| a new species of the genus chauhanellus bychowsky et nagibina, 1969 (monogenea) from the gills of a sea fish tachysurus dussumieri (val.) from andhra pradesh, india. | chauhanellus chauhani sp. n., collected from the gills of a sea cat-fish, tachysurus dussumieri (valenciennes) from the bay of bengal, off the coast of kakinada, andhra pradesh, india is described and illustrated. it differs from the related species of the genus--c. oculatus, c. flexiosus, c. australis, c. nagibinae, and c. pedunculatus--in the shape and articulation of accessory piece with cirrus and in having a crescentic posteromedian process on its dorsal bar. it has five transverse hard str ... | 1990 | 2227713 |
| [glass ionomer cements as orthodontic bracket adhesives. an in vitro study with 4 glass ionomer cements (gic) and 2 conventional orthodontic bracket adhesives as the comparative group]. | 240 orthodontic brackets were bonded to buccal surfaces of bovine teeth. as bonding material we used four glass ionomer cements without etching and two normal orthodontic bracket bonding materials with etching of enamel. brackets of group 1 were tested with a material testing machine for shear and tensile strength after ten minutes. brackets of group 2, 3 and 4 were tested after one day, 28 days and 98 days, respectively. in the average the bonding strength of glass ionomer cements was 50% less ... | 1990 | 2227735 |
| [glass ionomer cements as orthodontic bracket adhesives. an in vitro study with 4 glass ionomer cements (gic) and 2 conventional orthodontic bracket adhesives as the comparative group]. | 240 orthodontic brackets were bonded to buccal surfaces of bovine teeth. as bonding material we used four glass ionomer cements without etching and two normal orthodontic bracket bonding materials with etching of enamel. brackets of group 1 were tested with a material testing machine for shear and tensile strength after ten minutes. brackets of group 2, 3 and 4 were tested after one day, 28 days and 98 days, respectively. in the average the bonding strength of glass ionomer cements was 50% less ... | 1990 | 2227735 |
| phage typing of vibrio cholerae 01 biotype elt or strains. | data accumulated over the past 20 yr (1969-88) on the phage typing of v. cholerae 01 biotype elt or strains received at the vibrio phage reference laboratory, were retrospectively analysed to ascertain the frequency of occurrence of the different phage types of basu and mukerjee typing scheme. the analysis revealed that phage types 2 and 4 were the most common types not only at the time when the basu and mukerjee typing scheme (of 1968) was introduced but also at present. the existing phage typi ... | 1990 | 2228054 |
| high-frequency spontaneous mutation of classical vibrio cholerae to a nonmotile phenotype. | the species vibrio cholerae contains within it two biotypes, classical and el tor, both of which are motile. phenotypic expression of motility was unaffected by type of growth medium, salt concentration, ph, or temperature of incubation. however, seven strains of classical v. cholerae produced spontaneous nonmotile mutants at an unusually high frequency (ca. 10(-4)), while no mutants were detected for all three el tor strains examined. no revertants of these nonmotile mutants were detected. four ... | 1990 | 2228234 |
| purification and characterization of a novel hemagglutinin from vibrio cholerae. | a lectin with strong hemagglutinating activity toward erythrocytes of several animal species was isolated from an 18-h culture supernatant of a diarrheagenic strain, v2, of non-o1 vibrio cholerae. the hemagglutinin (ha) was purified free of lipopolysaccharide by salt fractionation followed by gel filtration, hydrophobic interaction chromatography, and, finally, gel filtration in the presence of urea and deoxycholate. the purification procedure resulted in an ha preparation with 80-fold enhanceme ... | 1990 | 2228240 |
| recombinant fusion protein for simple detection of escherichia coli heat-stable enterotoxin by gm1 enzyme-linked immunosorbent assay. | a recombinant gene fusion protein composed of an escherichia coli heat-stable enterotoxin (sta) peptide epitope fused to the amino end of the cholera toxin b subunit was used to detect sta produced by clinical isolates of enterotoxigenic e. coli (sta-etec) by a single monoclonal antibody-based inhibition gm1 enzyme-linked immunosorbent assay. in this test, 100% sensitivity and 100% specificity were observed for use of the recombinant protein in either its purified form or as crude vibrio cholera ... | 1990 | 2229340 |
| cholera outbreak in delhi--1988. | delhi experienced an outbreak of cholera during july-august 1988 which affected residents from all walks of life. a total of 1824 laboratory confirmed cholera cases were detected in two months period at i.d. hospital, delhi alone. the number of cholera cases in july-august 1988 was 5-10 times that of the same period during the previous years in the capital. the outbreak was caused by vibrio cholerae ogawa biotype eltor. majority of the laboratory confirmed cases (about 74 per cent) were seen in ... | 1990 | 2230018 |
| plasmids and factors associated with virulence in environmental isolates of vibrio cholerae non-o1 in bangladesh. | plasmid profiles and factors associated with toxigenicity in 51 strains of vibrio cholerae non-o1 isolated from water samples collected in bangladesh were analysed. eleven (21.5%) strains were found to harbour at least one plasmid of 1.7-115 mda; seven of these strains shared a 115-mda plasmid. six of 13 strains tested gave positive cytotoxic and enterotoxic responses. however, two non-cytotoxic strains were enterotoxigenic. only three of the six cytotoxic and enterotoxic strains caused haemaggl ... | 1990 | 2231676 |
| confirmation by peptide sequence and co-expression on various cell types of the identity of cd44 and p85 glycoprotein. | the p85 glycoprotein expressed on a variety of human cell types including astrocytes and lymphocytes has not been associated with the cd44 cluster. the recent demonstration that hermes, a glycoprotein implicated in the adhesion of lymphocytes to endothelium, belongs to the cd44 cluster raises interesting questions concerning the role of this molecule on astrocytes and on non-lymphoid cells. to obtain confirmation of the identity of p85 glycoprotein and cd44, p85 glycoprotein was purified from b- ... | 1990 | 2233756 |
| [immunochemical characteristics of vibrio neuraminidases isolated from aerated culture filtrates]. | immunochemical analysis of neuraminidases, isolated from choleraic and non-choleraic vibrios, was carried out. antineuraminidase antiserum was used in procedures of immunoelectrophoresis, diffusion precipitation in agar gel and disc immunoelectrophoresis in polyacrylamide gel. antigen composition of neuraminidases was shown to be similar both in choleraic and non-choleraic vibrios. | 1990 | 2238539 |
| [effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog]. | a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ... | 1990 | 2238764 |
| [the antimicrobial action of the xanthine oxidase-xanthine system on the causative agent of cholera]. | as revealed in experiments on v. cholerae, the enzymatic link xanthine oxidase-xanthine produces a vibriostatic effect at the concentration of xanthine oxidase equal to 0.0125 g/l and a vibriocidal effect at the concentration of xanthine oxidase equal to 0.025 g/l in a medium with ph 7.5-7.6. in the presence of protein the antivibrionic activity of the xanthine oxidase link is decreased. the introduction of bivalent iron into the enzymatic link xanthine oxidase-xanthine enhances its vibriocidal ... | 1990 | 2239000 |
| [the immune status of children in foci of measles infection studied by an immunoenzyme method]. | children immunized with live measles vaccine in the foci of measles infection varying in intensity (1-9 cases per focus) have been subjected by two methods: the hemagglutination inhibition (hai) test and the enzyme immunoassay (eia). as shown in this study, in most cases (98% of all blood serum samples) the correlation between the results of the hai test and eia is not high (r = 0.5), which is linked with the detection of a wider spectrum of antibodies in eia. the percentage of seronegative chil ... | 1990 | 2239001 |
| [the immune status of children in foci of measles infection studied by an immunoenzyme method]. | children immunized with live measles vaccine in the foci of measles infection varying in intensity (1-9 cases per focus) have been subjected by two methods: the hemagglutination inhibition (hai) test and the enzyme immunoassay (eia). as shown in this study, in most cases (98% of all blood serum samples) the correlation between the results of the hai test and eia is not high (r = 0.5), which is linked with the detection of a wider spectrum of antibodies in eia. the percentage of seronegative chil ... | 1990 | 2239001 |
| glycerol-blanked triglyceride assays with laboratory animals. | | 1990 | 2242600 |
| chlamydial igg and iga in serum and follicular fluid among patients undergoing in vitro fertilisation. | the point prevalence of igg and iga antibodies to chlamydia was analyzed in serum and follicular fluid in 63 patients undergoing in vitro fertilisation (ivf) in comparison to sera of 298 healthy women by the single serovar (l2) inclusion immunoperoxidase assay (ipa). the presence of specific igg and iga to chlamydia in follicular fluid was demonstrated. no statistical association was found between the presence of specific chlamydia igg and iga in serum and follicular fluid to oocyte fertilizatio ... | 1990 | 2242796 |
| a cholera outbreak associated with eating uncooked pork in thailand. | in a village near chiangmai, thailand, during october 1987, there was an outbreak of cholera following a funeral in which 264 attendants were served food. the present article is a report of an epidemiological study performed to identify the source of infection and the mode of its transmission. all the attendants were screened for infection by bacteriological examination of their rectal swabs and were kept under diarrhoeal surveillance. of them, 20 patients and 40 matched controls were interviewe ... | 1990 | 2243182 |
| segmental vascular resistance in postobstructive pulmonary vasculopathy. | chronic unilateral pulmonary arterial ligation has been touted as a model of arteriopathy resulting in a tremendous increase in anastomotic bronchial flow (qbr) via collaterals. to investigate its effects on the pulmonary vasculature, we ligated the left main pulmonary artery of seven dogs and 120 days later pump perfused their left lower lobes (lll) via a cannula in the pulmonary artery at pulmonary arterial flow (qpa) of 250 ml/min. we measured qbr (330 ml/min) and compared lll with control co ... | 1990 | 2246149 |
| 3,4-dihydroxyphenylacetic acid (dopac) as an index of noradrenaline turnover: effects of hydergine and vincamine. | among the drugs commonly used in the treatment of memory disorders of the elderly, vincamine and hydergine have been shown to moderately increase the firing rate of noradrenergic locus coeruleus (lc) neurons. since changes in electrical activity of noradrenergic neurons are generally reflected in corresponding alterations of the turnover of this transmitter, the effects of these drugs on the accumulation of 3,4-dihydroxyphenylacetic acid (dopac) and dopamine (da) in the presence and absence of t ... | 1990 | 2248732 |
| 3,4-dihydroxyphenylacetic acid (dopac) as an index of noradrenaline turnover: effects of hydergine and vincamine. | among the drugs commonly used in the treatment of memory disorders of the elderly, vincamine and hydergine have been shown to moderately increase the firing rate of noradrenergic locus coeruleus (lc) neurons. since changes in electrical activity of noradrenergic neurons are generally reflected in corresponding alterations of the turnover of this transmitter, the effects of these drugs on the accumulation of 3,4-dihydroxyphenylacetic acid (dopac) and dopamine (da) in the presence and absence of t ... | 1990 | 2248732 |
| field trial of oral cholera vaccines in bangladesh: evaluation of anti-bacterial and anti-toxic breast-milk immunity in response to ingestion of the vaccines. | in a field trial conducted in bangladesh, ingestion of either b subunit-killed whole cell (bs-wc) or killed whole cell (wc) oral cholera vaccines by mothers was associated with a 47% reduction of the risk of cholera in their non-vaccinated children aged under 36 months. because vaccine-induced breast-milk immunity seemed a possible explanation for these findings, we evaluated anti-lipopolysaccharide (lps) and anti-cholera toxin (ct) iga antibody responses in breast milk collected during the tria ... | 1990 | 2251873 |
| molecular cloning and nucleotide sequence analysis of cholera toxin genes of the ctxa- vibrio cholerae strain texas star-sr. | the ctx operons from the vibrio cholerae el tor strain 3083 and its ctxa- derivative texas star-sr were cloned, and their nucleotide sequences were compared. a single missense mutation in the texas star-sr ctxa cistron which results in the substitution of threonine for alanine at position 191 of the 258-amino-acid ctxa precursor was identified. immunoblot analysis detected the mutant ctxa intracellularly early in the culture cycle but not extracellularly at any growth stage. | 1990 | 2254034 |
| purification and characterization of a protease produced by vibrio mimicus. | a protease produced by vibrio mimicus was purified to apparent homogeneity by ammonium sulfate fractionation and successive column chromatography on sephacryl s-100 and mono q monobeads. the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (page) of the final preparation of the enzyme revealed the homogeneity of the purified enzyme. conventional page showed that the purified protease migrated as a single band with protease activity. the molecular weight of the protease was estimated to ... | 1990 | 2254038 |
| neuroendocrine measurements in steers grazed on endophyte-infected fescue. | dopamine (da), serotonin (5ht) and selected precursors and metabolites were measured in the anterior pituitary gland, hypothalamus and pineal gland, along with serum prolactin (prl) and average daily gains (adg), in steers (n = 6/group) grazing endophyte (acremonium coenophialum)-infected and noninfected fescue (festuca arundinacea schreb). paddocks (two/treatment) were designated 100f and 0f (100 and 0% infection, respectively). after 6 wk, three animals from one of the 100f paddocks were excha ... | 1990 | 2254203 |
| in vitro and in vivo anticancer activity of mitozolomide and sparsomycin in human tumor xenografts, murine tumors and human bone marrow. | the colony formation in agar of human tumor xenografts, of murine tumors and of human bone marrow was used as a test system to determine the in vitro activity of the two novel cytostatic agents, mitozolamide and sparsomycin. mitozolomide was additionally studied in vivo in nine human tumor xenografts. the comparison of in vitro/in vivo activity allows an assessment of the relevant in vitro dose based on in vivo pharmacological behavior of a compound. both compounds showed clear dose/response eff ... | 1990 | 2254373 |
| mhc-restricted antibody responses to trichuris muris excretory/secretory (e/s) antigen. | two panels of h-2 recombinant mice were used in a detailed serological study to analyse the role of h-2-linked genes in the control of the antibody response to excretory/secretory (e/s) antigens of trichuris muris. an apparent h-2q (i-aq) restriction on the early development of high levels of igg1 antibody to e/s antigen was revealed by elisa. no such restriction was demonstrated for the specific igg2a response patterns. recognition of two high molecular weight antigens (90-95 kda, 105-110 kda) ... | 1990 | 2255562 |
| tgf-alpha and tgf-beta expression during sodium-n-butyrate-induced differentiation of human keratinocytes: evidence for subpopulation-specific up-regulation of tgf-beta mrna in suprabasal cells. | sodium-n-butyrate (nab) induces terminal differentiation and cornified envelope formation in cultured human keratinocytes. in the present study we explored the question of whether nab-induced maturation could be mediated through changes in tgf-alpha and tgf-beta expression in normal keratinocytes. nab induced a four-fold increase in tgf-beta mrna transcript levels. this increase in tgf-beta mrna abundance occurred only within the nonbasal keratinocyte subpopulation which maximally responds to na ... | 1990 | 2257881 |
| tgf-alpha and tgf-beta expression during sodium-n-butyrate-induced differentiation of human keratinocytes: evidence for subpopulation-specific up-regulation of tgf-beta mrna in suprabasal cells. | sodium-n-butyrate (nab) induces terminal differentiation and cornified envelope formation in cultured human keratinocytes. in the present study we explored the question of whether nab-induced maturation could be mediated through changes in tgf-alpha and tgf-beta expression in normal keratinocytes. nab induced a four-fold increase in tgf-beta mrna transcript levels. this increase in tgf-beta mrna abundance occurred only within the nonbasal keratinocyte subpopulation which maximally responds to na ... | 1990 | 2257881 |
| a reappraisal of the role of methimazole and other factors on the efficacy and outcome of radioiodine therapy of graves' hyperthyroidism. | the outcome of radioiodine therapy of graves' hyperthyroidism was retrospectively evaluated in 274 consecutive patients treated from 1975 to 1984. at 1-yr follow-up, permanent hypothyroidism occurred in 36.9% of patients and the cumulative incidence of hypothyroidism progressively increased up to 79.3% after 7-10 yr. at the end of the follow-up period, 148 patients (54%) were hypothyroid, 115 (42%) euthyroid and 11 (4%) still hyperthyroid. the prevalence of hypothyroidism was significantly highe ... | 1990 | 2258580 |
| a reappraisal of the role of methimazole and other factors on the efficacy and outcome of radioiodine therapy of graves' hyperthyroidism. | the outcome of radioiodine therapy of graves' hyperthyroidism was retrospectively evaluated in 274 consecutive patients treated from 1975 to 1984. at 1-yr follow-up, permanent hypothyroidism occurred in 36.9% of patients and the cumulative incidence of hypothyroidism progressively increased up to 79.3% after 7-10 yr. at the end of the follow-up period, 148 patients (54%) were hypothyroid, 115 (42%) euthyroid and 11 (4%) still hyperthyroid. the prevalence of hypothyroidism was significantly highe ... | 1990 | 2258580 |
| staphylococcus aureus infection of human endothelial cells potentiates fc receptor expression. | vasculitis, a recognized complication of staphylococcal-endovascular infections, may result in part, from the expression of fcr by staphylococcus aureus-infected endothelial cells. fcr were measured using [51]cr labeled srbc preincubated with rabbit anti-srbc igg. fcr were not detected on uninfected endothelial cells, but were demonstrated on s. aureus infected cells using igg, but not igm labeled srbc. fcr expression was dependent on the initial bacterial density (greater than or equal to 8 x 1 ... | 1990 | 2258617 |
| [detection of toxigenic vibrio cholerae o1 using polymerase chain reaction for amplifying the cholera enterotoxin gene]. | a rapid and simple procedure using polymerase chain reaction (pcr) was developed for the detection of cholera enterotoxin (ct) producing character. this method is based on amplifying a 380 base pair (bp) segment of the ct gene (ctx) which controls the production of ct. two single-stranded oligonucleotides, synthetized to be complementary to the known nucleotide sequences of genes encoding the a-subunit of ctx, were used as extension primers. the oligonucleotide sequences are 5'tcaaactatattgtctgg ... | 1990 | 2258648 |
| [development and testing of cholera enterotoxin gene probe for detection of toxigenic vibrio cholerae o1]. | a dna probe was developed for the genetic detection from cholera enterotoxin (ct) producing vibrio cholerae o1 and other organisms. the structural genes of ct (ctx) were cloned from chromosomal dna of ct producing v. cholerae o1 569b. we subcloned a 552-base-pair fragment encoding a part of ct a-subunit for use of the ct-probe, and made the recombinant plasmid called pskm24 which has eight copies of the ct-probe. the 32p-labeled ct-probe detected ctx in 72 isolates such as v. cholerae o1, v. cho ... | 1990 | 2258649 |
| [clinical, bacteriological, and immunological examination and treatment of two papillon-lefevre syndrome patients]. | papillon-lefèvre syndrome (pls) is a rare disease accompanied by palmo-plantar hyperkeratosis and rapidly progressive periodontal breakdown of deciduous and permanent dentition. two unrelated female pls patients, four and seven years old, with severe periodontal destruction were examined. antibody titers against seven strains by the enzyme-linked immunosorbent assay (elisa), microbial cultures from deep periodontal pockets and mouth rinse samples and immunoblotting analysis were performed. titer ... | 1990 | 2258685 |
| [clinical, bacteriological, and immunological examination and treatment of two papillon-lefevre syndrome patients]. | papillon-lefèvre syndrome (pls) is a rare disease accompanied by palmo-plantar hyperkeratosis and rapidly progressive periodontal breakdown of deciduous and permanent dentition. two unrelated female pls patients, four and seven years old, with severe periodontal destruction were examined. antibody titers against seven strains by the enzyme-linked immunosorbent assay (elisa), microbial cultures from deep periodontal pockets and mouth rinse samples and immunoblotting analysis were performed. titer ... | 1990 | 2258685 |
| stimulus train-induced bursting in hippocampal slices: difference between ca1 and ca2/3 subfields. | we studied the relationship between ca2/3 and ca1 subfields in the generation of stimulus train-induced bursting using in vitro hippocampal slices. repeated stimulation to the stratum radiatum induced three types of epileptiform activities: afterdischarge bursts, spontaneous bursts and single stimulus-triggered bursts. in the intact slices, spontaneous bursts in ca3 preceded those in ca1, whereas afterdischarge bursts in ca3 followed those in ca1 when it was possible to distinguish differences i ... | 1990 | 2259016 |
| survival of toxigenic vibrio cholerae o1 with a common duckweed, lemna minor, in artificial aquatic ecosystems. | cholera epidemics occur twice a year in bangladesh. during epidemics, vibrio cholerae o1 are isolated from patients, as well as from the surface water, but the bacteria disappear during inter-epidemic periods. their reservoirs or sites of survival and multiplication during inter-epidemic period are still unknown. the present survival study in the laboratory explored the role of an aquatic plant, lemna minor (duckweed), as a possible reservoir. l. minor was added to sea-salt solution at ph 8.5, c ... | 1990 | 2260179 |
| polyamine metabolism and uptake during phaseolus vulgaris lectin, pha-induced growth of rat small intestine. | kidney bean lectin, pha, stimulated the hyperplastic and hypertrophic growth of rat small intestine. this growth was preceded by a rapid accumulation of polyamines in the small intestine. however, since the lectin had little effect on in situ polyamine biosynthesis, most of the polyamines must have been of extracellular origin. to investigate the source of polyamines, both the luminal uptake and basolateral transport of polyamines by the rat small intestine were measured in vivo. luminal polyami ... | 1990 | 2262068 |
| two autosomal trisomies in the horse: 64,xx,-26,+t(26q26q) and 65,xx,+30. | the phenotypic effects in a yearling arab filly of a newly described equine autosomal trisomy syndrome for chromosome 30 (65,xx,+30) consisted of small size and severe angular deviation of front legs accompanied by mild polydactyly, but no mental dullness. this case was associated with advanced maternal age. additional banding studies of a second trisomy case confirmed the assignment to chromosome 26 (64,xx,-26,+t(26q26q)) and evidence of her fertility was presented. | 1990 | 2262139 |
| [microbiological assay method of bmy-28100 and some other cephem antibiotic in serum and tissues. development of new sample preparation with evan's blue, a high albumin binding dye]. | with a new simple sample pretreatment, microbiological assay methods for quantitative determination of bmy-28100 and some other cephem antibiotics in serum and tissues have been developed. presented sample pretreatment was founded on the extraction with evans' blue, a high serum protein binding dye, and the ultrafiltration using cellulose membrane (millipore; mw cutoff is 10,000) to which evans' blue was specifically absorbed. regardless of sample media, antibiotic contents in filtrates were det ... | 1990 | 2262961 |
| a new sensitive fluorometric assay for the metabolism of (--)-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene by human hair follicles. | a new sensitive fluorometric assay was established to measure the stereospecific formation of benzo[alpha]pyrene tetrols formed after cytochrome p450-dependent metabolism of (--)-7,8-dihydroxy-7,8- dihydrobenzo[a]pyrene by human hair follicles. this simple assay requires three human hair follicles and a low (0.5-2.0 microm) substrate concentration and has a limit of detection of approximately 0.3 fmol of tetrols. freshly isolated human hair follicles from 20 adult volunteers (10 non-smokers and ... | 1990 | 2265467 |
| kinetic study of esterification by immobilized lipase in n-hexane. | the kinetic of the esterification of oleic acid by ethanol catalyzed by immobilized lipase of mucor miehei in n-hexane as a solvent has been completely studied. the kinetics of the reaction are suggested to agree with a ping-pong bi bi mechanism in which only inhibition by excess of ethanol has been identified. values of all apparent kinetic parameters were computed. no evidence of any significant external diffusional limitation which could account for these values has been detected. optimizatio ... | 1990 | 2265698 |
| the molecular mode of brain mrna processing damage followed by the suppression of post-transcriptional poly(a) synthesis with cordycepin. | complete suppression of polyadenylation of nuclear precursors of rat brain mrna by cordycepin leads to degradation of some translatable sequences of both poly(a)(+)- and poly(a)- pre-mrna localized in 80s hnrnp-particles. this fact has been established by comparative analysis of the data of two-dimensional gel-electrophoretic mapping of translation products synthesized in reticulocytic cell-free system using the exogenous purified templates of rapidly labelling translatable 9-17s hn rna (pre-mrn ... | 1990 | 2265908 |
| selective inhibition of growing pigment epithelial cells by a receptor-directed immunotoxin. | an immunotoxin conjugate of a murine monoclonal antibody against human transferrin receptors and the a chain of ricin was examined for its potential to inhibit selectively the growth of retinal pigment epithelial (rpe) cells which grow in an uncontrolled manner in proliferative vitreoretinopathy. the probable efficacy of such an agent in vivo stems from the observation that actively proliferating cells possess many more transferrin receptors than normal quiescent cells. the authors showed in vit ... | 1990 | 2265991 |
| nonagglutinable vibrio cholerae septicaemia. | | 1990 | 2266091 |
| increased toxin production by vibrio cholerae o1 during survival with a green alga, rhizoclonium fontanum, in an artificial aquatic environment. | in the aquatic environment, the physiological state of vibrio cholerae can be affected by various environmental conditions (e.g., sunlight, ph, temperature, competition with other bacteria for nutrients, etc.). the effect of these factors on the toxigenicity of v. cholerae was investigated. toxin production by 5 toxigenic strains of v. cholerae incubated in laboratory microcosms containing rhizoclonium fontanum was tested at different time intervals. the microcosms were exposed to sunlight, and ... | 1990 | 2266879 |
| an animal model of motor neuron disease in guinea pigs. | | 1990 | 2267309 |
| spontaneous primary astrocytoma in the spinal cord of a mouse and a rat. | a spontaneous astrocytoma was observed as an incidental finding in the thoracic spinal cord of a control charles rivers cd-1 female mouse from a 2 yr oncogenicity study. an astrocytoma was also observed in the lumbar spinal cord of a charles rivers sprague-dawley control, female rat from a 2 yr oncogenicity study. the mouse was clinically normal and was sacrificed at the end of the 2 yr study. the rat was killed at 512 days of age because of posterior paralysis. | 1990 | 2267503 |
| pneumocystis carinii pneumonia in danish piglets. | | 1990 | 2267715 |
| fate of injected glucagon taken up by rat liver in vivo. degradation of internalized ligand in the endosomal compartment. | the uptake and processing of glucagon into liver endosomes were studied in vivo by subcellular fractionation. after injection of [[125i]iodo-tyr10]glucagon and [[125i]iodo-tyr13]glucagon to rats, the uptake of radioactivity into the liver was maximum at 2 min (6% of the dose/g of tissue). on differential centrifugation, the radioactivity in the homogenate was recovered mainly in the nuclear (n), microsomal (p) and supernatant (s) fractions, with maxima at 5, 10 and 40 min, respectively; recovery ... | 1990 | 2268296 |
| human brain n-chimaerin cdna encodes a novel phorbol ester receptor. | a human brain-specific cdna encoding n-chimaerin, a protein of predicted molecular mass 34 kda, has sequence identity with two different proteins: protein kinase c (pkc) at the n-terminus and bcr protein [product of the breakpoint-cluster-region (bcr) gene, involved in philadelphia chromosome translocation] at the c-terminus [hall, monfries, smith, lim, kozma, ahmed, vannaisungham, leung & lim (1990) j. mol. biol. 211, 11-16]. the sequence identity of n-chimaerin with pkc includes the cysteine-r ... | 1990 | 2268301 |
| survey of enteropathogenic agents in children with and without diarrhoea in ghana. | a survey was carried out over 1 year in a rural area of ghana on the isolation, detection and/or identification of enteric pathogens from children under 5 years of age with and without diarrhoea. the isolation and detection rate of shigella flexneri, shigella dysenteriae, giardia lamblia and rotavirus were higher in children with diarrhoea than in controls. yersinia enterocolitica, vibrio cholerae and vibrio parahaemolyticus were not isolated during the period of this survey. the incidence of ot ... | 1990 | 2270006 |
| [from the parenchyma to the mucosa biopsy: significance and importance of local assays of antibiotics. a critical study]. | the authors discussed the relevance of antibiotic concentrations as measured in tissues and fluids of the respiratory tract. they analysed theoretical factors which influence tissue distribution of antibiotics as well as the results of studies carried out in animal and in human tissues. various factors may interfere with the results of antibiotic assays: but the data published by various groups were usually convergent: this confirms the significance of "tissue pharmacokinetics". new protocols ba ... | 1990 | 2270342 |
| marrying age and services. | | 1990 | 2271133 |
| acute pulmonary oedema on the ruwenzori mountain range. | a 40 year old man had an episode of severe pulmonary oedema at 4000-5000 m during the ascent of the margherita peak (5109 m) of mount stanley on the ruwenzori. he had taken acetazolamide and high dose dexamethasone to treat symptoms of acute mountain sickness. six years before he had been studied by right heart catheterisation as a healthy volunteer during hypoxic breathing at sea level. his pulmonary vascular reactivity had been within the normal range for 32 healthy subjects. this man had high ... | 1990 | 2271350 |
| phosphorescence and optically detected magnetic resonance studies of echinomycin-dna complexes. | echinomycin complexes with polymeric dnas and model duplex oligonucleotides have been studied by low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (odmr) spectroscopy, with the quinoxaline chromophores of the drug used as intrinsic probes. although not optically resolved, plots of odmr transition frequencies versus monitored wavelength revealed heterogeneity in the phosphorescence emission of echinomycin, which was ascribed to the presence of two distinct ... | 1990 | 2271577 |
| synthetic peptide model of an essential region of an aminoacyl-trna synthetase. | a 40 amino acid sequence of the unsolved structure of escherichia coli alanine-trna synthetase is essential for trna binding and encodes an immunological determinant that cross-reacts with antibodies raised against a eukaryote (insect bombyx mori) alanine enzyme. the secondary structure of this sequence is predicted to be an amphiphilic alpha-helix that includes one aspartyl and eight glutamyl side chain carboxyl groups. the antibody reactivity and the conformation of a synthetic peptide model o ... | 1990 | 2271589 |
| ribosomal protein l35: identification in spinach chloroplasts and isolation of a cdna clone encoding its cytoplasmic precursor. | we describe the isolation of spinach chloroplast ribosomal protein l35 and characterization of a cdna clone encoding its cytoplasmic precursor. this protein was only recently identified in ribosomes, but the sequences of four l35 genes have now been reported and confirm its presence in eubacteria, chloroplasts, and cyanelles. using n-terminal sequence data, oligonucleotides were designed and a cdna library was screened. the nucleotide sequence of the cdna clones shows that the spinach l35 protei ... | 1990 | 2271612 |
| heterogeneous initiation due to slippage at the bacteriophage 82 late gene promoter in vitro. | rnas synthesized in vitro by purified escherichia coli rna polymerase from a bacteriophage 82 promoter are heterogeneous at the 5' end. we show that this heterogeneity results from variable addition of extra adenine residues, allowed by slippage of the initial oligonucleotide pppaaa-oh against its dna template sequence ttt. slippage backward by one base allows another a to be added, giving pppaaaa-oh, and this cycle can continue more than 20 times before it is ended by incorporation of ump encod ... | 1990 | 2271677 |
| platinum compounds with estrogen receptor affinity. | | 1990 | 2272037 |
| [the structure-function relationships in alpha-interferons. a study of the antiviral activity of mutant forms of human alpha 2-interferon]. | | 1990 | 2272281 |
| in vitro diffusion of lidocaine across endotracheal tube cuffs. | smooth emergence from general endotracheal anesthesia is frequently complicated by coughing induced by stimulation from an endotracheal tube. lidocaine and other local anesthetics have been shown to anesthetize important rapidly adpating stretch receptors in the dog trachea. with the aim of providing a reservoir for continuous lidocaine release to adjacent tracheal tissue, we examined the ability of clinically used concentrations of lidocaine to diffuse across a commonly used endotracheal tube c ... | 1990 | 2275911 |
| in vitro diffusion of lidocaine across endotracheal tube cuffs. | smooth emergence from general endotracheal anesthesia is frequently complicated by coughing induced by stimulation from an endotracheal tube. lidocaine and other local anesthetics have been shown to anesthetize important rapidly adpating stretch receptors in the dog trachea. with the aim of providing a reservoir for continuous lidocaine release to adjacent tracheal tissue, we examined the ability of clinically used concentrations of lidocaine to diffuse across a commonly used endotracheal tube c ... | 1990 | 2275911 |
| partial characterization of a highly conserved aspartyl kinase (ak) in normal human liver. | subcellular fractions from human liver were assayed for aspartyl kinase (ak) activity measured by standard spectrophotometric methods. along the purification procedure three different fractions displayed the expected enzyme activity. interestingly, two of these fractions were specifically recognized by antibodies raised against e. coli aspartate kinases, suggesting a high degree of evolutionary conservation for these ubiquitous enzymes for prokaryotes. since their known function in bacteria is n ... | 1990 | 2279261 |
| toxoplasma antigens recognized by human immunoglobulin a antibodies. | the immunoglobulin a (iga) antibody response to antigens of toxoplasma gondii during the acute and chronic stages of t. gondii infection was studied by immunoblotting with a nonreduced antigen (nra) preparation. serum samples were from individuals with acute or chronic t. gondii infection, and sequential samples were from women who seroconverted during gestation or congenitally infected infants. iga antibodies to a variety of antigens were present in sera from each of the groups, irrespective of ... | 1990 | 2279993 |
| genetic transformation of vibrio parahaemolyticus, vibrio alginolyticus, and vibrio cholerae non o-1 with plasmid dna by electroporation. | an electroporation procedure for the plasmid-mediated transformation of the genus vibrio was performed, as part of an effort to develop recombinant dna techniques for genetic manipulation of the genus vibrio. vibrio parahaemolyticus, v. alginolyticus, and v. cholerae non o-1 (9 different strains) were transformed with 3 vector plasmids (pacyc184, phsg398, and pbr325). the efficiency of transformation was highly dependent on three parameters: the concentration of plasmid dna; the strength of the ... | 1990 | 2280728 |
| electron microscopic study of the polymyxin treated vibrio cholerae cells. | polymyxin b produces dose dependent changes in the surface topography of pathogenic vibrio cholerae cells. the susceptibilities of various vibrio strains to pb are also studied through analytical techniques. statistical analysis shows significant differences among the four vibrios with regard to their sensitivities to pb, the classical strains being the most sensitive. treating the classical strain with subinhibitory concentration of pb, we observed with both sem and tem that the normal smooth s ... | 1990 | 2282116 |
| clonal diversity of vibrio cholerae o1 evidenced by rrna gene restriction patterns. | the rrna gene restriction patterns of 89 vibrio cholerae o1 isolates from different geographic origins were studied. the probe was escherichia coli 16 + 23s rrna labelled with "ecl gene detection system". a total of 17 rrna gene restriction patterns were observed after bgli cleavage. four patterns (b1 to b4) were only given by biotype cholerae (14 strains studied). thirteen patterns (b5 to b17) were only given by biotype el tor (75 strains studied). there was no correlation between serotypes and ... | 1990 | 2284500 |
| non-o group 1 vibrio cholerae: a look at the epidemiology of an occasional pathogen. | non-o1 v. cholerae is a ubiquitous environmental isolate. it is a common contaminant of shellfish, and in the developing world, it is frequently isolated from food and water. asymptomatic carriage rates approaching 4 percent have been described among persons involved in high-risk activities, such as eating oysters in new orleans or going on pilgrimage to mecca. the actual occurrence of disease appears to be much less common. this is an "occupational" pathogen which may be responsible for outbrea ... | 1990 | 2286218 |
| antigenic and catalytic disparity in the distribution of cytochrome p-450-dependent 25-hydroxyvitamin d3-1 alpha- and 24-hydroxylases. | chick 25-hydroxyvitamin d3-1 alpha-hydroxylase, a cytochrome p-450 monooxygenase with a molecular weight of 57 kda, can be isolated as described by mandel et al. (1990 b). under normal physiological circumstances, it occurs exclusively in kidney mitochondria. an isozyme of the 1 alpha-hydroxylase, known as the 24-hydroxylase, which uses the same substrate to yield an isomeric product, is also a cytochrome p-450 monooxygenase, has a molecular weight of 55 kda, and like-wise occurs in kidney mitoc ... | 1990 | 2286531 |
| growth hormone receptors in hypothalamic and extra-hypothalamic tissues. | central gh receptors (ghr) have been identified in hypothalamic and extra-hypothalamic tissues of rabbit and chicken brains. plasma membranes of the rabbit brain demonstrated specific saturable high-affinity, low-capacity binding sites for 125i-labelled gh. rna extracted from hypothalamic and extra-hypothalamic tissues of rabbit and chicken brains contained mrna that hybridized with a cdna probe for the rabbit liver ghr. this transcript was of a similar size to the major ghr mrna moiety in rabbi ... | 1990 | 2288635 |
| neural transplantation in parkinson's disease: the swedish experience. | | 1990 | 2290977 |
| neural transplantation in parkinson's disease: the swedish experience. | | 1990 | 2290977 |
| development and regulation of growth and differentiated function in human and subhuman primate fetal gonads. | we have attempted to summarize the research on primate fetal gonadal development that has occurred over the past three decades. many similarities exist between fetal gonadal development in human and subhuman primates; therefore, comparisons and analogies between these species can be made. fetal gonadal development is a complex process dependent on timely maturation and differentiation of several cell types with different functions. adequate development is important for normal sexual development ... | 1990 | 2292242 |
| generation of affinity for antithrombin iii by supplemental sulfation of heparin species with low affinity for the protein. | the tributylammonium salt of a porcine heparin subfraction with low affinity for antithrombin iii (mr 7,500-18,000; anti-clotting activity, 7 usp units/mg), having degrees of sulfate substitution at d-glucosamine and l-iduronic acid residues of glcns 0.786, glcn6s 0.628, and idoa2s 0.682 mol, was reacted with 10 or 20 mol of pyridine-sulfur trioxide per mol equiv. of available hydroxyl groups in n,n-dimethylformamide at -10 degrees c for 1 h. both chemical and nmr spectroscopic analyses revealed ... | 1990 | 2292586 |
| chemical modification of pig liver initiation factor eif-2 with n-ethylmaleimide. amino acid sequences around the n-ethylmaleimide-reactive sulfhydryl groups and the effect of gdp on the modification. | the activity of eukaryotic initiation factor eif-2 as to the formation of the ternary complex, eif-2 gtp met-trna(f), is inhibited by n-ethylmaleimide. our preparation of pig liver eif-2 contained alpha and gamma subunits and was inhibited by more than 90% by n-ethylmaleimide. using our eif-2, we determined the sequences around the n-ethylmaleimide-reactive sulfhydryl groups, studied the effect of gdp on the sulfhydryl modification and that of nem on the [3h]gdp binding, and examined the protect ... | 1990 | 2292592 |
| a sensitive in vitro functional assay to detect k(+)-channel-dependent vasodilators. | this study describes a sensitive in vitro relaxation assay using isolated rabbit mesenteric artery to detect the activity of a vasodilator as a k(+)-channel activator. thus, comparison of several known k(+)-channel activators was made with other vasodilators known to work via various cellular mechanisms. the vasodilators used were minoxidil sulfate (mnxs; 5 microm), brl-34915 (cromakalim, 0.1 microm), nicorandil (10 microm), pinacidil (1 microm), diazoxide (100 microm), sodium nitroprusside (10 ... | 1990 | 2292878 |
| [human immunodeficiency virus infection associated with tuberculosis]. | in order to detect an association between hiv infection and tuberculosis (tb), 130 tb inpatients were studied one of whom presented a pulmonary disease due to mycobacterium avium intracellulare. all had advanced tb, 95.4%, with pulmonary localization. serum anti-hiv antibodies were detected by elisa and their presence confirmed by immunoblotting in 4 (3.1%) individuals, three males and one female, with different degrees of pulmonary tb. of the males, 1 was bisexual, 2 were promiscuous, and the f ... | 1990 | 2292908 |
| assay of all-trans----11-cis-retinoid isomerase activity in bovine retinal pigment epithelium. | | 1990 | 2292965 |
| lumbar intrathecal administration of naloxone antagonizes analgesia produced by electrical stimulation of the hypothalamic arcuate nucleus in pentobarbital-anesthetized rats. | in lightly pentobarbital-anesthetized and acutely-prepared rats, electrical stimulation within the arcuate nucleus of the hypothalamus consistently inhibited the tail-flick responses to noxious heating of the tail. the opioid receptor antagonist, naloxone hydrochloride applied intrathecally at the lumbar level, at dose of 20 micrograms, reversed this inhibition without affecting the baseline pain threshold. the same dose of naloxone, applied to the cervical subarachnoid space, had no effect on t ... | 1990 | 2293056 |
| [method of modelling an ulcerative process in the stomach]. | | 1990 | 2293167 |
| patch-clamp recordings on rat cardiac muscle slices. | thin slices (100-200 microns) from cardiac ventricles of newborn (1-14 days old) rats were used for patch-clamp recordings. high resistance seals (greater than 10 gohms) could be obtained on this preparation without enzymatic treatment of tissue. resting potential for cardiac fibres, measured in whole-cell configuration, ranged between -30 and -65 mv. averaged sodium currents and inward rectifying potassium single-channel currents, recorded in cell-attached mode, displayed similar features to th ... | 1990 | 2293201 |
| a sensitive and specific liquid-chromatographic assay for determination of ganciclovir in plasma and urine and its application to pharmacokinetic studies in the rabbit. | a liquid-chromatographic assay for the analysis of ganciclovir in plasma and urine is described. this assay involves the use of acyclovir, an antiviral drug structurally related to ganciclovir, as the internal standard. a two-step sample preparation method is used. after protein is precipitated with acetonitrile and the addition of diethyl ether, ganciclovir and the internal standard are back extracted into a small volume of aqueous ammonium phosphate, taking advantage of their relatively high w ... | 1990 | 2293208 |
| release of human serum albumin from poly(lactide-co-glycolide) microspheres. | human serum albumin (hsa) was encapsulated in a 50:50 copolymer of dl-lactide/glycolide in the form of microspheres. these microspheres were used as a model formulation to study the feasibility of controlling the release of large proteins over a 20- to 30-day period. we show that hsa can be successfully incorporated into microspheres and released intact from these microspheres into various buffer systems at 37 degrees c. a continuous release of the protein could be achieved in physiological buff ... | 1990 | 2293220 |
| preliminary observations on the phenotypes of lymphoid cells in mycosis fungoides using anti-human-thymocyte monoclonal antibodies. | the phenotypes of lymphoid cells from twelve patients with mycosis fungoides (including 5 patients in the tumor stage and 7 in the pretumor stage) were determined immunohistochemically using anti-thymocyte monoclonal antibodies (hit1, hit2, and leu 6). the results showed significant differences between the numbers of hit1 and hit2 positive cells in tumor and pretumor stage lesions, indicating that these two monoclonal antibodies may be of help in the staging of mycosis fungoides and in making a ... | 1990 | 2293225 |
| investigation by amperometric methods of intracellular reduction of 2,6-dichlorophenolindophenol in normal and transformed hepatocytes in the presence of different inhibitors of cellular metabolism. | the reduction of 2,6-dichlorophenolindophenol (dcip) was measured by amperometric methods in morris hepatoma 3924a cells, normal isolated rat hepatocytes and in mitochondria isolated from normal rat liver. the influence of aerobic and anaerobic atmospheres and of various inhibitors of cellular metabolism, especially of the respiratory chain (kcn, nan3, oligomycin), on dcip-reduction were studied using glucose, succinate, beta-hydroxybutyrate, alpha-keto-glutarate and oxalacetate as substrates. u ... | 1990 | 2297512 |
| comparison of a latex agglutination assay and an enzyme-linked immunosorbent assay for detecting cholera toxin. | to determine the pandemic potential of vibrio cholerae, one must demonstrate both the presence of o1 antigen and the production of enterotoxin (ct). tissue culture or enzyme-linked immunosorbent assays (elisas) for ct have been limited to research and reference laboratories. a kit for detecting ct by reversed passive latex agglutination is now commercially available and was used to test 168 strains of v. cholerae o1 and non-o1. when compared with the routine elisa, the latex test was 98% accurat ... | 1990 | 2298870 |
| isolation of vibrio cholerae non-01 from a somerset farmworker and his tropical fish tank. | vibrio cholerae non-01 is occasionally isolated from wounds. to our knowledge, this is the first such case to be reported from the u.k. it was associated with isolation of a similar organism from a tropical fish tank. | 1990 | 2299183 |
| vibrio cholerae bacteremia in a 22-month-old new mexican child. | | 1990 | 2300417 |
| in vitro activity of norfloxacin and other antibacterial agents against gastro-intestinal pathogens isolated in sweden. | the in vitro activity of norfloxacin was compared to that of ampicillin, doxycycline, chloramphenicol, trimethoprim in combination with sulfamethoxazole (1/20), and erythromycin, against 272 clinical isolates of gastro-intestinal pathogens. norfloxacin was the most active compound of those tested with mics in the range 0.004-2 mg/l. concentrations inhibiting 90% of the strains (mic 90) were 0.008 mg/l for vibrio cholerae, 0.016 mg/l for aeromonas hydrophila, 0.032 mg/l for vibrio cholerae non 01 ... | 1990 | 2302351 |
| antisecretory and antiinflammatory properties of bismuth subsalicylate. | the antisecretory properties of pepto-bismol (pb) and its active ingredient, bismuth subsalicylate (bss), were studied in ligated (rabbit and pig) and unligated (rat) intestinal-segment models. when pb was administered to segments before intestinal inoculation with heat-labile (lt) escherichia coli or vibrio cholerae enterotoxins, the inhibition of fluid accumulation was 74%-94% and 60%-91%, respectively. in the pig, the percentages of inhibition by pb of fluid accumulation produced by organisms ... | 1990 | 2305173 |
| behavioral problems in old dogs: 26 cases (1984-1987). | among 26 dogs greater than or equal to 10 years old, the most frequent owner complaints relating to behavioral problems were destructive behavior in the house (n = 10), inappropriate urination or defecation in the house (n = 10), and excessive vocalization (n = 7). the most frequent behavioral diagnoses were separation anxiety (n = 13) and breakdown of housetraining (n = 6). most of the behavioral problems in the 26 dogs began after the dogs reached the age of 10 years, and most of the dogs had ... | 1990 | 2312394 |
| digestibility of cotton plant by-product diets for sheep at two levels of intake. | crossbred wethers (n = 48) were assigned randomly to 1 of 12 experimental diets, arranged in a 3 x 2 x 2 split plot to evaluate three levels of cotton plant by-product (25, 50 and 75% cpb), two levels of naoh treatment of cpb (0 and 4%) and two levels of monensin supplementation (0 and 30 ppm, dm basis) at two intake levels (70 and 90% of ad libitum). intake was a within-lamb variable. ad libitum intake was established over an initial 17-d adjustment period. feed intake and fecal output were rec ... | 1990 | 2312420 |
| experimental non-o group 1 vibrio cholerae gastroenteritis in humans. | in this study, 27 volunteers received one of three non-o group 1 vibrio cholerae strains in doses as high as 10(9) cfu. only one strain (strain c) caused diarrhea: this strain was able to colonize the gastrointestinal tract, and produced a heat-stable enterotoxin (nag-st). diarrhea was not seen with a strain (strain a) that colonized the intestine but did not produce nag-st, nor with a strain (strain b) that produced nag-st but did not colonize. persons receiving strain c had diarrhea and abdomi ... | 1990 | 2312721 |
| epidemic cholera in west africa: the role of food handling and high-risk foods. | during an epidemic of cholera in guinea, west africa, in 1986, the authors conducted two studies of risk factors for transmission. in the capital city, 35 hospitalized cholera patients were more likely than 70 neighborhood-matched controls to have eaten leftover peanut sauces (odds ration (or) = 3.1, 95% confidence interval (ci) 1.2-8.2), but less likely to have eaten tomato sauces (or = 0.2, 95 percent ci 0.1-0.9). hand washing with soap before meals by all family members protected against chol ... | 1990 | 2316500 |
| modulation of tartrate-resistant acid phosphatase expression by calcitriol in csf-induced macrophage colonies. | the osteoclast is thought to be a hemopoietically derived cell, but questions exist about which hemopoietic growth factors are responsible for proliferation of osteoclast precursors. experiments were thus performed to see if recombinant human colony-stimulating factors (csfs) influenced the expression of tartrate-resistant acid phosphatase (trap), an osteoclast marker enzyme, by monkey bone marrow colonies in vitro. in addition, the effect of 1,25-dihydroxyvitamin d3 (calcitriol) on csf-induced ... | 1990 | 2323362 |
| comparative analysis of the hemolysin genes of vibrio cholerae non-01, v. mimicus, and v. hollisae that are similar to the tdh gene of v. parahaemolyticus. | the genes encoding the hemolysins similar to the thermostable direct hemolysin (tdh gene) of vibrio parahaemolyticus were cloned from chromosomes of v. mimicus and v. hollisae. these cloned hemolysin genes and previously cloned tdh genes of v. parahaemolyticus and v. cholerae non-01 were compared by physical mapping and by hybridization with oligodeoxyribonucleotide probes. the nucleotide sequences in the coding regions of all the cloned hemolysin genes were very homologous and had only minor va ... | 1990 | 2323548 |
| production, characterization, and species specificity of monoclonal antibodies to mycobacterium avium complex protein antigens. | the incidence of mycobacterium avium-mycobacterium intracellulare complex infections has increased in recent years primarily because a significant proportion of acquired immunodeficiency syndrome patients develop disseminated m. avium complex disease. in an effort to develop new tools to study these infections, we have produced eight monoclonal antibodies directed against m. avium. western blot (immunoblot) specificity analysis and protease sensitivity assays indicate that four of these antibodi ... | 1990 | 2323822 |
| diminished immunogenicity of a recombination-deficient derivative of vibrio cholerae vaccine strain cvd103. | to address potential concerns over the release of genetically engineered live bacterial vaccines, we constructed a recombination-deficient derivative of the vibrio cholerae o1 vaccine strain cvd103 (cvd103rm). oral immunization of adult volunteers with cvd103rm showed that the reca mutation significantly diminished colonization ability and immunogenicity of the vaccine strain. | 1990 | 2323828 |