| secondary metabolites as co-markers in the taxonomy of aspergilli. | the production of extracellular and intracellular secondary metabolites by 13 aspergillus species has been directly detected using the agar plug technique. in addition to several unknown compound a. aculeatus, a. ochraceus and a. terreus were found to be the highest producers of extracellular secondary metabolites, while the lowest producer species were a. tamarii, a. rugulosus in addition to a. oryzae whose secondary metabolites couldn't be detected by this technique. in case of intracellular s ... | 2003 | 12916724 |
| mining microorganism est databases in the quest for new proteins. | microorganisms with large genomes are commonly the subjects of single-round partial sequencing of cdna, generating expressed sequence tags (ests). usually there is a great distance between gene discovery by est projects and submission of amino acid sequences to public databases. we analyzed the relationship between available ests and protein sequences and used the sequences available in the secondary database, clusters of orthologous groups (cog), to investigate ests from eight microorganisms of ... | 2003 | 12917813 |
| relationships between the ethanol utilization (alc) pathway and unrelated catabolic pathways in aspergillus nidulans. | the ethanol utilization pathway in aspergillus nidulans is a model system, which has been thoroughly elucidated at the biochemical, genetic and molecular levels. three main elements are involved: (a) high level expression of the positively autoregulated activator alcr; (b) the strong promoters of the structural genes for alcohol dehydrogenase (alca) and aldehyde dehydrogenase (alda); and (c) powerful activation of alcr by the physiological inducer, acetaldehyde, produced from growth substrates s ... | 2003 | 12919319 |
| the effect of crea in glucose and xylose catabolism in aspergillus nidulans. | the catabolism of glucose and xylose was studied in a wild type and crea deleted (carbon catabolite de-repressed) strain of aspergillus nidulans. both strains were cultivated in bioreactors with either glucose or xylose as the sole carbon source, or in the presence of both sugars. in the cultivations on single carbon sources, it was demonstrated that xylose acted as a carbon catabolite repressor (xylose cultivations), while the enzymes in the xylose utilisation pathway were also subject to repre ... | 2004 | 12920487 |
| glycosphingolipids of the model fungus aspergillus nidulans: characterization of gipcs with oligo-alpha-mannose-type glycans. | aspergillus nidulans is a well-established nonpathogenic laboratory model for the opportunistic mycopathogen, a. fumigatus. some recent studies have focused on possible functional roles of glycosphingolipids (gsls) in these fungi. it has been demonstrated that biosynthesis of glycosylinositol phosphorylceramides (gipcs) is required for normal cell cycle progression and polarized growth in a. nidulans (cheng, j., t.-s. park, a. s. fischl, and x. s. ye. 2001. mol. cell biol. 21: 6198-6209); howeve ... | 2003 | 12923229 |
| mitochondrial rnase p rnas in ascomycete fungi: lineage-specific variations in rna secondary structure. | the rna subunit of mitochondrial rnase p (mtp-rna) is encoded by a mitochondrial gene (rnpb) in several ascomycete fungi and in the protists reclinomonas americana and nephroselmis olivacea. by searching for universally conserved structural elements, we have identified previously unknown rnpb genes in the mitochondrial dnas (mtdnas) of two fission yeasts, schizosaccharomyces pombe and schizosaccharomyces octosporus; in the budding yeast pichia canadensis; and in the archiascomycete taphrina defo ... | 2003 | 12923256 |
| tina interacts with the nima kinase in aspergillus nidulans and negatively regulates astral microtubules during metaphase arrest. | the tina gene of aspergillus nidulans encodes a protein that interacts with the nima mitotic protein kinase in a cell cycle-specific manner. highly similar proteins are encoded in neurospora crassa and aspergillus fumigatus. tina and nima preferentially interact in interphase and larger forms of tina are generated during mitosis. localization studies indicate that tina is specifically localized to the spindle pole bodies only during mitosis in a microtubule-dependent manner. deletion of tina alo ... | 2003 | 12925754 |
| antifungal agents from the roots of cudrania cochinchinensis against candida, cryptococcus, and aspergillus species. | bioassay-guided fractionation resulted in the isolation of four antifungal agents from the roots of cudrania cochinchinensis. two of these were new compounds, cudraxanthone s [1, 1,3,5,6-tetrahydroxy-2-(1,1-dimethyl-2-propenyl)xanthone] and cudraflavanone b (2, 2',4',5,7-tetrahydroxy-6-prenylflavanone). the remaining two compounds were known compounds, toxyloxanthone c (3) and wighteone (4). among these compounds, 1, 3, and 4 exhibited antifungal activities against cryptococcus neoformans, asper ... | 2003 | 12932139 |
| branching is coordinated with mitosis in growing hyphae of aspergillus nidulans. | filamentous fungi like aspergillus nidulans can effectively colonize their surroundings by the formation of new branches along the existing hyphae. while growth conditions, chemical perturbations, and mutations affecting branch formation have received great attention during the last decades, the mechanisms that regulates branching is still poorly understood. in this study, a possible relation between cell cycle progression and branching was studied by testing the effect of a nuclei distribution ... | 2003 | 12948510 |
| genomics reveals sexual secrets of aspergillus. | | 2003 | 12949156 |
| red fluorescent protein (dsred2) as a novel reporter in fusarium oxysporum f. sp. lycopersici. | pan-dsred2 vector was constructed for constitutive cytoplasmic expression of the red fluorescent protein (dsred2) under control of the glyceraldehyde-3-phosphate dehydrogenase gene promoter from aspergillus nidulans. dsred2-transformation of two fusarium oxysporum f. sp. lycopersici strains pathogenic against tomato host resulted in bright red cytoplamic fluorescence of the fungus. the transformants were screened based on the hygromycin b resistance, brightness, stability and rate of appearance ... | 2003 | 12951257 |
| transcriptional regulation of methionine synthase by homocysteine and choline in aspergillus nidulans. | roles played by homocysteine and choline in the regulation of ms (methionine synthase) have been examined in fungi. the aspergillus nidulans meth gene encoding ms was cloned and characterized. its transcription was not regulated by methionine, but was enhanced by homocysteine and repressed by choline and betaine. ms activity levels were regulated in a similar way. the repression by betaine was due to its metabolic conversion to choline, which was found to be very efficient in a. nidulans. betain ... | 2003 | 12954077 |
| production and secretion of aspergillus nidulans catalase b in filamentous fungi driven by the promoter and signal peptide of the cladosporium fulvum hydrophobin gene hcf-1. | we describe here the use of sequences from the hydrophobin gene hcf-1 of cladosporium fulvum to construct pcatbex, a vector for high-level expression and secretion of catb, a catalase from aspergillus nidulans. transformation of c. fulvum with pcatbex results in a 60-fold increase in the mycelial activity in the fungus and the appearance of up to 5.4 mkat/l of catalase in the growth medium. the levels of catalase in the supernatant increased dramatically following removal of nitrogen from the me ... | 2003 | 12955453 |
| protein prenyltransferases: anchor size, pseudogenes and parasites. | lipid modification of eukaryotic proteins by protein prenyltransferases is required for critical signaling pathways, cell cycle progression, cytoskeleton remodeling, induction of apoptosis and vesicular trafficking. this review analyzes the influence of distinct states of sequential posttranslational processing that can be obtained after single or double prenylation, reversible palmitoylation, proteolytic cleavage of the c-terminus and possible reversible carboxymethylation. this series of modif ... | 2003 | 12956414 |
| homologous expression of a mutated beta-tubulin gene does not confer benomyl resistance on trichoderma virens. | to clone the beta-tubulins and to induce resistance to benzimidazoles in the biocontrol fungus trichoderma virens through site-directed mutagenesis. | 2003 | 12969302 |
| the sclerotinia sclerotiorum pac1 gene is required for sclerotial development and virulence. | the synergistic activities of oxalic acid and endopolygalacturonases are thought to be essential for full virulence of sclerotinia sclerotiorum and other oxalate-producing plant pathogens. both oxalic acid production and endopolygalacturonase activity are regulated by ambient ph. since many gene products with ph-sensitive activities are regulated by the pacc transcription factor in aspergillus nidulans, we functionally characterized a pacc gene homolog, pac1, from s. sclerotiorum. mutants with l ... | 2003 | 12971602 |
| cdna cloning, expression and characterization of an allergenic l3 ribosomal protein of aspergillus fumigatus. | aspergillus fumigatus (afu) is an important fungal pathogen causing allergic and invasive respiratory disorders. a plethora of multi-functional allergens/antigens secreted by afu have been implicated in pathogenesis. the present study was undertaken to identify and characterize novel afu allergen/antigen by cdna library approach. cdna library of afu was immunoscreened with pooled sera of allergic bronchopulmonary aspergillosis (abpa) patients. the cdna clone, ts1, reacting significantly with spe ... | 2003 | 12974759 |
| the genetics of aspergillus nidulans. | | 1953 | 13040135 |
| nidulin and ustin; two chlorine-containing metabolic products of aspergillus nidulans. | | 1953 | 13087222 |
| [studies on experimental pathogenic power of aspergillus nidulans]. | | 1953 | 13111653 |
| the component fatty acids of the fat of aspergillus nidulans. | | 1955 | 13260180 |
| changes in the composition of the fat of aspergillus nidulans with age of the culture. | | 1956 | 13293185 |
| pathways of cysteine synthesis in aspergillus nidulans. | | 1956 | 13357712 |
| acriflavine-resistant mutants of aspergillus nidulans. | | 1957 | 13439150 |
| induction of cytoplasmic mutations in aspergillus nidulans. | | 1957 | 13440952 |
| studies in the biochemistry of micro-organisms. 101. the colouring matters of species in the aspergillus nidulans group. 2. further observations on the structure of asperthecin. | | 1957 | 13445677 |
| an 8-chromosome map of aspergillus nidulans. | | 1958 | 13520441 |
| [morphology of aspergillus nidulans variants produced by ultraviolet irradiation]. | | 1958 | 13540947 |
| an instance of cytoplasmic inheritance in aspergillus nidulans. | | 1958 | 13542631 |
| abnormal tetrads in aspergillus nidulans. | | 1958 | 13542643 |
| [some features of development of aspergillus nidulans variants produced by ultraviolet irradiation]. | | 1958 | 13551946 |
| an analysis of interference in aspergillus nidulans. | | 1958 | 13554433 |
| nucleo-cytoplasmic interactions in aspergillus nidulans. | | 1958 | 13635551 |
| [resistance of conidia of aspergillus nidulans and aspergillus niger to ultraviolet rays]. | | 1959 | 13643759 |
| a replica plating technique for the isolation of nutritionally exacting mutants of a filamentous fungus (aspergillus nidulans). | | 1959 | 13664901 |
| [accumulation of biomass in greatly altered variants of aspergillus nidulans in various conditions of cultivation]. | | 1959 | 13674056 |
| guido pontecorvo, 29 november 1907 - 25 september 1999. | guido pontecorvo was elected a fellow of the royal society in 1955 for his contributions to the genetics of drosophilia and the fungus aspergillus nidulans. pontecorvo was a leading british geneticist, prominent in the decade preceding the discovery of dna, who enriched our understanding of genes and whose pioneering work on the parasexual cycle in fungi found application in human somatic cell genetics. known to friends as ponte, he had a strong personality. somewhat irascible but warm, with a w ... | 2002 | 13678073 |
| [the genetic relation of the association of aspergillus nidulans, micrococci and substrate]. | | 1961 | 13695539 |
| [on the mechanism of mitotic segregation in aspergillus nidulans]. | | 1960 | 13701538 |
| [morphology of amino-deficient variants of aspergillus nidulans, related to the composition of the media]. | | 1960 | 13751638 |
| [activity of certain oxidative enzymes in a variant of aspergillus nidulans obtained with the aid of ultraviolet rays]. | | 1960 | 13763179 |
| [on activity of hydrolytic enzymes in the aspergillus nidulans variant produced with the aid of ultraviolet rays]. | | 1960 | 13763180 |
| [on the respiration of greatly modified variants of aspergillus nidulans received as a result of the action of ultraviolet rays]. | | 1960 | 13841739 |
| the "red" cytoplasmic variant of aspergillus nidulans. | | 1962 | 13862319 |
| conversion of alpha-aminoadipic acid to l-pipecolic acid by aspergillus nidulans. | | 1962 | 13862932 |
| radiation-induced chromosomal aberrations and lethals in aspergillus nidulans. | the frequency of translocations induced in diploid conidia of aspergillus nidulans by gamma rays was much higher than that of recessive mutants involving a single chromosome. more than half of the surviving nuclei contained viable translocations at radiation doses within the range normally employed for induction of mutants in microorganisms. | 1962 | 13920059 |
| synthesis from sulphate and accumulation of s-sulphocysteine by a mutant strain of aspergillus nidulans. | | 1963 | 13937435 |
| heterokaryon compatibility of unrelated strains in the aspergillus nidulans group. | | 1963 | 13950547 |
| translocations in stock strains of aspergillus nidulans. | | 1962 | 13958240 |
| the genetic analysis of carbohydrate utilization in aspergillus nidulans. | | 1963 | 13974284 |
| the adaptive metabolism of d-galactose in aspergillus nidulans. | | 1963 | 13974285 |
| a sulfite reductase from aspergillus nidulans. | | 1961 | 14009264 |
| independently segregating genetic loci concerned with nitrate reductase activity in aspergillus nidulans. | | 1963 | 14023503 |
| radiation effects and mitotic recombination in diploids of aspergillus nidulans. | | 1963 | 14030092 |
| translocations in stock strains of aspergillus nidulans. | | 1962 | 14030093 |
| heterokaryon compatibility of closely related wild isolates of aspergillus nidulans. | | 1963 | 14080689 |
| the genetical basis of heterokaryon incompatibility in aspergillus nidulans. | | 1963 | 14080690 |
| studies on aspergilli. viii. sulphur requirements of some ascosporic members of the aspergillus nidulans group. | | 1963 | 14086287 |
| effects of disintegration of incorporated 32p in aspergillus nidulans. | | 1963 | 14102201 |
| [apropos of a case of bronchial aspergilloma due to aspergillus nidulans]. | | 1963 | 14111509 |
| the "minute" cytoplasmic variant of aspergillus nidulans. | | 1964 | 14127547 |
| nucleo-cytoplasmic interactions in the 'red' cytoplasmic variant of aspergillus nidulans. | | 1964 | 14127548 |
| uv-induced mitotic recombination in the paba-1 region af aspergillus nidulans. | | 1964 | 14170380 |
| physiological and genetic effects of 8-azaguanine on aspergillus nidulans. | | 1964 | 14202372 |
| aspergillosis (aspergillus nidulans) involving bone. | | 1965 | 14263488 |
| genetic analysis of the phosphatases in aspergillus nidulans. | | 1965 | 14301537 |
| a comparison of the ultraviolet effect on the mitotic recombination in two different cistrons of aspergillus nidulans. | | 1965 | 14333965 |
| studies in the biochemistry of micro-organisms. 94. the colouring matters of species in the aspergillus nidulans group. i. asperthecin, a crystalline colouring matter of aspergillus quadrilineatus thom & raper. | | 1955 | 14363122 |
| [biochemical mutants of aspergillus nidulans obtained after ultraviolet irradiation]. | | 1959 | 14404630 |
| [production of yeast mutants with the aid of ultraviolet rays. ii. morphological characteristics of variants of aspergillus nidulans produced under the influence of various doses of ultraviolet rays]. | | 1959 | 14413024 |
| the processes of spontaneous recombination in vegetative nuclei of aspergillus nidulans. | | 1961 | 14453038 |
| new mycelial mutants in aspergillus nidulans. i. | | 1962 | 14461106 |
| radiation action on the mitotic crossing-over in aspergillus nidulans. ii. | | 1962 | 14461107 |
| an improved selective medium for the formation of ascospores by aspergillus nidulans. | | 1962 | 14463241 |
| accumulation of s-sulphocysteine by a mutant strain of aspergillus nidulans. | | 1962 | 14478165 |
| biochemical genetical studies on the pathway of sulphate assimilation in aspergillus nidulans. | | 1962 | 14478170 |
| genetics and biochemistry of riboflavin auxotrophs of aspergillus nidulans. | | 1962 | 14489746 |
| multiple gata sites: protein binding and physiological relevance for the regulation of the proline transporter gene of aspergillus nidulans. | in aspergillus nidulans, proline can serve both as a carbon and a nitrogen source. the transcription of the prnb gene, encoding the proline transporter, is efficiently repressed only by the simultaneous presence of ammonium and glucose. thus, repression of this gene demands the activation of the crea repressor and the inactivation of the positive-acting gata factor area. repression of all other prn structural genes results largely from inducer exclusion. in an area null mutation background, prnb ... | 2003 | 14507380 |
| 4'-phosphopantetheinyl transferase-encoding npga is essential for siderophore biosynthesis in aspergillus nidulans. | aspergillus nidulans produces two major siderophores: it excretes triacetylfusarinine c to capture iron and contains ferricrocin as an intracellular iron-storage compound. siderophore biosynthesis involves the enzymatic activity of nonribosomal peptide synthetases (nrps). nrps contain 4'-phosphopantetheine as an essential prosthetic group, which is attached by 4'-phosphopantetheinyl transferases. a. nidulans appears to possess at least one gene, npga, encoding such an enzyme. using a strain carr ... | 2003 | 14508603 |
| discrimination between gene expression patterns in the invasive margin and the tumour core of malignant melanomas. | genes that determine the invasive capacity of the invasive front of malignant melanomas (mm) have not yet been systematically investigated in vivo. therefore, we combined laser pressure catapulting (lpc) microdissection with cdna microarray technology (dermarray, research genetics, representing about 5700 genes) to systematically analyse differences in gene expression profiles between the invasive margin and the tumour centre in nine cases of vertical growth phase mm. signal-to-noise statistical ... | 2003 | 14512792 |
| the aspergillus nidulans alca promoter drives tightly regulated conditional gene expression in aspergillus fumigatus permitting validation of essential genes in this human pathogen. | aspergillus fumigatus causes invasive aspergillosis, a mycosis that is usually fatal in immunocompromised patients. functional genomics in this fungus will aid the discovery of novel antifungal drugs to treat invasive aspergillosis. however, there is still a need for appropriate molecular genetic tools to facilitate such functional studies. here, we describe the use of a conditional gene expression system allowing the identification of novel therapeutic targets through validation of essential ge ... | 2003 | 14516763 |
| diversity of polyketide synthase gene sequences in aspergillus species. | fungal polyketide synthases are responsible for the biosynthesis of several mycotoxins and other secondary metabolites. the aim of our work was to investigate the diversity of polyketide synthases in aspergillus species using two approaches: pcr amplification using oligonucleotide primers, and bioinformatics. ketosynthase domain probes amplified dna fragments of about 700 bp in each examined isolate. sequences of these domains were aligned and analyzed by phylogenetic methods. the ketosynthase d ... | 2003 | 14527661 |
| the kinesin family member bimc contains a second microtubule binding region attached to the n terminus of the motor domain. | the kinesin family member bimc has a highly positively charged domain of approximately 70 amino acids at the n terminus of the motor domain. motor domain constructs of bimc were prepared with and without this extra domain to determine its influence. the level of microtubules needed for half saturation of the atpase of bimc motor domain constructs is reduced by approximately 7000-fold at low ionic strength upon addition of this extra n-terminal extension. although the change in microtubule affini ... | 2003 | 14530265 |
| a novel extracellular multicopper oxidase from phanerochaete chrysosporium with ferroxidase activity. | lignin degradation by the white rot basidiomycete phanerochaete chrysosporium involves various extracellular oxidative enzymes, including lignin peroxidase, manganese peroxidase, and a peroxide-generating enzyme, glyoxal oxidase. recent studies have suggested that laccases also may be produced by this fungus, but these conclusions have been controversial. we identified four sequences related to laccases and ferroxidases (fet3) in a search of the publicly available p. chrysosporium database. one ... | 2003 | 14532088 |
| viability of dried vegetative cells or filaments, survivability and/or reproduction under water and light stress, and following heat and uv exposure in some blue-green and green algae. | vegetative cells in dried, mucilagenous mass of gloeocapsa aeruginosa and aphanothece nidulans, reticulum of hydrodictyon reticulatum, mucilagenous mass of chroococcus minor, and filaments of oedogonium sp. and scytonema hofmanni died within 1/2, 1/2, 1/2, 1, 3 and 6 h, respectively, while dried vegetative filaments of phormidium foveolarum retained under similar storage conditions viability for 4 d. p. foveolarum tolerated 1 mol/l nacl. the resistance to desiccation in p. foveolarum exhibited s ... | 2003 | 14533482 |
| cloning and expression analysis of the atp-binding cassette transporter gene mfabc1 and the alternative oxidase gene mfaox1 from monilinia fructicola. | brown rot, caused by moniliniafructicola (g wint) honey, is a serious disease of peach in all commercial peach production areas in the usa, including south carolina where it has been primarily controlled by pre-harvest application of 14-alpha demethylation (dmi) fungicides for more than 15 years. recently, the qo fungicide azoxystrobin was registered for brown rot control and is currently being investigated for its potential as a dmi fungicide rotation partner because of its different mode of ac ... | 2003 | 14561072 |
| the serine/threonine kinase nek6 is required for cell cycle progression through mitosis. | the aspergillus nidulans protein nima (never in mitosis, gene a) is a protein kinase required for the initiation of mitosis, whereas its inactivation is necessary for mitotic exit. here, we demonstrate that human nima-related kinase 6 (nek6) is required for mitotic progression of human cells. nek6 is phosphorylated and activated during m phase. inhibition of nek6 function by either overexpression of an inactive nek6 mutant or elimination of endogenous nek6 by sirna arrests cells in m phase and t ... | 2003 | 14563848 |
| genetic transformation of monascus purpureus dsm1379. | monascus purpureus was transformed into hygromycin b resistance with hygromycin b phosphotransferase (hph) fused to aspergillus nidulans trpc or a putative monascus purpureus gpd1 promoter by electroporation. among five strains, only m. purpureus dsm1397 was a competent recipient. normal growth and sporulation on media containing up to 500 mg hygromycin b l(-1) occurred up to five generations. upon transformation of the strain with the green fluorescent protein gene (sgfp) as a model gene and hp ... | 2003 | 14571974 |
| the aspergillus nidulans swoc1 mutant shows defects in growth and development. | previous work identified swoc1 as a single-gene mutant with defects in polarity establishment. in this study swoc1 was shown to have defects in endocytosis, compartmentation, nuclear distribution, and conidiation. temperature-shift experiments showed that the swoc1 mutant establishes multiple random sites of germ tube emergence. surprisingly, these experiments also showed that even a slight delay in polarity establishment causes defects in later vegetative growth and asexual reproduction. the sw ... | 2003 | 14573468 |
| mutational analysis of the major proline transporter (prnb) of aspergillus nidulans. | prnb, the l-proline transporter of aspergillus nidulans, belongs to the amino acid polyamine organocation (apc) transporter family conserved in prokaryotes and eukaryotes. in silico analysis and limited biochemical evidence suggest that apc transporters comprise 12 transmembrane segments (tms) connected with relatively short hydrophilic loops (l). however, very little is known on the structure-function relationships in apc transporters. this work makes use of the a. nidulans prnb transporter to ... | 2003 | 14578044 |
| the azga purine transporter of aspergillus nidulans. characterization of a protein belonging to a new phylogenetic cluster. | the azga gene of aspergillus nidulans encodes a hypoxanthine-adenine-guanine transporter. it has been cloned by a novel transposon methodology. the null phenotype of azga was defined by a number of mutations, including a large deletion. in mycelia, the azga gene is, like other genes of purine catabolism, induced by uric acid and repressed by ammonium. its transcription depends on the pathway-specific uay zinc binuclear cluster protein and the broad domain area gata factor. azga is not closely re ... | 2004 | 14597637 |
| characterization of phia, a gene essential for phialide development in aspergillus nidulans. | we have previously identified genes and proteins involved in the fungal response to the streptomyces-produced antibiotics, bafilomycin b1 and concanamycin a, known inhibitors of v-atpases. using mrna differential display we identified an aspergillus nidulans gene with 30-fold up-regulated expression in the presence of bafilomycin. this gene, here denoted phia, and its gene product, were further characterized by targeted gene disruption and immunohistochemistry. phenotypically, the phia mutation ... | 2003 | 14599891 |
| addendum to "the ph-induced glycosylation of secreted phosphatases is mediated in aspergillus nidulans by the regulatory gene pacc-dependent pathway" [fungal genet. biol. 39 (2003) 286-295]. | | 2003 | 14599896 |
| intron requirement for afp gene expression in trichoderma viride. | the 430 bp orf of the aspergillus giganteus antifungal protein (afp) gene, containing two small introns, was fused between the promoter and the terminator of the aspergillus nidulans trpc gene. the afp gene in this vector produced detectable levels of spliced mrna in trichoderma viride. in contrast, in the same vector configuration, its 285 bp intronless derivative showed no accumulation of mrna when transformed into t. viride. such expression results were confirmed at the protein level. this fa ... | 2003 | 14600221 |
| a novel improved method for aspergillus nidulans transformation. | we systematically investigated the efficiency of aspergillus nidulans transformation using protoplasts prepared from different stages of conidiospore germination and young mycelium. using standard integrative plasmids, increased transformation yields were obtained with protoplasts isolated from a specific stage coincident with germ tube emergence. this increase ranged, on the average, from two- to eightfold depending on different plasmids used. transformation efficiencies with a replicative plas ... | 2003 | 14607411 |
| fungal metabolic model for 3-methylcrotonyl-coa carboxylase deficiency. | aspergillus nidulans is able to use leu as the sole carbon source through a metabolic pathway leading to acetyl-coa and acetoacetate that is homologous to that used by humans. mcca and mccb, the genes encoding the subunits of 3-methylcrotonyl-coa carboxylase, are clustered with ivda encoding isovaleryl-coa dehydrogenase, a third gene of the leu catabolic pathway, on the left arm of chromosome iii. their transcription is induced by leu and other hydrophobic amino acids and repressed by glucose. p ... | 2004 | 14612443 |
| nitrogen metabolism and virulence of candida albicans require the gata-type transcriptional activator encoded by gat1. | nitrogen acquisition and metabolism is central to microbial growth. a conserved family of zinc-finger containing transcriptional regulators known as gata-factors ensures efficient utilization of available nitrogen sources by fungi. gata factors activate expression of nitrogen catabolic pathways when preferred nitrogen sources are absent or limiting, a phenomenon known as nitrogen catabolite repression. gat1 of candida albicans encodes a gata-factor homologous to the area protein of aspergillus n ... | 2003 | 14617156 |
| reactive oxygen species generated by microbial nadph oxidase noxa regulate sexual development in aspergillus nidulans. | nadph oxidases (nox) have been characterized as higher eukaryotic enzymes used deliberately to produce reactive oxygen species (ros). the recent discovery of new functional members of the nox family in plants and animals has led to the recognition of the increasing importance of ros as signals involved in regulation of diverse cellular processes such as defence, growth and signalling. here, we address the role of nadph oxidase-generated ros in the biology of the filamentous fungus aspergillus ni ... | 2003 | 14622412 |
| the alternative d-galactose degrading pathway of aspergillus nidulans proceeds via l-sorbose. | the catabolism of d-galactose in yeast depends on the enzymes of the leloir pathway. in contrast, aspergillus nidulans mutants in galactokinase ( gale) can still grow on d-galactose in the presence of ammonium-but not nitrate-ions as nitrogen source. a. nidulans gale mutants transiently accumulate high (400 mm) intracellular concentrations of galactitol, indicating that the alternative d-galactose degrading pathway may proceed via this intermediate. the enzyme degrading galactitol was identified ... | 2004 | 14624333 |
| overlap of nuclear localisation signal and specific dna-binding residues within the zinc finger domain of pacc. | the transcription factor pacc, mediating regulation of gene expression by ambient ph in the genetically amenable fungus aspergillus nidulans, contains a three zinc finger dna-binding domain (zf-dbd) including a nuclear localisation signal (nls). we selected 38 novel mutations impairing pacc function, of which 21 missense mutations identify individual residues essential for zf-dbd structure/function. our functional analysis agrees with our previous conclusion that finger 1 does not bind dna and p ... | 2003 | 14636595 |