| [ms-elisa for detection of serum antibody level in leprosy patient--establishment of ms-elisa]. | | 1988 | 2978406 |
| defective monocyte function in patients with systemic lupus erythematosus. | peripheral blood adherent cells from patients with systemic lupus erythematosus (sle) were shown to have markedly reduced phagocytic activity as compared to normal adherent cells or those from non-sle patients receiving corticosteroid therapy. both resting and phagocytosing monocytes showed decreased hexose monophosphate shunt and glycolytic activity. mononuclear cells from sle patients showed grossly impaired proliferative activity after naio4 activation. furthermore, addition of sle adherent c ... | 1985 | 2981168 |
| neurological manifestations of the acquired immunodeficiency syndrome (aids): experience at ucsf and review of the literature. | in this review of the acquired immunodeficiency syndrome (aids), the authors have evaluated a total of 352 homosexual patients with aids or generalized lymphadenopathy managed at the university of california, san francisco (ucsf), between 1979 and 1984. of an initial unselected group of 318 patients, 124 (39%) were neurologically symptomatic, and one-third already had their neurological complaints at the time of presentation. an additional 210 aids patients with neurological symptoms have been r ... | 1985 | 2983051 |
| dna restriction endonuclease analysis of mycobacterium bovis and other members of the tuberculosis complex. | dna preparations from 24 new zealand isolates, two reference strains of mycobacterium bovis, and one reference strain each of mycobacterium microti, mycobacterium africanum, and mycobacterium tuberculosis were characterized by restriction endonuclease analysis. twenty-five restriction enzymes were investigated. the clearest differences in m. bovis patterns were obtained with the enzymes bsteii and bcli. these produced four and five different patterns, respectively, for the 24 local isolates. whe ... | 1985 | 2985647 |
| evidence for the generation of active oxygen by isoniazid treatment of extracts of mycobacterium tuberculosis h37ra. | crude extracts of mycobacterium tuberculosis h37ra, an isonicotinic acid hydrazide (isoniazid) (inh)-susceptible strain which has peroxidase activity, catalyzed the production of catechol from phenol in the presence of inh and h2o2 as shown by the development of the 444-nm absorption peak of oxidized catechol product. extracts of the inh-resistant strain of m. tuberculosis h37ra, which has no peroxidase, did not catalyze the reaction. the rate of development of the 444-nm peak increased proporti ... | 1985 | 2986545 |
| the influence of immunosuppression and immunodeficiency on infections with leprosy and tuberculosis. | | 1985 | 2987376 |
| bacteriological bases of short-course chemotherapy. | | 1985 | 2999945 |
| mechanisms of action of antituberculosis drugs in standard and short-course chemotherapy regimens. | | 1985 | 2999946 |
| modern short-course regimens in the treatment of tuberculosis. | | 1985 | 2999948 |
| [l forms of mycobacterium tuberculosis and their clinico-epidemiological significance]. | | 1985 | 3001898 |
| vitamin d3, gamma interferon, and control of proliferation of mycobacterium tuberculosis by human monocytes. | previous studies have shown that recombinant interferon gamma (ifn-gamma), crude t cell supernatants, or appropriate t-cell lines can cause total inhibition of the growth of m. tuberculosis inside murine peritoneal macrophages. in similar experiments with human monocytes much smaller effects are seen. this could be due to the relative immaturity of these cells. because dihydroxy vitamin d3 (1,25-(oh)2 d3) can cause phenotypic differentiation of immature leukemic lines into macrophage-like cells, ... | 1986 | 3002968 |
| clinical investigations of lymphadenopathy, including lymph node biopsies, in 24 homosexual men with antibodies to the human t-cell lymphotropic virus type iii (htlv-iii). | the findings of 27 lymph node biopsies performed on 24 homosexual patients with lymphadenopathy are presented. six had acquired immune deficiency syndrome (aids) and 18 lymphadenopathy only, of whom one subsequently developed aids. all these patients had antibodies to the human t-cell lymphotropic virus type iii (htlv-iii) suggesting that htlv-iii is currently the commonest cause of lymphadenopathy in homosexual men. the histopathological findings of six of seven nodes from aids patients showed ... | 1986 | 3004630 |
| genes for the protein antigens of the tuberculosis and leprosy bacilli. | the lambda gt 11 expression vector permitted us to survey protein antigens of mycobacterium leprae and mycobacterium tuberculosis expressed in escherichia coli. using monoclonal antibodies, recombinant clones were detected producing three major antigens of m. tuberculosis and five major protein antigens of m. leprae. these recombinant antigens produced in e. coli should prove useful for diagnosis, epidemiology and possibly the development of recombinant mycobacterial vaccines. | 1985 | 3006821 |
| macrophage activation by trehalose dimycolate requirement for an expression signal in vitro for antitumoral activity; biochemical markers distinguishing primed and fully activated macrophages. | it was possible to define the effects of trehalose dimycolate (tdm), a glycolipid extracted from mycobacterium tuberculosis, on mouse peritoneal macrophages more precisely using endotoxin-free culture conditions. tdm-elicited macrophages, when assayed in vitro in the absence of endotoxin, were unable to limit tumor growth; however, after a short treatment (4 h) with low doses of lipopolysaccharide (lps; 1-10 ng/ml), they exhibited a strong cytostatic capacity against p815 mastocytoma cells. thus ... | 1986 | 3009201 |
| isolation and restriction endonuclease analysis of mycobacterial dna. | a method for the isolation of dna from mycobacteria propagated in vitro is described that utilizes organic solvents to extract lipoidal components from the outer membrane, and digestion with a protease (nagarse) and lysozyme to penetrate the cell wall. the mycobacterial cells were lysed by the addition of detergent and the dna was purified by digestion with pronase, sequential phenol and chloroform extractions, and digestion with rnaase a. the isolated dna, which was obtained in good yields, was ... | 1986 | 3011965 |
| [current bacteriological diagnosis of tuberculosis. i. the efficiency of bacteriological methods in identifying sources of infection]. | | 1985 | 3012749 |
| genetic relatedness among strains of the mycobacterium tuberculosis complex. analysis of restriction fragment heterogeneity using cloned dna probes. | chromosomal dna isolated from 3 reference and 2 clinical isolates of mycobacterium tuberculosis, 2 reference strains each of m. bovis and m. bovis bcg, and 1 reference strain of m. kansasii, was digested with 9 restriction endonucleases. the restriction fragments were analyzed by agarose gel electrophoresis. with each enzyme tested the patterns of the strains of m. tuberculosis, m. bovis, and m. bovis bcg were indistinguishable but clearly distinct from those of m. kansasii. a library of m. tube ... | 1986 | 3013055 |
| mycobacteria possess a surprisingly small number of ribosomal rna genes in relation to the size of their genome. | dnas from mycobacterium tuberculosis, m. intracellulare, m. phlei and m. smegmatis were digested by restriction enzymes and hybridized with three probes consisting of the 5' (16s rrna), the middle (16s and 23s rrna), and the 3' (23s and 5s rrna) portions of the escherichia coli rrnb operon. the resulting hybridization patterns indicate that slow-growing mycobacteria species (i.e., m. tuberculosis and m. intracellulare), with genome size 3.13 - 4.29 x 10(9) daltons, appear to possess only one rrn ... | 1986 | 3013168 |
| secondary pulmonary alveolar proteinosis occurring in two patients with acquired immune deficiency syndrome. | this report describes two patients with acquired immune deficiency syndrome (aids) in whom respiratory failure and opportunistic infection associated with secondary alveolar proteinosis developed. in one patient, the alveolar proteinosis was apparently secondary to mycobacterium tuberculosis and in the other to pneumocystis carinii and cytomegalovirus infection. both patients died of respiratory failure, and it was suspected that secondary alveolar proteinosis could have been a contributing caus ... | 1986 | 3014876 |
| natural mycobacteriostatic activity in human monocyte-derived adherent cells. | the effects of human monocyte maturity on the replication of virulent mycobacterium tuberculosis were examined. mycobacteria grew readily in freshly isolated, adherent peripheral blood monocytes and in monocyte-derived macrophages obtained after 7 days in culture, as measured by counts of acid-fast bacilli and colony-forming units. monocytes cultured for only 3 days before infection, however, were less permissive for the mycobacteria than either uncultured or 7-day cells. the association between ... | 1986 | 3014936 |
| restriction fragment analysis of chromosomal dna defines different strains of mycobacterium tuberculosis. | as an initial step in gaining a better understanding of the important clinical properties that vary between strains of mycobacteria, we attempted to find molecular markers that would define different strains of mycobacterium tuberculosis. we used restriction fragment analysis with the endonuclease mboi and hybridization with total m. tuberculosis dna to examine dna differences between 15 strains of m. tuberculosis. we were able to identify different strains using this method. in order to assess ... | 1986 | 3017166 |
| cloning and expression of mycobacterium bovis bcg dna in "streptomyces lividans". | the ability of "streptomyces lividans" to use the expression signals of genes from mycobacterium bovis bcg was tested in vivo by using gene fusions. random dna fragments from m. bovis bcg were inserted into promoter-probe plasmids in escherichia coli and in "s. lividans." comparison with promoter activity detected with random dna fragments from the respective hosts suggested that "s. lividans" efficiently utilizes a high proportion of mycobacterial promoters, whereas a smaller fraction are expre ... | 1986 | 3020007 |
| paediatric meningitis in the western cape. a 3-year hospital-based prospective survey. | between july 1981 and june 1984 1223 cases of meningitis were seen in the department of paediatrics, tygerberg hospital. the commonest form in each population group was aseptic meningitis. positive viral cultures were obtained from the csf in 108 cases. the median age of white children with aseptic meningitis, 64 months, was significantly greater than that of coloured children, 45 months (p greater than 0.0001), and black children, 26 months (p greater than 0.014). the commonest cause of confirm ... | 1986 | 3020718 |
| variability of mycobacterial population in the process of the experimental chemotherapy of tuberculosis. | | 1986 | 3027864 |
| the significance of l-forms of mycobacteria in the clinical picture of tuberculosis. | | 1986 | 3027865 |
| [bacteriologic data on the isolation of koch's bacillus from cases of extrapulmonary tuberculosis over an 8-year period]. | | 1986 | 3027871 |
| the 65-kilodalton antigen of mycobacterium tuberculosis. | the immune response of the host to the antigens of mycobacterium tuberculosis plays the key role in determining immunity from infection with as well as the pathogenicity of this organism. a 65-kilodalton (kda) protein has been identified as one of the medically important antigens of m. tuberculosis. the gene encoding this antigen was isolated from a lambda gt11-m. tuberculosis recombinant dna library using monoclonal antibodies directed against the 65-kda antigen as the specific probes. the nucl ... | 1987 | 3029018 |
| comparison of four beta-lactamase inhibitors in combination with ampicillin against mycobacterium tuberculosis. | the in-vitro activity of a beta-lactamase inhibitor (clavulanic acid, sulbactam, bl-p2013 or bl-p2090) in combination with ampicillin against 13 isolates of mycobacterium tuberculosis was determined by broth dilution. the agents were tested in 1:1 molar ratio of ampicillin to beta-lactamase inhibitor. the beta-lactamase inhibitors alone did not inhibit growth at the highest concentration tested. the mic90 for ampicillin alone was greater than 32 mg/l. clavulanic acid plus ampicillin was the most ... | 1987 | 3030999 |
| [diagnosis and treatment of urinary and genital tuberculosis in men]. | | 1987 | 3033805 |
| [bacteriological diagnosis of intestinal tuberculosis]. | | 1987 | 3033807 |
| epstein-barr virus-transformed b cells process and present mycobacterium tuberculosis particulate antigens to t-cell clones. | we have analyzed the presentation of mycobacterial antigens by epstein-barr virus-transformed human b (ebv-b) cells to mycobacteria-specific t-cell clones and lines, and to purified resting t cells. ebv-b cells were able to process and present not only soluble forms of antigen, such as ppd and the expressate preparation of m. tuberculosis strain h37rv, but also particulate forms of antigen, such as whole mycobacterial h37rv or m. bovis organisms. electron microscopy studies demonstrated the capa ... | 1987 | 3036374 |
| comparison of bronchial washing, brushing and biopsy for diagnosis of pulmonary tuberculosis. | the diagnostic yields of bronchial washings, bronchial brushings and lung biopsy specimens were compared in 50 patients with positive mycobacterium tuberculosis cultures. the number of positive results obtained with cultures of bronchial brushings was significantly higher than that with bronchial washings (p less than 0.001). the histological study of biopsy lung material improved the rate of immediate or rapid diagnosis of tuberculosis (p less than 0.001). | 1987 | 3036502 |
| isolation and restriction site maps of the genes encoding five mycobacterium tuberculosis proteins. | a series of recombinant phage expressing five mycobacterium tuberculosis antigens were isolated. restriction maps for these antigens were deduced, and the size of the expressed proteins was determined. | 1987 | 3036711 |
| rifabutin (ansamycin lm 427): a new rifamycin-s derivative for the treatment of mycobacterial diseases. | rifabutin (ansamycin lm 427), a semisynthetic spiropiperidyl derivative of rifamycin s, shows good in vitro activity against most mycobacterial species, including mycobacterium avium complex. in animal models, the drug is more active against both mycobacterium tuberculosis and mycobacterium leprae than in rifampin, and studies indicate that rifabutin is active against some rifampin-resistant strains of both species. the drug has a long half-life (16 hr) in humans and a marked tissue tropism, wit ... | 1987 | 3037676 |
| opportunistic infections in the acquired immune deficiency syndrome. | the acquired immune deficiency syndrome (aids) results in a t lymphocyte deficiency and, consequently, a susceptibility to opportunistic infections with organisms that previously were better known as causes of disease in patients with hodgkin's disease or recipients of immunosuppressive therapy. pneumocystis carinii, candida albicans, and cytomegalovirus (cmv) are among the most frequently observed pathogens in aids patients, followed by atypical mycobacteria, toxoplasma gondii, mycobacterium tu ... | 1987 | 3037706 |
| evidence for activation of a respiratory burst in the interaction of human neutrophils with mycobacterium tuberculosis. | we examined the capacity of human neutrophils to develop a respiratory burst, as monitored by superoxide release, in response to interaction with mycobacterium tuberculosis. serum-opsonized, heat-killed mycobacteria induced significant release of superoxide from neutrophils after 30 min of exposure, with a maximum release of 34 +/- 1.7 nmol/30 min per 5 x 10(6) neutrophils occurring with a mycobacterium/neutrophil ratio of 40:1. similar levels of superoxide release were induced by live mycobacte ... | 1987 | 3040594 |
| intrathoracic lymphadenopathy in postprimary tuberculosis. | in the past, hilar or mediastinal lymphadenopathy was considered by many to be a feature of only the primary or first infection with mycobacterium tuberculosis, and to exclude the diagnosis of reactivation or postprimary tuberculosis. in a series of 56 adult patients with documented postprimary disease due to m tuberculosis, we found hilar or mediastinal lymphadenopathy in three cases (5%). although intrathoracic lymphadenopathy was more common in primary tuberculosis, we do not believe that int ... | 1988 | 3043694 |
| pulmonary effects of aids: nosocomial transmission. | this review provides an overview of the risk of nosocomial infection in the "aids era." airborne spread of mycobacterium tuberculosis from affected patients has re-emerged as a hazard to hospital personnel. the risk of acquiring clinical illness due to pneumocystis carinii or cytomegalovirus is, in contrast, a function of the immunocompetence of the health care worker. methods of transmission as well as the epidemiology of human immunodeficiency virus-related infection in the health care worker ... | 1988 | 3044687 |
| the role of colonoscopy in the diagnosis of tuberculosis. | the value of colonoscopy in the diagnosis of ileocolonic tuberculosis at groote schuur hospital was assessed and the published world experience reviewed. during a 4-year period, abdominal tuberculosis was diagnosed in 94 patients, of whom 18 manifested involvement of the ileocecal area and/or colon. colonoscopy, performed in 10 of these, provided diagnostic information in 7. since the endoscopic appearance of diseased mucosa is nonspecific, adequate bacteriological and histological assessment of ... | 1988 | 3053873 |
| epidural spinal infection in intravenous drug abusers. | the incidence of spinal epidural abscess and disk space infection appears to be rising in intravenous drug-using patients. we report 18 cases seen over three years in two municipal hospitals. staphylococcus aureus was the most common infective agent, but two patients had mycobacterium tuberculosis infection. computed tomography of the spine facilitates diagnosis. early treatment improves outcome but host factors play a role in recovery. | 1988 | 3058095 |
| tuberculous lymphadenopathy: a collective review with a case report. | a collective review of tuberculous lymphadenopathy is presented with respect to incidence, pathogenesis, clinical manifestations, diagnosis, differential diagnosis, and treatment. a typical case report is also presented. a thorough history and physical examination, a purified protein derivative (ppd) skin test and acid-fast bacillus (afb) stains, and histopathology of the node may provide useful information. positive culture results are the only means of confirming the diagnosis and for distingu ... | 1988 | 3074173 |
| crossprotection against nontuberculous mycobacterial infections by mycobacterium tuberculosis memory immune t lymphocytes. | adoptive immunization of t cell-deficient recipient mice with m. tuberculosis-specific memory immune t lymphocytes conferred upon these animals the ability to express significantly enhanced resistance both to the homologous infection, and to three strains of nontuberculous mycobacteria. these results support the hypothesis, therefore, that antigenic determinants possessed by the four mycobacterial strains that are relevant to the generation of protective cellular immunity are identical or closel ... | 1986 | 3079814 |
| diagnosis of tuberculosis by detection of mycobacterial antigen in pleural effusions and ascites. | we describe a method for the diagnosis of pleural and peritoneal tuberculosis by the detection of tuberculous antigens using an enzyme-linked immunosorbent assay. eleven tuberculous pleural fluid and 10 tuberculous ascitic fluid samples were studied by this technique, using 10 non-tuberculous pleural fluid and 14 non-tuberculous ascitic fluid samples as controls. an absorbance value of 0.3 was found to separate the tuberculous groups from their controls to a statistically significant extent (asc ... | 1986 | 3079923 |
| primary resistance to the major anti-tuberculosis drugs in ethiopia. | | 1986 | 3080312 |
| generation of cytotoxic t lymphocytes from thymocyte precursors to trinitrophenyl-modified self antigens. i. requirement of both killer-helper factor(s) and interleukin 2 for ctl generation from a subpopulation of thymocytes. | the requirement for signals in the induction of cytotoxic t lymphocytes (ctl) from thymocyte precursors has been investigated. either unfractionated or peanut agglutinin-binding (pna+) c3h/he thymocytes were stimulated with mitomycin c(mmc)-treated, 2,4,6-trinitrophenyl(tnp)-modified syngeneic spleen cells in the presence of a variety of lymphokine preparations. cellfree supernatant (cfs) from purified protein derivatives(ppd-cfs) stimulated mycobacterium tuberculosis (tbc)-primed cells, or part ... | 1986 | 3080519 |
| tuberculosis--united states, 1985--and the possible impact of human t-lymphotropic virus type iii/lymphadenopathy-associated virus infection. | data on the number of reported cases of tuberculosis in the us in 1985 support the hypothesis that human t-lymphotropic virus type iii/lymphadenopathy-associated virus (htlv-iii/lav) infection of persons infected with the tubercle bacillus has caused an increase in tuberculosis in some areas. the 21,801 tuberculosis cases reported in 1985 represented a 2% decline from the 1984 total of 22,255 cases. however, the average decline in the 1982-84 period was 6.7%, indicating a slowing of the downwa ... | 1986 | 3080659 |
| preventive therapy with isoniazid. cost-effectiveness of different durations of therapy. | daily administration of isoniazid for one year to persons infected with mycobacterium tuberculosis is effective in considerably reducing the risk of disease. from a practical viewpoint, this approach to prevention is less than ideal because it results in considerable costs as health care providers monitor for possible hepatotoxic effects and because it is difficult to maintain compliance for 12 months. the efficacy and toxicity of isoniazid preventive therapy regimens of 12, 24, and 52 weeks' du ... | 1986 | 3081740 |
| responsiveness to live m. tuberculosis, and common antigens, of sonicate-stimulated t cell lines from normal donors. | live mycobacteria are more efficient vaccines against mycobacterial disease than killed ones. a possible explanation is the existence of important protective antigens released by live bacilli, which are not present in any significant quantity in dead ones. conversely, internal mycobacterial antigens may be irrelevant to protection if not released by live bacilli. we show here, using t cell lines derived by limiting dilution from the peripheral blood of normal donors stimulated with sonicated bcg ... | 1986 | 3082544 |
| cell mediated immunity cross-reactions of mycobacteria: polymorphism of target bacterial antigens. | swiss white mice were immunized with different mycobacteria and delayed type hypersensitivity (dth) responses were studied by the foot-pad swelling technique of gray and jennings (1955). extensive cross-reactions in dth, outside the limits of runyon's groups were observed. as a general trend slow growing mycobacteria showed greater cross-reactivity with slow growers than with rapid growers and vice versa. the implied cross-protective significance of dth cross-reactions was further confirmed by d ... | 1986 | 3082545 |
| inhibition of mitogenesis induced by phytohemagglutinin and lens culinaris lectin in adherent-cell supernatants treated with protein extract of mycobacterium tuberculosis. | specific stimulation of t cells by phytohemagglutinin and lens culinaris lectin was inhibited by a soluble factor(s) secreted by normal adherent cells stimulated with culture filtrate protein extract (cfpe) derived from bacterial cultures of mycobacterium tuberculosis h37ra (avirulent) and h37rv (virulent). the induction of the inhibitory factor was blocked by the presence of hyperimmune antisera to h37rv or h37ra cfpe. the inhibitory factor did not seem to be a cfpe reprocessed by the adherent ... | 1986 | 3082760 |
| comparison between tuberculoproteins a and c in the serodiagnosis of tuberculosis by enzyme-linked immunosorbent assay. | tuberculoproteins a and c obtained from culture filtrate of mycobacterium tuberculosis by alcohol fractionation were utilized in an enzyme-linked immunosorbent assay to measure the amount of reactive igg in the sera from advanced and moderately advanced tuberculosis patients, leprosy patients and normal individuals. the a-protein detected significant levels of igg in the sera from both advanced and moderately-advanced tuberculosis patients and patients with leprosy as compared to the normal grou ... | 1986 | 3082768 |
| effect of relative centrifugal force and centrifugation time on sedimentation of mycobacteria in clinical specimens. | optimum relative centrifugal force (rcf) and centrifugation time to concentrate mycobacteria in clinical specimens were determined by processing split samples of sputa and urines containing mycobacteria with combinations of different rcfs and centrifugation times. although individual test results showed considerable variation in the recovery rates of mycobacteria in the sediment, the data indicated that higher recovery rates occurred as centrifugation speed and time were increased. with a 15- to ... | 1986 | 3082926 |
| [value of microscopic detection of acid fast rods in tuberculosis of the middle ear]. | the microscopic identification of acid-proof rod-shaped bacilli via smear is of great importance for the diagnosis of tuberculous otitis media, since it is performed quickly and easily, for it enables an early diagnosis and treatment. the importance of the staining method using auramine is discussed, and it is examined if contamination by apathogenic or atypic mycobacteriae can yield a wrong positive result. | 1986 | 3083165 |
| the purification and biological activity of a macrophage-derived factor with interleukin 1-like activities from guinea pigs. | a macrophage-derived factor with interleukin 1-like activity was purified from culture supernatant of muramyl dipeptide-stimulated peritoneal exudate macrophages of guinea pigs. starting with serum-free culture supernatant, the purification was carried out by gel permeation chromatography, affinity chromatography on procion red agarose, removal of carry-over serum proteins by sepharose-coupled antibodies against bovine serum proteins, anion exchange chromatography and hydrophobic chromatography. ... | 1986 | 3083870 |
| evaluation of streptomycin and ethambutol concentrations for susceptibility testing of mycobacterium tuberculosis by radiometric and conventional procedures. | clinical isolates of mycobacterium tuberculosis were used to compare various concentrations of streptomycin and ethambutol in the bactec 460 (johnston laboratories, inc., towson, md.) radiometric method for drug susceptibility testing with those in the conventional method. streptomycin used at 2.0 micrograms/ml for both methods showed a 0.99 agreement with susceptible strains and a 0.97 agreement with resistant strains. ethambutol used at 2.5 micrograms/ml for the radiometric method showed 1.00 ... | 1986 | 3084552 |
| analysis of mycolic acid cleavage products and cellular fatty acids of mycobacterium species by capillary gas chromatography. | after growth and experimental conditions were established, the mycolic acid cleavage products, constituent fatty acids, and alcohols of representative strains of mycobacterium tuberculosis, m. smegmatis, m. fortuitum complex, m. kansasii, m. gordonae, and m. avium complex were determined by capillary gas chromatography. reproducible cleavage of mycolic acid methyl esters to tetracosanoic (24:0) or hexacosanoic (26:0) acid methyl esters was achieved by heating the sample in a high-temperature muf ... | 1986 | 3084554 |
| tuberculous meningitis--clinical and laboratory review of 100 patients. | in developing countries tuberculous meningitis is a difficult infection to differentiate from other central nervous system (cns) infections. this paper presents the history, physical findings, laboratory data, and clinical course of 100 patients who were admitted to a special ward and had csf cultures positive for mycobacterium tuberculosis. fifty-four patients were comatose when admitted and 76 had meningeal signs. mean admission csf values were wbc 531, glucose 23 mg/dl, and protein 166 mg/dl. ... | 1986 | 3084628 |
| tuberculosis of peripheral joints: an often missed diagnosis. | eleven elderly patients with tuberculous arthritis of 12 peripheral joints were studied retrospectively. the disease usually presented as a monoarticular arthritis often in the absence of other signs of tuberculosis. a delay in diagnosis was a common feature and synovial biopsies provided the highest diagnostic yield. | 1986 | 3084774 |
| phenotypic changes in mycobacteria grown in oxygen-limited conditions. | laboratory strains of mycobacterium phlei, m. smegmatis, m. fortuitum, m. gordonae, m. kansasi, m. bovis, m. tuberculosis and m. intracellulare were adapted to grow in an anaerobic environment. concomitant with the transition to anaerobic growth was loss of acid-fastness, loss or modification of colonial pigmentation, and loss of ability to grow on a malachite green-containing medium. the mycobacteria grown anaerobically produced acid from a greater range of carbohydrates than aerobically grown ... | 1986 | 3084791 |
| detection of antibodies to mycobacterium tuberculosis plasma membrane antigen by enzyme-linked immunosorbent assay. | antibody activity against mycobacterium tuberculosis of sera from an area with a high prevalence of tuberculosis was measured by enzyme-linked immunosorbent assay (elisa) with a plasma-membrane extract from m. tuberculosis strain h37rv. all sera from relapsed tuberculosis patients and 82.5% of sera from new untreated cases gave positive results. the seronegative group of tuberculosis patients gave positive results by direct microscopy and culture. no clear correlation between antibody and delaye ... | 1986 | 3084792 |
| a combined detection & drug susceptibility testing of mycobacterium tuberculosis in smear positive sputum samples using biphasic vial radiometric method. | | 1986 | 3086224 |
| immunochemical characterization of saline-extracted antigens of mycobacterium tuberculosis h37ra. | the saline extract (se) of mycobacterium tuberculosis h37ra yielded four major fractions on sephadex g-100, with average molecular weights of 48,000 (fraction i, f i) 32,000 (fraction ii, f ii), 15,000 (fraction iii, f iii) and 5,000 (fraction iv, f iv). fi and fii gave single bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, showed complete immunological cross-reactivity with each other and were more potent in reacting with antibodies in patients' (tuberculosis) sera than soni ... | 1986 | 3086238 |
| determination of mycobacterial antigens in sputum by enzyme immunoassay. | an enzyme-linked immunosorbent assay (elisa) was examined for its usefulness in detecting mycobacterial antigens in sputum. a double-antibody sandwich procedure was set up by using a commercially available hyperimmune serum directed against mycobacterium bovis, bcg. the elisa was able to detect 10 ng of protein per ml of bcg sonic extract. the system also clearly distinguished mycobacterium tuberculosis organisms from mycobacterium avium and mycobacterium kansasii organisms. a total of 68 unknow ... | 1986 | 3086369 |
| sensitivity and specificity of enzyme-linked immunosorbent assay in the detection of antigen in tuberculous meningitis cerebrospinal fluids. | a sandwich enzyme-linked immunosorbent assay was developed for its potential utility in the detection of antigen in the cerebrospinal fluid of patients with tuberculous meningitis. cerebrospinal fluids examined included those from untreated (group ia) and treated (group ib) mycobacterium tuberculosis meningitis, nonseptic central nervous conditions (group ii) such as epilepsy, viral meningitis, and tetany, and nonmycobacterial septic meningitis (group iii). the average levels of antigens determi ... | 1986 | 3086370 |
| nonweekend schedule for bactec drug susceptibility testing of mycobacterium tuberculosis. | determination of the drug susceptibility of mycobacterium tuberculosis by conventional methods using an agar-based medium may take 3 weeks or more to complete. on the other hand, results on positive cultures are generally available in 4 to 7 days with the radiometric (bactec, johnston laboratories, towson, md.) procedure. one disadvantage to the latter is the requirement to determine the quantity of 14co2 in each test vial on a daily basis from the day of inoculation. growth index readings often ... | 1986 | 3086371 |
| immunity to leprosy. iii. the in vitro induction of b lymphocyte proliferation by mycobacteria. | the development of murine proliferative response assays has been initiated to begin to evaluate t-lymphocyte responses to the antigens of mycobacterium leprae. in this study, m. leprae and 13 related strains of mycobacteria have been tested for stimulatory effects in proliferation assays using murine spleen, thymus or lymph node cultures. a number of mycobacteria were found to directly stimulate the proliferation of spleen and lymph node cells of all mouse strains tested including c3h/hej mice. ... | 1986 | 3086467 |
| lack of mycobacterium leprae-specific uptake in schwann cells. | among mycobacteria, mycobacterium leprae have a unique property to infect peripheral nerves, which is the cause of a variety of debilities seen in leprosy. the possibility of selective uptake of m. leprae by schwann cells was studied using a rat schwannoma cell line 33b and rat sciatic nerve-derived schwann cells. m. leprae were phagocytosed by 33b cells but so also were seven other mycobacteria ("mycobacterium w," bcg, m. tuberculosis h37rv, m. nonchromogenicum, m. vaccae, icrc bacillus, and m. ... | 1986 | 3086468 |
| physiological characteristics of the mycobacterium tuberculosis-m. bovis group of organisms with particular reference to heterogeneity within m. bovis. | by examining the abilities of mycobacterial strains to remove amino acids from solution, differences were found between mycobacterium tuberculosis and other closely related taxa. heterogeneity was observed in most taxa. strains of m. bovis were examined in greater detail. by using the amino acid typing method in combination with tests routinely used for differentiating mycobacterial species, and sds-polyacrylamide gel electrophoresis of whole cell soluble proteins, a great degree of heterogeneit ... | 1986 | 3086497 |
| [tuberculosis in patients with malignant neoplasms]. | out of 810 patients autopsied at our institute between 1972 and 1985, tuberculosis was demonstrated in 12 (1.5%), all between 40 and 80 years of age, regardless of cancer type. of 4,272 patients who underwent surgery for malignant neoplasms, 481 were examined for m. tuberculosis by culture in different specimens. all of eight positive cases were with sputum or bronchial mucus specimens, regardless of the site of primary cancer. tuberculosis complicating malignant thymoma or hodgkin's disease was ... | 1986 | 3086596 |
| [analysis of pulmonary tuberculomas diagnosed by exploratory excision]. | | 1986 | 3086609 |
| mycobacterium tuberculosis meningitis: a report of twelve cases and a literature review. | twelve recent cases of mycobacterium tuberculosis meningitis were presented, and the literature was reviewed. there are no particularly new or unique therapies or approaches to the management of this most serious disease. the major obstacle to successful diagnosis and treatment of tuberculous meningitis continues to be a lack of clinical suspicion of its presence. as illustrated in the cases presented, it has been our experience that patients already moribund or nonresponsive do not respond, reg ... | 1986 | 3086768 |
| effect of dose formulation on isoniazid absorption in two young children. | in an 8-month-old infant with tuberculous meningitis treatment with isoniazid was unsuccessful and was associated with lower than expected plasma concentrations of isoniazid (measured concentration 0.1 microgram/ml). the infant had received isoniazid as a crushed tablet admixed with apple sauce. oral administration of the parenteral solution of isoniazid (nydrazid, squibb) mixed in apple juice produced a higher isoniazid concentration (2.9 micrograms/ml) and the child improved clinically. pharma ... | 1986 | 3086830 |
| [bacteriological examination of the urine for detection of urogenital tuberculosis]. | | 1986 | 3086861 |
| [isolation of mycobacterium tuberculosis and their l forms from patients with osteoarticular tuberculosis]. | | 1986 | 3086862 |
| [experience in treating tuberculosis patients in the ib outpatient registry group]. | | 1986 | 3086863 |
| [identification of mycobacteria using a thin-layer chromatographic method]. | | 1986 | 3086865 |
| [destructive tuberculosis of the respiratory organs in roentgen-positive and roentgen-negative persons]. | | 1986 | 3086987 |
| [tuberculosis of the prostate]. | | 1986 | 3087045 |
| a ten-year experience with fiberoptic bronchoscopy for mycobacterial isolation. impact of the bactec system. | from january 1974 to december 1983, positive mycobacterial isolates from all sources were reviewed to determine the impact of fiberoptic bronchoscopy (fb) on retrieval and identification of these organisms. there were 112 patients with positive cultures obtained during fb, 25 with mycobacterium tuberculosis and 87 with mycobacteria other than tuberculosis (mott). we reviewed the results of prebronchoscopy and postbronchoscopy sputum specimens, bronchial washings, brushings, and transbronchial bi ... | 1986 | 3087251 |
| evaluation of adenosine deaminase activity and antibody to mycobacterium tuberculosis antigen 5 in cerebrospinal fluid and the radioactive bromide partition test for the early diagnosis of tuberculosis meningitis. | a number of different biochemical and serological tests have been described recently for the early and accurate diagnosis of tuberculous meningitis. none of these tests has yet gained widespread acceptance in clinical medicine or in microbiology laboratories. to investigate this problem we evaluated adenosine deaminase activity (ada), an enzyme linked immunosorbent assay (elisa) that detects antibody to antigen 5 of mycobacterium tuberculosis, and the radioactive bromide partition test (bpt) in ... | 1986 | 3087296 |
| deoxyribonucleic acid replication time in mycobacterium tuberculosis h37 rv. | the dna increment method, designed for measuring the increment in the amount of dna after inhibition of initiation of fresh rounds of replication initiation was employed to measure the rate of deoxyribonucleic acid (dna) chain growth in mycobacterium tuberculosis h37rv growing in youman and karlson's medium at 37 degrees c with a generation time of 24 h and also in relatively fast growing species like mycobacterium smegmatis and escherichia coli. from the results obtained, the time required for ... | 1986 | 3087325 |
| pathologic quiz case 2. scrofula (mycobacterial lymphadenitis). | | 1986 | 3087381 |
| radiometric diagnosis of mycobacteria. | the aim of this study was to determine the impact of recently developed rapid radiometric techniques on the clinical diagnostic operations of a reference laboratory for mycobacteria. selective inhibition by rho-nitro-alpha-acetylamino-beta-hydroxypropiophenone was used to rapidly screen submitted cultures for identification of mycobacterial species other than mycobacterium tuberculosis. the radiometric drug susceptibility test was applied only to those cultures presumptively identified as belong ... | 1986 | 3087747 |
| identification of mycobacterium tuberculosis antigens in seibert fractions by immunoblotting. | seibert fractions prepared from mycobacterium tuberculosis culture filtrates were evaluated by immunoblotting with a serum pool from patients with active pulmonary tuberculosis. antibody activity was observed primarily with antigens in the polysaccharide ii and a protein fractions; these fractions were further evaluated by immunoblotting with sera from individual patients with tuberculosis, from individuals without tuberculosis and positive for the purified protein derivative antigen skin test, ... | 1986 | 3088029 |
| detection of mycobacterial antigens in leprosy serum immune complex. | the antigens from immune complexes of sera from patients with mycobacterial diseases were released by sodium dodecyl sulfate. the antigenic activity of the released proteins was tested by agar gel diffusion and immunoelectrophoresis. this simple method provided direct evidence for the presence of mycobacterial antigens in the immune complexes of sera from patients with leprosy and tuberculosis. | 1986 | 3088033 |
| mycobacterium tuberculosis bacteremia in the acquired immunodeficiency syndrome. | | 1986 | 3088290 |
| tuberculosis of the middle ear: review of the literature with an instructive case report. | tuberculosis of the middle ear is currently a rare disease in developed countries but one that still occurs and typically causes significant morbidity manifested by profound and permanent hearing loss. diagnosis is difficult since most physicians are unfamiliar with the typical presenting features; in addition, special cultural and pathologic studies are required for diagnosis. a case report and review of the literature are presented to delineate typical clinical, epidemiologic, and laboratory f ... | 1986 | 3088701 |
| [characteristics of bacteria shedding among patients with destructive pulmonary tuberculosis]. | | 1986 | 3088838 |
| tuberculosis in korea. the relationship between prior therapy and drug resistance. | a review of 121 culture-positive cases of pulmonary tuberculosis from 1979 to 1984, including both korean and american patients, at the major us military hospital in korea indicated that most antituberculosis drug resistance occurred in patients with a history of previous antituberculosis therapy. the 98 patients without previous therapy who were not household contacts of a known resistant case had low rates of drug resistance (7 percent to isoniazid, 5 percent to streptomycin, 2 percent to p-am ... | 1986 | 3089700 |
| [active pulmonary tuberculosis in lung cancer]. | | 1986 | 3089885 |
| melittin-stimulated antimycobacterial activity of the membrane fraction isolated from phagocytes of guinea pigs. | virulent tubercle bacilli were incubated in kirchner semi-solid agar medium with the membrane fraction prepared from guinea-pig peritoneal exudate cells. a marked inhibition of mycobacterial growth was observed in the presence of added melittin which is known as an activator of membrane phospholipase a. the bactericidal activity in a buffer environment was also demonstrated by the fraction alone and that stimulated by melittin. | 1986 | 3090321 |
| bactericidal activity of the membrane fraction isolated from phagocytes of mice and its stimulation by melittin. | the membrane fraction prepared from mouse peritoneal exudate cells was incubated with mycobacteria, staphylococci, or e. coli in acetate buffer of ph 5.6 to follow the fate of viable bacilli. the membrane fraction exhibited bactericidal effect on mycobacteria and staphylococci, but not on e. coli. the activity to kill mycobacteria, as well as the endogenous phospholipase a2 activity, of the membrane fraction was markedly enhanced by melittin, a basic peptide from bee venom, and inhibited by indo ... | 1986 | 3090322 |
| importance of lyt 1+ t-cells in the antitumor activity of an immunomodulator, ssm, extracted from human-type tubercle bacilli. | an arabinomannan lipid extracted from mycobacterium tuberculosis strain aoyama b (ssm) is an immunopotentiating agent with interferon-inducing and antitumor activities. in the present study, the possible role(s) of various immunocompetent cells on the antitumor effect of ssm was investigated in mice bearing syngeneic (rl male 1 leukemia) and allogeneic (ehrlich carcinoma) ascites tumors. when thy 1+ t-cells were depleted from tumor-bearing mice by the administration of monoclonal anti-thy 1.2 an ... | 1986 | 3090339 |
| [comparative studies on in vitro activities of important antituberculous drugs]. | | 1986 | 3090342 |
| [primary drug resistance to antitubercular drugs in patients with pulmonary tuberculosis in the years 1978-1982]. | | 1986 | 3090521 |
| antibody-based enzyme-linked immunosorbent assay for determination of immune complexes in clinical tuberculosis. | immune complexes were isolated from sera of tuberculosis patients by precipitation with 2.5% polyethylene glycol. the precipitates were characterized by quantitative determination of different immunoglobulin classes by single radioimmunodiffusion, sodium dodecyl sulfate polyacrylamide gel electrophoresis for presence of serum components, ouchterlony's double diffusion method for detection of complement components c3 and c4, and immuno-dot assay for detection of mycobacterium tuberculosis antigen ... | 1986 | 3090914 |
| supplement on future research in tuberculosis. prospects and priorities for elimination. endorsement of the american thoracic society board of directors in march 1986. | | 1986 | 3090919 |
| recombinant interferon-gamma and chemotherapy with isoniazid and rifampicin in experimental murine tuberculosis. | viable bacterial counts in the lungs and spleens of mice infected intravenously with mycobacterium tuberculosis, strain h37rv were reduced by intravenous recombinant murine interferon-gamma (ifn-gamma) 1000-5000 u, but not by 200 u. reduction in counts was greatest when ifn-gamma was given 1 day before infection and was not increased by additional doses in the preceding 2 days. the effect was complete in 1 day and was not increased by successive doses during the next week. giving ifn-gamma in mu ... | 1986 | 3091058 |
| humoral immune response in tuberculosis: initial characterization by immunoprecipitation of 125iodine labelled antigens and sodium dodecyl sulfate polyacrylamide gel electrophoresis. | 125iodine-labelled mycobacterium tuberculosis antigens were immunoprecipitated with tuberculosis patients' sera and analysed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. a group of four polypeptide antigens of 55, 38, 28 and 18 kd were thus identified. the 38 and 28 kd polypeptides were the major antigens. antibody response differed from one patient to another, both with respect to the number and quantity of antigens precipitated. untreated patients and those undergoing treatmen ... | 1986 | 3091299 |
| [immunologic diagnosis of tuberculous meningitis]. | | 1986 | 3091333 |