| synthesis of disaccharide methyl glycosides related to the polysaccharide from klebsiella serotype 40 and a study of their inhibition in the precipitin reaction. | the methyl glycosides of alpha-d-manp-(1----4)-alpha-l-rhap (3), alpha-l-rhap-(1----3)-beta-d-galp (4), beta-l-rhap-(1----3)-beta-d-galp (5), beta-d-galp-(1----2)-alpha-l-rhap (6), and beta-d-glcpa-(1----2)-alpha-l-rhap (7) have been synthesised and their inhibition reactions in the klebsiella serotype 40 immune system have been studied. the results obtained accord with only one of two structures proposed for the repeating unit of the k40 antigen. | 1990 | 2085817 |
| [preliminary study on serotyping of klebsiella pneumoniae and its clinical significance]. | this paper reports the results of 32 type-specific klebsiella antisera preparations made in our laboratory, through immunizing new zealand rabbits with standard klebsiella strains. the significant titres of these antisera ranged from 1:8-1:256. using above mentioned a antisera, 282 isolates of klebsiella mainly isolated from sputa were typed with the method of coagglutination test. the typeable rate of total isolates was 69.1% (195/282). the most common serotypes were k1 and k33, representing 13 ... | 1990 | 2085862 |
| clinical evaluation of the imipenem/cilastatin combination in the therapy of severe infections in patients with malignant diseases. | thirty hospitalized patients (23 men and 7 women), aged 42 to 78 years, with impaired host defences for malignant underlying diseases and affected by life-threatening infections, were treated with the imipenem/cilastatin combination at a dosage ranging between 2 and 4 g daily i.v. at 8 or 12 h intervals. the average length of therapy was 9.6 +/- 2.09 days. the isolated organisms were as follows: pseudomonas aeruginosa [12], escherichia coli [10], klebsiella pneumoniae [5], proteus mirabilis [3], ... | 1990 | 2086164 |
| outbreak of nosocomial infections due to klebsiella pneumoniae producing shv-4 beta-lactamase. | one hundred and fifty-four clinical isolates of klebsiella pneumoniae resistant to broad-spectrum cephalosporins, aztreonam and amikacin were responsible for an outbreak of nosocomial infections lasting eight months in a university hospital in paris. this outbreak occurred in the intensive care unit (39 patients), haematology units (8 patients) and surgical and medical units (11 patients). antibiotic resistant strains were isolated from the urinary tract (48%), wound and drainage fluids (21%), r ... | 1990 | 2086215 |
| antimicrobial activity and beta-lactamase stability of bmy-28232, parent compound of an oral cephalosporin. | bmy-28232, an aminothiazolyl imino methoxy cephalosporin which is available as an orally absorbed acetoxyethyl ester, inhibited strains of methicillin-susceptible staphylococcus aureus, hemolytic streptococci, streptococcus pneumoniae, escherichia coli, klebsiella species, and many strains of proteus and providencia stuartii at concentrations less than 1 microgram/ml, including isolates resistant to cephalexin and cefaclor. it had activity similar to that of cefixime, but was more active against ... | 1990 | 2086221 |
| [detection of causative bacteria for bovine mastitis and their susceptibility to beta-lactam antibacterial agents]. | during the period from november 1988 to may 1989, causative bacteria in a total of 172 clinical mastitis cases observed in 66 farms in 5 districts with 6 areas in japan were examined and frequencies of their occurrences were determined. susceptibilities (in mics) of the isolates to 6 beta-lactam antibacterial agents were also determined. as a result, coagulase-negative staphylococci (cns) was identified in 94 out of 172 cases (54.7%) and were the most prevalent. corynebacterium spp., gram-negati ... | 1990 | 2086812 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. research group for testing imipenem susceptibility on clinical isolates]. | we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at 459 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were performed at each hospital laboratory and the tests were carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: , ++, + and -. ipm had significantly high activity against st ... | 1990 | 2086814 |
| [growth of coxiella burnetii in selected cell cultures]. | usefulness of monolayer cell cultures for c. burnetii multiplication was evaluated. continuous vero cell line and primary cell (cultures cell culture of monkey kidney cercopithecus aethiops, cell culture of chicken fibroblasts, diploid cell line of human embryo) was inoculated for this purpose. two coxiella burnetii strains suspensions were used as inoculum of standard henzerling strain and zamość strain isolated from an area of poland. primary cell lines were characterized by considerable degre ... | 1990 | 2087134 |
| [catabolism of amino acids in bacteria of the genus klebsiella]. | propagation and activity level of 18 enzymes catalyzing deamination reactions of dicarboxylic and oxyamino acids and enzymes of amino acid reamination and amino acid n-acyl-derivatives' deacylation have been studied in klebsiella bacteria. klebsiella the most actively utilizes serin, threonine, aspartic and glutamic acids and aromatic amino acids. the first three amino acids are utilized by deamination, aromatic acids- in aminotransferase reaction with alpha-ketoglutaric acid, glutamic acid--by ... | 1990 | 2087795 |
| nucleotide sequence of the shv-5 beta-lactamase gene of a klebsiella pneumoniae plasmid. | the nucleotide sequence of the shv-5 beta-lactamase gene, subcloned from a plasmid of klebsiella pneumoniae, was determined. the amino acid changes thought to be responsible for the extended substrate profile of shv-5 are gly----ser234 and glu----lys235. shv-5 is identical to shv-4, except for leu----arg201, which accounts for the difference in apparent pi of the two enzymes. | 1990 | 2088203 |
| [klebsiella pneumoniae capsular type 48 isolated for the first time in turkey]. | capsule type 48 klebsiella pneumoniae strains were isolated from cerebrospinal fluid and blood specimens of 4 days old baby. this type of klebsiella were the first observation in turkey. | 1990 | 2089236 |
| [klebsiella pneumoniae capsular type 25 and type 16 isolated for the first time in turkey]. | klebsiella pneumoniae capsule type 25 and 16 strains were isolated from the urines of two cases having urinary tract infection. these capsule types of klebsiella were the first observation in turkey. | 1990 | 2089237 |
| [klebsiella pneumoniae (kp) bacteremia associated with early gastric carcinoma]. | | 1990 | 2090179 |
| [solitary splenic abscess cured after percutaneous drainage]. | | 1990 | 2090240 |
| [anomalous images in the retroperitoneal space]. | | 1990 | 2091077 |
| klebsiella pneumoniae induced osteomyelitis of the mandible. | | 1990 | 2092073 |
| [ciprofloxacin in the treatment of infections in oncology patients]. | treatment of infectious complications with ciprofloxacin in 65 patients provided good and satisfactory results in 67.7 and 20.0 per cent of the cases, respectively. the drug was efficient in sepsis, urogenital infections, respiratory infections and postoperative purulent complications. ciprofloxacin showed a broad antibacterial spectrum. 96.3 per cent of the isolates belonging to aerobic organisms causing purulent inflammatory processes, including those with high antibiotic resistance levels, su ... | 1990 | 2092640 |
| combination of ofloxacin and other antimicrobial agents. | we evaluated the combination of ofloxacin with piperacillin, gentamicin, vancomycin, rifampin, clindamycin, and metronidazole by the checkerboard agar dilution method against 165 isolates which included escherichia coli, klebsiella pneumoniae, enterobacter cloacae, serratia marcescens, pseudomonas aeruginosa, providencia stuartii, acinetobacter, staphylococcus aureus, bacteroides fragilis, and citrobacter perfringens. synergy of piperacillin-ofloxacin was demonstrated for 50% and 40% respectivel ... | 1990 | 2093103 |
| [neonatal osteomyelitis. study of a series of 35 cases]. | after a brief literature review, we analyze the results obtained with a retrospective study of 35 neonatal osteomyelitis diagnosed between 1-january-75 and 31-december-87. the valuated frequency was of 0.40% alive newborns. between the antecedents, we find previous neonatal sepsis in 68% of the cases. the clinical general findings were less apparent, emphasizing among the local symptoms the pain to passive mobilization and swelling. from acute phase reactants, this study rebounds the high sensit ... | 1990 | 2096756 |
| impact of the antibiotic dosage schedule on efficacy in experimental lung infections. | in agreement with results obtained in the thigh infection model for which there is no human equivalent, the impact of the dosage schedule on efficacy in various clinically relevant experimental lung infections is related to the class of antibiotic. the efficacy of beta-lactams increases with increasing frequency of administration. sustained antibiotic concentrations in serum at a relatively low level are more effective than high peak concentrations at intervals. in contrast, the dosing interval ... | 1990 | 2097704 |
| in vivo efficacy of cefcanel daloxate in comparison with cefaclor. | cefcanel daloxate is a new prodrug cephalosporin for oral use, with cefcanel as the antibacterially active principle. the in vivo efficacy of this prodrug in comparison with cefaclor was assessed in a localized thigh infection model in mice. mice were infected intramuscularly in the thigh and treated orally with cefcanel daloxate or cefaclor with three doses given 70 min apart. one strain each of s. aureus, escherichia coli and klebsiella pneumoniae was used as test organism. the first dose was ... | 1990 | 2097707 |
| [a hygienic study of new raw materials for cosmetic agents and household chemical preparations]. | studies are performed on the new bulgarian raw materials "aminookis" (ao) and "dezodorirashch agent" (da) the plants "alen mak"--plovdiv and "hyaluron"--sofia. chemical, toxicological and microbiological studies are carried out. thin-layer chromatographic and spectrophotometric methods for determination of ao, hyaluronic acid and da are developed and chemical studies on cosmetics, prepared with them, are carried out. during the repetition of a 21-day experiment on guinea pigs the threshold irrit ... | 1990 | 2099460 |
| pharmacology of cefminox, a new bactericidal cephamycin. | cefminox is a new cephamycin antibiotic possessing a d-amino acid moiety derived from d-cysteine at the c-7b side chain. cefminox is active against a wide range of bacteria, especially gram-negative and anaerobic bacteria. cefminox shows excellent in vivo efficacy (ed50) which is higher than would be expected from its in vitro activity (mic). moreover, cefminox possesses more potent activity in suppression of bacterial regrowth than other cephems. these phenomena are surmised to be caused by str ... | 1990 | 2100248 |
| bacterial agents associated with bronchopulmonary disorders in eastern nigeria. | altogether 16,539 sputum specimens were examined microbiologically from 1980 to 1984. out of these 12,588 were screened by ziehl-neelsen's staining technique and 782 were (6.3%) found afb-positive. age and sex distributions of the afb-positive individuals were statistically significant (at 0.05), incidence being most prevalent among those 20 years and above (90.2%) and among males (61.2%). from other specimens cultured, non-afb organisms were isolated at the following frequencies: coliform-like ... | 1990 | 2101201 |
| clinical experience with pefloxacin in patients with urinary tract infections. | a total of 27 patients, 21 of them females, with a median age of 56 were treated with pefloxacin for urinary tract infections. some 74% of these infections were associated with upper tract symptoms, and escherichia coli was the most common causative micro-organism (75% of cases). fifteen patients had co-existent diseases with/without urological abnormalities. all strains of e coli were sensitive to pefloxacin; actinobacter and one strain of klebsiella and streptococcus faecalis were resistant. t ... | 1990 | 2102145 |
| [etiopathogenesis of the inflammation process and the incidence of urinary tract infections]. | etiopathogenesis of inflammatory processes of the urinary tract, mainly of obstructive uropathy and chronic pyelonephritis, is reviewed. the most frequent cause of obstruction is renal calculosis; in the pathogenesis of chronic pyelonephritis the following factors are of great importance: renal and urethral anomalies, renal stones, pregnancy, bladder instrumentation as catheterisation, etc. we analyzed 2101 random by selected hospital patients and found significant bacteriuria in 740 (35 p.c.) o ... | 1990 | 2102560 |
| [sepsis at an internal medicine department]. | 185 cases of bacteremia admitted at the internal medicine department of "c.s. virgen de la arrixaga" in murcia from 1977 to 1986, were studied retrospectively. the common infection was significantly associated to staphylococcus aureus, streptococcus pneumoniae, streptococcus a group and neisseria meningitidis and the nosocomial infection was associated to klebsiella pneumoniae, serratia marcescens y pseudomonas aeruginosa, staphylococcus epidermidis and enterobacter. we did not find significant ... | 1990 | 2103194 |
| perforated jejunal diverticulum with multiple hepatic abscesses. | we have described a patient with multiple hepatic abscesses caused by a perforated jejunal diverticulum with a presumed route of infection via the portal vein. patients with hepatic abscesses and no known source of infection should be evaluated for a contained mesenteric perforation of the gastrointestinal tract. finally, in patients who fail to respond promptly to percutaneous catheter drainage of a liver abscess, a continuing source of infection, such as perforation of a jejunal diverticulum, ... | 1990 | 2105531 |
| [infection in acute pancreatitis]. | | 1990 | 2106046 |
| inhibitory and bactericidal activity of cefpirome and cefotaxime against blood culture isolates. | using a broth microtiter dilution method, minimum inhibitory (mic) and minimum bactericidal concentrations (mbc) were determined for the aminothiazolyl cephalosporins cefpirome and cefotaxime against 436 blood culture isolates. at concentrations of less than or equal to 16 mg/l, cefpirome inhibited 82.3% of the isolates and cefotaxime 66.5%. at the same concentrations, cefpirome killed 60.0% of the isolates and cefotaxime 46.7%. whereas the mic values indicated a far better activity of cefpirome ... | 1990 | 2106417 |
| reversible adp-ribosylation is demonstrated to be a regulatory mechanism in prokaryotes by heterologous expression. | the primary product of biological nitrogen fixation, ammonia, reversibly regulates nitrogenase activity in a variety of diazotrophs by a process called "nh4(+)-switch-off/on." strong correlative evidence from work in azospirillum lipoferum and rhodospirillum rubrum indicates that this regulation involves both the inactivation of dinitrogenase reductase by dinitrogenase reductase adp-ribosyltransferase and the reactivation by dinitrogenase reductase activating glycohydrolase. the genes encoding t ... | 1990 | 2106680 |
| new screening test to determine the acceptability of 0.45-micron membrane filters for analysis of water. | during routine membrane filter (mf) quality control testing, irregularities such as partial or complete inhibition of microbial growth at grid lines, abnormal spreading of colonies, growth in or along the grid lines, nonwetting areas, poor colony sheen and metallic sheen on the mf surface with mendo agar, brittleness, decreased recovery, and severe wrinkling were observed with several lots of filters. to study these effects and to develop a more sensitive screening test for mf quality, we compar ... | 1990 | 2106827 |
| in vivo evaluation of a dual-action antibacterial, ro 23-9424, compared to cefotaxime and fleroxacin. | the dual-action antibacterial r 23-9424 (desacetylcefotaxime linked to the quinolone fleroxacin) is a new antibacterial agent with excellent in vitro activity. it was evaluated for in vivo efficacy in comparison with the cephalosporin cefotaxime and the quinolone component, fleroxacin. ro 23-9424 demonstrated significant activity against all strains tested in systemic infections, including those strains resistant in vivo to cefotaxime (staphylococcus aureus 753, serratia marcescens sm and pseudo ... | 1990 | 2107064 |
| rapid presumptive identification of gram-negative rods directly from blood cultures by simple enzymatic tests. | gram-negative rods were presumptively identified directly from blood cultures within 15 min as escherichia coli, a member of the klebsiella-enterobacter group, or oxidase positive. samples of artificially seeded blood cultures (193 cultures) and patient blood cultures (78 cultures) were filtered into a dynadepth test card with the bac-t-screen instrument (vitek, inc., hazelwood, mo.). triton x-100 was then filtered into the test card to lyse the blood cells but not the entrapped bacteria, and ei ... | 1990 | 2107196 |
| microwave: practical cost-effective method for sterilizing urinary catheters in the home. | we used a standard microwave oven to sterilize red rubber catheters used for intermittent self-catheterization. catheters were incubated for sixty minutes in a suspension of microorganisms isolated from the urine of patients with urinary tract infections. for each trial, 6 catheters were removed from their respective suspensions, placed in separate plastic freezer bags, distributed evenly in a microwave oven (avoiding cold spots), and microwaved simultaneously for twelve minutes. a control cathe ... | 1990 | 2107618 |
| effect of klebsiella ozaenae on ciliary activity in vitro: implications in the pathogenesis of atrophic rhinitis. | klebsiella ozaenae is a gram-negative rod that has been isolated with relative frequency from patients with atrophic rhinitis. the relationship of this bacterium to the pathogenesis of atrophic rhinitis is not understood, and whether this bacterium is simply an opportunistic colonizer of the injured nose or the etiologic agent of the disease is unclear. this study was designed to investigate a potential role for bacterially produced cilioinhibition as a mechanism for the pathogenesis of atrophic ... | 1990 | 2108406 |
| further characterization of monoclonal antibodies to lipopolysaccharide of salmonella minnesota strain r595. | we have shown here that despite the use of monoclonal antibodies with well-defined epitope-specificities, and despite testing them in the most simple animal model available (i.e., mixing of homologous lps with mab prior to injection), we are not yet able to explain why some of the antibodies were effective and others not. for some of the clones (e.g., clone 20), an even better definition of binding sites is currently taking place in an attempt to obtain this understanding. we also do not yet und ... | 1990 | 2109505 |
| comparative in vitro activity of several antimicrobial agents against selected clinical isolates. | a comparison between the in vitro activities of ceftazidime, cefotaxime, ceftriaxone, ampicillin, piperacillin, gentamicin, tobramycin and netilmicin was performed on selected clinical isolates. the activity of the agents was assessed by means of minimum inhibitory concentration determinations, and time-kill studies. the third generation cephalosporins were active against all members of the enterobacteriaceae excluding some enterobacter species. their activity against these bacteria was generall ... | 1990 | 2109875 |
| double-blind, dose-range-finding study of fleroxacin (ro 23-6240; am-833) for treatment of complicated urinary tract infections. | in a double-blind randomized trial, we evaluated the efficacy and safety of three oral dosage regimens of fleroxacin, a new fluoroquinolone, once daily in 62 patients for the treatment of complicated urinary tract infections. the regimens compared were 200 mg for 10 days (n = 20), 400 mg for 10 days (n = 21), and 600 mg for 10 days (n = 21). forty-five patients were evaluable for efficacy. a clinical cure was reached in 78% of the patients. overall, a favorable bacteriological response (negative ... | 1990 | 2110437 |
| ph regulation of urease levels in streptococcus salivarius. | potential mechanisms for regulation of urease levels in streptococcus salivarius were examined, including: induction by urea, nitrogen or carbon source repression, and effects of ph and co2 (because co2 enrichment enhanced urease detection on urea agar plates). regulation by either ph or co2 was confirmed by comparison of the urease accumulation pattern during anaerobic growth under co2 with that under n2. under co2, there was an initial buffering plateau at ph 6.2 and a rate of streptococcus sa ... | 1990 | 2110582 |
| mucinophilic and chemotactic properties of pseudomonas aeruginosa in relation to pulmonary colonization in cystic fibrosis. | representative isolates of nonmucoid pseudomonas aeruginosa were studied to investigate the hypothesis that mucinophilic and chemotactic properties in this species act as potential factors in the initial stages of pulmonary colonization in patients with cystic fibrosis (cf). transmission electron microscopy with a surfactant monolayer technique was used in a novel manner to demonstrate the adhesion of all 10 p. aeruginosa strains examined to porcine gastric mucin and tracheobronchial mucin from ... | 1990 | 2111280 |
| the survival and transfer of microbial contamination via cloths, hands and utensils. | survival and transfer of bacteria from laminated surfaces and cleaning cloths were investigated under laboratory conditions. drying produced substantial reductions in numbers of recoverable organisms and achieved satisfactory decontamination of clean laminate surfaces. on soiled surfaces and on clean and soiled cloths, gram-positive and some gram-negative species survived for up to 4 h, and in some cases up to 24 h. where contaminated surfaces or cloths came into contact with the fingers, a stai ... | 1990 | 2111304 |
| effects of incorporating ampicillin, bile salts and carbohydrates in media on the recognition and selection of aeromonas spp. from faeces. | the effects of incorporating ampicillin, some bile salts and sugars into media on the primary recognition and selection of aeromonads from faeces were examined. most (88%) of the 101 aeromonas strains examined had an ampicillin mic of greater than or equal to 40 mg/l, and would be isolated on blood agar containing ampicillin 30 mg/l. the strains with an ampicillin mic of less than 40 mg/l were all of human origin and predominantly a. caviae. although ampicillin at 10, 20 or 30 mg/l in blood agar ... | 1990 | 2111407 |
| the antibiotic sensitivity of bacteria isolated from the blood of patients in st thomas' hospital, 1969-1988. | we have monitored the antibiotic sensitivity of bloodstream isolates of common bacteria over a period of 20 years. among the gram-positive bacteria, the proportion of isolates of staphylococcus aureus resistant to methicillin, erythromycin, fusidate, or gentamicin has increased marginally, while that of coagulase-negative staphylococci (mostly staph. epidermidis) has increased markedly. enterococci are becoming serially more resistant to high concentrations of aminoglycosides. the enterobacteria ... | 1990 | 2112124 |
| [susceptibility of bacteria isolated from patients with lower respiratory tract infections to antibiotics (1987)]. | since 1981, in cooperation with research institutions across the nation, ikemoto, et al. have been collecting clinical isolates from patients with respiratory tract infections and conducting an annual retrospective survey of patients' background factors and of isolated strains and their sensitivities to various antibacterial agents and antibiotics. in the period from october, 1987 to september, 1988, 17 institutions participated in the survey and a total of 706 strains which were demonstrated to ... | 1990 | 2112206 |
| [clinical evaluation of therapy for aspiration pneumonia with imipenem/cilastatin sodium]. | in an open, prospective, multicenter trial the clinical efficacy of imipenem/cilastatin sodium (ipm/cs) for the treatment of 14 cases with aspiration pneumonia was investigated. the mean age was 75.4 years old. diseases of central nervous system were present in 11 cases, cardiovascular diseases, pulmonary diseases and diabetes mellitus in 2 cases each respectively. seven cases were community-acquired and another seven were hospital-acquired. six cases were moderate and 8 cases were severe. causa ... | 1990 | 2112207 |
| comparative in vitro activity of the new quinolone sparfloxacin (ci-978, at-4140) against nosocomial gram-negative bloodstream isolates. | the in vitro activity of sparfloxacin (ci-978, at-4140), a new quinolone which is active against gram-negative and gram-positive bacteria, and nine other broad-spectrum antibiotics was tested against 128 gram-negative nosocomial bloodstream isolates from separate patients. sparfloxacin and ciprofloxacin were among the most potent antibiotics against escherichia coli (n = 40), enterobacter cloacae (n = 18), klebsiella oxytoca (n = 13), and klebsiella pneumoniae (n = 19), with mic90 values of less ... | 1990 | 2112467 |
| [antibiotic sensitivity of the main opportunistic pathogenic aerobic bacteria]. | antibiotic sensitivity of 1421 strains of staphylococcus aureus, staphylococcus epidermidis, escherichia coli, proteus mirabilis, proteus vulgaris, pseudomonas aeruginosa and klebsiella spp. was studied. gentamicin, levomycetin (chloramphenicol) and ristomycin proved to be the antibiotics of choice in treatment of purulent inflammatory diseases caused by s. epidermidis and s. aureus. for antibiotic therapy of infections caused by gram-negative organisms gentamicin and polymixin might be recommen ... | 1990 | 2113800 |
| in vitro activity of cefpirome compared with other third generation cephalosporins against nosocomial isolates in argentina. | the in vitro activity of cefpirome was evaluated against strains that showed conflicting results for third generation cephalosporins. against isolates with derepressed inducible chromosomal cephalosporinase (n = 40) cefpirome was the sole cephalosporin with an mic90 in the susceptible range; klebsiella spp. with plasmid-mediated beta-lactamases (broad spectrum shv-2 or shv-2 type) (n = 40) remained most susceptible to ceftizoxime and cefpirome; against aminoglycoside-resistant pseudomonas aerugi ... | 1990 | 2114372 |
| serum bactericidal activity from intravenous ciprofloxacin and azlocillin given alone and in combination to healthy subjects. | ciprofloxacin plus azlocillin have been shown to exhibit in vitro synergy versus a variety of organisms, including pseudomonas aeruginosa. this study examined this interaction in vivo, testing serum bactericidal activity (sba) in six healthy male subjects after intravenous administration of ciprofloxacin 4 mg/kg (c), azlocillin 60 mg/kg (a), and the two simultaneously (c/a). eight different organisms were tested: four isolates of p. aeruginosa with varying susceptibilities to c and a, and one is ... | 1990 | 2114954 |
| synthesis and biological evaluation of a series of parenteral 3'-quaternary ammonium cephalosporins. | the preparation and biological evaluation of a series of 7 beta-[2-(2-aminothiazol-4-yl)-2(z)-methoximinoacetamido]cep halosporins, substituted at the 3'-position with monocyclic or bicyclic nitrogen-containing heterocycles are described. the resulting family of parenteral compounds displays a broad spectrum of antibacterial activity. some compounds exhibit a similar level of gram-negative activity to that of the "third-generation" cephalosporins with increased staphylococcal activity. the in vi ... | 1990 | 2115587 |
| role of enterobacteria and hla-b27 in spondyloarthropathies: studies with transgenic mice. | several mhc (major histocompatibility complex) genes are associated with increased incidence of disease. the strongest association is between the class i gene, hla-b27, and ankylosing spondylitis (as). hla-b27 is also highly associated with reiter's syndrome. as not all subjects with hla-b27 develop as or reiter's syndrome, environmental factors may have a key role in the pathogenesis of these arthritic diseases. several studies have implicated klebsiella in the development of as, whereas reiter ... | 1990 | 2115764 |
| influence of a bacterial extract on antigen presentation and protection against experimental infections. | although substances known as immunodulators are usually used to stimulate nonspecific immunity, their mode of action is not well understood. in an effort to clarify this mechanism, we investigated the effect of a lyophilized bacterial extract (broncho-vaxom) on experimental infections, on normal or irradiated mice, and on antigen processing. | 1990 | 2117182 |
| quantitative analysis of antimicrobial effect kinetics in an in vitro dynamic model. | variants of the available methods for estimating antimicrobial effect kinetics in an in vitro dynamic model were analyzed. two integral parameters characterizing antimicrobial effect duration (te) and intensity (ie) are suggested to define and analyze the concentration-effect relationships in these models, irrespective of the method of recording. te is defined by the time from the moment of antibiotic administration to the movement when the bacterial count again reaches its initial level. ie is ... | 1990 | 2117416 |
| genetic regulation of formate hydrogenlyase of escherichia coli: role of the fhla gene product as a transcriptional activator for a new regulatory gene, fhlb. | a new gene whose product is required for the production of formate hydrogenlyase (fhl) has been identified in escherichia coli. this gene, termed fhlb, maps between the frda (94.4 min) and argi (96.6 min) genes on the e. coli chromosome and is transcribed in a clockwise direction toward argi. biochemical analysis of an fhlb- mutant, strain se-2011 [phi(fhlb-lacz+)], revealed that the mutant lacks formate dehydrogenase activity associated with fhl (fdh-h) and hydrogenase activity. as a result of ... | 1990 | 2118503 |
| structural and chemical properties of a flavodoxin from anabaena pcc 7119. | structural and chemical properties of a flavodoxin from anabaena pcc 7119 are described. the first 36 residues of the amino-terminal amino acid sequence have been determined and show extensive homology with flavodoxins isolated from other sources. anabaena flavodoxin exhibits a net negative change (-3) in the helix-1 segment as found with other cyanobacterial flavodoxins synechococcus 6301 (anacystis nidulans) and nostoc mac, but in contrast to the net positive charge found in this region in the ... | 1990 | 2119231 |
| microbiological analysis of sepsis complicating non-surgical biliary drainage in malignant obstruction. | cholangitis with sepsis is a common complication of non-surgical biliary drainage and represents a difficult management problem. microbiological data for 18 septic episodes in 15 patients following endoscopic biliary drainage (ebd) and 14 septic episodes in 14 patients following percutaneous transhepatic drainage (ptd) were reviewed. in the ebd group, all 18 patient episodes of sepsis had blood cultures and 10 had bile cultures obtained. in total, 17 gram-negative isolates and 5 gram-positive is ... | 1990 | 2120104 |
| influence of the addition of mineral salts on the inhibitory activity of strains of the arthrinium genus. | the influence which the addition of certain mineral salts has on the inhibiting activity of strains belonging to the arthrinium genus was studied. the salts employed were agno3, fecl2, mncl2 and cuso4, in concentrations of 10, 50, 100, 150 and 200 mg/l, in the presence of bacillus subtilis, enterobacter cloacae, staphylococcus aureus, escherichia coli, serratia marcescens, klebsiella pneumoniae, candida albicans and aspergillus niger. for b. subtilis and s. aureus greater inhibition was observed ... | 1990 | 2120549 |
| percutaneous cholecystostomy drainage for the treatment of acute emphysematous cholecystitis. | | 1990 | 2120927 |
| preliminary studies of the effect of ozone on the bactericidal properties of complement. | the study analyzes the bactericidal influence of an ozonized blood serum on gram-negative bacilli. considerable differences were observed concerning the effect which ozonized normal human serum has upon klebsiella, pseudomonas and salmonella strains. it was found that the impact may vary depending not only on the time of the exposure of serum to ozone, but also on the type of strain and the structure of the outer membrane of the bacteria. the salmonella typhimurium strain of the ra chemotype tre ... | 1990 | 2121415 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from urinary tract infections (1987). i. susceptibility distribution]. | since 1979, ikemoto et al. have been retrospectively surveying the sensitivity of major species of bacteria isolated from patients with urinary tract infections to various antibacterial agents and antibiotics. their findings for the past year are reported below. a total of 825 clinical strains of bacteria was investigated. of this total, gram-positive bacteria accounted for 28.0% (231 strains) and gram-negative bacteria for 72.0% (594 strains). taxonomically, escherichia coli accounted for 34.7% ... | 1990 | 2122040 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from urinary tract infections (1987). iii. secular changes in susceptibility]. | sensitivity spectra of major species of bacteria (namely escherichia coli, klebsiella spp., proteus spp., citrobacter spp., enterobacter spp., serratia marcescens and pseudomonas aeruginosa) which were among the clinical isolates mentioned in the first and second reports to various antibacterial and antibiotic agents and time courses of the spectra are reported below. 1. e. coli was sensitive to cefmenoxime (cmx), latamoxef (lmox), ceftazidime (caz), cefzonam (czon) and flomoxef (fmox) which are ... | 1990 | 2122041 |
| [piperacillin-amikacin combinations: killing curves]. | bactericidal activity as a function of time of piperacillin (pip) and amikacin (akn) alone and in combination was evaluated by killing curves technique on 23 clinical isolates: e. coli (6), k. pneumoniae (5), e. cloacae (6) and p. aeruginosa (6), for which the minimal inhibitory concentrations ranges of piperacillin were 0.25 to 64 mg/l and of amikacin 1 to 8 mg/l. for each species, the strains were chosen according to the most frequent phenotypes: beta-lactams susceptible, penicillinase (pase), ... | 1990 | 2122398 |
| quantitative changes in faecal microflora preceding necrotising enterocolitis in premature neonates. | quantitative studies of faecal bacterial flora were carried out during the week preceding the clinical onset of 12 episodes of neonatal necrotising enterocolitis. there were considerable quantitative changes in the faecal flora preceding the clinical onset of both definite and possible episodes of necrotising enterocolitis. there was a decline in the numbers of some species from up to 72 hours before the clinical onset of the disease. enterobacteriaceae were isolated from samples collected durin ... | 1990 | 2122814 |
| studies of the subgingival microflora in patients with acquired immunodeficiency syndrome. | two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ... | 1990 | 2123926 |
| bactericidal efficacy of 5 per cent povidone iodine cream in pseudomonas aeruginosa burn wound infection. | a new topical antiseptic agent, 5 per cent polyvinylpyrrolidone-iodine (pvp-i) cream, with altered physicochemical properties, incorporated in a different carrier base has proved in vivo to be more effective in controlling burn wound infections than 10 per cent pvp-i ointment. important biodynamic properties of the new formulation have not, however, been elucidated in vivo. hence the need for a controlled study to evaluate the bioavailability of the active component after penetration through bur ... | 1990 | 2124125 |
| synthesis and antibacterial activity of 7 beta-[1-(2-aminothiazol-4-yl)-1-cyclopropanecarboxyamido]cephem derivatives. | the synthesis and antibacterial activity of several 7 beta-[1-(2-aminothiazol-4-yl)-1-cyclopropanecarboxyamido]cep hem derivatives (1) are described. the structure-activity relationships of 1 are also presented. | 1990 | 2124209 |
| [an in vitro study on combination effect of isepamicin and beta-lactam antibiotics]. | a study was done on the combined actions of an aminoglycoside, isepamicin (isp), and 3 beta-lactam antibiotics (cefoperazone (cpz), latamoxef (lmox) and imipenem/cilastatin sodium (ipm/cs] against clinical isolates of pseudomonas aeruginosa, serratia marcescens and klebsiella pneumoniae. minimal inhibitory concentrations of individual antibiotics were compared first. isp and ipm/cs had strong antibacterial activities against all 3 bacterial species while the antibacterial activities of cpz again ... | 1990 | 2124634 |
| inhibitory activity of papain on facultative pathogens--short communication. | papain, a proteolytic enzyme obtained from papaya was found inhibitory on few facultative pathogens. while escherichia coli, staphylococcus albus, klebsiella pneumoniae and enterobacter cloacae were inhibited at concentration from 200 ppm on the cell density of staphylococcus faecalis decreased only at higher concentration of papain (400-800 ppm). | 1990 | 2124762 |
| protein secretion in gram-negative bacteria: transport across the outer membrane involves common mechanisms in different bacteria. | the xcp genes are required for protein secretion by pseudomonas aeruginosa. they are involved in the second step of the process, i.e. the translocation across the outer membrane, after the exoproteins have reached the periplasm in a signal peptide dependent fashion. the nucleotide sequence of a 2.5 kb dna fragment containing xcp genes showed at least two complete open reading frames, potentially encoding proteins with molecular weights of 41 and 19 kd. products with these apparent molecular weig ... | 1990 | 2124971 |
| comparative susceptibility studies of clinically isolated pseudomonas aeruginosa, escherichia coli and klebsiella spp to cefoperazone and cefotaxime. | the in-vitro activity of cefotaxime and cefoperazone were compared using clinically isolated escherichia coli, klebsiella spp and pseudomonas aeruginosa. cefotaxime was found on a weight to weight basis, to be much more active than cefoperazone. all the three species studied show the presence of cefoperazone-resistant population which were sensitive to cefotaxime. the possible mechanisms of resistance to these antibiotics were discussed. | 1990 | 2125616 |
| beta-lactamase in gram-negative rods: the relationship between penicillinase and r plasmids in gram-negative rods. | concomitant with the extensive use of antibiotics, the number of multiple antibiotic-resistant strains has been increasing. since resistance is mainly mediated by r plasmids, we undertook to investigate the characteristics of r plasmid-determined beta-lactamase in 6 gram-negative rods. the beta-lactamase produced by each organism was classified by its substrate: type p which attacks penicillins, type c which attacks cephalosporins, and type c/p which attacks both penicillins and cephalosporins. ... | 1990 | 2126540 |
| [experimental development of aseptic adhesive films]. | antibacterial activity of surgical adhesive films containing various antiseptics in the adhesive layer was studied experimentally. it was shown that the adhesive films containing 0.1 to 1% (in terms of active iodine) of iodopyrone had a stable bactericidal action and were effective against to the main causative agents of surgical infections, i. e. staphylococcus spp., e. coli, klebsiella spp., pseudomonas aeruginosa and others. | 1990 | 2126704 |
| [studies of polymicrobial infection on pneumonia by transtracheal aspiration in the elderly]. | we studied the polymicrobial infection by transtracheal aspiration (tta) on pneumonia in the elderly. incidence of mixed isolation of organisms from tta was 35% or 39 out of 111 episodes. the principal organisms in polymicrobial infection detected from tta were s. aureus (n.23), p. aeruginosa (n. 15), k. pneumoniae (n. 12) and s. pneumoniae (n. 11). the principal combination of mixed infection were s. aureus + p. aeruginosa (n.7), s. aureus + k. pneumoniae (n.5), s. aureus + s. marcescens (n.5), ... | 1990 | 2126799 |
| the frequency of bacterial pathogens in infections potentially preventable by antimicrobial prophylaxis. | bacterial pathogen frequency was analyzed over a fourteen year period at the university of utah medical center. isolation techniques and identification procedures have remained essentially the same during this period, allowing for a valid comparison of this frequency. for most organisms the frequency of overall isolation had remained relatively stable. differences were seen in the frequency of pseudomonas aeruginosa and in the isolation rate of staphylococcus aureus. escherichia coli became prop ... | 1990 | 2126891 |
| growth inhibition of selected food-borne bacteria, particularly listeria monocytogenes, by plant extracts. | six extracts from chinese medicinal plants: tin men chu, sey lau pai, siu mao heung, bak tao yung, kam chin chiu and liao ya, were tested for their inhibitory effect on selected food-borne bacteria by the well assay technique. among them, tin men chu, siu mao heung and sey lau pai inhibited the growth of staphylococcus aureus, klebsiella pneumonia, escherichia coli, shigella flexneri, streptococcus faecalis, salmonella paratyphi, salm. enteritidis, enterobacter aerogenes, pseudomonas fluorescens ... | 1990 | 2127264 |
| susceptibility to ciprofloxacin of nosocomial gram-negative bacteria and staphylococci isolated in the uk. | at the end of 1989 the division of hospital infection of the central public health laboratory completed five years of continuous surveillance of ciprofloxacin susceptibility amongst strains of staphylococcus aureus and pseudomonas aeruginosa. the strains studied were referred from many different uk laboratories for epidemiological or other typing. between 1987 and 1989, referred cultures of coagulase-negative staphylococci, klebsiella spp. and enterobacter spp. were also examined. in total, 25,7 ... | 1990 | 2127270 |
| in vitro activity of sparfloxacin (ci-978; at-4140) against clinical isolates from cancer patients. | the in vitro activity of sparfloxacin, a new quinolone, was compared with those of ciprofloxacin and fleroxacin against gram-positive and gram-negative bacteria, greater than 90% of which were isolated from blood culture specimens of cancer patients. sparfloxacin was extremely active against acinetobacter species, aeromonas hydrophila, citrobacter diversus, enterobacter species, escherichia coli, klebsiella species, proteus vulgaris, and serratia marcescens (inhibiting greater than 90% of these ... | 1990 | 2127348 |
| in-vitro activity of cefodizime against respiratory pathogens. | the in-vitro activity of cefodizime was studied against respiratory pathogens and was compared with the activity of other beta-lactams, ciprofloxacin and erythromycin. cefodizime displayed high activity against haemophilus influenzae (mic90 0.008 mg/l), branhamella catarrhalis and streptococcus pneumoniae (mic90 0.5 mg/l). klebsiella spp. were less susceptible (mic90 2 mg/l). it was concluded that cefodizime should be a useful agent in the therapy of respiratory tract infections. | 1990 | 2127417 |
| [bacteriologic control of total parenteral nutrition formulations at the bellvitge hospital]. | septic complications often lead to halting nutritional parenteral therapy. in general, the source of infection lies in the mixture, the connection and/or point of catheter insertion. the objective of this study is to show the need for bacteriological control throughout the parenteral nutrition process in order to assess the efficacy of the methods used, discover the source of infection and establish an in-house quality control. different methods for bacteriological control of parenteral nutritio ... | 1990 | 2127706 |
| glycosidase activities in alveolar macrophages from guinea pigs stimulated with a glyco-proteic complex extracted from klebsiella pneumoniae. | an increased resistance of laboratory animals to pulmonary infections following per os administration of a glyco-proteic complex extracted from klebsiella pneumoniae has been reported. this was associated with an increased phagocytic capacity of alveolar macrophages (am). in this report, the effect of treating guinea pigs with this extract on the alveolar macrophage (am) glycosidase machinery has been studied. am were collected by bronchoalveolar lavage, the cells were pelleted by centrifugation ... | 1990 | 2128166 |
| [pathogenic and clinical changes of pneumonia in the recent 10 years]. | the article reviewed 279 cases of pneumonia which came from two different periods. among these 116 got from 1960s and 174, from 1980s. we analyzed the general condition of patients, etiology, mortality, prognosis and management and reached to the conclusion that (1) gram-negative bacilli become major pathogen in replace of gram-positive staphylococcus aureus in 1980s, but staphylococcus aureus pneumonia is still a serious disease (2) the prognosis of the patient is related to underlying disease, ... | 1990 | 2128219 |
| [antimicrobial activities of gentamicin against fresh clinical isolates]. | antimicrobial activities of gentamicin (gm), compared with activities of other aminoglycosides (ags) and beta-lactam antibiotics, were studied against clinical isolates obtained during a period of july-december 1989. 1. gm-resistant strains were noted in 24% of staphylococcus aureus, 12% of enterobacter spp., 24% of serratia marcescens, 7% of morganella morganii and 26% of pseudomonas aeruginosa, but no gm-resistant strains were observed among isolates of escherichia coli, klebsiella pneumoniae, ... | 1990 | 2128340 |
| pharmacodynamics of beta-lactam antibiotics on gram-negative bacteria: initial killing, morphology and postantibiotic effect. | the aim of this study was to investigate the pharmacodynamics of beta-lactam antibiotics on gram-negative bacteria by studying the in vitro postantibiotic effect (pae), initial killing and morphology. the pae of aztreonam, cefotaxime, ceftazidime, imipenem, mecillinam and piperacillin on enterobacter cloacae, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and serratia marcescens was studied by use of bioluminescence assay of bacterial atp. the strains were exposed for 2 h to the ... | 1990 | 2129063 |
| interactions of antimicrobial combinations in vitro: the relativity of synergism. | interactions of combinations of netilmicin, amikacin, piperacillin, imipenem, azlocillin, ceftazidime or moxalactam were studied in vitro against pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae and staphylococcus aureus. microtiter checkerboard technique was compared with standard killing curve method and with killing curves obtained in kinetic in vitro models mimicking single or multiple dosing regimens according to human pharmacokinetics. antibiotic combinations were classified ... | 1990 | 2129067 |
| the postantibiotic effect induced by antimicrobial combinations. | antimicrobial combinations are frequently needed for the successful treatment of serious infections. generally, the same dosing schedules are employed irrespective of whether the drugs are used singly or in combination. a postantibiotic effect (pae) has been described for all antibiotics used singly against gram-positive cocci, but only for non-beta-lactams against gram-negative bacilli with the exception of carbapenems against pseudomonas aeruginosa. the major clinical relevance of the pae pert ... | 1990 | 2129068 |
| complex typing of pseudomonas aeruginosa strains isolated in an intensive care unit [a note]. | a total of 2637 gram-negative facultatively pathogenic bacteria were isolated in the lászló central hospital for infectious diseases during a 5-year-period from clinical samples of patients of the respiratory intensive care unit; 28 further strains were cultured from hospital personnel and fomites. pseudomonas aeruginosa, klebsiella and acinetobacter spp. were most frequently isolated. complex typing (determination of o serogroup, phage pattern, pyocin type, antibiogram and plasmid pattern) of p ... | 1990 | 2129258 |
| systematic generation of antigen specific human monoclonal antibodies with therapeutical activities using active immunization. | presenting a panel of human hybridomas secreting serospecific antibodies which confer a high degree of protection against fatal infection with pseudomonas aeruginosa, we report an efficient approach for a systematic generation of antigen specific human monoclonal antibodies with biological activity. this approach is based on active immunization and antigen specific panning. individuals were immunized with polysaccharides isolated from lps of pseudomonas aeruginosa conjugated to toxin a. specific ... | 1990 | 2129420 |
| radio-immunoassay for formyl methionyl leucyl phenylalanine. i. development and application to assessment of chemotactic peptide production by enteric bacteria. | bacterial chemotactic peptides are low molecular weight peptides which stimulate a wide range of neutrophil functions following binding to specific leucocyte receptors. formyl methionyl leucyl phenylalanine (fmlp) is the major chemotactic peptide in escherichia coli culture supernatants. this paper reports the development and validation of a radio-immunoassay (ria) for fmlp and its application to the analysis of formyl peptide production by enteric bacteria in vitro. the assay was moderately sen ... | 1990 | 2129421 |
| antimicrobial spectrum of lignin-related pine cone extracts of pinus parviflora sieb. et zucc. | pine cones of pinus parviflora sieb., et zucc, were extracted successively with 5% nahco3, 3% nh4oh, 1% naoh and 4% naoh, and the extracts were tested for ability to induce antimicrobial activity in mice infected with staphylococcal aureus. escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, candida albicans, or salmonella enteritidis. these alkaline extracts were potent against the first 5 of these strains of microorganisms, and the activity was recovered mostly from their acid-pre ... | 1990 | 2129479 |
| five additional genes in the pulc-o operon of the gram-negative bacterium klebsiella oxytoca unf5023 which are required for pullulanase secretion. | dna sequence analysis, tnpho and tntac-1, mutagenesis, deletion analysis, expression under bacteriophage t7 gene 10 promoter control, subcellular fractionation and complementation tests were used to study the function of dna located in the centre of the pulc-o operon from klebsiella oxytoca strain unf5023. the characterized region of the operon includes five genes (pulg, pulh, puli, pulj and pulk) coding for apparently integral inner membrane proteins which are required for pullulanase secretion ... | 1990 | 2129543 |
| correlation between disc potency and zone size in temafloxacin activity in vitro. | a multi-test disc potency evaluation of a new fluoroquinolone, temafloxacin, has been prepared in order to evaluate the variation of the zone dimension with the disc antibiotic concentration. a direct correlation between these parameters has been demonstrated. all pathogens have been isolated in hospitalized patients. | 1990 | 2130003 |
| the rcsa gene from erwinia amylovora: identification, nucleotide sequence, and regulation of exopolysaccharide biosynthesis. | rcsa is a positive activator of extracellular polysaccharide synthesis in the enterobacteriaceae. a cosmid clone containing the rcsa gene from erwinia amylovora was identified by its ability to restore mucoidy to an e. stewartii rcsa mutant. the rcsa gene was subcloned on a 2.2-kilobase hindiii-psti fragment that hybridized with an e. stewartii rcsa probe and complemented e. stewartii and escherichia coli rcsa mutants. in addition, the cloned e. amylovora rcsa gene stimulated expression of cps:: ... | 1990 | 2131100 |
| is candidiasis the true cause of vulvovaginal irritation in women with diabetes mellitus? | vulvovaginitis is common in diabetic women and is often treated with antifungal agents on the assumption that the causative organism is candida albicans. in a survey of 100 consecutive diabetic women attending a diabetes clinic 36 had complained to their general practitioner about vulvovaginal irritation during the past three years and 26 were treated with antifungal agents without a vaginal examination or swabs being taken. in a separate study 27 post-menopausal women with non-insulin dependent ... | 1990 | 2131794 |
| antibacterial properties of dycal and visible-light-cured dycal (prisma vlc dycal). | | 1990 | 2132224 |
| klebocine typing of klebsiella species isolated from diarrhoeal children under 5 years in madras, india. | klebocine typing of klebsiella isolated as single pathogen from diarrhoeal diseases in children under five years revealed prominent type 4143 (14.8%) and 3322 (12.9%). there was no seasonal variation noticed. | 1990 | 2132496 |
| infective endocarditis after open heart surgery. | this work was done in ain shams university hospital during the period from december 1988 to july 1989. this included 20 patients subjected to open heart surgery and suffering from post operative non reactive fever clinically diagnosed as infection endocarditis. the frequency of positive blood culture endocarditis following open hear surgery was 2.5% and the most affected valve in our study was prosthetic aortic valve (60%). pseudomonas aeruginosa represented 60% of the isolated organisms from bl ... | 1990 | 2133899 |
| [cooperation between the urologist and microbiologist in the diagnosis and treatment of sepsis. ii. the most common microbial species and their sensitivity to antimicrobial drugs in sepsis at the urologic clinic of the medical school hospital]. | the authors investigated the spectrum of aetiological agents and their sensitivity to antimicrobial substances in 52 patients with septicaemia at the urological clinic in hradec králové during 1984-1987. they found a predominating number of gram-negative bacteria (82%). the most frequent agent producing sepsis was klebsiella pneumoniae (19x), its source were in 52% the urinary pathways and 16% the cannula in the subclavian vein. in nine instances the aetiological agent was escherichia coli and 5 ... | 1990 | 2136435 |
| mr imaging of thoracic and abdominal wall infections: comparison with other imaging procedures. | infections of the chest and abdominal wall are rare but potentially fatal disorders that can occur spontaneously or in association with diabetes mellitus, immunosuppression, or trauma. the condition (either in the form of necrotizing fasciitis and/or pyomyositis) is difficult to diagnose clinically because of poor localizing signs. prognosis depends on early recognition, extent of disease, and type of causative organism. pathologically, the infections can manifest as cellulitis, abscess, and/or ... | 1990 | 2138843 |
| purification, characterization, and in vivo reconstitution of klebsiella aerogenes urease apoenzyme. | urease was purified from recombinant klebsiella aerogenes which was grown in the absence of nickel. the protein was inactive and contained no transition metals, yet it possessed the same heteropolymeric structure as native enzyme, demonstrating that ni is not required for intersubunit association. ni did, however, substantially increase the stability of the intact metalloprotein (tm = 79 degrees c) compared with apoenzyme (tm = 62 degrees c), as revealed by differential scanning calorimetric ana ... | 1990 | 2142939 |