| [calcified foci in school pupils as an index of prevalence of tuberculosis]. | | 2015 | 4643908 |
| the immunological response of cba mice to trypanosoma musculi. i. initial control of the infection and the effect of t-cell deprivation. | | 2015 | 4619878 |
| [postoperative development of function in patients with bronchial carcinoma following lung resection: long term prognosis]. | | 2015 | 4641501 |
| perspectives in neuro-ophthalmology. | | 2015 | 4606901 |
| [caucasian period of the activity of v. s. bobrovskiĭ (on the 100th anniversary of his birth)]. | | 2015 | 4592872 |
| [proceedings: earlier diagnosis of endometrium carcinoma]. | | 2015 | 4801456 |
| [proceedings: earlier diagnosis of endometrium carcinoma]. | | 2015 | 4801456 |
| [studies on the determination and evaluation of metamorphopsias. iv. metamorphopsias in correlation to coloured patterns of testcards]. | | 2015 | 4537211 |
| [surgical treatment of chronic nonspecific lung diseases]. | | 2015 | 4662451 |
| [methods of osteosynthesis in the jaw region]. | | 2015 | 4513145 |
| temporal lobe epilepsy due to drug withdrawal. | | 2015 | 4509764 |
| the modulation transfer function as a performance specification for ophthalmic lens and protective devices. | | 2015 | 4502357 |
| complex odontoma in the maxillary sinus. | | 2015 | 4518028 |
| [bleeding in oral surgery]. | | 2015 | 4542263 |
| [role of the sanitary-epidemiological station in the elaboration and introduction of physiologically based recommendations in industrial enterprises]. | | 2015 | 4464220 |
| [comparison of therapeutic results in inflammations of the adnexa obtained with trypsin, adnexbacterin, floristen and betalactin (author's transl)]. | | 2015 | 4800989 |
| loudness discomfort level: selected methods and stimuli. | | 2015 | 4414517 |
| [problems of prosthesis fitting following amputations of the lower limbs. observations from the lublin province]. | | 2015 | 4755334 |
| the mechanism of substrate inhibition of the esterase activity of carboxypeptidase a. | | 2015 | 4342336 |
| [computer scintigraphy of pulmonary circulation. 3. perfusion pattern in obstructive syndrome and relations to lung function and hemodynamics of the pulmonary circulation]. | | 2015 | 4339695 |
| role of inhibition in the development of motor co-ordination in early childhood. | | 2015 | 4338268 |
| endolymphatic radiotherapy (ert) for malignant lymphomas: indications and long-term results. | | 2015 | 4313626 |
| is jump to population-explosion bandwagon justified? | | 2016 | 5009981 |
| clinical trial of clomacran phosphate (skf-14336) in chronic psychotic male patients. | | 2016 | 4987347 |
| impact of efforts to increase participation in repetitive screenings for early breast cancer detection. | | 2016 | 5011163 |
| [aspects of long term care. 1]. | | 2016 | 4971091 |
| [early postoperative rehabilitation after maxillary resection]. | | 2016 | 4951118 |
| [glucagon and gastric secretion]. | | 2016 | 4914558 |
| [the medicine of carlos de sigüenza and sister juana inés de la cruz. contribution to the baroque thought of 17th century mexico]. | | 2016 | 4913425 |
| james hardy and the devonshire colic. | | 2016 | 4890043 |
| [surgery of cancer of the lower esophagus]. | | 2016 | 4881601 |
| occurrence of gas phase ammonolysis during chemical ionization mass spectrometry. | | 2016 | 4847773 |
| [measurement of central venous pressure: only in recumbent position?]. | | 2016 | 4829023 |
| [subphrenic abscess and its x-ray differential diagnosis]. | | 2016 | 5076712 |
| [circumstantial study of accidents in minors under 15 years of age (study of 100 cases)]. | | 2016 | 4791562 |
| [the bioelectrical activity of the muscles in patients following poliomyelitis]. | | 2016 | 4775888 |
| [determination of the weight and hydration of bacteriophage sd from x-ray small-angle scattering data]. | | 2016 | 4748892 |
| primary angle-closure glaucoma in urbanized south african caucasoid and negroid communities. | | 2016 | 4725852 |
| vascuar and neurological changes in early diabetes. | | 2016 | 4720362 |
| contribution of six flagellin genes to the flagellum biogenesis of vibrio vulnificus and in vivo invasion. | vibrio vulnificus is a halophilic pathogenic bacterium that is motile due to the presence of a single polar flagellum. v. vulnificus possesses a total of six flagellin genes organized into two loci (flafba and flacde). we proved that all six of the flagellin genes were transcribed, whereas only five (flaa, -b, -c, -d, and -f) of the six flagellin proteins were detected. to understand roles of the six v. vulnificus flagellins in motility and virulence, mutants with single and multiple flagellin d ... | 2014 | 24101693 |
| starvation induces phenotypic diversification and convergent evolution in vibrio vulnificus. | starvation is a common stress experienced by bacteria living in natural environments and the ability to adapt to and survive intense stress is of paramount importance for any bacterial population. a series of starvation experiments were conducted using v. vulnificus 93u204 in phosphate-buffered saline and seawater. the starved population entered the death phase during the first week and approximately 1% of cells survived. after that the population entered a long-term stationary phase, and could ... | 2014 | 24551129 |
| host-like carbohydrates promote bloodstream survival of vibrio vulnificus in vivo. | sialic acids are found on all vertebrate cell surfaces and are part of a larger class of molecules known as nonulosonic acids. many bacterial pathogens synthesize related nine-carbon backbone sugars; however, the role(s) of these non-sialic acid molecules in host-pathogen interactions is poorly understood. vibrio vulnificus is the leading cause of seafood-related death in the united states due to its ability to quickly access the host bloodstream, which it can accomplish through gastrointestinal ... | 2015 | 26015477 |
| insight into the evolution of vibrio vulnificus biotype 3's genome. | vibrio vulnificus is an aquatic bacterium and an important human pathogen. strains of v. vulnificus are biochemically classified into three biotypes. the newly emerged biotype 3 appears to be rather clonal and geographically restricted to israel, where it caused an outbreak of wound infections and bacteremia. to understand the evolution of the bacterium's genome, we sequenced and analyzed the genome of biotype 3 strain vvyb1(bt3), and then conducted a microbial environmental survey of the hypoth ... | 2013 | 24385974 |
| activities of alkyl hydroperoxide reductase subunits c1 and c2 of vibrio parahaemolyticus against different peroxides. | alkyl hydroperoxide reductase subunit c gene (ahpc) functions were characterized in vibrio parahaemolyticus, a commonly occurring marine food-borne enteropathogenic bacterium. two ahpc genes, ahpc1 (vpa1683) and ahpc2 (vp0580), encoded putative two-cysteine peroxiredoxins, which are highly similar to the homologous proteins of vibrio vulnificus. the responses of deletion mutants of ahpc genes to various peroxides were compared with and without gene complementation and at different incubation tem ... | 2014 | 25239899 |
| vibrio vulnificus secretes an insulin-degrading enzyme that promotes bacterial proliferation in vivo. | we describe a novel insulin-degrading enzyme, sidc, that contributes to the proliferation of the human bacterial pathogen vibrio vulnificus in a mouse model. sidc is phylogenetically distinct from other known insulin-degrading enzymes and is expressed and secreted specifically during host infection. purified sidc causes a significant decrease in serum insulin levels and an increase in blood glucose levels in mice. a comparison of mice infected with wild type v. vulnificus or an isogenic sidc-del ... | 2015 | 26041774 |
| cloning, crystallization and preliminary x-ray diffraction analysis of an intact dna methyltransferase of a type i restriction-modification enzyme from vibrio vulnificus. | independently of the restriction (hsdr) subunit, the specificity (hsds) and methylation (hsdm) subunits interact with each other, and function as a methyltransferase in type i restriction-modification systems. a single gene that combines the hsds and hsdm subunits in vibrio vulnificus yj016 was expressed and purified. a crystal suitable for x-ray diffraction was obtained from 25%(w/v) polyethylene glycol monomethylether 5000, 0.1 m hepes ph 8.0, 0.2 m ammonium sulfate at 291 k by hanging-drop va ... | 2014 | 24699746 |
| crystal structure of the full-length atpase gspe from the vibrio vulnificus type ii secretion system in complex with the cytoplasmic domain of gspl. | the type ii secretion system (t2ss) is present in many gram-negative bacteria and is responsible for secreting a large number of folded proteins, including major virulence factors, across the outer membrane. the t2ss consists of 11-15 different proteins most of which are present in multiple copies in the assembled secretion machinery. the atpase gspe, essential for the functioning of the t2ss, contains three domains (n1e, n2e and cte) of which the n1e domain is associated with the cytoplasmic do ... | 2014 | 25092625 |
| iron and quorum sensing coordinately regulate the expression of vulnibactin biosynthesis in vibrio vulnificus. | vibrio vulnificus is a halophilic marine pathogen associated with human diseases such as septicemia and serious wound infections. genes vvsa and vvsb, which are co-transcribed and encode a member of the nonribosomal peptide synthase family, are required for vulnibactin biosynthesis in v. vulnificus. in this study, we found that quorum sensing represses the transcription of a vvsab-lux reporter fusion. gel shift assay and dnasei footprinting experiments show that the main regulator of quorum sens ... | 2012 | 22696215 |
| molecular characterization of vulnibactin biosynthesis in vibrio vulnificus indicates the existence of an alternative siderophore. | vibrio vulnificus is a halophilic estuarine bacterium that causes fatal septicemia and necrotizing wound infections in humans. virulent v. vulnificus isolates produce a catechol siderophore called vulnibactin, made up of one residue of 2, 3-dihydroxybenzoic acid (2, 3-dhba) and two residues of salicylic acid (sa). vulnibactin biosynthetic genes (vv2_0828 to vv2_0844) are clustered at one locus of chromosome 2, expression of which is significantly up-regulated in vivo. in the present study, we de ... | 2014 | 24478763 |
| the cababc operon essential for biofilm and rugose colony development in vibrio vulnificus. | a transcriptome analysis identified vibrio vulnificus cababc genes which were preferentially expressed in biofilms. the cababc genes were transcribed as a single operon. the caba gene was induced by elevated 3',5'-cyclic diguanylic acid (c-di-gmp) and encoded a calcium-binding protein caba. comparison of the biofilms produced by the caba mutant and its parent strain jn111 in microtiter plates using crystal-violet staining demonstrated that caba contributed to biofilm formation in a calcium-depen ... | 2015 | 26406498 |
| the makes caterpillars floppy (mcf)-like domain of vibrio vulnificus induces mitochondrion-mediated apoptosis. | the multifunctional-autoprocessing repeats-in-toxin (martxvv) toxin of vibrio vulnificus plays a significant role in the pathogenesis of this bacterium through delivery of up to five effector domains to the host cells. previous studies have established that the martxvv toxin is linked to v. vulnificus dependent induction of apoptosis, but the region of the large multifunction protein essential for this activity was not previously identified. recently, we showed that the makes caterpillar floppy- ... | 2015 | 26351282 |
| predatory bacteriovorax communities ordered by various prey species. | the role of predation in altering microbial communities has been studied for decades but few examples are known for bacterial predators. bacteriovorax are halophilic prokaryotes that prey on susceptible gram-negative bacteria. we recently reported novel observations on the differential selection of bacteriovorax phylotypes by two different prey, vibrio parahaemolyticus and vibrio vulnificus. however, the conclusion is restricted by the limited number of prey tested. in this study, we have conduc ... | 2012 | 22461907 |
| development of a quadruplex loop-mediated isothermal amplification assay for field detection of four vibrio species associated with fish disease. | a quadruplex loop-mediated isothermal amplification (lamp) method was developed to detect four vibrio species, including vibrio ichthyoenteri, vibrio parahaemolyticus, vibrio scophthalmi, and vibrio vulnificus, simultaneously. four sets of species-specific primers were designed with different restriction sites contained in the inner primers. the quadruplex lamp method could distinguish four vibrio species via the subsequent restriction enzyme analysis. the sensitivity of the quadruplex lamp meth ... | 2016 | 27468405 |
| genotypic diversity and population structure of vibrio vulnificus strains isolated in taiwan and korea as determined by multilocus sequence typing. | the genetic diversity and population structure of vibrio vulnificus isolates from korea and taiwan were investigated using pcr-based assays targeting putative virulence-related genes and multilocus sequence typing (mlst). box-pcr genomic fingerprinting identified 52 unique genotypes in 84 environmental and clinical v. vulnificus isolates. the majority (> 50%) of strains had pathogenic genotypes for all loci tested; moreover, many environmental strains had pathogenic genotypes. although significa ... | 2015 | 26599487 |
| induced autoprocessing of the cytopathic makes caterpillars floppy-like effector domain of the vibrio vulnificus martx toxin. | the multifunctional-autoprocessing repeats-in-toxin (martx(vv)) toxin that harbours a varied repertoire of effector domains is the primary virulence factor of vibrio vulnificus. although ubiquitously present among biotype i toxin variants, the 'makes caterpillars floppy-like' effector domain (mcf(vv)) is previously unstudied. using transient expression and protein delivery, mcf(vv) and mcf(ah) from the aeromonas hydrophila martx(ah)) toxin are shown for the first time to induce cell rounding. al ... | 2015 | 25912102 |
| role of acsr in expression of the acetyl-coa synthetase gene in vibrio vulnificus. | vars/vara is one of the global factors regulating diverse aspects of the metabolism and virulence of bacteria including pathogenic vibrio spp. an experiment to identify the vars/vara-regulon in v. vulnificus revealed that a putative luxr-type transcriptional regulator was down-regulated in δvara mutant. to investigate the roles of this regulatory cascade, the target gene regulated by a luxr-regulator was identified and its expression was characterized. | 2015 | 25887971 |
| identification of the target dna sequence and characterization of dna binding features of hlyu, and suggestion of a redox switch for hlya expression in the human pathogen vibrio cholerae from in silico studies. | hlyu, a transcriptional regulator common in many vibrio species, activates the hemolysin gene hlya in vibrio cholerae, the rtxa1 operon in vibrio vulnificus and the genes of plp-vah1 and rtxachbde gene clusters in vibrio anguillarum. the protein is also proposed to be a potential global virulence regulator for v. cholerae and v. vulnificus. mechanisms of gene control by hlyu in v. vulnificus and v. anguillarum are reported. however, detailed elucidation of the interaction of hlyu in v. cholerae ... | 2015 | 25605793 |
| purification and host specificity of predatory halobacteriovorax isolates from seawater. | halobacteriovorax (formerly bacteriovorax) is a small predatory bacterium found in the marine environment and modulates bacterial pathogens in shellfish. four strains of halobacteriovorax originally isolated in vibrio parahaemolyticus o3:k6 host cells were separated from their prey by an enrichment-filtration-dilution technique for specificity testing in other bacteria. this technique was essential, since 0.45-μm filtration alone was unable to remove infectious vibrio minicells, as determined by ... | 2016 | 26590288 |
| the tonb3 system in the human pathogen vibrio vulnificus is under the control of the global regulators lrp and cyclic amp receptor protein. | tonb systems transduce the proton motive force of the cytoplasmic membrane to energize substrate transport through a specific tonb-dependent transporter across the outer membrane. vibrio vulnificus, an opportunistic marine pathogen that can cause a fatal septicemic disease in humans and eels, possesses three tonb systems. while the tonb1 and tonb2 systems are iron regulated, the tonb3 system is induced when the bacterium grows in human serum. in this work we have determined the essential roles o ... | 2012 | 22307757 |
| structural insights into the regulation of sialic acid catabolism by the vibrio vulnificus transcriptional repressor nanr. | pathogenic and commensal bacteria that experience limited nutrient availability in their host have evolved sophisticated systems to catabolize the mucin sugar n-acetylneuraminic acid, thereby facilitating their survival and colonization. the correct function of the associated catabolic machinery is particularly crucial for the pathogenesis of enteropathogenic bacteria during infection, although the molecular mechanisms involved with the regulation of the catabolic machinery are unknown. this stu ... | 2013 | 23832782 |
| host-nonspecific iron acquisition systems and virulence in the zoonotic serovar of vibrio vulnificus. | the zoonotic serovar of vibrio vulnificus (known as biotype 2 serovar e) is the etiological agent of human and fish vibriosis. the aim of the present work was to discover the role of the vulnibactin- and hemin-dependent iron acquisition systems in the pathogenicity of this zoonotic serovar under the hypothesis that both are host-nonspecific virulence factors. to this end, we selected three genes for three outer membrane receptors (vuua, a receptor for ferric vulnibactin, and hupa and hutr, two h ... | 2014 | 24478087 |
| monoclonal antibodies against vibrio vulnificus rtxa1 elicit protective immunity through distinct mechanisms. | vibrio vulnificus causes rapidly progressing septicemia with an extremely high mortality rate (≥50%), even with aggressive antibiotic treatment. the bacteria secrete multifunctional autoprocessing repeats-in-toxin (martx) toxins, which are involved in the pathogenesis of gram-negative vibrio species. recently, we reported that immunization with the c-terminal region of v. vulnificus rtxa1/martxvv, rtxa1-c, elicits a protective immune response against v. vulnificus through a poorly defined mechan ... | 2014 | 25156730 |
| vibrio vulnificus biotype 3 multifunctional autoprocessing rtx toxin is an adenylate cyclase toxin essential for virulence in mice. | vibrio vulnificus is an environmental organism that causes both food-borne and wound infections with high morbidity and mortality in humans. the annual incidence and global distribution of infections associated with this pathogen are increasing with climate change. in the late 1990s, an outbreak of tilapia-associated wound infections in israel was linked to a previously unrecognized variant of v. vulnificus designated biotype 3. the sudden emergence and clonality of the outbreak suggest that thi ... | 2014 | 24614656 |
| distinct roles of the repeat-containing regions and effector domains of the vibrio vulnificus multifunctional-autoprocessing repeats-in-toxin (martx) toxin. | vibrio vulnificus is a seafood-borne pathogen that destroys the intestinal epithelium, leading to rapid bacterial dissemination and death. the most important virulence factor is the multifunctional-autoprocessing repeats-in-toxin (martx) toxin comprised of effector domains in the center region flanked by long repeat-containing regions which are well conserved among martx toxins and predicted to translocate effector domains. here, we examined the role of the repeat-containing regions using a modi ... | 2015 | 25827415 |
| iscr is a global regulator essential for pathogenesis of vibrio vulnificus and induced by host cells. | a mutant that exhibited less cytotoxic activity toward int-407 human intestinal epithelial cells than the wild type was screened from a random transposon mutant library of vibrio vulnificus, and an open reading frame encoding an fe-s cluster regulator, iscr, was identified using a transposon-tagging method. a mutational analysis demonstrated that iscr contributes to mouse mortality as well as cytotoxicity toward the int-407 cells, indicating that iscr is essential for the pathogenesis of v. vuln ... | 2014 | 24478072 |
| norovirus (nov) specific protective immune responses induced by recombinant p dimer vaccine are enhanced by the mucosal adjuvant flab. | noroviruses (novs) are a major cause of childhood gastroenteritis and foodborne diseases worldwide. lack of appropriate animal models or cell-based culture systems makes the development and evaluation of nov-specific vaccines a daunting task. vp1 is the major capsid protein of the novs that acts as a binding motif to human histo-blood group antigens (hbgas) through its protruding 2 (p2) domain and can serve as a protective antigen candidate for vaccine development. | 2016 | 27184355 |
| evidence of antibacterial activities in peptide fractions originating from snow crab (chionoecetes opilio) by-products. | antibacterial peptide fractions generated via proteolytic processing of snow crab by-products exhibited activity against gram-negative and gram-positive bacteria. among the bacterial strains tested, peptide fractions demonstrated inhibitory activity against the gram-negative bacteria such as aeromonas caviae, aeromonas hydrophila, campylobacter jejuni, listonella anguillarum, morganella morganii, shewanella putrefasciens, vibrio parahaemolyticus and vibrio vulnificus and against a few gram-posit ... | 2010 | 26781242 |
| pathogenic mechanisms of actin cross-linking toxins: peeling away the layers. | actin cross-linking toxins are produced by gram-negative bacteria from vibrio and aeromonas genera. the toxins were named actin cross-linking domains (acd), since the first and most of the subsequently discovered acds were found as effector domains in larger martx and vgrg toxins. among recognized human pathogens, acd is produced by vibrio cholerae, vibrio vulnificus, and aeromonas hydrophila. upon delivery to the cytoplasm of a host cell, acd covalently cross-links actin monomers into non-polym ... | 2017 | 27858184 |
| o-heterocyclic derivatives with antibacterial properties from marine bacterium bacillus subtilis associated with seaweed, sargassum myriocystum. | the brown seaweed, sargassum myriocystum associated with heterotrophic bacterium, bacillus subtilis mtcc 10407 (jf834075) exhibited broad-spectra of potent antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio vulnificus, and vibrio parahaemolyticus. b. subtilis mtcc 10407 was found to be positive for polyketide synthetase (pks) gene, and therefore, was considered to characterize secondary metabolites bearing polyketide backbone. using bioassay-guided fractionation, t ... | 2017 | 27624095 |
| actin-directed toxin. acd toxin-produced actin oligomers poison formin-controlled actin polymerization. | the actin cross-linking domain (acd) is an actin-specific toxin produced by several pathogens, including life-threatening spp. of vibrio cholerae, vibrio vulnificus, and aeromonas hydrophila. actin cross-linking by acd is thought to lead to slow cytoskeleton failure owing to a gradual sequestration of actin in the form of nonfunctional oligomers. here, we found that acd converted cytoplasmic actin into highly toxic oligomers that potently "poisoned" the ability of major actin assembly proteins, ... | 2015 | 26228148 |
| a novel recombinant bivalent outer membrane protein of vibrio vulnificus and aeromonas hydrophila as a vaccine antigen of american eel (anguilla rostrata). | the immogenicity of a novel vaccine antigen was evaluated after immunized american eels (anguilla rostrata) with a recombinant bivalent expressed outer membrane protein (omp) of vibrio vulnificus and aeromonas hydrophila. three groups of eels were intraperitoneal (i.p) injected with phosphate-buffered saline (pbs group), formaline-killed-whole-cell (fkc) of a. hydrophila and v. vulnificus (fkc group) or the bivalent omp (omp group). on 14, 21, 28 and 42 days post-vaccination respectively, prolif ... | 2015 | 25655329 |
| emerging causes of superficial and invasive infections following marine injuries and exposures. | soft tissue bacterial infections following aquatic animal bites, stings, and minor injuries occur commonly and usually on the extremities in fishermen and beachgoers worldwide after freshwater and saltwater exposures. louisiana has more tidal, saltwater, and brackish water shorelines (more than 7,000 miles) than any other state, including alaska and hawaii. as a result, louisiana residents are often exposed to marine pathogens when fishing or working offshore or when enjoying louisiana's miles o ... | 2017 | 25075726 |
| cytotoxicity of the vibrio vulnificus martx toxin effector duf5 is linked to the c2a subdomain. | the multifunctional-autoprocessing repeats-in-toxin (martx) toxins are bacterial protein toxins that serve as delivery platforms for cytotoxic effector domains. the domain of unknown function in position 5 (duf5) effector domain is present in at least six different species' martx toxins and as a hypothetical protein in photorhabdus spp. its presence increases the potency of the vibrio vulnificus martx toxin in mouse virulence studies, indicating duf5 directly contributes to pathogenesis. in this ... | 2014 | 24935440 |
| thermodynamic properties of the effector domains of martx toxins suggest their unfolding for translocation across the host membrane. | martx (multifunctional autoprocessing repeats-in-toxin) family toxins are produced by vibrio cholerae, vibrio vulnificus, aeromonas hydrophila and other gram-negative bacteria. effector domains of martx toxins cross the cytoplasmic membrane of a host cell through a putative pore formed by the toxin's glycine-rich repeats. the structure of the pore is unknown and the translocation mechanism of the effector domains is poorly understood. we examined the thermodynamic stability of the effector domai ... | 2014 | 24724536 |
| liposome-encapsulated cinnamaldehyde enhances zebrafish (danio rerio) immunity and survival when challenged with vibrio vulnificus and streptococcus agalactiae. | cinnamaldehyde, which is extracted from cinnamon, is a natural compound with activity against bacteria and a modulatory immune function. however, the antibacterial activity and immunostimulation of cinnamaldehyde in fish has not been well investigated due to the compound's poor water solubility. thus, liposome-encapsulated cinnamaldehyde (lec) was used to evaluate the effects of cinnamaldehyde on in vitro antibacterial activity against aquatic pathogens and in vivo immunity and protection parame ... | 2014 | 24632045 |
| skin and soft tissue infections following marine injuries and exposures in travelers. | bacterial skin and soft tissue infections (sstis) in travelers often follow insect bites and can present a broad spectrum of clinical manifestations ranging from impetigo to necrotizing cellulitis. significant sstis can also follow marine injuries and exposures in travelers, and the etiologies are often marine bacteria. | 2014 | 24628985 |
| bacterial infections from aquatic species: potential for and prevention of contact zoonoses. | as aquaculture production and the consumption of aquaculture products increase, the possibility of contracting zoonotic infections from either handling or ingesting these products also increases. the principal pathogens acquired topically from fish or shellfish through spine/pincer puncture or open wounds are aeromonas hydrophila, edwardsiella tarda, mycobacterium marinum, streptococcus iniae, vibrio vulnificus and v. damsela. these pathogens, which are all indigenous to the aquatic environment, ... | 2013 | 24547653 |
| structure of a periplasmic domain of the epsab fusion protein of the vibrio vulnificus type ii secretion system. | vibrio vulnificus utilizes the type ii secretion system (t2ss), culminating in a megadalton outer membrane complex called the secretin, to translocate extracellular proteins from the periplasmic space across the outer membrane. in aeromonas hydrophila, the general secretion pathway proteins exea and exeb form an inner membrane complex which interacts with peptidoglycan and is required for the assembly of the secretin composed of exed. in v. vulnificus, these two proteins are fused into one prote ... | 2013 | 23385451 |
| antibiofilm activity of dendrophthoe falcata against different bacterial pathogens. | dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. in this context, the validations of the traditional claim that the leaf extract of d. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. the bacterial biofilms were quantified by crystal violet staining. among the 17 bacterial pathogens screened ... | 2012 | 23115018 |
| development of a multiplex polymerase chain reaction to detect five common gram-negative bacteria of aquatic animals. | a multiplex polymerase chain reaction (m-pcr) technique was developed as a rapid and accurate diagnostic tool for identifying five major gram-negative bacilli -vibrio vulnificus, v. parahaemolyticus, aeromonas hydrophila, chryseobacterium meningosepticum and edwardsiella tarda- that cause major diseases in cultured aquatic animals in taiwan. the expected amplicons for v. vulnificus, v. parahaemolyticus, a. hydrophila, c. meningosepticum and e. tarda were 410, 368, 685, 180 and 230bp, respectivel ... | 2012 | 22571515 |
| antimicrobial activities of secondary metabolites and phylogenetic study of sponge endosymbiotic bacteria, bacillus sp. at agatti island, lakshadweep archipelago. | twenty-one species of sponges were recorded under the class of demospongiae and calcareous sponges of which 19 species were new to agatti reef. a total of 113 sponge endosymbiotic bacterial strains were isolated from twenty-one species of sponges and screened for antimicrobial activity. five bacterial strains of sponge endosymbiotic bacteria (seb) namely seb32, seb33, seb36, seb43 and seb51 showed antimicrobial activity against virulent marine fish pathogens such as vibrio alginolyticus, vibrio ... | 2016 | 28352539 |
| the immediate global responses of aliivibrio salmonicida to iron limitations. | iron is an essential micronutrient for all living organisms, and virulence and sequestration of iron in pathogenic bacteria are believed to be correlated. as a defence mechanism, potential hosts therefore keep the level of free iron inside the body to a minimum. in general, iron metabolism is well studied for some bacteria (mostly human or animal pathogens). however, this area is still under-investigated for a number of important bacterial pathogens. aliivibrio salmonicida is a fish pathogen, an ... | 2015 | 25649684 |
| cmc stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics. | in the present study silver nanoparticles were synthesized by reduction of agno3 using aqueous cmc solution, which acts as both reducing and capping agent. the formation of agno3 nanoparticles was observed visually by color change and these nanoparticles were characterized through uv-vis spectroscopy, ftir, xrd, sem, eds and afm. the ftir peaks observed to be ranging from 3300 to 605cm(-1). the afm image clearly showed the surface morphology of well dispersed nanoparticles. sem image illustrates ... | 2017 | 28024537 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods. | capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ... | 2016 | 27494415 |
| antibacterial, antiviral, and antifungal properties of wines and winery byproducts in relation to their flavonoid content. | grapes produce organic compounds that may be involved in the defense of the plants against invading phytopathogens. these metabolites include numerous phenolic compounds that are also active against human pathogens. grapes are used to produce a variety of wines, grape juices, and raisins. grape pomace, seeds, and skins, the remains of the grapes that are a byproduct of winemaking, also contain numerous bioactive compounds that differ from those found in grapes and wines. this overview surveys an ... | 2014 | 24945318 |
| quorum-sensing regulates biofilm formation in vibrio scophthalmi. | in a previous study, we demonstrated that vibrio scophthalmi, the most abundant vibrio species among the marine aerobic or facultatively anaerobic bacteria inhabiting the intestinal tract of healthy cultured turbot (scophthalmus maximus), contains at least two quorum-sensing circuits involving two types of signal molecules (a 3-hydroxy-dodecanoyl-homoserine lactone and the universal autoinducer 2 encoded by luxs). the purpose of this study was to investigate the functions regulated by these quor ... | 2012 | 23198796 |
| mechanism of anti-vibrio activity of marine probiotic strain bacillus pumilus h2, and characterization of the active substance. | vibriosis is a major epizootic disease that impacts free-living and farmed fish species worldwide. use of probiotics is a promising approach for prevention of vibrio infections in aquaculture. a probiotic anti-vibrio strain, bacillus pumilus h2, was characterized, and the mechanism of its effect was investigated. all 29 vibrio strains tested were growth-inhibited by h2. the anti-vibrio substance present in cell-free supernatant of h2 was purified and characterized by reversed-phase hplc. minimum ... | 2017 | 28097594 |
| a specific antimicrobial protein cap-1 from pseudomonas sp. isolated from the jellyfish cyanea capillata. | a bacterium strain, designated as cmf-2, was isolated from the jellyfish cyanea capillata and its culture supernatant exhibited a significant antimicrobial activity. the strain cmf-2 was identified as pseudomonas sp. based on the morphological, biochemical and physiological characteristics as well as 16s rrna sequence analysis. in this study, an antimicrobial protein, named as cap-1, was isolated from the culture of cmf-2 through ammonium sulfate precipitation and gel filtration chromatography. ... | 2016 | 26529191 |
| bacterial virulence analysis using brine shrimp as an infection model in relation to the importance of quorum sensing and proteases. | brine shrimp are aquatic crustaceans belonging to a genus of artemia. this organism is widely used for testing the toxicity of chemicals. in this study, brine shrimp were evaluated as an infection model organism to study bacterial virulence. artemia nauplii were infected with various pathogenic bacteria, such as vibrio vulnificus, pseudomonas aeruginosa, burkholderia vietnamiensis, staphylococcus aureus, and escherichia coli, and the susceptibility to these bacteria was investigated by counting ... | 2014 | 25420421 |
| dietary supplementation of bacillus subtilis and fructooligosaccharide enhance the growth, non-specific immunity of juvenile ovate pompano, trachinotus ovatus and its disease resistance against vibrio vulnificus. | a feeding trial was conducted to investigate the effects of dietary administration of probiotic bacillus subtilis and prebiotic fructooligosaccharide (fos) on growth performance, immune responses and disease resistance of juvenile ovate pompano, trachinotus ovatus. one thousand six hundred and twenty individuals (initial body weight: 10.32 ± 0.46 g, mean ± s.e) were fed nine practical diets according to a 3 × 3 factorial design: the basal diet as the control diet supplemented with three levels o ... | 2014 | 24614017 |
| characterization of antibiotic resistant and enzyme producing bacterial strains isolated from the arabian sea. | marine bacteria are known to produce many bioactive molecules and extracellular enzymes of commercial importance. we have investigated the bacterial diversity of the coastal area of karwar, karnataka state, india. among these bacterial isolates, five bacterial strains were selected and identified by their morphological, biochemical characteristics and phylogenetic analysis based on 16s rrna gene sequences. the identified bacterial isolates, bacillus toyonensis pntb1, lysinibacillus sphaericus pt ... | 2016 | 28330094 |
| [postoperative necrotizing fasciitis: a rare and fatal complication]. | postoperative parietal complications can be exceptionally severe and serious threatening vital prognosis. necrotizing fasciitis is a rare infection of the skin and deep subcutaneous tissues, spreading along fascia and adipose tissue. it is mainly caused by group a streptococcus (streptococcus pyogenes) but also by other bacteria such as vibrio vulnificus, clostridium perfringens or bacteroides fragilis. necrotizing fasciitis is a real surgical and medical emergency. we report, in this study, a v ... | 2016 | 27279950 |
| species differences in alternative substrate utilization by the antibacterial target undecaprenyl pyrophosphate synthase. | undecaprenyl pyrophosphate synthase (upps) is a critical enzyme required for the biosynthesis of polysaccharides essential for bacterial survival. in this report, we have tested the substrate selectivity of upps derived from the mammalian symbiont bacteroides fragilis, the human pathogen vibrio vulnificus, and the typically benign but opportunistic pathogen escherichia coli. an anthranilamide-containing substrate, 2-amideanilinogeranyl diphosphate (2aa-gpp), was an effective substrate for only t ... | 2014 | 25020247 |
| infections in alcoholic liver disease. | alcoholic individuals are at increased risk of infection in general, in part because of immune defects. in addition, associated situations, such as depressed mental status, increase risk to specific syndromes such as lung abscess related to depressed consciousness and aspiration. social factors related to hygiene and living situations are also linked to specific microorganisms, such as mycobacteria tuberculosis, bartonella quintana, vibrio vulnificus, and capnocytophaga canimorus.. | 2012 | 23101982 |
| induced disruption of the iron-regulatory hormone hepcidin inhibits acute inflammatory hypoferraemia. | withdrawal of iron from serum (hypoferraemia) is a conserved innate immune antimicrobial strategy that can withhold this critical nutrient from invading pathogens, impairing their growth. hepcidin (hamp1) is the master regulator of iron and its expression is induced by inflammation. mice lacking hamp1 from birth rapidly accumulate iron and are susceptible to infection by blood-dwelling siderophilic bacteria such as vibrio vulnificus. in order to study the innate immune role of hepcidin against a ... | 2016 | 27423740 |
| the infectious and noninfectious dermatological consequences of flooding: a field manual for the responding provider. | meteorological data show that disastrous floods are increasingly frequent and more severe in recent years, perhaps due to climatic changes such as global warming. during and after a flood disaster, traumatic injuries, communicable diseases, chemical exposures, malnutrition, decreased access to care, and even mental health disorders dramatically increase, and many of these have dermatological manifestations. numerous case reports document typical and atypical cutaneous infections, percutaneous tr ... | 2015 | 26159354 |
| bacterial zoonoses of fishes: a review and appraisal of evidence for linkages between fish and human infections. | human contact with and consumption of fishes presents hazards from a range of bacterial zoonotic infections. whereas many bacterial pathogens have been presented as fish-borne zoonoses on the basis of epidemiological and phenotypic evidence, genetic identity between fish and human isolates is not frequently examined or does not provide support for transmission between these hosts. in order to accurately assess the zoonotic risk from exposure to fishes in the context of aquaculture, wild fisherie ... | 2015 | 25466575 |
| evaluation of ice slurries as a control for postharvest growth of vibrio spp. in oysters and potential for filth contamination. | raw oyster consumption is the most common route of exposure for vibrio spp. infections in humans. vibriosis has been increasing steadily in the united states despite efforts to reduce the incidence of the disease. research has demonstrated that ice is effective in reducing postharvest vibrio spp. growth in oysters but has raised concerns of possible contamination of oyster meat by filth (as indicated by the presence of fecal coliform bacteria or clostridium perfringens). this study examined the ... | 2015 | 26197291 |
| disease-outcome trees, eq-5d scores, and estimated annual losses of quality-adjusted life years (qalys) for 14 foodborne pathogens in the united states. | measures of disease burden such as quality-adjusted life years (qalys) are increasingly important to risk-based food safety policy. they provide a means of comparing relative risk from diverse health outcomes. we present detailed disease-outcome trees and eq-5d scoring for 14 major foodborne pathogens representing over 95% of foodborne illnesses, hospitalizations, and deaths due to specified agents in the united states (campylobacter spp., clostridium perfringens, cryptosporidium parvum, cyclosp ... | 2014 | 24588853 |