| comparison of sds-page protein patterns with other typing methods for investigating the epidemiology of 'klebsiella aerogenes'. | twenty-four cultures comprising 20 clinical isolates of 'klebsiella aerogenes' from two hospitals, a reference strain of 'k. aerogenes' and the type strains of three other klebsiella species, were characterized by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) of whole-cell proteins. the protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into 12 protein types. comparison with esta ... | 1990 | 2189744 |
| beta-lactamases, beta-lactamase inhibitors, and skin and skin-structure infections. | beta-lactamases have been known since the early 1940s when they were recognized as a major mechanism of resistance in staphylococcus aureus. the synthesis of semisynthetic penicillins provided agents that overcame the resistance of staphylococci, but as gram-negative bacteria became increasingly important as the cause of infections, plasmid-mediated beta-lactamases were recognized in the enterobacteriaceae, haemophilus, and chromosomally mediated beta-lactamases in klebsiella, and bacteroides we ... | 1990 | 2189913 |
| therapeutic effects of cefpirome, a new cephalosporin, on various models of infections in mice and rats. | cefpirome (hr 810) is a new cephalosporin with a 2,3-cyclopentenopyridine group in the 3-position side chain. it was compared with other cephem antibiotics in protective and therapeutic effects on various experimental infections, systemic and local, in mice and rats. hr 810 had more potent protective effect than ceftazidime (caz), cefoperazone (cpz), and cefotaxime (ctx) on systemic infections induced by escherichia coli ec-31, staphylococcus aureus smith, and serratia marcescens sm-6 in mice. a ... | 1990 | 2190011 |
| [the problems of microbial indication of water quality]. | in three experimental series the reliability of contemporary microbial indicators has been verified. it was shown that about 30% colonies growing on the endomedium belongs not to family enterobacteriaceae. the share of the so-called faecal coliforms growing at 44 degrees c is 54.6%. in comparison with the initial cultivations at the second passage this part increasing at about 126%. in waters of various quality is the viability of e. coli the smallest. also on the mc conkey medium at 44 degrees ... | 1990 | 2190569 |
| infection and rheumatic diseases: a review (2). | | 1990 | 2190662 |
| laboratory criteria in the diagnostics of intestinal infections of staphylococcal etiology in children. | faecal samples were investigated from 948 children hospitalized with the diagnose of acute intestinal infection. the authors studied the isolation and properties of a broad spectrum of microorganisms from children's faeces: salmonella, shigella, e. coli, s. aureus, rotaviruses, klebsiella, morganella, proteus. as a result, laboratory criteria were formulated of diagnosing in children intestinal infection of staphylococcal etiology. | 1990 | 2191037 |
| the purification, characterization and role of the d-type cytochrome oxidase of klebsiella pneumoniae during nitrogen fixation. | klebsiella pneumoniae synthesized only b-type and d-type cytochromes under the wide range of growth conditions tested, and reaction with co revealed two potential oxidases. the o-type oxidase was produced only in the presence of o2 and appeared to be repressed by glucose. the d-type oxidase was, by contrast, produced only in the absence of measurable o2 (less than 1 microm), and was the only oxidase expressed in nitrogen-fixing conditions. it was extracted from the membrane, purified and shown t ... | 1990 | 2191076 |
| [the antilysozyme activity of bacteria in the genus klebsiella]. | the ability to inactivate lysozyme was found in representatives of three species of the genus klebsiella bacteria: k. pneumoniae (117 strains), k. rhinoscleromatis (104 strains), k. ozaenae (90 cultures). the test cultures displayed a different antilysozyme activity, inactivating from 2 to 30 micrograms/ml of the enzyme. taking into account the lysozyme role in the immunity and chitin synthesis processes in the organism of insects, the inactivation of the enzyme by klebsiella may be considered a ... | 1990 | 2191198 |
| [etiological factors in secondary bacterial infections from the authors' own clinical material]. | an analysis of etiological factors of secondary bacterial infections was carried out in 64 patients with pneumonias. in most of the patients gram negative rods were cultured. most often the pneumonias were caused by klebsiella pneumoniae and escherichia coli. a single causative agent was found in the majority of the patients (60.7%), less frequently two, least three. | 1990 | 2191275 |
| ornithine decarboxylating strains of klebsiella pneumoniae demonstrated by dna-dna hybridization. | genotypic relatedness was assessed to clarify the taxonomic position of strains phenotypically behaving like k. pneumoniae, but for the ornithine reaction. using dna-dna hybridization it could be shown that 25 non-motile ornithine decarboxylating strains showed high genotypic relatedness to the type strain of k. pneumoniae. thus, it is proposed that they be considered as ornithine decarboxylating strains of the species k. pneumoniae. the api 20e system was used for phenotypic characterization, b ... | 1990 | 2191695 |
| kinetics and specificity of alginate lyases: part i, a case study. | purified preparations of alginate lyase from klebsiella aerogenes and haliotis sp. were investigated for activity and degradation patterns with alginate and alginate fragments having different compositions and sequences. with fragments approaching homopolymers of guluronate and mannuronate, michaelis-menten kinetics were obeyed and kinetic parameters could be obtained. degradation of alginates containing all four possible linkages in various proportions, followed by isolation of the fragments an ... | 1990 | 2191774 |
| characterisation of a unique ceftazidime-hydrolysing beta-lactamase, tem-e2. | a strain of klebsiella oxytoca, originally isolated in liverpool in 1982, has been found to produce a novel transferable beta-lactamase, tem-e2. this enzyme confers resistance to ceftazidime and focused as a doublet band with an iso-electric point (pi) of 5.3. the strain also produced the tem-1 beta-lactamase. both tem-1 and tem-e2 beta-lactamases were encoded by a transferable 103 kb plasmid; these two enzymes also had similar molecular weights, were inhibited by clavulanic acid, and hydrolysed ... | 1990 | 2192063 |
| [primary pyomyositis in mild climates. presentation of 2 new cases]. | pyomyositis is an acute bacterial infection which affects striated muscles. it is a relatively rare process in mild climates. staphylococcus aureus is responsible for 90-95% of cases. klebsiella pneumoniae pyomyositis is extremely rare with only one other case reported in a mild climate. two new cases of pyomyositis are described one caused by k. pneumoniae, increasing thus the etiology spectrum in our country, and the other caused by s. aureus ending in fatality, with two focus of pyomyositis ( ... | 1990 | 2192408 |
| [cytobacteriologic examination of the urine. execution. interpretation]. | bacteriological examination of urine is sufficient to assert the presence of a urinary tract infection (uti). specimens must be collected under strictly aseptic conditions. the midstream jet method is the best, but when it is not feasible bladder puncture, sterile pouches or catheterization may be used. the urine obtained must be correctly preserved and transported. in the practitioner's office, uti can be detected by reagent strips or agar-coated plates. in the laboratory, direct examination is ... | 1990 | 2193363 |
| in vitro antibacterial activity and interactions with beta-lactamases and penicillin-binding proteins of the new monocarbam antibiotic u-78608. | u-78608, a new monocarbam antibiotic, was evaluated for in vitro activity against 312 clinical isolates of aerobic and anaerobic bacteria and subjected to several in vitro biochemical tests characterizing its interactions with beta-lactamases and penicillin-binding proteins (pbps). the antibacterial activity of the compound was compared directly with those of sq 83,360 (pirazmonam) and aztreonam. u-78608, sq 83,360, and aztreonam had generally poor activity against gram-positive aerobic bacteria ... | 1990 | 2193625 |
| [hospital and district differences in susceptibilities to antibiotics of main clinical isolates]. | a nationwide survey of susceptibilities of staphylococcus aureus, escherichia coli, klebsiella sp. and proteus mirabilis initiated in 1980 was continued in 1986. this report documents differences in susceptibilities of clinical isolates of the above microorganisms to ampicillin (abpc), cefazolin (cez), cefmetazole (cmz) and gentamicin (gm) among general hospitals throughout japan. clinical isolates of each species of microorganisms which were collected in 24 hospitals made up each study group an ... | 1990 | 2194057 |
| evidence for the ability of l10 ribosomal proteins of salmonella typhimurium and klebsiella pneumoniae to regulate rpljl gene expression in escherichia coli. | genes rplj, coding for ribosomal protein l10 of salmonella typhimurium and klebsiella pneumoniae, have been cloned on puc plasmid. the resultant multicopy recombinant plasmids were detrimental for the growth of normal jm101 e. coli host cells and harmless for the mutant jf3029 host. this negative effect is the evidence for the ability of heterologous l10 proteins to regulate expression of rpljl genes in e. coli. nucleotide sequence was determined completely for s. typhimurium rpljl' dna portion ... | 1990 | 2194828 |
| intramammary challenge of the bovine mammary gland with coliform bacteria during early involution. | isolates of escherichia coli (n = 12), klebsiella pneumoniae (n = 20), and klebsiella oxytoca (n = 10) were used to challenge involuting mammary glands at 7 d of the dry period. bacteria were selected for challenge on the basis of their ability to grow in a pooled source of dry cow secretion obtained at 21 d of involution. challenge bacteria were classified as highly adapted (in vitro growth greater than 7 cfu log10/ml) or poorly adapted (growth less than 2 cfu log10/ml) for growth in dry cow se ... | 1990 | 2195077 |
| [in vitro antibacterial activity of ru 51746 (sodium salt of cefpodoxime). results of a multicenter study]. | cefpodoxime proxetil, a new oral cephalosporin, is the prodrug ester of cefpodoxime. minimal inhibitory concentrations (mic) of ru 51746 (sodium salt of cefpodoxime: cpd) were evaluated by agar dilution for 1 696 bacterial strains isolated in 5 hospitals. for enterobacteriaceae, mic 50 and 90% were respectively (micrograms/ml): (1) naturally non bêtalactamase producing species: e. coli, shigella and salmonella 0.25-0.5; p. mirabilis 0.06-0.12. (ii) chromosomal penicillinase producing species: kl ... | 1990 | 2195445 |
| [comparative antibacterial activity of ceftibuten (sh 39 720) against 150 k. pneumoniae strains producing different beta-lactamases]. | comparative antibacterial activity of ceftibuten (sh 39 720) on 150 k. pneumoniae strains producing different beta-lactamases. mics of ceftibuten, cefotaxime + clavulanic acid (10 mg/l), ceftazidime and azthreonam were determined for 150 clinical isolates of k. pneumoniae: 15 "wild" type producing only the shv-1 type beta-lactamase, 15 tem-1 and 120 producing new "extended spectrum" beta-lactamases (48 ctx-1, 9 shv-2, 4 shv-3, 35 shv-4, 24 shv-5). against shv-1 and tem-1 strains the bacteriostat ... | 1990 | 2195446 |
| [in vitro activity of 4 fluoroquinolones against klebsiella pneumoniae. distribution according to phenotype resistance to aminoglycosides or beta-lactams]. | minimal inhibitory concentrations (mic) of pefloxacin (pef), norfloxacin (nor), ofloxacin (ofl) and ciprofloxacin (cip) are evaluated by agar dilution against 100 klebsiella pneumoniae strains isolated in hospital. mic 50 and 90%, micrograms/ml, are respectively: cip (0.25/8), ofl (1/16), nor (1/32), pef (2/64). we determined the phenotypes pef/nor/ofl/cip by taking into account the critic concentrations of the french committee for antibiogram. the results are: ssss = 45%, ri/ri/ri/ri = 20%, ri/ ... | 1990 | 2195447 |
| [frequency and distribution of beta-lactamases in 1792 strains of klebsiella pneumoniae in france between 1985 and 1988]. | in october 1985, 1987 and 1988, all the clinical isolates of k. pneumoniae (respectively 530, 654, 590 strains) were collected in 20 hospitals. the beta-lactamases were identified by analytical isoelectrofocusing and by substrate and inhibition profiles. 76 to 81% of the strains produced only one beta-lactamase: shv-1 type, pi 7.7 (61 to 65%) or pi 7.1 (14%). the tem-1 betalactamase (pi 5.4) was produced in 1985 by 21% of the strains, 9% in 1987, and 11% in 1988: tem-2, pi 5.6 by 2% in 1985-87-8 ... | 1990 | 2195456 |
| [enzymatic resistance to cefotaxime in 56 strains of klebsiella spp., escherichia coli and salmonella spp. at a tunisian hospital (1984-1988)]. | the enzymatic and plasmid-encoded resistance towards oxyimino-beta-lactams has been recently reported as related to the production of an extended-spectrum beta-lactamase (e.g. shv-2, ctx-1 or tem-3), in particular in our hospital since 1984. the prevalence of that resistance has been examined from january 1984 to december 1988 in function of specimen, unit and type of enzyme among 8,421 isolates of klebsiella spp., e. coli, salmonella spp. each isolate showing a diameter of inhibition zone size ... | 1990 | 2195457 |
| [a clinical trial of pefloxacin (abaktal) in the treatment of acute pyelonephritis]. | the therapeutic effect of pefloxacin in the treatment of acute pyelonephritis was examined in a randomized clinical trial. the patients in the control group have been treated by gentamycin. with a success rate of 80% in the treatment of acute pyelonephritis caused by various bacteria (e. coli, pseudomonas, klebsiella, staphylococcus aureus, proteus mirab.), a side frequency of side effects and a good compliance pefloxacin is recommended as an effective antimicrobiell drug. | 1990 | 2195800 |
| potentiation of aminoglycoside inhibition and reduction of capsular polysaccharide production in klebsiella pneumoniae by sodium salicylate. | this study determined the effects of sodium salicylate combined with several aminoglycoside antibiotics on the growth and capsular polysaccharide (cps) production of klebsiella pneumoniae. salicylate significantly enhanced in inhibitory effect of all aminoglycoside antibiotics against all bacterial strains tested. the production of cps was decreased by 62-86% when 2.5 mm salicylate was used. amikacin combined with salicylate reduced cps only slightly more than salicylate alone. the chelating age ... | 1990 | 2196259 |
| central nervous system infection after endoscopic injection sclerotherapy. | central nervous system (cns) infection is a rare complication of endoscopic injection sclerotherapy (eis) for esophageal varices. we report two patients, one of whom developed a solitary brain abscess, and the other, acute meningitis, after eis. they presented with high fever initially, and then with changes in mental status. in the case of the solitary brain abscess, the csf revealed evidence of infection, and ct scan disclosed a brain abscess in the left temporo-parieto-occipital region. this ... | 1990 | 2196787 |
| isolation of fluoroquinolone-resistant escherichia coli and klebsiella pneumoniae from an infected hickman catheter. | escherichia coli and klebsiella pneumoniae resistant to fluoroquinolones were isolated from an infected hickman catheter in a 43-year-old diabetic patient who had previously been treated with a 24-day course of ciprofloxacin (200 mg/12 h i.v.). mics and mbcs of nalidixic acid, norfloxacin, ciprofloxacin, ofloxacin, pefloxacin and fleroxacin were determined for the strains using the methodology recommended by the nccls. both strains were resistant to all the quinolones tested. since long-term tre ... | 1990 | 2197092 |
| review of available trials of selective decontamination of the digestive tract (sdd). | eighteen studies on selective decontamination of the digestive tract (sdd) have been published up to now. a statistically significant reduction of infection rate was found in fourteen out of the fifteen controlled studies. although all the studies were designed to evaluate infection-related morbidity as the end point, ten centres have reported fatality rates. six centres out of the ten showed a statistically significant reduction in mortality in patients receiving sdd versus control. a recent fr ... | 1990 | 2197222 |
| [pharmacokinetic, bacteriological and clinical studies on aztreonam in neonates]. | pharmacokinetic, bacteriological and clinical studies on aztreonam (azt) were performed in neonates. the results obtained are summarized as follows. 1. plasma levels and urinary excretion of azt were determined in 18 neonates with ages between 1 and 30 days (gestation periods were 36 to 40 weeks and birth weights were 1,890 to 4,300 g) and in 2 infants with 54 and 60 days of age (gestation periods were 36 and 40 weeks, and birth weights were 2,300 and 3,300 g, respectively) upon one-shot intrave ... | 1990 | 2197467 |
| complementation of escherichia coli sigma 54 (ntra)-dependent formate hydrogenlyase activity by a cloned thiobacillus ferrooxidans ntra gene. | the ntra gene of thiobacillus ferrooxidans was cloned by complementation of an escherichia coli ntra mutant that was unable to produce gas via the sigma 54 (ntra)-dependent formate hydrogenlyase pathway. analysis of the dna sequence showed that the t. ferrooxidans ntra gene coded for a protein of 475 amino acids (calculated mr, 52,972). the t. ferrooxidans ntra protein had 49, 44, 33, and 18% amino acid similarity with the ntra proteins of klebsiella pneumoniae, azotobacter vinelandii, rhizobium ... | 1990 | 2198257 |
| genetic evidence that narl function is not required for nitrate regulation of nitrate assimilation in klebsiella pneumoniae m5al. | we cloned the narl gene, required for nitrate induction of respiratory nitrate reductase synthesis, from klebsiella pneumoniae. the e. coli narl gene product shares sequence similarity with the response regulator proteins of two-component regulatory systems. we found that narl(+)-containing plasmids restored nitrate regulation of anaerobic respiratory gene expression in appropriate escherichia coli hosts. the k. pneumoniae narl region encoded a protein whose migration in laemmli gels was indisti ... | 1990 | 2198261 |
| hepatic cyst infection in autosomal dominant polycystic kidney disease. | to characterize the syndrome of hepatic cyst infection in autosomal dominant polycystic kidney disease (adpkd) and to review its diagnosis and management, we retrospectively studied five such cases in patients from our institution and nine detailed case reports from the literature. the clinical manifestations were an acute (58%) or subacute (42%) febrile illness, typically associated with tenderness in the right upper quadrant, leukocytosis, a very high erythrocyte sedimentation rate, but minor ... | 1990 | 2198396 |
| production of shiga-like toxin among escherichia coli strains and other bacteria isolated from diarrhea in são paulo, brazil. | an elevated level of shiga-like toxin i (slt-i) production was found in 1 of 466 escherichia coli strains studied. among the 34 sonic lysates obtained from classical enteropathogenic e. coli, 5 produced slt-i. the aeromonas, citrobacter, edwardsiella, enterobacter, klebsiella, proteus, providencia, pseudomonas, salmonella, serratia, shigella, yersinia, and vibrio strains also studied were not slt producers, except for a shigella dysenteriae type 1 strain. although slt-i-producing e. coli strains ... | 1990 | 2199511 |
| the effect of fresh human serum on ofloxacin bactericidal activity. | we have studied the possibility of an increase in ofloxacin bactericidal activity when it is combined with fresh human serum. the tested strains were 10 clinical isolates of klebsiella pneumoniae. from among our strains 5 were susceptible to serum bactericidal activity and 5 were found to be resistant. we selected two serum concentrations (15 and 35%) to test against susceptible strains and two (55 and 75%) to test against resistant strains in combination with the minimum inhibitory concentratio ... | 1990 | 2199626 |
| [in vitro susceptibility of escherichia coli and klebsiella sp. to antibiotics]. | a nationwide susceptibility survey of clinical isolates of escherichia coli and klebsiella pneumoniae initiated in 1980 was continued for the 8th consecutive year. a total of 4,421 strains of e. coli and 2,825 strains of k. pneumoniae isolated mainly from urine, sputum and pus, were obtained from 69 hospitals throughout japan during the 2 years (1986-1987). mics were determined using the agar plate dilution method (mueller-hinton agar, bbl) with inoculation of 10(8)cfu/ml bacteria. antibiotics t ... | 1990 | 2199690 |
| [the immunomodulating properties of a glucomacropeptide from whey. i. the stimulation of resistance in mice]. | the immunomodulating effect on mice of three products glucomacropeptides obtained from whey with different carbohydrate and peptide components has been studied. after the preliminary application of the preparations at several doses the protective effect in infections with salmonella typhimurium and klebsiella pneumoniae increases to different extent. at lower doses fraction 3 increases in vitro the mitogen-induced proliferation of the thymus cells. the phagocytosis of the polymorphonuclear cells ... | 1990 | 2200242 |
| [microbiological control of drug resistance of causative agents in suppurative-septic infections in newborn infants]. | resistance of the main causative agents of purulent septic infections such as pneumonia, meningitis, sepsis, etc. to 11 chemotherapeutic drugs was studied. the pathogens were isolated from autopsies of 203 newborns who died within 1981 to 1987. among 2978 isolates belonging to enterobacteriaceae (2466 strains) and pseudomonadaceae (512 strains) which constituted 88% of all the isolates, strains with multiple resistance predominated: 90% of the strains resistant to 4 or more antibiotics, 77% of t ... | 1990 | 2200371 |
| phenotypic properties of klebsiella pneumoniae and k. oxytoca isolated from different sources. | 474 klebsiella pneumoniae and k. oxytoca strains isolated from different sources (human clinical material, feces of healthy subjects, sewage) were investigated for phenotypic properties. characteristics analyzed were cultural activities, antimicrobial susceptibilities and capsule types. comparison of both species revealed differences in adonitol fermentation and resistance to tetracycline, nalidixic and pipemidic acid. capsule types 2, 7 and 33 were frequently found in k. pneumoniae, but not in ... | 1990 | 2200423 |
| klebsiella pulmonary infections: occurrence at one medical center and review. | klebsiella pneumoniae is the species of aerobic gram-negative bacteria most commonly recognized as a cause of community-acquired pneumonia. the vast majority of articles concerning the epidemiology, frequency, and clinical and radiographic features of community-acquired pneumonias caused by this organism are over 20 years old. experience with community-acquired pneumonias due to k. pneumoniae at one medical center over the last 20 years is reported and compared with the previously published lite ... | 1990 | 2201068 |
| [the pathogenicity factors of the opportunistic bacteria that cause diarrhea]. | | 1990 | 2201150 |
| [the reactogenicity and immunological efficacy of a klebsiella vaccine in donors]. | klebsiella vaccine, when injected subcutaneously to donors, proved to be faintly reactogenic and safe. the injection of the vaccine had no effect on changes in the morphological composition of peripheral blood and on liver function. in persons with the initially low content of igg an increase in this characteristic was observed after immunization. no changes in the synthesis of ige occurred in healthy donors under the influence of immunization. the vaccine was shown to be immunogenic when introd ... | 1990 | 2201154 |
| nucleotide sequence of shv-2 beta-lactamase gene. | the nucleotide sequence of plasmid-mediated beta-lactamase shv-2 from salmonella typhimurium (shv-2pht1) was determined. the gene was very similar to chromosomally encoded beta-lactamase len-1 of klebsiella pneumoniae. compared with the sequence of the escherichia coli shv-2 enzyme (shv-2e.coli) obtained by protein sequencing, the deduced amino acid sequence of shv-2pht1 differed by three amino acid substitutions. | 1990 | 2201259 |
| interleukin-6 gene expression and production induced in human monocytes by membrane proteoglycans from klebsiella pneumoniae. | the present study was designed to investigate the effect of membrane proteoglycans (mpg) from klebsiella pneumoniae on il-6 production by human peripheral blood monocytes. exposure in vitro to mpg induced release of il-6 activity from human monocytes, as assessed by the 7td1 hybridoma assay. mpg-induced hybridoma growth factor activity was blocked by anti-il-6 antibodies. mpg induced expression in human monocytes of il-6 mrna transcripts as assessed by northern blot analysis. induction of il-6 i ... | 1990 | 2202691 |
| characterization of enterobacteria by esterase specific-activity profiles. | the spectrum of specific activities and the electrophoretic mobilities of esterases produced by 550 strains of enterobacteriaceae belonging to 36 species and subclassified into six groups (group 1, escherichia coli, shigella and escherichia hermanii; group 2, genus salmonella and genus citrobacter; group 3, genus klebsiella and genus enterobacter; group 4, genus serratia and serratia fonticola; group 5, genus proteus, genus providencia and genus morganella; and group 6, genus yersinia) were anal ... | 1990 | 2202780 |
| inhibitory effect of ca2+ on formation of mg2(+)-mediated two-dimensional hexagonal lattice structure by an r-form lipopolysaccharide from klebsiella pneumoniae. | when the r-form lipopolysaccharide (lps) from klebsiella pneumoniae strain len-111 (o3-:k1-), from which cationic material had been removed by electrodialysis, was suspended in 50 mm tris buffer at ph 8.5 containing 0.1 mm or higher concentrations of mgcl2, it formed an ordered two-dimensional hexagonal lattice structure and its center-to-center distance (lattice constant) depended upon the concentration of mgcl2 and reached the shortest value (14 nm) at 10 mm. in contrast, in the presence of 0. ... | 1990 | 2202890 |
| failure of ceftazidime-amikacin therapy for bacteremia and meningitis due to klebsiella pneumoniae producing an extended-spectrum beta-lactamase. | a multiple trauma patient failed treatment with ceftazidime and amikacin for bacteremia and meningitis due to a klebsiella pneumoniae strain that produced a novel, plasmid-mediated beta-lactamase. both pre- and posttreatment isolates were resistant to ceftazidime (mic, greater than or equal to 64 micrograms/ml) and various penicillins but not to other expanded-spectrum cephalosporins. the beta-lactamase had a pi of 5.25 and was encoded on a conjugal plasmid of approximately 150 kilobases. dna hy ... | 1990 | 2203306 |
| r plasmids from clinical and environmental klebsiella pneumoniae. | a total of 200 strains of k. pneumoniae, 150 from clinical and 50 from hospital environmental source were studied for antibiotic resistance pattern. the strains from clinical source showed higher incidence of drug resistance as compared to environmental strains. 50 clinical and 32 environmental multidrug resistant strains were tested for r plasmids. the biogroup 1 showed higher incidence of autotransferring and non autotransferring r plasmids. | 1990 | 2203678 |
| nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of pseudomonas putida. | the hutc gene of pseudomonas putida encodes a repressor which, in combination with the inducer urocanate, regulates expression of the five structural genes necessary for conversion of histidine to glutamate, ammonia, and formate. the nucleotide sequence of the hutc region was determined and found to contain two open reading frames which overlapped by one nucleotide. the first open reading frame (orf1) appeared to encode a 27,648-dalton protein of 248 amino acids whose sequence strongly resembled ... | 1990 | 2203753 |
| nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of klebsiella aerogenes. | the hutc gene of klebsiella aerogenes encodes a repressor that regulates expression of the histidine utilization (hut) operons. the dna sequence of a region known to contain hutc was determined and shown to contain two long rightward-reading open reading frames (orfs). one of these orfs was identified as the 3' portion of the hutg gene. the other orf was the hutc gene. the repressor predicted from the hutc sequence contained a helix-turn-helix motif strongly similar to that seen in other dna-bin ... | 1990 | 2203754 |
| genes required for formation of the apomofe protein of klebsiella pneumoniae nitrogenase in escherichia coli. | a binary plasmid system was used to produce nitrogenase components in escherichia coli and subsequently to define a minimum set of nitrogen fixation (nif) genes required for the production of the iron-molybdenum cofactor (femoco) reactivatable apomolybdenum-iron (apomofe) protein of nitrogenase. the active mofe protein is an alpha 2 beta 2 tetramer containing two femoco clusters and 4 fe4s4 p centers (for review see, orme-johnson, w.h. (1985) annu. rev. biophys. biophys. chem. 14, 419-459). the ... | 1990 | 2203791 |
| diastereomer-dependent substrate reduction properties of a dinitrogenase containing 1-fluorohomocitrate in the iron-molybdenum cofactor. | in vitro synthesis of the iron-molybdenum cofactor (femo-co) of dinitrogenase using homocitrate and its analogs allows the formation of modified forms of femo-co that show altered substrate specificities (n2, acetylene, cyanide, or proton reduction) of nitrogenase [reduced ferredoxin:dinitrogen oxidoreductase (atp-hydrolyzing), ec 1.18.6.1]. the (1r,2s)-threo- and (1s,2s)-erythro-fluorinated diastereomers of homocitrate have been incorporated in vitro into dinitrogenase in place of homocitrate. ... | 1990 | 2204057 |
| [a case of klebsiella sepsis with an atypical course]. | | 1990 | 2205048 |
| [substrate specificity of restriction endonuclease kpn378i]. | | 1990 | 2205206 |
| citrate lyase from klebsiella pneumoniae. the complete primary structure of the acyl lyase subunit. | the primary structure of the beta-subunit (acyl lyase subunit) of citrate lyase from klebsiella pneumoniae (atcc 13,882) was determined with protein chemical methods. the polypeptide chain consists of 289 amino acid residues and has a molecular mass of 31,352 da. the two half-cystine residues of the subunit are present as cysteines and not involved in disulfide bridges. the sequence shows no homology to known sequences of proteins or nucleic acids and reads (sequence; see text) | 1990 | 2205500 |
| alternative nitrogenase. | recently, it has been demonstrated that both a. vinelandii and a. chroococcum have the ability to synthesize several different nitrogen-fixing enzymes. both species can produce a mo- or v-containing nitrogenase while a. vinelandii can also generate an all-fe form of the enzyme. regardless of the source or form of the enzyme, all nitrogenases are composed of two separable proteins, called components 1 and 2, where component 2 is a highly conserved dimer containing a single [4fe-4s] cluster. the m ... | 1990 | 2206026 |
| construction of a new catabolic pathway for d-fructose in escherichia coli k12 using an l-sorbose-specific enzyme from klebsiella pneumoniae. | starting with a fruk (formerly fpk) mutant of escherichia coli k12 lacking d-fructose-1-phosphate kinase (e.c. 2.7.1.3.), fructose positive derivatives were isolated after introduction of the cloned gene sore from klebsiella pneumoniae coding for an l-sorbose-1-phosphate reductase. the new pathway was shwon to proceed from d-fructose via d-fructose-1-phosphate and d-mannitol-1-phosphate to d-fructose 6-phosphate. it involves a transport system and enzymes encoded in the fru and the mtl operons f ... | 1990 | 2206103 |
| the integration host factor stimulates interaction of rna polymerase with nifa, the transcriptional activator for nitrogen fixation operons. | the regulatory protein nifa activates transcription of nitrogen fixation (nif) operons by the sigma 54 holoenzyme form of rna polymerase. nifa from klebsiella pneumoniae activates transcription from the nifh promoter in vitro; in addition, the integration host factor, ihf, binds between the nifh promoter and an upstream binding site for nifa. we demonstrate here that ihf greatly stimulates nifa-mediated activation of nifh transcription in vitro and thus that the two factors are functionally syne ... | 1990 | 2208275 |
| in vitro and in vivo effects of subinhibitory concentrations of clindamycin on experimental klebsiella pneumoniae sepsis. | we studied the effect of subinhibitory doses of clindamycin on the course of experimental klebsiella pneumoniae sepsis. wistar rats were injected intraperitoneally with an inoculum containing 5 x 10(6) colony-forming units of k. pneumoniae resistant to clindamycin (minimum inhibitory concentration greater than 128 micrograms/ml) and then distributed to receive clindamycin 10 mg/kg/day or placebo for 10 days. all animals were bacteremic at 3 h. when the magnitude of bacteremia was compared, no di ... | 1990 | 2209167 |
| infection with bacterial enteropathogens in swedish travellers to south-east asia--a prospective study. | infection with potential bacterial enteropathogens was studied prospectively in 94 swedish travellers. three faecal samples were collected, regardless of diarrhoeal symptoms, after each of three 1-week stays in singapore, hong kong and japan. the specimens were analyzed for salmonella, shigella, yersinia, campylobacter and different enterotoxin-producing bacteria. a potential enteropathogen was identified in 30% (28/94) of the participants, i.e. in 26% of the healthy and in 39% of the travellers ... | 1990 | 2209737 |
| separation of plasmid-mediated extended spectrum beta-lactamases by fast protein liquid chromatography (fplc system). | we have devised a reliable procedure for the separation of three beta-lactamases of isoelectric focusing points (pi), 5.4, 6.5, and 7.9 by fast protein liquid chromatography (fplc system). all of these enzymes were transferable and originated from a ceftazidime and cefotaxime resistant klebsiella pneumoniae isolated in bombay, india. the complete separation of the enzymes, achievable by this method, allowed each of the different individual beta-lactamases to be characterized biochemically. this ... | 1990 | 2210330 |
| lytic effect of vibrio cholerae elastase on gram-positive and -negative bacteria. | elastase of vibrio cholerae caused the lysis of freshly grown cells of gram-negative (pseudomonas aeruginosa, proteus vulgaris, salmonella paratyphi a and klebsiella pneumoniae) bacteria. gram-positive (staphylococcus aureus and s. epidermidis) organisms were resistant to this enzyme. heat killed and lyophilized gram-positive and -negative bacteria (except s. aureus and s. epidermidis) showed higher sensitivity to elastase. both gram-negative and -positive bacteria were lyzed maximally by elasta ... | 1990 | 2210489 |
| effect of ru 41740 on autologous hemopoietic reconstitution of sublethally irradiated mice. | ru 41740, a glyco-protein extract of klebsiellae pneumoniae 01 k2 strain, is known to have immunomodulator activities. it acts on macrophages as well as on b and t-cells, and enhances their cytokine production, particularly interleukin 1 (il-1). the fact that cytokines have an effect on hemopoiesis led us to suspect an effect of ru 41740 on hemopoietic reconstitution. in this study, a model of autologous reconstitution of a hemopoietic system after sublethal irradiation in mice was used. c57 bl/ ... | 1990 | 2210912 |
| antibacterial activity of cefpodoxime proxetil in a pharmacokinetic in-vitro model. | the antibacterial activity of cefpodoxime proxetil was studied in an in-vitro model simulating doses of 100, 200 and 400 mg. strains of klebsiella spp. proteus mirabilis, escherichia coli, streptococcus pyogenes, and haemophilus influenzae were effectively reduced by a dose of 200 mg. while for esch. coli no dose-activity relationship was observed--the maximal effect was achieved with a simulated dose of 100 mg--staphylococcus aureus could be reduced effectively only by a simulated dose of 400 m ... | 1990 | 2211449 |
| sequence and analysis of the rpon sigma factor gene of rhizobium sp. strain ngr234, a primary coregulator of symbiosis. | we report the nucleotide sequence of the rpon gene from broad-host-range rhizobium sp. strain ngr234 and analyze the encoded rpon protein, a sigma factor. comparative analysis of the deduced amino acid sequence of rpon from ngr234 with sequences from other gram-negative bacteria identified a perfectly conserved rpon box unique to rpon sigma factors. symbiotic regulatory phenotypes were defined for a site-directed internal deletion within the coding sequence of the rpon gene of rhizobium strain n ... | 1990 | 2211497 |
| sequence of the klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation. | a 4.8-kilobase-pair region of cloned dna encoding the genes of the klebsiella aerogenes urease operon has been sequenced. six closely spaced open reading frames were found: urea (encoding a peptide of 11.1 kilodaltons [kda]), ureb (11.7-kda peptide), urec (60.3-kda peptide), uree (17.6-kda peptide), uref (25.2-kda peptide), and ureg (21.9-kda peptide). immediately after the ureg gene is a putative rho-dependent transcription terminator. the three subunits of the nickel-containing enzyme are enco ... | 1990 | 2211515 |
| synthesis of the iron-molybdenum cofactor of nitrogenase is inhibited by a low-molecular-weight metabolite of klebsiella pneumoniae. | the in vitro synthesis of the iron-molybdenum cofactor nitrogenase was inhibited by a low-molecular-weight factor. this inhibitory factor was present in the membrane extracts of wild-type and nif mutant strains of klebsiella pneumoniae that were grown under conditions that either repressed or derepressed nitrogenase expression. in vitro, the inhibition was specific for the nifb protein. addition of this factor to k. pneumoniae cells at various times during nif derepression decreased nitrogenase ... | 1990 | 2211526 |
| conversion of 5-s-methyl-5-thio-d-ribose to methionine in klebsiella pneumoniae. stable isotope incorporation studies of the terminal enzymatic reactions in the pathway. | extracts of klebsiella pneumoniae convert 5-s-methyl-5-thio-d-ribose (methylthioribose) to methionine and formate. to probe the terminal steps of this biotransformation, [1-13c]methylthioribose has been synthesized and its metabolism examined. when supplemented with mg2+, atp, l-glutamine, and dioxygen, cell-free extracts of k. pneumoniae converted 50% of the [1-13c]methylthioribose to [13c]formate. the formation of [13c]formate was established by 13c and 1h nmr spectroscopy studies of the purif ... | 1990 | 2211600 |
| autoantibodies to the hla-b27 sequence cross-react with the hypothetical peptide from the arthritis-associated shigella plasmid. | we previously reported elevated serum antibody levels to a peptide representing the hla-b27 polymorphic region (b27 peptide) in hla-b27(+) ankylosing spondylitis (as) patients. a plasmid (phs-2) isolated from arthritogenic shigella flexneri strains had been shown to encode an amino acid sequence homologous to hla-b27. rabbit antibody to this sequence (phs-2 peptide) strongly cross-reacted with b27 peptide and, to a much lesser extent, with klebsiella nitrogenase peptide. serum antibody levels to ... | 1990 | 2212008 |
| use of urea filter paper disc to detect urease activity in enterobacteriaceae by multipoint replication techniques. | a new method of detecting urease activity in enterobacteriaceae was developed. an 8.5 cm filter paper disc impregnated with 20% urea and 0.5% bromocresol purple was placed on the surface of a glucose fermentation plate after inoculation with a multipoint replicator and overnight incubation. this method was compared with the commercially prepared mast urea agar (multipoint) and fuscoe's urea plate medium. a total of 240 routine isolates of enterobacteriaceae were tested for urease activity using ... | 1990 | 2212071 |
| [pulmonary abscesses in material from tuberculosis and lung disease clinics of the academy of medicine in gdansk]. | 47 patients with pulmonary abscesses were analysed. the type of bacteria cultured from the sputum, type of chemotherapy and clinical outcome were assessed. most of the patients (34) were already treated prior to admission to the department. most often with doxycycline and gentamycin. the following organisms were cultured: streptococcus viridans, escherichia coli, staphylococcus aureus and klebsiella pneumoniae. antibiotic therapy was always instituted according to the antibiogram. most often ami ... | 1990 | 2216912 |
| overview of experience with ofloxacin in respiratory tract infection. | ofloxacin is highly active against common respiratory pathogens including haemophilus influenzae and branhamella catarrhalis and has clinically applicable activity against streptococcus pneumoniae, staphylococcus aureus and pseudomonas aeruginosa. sputum, lung tissue and bronchial mucosal concentrations of ofloxacin equal or, in most cases significantly exceed the mics of such pathogens. these in vitro attributes are reflected in the results of the worldwide ofloxacin clinical trial program whic ... | 1990 | 2218424 |
| klebsiella pneumoniae infection in chinchillas. | | 1990 | 2219635 |
| cloning of shv-2, ohio-1, and oxa-6 beta-lactamases and cloning and sequencing of shv-1 beta-lactamase. | molecular cloning of dna fragments permitted the isolation of structural genes coding for shv-1, shv-2, ohio-1, and oxa-6 beta-lactamases. dna probes were constructed for shv-1, and under conditions of high stringency, hybridization was observed only between shv-1 and shv-2. oligonucleotide typing with a 15-mer shv-1 probe was capable of discriminating between shv-1 and shv-2 but not ohio-1. the nucleotide sequence of the shv-1 beta-lactamase gene from plasmid r974 has been determined. the struc ... | 1990 | 2221867 |
| [septicemia in children with malignant disease]. | severe infection is a major cause of morbidity and mortality in patients with malignancy. in this study, 34 episodes of septicemia occurred in 1,468 childhood patients with malignancy who admitted and were treated at national sapporo hospital between 1979 and 1988. the occurrence of septicemia and its mortality rate were higher in malignant hematologic disease than in malignant solid tumor. most cases of septicemia occurred in relapse. the most frequent organism causing septicemia were klebsiell ... | 1990 | 2221930 |
| hemolysin as a marker for serratia. | all serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. dna fragments of the two hemolysin genes hybridized with the chromosomal dna of s. marcescens, s. liquefaciens, s. kiliensis, s. grimesii, s. proteamaculans, s. plymutica, s. rubridaea which were also hemolytic. the restriction pattern of the hemolysin locus differed in each strain. s. ficaria and s. marinorubra expressed a different hemolysin ... | 1990 | 2222120 |
| in vitro activity and beta-lactamase stability of the new oral cephalosporin bay v 3522. | the activity of the new oral cephalosporin bay v 3522 was compared to that of six other beta-lactam agents. bay v 3522 inhibited methicillin-susceptible staphylococcus aureus and staphylococcus epidermidis at less than or equal to 2 micrograms/ml, compared to mics of greater than or equal to 8 micrograms/ml for the other cephalosporins tested. it was more active against streptococcus pyogenes (mic less than or equal to 0.06 microgram/ml) than cefuroxime, cefixime, cephalexin and cefaclor. groups ... | 1990 | 2226499 |
| treatment of respiratory tract infections in children: a study of a combination of amoxycillin and clavulanic acid. | in an open study, 70 in-patients and 23 out-patients aged between 1 and 14 years with sinusitis (n = 1), perforated otitis media (n = 4), pharyngotonsillitis (n = 25), tracheobronchitis (n = 30) or broncho-pneumonia (n = 33) were treated daily with a combination of 40 mg/kg amoxycillin and 10 mg/kg clavulanic acid in three equal doses for between 6 and 15 days. purulent specimens were cultured when obtainable and pathogenic organisms identified were staphylococcus aureus, beta-haemolytic strepto ... | 1990 | 2227080 |
| rapid membrane permeabilization and inhibition of vital functions of gram-negative bacteria by bactenecins. | bactenecins are a class of arginine-rich antibacterial peptides of bovine neutrophil granules. two bactenecins with approximate molecular weights of 5,000 and 7,000 designated bac5 and bac7, respectively, exert in vitro a potent bactericidal activity toward several gram-negative bacteria (r. gennaro, b. skerlavaj, and d. romeo, infect. immun. 57:3142-3146, 1989). we have now found that this activity shows an inverse relationship to the ionic strength of the medium and is inhibited by divalent ca ... | 1990 | 2228243 |
| the effect of sodium salicylate on antibiotic susceptibility and synergy in klebsiella pneumoniae. | sodium salicylate was combined with the antibiotics amikacin, aztreonam, cefazolin, cefonicid, cefoperazone, ceftizoxime, norfloxacin, doxycycline, clindamycin, imipenem, mezlocillin and trimethoprim-sulphamethoxazole. the activity of the combinations was tested against encapsulated strains of klebsiella pneumoniae, which differed markedly in their antibiotic susceptibility. the addition of salicylate (from 2 to 350 mg/l) to cultures increased the mic of most antimicrobial agents from two- to fo ... | 1990 | 2228825 |
| the contribution of escherichia coli to microbial colonization resistance. | the contribution of escherichia coli to the microbial colonization resistance (cr) of the bowel was investigated in six healthy volunteers. esch. coli was eliminated from faeces by the administration of a low dose (20 mg daily) of pefloxacin. this did not cause an increase in the faecal concentration of aerobic gram-positive cocci or yeasts, nor did it facilitate colonization of the bowel by a pefloxacin-resistant challenge strain of klebsiella pneumoniae. therefore, esch. coli does not appear t ... | 1990 | 2228829 |
| a three year survey of clinical isolates in the united kingdom and their antimicrobial susceptibility. | a united kingdom national survey of clinical isolates and their antimicrobial susceptibility was performed, with 61 participating hospital laboratories, between 1986 and 1989. each centre used microbe base, a commercial suite of micro-computer programs which can record and analyse antimicrobial susceptibility data informative on 366,853 bacterial isolates and their antimicrobial susceptibility was received from hospital and domiciliary specimens; candida spp. accounted for a further 9121 isolate ... | 1990 | 2228831 |
| assessment of conventional and commercial methods for identification of clinical isolates of cysteine-requiring strains of escherichia coli and klebsiella species. | cysteine-requiring strains of the family enterobacteriaceae that are auxotrophic for this amino acid because of defects in the sulfur assimilatory pathway account for about 1.5% of urinary tract isolates of escherichia coli and klebsiella species. forty escherichia and eight klebsiella cysteine-requiring strains were used to test the ease with which various test systems identified clinical isolates of cysteine auxotrophs. in a preliminary experiment, the growth yield of 10 cysteine-requiring e. ... | 1990 | 2229377 |
| rapid identification of klebsiella. | | 1990 | 2229439 |
| bacterial counts associated with recycled newspaper bedding. | bacterial counts associated with recycled newspaper, wood shavings, and pelleted corn cobs used as bedding for lactating dairy cows were compared. chopped newspaper and pelleted corn cobs had similar gram-negative bacterial, coliform, and streptococcal bedding counts. staphylococcal counts in pelleted corn cobs were greater than in chopped newspaper. conversely, gram-negative bacterial, coliform, and staphylococcal counts in chopped newspaper were greater than in wood shavings. coliform and stre ... | 1990 | 2229587 |
| crepitant myonecrosis caused by klebsiella pneumoniae in an immunocompromised diabetic patient. | | 1990 | 2230280 |
| resistance to apramycin in two enterobacterial clinical isolates: detection of a 3-n-acetyltransferase iv. | considering the possible role of farm animals in the contamination of human consumers by plasmid-mediated apramycin-resistant enterobacteria strains, this type of resistance should be tested more systematically in human isolates. very recently we isolated in zaragoza one apramycin-resistant escheria coli strain obtained from the blood of a hospitalized patient; this clinical isolate produced a plasmid-mediated 3-n-aminoglycoside acetyltransferase iv. we describe also the isolation in madrid of o ... | 1990 | 2230907 |
| isolation of bacterial strains, which hydrolyze glycyrrhizin and produce glycyrrhezic acid, from soil. | we isolated eight bacterial strains which could hydrolyze glycyrrhizin to glycyrrhezic acid. the bacterial strains were identified as three strains of pseudomonas saccharophila, two of plesiomonas sp., one of pseudomonas stutzeri, one of klebsiella pneumoniae subsp. ozaenae and one of kluyvera ascorbata. their capacity for the conversion of glycyrrhizin to glycyrrhezic acid was assayed by high performance liquid chromatography. p. saccharophila 11 was the most effective among the eight strains. ... | 1990 | 2231267 |
| correlation of urinary tract infection with urinary screening at the first antepartum visit. | urinary tract infection contributes to the morbidity of pregnancy since 4-10 percent of antepartum patients have asymptomatic bacteriuria and 20-40 percent of these will result in pyelonephritis. a cost-effective outpatient assessment for asymptomatic bacteriuria in undelivered obstetric patients is described. in this study, 56 patients between 6-24 weeks gestation were assessed over a seven-month period by photometric urinary screening. this assessment was more accurate in identifying those pat ... | 1990 | 2231702 |
| [a clinical study on ceftazidime in the treatment of intractable respiratory infections]. | ceftazidime (caz) was administered to 51 patients (37 males, 14 females) with respiratory infections including severe cases, accompanied by various underlying respiratory diseases. the clinical efficacy and side effects of caz were investigated. the mean age and body weight of these 51 cases were 62.6 years and 48.0 kg, respectively. caz was administered by intravenous drip infusion (daily dose of 2-4 g) for a mean of 14.7 days to a mean total dose of 56.7 g. clinical efficacy rates were 64% (18 ... | 1990 | 2232147 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from urinary tract infections (1987). ii. background of patients]. | in the retrospective survey of the sensitivity of clinical isolates reported in this journal, patients' backgrounds were also investigated. some findings are summarized below. 1. age distribution by sex: patients with ages 50 years and older accounted for a majority, irrespective of their sexes amounting 67.6% for both sexes combined. 2. annual distribution of sexes by age group: females accounted for a majority in younger age brackets, but the proportion of males increased with age, accounting ... | 1990 | 2232149 |
| [synergy between sulbactam and ampicillin or cefoperazone in antimicrobial activity against beta-lactamase producing microorganisms. results with the use of microdilution broth method]. | antimicrobial activities of sulbactam (sbt) with ampicillin (abpc) or with cefoperazone (cpz), in other words, the effects of sbt, an beta-lactamase inhibitor, against beta-lactamase producing strains of clinical isolates, were studied using microdilution broth method. 1. beta-lactamase producing strains such as staphylococcus aureus, branhamella catarrhalis, haemophilus influenzae, escherichia coli and klebsiella pneumoniae decompose benzylpenicillin (pcg) which is one of substrates of the acid ... | 1990 | 2232151 |
| antimicrobial activity of naphthoquinones from fusaria. | twenty-two naphthoquinone compounds isolated or derived synthetically from culture extracts of fusarium solani and f. oxysporum were examined for antimicrobial activity. fifteen exhibited antibiotic activity against staphylococcus aureus, and 12 were active against streptococcus pyogenes, but none were active at the highest rate of 128 micrograms/ml against escherichia coli, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, serratia marcescens, or pseudomonas aeruginosa. of 8 plant path ... | 1990 | 2233983 |
| nosocomial bacteremias in measles. | the purpose of this study was to determine whether nosocomial bacteremias occurred more frequently in patients admitted with severe measles compared with general pediatric admissions. in a retrospective survey of 977 blood culture reports during a 4-year period, 1985 to 1988, the incidence of nosocomial bacteremias in patients with measles was found to be on the average of 3.37/100 admissions/year, approximately 6 times that of general pediatric patients (0.57). gram-negative organisms (predomin ... | 1990 | 2235144 |
| the effect of biostim (ru-41740) on the expression of cytokine mrnas in murine peritoneal macrophages in vitro. | the immunomodulatory agent biostim (ru-41740) was investigated for its ability to induce the expression of cytokine mrnas in murine peritoneal macrophages in vitro. northern blot analysis showed that in quiescent macrophage populations, both il-1 alpha and il-1 beta mrna levels were dramatically increased in response to 1 microgram/ml biostim. dot-blot analysis showed that in quiescent macrophage populations the expression of mrnas for il-1 alpha, il-1 beta, il-6 and tnf-alpha could be elevated ... | 1990 | 2237939 |
| [a case of fournier's gangrene: was it triggered by prostatic massage?]. | we have experienced a case of fournier's gangrene which progressed rapidly after prostatic massage. the patient was a 70-year-old man who had poorly controlled diabetes mellitus, hemorrhoid, urethral stricture and benign prostatic hyperplasia. he visited an urologist complaining of pollakisuria and miction pain. under the diagnosis of prostatitis, prostatic massage was performed. from that night, he developed a high grade fever. simultaneously, redness, swelling and pain of the scrotum progresse ... | 1990 | 2239620 |
| colobronchial fistula: a rare complication of crohn's colitis. | a 29-yr-old white woman presented with chronic pneumonia in the left lower lobe and with left pleural effusion. she was known to have inflammatory bowel disease, but she was asymptomatic under maintenance treatment with 5-asa. she received numerous antibiotic regimens according to susceptibility testing of microorganisms cultured from sputum and bronchial lavage and on an empiric basis was also given antituberculosis treatment, but there was no clinical improvement or change in the chest radiogr ... | 1990 | 2240849 |
| substitution of gly-96 to ala in the 5-enolpyruvylshikimate-3-phosphate synthase of klebsiella pneumoniae results in a greatly reduced affinity for the herbicide glyphosate. | the aroa gene of klebsiella pneumoniae encoding the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate (epsp) synthase, which is the target of the herbicide glyphosate, was cloned and sequenced from both the wild-type and the glyphosate-resistant mutant k. pneumoniae k1, which possesses a glyphosate-insensitive epsp synthase. both genes were expressed in escherichia coli and were capable of complementing an auxotrophic aroa mutation. the transformed cells showed increased tolerance to g ... | 1990 | 2241161 |
| [studies on respiratory infections in primary care clinic (iii). distribution of bacteria isolated from patients with respiratory infections visiting 21 private clinics in the tohoku district of japan]. | the bacteriology of the isolates from the throat swab and the sputum respectively of 2,539 patients with respiratory infections visiting 21 private clinics in tohoku district of japan during the period from january to april in 1989 was documented. of the 2,539 patients, 1,694 had an acute upper respiratory infection, 609 had acute bronchitis, 46 had acute pneumonia, 84 had acute exacerbation of chronic respiratory infections and 106 had respiratory infections without diagnosis registered. 1887 ( ... | 1990 | 2243193 |
| [studies on respiratory infections in primary care clinic (iv). antibiotic sensitivity of bacteria isolated from patients with respiratory infections visiting 21 private clinics in the tohoku district of japan]. | we determined the mics of ampicillin, methicillin, cefaclor, cefixime, cefteram, ofloxacin and ciprofloxacin against a total of 1,448 strains from 11 species: 464 strains of staphylococcus aureus, 306 strains of streptococcus pneumoniae, 114 strains of streptococcus pyogenes, 37 strains of branhamella catarrhalis, 329 strains of haemophilus influenzae, 32 strains of escherichia coli, 66 strains of klebsiella pneumoniae, 26 strains of enterobacter cloacae, 20 strains of serratia marcescens, 12 st ... | 1990 | 2243194 |
| primary purulent pericarditis following renal transplantation. | | 1990 | 2246208 |