| enzyme-linked immunosorbent assay for the differential diagnosis of pulmonary tuberculosis and pulmonary diseases due to mycobacterium avium-intracellulare complex. | we studied the usefulness of antibody estimation by the enzyme-linked immunosorbent assay (elisa) technique in the differential diagnosis of pulmonary mycobacterial diseases. sera from patients with active pulmonary diseases caused by mycobacterium tuberculosis (n = 42) or mycobacterium avium-intracellulare complex (mai) (n = 26) and from healthy controls (n = 75) were assayed for igg and iga antibody titers to antigen of m. tuberculosis and mai. the igg and iga antibody titers to m. tuberculosi ... | 1989 | 2733244 |
| [investigation of severe tuberculous pneumonia associated with irreversible exacerbation after intensive antitubercular chemotherapy--with regard to the etiology of initial reversible roentgenographic progression]. | we reported 3 cases of severe tuberculous pneumonia associated with irreversible exacerbation and sequential death appeared after each intensive antitubercular chemotherapy containing inh, rfp, eb and sm. acute tuberculous pneumonia probably represents an exudative hypersensitivity reaction to tuberculoprotein, rather than actual inflammation caused by the mycobacterium tuberculosis organism. mechanisms of the reversible roentgenographic progression are considered to be also similar to those of ... | 1989 | 2733304 |
| [experimental study of the antitumor effect of z-100 in the treatment of mm46 tumor transplanted in c3h/he mice. 2. effect of the combination therapy of radiation and long term administration of z-100]. | female c3h/he mice aged 14 weeks with transplanted mm46 tumor were used to investigate the effect of an immunomodulator, z-100 (an arabinomannan lipid extracted from mycobacterium tuberculosis strain aoyama b) combined with local irradiation of 30 gy (3,000 rad). daily doses of 5 micrograms, 50 micrograms and 500 micrograms/kg of z-100 were injected intramuscularly for 14 consecutive days after irradiation, and 2 times a week for 6 weeks thereafter. the antitumor effect was evaluated by the chan ... | 1989 | 2738440 |
| a prospective hospital study of the aetiology of community-acquired pneumonia. | in a one-year prospective study of 106 adults (mean age, 60 years) who were admitted to hospital with community-acquired pneumonia, an aetiological diagnosis was made in 82 (77%) patients. streptococcus pneumoniae was considered to be responsible for 44 (42%) and respiratory viruses for 19 (18%) infections. other aetiological agents that were found in a smaller number of patients included haemophilus influenzae (9% of patients), enteric gram-negative bacilli (8% of patients), staphylococcus aure ... | 1989 | 2739613 |
| influence of t-2 toxin on bcg vaccine efficacy against murine pulmonary tuberculosis. | unvaccinated mice were treated with t-2 toxin either 6 days before or 1 day after exposure to aerosols of virulent mycobacterium tuberculosis. mice were also treated with t-2 toxin 6 days before vaccination with mycobacterium bovis (bcg). there was a t-2 toxin mediated increase in the number of viable vaccine organisms recovered from the spleens of toxin-treated mice at 3 weeks after vaccination, and splenomegaly at post-vaccine weeks 3 through 5. at the seventh week after vaccination, toxin-tre ... | 1989 | 2741306 |
| [relation between the chemical structure of substances and their antimicrobial action against atypical strains. ii. 6-acycloamido-2-alkylthiobenzothiazoles, quantitative relation to their effectiveness spectrum]. | forty-six 6-acylamido-2-alkylthiobenzothiazoles were tested in vitro for antimicrobial activity towards mycobacterium avium. the values of logarithms of the minimal inhibitory concentrations (log mic) are shown in table 1. the relationships between chemical structure and the activity under study were studied by the free-wilson method. the results, contributions of the substituents in positions 2 and 6 and the contribution of the common benzothiazole part (marked c) to the activity (delta log mic ... | 1989 | 2743430 |
| synchronous mycotic aneurysms secondary to tuberculosis. | mycotic aneurysm secondary to mycobacterium tuberculosis is extremely unusual. we describe a 28-year-old female patient with tuberculosis who developed synchronous mycotic aneurysms of the abdominal aorta and the innominate artery. the pathogenesis, diagnosis, and treatment of tuberculous mycotic aneurysm is reviewed. | 1989 | 2754310 |
| [fever, productive cough, night sweats]. | a 43 year old male complained about fever, productive cough and nocturnal sweating. he was treated for pneumonia with antibiotics because of pulmonary infiltrate. tomography revealed a large cavity with infiltrated border in the right inferior lobe. by means of bronchoscopy the diagnosis of bronchial tuberculosis was established. mycobacterium tuberculosis was cultured. a comparison with previous radiographs suggested a duration of the disease of approximately 3 years. a combined chemotherapy wi ... | 1989 | 2756267 |
| peritoneal dialysis-associated tuberculous peritonitis in an intravenous drug user with acquired immunodeficiency syndrome. | a rare case of tuberculous peritonitis in a continuous ambulatory peritoneal dialysis (capd) patient who has multiple risk factors for extrapulmonary disease due to mycobacterium tuberculosis is presented. this patient' acute course was atypical with a predominance of neutrophils and low levels of protein in the peritoneal fluid. obtaining the diagnosis of tuberculous peritonitis by acid-fast smear was also unusual, probably facilitated by centrifugation of large amounts of fluid. the patient wa ... | 1989 | 2757020 |
| disseminated lupus vulgaris presenting as granulomatous folliculitis. | a 69-year-old man presented with a 3-year history of scattered, grouped, asymptomatic follicular papules, pustules, and nodules tending toward coalescence into large geographic aggregates. repeated cutaneous biopsy specimens showed granulomatous folliculitis with negative ziehl-neelsen stains. finally, biopsy material submitted for mycobacterial culture grew mycobacterium tuberculosis. no evidence of active systemic tuberculosis was found. the patient had a very rare form of tuberculosis, dissem ... | 1989 | 2767838 |
| cerebrospinal fluid concentrations of ethionamide in children with tuberculous meningitis. | cerebrospinal fluid ethionamide concentrations were determined in 18 children (median age 26.5 months) with tuberculous meningitis complicated by raised intracranial pressure. lumbar spinal fluid specimens were obtained before and after weekly hour-long monitoring of intracranial pressure. thirty-five paired and four single specimens were evaluated. a dosage schedule of 15 mg/kg was used on 26 occasions, and a spinal fluid ethionamide concentration of 2.5 micrograms/ml, the in vitro minimal inhi ... | 1989 | 2769511 |
| tuberculosis on regular hemodialysis--a case of pericardial tamponade. | the patient presented in this paper had been stable for 3 months after the induction of hemodialysis, when nausea, vomiting and hepatomegaly suddenly developed. a chest film revealed rush cardiomegaly, and massive pericardial effusion was demonstrated by echocardiography. one liter of hemorrhagic fluid was removed by pericardiocentesis and subsequent pericardial drainage under echocardiography. the patient received chemotherapy against pulmonary tuberculosis 30 years ago and calcification on che ... | 1989 | 2769929 |
| tuberculous retropharyngeal abscess producing respiratory obstruction. | oropharyngeal tuberculosis is a rare presentation of the disease even in the presence of extensive pulmonary tuberculosis. a case is described in which a retropharyngeal abscess, causing stridor and threatening respiratory obstruction, was the only manifestation of mycobacterium tuberculosis infection. | 1989 | 2772861 |
| [in vitro determination of the sensitivity of mycobacteria to fluoroquinolones]. | in vitro activity of four fluorinated quinolones: pefloxacin, norfloxacin, ciprofloxacin, ofloxacin were determined against various clinical isolates of mycobacteria. the method of agar dilution was used, seventy strains of ten species were tested: mycobacterium tuberculosis (25), mycobacterium bovis (5), mycobacterium africanum (2), bcg (5), mycobacterium kansasii (6), mycobacterium marinum (5), mycobacterium avium (11), mycobacterium xenopi (6), mycobacterium chelonae (3), mycobacterium fortui ... | 1989 | 2780092 |
| [patients hospitalized with pulmonary tuberculosis presenting koch's bacillus in direct sputum examination. clinical, radiologic and bacteriologic characteristics]. | to determine the influence of socio-economic, clinical and radiological factors on the detection of a mycobacterium tuberculosis-positive direct sputum smear, we studied 531 patients hospitalized for pleuropulmonary tuberculosis over a 5 year period. m. tuberculosis positivity of the expectorate smear was found more frequently in clinically detected (43%) than in radiologically detected (26%) tuberculosis (p less than 0.001). on admission, a m. tuberculosis-positive sputum smear was more common ... | 1989 | 2782769 |
| skin test responsiveness to mycobacterium tuberculosis, hla-dr4, and rheumatoid arthritis. | | 1989 | 2784660 |
| immune reactions in patients with superficial bladder cancer after intradermal and intravesical treatment with bacillus calmette-guérin. | the immune reactivity of patients with strongly recurrent superficial bladder cancer was followed after combined intravesical and intradermal bacillus calmette-guérin (bcg) immunotherapy. all patients in this study were previously treated without success with intravesical chemotherapy. the bcg treatment regimen consisted of weekly administrations with bcg (rivm) for six consecutive weeks, both intravesically and intradermally. in this study, sera and peripheral blood leukocytes (pbl) of patients ... | 1989 | 2784716 |
| in vitro analysis of the cellular mechanisms involved in immunity to tuberculosis. | tuberculosis is a chronic infectious disease caused by mycobacterium tuberculosis. protection against and pathogenesis of tuberculosis greatly depend on specific t lymphocytes, and it is generally assumed that cd4+ t cells--through lymphokine-mediated macrophage activation--are the major mediators of the host response to tuberculosis. in the present report, results from experimental tuberculosis studies in mice are summarized which indicate that both cd4+ and cd8+ t lymphocytes are generated dur ... | 1989 | 2785283 |
| brain and spinal cord levels of histamine in lewis rats with acute experimental autoimmune encephalomyelitis. | acute experimental autoimmune encephalomyelitis (eae) was induced in lewis rats by inoculation with guinea pig spinal cord homogenate emulsified with mycobacterium tuberculosis-enriched complete freund's adjuvant (cfa). control rats were inoculated with cfa alone. control and eae rats were killed on days 7, 9, 11, and 13 postinoculation, and regional brain and spinal cord levels of histamine were determined. no regional differences in histamine content between control and eae rats were seen on d ... | 1989 | 2786054 |
| chronic experimental allergic neuritis in lewis rats. | an experimental chronic relapsing demyelinating neuropathy was produced by immunizing adult lewis rats with bovine myelin in low (2.5 mg) and high (5 mg) doses, with and without mycobacterium tuberculosis in the adjuvant. each regime produced a similar disease course: acute severe hind limb weakness was followed by apparent recovery and then reappearance of mild neurological deficit with occasional spontaneous exacerbations. the partially recovered animals were relatively resistant to reimmuniza ... | 1989 | 2787483 |
| origin and fate of ige-bearing lymphocytes. ii. modulation of ige isotype expression on peyer's patch cells by feeding with certain bacteria and bacterial cell wall components or by thymectomy. | mechanisms regulating the appearance of sige+ b lymphocytes appear to be lacking in adult germfree (gf) rats in that their peyer's patches (pp) contain high numbers of cells with sige (approximately 15% of total cells), one-half of which simultaneously express siga, whereas sige+ cells are absent from pp of conventional rats (less than 1%). gf rat pp also contain elevated numbers of siga+ cells and decreased numbers of sigm+ cells, with elevated numbers of sthy-1+ rt 7.1+ ig- t cells, and reduce ... | 1989 | 2789249 |
| high incidence of pulmonary tuberculosis in the non-hiv infected immunocompromised patients in hong kong. | in areas where tuberculosis is endemic, clinicians managing immunocompromised patients (icp) are confronted with the possibility of mycobacterium tuberculosis as a pathogen. to determine the incidence and clinical pattern of, the diagnostic approach to, and potential therapeutic implications of pulmonary tuberculosis in this patient population, we reviewed 62 non-hiv infected icp in hong kong who had bronchoscopy because of pulmonary infiltrates. pulmonary tuberculosis was the second most common ... | 1989 | 2791681 |
| mycobacterium tuberculosis infection in foreign born adoptees. | korean adoptees placed by associated catholic charities of baltimore between january, 1985, and december, 1988 (n = 873), underwent surveillance for tuberculosis (tb). overall tuberculin reactivity or active tb was documented in nine placements. two children had active disease; both were skin test-negative and one died of tuberculous meningitis. the median age of the seven asymptomatic tuberculin reactors, none of whom had received bacillus calmette-guérin vaccination, was 1 year. this reactivit ... | 1989 | 2797959 |
| [pulmonary manifestations in patients with aids]. | in acquired immunodeficiency syndrome (aids) the pulmonary opportunistic infections are due to the depression of cellular immunity and they are found in more than 50% of patients. most frequently the infection is due to pneumocystis carinii, cytomegalovirus, cryptococcus neoformans and mycobacterium avium-intracellulare. non-opportunistic infections in aids are mostly due to the mycobacterium tuberculosis and legionella pneumophila. in kaposi sarcoma in aids the lungs may be involved into pulmon ... | 1989 | 2798562 |
| [esophagobronchial fistula of tuberculous origin]. | we present the case of an asymptomatic male in whom bilateral hilar and right paratracheal lymph node enlargement was a casual finding. an esophagogram was performed to evaluate possible mediastinal nodes and an esophagobronchial fistula was discovered. in view of the coexistence of lymph node enlargement and esophagobronchial fistula, the case was presumed to be tuberculosis, as was confirmed by sputum culture positive for mycobacterium tuberculosis. after tuberculostatic treatment, the course ... | 1989 | 2799038 |
| activity and penetration of antituberculosis drugs in mouse peritoneal macrophages infected with mycobacterium microti ov254. | the activities of some commonly used antituberculosis drugs were investigated within unstimulated peritoneal macrophages and in 7h-9 medium without tween 80 using mycobacterium microti ov254 as the target organism. in macrophage cultures, serial concentrations of isoniazid, rifampin, pyrazinamide, or streptomycin were added after a 2.5-h phagocytosis period. viable counts were carried out at daily intervals for 5 or 6 days. the patterns of susceptibility to the four drugs were similar for m. mic ... | 1989 | 2802553 |
| the clinical manifestations of the tuberculous pleural effusion in adult patients. | the clinical data of 52 patients with tuberculous pleural effusion were analyzed. their average age was 48 years old. thirty two (62%) of them were sick for less than one week. fever, chest pain and cough were the predominant pictures. six of the 52 patients had coexisting disease. intermediate strength protein purified derivative (ppd) test was found to be positive in 14 out of 31 (45%) patients. their pleural effusions were usually unilateral, right-sided, and nearly half of them occupied more ... | 1989 | 2804770 |
| immunosuppression and mycobacteria other than mycobacterium tuberculosis: results from patients with and without hiv infection. | infections caused by mycobacteria other than mycobacterium tuberculosis (mott) have often been described as common in aids patients. to evaluate whether infections with mott are specific for hiv related immunosuppression or are also frequent in patients with immunosuppression of different aetiology, data on the frequency of isolation from immunosuppressed patients with hiv infection are important. blood, stool and urine specimens from 134 patients with non-hiv related immunosuppression, and from ... | 1989 | 2806416 |
| immunohistologic analysis of mycobacterial antigens by monoclonal antibodies in tuberculosis and mycobacteriosis. | four monoclonal antibodies (moabs), 60.15, 61.3, 105.10, and 2.16, directed to different proteins of mycobacterium tuberculosis were used by an indirect avidin-biotin complex peroxidase-antiperoxidase method to detect mycobacterial antigens in lung, lymph node, and joint tissue specimens of tuberculous patients. using moab 60.15, which recognizes a broad range of cross-reactive mycobacterial proteins with a molecular mass of 28 kilodaltons (kd), scattered materials (mycobacterial in origin) were ... | 1989 | 2807270 |
| [evaluation of clinical efficacy of isoniazid and ethambutol in the treatment of nontuberculous mycobacteriosis based on in vitro susceptibility testing]. | comparing minimal inhibitory concentrations (mics) determined in various nontuberculous mycobacteria with those for mycobacterium tuberculosis strains, which were isolated from patients who were untreated previously by any antituberculosis drugs, clinical efficacy of isoniazid and ethambutol in the treatment of nontuberculous mycobacteriosis was evaluated. the mics of isoniazid for m. tuberculosis strains were 0.03-0.1 microgram/ml, whereas the mics for m. xenopi strains were 0.1-0.4 microgram/m ... | 1989 | 2811008 |
| genes for immunodominant protein antigens are highly homologous in mycobacterium tuberculosis, mycobacterium africanum, and the vaccine strain mycobacterium bovis bcg. | the relatedness of immunodominant protein antigens in mycobacterium tuberculosis, m. africanum, and m. bovis bcg was investigated by comparing the genes that encode major protein antigens in m. tuberculosis with their counterparts in the other two mycobacteria. genes encoding homologs of m. tuberculosis major protein antigens were isolated from m. africanum and m. bovis bcg by constructing lambda gt11 recombinant dna expression libraries and screening them with murine monoclonal antibodies and d ... | 1987 | 2820881 |
| capacity of human neutrophils to kill mycobacterium tuberculosis. | | 1987 | 2824628 |
| inhibition of macrophage priming by sulfatide from mycobacterium tuberculosis. | sulfatide from the outer surface of mycobacterium tuberculosis blocked priming in cultured human monocytes. monocytes were primed in vitro with either lipopolysaccharide (lps) or interferon-gamma. primed monocytes released increased amounts of superoxide anion (o2-) when stimulated with formyl-methionyl-leucyl-phenylalanine or with phorbol myristate acetate. primed monocytes also showed increased phagocytosis of sheep erythrocytes and increased release of interleukin 1. when primed monocytes wer ... | 1988 | 2826597 |
| rifabutine: minimal inhibitory and bactericidal concentrations for mycobacterium tuberculosis. | the minimal inhibitory concentrations (mic) and minimal bactericidal concentrations (mbc) of rifabutine (ansamycin lm427, spiropiperidyl rifamycin) against rifampin-susceptible and rifampin-resistant strains of mycobacterium tuberculosis were determined. the mics for 17 rifampin-susceptible strains were low, falling in a narrow range of 0.06 microgram/ml or less when determined by either the agar-dilution method (in 7h10 or 7h11 agar) or in 7h12 broth (radiometrically or by sampling and colony-f ... | 1988 | 2830815 |
| [is there a structural identity between sensitive strains of m. tuberculosis isolated from new patients and those from patients in recurrence and relapse following current initial chemotherapy?]. | | 1987 | 2831614 |
| lung cancer, tuberculin reactivity, and isoniazid. | we determined the incidence of tuberculosis in lung cancer patients with known tuberculin status. all patients received chemotherapy with or without radiation therapy, corticosteroid therapy, or both, and none received isoniazid prophylaxis. positive tuberculin reactivity was found in 89 of the 257 patients; among these 89, tuberculosis developed in one patient before and in one patient after chemotherapy. among the other 168 patients, one case of tuberculosis developed after chemotherapy. for a ... | 1988 | 2831628 |
| in vitro activity of new rifamycins against rifampicin-resistant m. tuberculosis and mais-complex mycobacteria. | comparisons were made of the in vitro activity of rifampicin, and the rifamycin derivatives, rifapentine, rifabutin, cgp 29861, cgp 7040 and cgp 27557, against rifampicin-sensitive and rifampicin-resistant strains of mycobacterium tuberculosis and against the mycobacterium avium/intracellulare/scrofulaceum (mais) complex. the new rifamycins had mics four to eight times lower than those of rifampicin against sensitive m. tuberculosis strains. of the 35 rifampicin-resistant strains of m. tuberculo ... | 1987 | 2834842 |
| in vitro observations on the suitability of new rifamycins for the intermittent chemotherapy of tuberculosis. | the bactericidal activity of six new rifamycin derivatives--rifabutin (rbu), fce 22250 (f22), rifapentine (rpe), cgp 29861 (c29), cgp 7040 (c70) and cgp 27557 (c27) and rifampicin (rmp)--have been measured against log phase and, as a better test of sterilising activity, against stationary phase cultures of mycobacterium tuberculosis, h37rv. the order of activity of 1.0 and 0.2 mg/l rifamycin against log phase cultures was rmp greater than rpe & c27 greater than rbu & c29 greater than c70. the or ... | 1987 | 2834843 |
| [in vitro activities of rifabutine and rifampicin against various mycobacteria, especially mycobacterium tuberculosis and mycobacterium avium complex]. | | 1988 | 2836649 |
| cloning and expression of the mycobacterium bovis bcg gene for extracellular alpha antigen. | the gene for the extracellular alpha antigen of mycobacterium bovis bcg was cloned by using a single probe restricted to g or c in the third position. this technique should have great potential for the isolation of mycobacterial antigen genes. the gene analysis revealed that the alpha antigen gene encoded 323 amino acid residues, including 40 amino acids for signal peptide followed by 283 amino acids for mature protein. this is the first report on the structure of the mycobacterial signal peptid ... | 1988 | 2842287 |
| lysogeny and transformation in mycobacteria: stable expression of foreign genes. | requisite to a detailed understanding of the molecular basis of bacterial pathogenesis is a genetic system that allows for the transfer, mutation, and expression of specific genes. because of the continuing importance of tuberculosis and leprosy worldwide, we initiated studies to develop a genetic system in mycobacteria and here report the use of two complementary strategies to introduce and express selectable genetic markers. first, an escherichia coli cosmid was inserted into the temperate myc ... | 1988 | 2842799 |
| effect of mycobacterium tuberculosis-derived sulfolipid i on human phagocytic cells. | experiments were performed to determine the effects of mycobacterium tuberculosis-derived sulfolipid i on phagocytic cells. sulfolipid i was taken up in significant amounts by human neutrophils and in lesser amounts by monocytes and lymphocytes. superoxide (o2-) production by neutrophils was significantly increased by sulfolipid i, but the rate of production was slower than that reported previously for other stimuli. the optimal concentration of sulfolipid i for stimulation of o2- production was ... | 1988 | 2844675 |
| protective immunity to experimental tuberculosis by mannophosphoinositides of mycobacteria. | mannophosphoinositides isolated from mycobacterial cells were found to induce both humoral and cell-mediated immune responses in mice when injected as mannoside-methylated bovine serum albumin (mbsa) complexes. immunization of mice with mannoside-mbsa complexes elicited significant protection against challenge with ld50 dose of m. tuberculosis h37rv as revealed by high survival rate, low values of root-specific lung weight, lung densities and colony forming units recovered from lung, liver and s ... | 1988 | 2845237 |
| serodiagnostic potentialities of enzyme-linked immunosorbent assay (elisa) using mannophosphoinositides of mycobacterium tuberculosis h37rv. | the serological response to mannophosphoinositides of mycobacterium tuberculosis h37rv and to tuberculin-purified protein derivative (ppd) was examined by enzyme-linked immunosorbent assay (elisa) in patients suffering from tuberculosis and related diseases. in sputum positive cases 94% samples were found to be positive to mannoside antigens and 77% to ppd, while in sputum negative cases, 71% of samples gave a positive reaction to mannosides and 54% to ppd. the high specificity of mannoside elis ... | 1988 | 2845238 |
| [study of the minimal inhibitory concentration of rifabutine (ansamycin lm 427) for mycobacterium tuberculosis, mycobacterium xenopi and mycobacterium avium-intracellulare]. | ansamycin lm 427 (or rifabutine) is a spiropiperidyl-rifamycin which has been proposed as a treatment for tuberculosis and mycobacterial infections resistant to rifampicin. the minimal inhibitory concentration (mci) of rifabutine and of rifampicin for 10 strains of m. tuberculosis sensitive to rifampicin, 15 strains of m. tuberculosis resistant to rifampicin, 10 strains of m. xenopi and 15 of m. avium-intracellulare have been measured in a comparative fashion on gel difco 7h11 medium and also on ... | 1988 | 2845531 |
| [results of bacteriologic follow-up in the ambulatory treatment of tuberculosis in timiş county]. | | 1988 | 2849187 |
| antimycobacterial activity in vivo of lm427 (rifabutin). | the activity of the rifampin derivative rifabutin (ansamycin; lm427) was tested in vivo against experimental infections of mice with members of the mycobacterium avium complex (mac) and a highly virulent strain of mycobacterium tuberculosis. rifabutin was moderately effective against an environmental serotype 8 strain of mac and of significant effectiveness against low dose aerogenic infections with two strains of higher virulence. the drug was also highly effective in causing the rapid resoluti ... | 1988 | 2849349 |
| aids and dentistry: a complex challenge. | | 1988 | 2850945 |
| effect of lymphokines on b-cell responses to mycobacterium tuberculosis: a comparison of in vitro secreted antibodies by epstein barr virus transmitted b-cell and non-transformed peripheral blood b-cells from the same donor. | | 1988 | 2853043 |
| restriction endonuclease analysis of total deoxyribonucleic acid of mycobacterium tuberculosis h37rv (atcc 27294) and of m. bovis (atcc 19210). | total dna from two slowly-growing pathogenic mycobacterial species propagated in vitro was isolated, digested with each of 34 restriction endonucleases and analyzed by agarose gel electrophoresis. the most distinct profiles for m. tuberculosis (atcc 27294) and for m. bovis (atcc 19210) were obtained respectively using (bamhi, drai, clai, ecori, ecorv, hindiii, hpai, sali, smai, xbai, and xmai). the patterns produced for these strains were reproducible and distinguishable from each other. however ... | 1988 | 2854960 |
| [the results of bacteriologic research on the bronchoalveolar lavage fluid and blood plasma in patients with sarcoidosis]. | | 1988 | 2855668 |
| [a comparison of the immediate results of intensive short-term antitubercular chemotherapy with those of the classic regimens lasting 9 months]. | | 1988 | 2855672 |
| [current concerns in the field of tuberculosis immunology]. | | 1988 | 2855758 |
| [evaluation of the weight of predictive criteria in the evolution of pulmonary tuberculosis]. | | 1988 | 2855760 |
| [biochemical profile of mycobacteria isolated from clinical and environmental material]. | biochemical characteristics employed to identify tuberculous and non tuberculous mycobacteria were evaluated. tests for detection of nitrate reductase, catalase, gamma-glutamyl-transferase, niacin assay and growth on tch media (2-thiophene carboxylic-acid hydrazide) were regarded of discriminating value. these tests have been applied to identification of 78 clinical isolates of mycobacteria. all strains were identified as mycobacterium tuberculosis, that suggest the infrequency of human infectio ... | 1985 | 2867581 |
| a carrier method for the assessment of the effectiveness of disinfectants against mycobacterium tuberculosis. | a method for testing the effect of disinfectants against mycobacterium tuberculosis was developed using glass slide carriers contaminated with myco. tuberculosis from spleens of guinea-pigs. the method was found to be useful although a greater number of colony forming units, permitting calculation of a reduction factor in the range of 10(5), is desirable. in tests with various disinfectants, alcohols were the most effective agents against myco. tuberculosis. | 1986 | 2870110 |
| t lymphocytes of rheumatoid arthritis patients show augmented reactivity to a fraction of mycobacteria cross-reactive with cartilage. | an acetone-precipitable fraction of mycobacterium tuberculosis cross-reacts with human cartilage. immune responses to this antigen were assessed in 34 patients with rheumatoid arthritis, 16 patients with degenerative joint disease, and 15 healthy controls. the ra patients differed from the other two groups in having more pronounced t lymphocyte responses to the antigen; their serum antibody levels were not higher. the responses of ra patients varied with duration of disease. in the first year (7 ... | 1986 | 2874329 |
| rheumatoid arthritis and tuberculosis. | | 1986 | 2876157 |
| rheumatoid arthritis and tuberculosis. | | 1986 | 2876272 |
| diagnosis of tuberculous meningitis by detection of tuberculostearic acid in cerebrospinal fluid. | tuberculostearic acid, a structural component of mycobacterium tuberculosis, was identified by gas chromatography/mass spectrometry with selected ion monitoring in cerebrospinal fluid (csf) from 13 patients with proven and 8 out of 9 patients with suspected tuberculous meningitis; the negative result was in a patient whose symptoms and csf abnormalities may have been due to systemic lupus erythematosus. tuberculostearic acid was found in the csf of only 1 patient out of 87 with non-tuberculous m ... | 1987 | 2885596 |
| comparative testing of disinfectants against mycobacterium tuberculosis and mycobacterium terrae in a quantitative suspension test. | in order to establish a quantitative suspension test to assess the tuberculocidal activity of disinfectants, comparative tests were carried out with a variant of the dutch standard suspension test using mycobacterium tuberculosis and mycobacterium terrae as the test organisms. the results indicate that both species have a similar susceptibility towards disinfectants. because of this and since m. terrae is a relatively fast growing organism with a low pathogenic potential, it is recommended that ... | 1987 | 2891759 |
| differential effect of bordetella pertussis on experimental posterior uveitis in the black-hooded lister rat. | the effect of an additional adjuvant, bordetella pertussis, on the clinical and histopathologic features of experimental autoimmune uveitis in black-hooded lister rats was investigated. disease was induced by a single footpad injection of purified retinal s-antigen in freund's complete adjuvant. in those animals that did not receive b pertussis the clinical features were those of a retinal vasculitis with disc edema, periphlebitis, and deep retinal infiltrates. in contrast, animals that received ... | 1988 | 2892482 |
| the mycobacterium tuberculosis 65-kilodalton antigen is a heat shock protein which corresponds to common antigen and to the escherichia coli groel protein. | monoclonal hybridoma antibodies directed against a 65-kilodalton (kda) mycobacterial protein could detect similarly sized antigens in many other bacterial species. in pseudomonas aeruginosa, the cross-reacting protein corresponded to a 62-kda antigen that has been called common antigen. the mycobacterial 65-kda antigen and common antigen are similar in that both (i) are highly immunoreactive molecules, (ii) contain species-specific and genus-specific epitopes in addition to the broadly cross-rea ... | 1988 | 2892795 |
| molecular properties and regulation of mrna expression for murine t cell-replacing factor/il-5. | we previously cloned cdna for a t cell-replacing factor (trf) that has been defined as a t cell-derived lymphokine that acts on activated b cells as a b cell growth and differentiation factor. based on the diverse activities of rtrf on different target cells, we proposed that trf be called il-5. in this study, the molecular characteristics of trf/il-5 prepared by rdna technology and trf/il-5 mrna expression in various t cell lines and normal t cells have been studied. specific immunoassay showed ... | 1988 | 2893824 |
| mycobacterial cross-contamination of bronchoscopy specimens. | | 1988 | 2895146 |
| the prevention and control of mycobacterial infections in hospitals. | infections resulting from contamination of medical equipment and medications with opportunistic mycobacteria are reviewed and the mode of spread discussed. the prevention of mycobacterium tuberculosis in patients and staff, by the implementation of occupational health schemes, infection control policies and disinfection policies is also reviewed. the problems relating to immunocompromised patients are also discussed. | 1988 | 2896738 |
| infection control during neutropenia. | the first principle of infection prevention in neutropenic patients is to ensure that every effort is made to prevent impairment of the host's defences or any disturbance of the ecological balance of the patient's microbial flora. the second principle is that potential or established sources of infection should be sought and ideally treated before any immunosuppressive therapy is instituted. the third principle is to define the extent to which a particular patient can be expected to benefit from ... | 1988 | 2896750 |
| controlled clinical trial of complete open surgical drainage and of prednisolone in treatment of tuberculous pericardial effusion in transkei. | 240 patients with active tuberculous pericardial effusion received a 4-drug daily antituberculosis regimen for 6 months and have been studied for 24 months or longer. those willing were randomly allocated to open pericardial biopsy and complete drainage of pericardial fluid on admission or percutaneous pericardiocentesis as required. all patients were randomly allocated to prednisolone or matching placebo for the first 11 weeks, on a double-blind basis. complete open drainage on admission abolis ... | 1988 | 2901610 |
| a major antigen from mycobacterium tuberculosis which is homologous to the heat shock proteins groes from e. coli and the htpa gene product of coxiella burneti. | | 1988 | 2902558 |
| the recombinant 65-kd heat shock protein of mycobacterium bovis bacillus calmette-guerin/m. tuberculosis is a target molecule for cd4+ cytotoxic t lymphocytes that lyse human monocytes. | since little is known about tc cells in the human immune response to intracellular parasites, we have studied the role of tc cells in response to m. bovis bacillus calmette-guerin (bcg). donors whose pbmc responded to bcg, purified protein derivative (ppd), and the recombinant 65-kd heat shock protein (hsp) of bcg generated bcg/ppd-specific cd4+ effector t lymphocytes that lysed ppd as well as recombinant 65-kd-pulsed monocytes. nonpulsed or irrelevant antigen-pulsed target cells were lysed to a ... | 1988 | 2903217 |
| five-year follow-up of a clinical trial of three 6-month regimens of chemotherapy given intermittently in the continuation phase in the treatment of pulmonary tuberculosis. singapore tuberculosis service/british medical research council. | in a study in singapore, patients of chinese, malay, and indian ethnic origin with sputum-smear-positive pulmonary tuberculosis were allocated at random to daily treatment with streptomycin, isoniazid, rifampin, and pyrazinamide for 2 months (2shrz), for 1 month (1shrz), or for 2 months without streptomycin (2hrz), followed, for all patients, by 3-times-weekly isoniazid and rifampin (h3 r3) up to 6 months. as previously reported, all except 1 of 319 patients with drug-susceptible tubercle bacill ... | 1988 | 2904237 |
| [antimycobacterial properties of antihistaminic compounds]. | | 1988 | 2906237 |
| increasing incidence of tuberculosis in a prison inmate population. association with hiv infection. | the incidence of tuberculosis (tb) among inmates of the new york state prison system increased from 15.4 per 100,000 in 1976 through 1978 to 105.5 per 100,000 in 1986. matching of tb and acquired immunodeficiency syndrome registries indicated that the majority (56%) of inmates with tb reported in 1985 and 1986 had acquired immunodeficiency syndrome or human immunodeficiency virus infection; none were known to be human immunodeficiency virus seronegative. a case-control study examined 59 inmates ... | 1989 | 2909778 |
| use of a cutoff range in identifying mycobacteria by the gen-probe rapid diagnostic system. | commercial dna probes (gen-probe corp., san diego, calif.) for mycobacterium tuberculosis complex mycobacterium avium, and mycobacterium intracellulare were compared with conventional methods for accuracy, applicability, and speed for the identification of putative isolates of the m. tuberculosis and m. avium complexes. results are expressed as percent hybridization. values of greater than 15% were considered positive, and values of less than 5% were negative. cultures having hybridization value ... | 1989 | 2915018 |
| production and characterization of murine monoclonal antibodies to histoplasma capsulatum yeast cell antigens. | four monoclonal antibodies (mabs) were produced by immunizing mice with a disrupted yeast cell homogenate of histoplasma capsulatum. mabs 1 and 2 reacted only with the yeast cell antigens of h. capsulatum and blastomyces dermatitidis, whereas mabs 3 and 4 showed broader cross-reactivity. mab 3 cross-reacted with b. dermatitidis, paracoccidioides brasiliensis, sporothrix schenckii, and candida albicans, and mab 4 cross-reacted with b. dermatitidis, c. albicans, coccidioides immitis, aspergillus f ... | 1989 | 2917790 |
| modern management of cervical scrofula. | from 1970 to 1984, 32 patients were treated for tuberculous (tb) masses, which measured 1.2-5.5 cm. the posterior triangle was involved in 19 patients, anterior triangle in 7, and more than one triangle in 6 (3 bilateral). a superficial node was excised for diagnosis. acid-fast bacilli were identified on the smear of 18 patients. caseation necrosis was present in 27 specimens, sarcoidosis in four, nonspecific lymphadenitis in one; all cultures grew mycobacterium tuberculosis. a chest x-ray showe ... | 1989 | 2921112 |
| experimental allergic encephalomyelitis in guinea pig: variability of response to intradermal emulsion injection. | different forms of experimental allergic encephalomyelitis were obtained in 4 groups of guinea pigs: 7 adult hartley guinea pigs (group i), 12 adults of the same strain (group ii), 6 juvenile strain 2 guinea pigs (group iii) and 6 juvenile strain 13 animals (group iv), by the injection of emulsions. groups i and ii received emulsions containing 250 mg and 500 mg respectively of fresh isologous spinal cord tissue, complete freund adjuvant (cfa) and saline solution while groups iii and iv received ... | 1989 | 2925344 |
| [development and practical introduction of measures for improving the microbiological examination of tuberculosis patients]. | | 1985 | 2932733 |
| [organization of the microbiological examination of tuberculosis patients in the rsfsr]. | | 1985 | 2932734 |
| in-vitro activity of ciprofloxacin against clinical isolates of mycobacteria resistant to antimycobacterial drugs. | the activity of ciprofloxacin against 42 clinical isolates of mycobacteria was studied in vitro by the 1% standard proportion method on lowenstein-jensen medium. ciprofloxacin was found active against all strains of mycobacterium tuberculosis sensitive to streptomycin, isoniazid, ethambutol and rifampicin. the mic of ciprofloxacin was 3.2 mg/l. this concentration of ciprofloxacin was sufficient to inhibit almost all strains showing intermediate sensitivity or resistance to one or more of the abo ... | 1985 | 2933382 |
| in-vitro susceptibility of mycobacteria to ciprofloxacin. | two hundred and seventy-six strains of mycobacteria were tested for susceptibility to ciprofloxacin (bay 0 9867), a 4-quinolone antimicrobial agent. most strains of mycobacterium tuberculosis, myco. fortuitum, myco. kansasii, myco. marinum and myco. xenopi were sensitive to minimum inhibitory concentrations (mics) of 0.78-1.56 mg/l, equivalent to resistance ratios of 1 or 2. most strains of myco. avium-intracellulare and myco. chelonei required mics of 12.5 mg/l or more, giving resistance ratios ... | 1985 | 2934361 |
| generation of cytotoxic t lymphocytes from thymocyte precursors to trinitrophenyl-modified self antigens. ii. establishment of a t cell hybrid clone constitutively producing killer-helper factor(s) (khf) and functional analysis of released khf. | t cell hybridoma lines were constructed by fusion of mycobacterium tuberculosis-primed and boosted balb/c t cells with the akr-derived t lymphoma cell line bw5147. certain of the hybridomas prepared in this manner secreted constitutively into their culture supernatants biologically active molecules that displayed precursors of cytotoxic t cell activating properties characteristic of killer-helper factor (khf). cell surface analysis revealed that the hybridomas were indeed somatic cell hybrids be ... | 1986 | 2939132 |
| [value of different methods of detecting tuberculosis of the respiratory organs in adults]. | | 1986 | 2939448 |
| comparative in vitro activities of ciprofloxacin and other 4-quinolones against mycobacterium tuberculosis and mycobacterium intracellulare. | the in vitro activity of ciprofloxacin, ofloxacin, amifloxacin, and norfloxacin against 22 clinical isolates of mycobacterium tuberculosis was evaluated by agar dilution. the mics for 90% of the isolates of ciprofloxacin and ofloxacin were 0.5 and 1 microgram/ml, respectively. amifloxacin and norfloxacin were less active. the mics for 90% of the isolates of ciprofloxacin and ofloxacin against 20 clinical isolates of mycobacterium intracellulare were determined by agar dilution to be 2 and 8 micr ... | 1986 | 2940969 |
| [quality of sputum bacilloscopy in the laboratory network of argentina]. | | 1986 | 2941032 |
| immune response gene control of lymphocyte proliferation induced by acetylcholine receptor-specific helper factor derived from lymphocytes of myasthenic mice. | the role of lymphokines secreted by acetylcholine receptor (achr)-reactive lymphocytes in the regulation of an autoimmune response to achr has not been studied in the human or murine model of myasthenia gravis. we investigated whether achr-immune lymphocytes derived from mice with experimental autoimmune myasthenia gravis (eamg) can produce an achr-specific, genetically controlled soluble factor with biologic activity. achr-reactive lymphocytes of mice with eamg secreted an achr-specific helper ... | 1986 | 2943805 |
| aspects of mycobacterial response to beryllate ions in vitro. | comparative growth measurements of the mycobacterial species mycobacterium phlei and mycobacterium tuberculosis were carried out with liquid cultures supplemented with oxalatoberyllate ions in the concentration range 0-120 microm be. for both species, the effect of beryllium on specific growth parameters can be represented by a be concentration-dependent, exponential expression. evidence for beryllium binding to cellular phospholipids (approximately 15% of be uptake) is provided by 31p nmr spect ... | 1986 | 2946115 |
| [optimized methods of bacteriologic research on tuberculosis and the characteristics of its causative agent]. | | 1986 | 2947235 |
| [role of the bacteriological examination method in the dispensary care of persons at increased risk for tuberculosis]. | | 1986 | 2947236 |
| occupational health for hospital workers. | hospitals present a variety of infectious, chemical and physical hazards to their workers. important pathogens include aids, hepatitis b and rubella viruses and mycobacterium tuberculosis. ethylene oxide is probably the greatest chemical hazard, but anesthetic gases, antineoplastic drugs and skin irritants or sensitizers are significant hazards as well. hospitals also have poor safety records. a well-organized occupational health service that includes a physician, or a nurse with medical backup, ... | 1987 | 2949570 |
| possible role of helper and cytolytic t cells in mycobacterial infections. | | 1986 | 2952852 |
| comparative in vitro activity of the new macrolide a-56268 against mycobacteria. | | 1987 | 2959472 |
| [primary and acquired resistance in m. tuberculosis to antitubercular agents in the central bohemian region 1981-1985]. | | 1987 | 2961472 |
| [methodological letter on current clinical and therapeutic trends in ocular tuberculosis (i)]. | | 1987 | 2962246 |
| induction by an immunogenic immunomodulating agent of nonspecific t cell suppression of lymphocyte responsiveness in mlr but not of antibody production. | spleen cells derived from balb/c mice that had been repeatedly immunized with the methanol extraction residue (mer) fraction of tubercle bacilli exhibited a depressed capacity to act as responder cells in allogeneic and syngeneic mixed lymphocyte reactions (mlr). previously reported studies revealed that such spleen cells are also defective in the in vitro generation of antibodies. in order to determine the nature of the cells responsible for the depressed mlr reactivity, purified populations of ... | 1988 | 2965973 |
| [isolation of mycobacterium tuberculosis hominis from hemocultures in acquired immunodeficiency syndrome]. | | 1988 | 2966943 |
| [retrospective bacteriological study of mycobacterial infections in patients with acquired immunodeficiency syndrome]. | the main species of mycobacteria isolated in 62 of the 316 acquired immunodeficiency syndrome patients admitted to the claude bernard hospital, paris, between january, 1983 and october, 1986 were studied retrospectively according to their site of isolation and their pathogenic role. mycobacterium tuberculosis was isolated in 19 cases (from pulmonary specimens in 17 cases); this species was present in 59 percent of our african patients as against 20 percent of our european patients. m. avium intr ... | 1988 | 2967954 |
| efficacy of a cell-mediated reaction to the purified protein derivative of tuberculin in the disposal of mycobacterium leprae from human skin. | the purpose of this study was to evaluate the effects of a delayed-type cell-mediated immune response to mycobacterium tuberculosis antigen on the mycobacterium leprae load in the skin of leprosy patients. twelve patients with the lepromatous form of leprosy have been injected intradermally with 5 units of the purified protein derivative of tuberculin (ppd). ten individuals responded with areas of induration ranging from 12 to 21 mm in diameter, and two were unresponsive (less than 10 mm). twent ... | 1988 | 2969108 |
| [tuberculosis in patients infected with the human immunodeficiency virus. 30 cases]. | between may, 1983 and september, 1987, 8 per cent of the patients hospitalised for an hiv infection (i.e. 30 patients, 20 with an arc and 10 with aids) had tuberculosis. the percentage of patients originating from central africa or haiti was important (23 per cent). tuberculosis was thoracic (76 per cent) and/or extrathoracic (63 per cent). the main organs involved were the lungs (n = 21), the mediastinal lymph nodes (n = 9), the superficial lymph nodes (n = 9), and the liver (n = 8). the pulmon ... | 1988 | 2971192 |
| [the nature of bacterial shedding and its effect on treatment outcome in newly detected patients with destructive pulmonary tuberculosis]. | | 1988 | 2974156 |