| surfactant protein d. increased accumulation in silica-induced pulmonary lipoproteinosis. | surfactant protein d (sp-d) (cp4) is a collagenous surfactant-associated carbohydrate binding protein that is synthesized and secreted by alveolar epithelial cells. previous studies have shown that intratracheal administration of crystalline silica to rats elicits a marked increase in the alveolar accumulation of surfactant lipids and surfactant protein a (sp-a). the authors examined the accumulation of sp-d using this animal model of alveolar proteinosis. immunoperoxidase localization of sp-d s ... | 1991 | 1656758 |
| lupanine hydroxylase, a quinocytochrome c from an alkaloid-degrading pseudomonas sp. | lupanine 17-hydroxylase, the first enzyme in the pathway for bacterial degradation of the alkaloid, lupanine, was purified from a pseudomonas sp. the enzyme acts by initial dehydrogenation of the substrate, and cytochrome c was used as electron acceptor in assays. it had an mr of 66,000 by ultracentrifuge studies and 74,000 by gel filtration. the visible absorption spectrum was that of a cytochrome c, and a stoicheiometry of one haem group per molecule of enzyme was calculated. sds/page gave a s ... | 1991 | 1656935 |
| roles of collagenous domain and oligosaccharide moiety of pulmonary surfactant protein a in interactions with phospholipids. | pulmonary surfactant protein a (sp-a), a main component of lung-specific lipid-protein complex (pulmonary surfactant), is characterized by a collagen-like sequence in its amino terminal half and by n-linked glycosylation. the structural characteristics necessary for the various functions of sp-a are not yet completely understood. in the present study we examined the roles of the oligosaccharide moiety of sp-a and its collagenous domain in causing the aggregation of phospholipid liposomes and enh ... | 1991 | 1656980 |
| vagus nerve and spinal cord projecting neurons demonstrated by horseradish peroxidase and different fluorescent dyes. | vagal projecting (vp) neurons were localized by intraneural injections of fluorescent dyes or cholera toxin conjugated horseradish peroxidase (ct-hrp) or by intraperitoneal injection of fluorescent dyes. spinal projecting (sp) neurons were localized by injecting ct-hrp or contrasting dyes into the c4/c5 cord segments. no doubly labelled neurons were seen in the three nuclei known to project to both vagus nerve and spinal cord, viz., dorsal nucleus of the vagus (dnv), nucleus ambiguous complex (n ... | 1991 | 1657809 |
| unique modification of adenine in genomic dna of the marine cyanobacterium trichodesmium sp. strain nibb 1067. | the genomic dna of the marine nonheterocystous nitrogen-fixing cyanobacterium trichodesmium sp. strain nibb 1067 was found to be highly resistant to dna restriction endonucleases. the dna was digested extensively by the restriction enzyme dpni, which requires adenine methylation for activity. the dna composition, determined by high-performance liquid chromatography (hplc), was found to be 69% at. surprisingly, it was found that a modified adenine which was not methylated at the usual n6 position ... | 1991 | 1657876 |
| identification of a novel composite transposable element, tn5280, carrying chlorobenzene dioxygenase genes of pseudomonas sp. strain p51. | analysis of one of the regions of catabolic plasmid pp51 which encode chlorobenzene metabolism of pseudomonas sp. strain p51 revealed that the tcba and tcbb genes for chlorobenzene dioxygenase and dehydrogenase are located on a transposable element, tn5280. tn5280 showed the features of a composite bacterial transposon with iso-insertion elements (is1066 and is1067) at each end of the transposon oriented in an inverted position. when a 12-kb hindiii fragment of pp51 containing tn5280 was cloned ... | 1991 | 1657878 |
| presence of various carbohydrate moieties including beta-galactose and n-acetylglucosamine residues on solubilized porcine brain neurokinin-1/substance p receptors. | neurokinin-1 (nk-1)/substance p (sp) receptors were solubilized using 10 mm 3-[( cholamidopropyl)-dimethylammonio]-1- propanesulfate from porcine striatal membranes (solubilization yield, 80%). in solubilized preparations, [3h]sp apparently bound to a single class of high-affinity sites (kd = 0.82 +/- 0.13 nm) as in membrane homogenates. the ligand selectivity pattern observed in both membrane and solubilized receptor preparations indicated that [sar9,met(o2)11]sp = sp much greater than senktide ... | 1991 | 1658228 |
| [comparative multicenter study of bovine insulin with forms more purified of swine and human insulin in the treatment of type 1 diabetes mellitus]. | a multicentric double-blind randomized study was organized to investigate the relationship between insulin antibodies and metabolic control in type i diabetics who changed from bovine insulin to monopic porcine and monocomponent human insulin. twenty eight type i diabetic patients treated with bovine insulin (proinsulin less than 3,000 ppm) were selected. in a 6 month study, 9 patients maintained bovine insulin, 9 changed to monopic porcine insulin (proinsulin less than 10 ppm) and 10 to human i ... | 1991 | 1658875 |
| [prevalence of cryptosporidiosis in diarrheic syndrome in hiv positive patients]. | during the period february 1987-june 1988, we examined 542 stool samples of 271 hiv positive patients both with and without full-blown aids. one hundred patients with either acute or chronic diarrhea and 180 patients without diarrhea were studied. the stool samples were examined for the presence of cryptosporidium sp., other protozoa, helmints, and pathogenic enterobacteria. we found a prevalence of 14.3% of cryptosporidium sp. in patients with full-blown disease and diarrhea. we encountered no ... | 1991 | 1658876 |
| differential expression of na channels in functional subpopulations of rat lactotropes. | we have investigated the voltage-dependent na channel activity of single lactotropes in pituitary cultures from adult male rats by recording whole cell na+ currents under voltage clamp. cells were identified by their ability to secrete prolactin in the basal state as measured with the reverse hemolytic plaque assay. we found that cells with elevated secretory rates [large-plaque (lp) lactotropes] present relatively large na+ currents in response to depolarization, whereas na+ currents in cells s ... | 1991 | 1659213 |
| characterization of sp-5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole- 3',5'-monophosphorothioate (sp-5,6-dcl-cbimps) as a potent and specific activator of cyclic-amp-dependent protein kinase in cell extracts and intact cells. | a newly designed cyclic amp (camp) analogue, sp-5,6-dichloro-1-beta-d- ribofuranosylbenzimidazole-3',5'-monophosphorothioate (sp-5,6-dcl-cbimps), and 8-(p-chlorophenylthio)-camp (8-pcpt-camp) were compared with respect to their chemical and biological properties in order to assess their potential as activators of the camp-dependent protein kinases (camp-pk) in intact cells. sp-5,6-dcl-cbimps was shown to be both a potent and specific activator of purified camp-pk and of camp-pk in platelet membr ... | 1991 | 1659381 |
| [method and procedures in bacteriological study of necrotic teeth]. | research was conducted of 160 radicular canals with necrotic pulp. results of different bacteriological analyses are presented. culture analyses in aerobic and anaerobic media, resulted in the isolation of staphylococcus epidermidis, streptococcus viridans and corynebacterium sp in the group studied, as the most frequent bacteria. there was no evidence of a specific germ linked with the pulp necrosis. | 1991 | 1659855 |
| assignment of a human serum glycoprotein sp-40,40 gene (cli) to chromosome 8. | sp-40,40 is a serum glycoprotein consisting of two different subunits (alpha and beta) assembled into a dimer by disulfide bonds. northern blot hybridization, using total rna from several cell lines, showed that sp-40,40 is expressed in glioblastoma and testicular tumor cells, as well as hepatoma cells. spot blot hybridization of flow-sorted human chromosomes, using a sp-40,40 cdna fragment as a probe, localized the gene for sp-40,40 to human chromosome 8. this gene has been given the designatio ... | 1991 | 1660393 |
| organization and characterization of three genes involved in d-xylose catabolism in lactobacillus pentosus. | a cluster of three genes involved in d-xylose catabolism (viz. xylose genes) in lactobacillus pentosus has been cloned in escherichia coli and characterized by nucleotide sequence analysis. the deduced gene products show considerable sequence similarity to a repressor protein involved in the regulation of expression of xylose genes in bacillus subtilis (58%), to e. coli and b. subtilis d-xylose isomerase (68% and 77%, respectively), and to e. coli d-xylulose kinase (58%). the cloned genes repres ... | 1991 | 1660563 |
| conjugational transfer of recombinant dna in cultures and in soils: host range of pseudomonas putida tol plasmids. | recombinant tol plasmid pwwo-eb62 allows pseudomonas putida to grow on p-ethylbenzoate. this plasmid can be transferred to other microorganisms, and its catabolic functions for the metabolism of alkylbenzoates are expressed in a limited number of gram-negative bacteria, including members of pseudomonad rrna group i and escherichia coli. transfer of the recombinant plasmid to erwinia chrysanthemi was observed, but transconjugants failed to grow on alkylbenzoates because they lost catabolic functi ... | 1991 | 1660698 |
| amyloid beta protein substituent peptides do not interact with the substance p receptor expressed in cultured cells. | the amyloid beta protein (abp) has been shown to interact with the substance p (sp) receptor in a cell culture model that may mimic the pathogenesis of alzheimer's disease. in the present study, however, 4 fragments of abp (beta 1-42, beta 1-16, beta 17-28, and beta 25-35) failed to interact with sp-induced ca2+ mobilization in sp receptor-expressing cultured cells. therefore, the action of these abp-related peptides in our cultured cells is unrelated to the sp receptor. | 1991 | 1661816 |
| paf receptor antagonists influence asphyxia-induced changes of the inner ear. | the influence of a transient asphyxia on cochlear potentials, i.e. endolymphatic potential (ep), summating potential (sp) and cochlear microphonics (cm) was investigated in guinea pigs which were injected with two different paf receptor antagonists before. it could be shown that apafant (web 2086) and bepafant (web 2170) significantly reduced the asphyxia-induced potential changes compared to controls. the results suggest a mediating role of paf in the asphyxia model. the impact of the findings ... | 1991 | 1662548 |
| characterization of the epoxide hydrolase from an epichlorohydrin-degrading pseudomonas sp. | an epoxide hydrolase was purified to homogeneity from the epichlorohydrin-utilizing bacterium pseudomonas sp. strain ad1. the enzyme was found to be a monomeric protein with a molecular mass of 35 kda. with epichlorohydrin as the substrate, the enzyme followed michaelis-menten kinetics with a km value of 0.3 mm and a vmax of 34 mumol.min-1.mg protein-1. the epoxide hydrolase catalyzed the hydrolysis of several epoxides, including epichlorohydrin, epibromohydrin, epoxyoctane and styrene epoxide. ... | 1991 | 1662605 |
| effect of sodium chloride on transport of bacteria in a saturated aquifer material. | determinations were made of the influence of nacl concentration, cell density, and flow velocity on the transport of pseudomonas sp. strain kl2 through columns of aquifer sand under saturated conditions. a pulse-type boundary condition was used. the experiments were conducted by using 0.3-m-long plexiglas columns with an internal diameter of 0.05 m. when a 1-h pulse of a 0.01 m nacl solution containing 10(8) cells per ml was added at a flow rate of 10(-4) m s-1, the bacterial density in the effl ... | 1991 | 1662934 |
| magnitude of the negative summating potential varies with perilymph calcium levels. | previous results demonstrated that nimodipine, an l-type of ca2+ channel antagonist, abolished the negative summating potential (sp) recorded from anesthetized guinea pigs (bobbin et al., 1990), suggesting that ca2+ is involved in generation of the negative sp. therefore we examined the effect of changing concentrations of perilymph ca2+ on this cochlear potential. perilymph spaces of guinea pig cochleae were perfused with artificial perilymph solutions containing zero mm ca2+, zero mm ca2+ with ... | 1991 | 1663103 |
| [in-vitro and in-vivo activity of itraconazole]. | itraconazole is a lipophilic triazole with potent in vitro activity. it is also highly efficacious after topical, oral and parenteral administration. the antifungal activity of itraconazole has been evaluated against more than 6500 different strains, belonging to more than 260 fungal species using the serial decimal dilution test in fluid media (bhi broth). candida, torulopsis, cryptococcus, pityrosporum (dixon broth), various other yeasts, dermatophytes, aspergillus, penicillium, sporothrix, di ... | 1991 | 1663204 |
| infection-associated hemophagocytic syndrome. evidence for epstein-barr virus gene expression. | autopsy specimens from a patient with infection-associated hemophagocytic syndrome (iahs) were evaluated for the presence of epstein-barr virus dna and rna using in situ hybridization. frozen sections of liver, lymph node, and spleen were probed with ebv bam hi-h & w, gamma interferon, and sp-65 plasmid dna as a negative control probe. hybridization patterns before and after treatment with ribonuclease a were examined. both ebv probes produced diffuse hybridization throughout the tissues; in add ... | 1991 | 1664662 |
| modulation of the immune response by tachykinins. | neuro-immunology is becoming an increasingly important discipline of immunology. this review has examined the immunomodulatory function of one group of neuropeptides, the tk, particularly sp and nka. these peptides are localized in primary afferent nerves which have been shown to innervate several immune organs. in addition, binding sites for the tk have been demonstrated in thymus, spleen and lymph node. several immune cell types also express neurokinin receptors including human circulating lym ... | 1991 | 1664817 |
| expression of synaptophysin during synapse formation between dissociated cortical neurons. | cerebral cortical cells from fetal rats (14 days) were cultured in vitro (7-104 days). localization of synaptophysin was examined immunocytochemically using a monoclonal antibody (sy-38) against synaptophysin. synaptophysin-immunoreactive (sp-ir) spots were distributed on the surface of the cells. electron-microscopic observation showed that the sp-ir staining was localized at presynaptic terminals, indicating that the sp-ir spots observed will, fluorescence microscopy represent presynaptic term ... | 1991 | 1664927 |
| paf and ltb4 biosynthesis in the human neutrophil: effects of putative inhibitors of phospholipase a2 and specific inhibitors of 5-lipoxygenase. | the effect of several putative phospholipase a2 (pla2) inhibitors on [3h]-acetate incorporation into platelet-activating factor (paf) upon calcium ionophore a23187 stimulation of purified human neutrophils (pmn) were evaluated in vitro. pla2 inhibitors such as p-bromophenacyl bromide (pbpb), ellagic acid, aristolochic acid and gossypol were without effect or only weakly inhibited paf biosynthesis. luffariellolide, a potent pla2 inhibitor isolated from the marine sponge luffariella sp., dose-depe ... | 1991 | 1665306 |
| the effect of tubero-infundibular dopaminergic neurons on prolactin and alpha-melanocyte stimulating hormone release. | the effect of tubero-infundibular dopaminergic neurons (tida) on the release of prolactin (prl) and alpha-melanocyte stimulating hormone (alpha-msh) was studied in median eminence-lesioned (mel) male rats (n = 6-28). plasma prl and alpha-msh levels were significantly elevated 2 (86.1 +/- 19.8 and 505.1 +/- 19.1 ng/ml), 4 (278.7 +/- 15.5 and 487.4 +/- 125.1 ng/ml), 7 (116.2 +/- 16.2 and 495.8 +/- 62.6 ng/ml) and 14 (247.3 +/- 26.1 and 448.4 +/- 63.8 ng/ml) days after mel when compared to sham-ope ... | 1991 | 1665725 |
| neuropeptide gamma, the most potent contractile tachykinin in human isolated bronchus, acts via a 'non-classical' nk2 receptor. | cumulative contractile response curves to neurokinin a (nka) and neuropeptide gamma (np gamma) were obtained in human isolated bronchus, in the presence of phosphoramidon 10 microm. np gamma was approximately 10-fold more potent than nka (pd2 values 8.6 +/- 0.4 and 7.3 +/- 0.3 respectively, n = 6; p less than 0.01). the nk1-selective agonist [sar9, met(o2)11]-sp and the nk3 selective agonist senktide produced negligible contraction. response curves to np gamma and nka were unaffected by the nk2 ... | 1991 | 1665897 |
| isolation and structure determination of novel phosphatidylinositol kinase inhibitors, echiguanines a and b, from streptomyces sp. | in the course of a screening program for phosphatidylinositol kinase inhibitors, we discovered novel inhibitors, echiguanines a and b, from the culture broth of a streptomyces strain mi698-50f1 (fermp-11563). the structures of echiguanines a and b were revealed to be n-(2-amidinoethyl)-2-amino-4-hydroxy-7h-pyrrolo[2,3- d]pyrimidine-5-carboxamide and n-(3-aminopropyl)-2-amino-4-hydroxy-7h-pyrrolo[2,3-d]pyrimidine- 5-carboxamide, respectively, by nmr spectroscopy and x-ray crystallography. echigua ... | 1991 | 1666120 |
| effects of some neurokinin a analogues on tachykinin-induced contraction of guinea pig trachea. | a series of analogues of neurokinin a (nka) has been synthesized and characterized by testing for their abilities, in vitro, to contract guinea pig tracheal smooth muscle or to antagonize nka-, nkb- and sp-induced contraction of this tissue. substitution of nka residues gly8 or leu9 by conformationally restricting amino acids produced peptides that were antagonists of nka action, but the type and specificity of the antagonism depended on the size of the peptide. thus, while [ala5, aib8, leu10]nk ... | 1991 | 1666182 |
| binding sites for tachykinin peptides in the brain and stomach of the dogfish, scyliorhinus canicula. | in membranes of dogfish brain and stomach, two binding sites for tachykinins were identified. one site specifically bound [125i]-bolton-hunter substance p (bh-sp) and the rank potency of tachykinins to compete for bh-sp binding revealed similarities with the rank potency of an nk1 receptor. the pharmacology of the other site, which specifically bound [125i]-bolton-hunter scyliorhinin ii (bh-scy ii), did not resemble any of the mammalian tachykinin receptors. the rank potency to inhibit bh-scy ii ... | 1991 | 1666186 |
| studies of bacterial, rotaviral and cryptosporidium etiology of acute diarrheal diseases in hospitalized children. | 657 hospitalized children with acute diarrheal disease were studied for bacterial and rotaviral etiology. cryptosporidium presence was followed in 123 children. intestinal pathogens were detected in 195 (29.6%) cases: 132 (20.3%) enterobacteria, 47 (7.1%) rotaviruses, 4 (3.2%) cryptosporidium sp. and 12 (1.8%) combined infections. among enterobacteria, e. coli was the most frequent (10% cases) with enteropathogenic (epec), enterotoxigenic (etec), enterohemorrhagic (ehec) and enteroinvasive (eiec ... | 1991 | 1666314 |
| high level expression in escherichia coli of isopenicillin n synthase genes from flavobacterium and streptomyces, and recovery of active enzyme from inclusion bodies. | a t7 promoter-based vector was used to express the isopenicillin n synthase (ipns) genes of flavobacterium sp. 12,154 and streptomyces jumonjinensis in escherichia coli. most of the ipns synthesized at 37 degrees c, and representing some 22% and 51% of the total cell protein respectively, occurred in an insoluble, enzymatically inactive form. active ipns was recovered in a rapid and simple two-step procedure in which the insoluble material was first denatured in 5 m urea and then refolded by pas ... | 1991 | 1666621 |
| developmental changes in ecg associated with heart rate are similar in squirrel monkey and human infants. | interest in refining noninvasive methods of diagnosis and further characterization of squirrel monkeys (saimiri sp.) as a model for pediatric cardiology studies led to this investigation of electrocardiogram (ecg) changes associated with changes in age and position. during a single delivery season, ecgs were performed at 1 day, 1 month, and 1 year of age. for each age group, ecgs were recorded with animals in dorsal, ventral, and right lateral recumbency. the 1-day-old group had the lowest heart ... | 1991 | 1667205 |
| enhancement of the immune response elicited with foot-and-mouth disease virus vaccines by an extract of the mycobacterium sp. wall. | the immunomodulating effect of an extract of the cell wall of mycobacterium sp. (wsf, vetrepharm inc., london, canada) in foot-and-mouth disease virus inactivated vaccines was tested in a murine model. the duration of immunity, protection, stimulation of immunocompetent cells acting on the long-lasting secondary response and possible tissue damage were examined. the incorporation of 10 micrograms wsf into aqueous and oil vaccines induced a high and long-lasting specific antibody response. the ne ... | 1991 | 1667346 |
| preparation, characterization, and antiviral activity of microbial metabolites of stemodin. | screening studies for microbial transformation products of stemodin [2] have identified a number of microbial metabolites. scale-up fermentation with rhizopus arrhizus atcc 11145 and streptomyces sp. nrrl 5691 have resulted in the production of five metabolites that have been characterized with the use of 2d nmr and x-ray techniques. these metabolites have been identified as 18-hydroxystemodin [6], 16,18-dihydroxystemodin [7], 8 beta-hydroxystemodin [8], 8 beta, 18-dihydroxystemodin [9], and 7 b ... | 1991 | 1667410 |
| [cloning and orientation of a promoter of thermophilic thiobacillus sp]. | thiobacillus sp. is an obligate autotrophic thermophilic bacterium which was isolated from an acidic hot spring in yunnan province. its optimum growth temperature is 45-50 degrees c and its optimum ph is 2.0-3.0. using dna recombinant technique, we inserted the hindiii fragments of the thiobacillus sp. chromosomal dna into the hindiii site of promoter-probe plasmid psdsi (aprtcs, 5.65 kb). transformants resistant to tetracycline were obtained on tc plates (12 micrograms/ml). of these, twenty tra ... | 1991 | 1667559 |
| inoculation of infectious pancreatic necrosis virus serotype sp did not cause pancreas disease in atlantic salmon (salmo salar l.). | atlantic salmon were selected from a fish farm with no previous record of pancreas disease (pd) or infectious pancreatic necrosis virus (ipnv) infection. groups of fish were inoculated with either ipnv (strain sp) from cell culture, organ material from fish with pd or control material as phosphate-buffered saline (pbs). virological, histological and immunohistochemical examinations were carried out throughout the experiment. none of the fish died or showed clinical symptoms of pd. histological e ... | 1991 | 1667971 |
| [an electrophysiological study of the brain of rats with different resistances to oxygen deficiency in acute hypoxia]. | the fourier spectre of power (sp) and bioelectrical activity of the cortex, hippocampus and hypothalamus were studied in unrestrained rats with high (hr) and low (lr) resistance to oxygen deficiency before and after their stay at the "altitude" of about 11,000 m. after acute hypobaric hypoxia there were the eeg changes in lr rats: displacement of the sp peak in low-frequency activity and decreasing of absolute power of all the ranges of sp. in one hour after the "altitude", these eeg parameters ... | 1991 | 1668148 |
| efflux of acetylcholine in dimorphic skeletal muscle from castrated male rats. | the effect of testosterone on motor neurons of dimorphic muscles is demonstrable by the increased frequency of miniature end-plate potentials (mepp) and decreased end-plate acetylcholinesterase activity observed in castrated rats. no change occurs in induced acetylcholine (ach) release. although these muscles atrophy after castration there is no loss of muscle fibers. in the present study we reinvestigate the neuromuscular transmission in levator ani (la) muscles from normal (n) adult (120 days) ... | 1991 | 1668374 |
| [the forms of the intercellular contacts of leishmania in culture]. | two kinds of intercellular interactions have been observed in cultures of 12 investigated leishmania species (l. major, l. tropica, l. donovani, l. infantum, l. sp. zma, l. mexicana, l. hertigi, l. braziliensis, l. tarentolae, l. adleri, l. gymnodactyli, l. gulickae). the first kind looks as adhesion of two specimens with their fore-ends. this way is characteristic of promastigotes of different morphotypes as well as of the interphase and dividing organisms to be most frequently seen in l. mexic ... | 1991 | 1668547 |
| behavioural effects of selective tachykinin agonists in midbrain dopamine regions. | the effects of selective nk-1, nk-2 and nk-3 tachykinin agonists in midbrain dopamine cell containing regions were investigated in the rat. the nk-3 agonist senktide induced locomotion, rearing and sniffing following infusion into the substantia nigra pars compacta, and to a lesser extent in the ventral tegmental area. these behavioural responses were not seen following infusion of the selective nk-1 agonist [sar9,met (o2)11]sp or the nk-2 agonist [n1e10]nka4-10. in contrast, grooming was induce ... | 1991 | 1668812 |
| topochemistry of blood cells of the gallus domesticus (aves, galliforme). | blood smears of both male and female chicken gallus domesticus were analysed by using the following topochemical methods: a) periodic acid-schiff (pas) for glycogen. b) mercury-bromophenol blue for protein. c) o-toluidine for myeloperoxidase. d) sudan black b for lipid. the pas reaction revealed glycogen in the cytoplasm of all thrombocytes and in a few heterophils. the presence of proteins was evidenced in all types of cells. however variation in the intensity of staining of protein granules wa ... | 1991 | 1668883 |
| [enzyme activity in leishmania]. | some enzymes produced by the protozoon leishmania can have an important role in the intraphagocytic survival of the parasites. the production of hydrolytic enzymes by promastigotes belonging to two leishmania sp. (major and infantum) and maintained in different experimental conditions was evaluated. the proteolytic activity was moderate, while superior was the production of acid phosphatase in l. infantum. an increase of the trypsin-like activity was observed after incubation of protozoa for 24 ... | 1991 | 1668970 |
| quantification of the potencies of edrf-releasers from isolated rabbit aortic strips. | we have compared several known releasers of endothelium-derived relaxing factor (edrf)(13) in respect to their potencies to generate edrf by endothelium of rabbit aortic strips (rba) superfused with krebs' buffer. the vasorelaxation by edrf which is equivalent to 10 pmoles of gtn was evoked by 0.7 pmoles of substance p(sp), 50 pmoles of acetylcholine (ach), 521 pmoles of calcium ionophore a 23187, 2720 pmoles of adp. threshold potencies of these agonists are inversely proportional to the maximum ... | 1990 | 1669477 |
| 5923-dni, a new deoxyribonuclease inhibitor produced by streptomyces sp. strain no. a-5923. | | 1991 | 1669835 |
| sarcocystis neotomafelis sp. n (protozoa; apicomplexa) from the woodrat neotoma micropus in mexico. | the developmental cycle of a new species of sarcocystis (s. neotomafelis) was investigated. cysts obtained from the muscle fibers of the woodrats were given orally to 19 laboratory newborn kittens, 5 pups and 5 crotalids. infection developed only in newborn kittens. macrogametes, microgamonts and immature oocysts were observed principally in the jejunum. immature oocysts were detected at 6-21 post infection days. based on morphological observations made by light and electron microscopy, and repe ... | 1991 | 1670266 |
| molecular cloning of the genes encoding two chaperone proteins of the cyanobacterium synechocystis sp. pcc 6803. | molecular chaperones help other proteins in their correct folding and assembly. we have cloned the genes, cpn60 and dnak, which encode proteins belonging to the chaperonin-60 and the 70-kda heat shock protein families from the transformable cyanobacterium synechocystis sp. pcc 6803. these genes are present in single copies in the genome, and the major transcripts for each gene are monocistronic. comparison of deduced amino acid sequences reveals that cyanobacterial chaperonin-60 is equally homol ... | 1991 | 1670771 |
| neurochemical characterization of excitotoxin lesions in the cerebral cortex. | neuronal degeneration that occurs in both ischemia and degenerative neurologic illnesses may involve excitotoxic mechanisms. in the present study, we examined whether cortical lesions with agonists acting at subtypes of glutamate receptors result in selective patterns of neuronal death. injections of quinolinic acid, nmda, homocysteic acid, kainic acid (ka), and alpha-amino-3-hydroxy-5-methylisoxazole-4-proprionic acid (ampa) were made at 2 sites in the dorsolateral frontoparietal cortex in rats ... | 1991 | 1670782 |
| interaction of gut and liver in nitrogen metabolism during exercise. | the role of the gut and liver in nitrogen metabolism was studied during rest, 150 minutes of moderate-intensity treadmill exercise, and 90 minutes of recovery in 18 hour-fasted dogs (n = 6). dogs underwent surgery 16 days before an experiment for implantation of catheters in a carotid artery and in the portal and hepatic veins, and doppler flow cuffs on the hepatic artery and portal vein. arterial glutamine, alanine, and alpha-amino nitrogen (aan) levels decreased gradually with exercise (p less ... | 1991 | 1672034 |
| [effect of sulfasalazine and its metabolites on prostaglandin and leukotriene liberation from human synovial tissue]. | the effects of sulfasalazine (sasp) and its metabolites sulfapyridine (sp) and 5-aminosalicylic acid (5-asa) were investigated on release of prostaglandins (pg) and leukotrienes (lt) from synovial tissue of 37 patients with osteoarthritis, chondrocalcinosis and rheumatoid arthritis. calcium ionophore a23187 significantly increased the release of pge2, 6-keto-pgf1 alpha, ltb4, and ltc4 from human synovial tissue irrespective of the underlying joint disease. sasp inhibited release of ltc4 and incr ... | 1991 | 1673814 |
| identification and properties of an alpha-amylase from a strain of eubacterium sp. isolated from the rat intestinal tract. | 1. a bacterial amylase was isolated from the intestinal content of monoxenic rats inoculated with eubacterium sp. b86. 2. affinity chromatography on cross-linked starch allowed its separation from rat endogenous amylases. 3. the bacterial enzyme was characterized by its pi, molecular weight and action pattern. it behaves as a typical endo-amylase (alpha-amylase). | 1991 | 1673900 |
| the conditions required for the maintenance of onchocerca lienalis microfilariae in vitro. | microfilariae of the bovine parasite onchocerca lienalis were maintained in vitro using a tick cell line as a feeder layer, and under the conditions provided would develop to the sausage stage. a number of media supported this achievement with cmrl 1066 producing the highest yields, particularly when used in conjunction with either medium 199, ham's f-12, rpmi 1640 or mark's m20. the addition of inactivated foetal calf serum (ifcs) suppressed development; on the other hand, the addition of trypt ... | 1991 | 1674403 |
| microbial polysaccharide, hs-142-1, competitively and selectively inhibits anp binding to its guanylyl cyclase-containing receptor. | during the search for anp receptor ligands of microbial origin, we isolated a novel polysaccharide, hs-142-1, from culture broth of aureobasidium sp. hs-142-1 inhibited [125i]-ranp binding to anp receptor in rabbit kidney cortex membranes with an ic50 of 0.3 mu g/ml, but gave no effects on specific binding of [125i]-endothelin nor [125i]-angiotensin ii to their respective receptors in bovine lung membranes. hs-142-1 competitively and selectively inhibited anp binding to its guanylyl cyclase-cont ... | 1991 | 1674870 |
| streptozotocin-induced diabetes in rats causes neuronal deficits in tyrosine hydroxylase and 5-hydroxytryptamine specific to mesenteric perivascular sympathetic nerves and without loss of nerve fibers. | the aim of this study was to investigate the possibility that inadequate venous return to the heart in diabetes is the result of a neuropathy which affects autonomic nerves supplying the splanchnic vasculature. mesenteric veins from rats with streptozotocin-induced diabetes were markedly dilated in vivo compared to veins from control animals. dilation appeared to be the result of loss of muscle tone rather than hypertrophy or hyperplasia of the vessel wall. using quantification by image analysis ... | 1991 | 1675174 |
| phospholipase c genes display restriction fragment length polymorphisms between the genomes of normotensive and hypertensive rats. | the genomic loci of four distinct phospholipase c genes (plc-beta, plc-gamma i, plc-delta and plc-gamma ii) were examined for restriction fragment length polymorphisms (rflps) between the genomes of three normotensive [sprague-dawley, donryu and wistar-kyoto (wky)] and two closely related hypertensive [spontaneously hypertensive (shr) and shr stroke-prone (shr-sp)] rat strains. the rflps observed between shr and wky were classified into three types. type i rflps are those observed at 4.3 kilobas ... | 1991 | 1675227 |
| inhibition of transcription factors belonging to the rel/nf-kappa b family by a transdominant negative mutant. | the kbf1 factor, which binds to the enhancer a located in the promoter of the mouse mhc class i gene h-2kb, is indistinguishable from the p50 dna binding subunit of the transcription factor nf-kappa b, which regulates a series of genes involved in immune and inflammatory responses. the kbf1/p50 factor binds as a homodimer but can also form heterodimers with the products of other members of the same family, like the c-rel and v-rel (proto)oncogenes. the dimerization domain of kbf1/p50 is containe ... | 1991 | 1675604 |
| prevalence of nosocomial infections in a saudi arabian teaching hospital. | the rate of nosocomial infections was determined during a 9-month study in a 6-year-old hospital in north saudi arabia. the overall rate of nosocomial infection in the hospital was 2.2%. the rates in the different services varied. the highest were in the special care baby unit (13.5%) and intensive care unit (6%). in the other services it ranged from 1 to 3.5%. the common causal agents of documented infections were escherichia coli (81), pseudomonas aeruginosa (55), staphylococcus aureus (43), k ... | 1991 | 1675653 |
| regulation of glutamine synthetase activity in the unicellular cyanobacterium synechocystis sp. strain pcc 6803 by the nitrogen source: effect of ammonium. | glutamine synthetase activity from synechocystis sp. strain pcc 6803 is regulated as a function of the nitrogen source available in the medium. addition of 0.25 mm nh4cl to nitrate-grown cells promotes a clear short-term inactivation of glutamine synthetase, whose enzyme activity decreases to 5 to 10% of the initial value in 25 min. the intracellular levels of glutamine, determined under various conditions, taken together with the results obtained with azaserine (an inhibitor of transamidases), ... | 1991 | 1676397 |
| the neuroimmune link in the bronchus-associated lymphoid tissue (balt) of cat and rat: peptides and neural markers. | this light microscopic immunohistochemical study investigates the distribution and target interrelations of nerve fibers in bronchus-associated lymphoid tissues (balt) of rat and cat by using antisera against (1) the polyneuronal marker protein gene product 9.5 (pgp 9.5), (2) selected opioid and nonopioid peptides, and (3) the marker enzymes tyrosine hydroxylase (th) and dopamine beta-hydroxylase (dbh). in both species, a similar distribution pattern of pgp, peptide, and catecholamine enzyme imm ... | 1991 | 1676920 |
| metabolites of fungi & actinomycetes active against mosquito larvae. | extracellular secondary metabolites from 350 fungi and 94 actinomycetes were screened for larvicidal activity against culex quinquefasciatus, anopheles stephensi and aedes aegypti. of them, 133 fungal metabolites and 35 from actinomycetes were active. two from streptomyces sp. and one from paecilomyces sp. were highly active with lc50 value of 1-3 microliters/ml. the metabolites were more toxic to c. quinquefasciatus than to a. stephensi or ae. aegypti larvae. | 1991 | 1677347 |
| characterization of leishmania colombiensis sp. n (kinetoplastida: trypanosomatidae), a new parasite infecting humans, animals, and phlebotomine sand flies in colombia and panama. | characterization of leishmania colombiensis sp.n. is presented, which on the basis of biological and molecular criteria, appears to be a new member of the l. braziliensis complex. a total of nine isolates of the new parasite were made in colombia and panama between 1980 and 1986: two from human cases of cutaneous leishmaniasis, six from phlebotomine sand flies, and one from a sloth. although most closely related to l. lainsoni, l. colombiensis sp.n. is clearly distinguishable from other members ... | 1991 | 1677544 |
| osmoregulatory role of alanine during salt stress in the nitrogen fixing cyanobacterium anabaena sp. 287. | the freshwater cyanobacterium, anabaena sp. 287 exhibited enhanced tolerance to nacl in the presence of ammonium, nitrate, and the amino acids, alanine, valine, proline, lysine, histidine, methionine and aspartic acid. apart from providing permanent protection to the growth during stress, like no3- and nh4+, alanine also relieved the initial salt mediated inhibition on enzymes involved in nitrogen fixation, photosynthesis and respiration. the accumulation of sugars was observed only during molec ... | 1991 | 1677806 |
| staurosporine induces protein kinase c agonist effects and maturation of normal and neoplastic mouse keratinocytes in vitro. | staurosporine is a potent but nonselective inhibitor of protein kinase c (pkc) and blocks responses to 12-o-tetradecanoylphorbol-13-acetate (tpa) in several cell types in vitro. in cultured primary mouse keratinocytes, however, staurosporine fails to inhibit tpa-mediated keratinocyte maturation and itself elicits responses that are similar to tpa (t. sako et al., cancer res., 48: 4646-4650, 1988). after exposure to 10 nm staurosporine for 24 h, essentially all keratinocytes undergo morphological ... | 1991 | 1678684 |
| effects of cannabidiol in animal models predictive of antipsychotic activity. | the effects of cannabidiol (cbd) were compared to those produced by haloperidol in rats submitted to experimental models predictive of antipsychotic activity. several doses of cbd (15-480 mg/kg) and haloperidol (0.062-1.0 mg/kg) were tested in each model. first, cbd increased the effective doses 50% (or) ed50 of apomorphine for induction of the sniffing and biting stereotyped behaviors. in addition, both cbd and haloperidol reduced the occurrence of stereotyped biting induced by apomorphine (6.4 ... | 1991 | 1678894 |
| identification of a minus end-specific microtubule-associated protein located at the mitotic poles in cultured mammalian cells. | a mitosis-specific centrosomal component was studied with a human autoantibody, sp-h, which immunostained mitotic poles and interphase nuclei, and a single polypeptide with an apparent molecular mass of 200 to 230 kda in various lines of cultured cells. early mitotic ptk1 cells treated with 10 micrograms/ml taxol contained short bundles of parallel microtubules around the nuclei and cell periphery. at the time of nuclear envelope breakdown, the nuclear staining by sp-h disappeared, and the antig ... | 1991 | 1679010 |
| four different mutations in codon 28 of alpha spectrin are associated with structurally and functionally abnormal spectrin alpha i/74 in hereditary elliptocytosis. | hereditary elliptocytosis (he) sp alpha i/74 is a disorder associated with defective spectrin (sp) heterodimer self-association and an abnormal tryptic cleavage of the 80-kd alpha i domain of sp resulting in increased amounts of a 74-kd peptide. the molecular basis of this disorder is heterogeneous and mutations in codons 28, 46, 48, and 49 (codons 22, 40, 42, and 43 in the previous nomenclature which did not include the six nh2-terminal amino acids) have been reported. in this study we present ... | 1991 | 1679439 |
| regulation of iron assimilation: nucleotide sequence analysis of an iron-regulated promoter from a fluorescent pseudomonad. | an iron-regulated promoter was cloned on a 2.1 kb bg/ii fragment from pseudomonas sp. strain m114 and fused to the lacz reporter gene. iron-regulated lacz expression from the resulting construct (psp1) in strain m114 was mediated via the fur-like repressor which also regulates siderophore production in this strain. a 390 bp stui-psti internal fragment contained the necessary information for iron-regulated promoter expression. this fragment was sequenced and the initiation point for transcription ... | 1991 | 1679522 |
| prevention of nosocomial aspergillosis. | unfiltered outside air averages 1-15 pathogenic aspergillus sp. colony forming units (cfu) m-3 although short-term fluctuations are substantial. seasonal variation reflects increased spore prevalence during periods of greater availability of non-viable matter. in hospital, airborne spores reflect incomplete filtration, infiltration of outside air and shedding of adherent spores from introduced objects. in highly protected hospital areas supplied with air filtered at high efficiency, where asperg ... | 1991 | 1679817 |
| enzyme-linked immunosorbent assay for the detection and the semi-quantitative determination of taxane diterpenoids related to taxol in taxus sp. and tissue cultures. | an elisa-assay for the detection and the semi-quantitative determination of taxane diterpenoids structurally related to taxol found in taxus sp. has been developed. the antiserum was raised in rabbits using a 2'-succinyltaxol-bovine serum albumin conjugate as immunogen. the working range of the assay was from 1 to 100 ng of taxol. in order to improve the production of taxol, preliminary experiments have been performed on crude extracts of several taxus sp.; the results indicate that the present ... | 1991 | 1680168 |
| axotomy alters putative neurotransmitters in visceral sensory neurons of the nodose and petrosal ganglia. | acute peripheral axotomy of the visceral sensory neurons of the vagus and glossopharyngeal nerves removes peripheral depolarizing and trophic influences to their sensory ganglia. to study axotomy-induced changes in the putative neurotransmitters of visceral sensory neurons, rats were sacrificed 1, 3, 7 or 14 days after transection of either the cervical vagus and superior laryngeal nerves (to affect peripheral axotomy of the nodose ganglion) or the glossopharyngeal and carotid sinus nerves (to a ... | 1991 | 1680528 |
| specific patterns of immunoreactivity in neuronal elements of the anterior major pelvic ganglion of the male guinea-pig. | the anterior major pelvic ganglion (ampg) of the male guinea-pig has been found to consist of three principal components. the presence of a cholinergic component was determined by the demonstration of cytoplasmic and nerve fibre acetylcholinesterase activity. a noradrenergic component was demonstrated by immunoreactivity (ir) of the catecholamine-synthesising enzymes tyrosine hydroxylase (th) and dopamine-beta-hydroxylase (dbh) in neuronal perikarya. the ampg also had a peptidergic component whi ... | 1991 | 1680842 |
| cloning of the human gene for intercellular adhesion molecule 1 and analysis of its 5'-regulatory region. induction by cytokines and phorbol ester. | human intercellular adhesion molecule-1 (icam-1), a specific ligand for the lymphocyte function-associated ag-1 (lfa-1), plays an important role in leukocyte-endothelial cell interactions. it is induced by proinflammatory cytokines such as il-1, tnf-alpha, or ifn-gamma. however, little is known concerning the intracellular regulatory mechanisms which trigger icam-1 up-regulation. in order to study potential regulatory elements involved in icam-1 induction we have cloned the human icam-1 gene and ... | 1991 | 1680919 |
| alpha-adrenergic inhibition of rat pancreatic beta-cell replication and insulin secretion is mediated through a pertussis toxin-sensitive g-protein regulating islet camp content. | the rate of dna synthesis, insulin secretion and camp content in isolated pancreatic islets were markedly inhibited by long-term exposure to the alpha 1-adrenoceptor agonist phenylephrine, the alpha 2-adrenoceptor agonist clonidine and the beta-adrenoceptor antagonist propranolol. pertussis toxin or the stimulatory camp analog sp-camps increased dna synthesis and insulin secretion in the absence of the adrenergic agents. pertussis toxin blocked the inhibitory actions of these agents on dna synth ... | 1991 | 1681806 |
| splotch (sp2h), a mutation affecting development of the mouse neural tube, shows a deletion within the paired homeodomain of pax-3. | the molecular basis of the mouse mutation splotch (sp), which is associated with spina bifida and exencephaly, was analyzed at three of its alleles, sp, sp2h, and spr. we mapped the paired box gene pax-3 within the inha to akp3 interval, near or at the sp locus on chromosome 1, and found pax-3 to be deleted in heterozygous spr/+ mice. analysis of genomic dna and cdna clones constructed from sp2h/sp2h embryos identified a deletion of 32 nucleotides in the pax-3 mrna transcript and gene. this dele ... | 1991 | 1682057 |
| effect of synthetic tachykinin analogues on airway microvascular leakage in rats and guinea-pigs: evidence for the involvement of nk-1 receptors. | 1. the nk-1 selective agonists [beta-ala4, sar9]sp-(4-11) sulphone and [pglu6, pro9]sp-(6-11) dose-dependently increased vascular permeability in various segments of rat and guinea-pig tracheo-bronchial region, while the nk-2 ([nle10]nka-(4-10) and [beta-ala8]nka-(4-10)) or nk-3 ([mephe7]nkb and [mephe7]nkb-(4-10)) selective agonists were inactive. these findings provide evidence that the inflammatory response of the airway to intravenous tachykinins is exclusively mediated by the nk-1 receptor ... | 1991 | 1682326 |
| social deprivation of infant rhesus monkeys alters the chemoarchitecture of the brain: i. subcortical regions. | rhesus monkeys (macaca mulatta) reared during the first year of life without social contact develop persistent stereotyped movements, self-directed behaviors, and psychosocial abnormalities, but neurobiological mechanisms underlying the behaviors of socially deprived (sd) monkeys are unknown. monkeys were reared in total social deprivation for the first 9 months of life; control monkeys were reared socially (sr) with mothers and peers. subjects were killed at 19-24 yr of age. because the behavio ... | 1991 | 1682426 |
| neural control of lower airway vasculature. involvement of classical transmitters and neuropeptides. | 1. sp- and cgrp-ir fibres of presumably sensory origin were abundantly located around the vasculature and glands in the laryngotracheal and bronchial mucosae of the pig. a sparse presence of cgrp- and sp-ir nerves in the tracheobronchial smooth muscle layer as well as an atropine-sensitive bronchoconstrictor response to electrical field stimulation suggested that neuropeptides locally released from sensory nerves may be related to vascular or exocrine gland function rather than to bronchial smoo ... | 1991 | 1683092 |
| in vitro reactivation of in vivo ammonium-inactivated glutamine synthetase from synechocystis sp. pcc 6803. | glutamine synthetase from synechocystis sp. strain pcc 6803 is inactivated by ammonium addition to cells growing with nitrate as the nitrogen source. the enzyme can be reactivated in vitro by different methods such as alkaline phosphatase treatment, but not phosphodiesterase, by raising the ph of the crude extract to values higher than 8, by increasing the ionic strength of the cell-free extract, or by preincubation with organic solvents, such as 2-propanol and ethanol. these results suggest tha ... | 1991 | 1684495 |
| phospholipase c-delta gene of the spontaneously hypertensive rat harbors point mutations causing amino acid substitutions in a catalytic domain. | this study was undertaken in order to investigate the newly discovered spontaneously hypertensive rat (shr)-specific restriction fragment length polymorphism (rflp) at the genomic locus of (poly)phosphoinositide-specific phospholipase c (plc)-delta at a dna sequence level. our aim was to clone the plc-delta complimentary dna (cdna) from shr and analyse the genomic dna obtained from two hypertensive rat strains such as shr and its stroke-prone substrain (shr-sp) and three normotensive rat strains ... | 1991 | 1684614 |
| subsensitivity to beta-adrenoceptor agonists in pacemakers isolated from senescent rats. | 1. the effects of aging on the responsiveness of the isolated pacemaker of the rat to the chronotropic effect of norepinephrine, isoproterenol and soterenol were studied. 2. pacemakers isolated from senescent rats (22-24 months old) showed subsensitivity to norepinephrine, isoproterenol and soterenol, when compared with pacemakers isolated from young rats (3-4 months old). the maximum response to the partial agonist soterenol was reduced. 3. determination of the pa2 value of metoprolol in pacema ... | 1991 | 1684771 |
| identification of a common cyanobacterial symbiont associated with azolla spp. through molecular and morphological characterization of free-living and symbiotic cyanobacteria. | symbiotically associated cyanobacteria from azolla mexicana and azolla pinnata were isolated and cultured in a free-living state. morphological analyses revealed differences between the free-living isolates and their symbiotic counterparts, as did restriction fragment length polymorphism (rflp) analyses with both single-copy glna and rbcs gene probes and a multicopy psba gene probe. rflp analyses with anabaena sp. strain pcc 7120 nifd excision element probes, including an xisa gene probe, detect ... | 1991 | 1685078 |
| asymmetric synthesis of a precursor for the automated radiosynthesis of s-(3'-t-butylamino-2'-hydroxypropoxy)-benzimidazol-2-[11c]one (s-[11c]cgp 12177) as a preferred radioligand for beta-adrenergic receptors. | s-[1-(2,3-diaminophenoxy)]-3'-(n-t-butylamino)propan-2'-ol has been synthesized in three steps from 2,3-dinitro-phenol and the chiral auxiliary, s-glycidyl-3-nitrobenzenesulphonate, to provide a precursor for labelling s-(3'-t-butylamino-2'-hydroxypropoxy)-benzimidazol-2-one (s-cgp 12177) with the short-lived positron-emitting radionuclide, carbon-11 (t 1/2 = 20.4 min; beta+ = 99.8%). reaction of the diamine with [11c]phosgene, itself derived from no-carrier-added cyclotron-produced [11c]methane ... | 1991 | 1685153 |
| detection of lymphocyte subsets using three-color/single-laser flow cytometry and the fluorescent dye peridinin chlorophyll-alpha protein. | the fluorescent dye, peridinin chlorophyll a protein (percp) derived from dinoflagellate organisms (glenodinium sp.) can be excited by a 488 nm laser and emits light with a large stokes shift and no major spectral overlap with commonly used chromophores such as fluorescein isothiocyanate (fitc) and r-phycoerythrin (pe). percp was conjugated directly to various mouse monoclonal antibodies (mab) specific for human leukocyte markers or to avidin for use with biotinylated-mab, and used to perform th ... | 1991 | 1686614 |
| an immunocytochemical analysis of the lateral geniculate complex in the pigeon (columba livia). | the lateral geniculate complex (gl) of pigeons was investigated with respect to its immunohistochemical characteristics, retinal afferents, and the putative transmitters/modulators of its neurons. the distributions of serotonin-, choline acetyltransferase-, glutamic acid decarboxylase-, tyrosine hydroxylase-, neuropeptide y- (npy), substance p- (sp), neurotensin- (nt), cholecystokinin- (cck), and leucine-enkephalin- (l-enk) like immunoreactive perikarya and fibers were mapped. retinal projection ... | 1991 | 1687743 |
| survey of peridomestic mosquito species of jaffna peninsula in sri lanka. | surveys of mosquitos in sri lanka have generally been confined to the south. a recent study in jaffna peninsula in northern sri lanka reported on the biology of peridomestic mosquito species. the present survey indicates the predominant species to be armigeres durhami (edwaards) and armigeres subalbatus (coquillett). aedes albopictus (skuse), ae. aegypti (linnaeus) and ae. novalbopictus barraud were recorded only occasionally during the survey. sampling of ponds in the jaffna peninsula recorded ... | 1991 | 1687931 |
| aquatic macrophytes and the associated mosquitoes in and around madurai city (tamil nadu). | a survey of mosquitoes was carried out in 7 weeded astatic ponds in and around madurai from october 1990 to january 1991 to qualitatively determine the macrophyte-mosquito larvae association. members of culex vishnui subgroup were associated with most of the macrophytes. eichhornia crassipes, marsilea quadrifolia and spirodella polyrhiza showed a high potential for mosquito breeding and azolla sp., a very low potential for both anophelines and culicines. | 1991 | 1688022 |
| streptomyces species producing the streptovaricin complex. | morphological, cultural and physiological-biochemical properties of streptomyces sp. strain 1000 and its antibiotic production were investigated. antibiotics 1011 (identical with the streptovaricin complex) and 1012 (with antibacterial action) were isolated from the cultural broth of this strain. the overproducing natural variant 1011 was isolated from the population of a strain producing antibiotic 1011 at a concentration of 1000 mg/l (activity of the parent strain represents 41 mg/l only). com ... | 1991 | 1688164 |
| homology of the precursor of pulmonary surfactant-associated protein sp-b with prosaposin and sulfated glycoprotein 1. | the precursor of pulmonary surfactant-associated protein, sp-b, is composed of an nh2-terminal domain of 30 residues (a-type domain) and three tandem repeats of about 90 residues (b-type domain); biophysically active mature sp-b corresponds to the second b-type repeat. consensus sequences constructed for the b-type repeats were used to search the data base for homologous sequences, and the search has revealed that prosaposin and sulfated glycoprotein 1 show a remarkable homology with these repea ... | 1991 | 1688355 |
| gonadal steroid regulation of substance p (sp) and sp-encoding messenger ribonucleic acids in the rat anterior pituitary and hypothalamus. | substance p-like immunoreactivity (sp-li) is present in the rat anterior pituitary (ap) and in hypothalamic neurons that may be involved in the control of ap secretion and/or reproductive function. the presence of multiple sp-encoding mrnas and tachykinin peptides and their regulation by steroid hormones were examined in aps and hypothalami from normal, gonadectomized, and steroid-treated male and female rats. sp-encoding mrnas were identified by nuclease protection assays of rna, and tachykinin ... | 1990 | 1688409 |
| disulfiram administration affects substance p-like immunoreactive and monoaminergic neural systems in rodent brain. | the biosynthetic enzyme peptidylglycine alpha-amidating monooxygenase catalyzes the formation of a variety of biologically active alpha-amidated peptides from respective cooh-terminal glycine-extended peptide precursors. peptidylglycine alpha-amidating monooxygenase activity is dependent on copper, ascorbate, and molecular oxygen and is inhibited by the relatively selective copper chelator n,n-diethyldithiocarbamate or its disulfide dimer disulfiram (antabuse). in the present study, chronic disu ... | 1990 | 1688429 |
| peptidase modulation of the pulmonary effects of tachykinins in tracheal superfused guinea pig lungs. | the effects of the angiotensin converting enzyme (ace) inhibitor captopril and the neutral endopeptidase (nep) inhibitors thiorphan and sch 32615 on the changes in airway opening pressure (pao) and the recovery of offered peptide were studied after intratracheal administration of substance p (sp) and neurokinin a (nka) in isolated guinea pig lungs superfused through the trachea. pao changes and the recovery of offered peptide were significantly greater in nep inhibitor-treated lungs than in cont ... | 1990 | 1688568 |
| mapping of the immunodominant regions of the nad-dependent formate dehydrogenase. | a panel of 4 monoclonal antibodies and 7 polyclonal antisera against nad-dependent formate dehydrogenase from methylotrophic bacterium pseudomonas sp. 101 has been obtained. the reactivity of the 37 overlapping proteolytic peptides with the monoclonal antibodies and polyclonal antisera has been studied with elisa test. the data obtained were interpreted residing on the structural model of the formate dehydrogenase at 3 a resolution. the immunodominant regions in the formate dehydrogenase molecul ... | 1990 | 1688814 |
| identification of insulin-like growth factor-binding protein-2 (igf-bp-2) and a low molecular weight igf-bp in human seminal plasma. | three distinct binding proteins for the insulin-like growth factors (igf-bps) have recently had their cdnas cloned and sequenced. we have evaluated igf-bp expression in normal, oligospermic and azospermic human seminal plasma (sp), and have identified igf-bp-2 in all samples. additionally, sp contains a non-glycosylated low molecular weight igf-bp (24k), which does not appear to be structurally or immunologically related to igf-bp-1, -2 or -3. | 1990 | 1688870 |
| neuroimmunomodulation of in vivo anti-rotavirus humoral immune response. | neuropeptides and neurohormones (neurotransmitters) have been shown to modulate immune responses in vitro and in vivo. since reproduction and lactation are regulated by neurohormones, we investigated whether neurohormones could enhance anti-rotavirus immunity in milk. rotavirus-free mice were immunized orally with killed bovine rotavirus (brv) and bred 6 weeks post-immunization. post-whelping, each group of dams (ten mice/group) was given a single injection of prolactin (prl), estrogen, prl and ... | 1990 | 1688878 |
| maturation of neuromodulatory effect of substance p in rabbit airways. | the maturation of the neuromodulatory action of substance p (sp) was investigated in tracheal smooth muscle (tsm) segments isolated from rabbits aged 2-24 wk. the tissues were placed in baths containing krebs-ringer solution and contracted with electrical field stimulation (es) with es frequencies ranging from 1 to 75 hz. in tissues greater than 1 mo of age, the es frequency-response relationships were progressively shifted in the presence of a maximally effective neuromodulatory sp dose (10(-7) ... | 1990 | 1688882 |
| marked regional heterogeneity of 125i-bolton hunter substance p binding and substance p-induced activation of phospholipase c in astrocyte cultures from the embryonic or newborn rat. | the specific binding of 125i-bolton hunter substance p (125i-bhsp) was estimated on 4- to 5-week-old primary cultures of astrocytes from several brain structures and the spinal cord of 16-day-old embryonic or newborn rats. in both cases, high levels of binding of 125i-bhsp were found on intact astrocytes from the brainstem, but this binding was low or negligible on cells from the cerebral cortex, striatum, hypothalamus, and mesencephalon. in addition, hippocampal astrocytes from newborn rats wer ... | 1990 | 1688922 |
| effects of substance p on rate and perfusion pressure in the isolated guinea pig heart. | negative chronotropic and vasodilator responses of the isolated perfused guinea pig heart to substance p (sp) were evaluated. bolus injections of 2.5, 25 and 125 nmol of sp caused a dose-dependent bradycardia, with the largest dose decreasing heart rate by 53% of base line. pretreatment with 1 microm atropine blocked the negative chronotropic response to 25 nmol of sp. pretreatment with 0.5 microm neostigmine lowered base-line heart rate and potentiated the negative chronotropic response to 25 n ... | 1990 | 1688942 |
| nucleotide sequence of the raw-starch-digesting amylase gene from bacillus sp. b1018 and its strong homology to the cyclodextrin glucanotransferase genes. | the gene coding for the raw-starch-digesting amylase from bacillus sp. b1018 was cloned into escherichia coli, and its nucleotide sequence was determined. starting at an atg codon, there was an open reading frame composed of 2139 bp (713 amino acids). the nh2-terminal portion encoded a 27 amino acid-long signal peptide. s1 mapping revealed the presence of a typical promoter region upstream from the transcription initiation site. the deduced amino acid sequence of the extracellular mature enzyme ... | 1990 | 1689153 |
| isolation and identification of a polypeptide in the hsp 70 family that binds substance p. | during the course of an attempt to purify the substance p (sp) receptor from horse salivary glands by substance p-affinity chromatography, a polypeptide of mr = 78,000 was isolated. the first fifteen amino acid residues at the amino terminus were determined and, unexpectedly, were found to be identical with the amino terminus of a glucose-regulated protein (grp) of the same molecular weight, a protein that has been identified as a member of the heat shock protein family. this finding raises the ... | 1990 | 1689156 |
| substance p and neurokinin a, two coexisting tachykinins stimulating the release of [3h]5-ht from rat cerebral cortical slices. | substance p (sp) and neurokinin a (nka), two coexisting neuropeptides of the tachykinin family, stimulated the basal (5 mm k+) and evoked (40 mm k+) release of [3h]5-ht (5-hydroxytryptamine) from tissue slices of the rat cerebral cortex. spantide ([darg1,-dtrp7,9,leu11]sp; 10(-5) m) inhibited the effects of sp but potentiated the effects of nka. the effects of sp and nka appear to be exerted at distinct receptors but involve a common post-receptor mechanism as no full additivity of the sp- and n ... | 1990 | 1689200 |