aortoiliac occlusive disease. the diagnosis of paraprosthetic fistula was confirmed by performing a high-resolution contrast-enhanced computed tomography, whereas labeled leukocyte imaging provided a negative result. the graft present in the right limb was removed and extra-anatomical femoro-femoral bypass was performed along with segmental ileal resection of the affec ...201021126852
a small-molecule inhibitor of the bacterial type iii secretion system protects against in vivo infection with citrobacter rodentium.the type iii secretion system (t3ss) is highly conserved in many gram-negative pathogenic bacteria and functions as an injector of bacterial proteins (effectors) into host cells. t3sss are involved in establishing disease processes, but this machinery is not essential for bacterial growth or homeostasis. thus, t3ss is expected to be a candidate therapeutic target, and inhibitors of t3sss could potentially reduce virulence without causing bacterial death, thereby avoiding any subsequent developme ...201021139624
novel genetic environment of the plasmid-mediated kpc-3 gene detected in escherichia coli and citrobacter freundii isolates from china.the imipenem and meropenem-resistant strains citrobacter freundii hs70 and escherichia coli hs510 were isolated from patients in shanghai, china. by isoelectric focusing, pcr amplification and sequencing, these strains were each found to produce four β-lactamases: tem-1, kpc-3, shv-7 and ctx-m-14. a conjugation experiment and plasmid restriction digestion revealed that the bla (kpc-3) gene was located on the same plasmid in both isolates. bidirectional primer walking sequencing showed that the n ...201021153909
kinetic and spectral parameters of interaction of citrobacter freundii methionine γ-lyase with amino acids.kinetic parameters of citrobacter freundii methionine γ-lyase were determined with substrates in γ-elimination reactions as well as the inhibition of the enzyme in the γ-elimination of l-methionine by amino acids with different structure. the data indicate an important contribution of the sulfur atom and methylene groups to the efficiency of binding of substrates and inhibitors. the rate constants of the enzyme-catalyzed exchange of c-α- and c-β-protons with deuterium were determined, as well as ...201021166645
structures of the o-polysaccharides of salmonella enterica o59 and escherichia coli o15.the o-polysaccharide of salmonella enterica o59 was studied using sugar analysis and 2d (1)h and (13)c nmr spectroscopy, and the following structure of the tetrasaccharide repeating unit was established: →2)-β-d-galp-(1→3)-α-d-glcpnac-(1→4)-α-l-rhap-(1→3)-β-d-glcpnac-(1→ accordingly, the o-antigen gene cluster of s. enterica o59 includes all genes necessary for the synthesis of this o-polysaccharide. earlier, another structure has been reported for the o-polysaccharide of salmonella arizonae (s. ...201021167479
citrobacter koseri as a cause of early periprosthetic infection after primary total hip arthroplasty.periprosthetic joint infection in the acute setting is usually caused by gram-positive species and remains a major problem facing total joint surgeons. we report a case of a 53-year-old male who presented with drainage 3 weeks after primary total hip arthroplasty. citrobacter koseri was cultured from an infected hematoma in his deep tissues. surgical treatment included irrigation and debridement with femoral head and liner exchange. he received a 6-week course of ertapenem and is currently asymp ...201021167674
thoracic empyema after laparoscopic cholecystectomy: an unusual cause.thoracic empyema after laparoscopic cholecystectomy is a rare complication. it is associated with dropped gallstones during the operation. in this case, we report a hepatocellular adenoma hemorrhage underneath an old diaphragm rupture, causing empyema after laparoscopic cholecystectomy.201021170636
bioremediation of phenol by alkaliphilic bacteria isolated from alkaline lake of lonar, india.phenol is an industrially important compound which has a wide range of applications. being highly soluble in water, it appears as the major pollutant in waste waters arising from both phenol manufacturing and from industrial units that utilise phenol. because of its toxicity, bioremediation of phenol is necessary. since some of the phenol-bearing industrial waste waters are alkaline in nature, use of alkaliphilic bacteria for bioremediation of phenol was investigated. alkaliphilic bacteria were ...199821182701
comparative study of biological hydrogen production by pure strains and consortia of facultative and strict anaerobic bacteria.in this paper, a simple and rapid method was developed in order to assess in comparative tests the production of binary biogas mixtures containing co(2) and another gaseous compound such as hydrogen or methane. this method was validated and experimented for the characterisation of the biochemical hydrogen potential of different pure strains and mixed cultures of hydrogen-producing bacteria (hpb) growing on glucose. the experimental results compared the hydrogen production yield of 19 different p ...201021185171
enteropathogenic and enterohemorrhagic escherichia coli type iii secretion effector espv induces radical morphological changes in eukaryotic cells.enteropathogenic escherichia coli (epec) and enterohemorrhagic escherichia coli (ehec) are important human pathogens that rely on translocation of type iii secretion system (t3ss) effectors for subversion of signal transduction pathways and colonization of the mammalian gut mucosa. while a core set of effectors is conserved between epec and ehec strains, a growing number of accessory effectors that were found at various frequencies in clinical and environmental isolates have been recently identi ...201021189318
strain-resolved community genomic analysis of gut microbial colonization in a premature infant.the intestinal microbiome is a critical determinant of human health. alterations in its composition have been correlated with chronic disorders, such as obesity and inflammatory bowel disease in adults, and may be associated with neonatal necrotizing enterocolitis in premature infants. increasing evidence suggests that strain-level genomic variation may underpin distinct ecological trajectories within mixed populations, yet there have been few strain-resolved analyses of genotype-phenotype conne ...201021191099
cd4(+) lymphoid tissue-inducer cells promote innate immunity in the gut.fetal cd4(+) lymphoid tissue inducer (lti) cells play a critical role in the development of lymphoid tissues. recent studies identified that lti cells persist in adults and are related to a heterogeneous population of innate lymphoid cells that have been implicated in inflammatory responses. however, whether lti cells contribute to protective immunity remains poorly defined. we demonstrate that after infection with citrobacter rodentium, cd4(+) lti cells were a dominant source of interleukin-22 ...201021194981
molecular epidemiological survey of citrobacter freundii misidentified as cronobacter spp. (enterobacter sakazakii) and enterobacter hormaechei isolated from powdered infant milk formula.a total of 75 powdered infant milk formula (pif) samples collected from pharmacies and drugstores in western sicily, italy, and representative of 12 different brands were analyzed in this study to evaluate their microbiological quality. according to the u.s. food and drug administration protocol, 32 samples out of 75 were contaminated by enterobacteria. commercial biochemical api(r) 20e-system identification method indicated that six pif samples were presumptively contaminated by cronobacter spp ...201121204699
development of a genetic transfer system in selenate-respiring bacterium citrobacter sp. strain jsa which was isolated from natural freshwater sediment.conjugative mating between the selenate-reducing bacterium citrobacter sp. strain jsa and escherichia coli s17-1 harboring the broad-host-range plasmid pkt230 or pkt240 (incq) allowed genetic transfer to strain jsa at a maximum frequency of 2.5×10(-5) (pkt230) and 5.1×10(-6) (pkt240) per recipient jsa cell. kanamycin-resistant (selection marker of pkt230 and pkt240) transconjugants were routinely obtained with this method, and we confirmed that both vectors were also successfully transferred and ...201121215694
characterization of the enterobacteriaceae community that developed during storage of minced beef under aerobic or modified atmosphere packaging conditions.the whole cell protein and macrorestriction analysis of dna of enterobacteriaceae isolates recovered from minced beef stored at 0, 5, 10 and 15 °c aerobically and under modified atmosphere packaging consisting of 40% co(2)-30% o(2)-30% n(2) in the presence (map+) and absence (map-) of oregano essential oil were studied. sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) profiles obtained from whole cell protein analysis of the enterobacteriaceae isolates revealed seven groups. ...201021216482
arsenite-oxidizing and arsenate-reducing bacteria associated with arsenic-rich groundwater in taiwan.drinking highly arsenic-contaminated groundwater is a likely cause of blackfoot disease in taiwan, but microorganisms that potentially control arsenic mobility in the subsurface remain unstudied. the objective of this study was to investigate the relevant arsenite-oxidizing and arsenate-reducing microbial community that exists in highly arsenic-contaminated groundwater in taiwan. we cultured and identified arsenic-transforming bacteria, analyzed arsenic resistance and transformation, and determi ...201021216490
antibiotic resistant bacteria as bio-indicator of polluted effluent in the green turtles, chelonia mydas in oman.antibiotic resistant bacteria were studied as bio-indicators of marine polluted effluents during egg-laying in green turtles. a non-invasive procedure for sampling oviductal fluid was used to test for exposure of turtles to pollution in ras al-hadd, oman, which is one of the most important nesting beaches in the world. each sample was obtained by inserting a 15 cm sterile swab gently into the cloacal vent as the sphincter muscle is relaxed and the cloacal lining is unfolded to the outside. forty ...201021237506
comprehensive approaches to molecular biomarker discovery for detection and identification of cronobacter spp. (enterobacter sakazakii) and salmonella spp.cronobacter spp. (formerly enterobacter sakazakii) and salmonella spp. are increasingly implicated internationally as important microbiological contaminants in low-moisture food products, including powdered infant formula. estimates indicate that 40 to 80% of infants infected with cronobacter sakazakii and/or salmonella in the united states may not survive the illness. a systematic approach, combining literature-based data mining, comparative genome analysis, and the direct sequencing of pcr pro ...201121239552
the pathogenicity of an enteric citrobacter rodentium infection is enhanced by deficiencies in the antioxidants selenium and vitamin e.the pathogenesis of a citrobacter rodentium infection was evaluated in mice fed diets with a single deficiency in either selenium or vitamin e or with a double deficiency in both selenium and vitamin e compared to mice on nutritionally adequate diets. mice fed the selenium- and vitamin e-deficient diet for 6 weeks had increased loads of c. rodentium in the colon and spleen, which were not observed in mice fed either of the singly deficient diets or the adequate diet. infected mice fed the doubly ...201121245271
in vitro and in vivo properties of bal30376, a β-lactam and dual beta-lactamase inhibitor combination with enhanced activity against gram-negative bacilli that express multiple β-lactamases.bal30376 is a triple combination comprising a siderophore monobactam, bal19764; a novel bridged monobactam, bal29880, which specifically inhibits class c β-lactamases; and clavulanic acid, which inhibits many class a and some class d β-lactamases. the mic(90) was ≤ 4 μg/ml (expressed as the concentration of bal19764) for most species of the enterobacteriaceae family, including strains that produced metallo-β-lactamases and were resistant to all of the other β-lactams tested. the mic(90) for sten ...201121245441
structure of pdut, a trimeric bacterial microcompartment protein with a 4fe-4s cluster-binding site.propanediol metabolism in citrobacter freundii occurs within a metabolosome, a subcellular proteinaceous bacterial microcompartment. the propanediol-utilization (pdu) microcompartment shell is constructed from thousands of hexagonal-shaped protein complexes made from seven different types of protein subunit. here, the structure of the bacterial microcompartment protein pdut, which has a tandem structural repeat within the subunit and forms trimers with pseudo-hexagonal symmetry, is reported. thi ...201121245529
the unexpected link between infection-induced apoptosis and a th17 immune response.microbial pathogens can initiate momp in host cells and as such, initiate the mitochondrial pathway of apoptosis. innate immune recognition of cells dying in this way by infection-induced apoptosis would involve recognition of ligands derived from the apoptotic host cell simultaneously with those derived from the infecting pathogen. the resultant signal transduction pathways engaged direct dcs to concomitantly synthesize tgf-ß and il-6, two cytokines that subsequently favor the differentiation o ...201121248151
evaluation of the immunogenicity and biological activity of the citrobacter freundii vi-crm197 conjugate as a vaccine for salmonella enterica serovar typhi.typhoid fever remains a major health problem in developing countries. young children are at high risk, and a vaccine effective for this age group is urgently needed. purified capsular polysaccharide from salmonella enterica serovar typhi (vi) is licensed as a vaccine, providing 50 to 70% protection in individuals older than 5 years. however, this vaccine is ineffective in infants. vi conjugated to a carrier protein (i.e., an exoprotein a mutant from pseudomonas aeruginosa [repa]) is highly immun ...201121248155
virulence regulation in citrobacter rodentium: the art of timing.the mouse enteric pathogen citrobacter rodentium, like its human counterpart, enteropathogenic escherichia coli, causes attaching and effacing lesions in the intestinal epithelium of its host. this phenotype requires virulence factors encoded by the locus for enterocyte effacement (lee) pathogenicity island. for timely expression of these virulence determinants at the site of infection and for efficient delivery of some virulence factors into epithelial cells, c. rodentium utilizes a positive re ...201021255326
detection of aaca-aphd, qaced1, mara, flor, and teta genes from multidrug-resistant bacteria: comparative analysis of real-time multiplex pcr assays using evagreen(®) and sybr(®) green i dyes.we have developed multiplex real-time pcr assays that utilize dna-intercalating dyes, sybr green i (sg) and evagreen (eg), with two primer sets (set 1=qaced1, teta and aaca-aphd; set 2=teta, mara, and flor) to simultaneously amplify the qaced1, teta, aaca-aphd, mara, and flor genes. validity of the multiplex pcr assays was confirmed by testing 83 bacterial isolates, including staphylococcus aureus (28 isolates), enterococcus spp. (17 isolates), salmonella enterica serovar typhimurium (8 isolates ...201121256956
characterization of swarming motility in citrobacter freundii.bacterial swarming motility is a flagella-dependent translocation on the surface environment. it has received extensive attention as a population behavior involving numerous genes. here, we report that citrobacter freundii, an opportunistic pathogen, exhibits swarming movement on a solid medium surface with appropriate agar concentration. the swarming behavior of c. freundii was described in detail. insertional mutagenesis with transposon mini-tn5 was carried out to discover genetic determinants ...201121261700
is selective digestive decontamination useful in controlling aerobic gram-negative bacilli producing extended spectrum beta-lactamases?to identify outbreak episodes of either carriage or infection due to extended spectrum beta-lactamases producing aerobic gram-negative bacilli (agnb-esbl); to establish whether agnb-esbl, sensitive to tobramycin, become resistant over time; and to evaluate the impact of selective decontamination of the digestive tract (sdd) on abnormal carriage of agnb-esbl.201121268690
autogenous transcriptional regulation of the rega gene, encoding an arac-like, essential virulence regulator in citrobacter rodentium.we identified several promoters responsible for the expression of rega, which encodes a global virulence regulator in citrobacter rodentium. expression of some of the promoters was strongly autoactivated by rega in conjunction with bicarbonate. biochemical and mutational analyses were used to determine the consensus sequence of the rega-binding sites.201121278290
incidence of fever and bacteriemia following flexible fiberoptic bronchoscopy: a prospective study.the latest american heart association (aha) statement for preventing infectious endocarditis, has not recommended prophylactic antibiotic therapy prior to fiberoptic bronchoscopy (fb) except for patients with preexisting predisposing cardiac conditions. our aim was to determine the incidence of bacteriemia and fever following fb in our experience and compare with those which have been mentioned in aha guideline as well as other studies. venous blood of 85 consecutive patients was evaluated for b ...201021287478
antimicrobial susceptibility and multiplex pcr screening of ampc genes from isolates of enterobacter cloacae, citrobacter freundii, and serratia marcescens.the emergence of multiple drug resistance in enterobacteriaceae is of particular concern. the aim of this study was to evaluate the antimicrobial susceptibility and screen for the ampc gene in three members of the enterobacteriaceae family (enterobacter cloacae, citrobacter freundii, and serratia marcescens) found at taichung veterans general hospital during the past 5 years using multiplex polymerase chain reaction (pcr).201021291844
microbial evaluation and public health implications of urine as alternative therapy in clinical pediatric cases: health implication of urine therapy.cultural means of pediatric treatment during ill health is a mainstay in africa, and though urine has been known to contain enteric pathogens, urine therapy is still culturally applicable in some health conditions and also advocated as alternative therapy. the study therefore, is to evaluate the microbial contents and safety of urine.201021293739
diversity of aromatic ring-hydroxylating dioxygenase gene in citrobacter.genetic and functional diversity of citrobacter spp. for their abilities to degrade aromatic compounds was evaluated to develop mixed cultures or a consortium for bioremediation technology. thirty citrobacter strains isolated from various effluent treatment plants were found to degrade a range of aromatic compounds: phenol, benzoate, hydroxy benzoic acid and biotransform mono-chlorophenols and di-chlorophenol within 24 to 48 h of incubation at 30 °c. sequence similarity and phylogeny of the arhd ...201121295975
citrobacter freundii infection after acute necrotizing pancreatitis in a patient with a pancreatic pseudocyst: a case report. 201121299889
bacterial species associated with sound and botrytis-infected grapes from a greek vineyard.grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. in the present study, bacterial species associated with sound and botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. isolates were identified by pcr-denaturing gradient gel electrophoresis (pcr-dgge) and sequence analysis of partial 16s rrna gene. comparable counts were recorded between botrytis-infected and sound grape samples. in ...201121315469
antibiotic treatment alters the colonic mucus layer and predisposes the host to exacerbated citrobacter rodentium-induced colitis.antibiotics are often used in the clinic to treat bacterial infections, but the effects of these drugs on microbiota composition and on intestinal immunity are poorly understood. citrobacter rodentium was used as a model enteric pathogen to investigate the effect of microbial perturbation on intestinal barriers and susceptibility to colitis. streptomycin and metronidazole were used to induce alterations in the composition of the microbiota prior to infection with c. rodentium. metronidazole pret ...201121321077
identification and characterization of cri1, a locus controlling mortality during citrobacter rodentium infection in mice.infection of inbred mouse strains with citrobacter rodentium represents an ideal model to reveal the genetic factors controlling host resistance to noninvasive enteric bacterial pathogens. we have chosen a positional cloning approach to identify putative gene(s) that control the known difference in survival between resistant c57bl/6j and susceptible c3h/hej and c3h/heouj mice. our work has identified one major locus within proximal chromosome 15 that is responsible for the marked susceptibility ...201121326319
isolation of a selected microbial consortium capable of degrading methyl parathion and p-nitrophenol from a contaminated soil site.a bacterial consortium with the ability to degrade methyl parathion and p-nitrophenol, using these compounds as the only carbon source, was obtained by selective enrichment in a medium with methyl parathion. samples were taken from moravia, medellin; an area that is highly contaminated, owing to the fact that it was used as a garbage dump from 1974 to 1982. acinetobacter sp, pseudomonas putida, bacillus sp, pseudomonas aeruginosa citrobacter freundii, stenotrophomonas sp, flavobacterium sp, prot ...201121328125
molecular characterization of antimicrobial resistance in gram-negative bacteria isolated from bovine mastitis in egypt.the aim of this study was to characterize the genetic basis of multidrug resistance in gram-negative bacteria isolated from bovine mastitis cases in egypt. multidrug resistance phenotypes were found in 34 of 112 (30.4%) gram-negative bacterial isolates, which harbored at least one antimicrobial resistance gene. the most prevalent multidrug-resistant (mdr) species were enterobacter cloacae (8 isolates, 7.1%), klebsiella pneumoniae (7 isolates, 6.3%), klebsiella oxytoca (7 isolates, 6.3%), escheri ...201121338385
akara ogbomoso: microbiological examination and identification of hazards and critical control points.akara ogbomoso was examined toward the establishment of hazard analysis and critical control point (haccp). the akara was produced in residential buildings with the attendant consequence of contamination. there was ample growth of aerobes, coliforms, staphylococci, shigella and yeast/mold from the samples, water and cowpea pastes. microbial contaminations occur through the processing, which can be corrected through education by adopting good hygienic and manufacturing practices. the critical con ...201021339157
lymphotoxin-ß receptor-independent development of intestinal il-22-producing nkp46+ innate lymphoid cells.the natural cytotoxicity receptor nkp46 is an activating receptor expressed by several distinct innate lymphoid cell (ilc) subsets, including nk cells, some ?d t cells and intestinal ror?t(+) il-22(+) cells (ncr22 cells, il-22-producing nkp46(+) cell). ncr22 cells may play a role in mucosal barrier function through il-22-mediated production of anti-bacterial peptides from intestinal epithelial cells. previous studies identified a predominant proportion of ncr22 cells in gut cryptopatches (cp), l ...201121341264
epithelial phosphatidylinositol-3-kinase signaling is required for ß-catenin activation and host defense against citrobacter rodentium infection.citrobacter rodentium infection of mice induces cell-mediated immune responses associated with crypt hyperplasia and epithelial ß-catenin signaling. recent data suggest that phosphatidylinositol-3-kinase (pi3k)/akt signaling cooperates with wnt to activate ß-catenin in intestinal stem and progenitor cells through phosphorylation at ser552 (p-ß-catenin(552)). our aim was to determine whether epithelial pi3k/akt activation is required for ß-catenin signaling and host defense against c. rodentium. ...201121343355
diarrheagenic escherichia coli in acute gastroenteritis in infants in north-west italy.enteropathogenic escherichia coli may cause diarrhoea in infancy, but it is not routinely detected and regarded as a major causative agent. the aim of the present study was to estimate the incidence of enteropathogenic e. coli infection and to investigate its epidemiology and pathogenesis from faecal specimens in infants hospitalized for acute gastroenteritis. between march 2008 and june 2009, faecal samples were collected and examined to recognize diarrhoeal aetiology, especially for e. coli, b ...201121344146
prevalence of pseudomonas aeruginosa in post-operative wound infection in a referral hospital in haryana, india.the objective of our study was to determine the prevalence of pseudomonas aeruginosa in the isolates of postoperative wound and its susceptibility pattern to commonly used antibiotics.201021346900
in vitro activity of ceftobiprole against frequently encountered aerobic and facultative gram-positive and gram-negative bacterial pathogens: results of the canward 2007-2009 study.the in vitro activity of ceftobiprole was evaluated against 15 011 clinical isolates obtained from patients in canadian hospitals between 2007 and 2009. all staphylococcus aureus were susceptible to ceftobiprole (mic(90)'s for methicillin-susceptible staphylococcus aureus and methicillin-resistant staphylococcus aureus of = 1 µg/ml and 2 µg/ml, respectively). ceftobiprole was active against penicillin-susceptible streptococcus pneumoniae (mic(90), = 0.06 µg/ml), penicillin-resistant streptococcu ...201121353964
fecal carriage of extended-spectrum ß-lactamase-producing enterobacteriaceae in swine and cattle at slaughter in switzerland.during the past decade, extended-spectrum ß-lactamase (esbl)-producing enterobacteriaceae have become a matter of great concern in human medicine. esbl-producing strains are found in the community, not just in hospital-associated patients, which raises a question about possible reservoirs. recent studies describe the occurrence of esbl-producing enterobacteriaceae in meat, fish, and raw milk; therefore, the impact of food animals as reservoirs for and disseminators of such strains into the food ...201121375882
absence of pmrab-mediated phosphoethanolamine modifications of citrobacter rodentium lipopolysaccharide affects outer membrane integrity.the pmrab two-component system of enterobacteria regulates a number of genes whose protein products modify lipopolysaccharide (lps). the lps is modified during transport to the bacterial outer membrane (om). a subset of pmrab-mediated lps modifications consists of the addition of phosphoethanolamine (petn) to lipid a by pmrc and to the core by cpta. in salmonella enterica, petn modifications have been associated with resistance to polymyxin b and to excess iron. to investigate putative functions ...201121378194
difluoromethylornithine is a novel inhibitor of helicobacter pylori growth, caga translocation, and interleukin-8 induction.helicobacter pylori infects half the world's population, and carriage is lifelong without antibiotic therapy. current regimens prescribed to prevent infection-associated diseases such as gastroduodenal ulcers and gastric cancer can be thwarted by antibiotic resistance. we reported that administration of 1% d,l-+¦-difluoromethylornithine (dfmo) to mice infected with h. pylori reduces gastritis and colonization, which we attributed to enhanced host immune response due to inhibition of macrophage o ...201121386987
comparison of the diversity of the bacterial communities in the intestinal tract of adult bactrocera dorsalis from three different populations.to (i) identify the bacterial communities in the gut of oriental fruit fly (bactrocera dorsalis) adult and (ii) determine whether the different surroundings and diets influence the bacteria composition.201121395953
evaluation of antimicrobial susceptibility for +¦-lactams against clinical isolates from 51 medical centers in japan (2008).this antimicrobial resistance surveillance study was performed in 51 medical centers in japan over an 11-year period. the susceptibilities of 4228 strains including escherichia coli (491 strains), klebsiella spp. (462 strains), enterobacter spp. (459 strains), citrobacter freundii (358 strains), indole-positive proteus spp. (386 strains), serratia spp. (443 strains), acinetobacter spp. (327 strains), pseudomonas aeruginosa (473 strains), oxacillin-susceptible staphylococcus aureus (481 strains), ...201121396543
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