| long-term clearance of inhaled uo2 particles from the pulmonary region of the rat. | this paper reports on an enriched uranium dioxide (uo2) mass clearance study undertaken with fischer-344 rats. the uo2 had a uranium (u) isotopic composition of 0.79% 234u, 92.8% 235u, 0.34% 236u and 6.06% 238u, by mass, with an alpha-particle activity of 1.91 bq micrograms-1. forty-six rats were exposed to an enriched uo2 aerosol that had an activity median particle aerodynamic diameter ranging from 2.7 to 3.2 microns. the rats were killed from 1 to 720 d post-inhalation (pi). the mass of enric ... | 1990 | 2323929 |
| novel antibiotics, furaquinocins a and b. taxonomy, fermentation, isolation and physico-chemical and biological characteristics. | two novel antibiotics, furaquinocins a and b were isolated from the culture broth of streptomyces sp. ko-3988. these antibiotics possess cytocidal activities against hela s3 cells in vitro at concentrations of 3.1 micrograms/ml for a and 1.6 micrograms/ml for b. neither substance possessed antimicrobial activities against gram-positive and gram-negative bacteria, fungi or yeast at a concentration of 1,000 micrograms/ml. | 1990 | 2324009 |
| vibrio cholerae bacteremia associated with gastrectomy. | bacteremia due to vibrio cholerae is rare. each of 15 cases previously reported in the english language literature occurred in the setting of immune deficiency. we describe an instance of non-serogroup o1 v. cholerae septicemia in an otherwise healthy patient. susceptibility to such infection may have been enhanced by a prior gastrectomy for duodenal ulcer. | 1990 | 2324281 |
| lipopolysaccharides of non-cholera vibrios possessing common antigen factor to 01 vibrio cholerae. | | 1990 | 2327294 |
| [the toxin-producing capacity of populations of vibrio cholerae of different origins]. | as the result of the study of toxin formation in 165 v. eltor strains having different virulence, most of the virulent cultures have shown stable toxin production, though 5-10% of their colonies have proved to be nontoxigenic. in faintly virulent strains toxin production was found to be unstable, which is indicative of the heterogeneity of their population composition as regards their capacity for toxin formation. the population of avirulent strains consists mainly of nontoxigenic clones (95.7%) ... | 1990 | 2330784 |
| effects of dnase production, plasmid size, and restriction barriers on transformation of vibrio cholerae by electroporation and osmotic shock. | attempts to transform wild type strains of v. cholerae with plasmid dna by traditional osmotic shock methods were not successful. a mutant of v. cholerae that was deficient in extracellular dnase was transformed with plasmid dna by osmotic shock, demonstrating directly that extracellular dnase is a major barrier to transformation of v. cholerae. transformation of wild type and dnase-negative strains of v. cholerae was accomplished by electroporation. efficiency of transformation by electroporati ... | 1990 | 2332153 |
| analysis of the 2-keto-3-deoxyoctonate (kdo) region of lipopolysaccharides isolated from non-01 vibrio cholerae 05r. | phosphorylated 2-keto-3-deoxyoctonate (kdo) has been detected in the strong-acid hydrolysates of lipopolysaccharides (lps) of family vibrionaceae including vibrio cholerae. structural analysis of lps isolated from a rough mutant of non-01 v. cholerae 05 by dephosphorylation, periodate oxidation and methylation analysis revealed that the inner core region of the lps molecule contains only one mole of kdo in contrast to enteric gram-negative bacterial lps, and that the phosphate group on the kdo m ... | 1990 | 2332154 |
| effect of iron on production of a possible virulence factor by plesiomonas shigelloides. | plesiomonas shigelloides, when grown in an iron-poor medium (syncase), produces a substances that causes elongation of chinese hamster ovary (cho) cells similar to that produced by cholera toxin. when syncase is supplemented with iron, the ability of p. shigelloides (but not of vibrio cholerae) to produce this elongation of cho cells is lost. iron depletion of the growth medium appears to be essential for the cho cell elongation produced by p. shigelloides but is not essential for the production ... | 1990 | 2332475 |
| biotype-specific probe for vibrio cholerae serogroup o1. | the o1 serogroup of vibrio cholerae can be divided into two biotypes, el tor and classical. current tests to distinguish between these biotypes are often difficult to interpret. on the basis of the difference in sequence of the hlya gene in these biotypes, we have developed a simple probe that can easily and reliably differentiate between the two biotypes. | 1990 | 2332478 |
| nucleotide sequence of the gene, ompw, encoding a 22kda immunogenic outer membrane protein of vibrio cholerae. | | 1990 | 2336399 |
| person-to-person transmission of cholera in a psychiatric hospital. | an outbreak of cholera caused by vibrio cholerae o1, biotype el tor, serotype ogawa, phage type 4, was reported in a psychiatric hospital in singapore. a total of 74 inmates (18 symptomatic and 56 asymptomatic) were infected; two of them died. extensive epidemiological investigations showed that the organism was not transmitted by contaminated food or water but through close person-to-person contact. early recognition of the outbreak and prompt implementation of epidemic control measures compris ... | 1990 | 2341728 |
| immunobiological relationships of the enterotoxins produced by cholera toxin gene-positive (ct+) and -negative (ct-) strains of vibrio cholerae o1. | the optimum rabbit ileal loop (ril) reacting doses of the new cholera toxin (nct) produced by cholera toxin gene-negative (ct-) strain x-392 and of the enterotoxin produced by cholera toxin gene-positive (ct+) strain 569b of vibrio cholerae o1 were found to be 32 micrograms and 22 micrograms respectively. production of nct by the ct+ strain, in addition to ct, was confirmed by in-vivo neutralisation tests. anti-569b-enterotoxin neutralised the optimum ril reacting activity of nct completely at 1 ... | 1990 | 2342085 |
| fatalities associated with vibrio parahaemolyticus and vibrio cholerae non-o1 infections in florida (1981 to 1988). | vibrio infections constitute a continuing source of morbidity and mortality in florida. seven fatal infections caused by vibrio parahaemolyticus or v cholerae non-o1 were reported in florida between 1981 and 1988. review of those seven medical records and vibrio case investigation forms showed that although all patients died of sepsis, gastrointestinal signs and symptoms characterized the early illness in four patients, whereas the other three initially had painful swelling and/or lesions of the ... | 1990 | 2343327 |
| [inheritance and expression of cholera toxin genes introduced into vibrio cholerae el tor cells in a hybrid plasmid]. | two replicons, pox38 (in f-factor derivative lacking all is elements) and pct105 (containing cholera toxin operon cloned in pbr322) have been fused to produce recombinant plasmid, pco109, which was then introduced into vibrio cholerae eltor by conjugation. restriction analysis showed pco109 to dissociate in v. cholerae cells at a higher frequency than in escherichia coli strains, its pox38 component being lost, while the pct105 component demonstrated relative stability. v. cholerae eltor rv79 (p ... | 1990 | 2344953 |
| physical demonstration of a high molecular weight bacteriocin plasmid in vibrio cholerae by genetic transformation process. | bacteriocinogeny was transferred at high frequencies from bacteriocinogenic (bac+) v. cholerae strains to non-bacteriocinogenic (bac-) recipients in the in situ genetic transformation system on agar surface. dna extracted from samples of growth of bacteria transformed to bac+ were obtained at 2 h intervals following contact with the sterile agar surface where the donor had grown previously. this showed acquisition of a high molecular weight plasmid which could be physically demonstrated best in ... | 1990 | 2345019 |
| cholera epidemic in solapur during july-august, 1988. | of the 77 vibrio cholerae isolated during july to september, 1988, 76 were el tor vibrios serotype ogawa, 68 belonging to t2 phage (eight strains untypable). peak incidence was noted in the month of august, 1988. haemolytic and non-haemolytic colony variants of el tor v. cholerae were encountered. all strains showed resistance to one or more antibiotics. no fatality was reported during the epidemic. the epidemiological data collected over the past three years (1986-88) show that cholera is preva ... | 1990 | 2345026 |
| transferable trimethoprim resistance of vibrio cholerae o1 encountered in southern india. | | 1990 | 2345916 |
| inhibitory effect of calcium-binding protein regucalcin on protein kinase c activity in rat liver cytosol. | regucalcin, a calcium-binding protein isolated from rat liver cytosol, inhibited ca2(+)- and phospholipid-dependent protein kinase (protein kinase c) activity in hepatic cytosol. with the increasing concentrations of ca2+ or phosphatidylserine in the medium, regucalcin caused a remarkable inhibition of protein kinase c activity. moreover, regucalcin significantly inhibited dioctanoylglycerol-activated protein kinase c. regucalcin itself did not have protein kinase activity in either the presence ... | 1990 | 2346670 |
| phase-transfer-catalyzed synthesis of aryl alpha-ketosides of n-acetylneuraminic acid. a 2-methylfluoran-6-yl glycoside of n-acetylneuraminic acid, 2-methyl-6-(5-acetamido-3,5-dideoxy-alpha-d-glycero-d-galacto- nonulopyranosylonic acid)xanthene-9-spiro-1'-isobenzofuran-3'-one, a new substrate for neuraminidase assay. | glycosidation of n-acetylneuraminic acid by phase-transfer catalysis in chloroform-aqueous alkali gave several known and some new aryl alpha-ketosides in a short reaction time and in good yields. the 4-methylumbelliferyl alpha-ketoside, the standard substrate for neuraminidase, was prepared in a yield of up to 70%. new neu5ac ketosides were prepared with fluorescein and the fluorescein analog, 2-methyl-6-hydroxyfluoran (2-methyl-6-hydroxyxanthene-9-spiro-1'-isobenzofuran-3'-one) as aglycons, the ... | 1990 | 2346942 |
| bismuth subsalicylate in the prevention of colonization of infant mice with campylobacter jejuni. | infant mice were used for the evaluation of the efficacy of bismuth subsalicylate (bss) in the prevention of the growth of campylobacter jejuni in the intestine. the mic90 of ten c. jejuni strains was 900 micrograms/ml. of three dosage regimens tested, continuous treatment before and after the bacterial challenge, mimicking the way bss is used in the prevention of traveller's diarrhoea, was the most effective. growth inhibition was dose dependent; the high dose of 2000 micrograms per day was mor ... | 1990 | 2347383 |
| the effect of endotoxin on membrane fatty acid composition in bcg-sensitized mice. | the effects of endotoxin on mouse liver phospholipid fatty acid composition have been investigated. administration of endotoxin from salmonella abortus equi led to a decrease in the polyunsaturated fatty acid content of livers from mice sensitized with bacille calmette guérin (bcg). the content of arachidonic acid fell significantly in both the phosphatidylcholine and phosphatidylinositol fractions whereas in the phosphatidylethanolamine fraction the linoleic acid content was significantly reduc ... | 1990 | 2347397 |
| lipid a mutants of vibrio cholerae: isolation and partial characterization. | vibrio cholerae mutants resistant to common antibiotics and neutral and anionic detergents were isolated. analysis of isolated outer membranes revealed a significant deficiency in the acylation of lipid a in the resistant strains. the content of amide-linked and ester-bound fatty acids in the lipid a of the mutant strains compared to that of the wild type was about 50-56% and 29-37% respectively. this defect was specific for lipid a as there was no change in the acylation of phospholipids. the r ... | 1990 | 2350336 |
| further studies on the gangliosidic nature of the cholinergic-specific antigen, chol-1. | the antigen designated as chol-1 beta, detected by an antiserum specific for cholinergic neurons, has been purified to homogeneity from ganglioside mixtures extracted from torpedo electric organ and pig brain. the final products from the two sources behaved identically in a wide range of tests and gave coincident immunopositive and ehrlich-positive spots after thin layer chromatography in seven different solvent systems; they were thus considered to be identical and to constitute a single, pure ... | 1990 | 2353821 |
| [bacteremia caused by vibrio cholerae no. 01]. | | 1990 | 2355781 |
| morphology of endotoxin-like particles released by vibrio cholerae non-o1. | the morphology of "free endotoxin" in cell suspensions of vibrio cholerae non-o1 and in fractions purified from culture filtrate (fraction 1) was examined at the beginning of the exponential phase of growth in complex medium. the observed structures were compared with those of the lipopolysaccharide obtained by phenol extraction. blebs, vesicles, and ribbons with a trilaminar, membrane-like structure were observed by electron microscopy of ultrathin sections and negatively stained samples. endot ... | 1990 | 2363795 |
| intestinal cryptosporidiosis acquired from a cat. | a case of intestinal cryptosporidiosis in an eight-year-old boy is presented. the patient became ill during a visit to a farm where diarrhoea in newborn calves is a recurrent problem. furthermore, on that farm kittens periodically suffer from diarrhoea and failure to thrive. oocysts of cryptosporidium sp. were identified in the stool of the patient, and in the stool of the cat he had contact with. at that time the calves were not infected. the patient's gastrointestinal symptomatology consisted ... | 1990 | 2365471 |
| intestinal cryptosporidiosis acquired from a cat. | a case of intestinal cryptosporidiosis in an eight-year-old boy is presented. the patient became ill during a visit to a farm where diarrhoea in newborn calves is a recurrent problem. furthermore, on that farm kittens periodically suffer from diarrhoea and failure to thrive. oocysts of cryptosporidium sp. were identified in the stool of the patient, and in the stool of the cat he had contact with. at that time the calves were not infected. the patient's gastrointestinal symptomatology consisted ... | 1990 | 2365471 |
| kinetics of "p"-site-mediated inhibition of adenylyl cyclase and the requirements for substrate. | the kinetics of "p"-site-mediated inhibition of adenylyl cyclase was studied with the detergent-solubilized enzyme from rat brain. mn2(+)-activated adenylyl cyclase exhibited typical noncompetitive inhibition by 2'-d3'-amp or 2',5'-dideoxyadenosine (2',5'-ddado). however, enzyme that was preactivated with guanosine 5'-o-(3-thiotriphosphate) (gtp gamma s) or proteolytically with ninhibin (+ gtp gamma s) exhibited apparently uncompetitive inhibition with either 2'-d3'-amp or 2',5'-ddado and with e ... | 1990 | 2365688 |
| the stereospecificities of seven dehydrogenases from acholeplasma laidlawii. the simplest historical model that explains dehydrogenase stereospecificity. | stereospecificities are reported for seven dehydrogenases from acholeplasma laidlawii, an organism from an evolutionarily distinct branch of life which has not previously been studied from a stereochemical point of view. three of the activities examined (alcohol dehydrogenase, lactate dehydrogenase, and alanine dehydrogenase) catalyze the transfer of the pro-r (a) hydrogen from nadh. four other activities (3-hydroxy-3-methylglutaryl-coa reductase, glyceraldehyde-3-phosphate dehydrogenase, glucos ... | 1990 | 2365693 |
| the cytolysin gene of vibrio vulnificus: sequence and relationship to the vibrio cholerae e1 tor hemolysin gene. | a cytolysin of ca. 56 kilodaltons has been suggested as a possible virulence factor in vibrio vulnificus infections. we sequenced the dna encoding cytolytic activity and found that the sequence contained two open reading frames, vvha and vvhb. vvha encoded the structural gene for the cytolysin and contained the n-terminal amino acid sequence previously reported for the protein. regions of the vvha gene showed homology to the structural gene for the vibrio cholerae e1 tor hemolysin. | 1990 | 2370116 |
| glycosphingolipid expression on murine l1-fibrosarcoma cells: analysis of clonal in vivo and in vitro selected sublines with different lung colonisation potential. | the patterns of acidic and neutral glycosphingolipids (gsls) were examined in a syngeneic tumour system in balb/c mice consisting of closely related cell lines with different colonisation potentials directed to the murine lungs (in vivo selected highly metastatic sublines of l1-fibrosarcoma cells and their wga-resistant mutants with low metastatic potential). gsls were analysed by high-performance thin-layer chromatography and structurally identified by fast atom bombardment mass spectrometry co ... | 1990 | 2372482 |
| [the equivalent resistance of intracerebral gold electrodes in human beings]. | | 1990 | 2373308 |
| temporal regulation of hyaluronan and proteoglycan metabolism by human bone cells in vitro. | osteoblasts elaborate a dynamic extracellular matrix that is constructed and mineralized as bone is formed. this matrix is primarily composed of collagen, along with noncollagenous proteins which include glycoproteins and proteoglycans. after various times in culture, human bone cells were labeled with [35s]sulfate, [3h] leucine/proline, or [3h]glucosamine and the metabolism of hyaluronan and four distinct species of proteoglycans (pgs) was assayed in the medium, cell layer, and intracellular po ... | 1990 | 2373688 |
| the downfield resonances in the 1h nmr spectra of azotobacter vinelandii and pseudomonas putida seven-iron ferredoxins. | pseudomonas putida and azotobacter vinelandii ferredoxins each contain one [4fe-4s] cluster and one [3fe-4s] cluster. their polypeptide chains are nearly identical, differing by only 15 residues out of a total of 106. t1 measurements and temperature dependence studies of the 1h nmr spectrum of each ferredoxin demonstrate that all six resolved downfield resonances are near an iron-sulfur center. the five most downfield resonances are shown to arise from protons on cysteinyl beta-carbons by incorp ... | 1990 | 2373698 |
| bacteria from solid tumours. | bacteria were grown from 63 (69%) of 91 specimens from necrotic tumours in 63 patients. of the tumours, 14 were abdominal, 5 pelvic, 23 of the head and neck, 4 of the lungs, 4 mediastinal, 2 lymphatic, 3 of the breast, and 8 were miscellaneous. aerobic or facultatively anaerobic bacteria only were present in 12 (19%) specimens, anaerobes only in 10 (16%), and mixed aerobic and anaerobic bacteria in 41 (65%). a total of 83 anaerobic and 47 aerobic and facultatively anaerobic bacteria were isolate ... | 1990 | 2374159 |
| cloning and nucleotide sequence of a cdna encoding the antifungal-protein of aspergillus giganteus and preliminary characterization of the native gene. | | 1990 | 2374718 |
| [the morphofunctional characteristics of lymphoma nk/ly cells treated with concanavalin a and vibrio cholerae neuraminidase]. | mouse nk/ly lymphoma cells were modified by con a or vibrio cholerae neuraminidase (vcn). severe damage of the modified cells was not revealed by either supravital staining or light microscopy. ultrastructural signs of metabolic lowering in the con-a-treated cells were determined. some increase in electrophoretic mobility of con-a-modified cells was found, along with its decrease in vcn-modified cells. the decrease in the pool of proliferating cells was determined in both the types of modificati ... | 1990 | 2375060 |
| [prevalence of type 1 human immunodeficiency virus (hiv) infection in african patients residing in catalonia]. | 278 black patients from west africa, living in maresme, a coastal area north of barcelona were studied in order to determine the prevalence of hiv-1 infection. during the first period (1984-86), 102 patients were studied, 3 of them were positive for hiv-2, but none was positive for hiv-1. in the second period (1987-89), 176 patients were studied and 15 were positive for hiv-1. sexual intercourse with prostitutes of low social level was the only risk factor in all of them. nine of these 15 patien ... | 1990 | 2377013 |
| use of a synthetic oligonucleotide probe to detect strains of non-serovar o1 vibrio cholerae carrying the gene for heat-stable enterotoxin (nag-st). | a synthetic oligonucleotide probe was developed to identify the gene for the heat-stable enterotoxin (nag-st) of non-serovar o1 vibrio cholerae. of 103 non-o1 v. cholerae isolates from thailand, 31 isolates from mexico, and 47 isolates from patients in the united states, only 7 (all from thailand) hybridized with the probe. probe-positive strains produced significantly higher fluid accumulations in infant mice than probe-negative strains. | 1990 | 2380369 |
| vibrio bacteremia in siriraj hospital. | from january 1983 to march 1988, 26 isolates of vibrio spp. were recovered from the blood of patients admitted to siriraj hospital. thirteen strains were identified as non 0-1 vibrio cholerae, 3 were vibrio vulnificus and 10 were vibrio spp. the medical records of 20 patients were available for clinical analysis. most of them were adult men with cirrhosis. clinical features included fever, abdominal pain, diarrhea, peritonitis, shock and skin lesions. some patients had a history of seafood consu ... | 1990 | 2380645 |
| [antibacterial and anti-hemolysin activities of tea catechins and their structural relatives]. | among catechins tested, (-)epigallocatechin (egc), (-)epicatechin gallate (ecg), (-) epigallocatechin gallate (egcg) inhibited the growth of staphylococcus aureus, vibrio cholerae o1 classical inaba 569b and el tor inaba v86. s. aureus was more sensitive than v. cholerae o1 to these compounds. egcg showed also a bactericidal activity against v. cholerae o1 569b. pyrogallol showed a stronger antibacterial activity against s. aureus and v. cholerae o1 than tannic and gallic acid. rutin or caffein ... | 1990 | 2381042 |
| attachment of vibrio cholerae serogroup o1 to zooplankton and phytoplankton of bangladesh waters. | vibrio cholerae serogroup o1, the causative agent of cholera, is capable of surviving in aquatic environments for extended periods and is considered an autochthonous species in estuarine and brackish waters. these environments contain numerous elements that may affect its ecology. the studies reported here examined physical interactions between v. cholerae o1 and natural plankton populations of a geographical region in bangladesh where cholera is an endemic disease. results showed that four of f ... | 1990 | 2383016 |
| [groups of the species vibrio cholerae having different epidemic importance]. | subdivision of v. cholerae 01 into toxins on the basis of the whole complex of signs characteristic of this species does not make it possible to judge on their epidemic importance and to use the data on identification of v. cholerae for solving practical problems. classification of v. cholerae by their capacity for producing toxin (choleragen and hemolysin of type 1, subtype beta) removes these difficulties. | 1990 | 2385990 |
| isolation and structure of a novel charged member of the red-pigment-concentrating hormone-adipokinetic hormone family of peptides isolated from the corpora cardiaca of the blowfly phormia terraenovae (diptera). | a hypertrehalosaemic neuropeptide from the corpora cardiaca of the blowfly phormia terraenovae has been isolated by reversed-phase h.p.l.c., and its primary structure was determined by pulsed-liquid phase sequencing employing edman chemistry after enzymically deblocking the n-terminal pyroglutamate residue. the c-terminus was also blocked, as indicated by the lack of digestion when the peptide was incubated with carboxypeptidase a. the octapeptide has the sequence pglu-leu-thr-phe-ser-pro-asp-tr ... | 1990 | 2386478 |
| role of the p plasmid in attenuation of vibrio cholerae o1. | the conjugative plasmid p of vibrio cholerae has been shown to have a suppressive effect on the virulence of hypertoxigenic strains like 569b. in this study, we have sought to analyze this phenomenon. utilizing the infant mouse cholera model, we have demonstrated that the presence of p increases the 50% lethal dose of v. cholerae classical inaba 569b by more than 300-fold. no effect of p on cholera toxin (ct) production, whether measured by gm1 enzyme-linked immunosorbent assay, by ct activity i ... | 1990 | 2387637 |
| identification of a virb10 protein aggregate in the inner membrane of agrobacterium tumefaciens. | products of the virb operon are proposed components of a membrane-associated t-dna transport apparatus in agrobacterium tumefaciens. here we identified the virb10 gene product and raised specific antiserum to the protein. while the virb10 reading frame contains two potential atg translation start sites located 32 codons apart, we found that only the downstream atg was required for efficient virb10 synthesis. cellular localization studies and analysis of translational fusions with the escherichia ... | 1990 | 2394684 |
| the physics of spinal manipulation. part iii. some characteristics of adjusting that facilitate joint distraction. | the role of quickness, adjustive amplitude, preadjustive tension, and countertension in facilitating joint distraction to cavitation is investigated. these characteristics are further shown to contribute to joint isolation, protection of joints from injury, and enhanced adjustive efficiency. block and spring "slinky dog" models aid in illustrating the physics principles involved. the complete role of mass and acceleration in the adjustive process is discussed. | 1990 | 2394947 |
| sex differences in hormonal responses of vasopressin pathways in the rat brain. | vasopressin (avp) immunoreactivity in cells and projections of the bed nucleus of the stria terminalis (bst) and medial amygdaloid nucleus (ma) depends on gonadal steroids. in addition, the avp projections from the bst show denser fiber staining in males than in females. to study whether these differences depend on different hormone levels in adulthood, male and female rats were gonadectomized and similarly treated with testosterone for 4 weeks prior to sacrifice. immunocytochemistry showed that ... | 1990 | 2394985 |
| are separable aromatase systems involved in hormonal regulation of the male brain? | in vitro study of testosterone (t) metabolism shows that formation of estradiol-17 beta (e2) is regionally specific within the preoptic area (poa) of the male ring dove. the poa is known to be involved in the formation of e2 required for specific components of male sexual behavior. two sub-areas of high aromatase activity, anterior (apoa) and posterior preoptic (ppoa) areas, have been identified. aromatase activity is higher in apoa than in ppoa. the aromatase activity within the apoa is also mo ... | 1990 | 2394989 |
| toxicity of intravesical recombinant human tumor necrosis factor in cynomolgus monkeys. | six groups of two cynomolgus monkeys were treated with escalating intravesicular doses of recombinant human tumor necrosis factor (rhutnf) for a 6 week interval. the doses of rhutnf ranged from 10 ng to 1 mg and were instilled weekly. two monkeys had instillation of saline only and served as controls. the monkeys were weighed and temperatures determined before, immediately after, and 2 days following each treatment. cystoscopic examination was performed 2 days after each treatment and blood samp ... | 1990 | 2395009 |
| metabolism of indium-111-labeled murine monoclonal antibody in tumor and normal tissue of the athymic mouse. | we have studied the fate of radiometal metabolism in vivo by analyzing the molecular form of radiolabeled anti-carcinoembryonic antigen (cea) in tissues. athymic mice bearing colonic tumor xenografts were injected i.v. with 111in-isothiocyanate-benzyl-dtpa-(scn-bz-dtpa) igg or 111in-(scn-bz-dtpa) f(ab')2, and then killed daily for up to four days. liver, kidney, and tumor were extracted and the supernatants, plasma and urine samples were analyzed by hplc, itlc, hpiec and sds-page. by hplc, the a ... | 1990 | 2395019 |
| [testosterone metabolism in discrete areas of the brain in rat fetuses]. | experiments in vitro with further thin-layer chromatography of steroids were staged to investigate changing of 3h-testosterone into 3h-estradiol-17 beta and 3h-5 alpha-reduced metabolites in a fraction of 1000 g of homogenates of the medial preoptic area (mpa), the paraventricular nuclei (pvn), the dorsal vagal complex (dvc) of the lower cerebral trunk, the rostral and caudal parts of the amygdaloid complex (acr and acc) of the brain of 21-day male and female rat fetuses. the activity of aromata ... | 1990 | 2395838 |
| acute, chronic, and interactive effects of type i and ii corticosteroid receptor stimulation on feeding and weight gain. | type i (aldosterone) and/or type ii (dexamethasone or ru28362) corticosterone receptor agonists were continuously infused in adrenalectomized sprague-dawley rats for 28 days at doses of 3.4, 17.2, or 86.2 nmol/day. additional groups received combined agonist infusions, blank infusions, or sham operations. the type i agonist stimulated body weight gain, and the type ii agonists were both suppressive, differing mainly in degree. although there were a few early effects of these hormones (usually a ... | 1990 | 2395928 |
| prepuberal mpoa lesions and housing condition: effects on rats' male sex behaviors. | after prepuberal male rats underwent either medial preoptic area (mpoa) lesions or sham surgeries, they were housed in all-male groups (soc-x and soc-s treatments) or isolation (iso-x and iso-s treatments). in the first mating test, soc-x rats mounted and intromitted more than iso-x, but not soc-s, rats. in this and across mating tests, soc-x did not differ from soc-s rats in the proportion ejaculating; however, fewer iso-x than iso-s rats ejaculated. in the first ejaculatory test, mpoa-lesioned ... | 1990 | 2395934 |
| [genomic "dactyloscopy" in the expertise of disputed paternity and the determination of biological relationship]. | genome "dactyloscopy" (dna fingerprinting) is a principally new way of personal identification based on analysis of human genetic material (dna); the difference in dna structure of different subjects is the scientific basis of this method. this ensures opportunity to estimate biological relationship of persons positively. the authors were the first to demonstrate using certain expert material the adequacy and potentials of dna fingerprinting by m 13 probe for medicolegal expert practice in most ... | 1990 | 2396289 |
| non-01 vibrio cholerae septicemia: a case report. | | 1990 | 2402675 |
| aetiological studies of diarrhoeal diseases in infants and young children in iran. | the aetiology of diarrhoeal diseases was investigated among 715 patients admitted to four children's hospitals in tehran, during february 1986 to march 1987, and also among 443 patients attending the central out-patient clinic in sanandaj, state of kordestan, during october 1986. enteropathogenic escherichia coli (epec) were the most common pathogens found in both areas. almost 26.7% of the patients in tehran and 20.1% of the patients in sanandaj were infected with epec. enterotoxigenic e. coli ... | 1990 | 2406458 |
| in vitro antimicrobial activity of bismuth subsalicylate and other bismuth salts. | this report demonstrates that bismuth subsalicylate (bss) effectively inhibits growth of a number of bacterial strains known to cause diarrhea, including escherichia coli, salmonella, shigella, and campylobacter. other bismuth salts and sodium salicylate, a hydrolysis product of bss in the gut, also were examined and were shown to have various degrees of activity. growth of the organisms was monitored in vitro by inoculating culture fluid that contained one of the compounds to be tested and dete ... | 1990 | 2406851 |
| fluorescent staining of sialidases in polyacrylamide gel electrophoresis and ultrathin-layer isoelectric focusing. | polyacrylamide gels were stained with the sialidase substrate 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid showing the activity of vibrio cholerae and clostridium sordellii sialidases in the gels after electrophoresis. with this fluorogenic method minimum sialidase activities of 5 microu could be determined. the sensitivity of this staining is about 10,000-fold higher compared to protein-staining with coomassie brilliant blue. for the visualization of other proteins than sialidases ... | 1985 | 2409836 |
| use of non-carbonated soft drinks to provide safe drinking water. | non-carbonated, low-calorie soft drink concentrates (cordials), when diluted according to manufacturers' instructions, had significant antibacterial effects in vitro. bacteria affected include vibrio cholerae, aeromonas hydrophila, shigella sonnei, salmonella typhimurium and escherichia coli. with vibrios, bacterial counts were reduced from 10(6)/ml to undetectable numbers in less than 10 min. escherichia coli in an initial concentration of 10(6)/ml became undetectable after incubation for 1 h w ... | 1985 | 2409896 |
| catalase enhances damage to dna by bleomycin-iron(ii): the role of hydroxyl radicals. | bleomycin degrades dna under aerobic conditions when a ferrous salt is added. this reaction is enhanced by catalase and certain hydroxyl radical scavengers but inhibited by the addition of hydrogen peroxide. a ferricbleomycin complex is, however, stimulated by addition of hydrogen peroxide. these findings suggest that catalase removes hydrogen peroxide and in so doing prevents loss of ferrous ions and formation of hydroxyl radicals (oh.) by a fenton-type reaction. it further suggests that oh. ra ... | 1985 | 2409975 |
| rapid detection of vibrio cholerae o:1 by motility inhibition and immunofluorescence with monoclonal antibodies. | monoclonal antibodies against the group and type specific antigens of vibrio cholerae o:1 lipopolysaccharide were used for the rapid detection of vibrio cholerae strains by motility inhibition and immunofluorescence. motility inhibition of live vibrio cholerae o:1 was obtained with group specific monoclonal antibodies. monoclonal antibodies against the type specific antigens b (ogawa) and c (inaba) inhibited motility of strains of homologous serotypes only. indirect immunofluorescence of heatfix ... | 1985 | 2410265 |
| immunological characterization of vibrio cholerae o:1 lipopolysaccharide, o-side chain, and core with monoclonal antibodies. | lipopolysaccharide (lps) was extracted from vibrio cholerae o:1 strains of the serotypes ogawa, inaba, and hikojima and delipidated by mild-acid hydrolysis. two polysaccharide fragments with the molecular weights of approximately 9,000 and 900, respectively, were isolated by gel permeation chromatography. the lps preparations and the polysaccharide fragments were studied in enzyme-linked immunosorbent assay inhibition, rocket immunoelectrophoresis, and sodium dodecyl sulfate-polyacrylamide gel e ... | 1985 | 2410362 |
| membrane-bound 5'-nucleotidase in marine luminous bacteria: biochemical and immunological properties. | a novel 5'-nucleotidase previously described in halophilic vibrio costicola was detected in marine vibrio and photobacterium strains. the enzyme of marine bacteria was similar in its properties to the 5'-nucleotidase of vibrio costicola; it was outwardly oriented in the cytoplasmic membrane and dephosphorylated nucleoside 5'-tri-, di-, and mono-phosphates to respective nucleosides before uptake. the enzyme in marine strains was immunologically cross-reactive with the antibody raised against the ... | 1985 | 2411368 |
| [basic factors of the pathogenicity of vibrio cholerae]. | | 1985 | 2412368 |
| a study on the pharmacokinetics in mouse of adenine-9-beta-d-arabinofuranoside 5-monophosphate conjugated with lactosaminated albumin. | in plasma of mice injected with adenine-9-beta-d-arabinofuranoside monophosphate (ara-amp) coupled to human lactosaminated serum albumin (l-hsa) some of the ara-amp molecules are enzymatically released, whereas others remain linked to l-hsa. evidence has been obtained that ara-amp is not deaminated when it is conjugated to l-hsa, in contrast to the free drug which is rapidly metabolized to its hypoxanthine derivative. | 1985 | 2412878 |
| a simple method for counting nuclei in the preimplantation mouse embryo. | an easy and rapid method of counting the number of cells in the preimplantation mouse embryo is described. the procedure increases the speed with which large numbers of embryos can be processed using a simple squash technique. cell numbers are determined by exposing the embryos to the fluorescent dna-binding dye, hoechst 33258, removing the zona pellucida and simply squashing the embryo and counting the number of fluorescent nuclei. an increase in fluorescent intensity and maintenance of nuclear ... | 1985 | 2412884 |
| expression of epidermal keratins and filaggrin during human fetal skin development. | the major structural proteins of epithelia, the keratins, and the keratin filament-associated protein, filaggrin, were analyzed in more than 50 samples of human embryonic and fetal skin by one-dimensional sds page and immunoblotting with monoclonal and polyclonal antibodies. companion samples were examined by immunohistochemistry and electron microscopy. based on structural characteristics of the epidermis, four periods of human epidermal development were identified. the first is the embryonic p ... | 1985 | 2413039 |
| genetics of insulin-specific helper and suppressor t cells in nonresponder mice. | the role of insulin-specific helper and suppressor t cells in the h-2-linked genetic control of antibody responses to heterologous insulins was examined in vitro. these data demonstrate that pork insulin stimulates both primed helper t cells and dominant suppressor t cells in all nonresponder strains tested. thus, the nonresponder phenotype is attributed to the activation of specific suppressor t cells rather than to an absence of helper t cell activity. examination of the antigenic cross-reacti ... | 1985 | 2413109 |
| characterization of an unique rna initiated immediately upstream from human alpha 1 globin gene in vivo and in vitro: polymerase ii-dependence, tissue specificity, and subcellular location. | we have identified an abundant transcript initiated upstream from the canonical cap site of human alpha 1 globin gene in bone marrow cells and in cos-7 cells transfected with an alpha 1 globin gene-containing plasmid. similar to the major alpha 1 globin transcript, this upstream rna is present almost exclusively in the cytoplasm of the transfected cos-7 cells. it is also synthesized efficiently in vitro by rna polymerase ii in the nuclear extracts prepared from a hela cell line and an erythroleu ... | 1985 | 2413422 |
| protective characteristics of the antigen common to pseudomonas and vibrio and significance of bacterial endotoxins in protection against selected bacterial infections. | the protective effect of different vaccines against the killing activity of pseudomonas aeruginosa and vibrio parahaemolyticus for mice was studied. cross experiments confirmed the high protective effect of the pseudomonas common antigen (original endotoxin protein) contained in pseudomonas vaccine, which, together with other soluble components, substantial afforded protection of mice against vibrios and thus confirmed the antigenic relationship between pseudomonas and vibrio. satisfactory resul ... | 1985 | 2413656 |
| syngeneic monoclonal antibody against melanoma antigen with interspecies cross-reactivity recognizes gm3, a prominent ganglioside of b16 melanoma. | it has previously been reported that a mouse (c57bl/6) monoclonal antibody, m2590, was established against syngeneic melanoma b16 cells, which was shown to react only with melanoma cells from various species but not with other tumor cells or normal tissues (taniguchi, m., and wakabayashi, s. (1984) gann 75, 418-426). in the present study, the specificity of m2590 antibody was shown to be directed to a saccharide arrangement (neuac alpha 2-3gal beta 1-4glc (or -glcnac)) of gangliosides by three d ... | 1985 | 2414279 |
| transcriptional and post-transcriptional regulation of l-type pyruvate kinase in diabetic rat liver by insulin and dietary fructose. | regulation of the expression of the hepatic l-type pyruvate kinase gene by insulin and dietary fructose was studied in diabetic rats. insulin increased the levels of putative nuclear rna precursor species of this enzyme in parallel with that of total cellular pyruvate kinase l mrna. these changes occurred more slowly than those induced by dietary fructose. insulin caused a 3-fold increase in transcription of the pyruvate kinase l gene after 6 h and a 6-fold increase after 16 h. the increase caus ... | 1985 | 2414297 |
| [vital staining of the conjunctiva for prevention of epithelial implantation cysts]. | selective vital staining with 1% toluidine blue makes short-term intraoperative differentiation of the conjunctiva from tenon's capsule possible. it is used with advantage in operations where the anatomical situation is unclear to avoid displacement of conjunctival epithelium into deeper tissues and subsequent growth of cysts. examples of its application are reoperations with excessive scars after squint or detachment surgery, wound dehiscence after enucleations, lacerated wounds after accidents ... | 1985 | 2415747 |
| differences in carbohydrate specificities and complement-activating capacity of guinea pig and human antibodies to neuraminidase-treated autologous erythrocytes. | guinea pig erythrocytes desialated by treatment with neuraminidase from vibrio cholerae were lyzed in autologous serum through a natural-antibody-dependent activation of the classical complement pathway. lysis was inhibited when a mannose, glucose, galactose or n-acetyl-glucosamine was added to the incubation mixture. methyl-alpha- or -beta-d-galactopyranosides were poorly effective and n-acetyl-d-galactosamine was not effective at all. inhibition of lysis by the carbohydrates was due neither to ... | 1985 | 2415814 |
| isolation and characterization of influenza c virus inhibitor in rat serum. | two hemagglutination inhibitors for influenza c virus were isolated from pooled sera of normal rats by sequential chromatography on blue sepharose cl 6b, ultrogel aca 22, and deae-cellulose. the two inhibitors were identified as alpha 1-macroglobulin and murinoglobulin by comparison with the authentic samples. these inhibitors abolished the hemagglutination by influenza c virus strains but did not affect the hemagglutination by influenza a and b virus strains. hemagglutination inhibition activit ... | 1985 | 2416143 |
| electrophoretic resolution of microheterogeneity in vibrio cholerae lipopolysaccharide. | lipopolysaccharide (lps) from vibrio cholerae has been analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. under normal conditions of electrophoresis which resolve escherichia coli lps, v. cholerae lps shows two diffuse and unresolved bands. however, on long gels at low concentration it can be resolved into two major band types. there are at least 10 slow moving, discrete bands of regular periodicity and three fast moving bands. comparison with lps from e. coli indicates that ... | 1985 | 2417638 |
| effect of carrier on the immunogenic capacity of synthetic cholera vaccine. | in the design of the synthetic antigens and synthetic vaccines, primary consideration should be given to the choice of the carrier. since small peptides which are being used as the relevant antigenic determinants are likely to be poor immunogens as such, the augmentation of their immunogenic capacity by the carrier or any other means is crucial for the induction of immunity. in the present study, we explored several approaches for the enhancement of the immune response towards synthetic peptides ... | 1985 | 2421153 |
| augmentation of natural killer activity by neuraminidase treatment of lymphocytes from tumor-bearing mice. | spleen cells from tumor-bearing mice showed decreased natural killer (nk) activity and decreased binding to target cells with progression of the tumor. treatment of spleen cells from tumor-bearing mice with vibrio cholerae neuraminidase (vcn) increased the cytotoxicity to a level twice or more as high as that of untreated cells, but the same treatment of spleen cells from normal mice had no or little effect. on the other hand, neither in spleen cells from tumor-bearing mice nor in those from nor ... | 1986 | 2421536 |
| m cell transport of vibrio cholerae from the intestinal lumen into peyer's patches: a mechanism for antigen sampling and for microbial transepithelial migration. | viable vibrio cholerae o1 were inoculated into the intestinal lumen of nonimmune rabbits. the vibrios were phagocytosed by m cells over peyer's patch lymphoid follicles, carried in vesicles through the epithelium, and discharged among underlying lymphocytes and macrophages. autoradiography of v. cholerae labeled with [2-3h]adenine confirmed transport. indigenous bacteria with and without capsules were also taken up from control loops and carried through m cells into peyer's patches. v. cholerae ... | 1986 | 2422297 |
| the response of the lung to foreign compounds that produce free radicals. | | 1986 | 2423026 |
| the interaction of myelin basic protein with tubulin and the inhibition of tubulin carboxypeptidase activity. | tubulin carboxypeptidase was found to be inhibited by myelin basic protein in a concentration dependent manner. the inhibition was produced by the interaction between myelin basic protein with the substrate. as a consequence of this interaction, turbid insoluble aggregates were formed at either 5 degrees or 37 degrees c. the turbidity increased by increasing the myelin basic protein concentration and it reached a plateau at a molar ratio of myelin basic protein to tubulin dimer of about 6. at pl ... | 1986 | 2423083 |
| on the origin and progression of human breast cancer. | the mature human female breast contains thousands of hormone-sensitive, potentially milk-producing microorgans, called lobules. in the nonpregnant state they vary in size from 1 to 8 mm but most are 1 to 2 mm in diameter. each lobule is drained by a terminal duct attached to the main duct system. it is called the terminal ductal-lobular unit, which normally regresses at menopause. most breast diseases except papillomas in major ducts arise in terminal ductal-lobular units. disease processes such ... | 1986 | 2424309 |
| molecular cloning and expression in escherichia coli k-12 of the o antigens of the inaba and ogawa serotypes of the vibrio cholerae o1 lipopolysaccharides and their potential for vaccine development. | the gene clusters that determine the biosynthesis of both the inaba and ogawa serotypes of the o antigen of the lipopolysaccharide of vibrio cholerae were cloned and expressed in escherichia coli k-12. restriction analysis of the clones demonstrated that about 15 kilobases were common to all clones and a further 5 kilobases were common to the ogawa clones. the o antigens expressed by e. coli k-12 had the specificity of v. cholerae. antibodies raised against e. coli k-12 that harbor one of these ... | 1986 | 2426198 |
| identification and characterization of the nmyc gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities. | increased n-myc (now designated nmyc in human gene nomenclature) gene expression has been detected at the transcriptional level in certain types of neoplasms. as yet, the n-myc gene product has not been identified. to detect and characterize the n-myc gene product, we have developed monoclonal antibodies against the putative n-myc gene product made in escherichia coli as a fusion protein. the antibodies that recognize the n-myc-specific regions were selected on the basis of their reactivities to ... | 1986 | 2426708 |
| evidence that ruthenium red disturbs the synaptic transmission in the rat hippocampal slices through interacting with sialic acid residues. | ruthenium red (rr) at a concentration of 0.71 mm selectively blocked synaptic transmission in hippocampal slices. antidromically evoked potentials and fibre potentials were only little affected. the action of rr was reversible by washout, but only following shorter (40-50 min) times of incubation. after longer incubation times (hours), the abolished population spike did not recover after washout but could be restored by facilitation of the calcium transport into the nerve terminal with 3,4-diami ... | 1986 | 2427153 |
| morphometric analysis of spermatozoa in the assessment of human male fertility. | a videomicrographic system was developed for measurement of morphometric parameters of human spermatozoa. contours of the images of spermatozoa on a video monitor are digitized by manually tracing them with the cursor of an electromagnetic digitizer integrated to a microcomputer. the accuracy and precision of the methodology were evaluated. a comparison of human sperm heads in shallow wet preparations and in dried, stained preparations indicated that the latter were smaller in length, width, pro ... | 1986 | 2427496 |
| oncogenes in human primary hepatic cancer. | transfection assay of nih 3t3 cells was performed with dnas isolated from ten human phc (primary hepatic cancer) specimens and a hepatoma 7402 cell line. positive results were obtained in 7402 and in six out of ten phc dnas. human n-ras gene was identified in transfectants from 7402 dna and transformed cells from three phc dna samples, which had passed more than two cycles of transfection. the expression of n-ras was also remarkably enhanced in six out of nine poly(a)+rna samples isolated from p ... | 1986 | 2427530 |
| oligodendroglia development in cell culture as monitored with a monoclonal antibody. | a new marker for young oligodendrocytes has been identified by a monoclonal antibody (mog-1, igm isotype) prepared from cerebellar plasma membrane stimulated mouse lymphocytes. mog-1 reactive cells in the mouse cerebellum first appear at day 19 of gestation. future white matter layers of fixed sections of neonatal rat cerebellum were labeled with mog-1. although em analysis has shown cell-surface binding by presumptive oligodendroglia in neonatal cerebellum, the antibody does not bind to compact ... | 1986 | 2427670 |
| the relationship between the keratocyst antigen (kca) and keratin. | the relationship of the keratocyst antigen (kca), the soluble component present in most keratocyst fluids, and keratin, was studied with immunofluorescence microscopy comparing their distribution in developing mouse embryonic teeth and in human ameloblastomas. in these tissues both molecules showed a strong codistribution in epithelial cells. in the embryonic teeth both molecules were present in the stratum intermedium cells between the stellate reticulum cells and ameloblasts, but the secretory ... | 1986 | 2427676 |
| the effect of removal of d-fructose on the antigenicity of the lipopolysaccharide from a rough mutant of vibrio cholerae ogawa. | the lipopolysaccharide (lps) of a rough mutant (95r) of vibrio cholerae ogawa has been investigated chemically and serologically. d-fructose was released from lps under conditions (10mm trifluoroacetic acid, 60 degrees, 1 h) that liberated no other sugar constituent of the lps (2-amino-2-deoxy-d-glucose, d-glucose, l-glycero-d-manno-heptose). upon periodate oxidation, d-fructose and d-glucose were oxidised quantitatively, whereas approximately 50% of heptose was periodate-resistant. the data ind ... | 1986 | 2428494 |
| structural studies of the o-antigen from vibrio cholerae o:21. | the o-antigen from vibrio cholerae o:21 has been investigated, using n.m.r. spectroscopy, methylation analysis, and smith degradation as the main methods. it is concluded that the o-antigen is composed of tetrasaccharide repeating-units having the following structure (in which hep = d-glycero-d-manno-heptose). (formula: see text). | 1986 | 2428496 |
| [methodological aspects of the study of bacterial chemotaxis]. | | 1986 | 2429040 |
| enhancement of histamine release from human basophils pretreated with different sialidases. | histamine release from human basophils was investigated in vitro after removal of cell membrane sialic acid by three different sialidases. pretreatment of the cells with sialidases from cl. perfringens, v. cholera or influenza virus a2 enhanced histamine release induced by subsequent stimulation of the cells with anti-ige or the plant lectin concanavalin a and caused a shift to the left of the dose-response curve for anti-ige. the enhanced histamine release was reflected in a increased calcium s ... | 1986 | 2429528 |
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |
| aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia. | we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ... | 1986 | 2430056 |
| a ganglioside species (gd1 alpha) migrates at a slow rate and cmp-sialic acid severalfold faster in xenopus sciatic nerve: fluorographic demonstration. | the ninth dorsal root ganglion of adult xenopus laevis was labeled with n-acetyl-d-[6-3h]mannosamine, and intraaxonal migration of gangliosides was examined by analysis of the chloroform/methanol extract of each of 5-mm consecutive nerve segments by tlc coupled with fluorography. a unique disialoganglioside (gd1 alpha), which amounted to up to 83% of the total ganglioside in this nerve, migrated at 1-2 mm/day at 15 degrees c. this contrasts with the rapid transport of other ganglioside species p ... | 1986 | 2430059 |
| effect of nutrient deprivation on lipid, carbohydrate, dna, rna, and protein levels in vibrio cholerae. | the response of vibrio cholerae to low nutrient levels was determined by measuring the concentrations of lipids, carbohydrates, dna, rna, and proteins over a 30-day starvation period. ultrastructural integrity was observed by transmission electron microscopy. total lipids and carbohydrates declined rapidly within the first 7 days, while dna and protein exhibited a more constant decline over the 30 days of starvation. in contrast, rna showed little decrease upon starvation. although neutral lipid ... | 1986 | 2430523 |
| [effects of enzymatic deglycosylation of human goiter thyroglobulin on its immunochemical properties]. | thyroglobulin (tg), isolated from soluble iodoproteins by ammonium sulphate fractionation, was enzymatically deglycosylated in vitro and analyzed by polyacrylamide gel electrophoresis, double immunodiffusion and non-commercial ria. carbohydrate and iodine content was chemically determined. by paage deglycosylated tg (dtg) showed the appearance of a major band in the 12s region and three slower migrating bands corresponding to higher aggregates than 19s tg. in immunodiffusion by testing native an ... | 1986 | 2430684 |
| the surface charge of toxoplasma gondii: a cytochemical and electrophoretic study. | the surface charge of toxoplasma gondii tachyzoites was evaluated by means of binding of colloidal iron hydroxide particles at ph 1.8, cationized ferritin particles at ph 7.2 and ruthenium red to the cell surface, as visualized by electron microscopy and by direct measurements of the electrophoretic mobility (epm) of the cells suspended in solutions of different ionic strength and ph. at ph 7.2, t. gondii has a negative surface charge with a mean epm of--1.1272 +/- 0.0917 micron.s-1 x v-1 x cm. ... | 1986 | 2431157 |