| the effect of antimicrobial therapy on mixed infections with bacteroides species. is eradication of the anaerobes important? | antimicrobial agents were used alone or in combinations in order to explore their effect on mixed aerobic-anaerobic infections. subcutaneous abscesses were induced in mice by single and mixed infections of bacteroides fragilis, bacteroides melaninogenicus, staphylococcus aureus, streptococcus pyogenes, streptococcus faecalis, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa. the infected animals were treated for 5 days with spiramycin, gentamicin or metronidazole alone, or metr ... | 1991 | 2002230 |
| urothelial hyperplasia and neoplasia. iii. detection of nitrosamine production with different bacterial genera in chronic urinary tract infections of rats. | various agents have been implicated in inducing urothelial cancer. although drugs, occupational and environmental carcinogens are more widely accepted as playing a major role as urothelial carcinogens, several investigations suggest that bacteria may play a role. the mechanism of how bacteria may interact with the host to augment the development of urothelial carcinoma is not well understood. clinically, investigators have linked the development of infection, urinary stones and indwelling cathet ... | 1991 | 2005721 |
| clostridium perfringens or klebsiella pneumoniae as the cause of a food-borne outbreak. | | 1991 | 2007653 |
| two malt binding sites in direct repeat. a structural motif involved in the activation of all the promoters of the maltose regulons in escherichia coli and klebsiella pneumoniae. | the maltose regulons of escherichia coli and klebsiella pneumoniae are very similar, comprising three operons that code for the proteins required for the utilization of maltodextrins as a carbon source. the maltose regulon of k. pneumoniae contains two additional operons, pulab and pulc-o, which allow the use of starch as a carbon source. the promoters of all of these operons are strictly controlled by the activator protein malt. in this paper, we report a detailed study of the structure and the ... | 1991 | 2010912 |
| gram-negative bacterial infections of the mammary gland in cows. | naturally acquired gram-negative bacterial intramammary infections (n = 160) were studied in 99 cows over a 2-year period. escherichia coli, klebsiella spp, serratia spp, enterobacter spp, and unidentified gram-negative bacteria were isolated from 28.8, 39.4, 9.4, 5.0, and 11.2%, respectively, of infected mammary glands. a majority (61%) of intramammary infections were first detected during the nonlactating period. gram-negative bacteria isolated during the first half of the nonlactating period ... | 1991 | 2012328 |
| ceftibuten and bactericidal kinetics. comparative in vitro activity against enterobacteriaceae producing extended spectrum beta-lactamases. | ceftibuten, compared to cefixime, cefetamet, cefpodoxime, loracarbef, cefprozil, cefuroxime, cefaclor, and cefadroxil, was the most active oral cephalosporin derivative against enterobacteriaceae producing plasmid-encoded broad spectrum beta-lactamases. in a pharmacodynamic model, ceftibuten was bactericidal for haemophilus influenzae and streptococcus pneumoniae at concentrations simulating human serum levels following 200 mg, p.o., b.i.d. | 1991 | 2013215 |
| [distribution and antibiotic resistance of aerobic bacteria isolated from infected wounds caused by burns]. | distribution according to species and genus and resistance to antibacterial agents was studied with 420 bacterial isolates cultured from the wound excretion of 282 burned-infected patients. of the isolates 68.2% was gram-positive and 31.8% gram-negative. this latter high rate may be due to fecal infection as 21% of the burned patients was between the age of 0-4 years. of the total isolates 38.3% was staphylococcus aureus, 16.7% coagulase-negative staphylococcus, 10.7% pseudomonas strain, 6.9% es ... | 1991 | 2014125 |
| intensive care management of community-acquired klebsiella pneumoniae. | the clinical features of 18 patients with klebsiella pneumoniae requiring intensive care unit (icu) management are presented. all patients required ventilatory support; 17 were given constant positive pressure ventilation and 10 required greater than 10 cm positive end expiratory pressure. the clinical picture was characteristic: pre-existing medical disease, clinical features of severe pneumonia and copious purulent bronchial secretions, gram--ve organisms on gram's stain and lobar consolidatio ... | 1991 | 2014354 |
| antibacterial activities in vitro and in vivo and pharmacokinetics of cefquinome (hr 111v), a new broad-spectrum cephalosporin. | cefquinome is a new injectable aminothiazolyl cephalosporin derivative. it is stable against chromosomally and plasmid-encoded beta-lactamases and has a broad antibacterial spectrum. staphylococcus aureus, streptococci, pseudomonas aeruginosa, and members of the family enterobacteriaceae (escherichia coli, salmonella spp., klebsiella spp., enterobacter spp., citrobacter spp., and serratia marcescens) are inhibited at low concentrations. cefquinome is also active against many strains of methicill ... | 1991 | 2014969 |
| human monoclonal antibodies specific for capsular polysaccharides of klebsiella recognize clusters of multiple serotypes. | we report the generation and the characterization of a set of human monoclonal antibodies (hmab) specific for gram-negative bacteria of klebsiella pneumoniae. the eight human hybridomas secrete either igm kappa, iga1 kappa, or iga2 kappa antibodies. one hmab binds bacteria of only one serotype. five hmab recognize non-overlapping clusters of 2, 3, or 10 different serotypes. the remaining two hmab both recognize three serotypes. two serotypes are recognized by both hmab, and in addition both hmab ... | 1991 | 2016541 |
| severe sepsis following extracorporeal shock wave lithotripsy. | a patient with life-threatening sepsis following extracorporeal shock wave lithotripsy (eswl*) is described. the urosepsis was complicated by meningitis and diffuse metastatic endophthalmitis necessitating enucleation of the right eye. retinal detachment occurred in the left eye following focal endophthalmitis and the operation yielded a poor functional result. the patient was discharged from the hospital 3 months after the eswl therapy, free of urinary stones. the etiology, treatment and prophy ... | 1991 | 2016789 |
| characteristics of group a streptococcal bacteremia in patients at the san bernardino county medical center. | on the basis of a 12-year study of 58 patients at our medical center who had group a streptococcal bacteremia, we found that this disease is not uncommon when compared with other varieties of bacteremia. it occurs with about one-half of the frequency of bacteremias due to klebsiella species and one-eighth of that of bacteremias due to staphylococcus aureus. the infection is infrequently nosocomial (3.5% of cases) and the predominant causes are cutaneous or subcutaneous infections (72% of cases). ... | 1991 | 2017638 |
| lectinophagocytosis of encapsulated klebsiella pneumoniae mediated by surface lectins of guinea pig alveolar macrophages and human monocyte-derived macrophages. | macrophages express a mannose/n-acetylglucosamine-specific lectin which serves as a receptor for nonopsonic phagocytosis of mannose-coated particles. we have examined the binding to guinea pig alveolar macrophages in a serum-free medium of 16 klebsiella pneumoniae serotypes and of the capsular polysaccharides isolated from 7 of these serotypes. only five polysaccharides containing the repeating sequence man alpha 2/3man or l-rha alpha 2/3-l-rha bound to the macrophages. of the 11 bacterial serot ... | 1991 | 2019437 |
| bilateral endogenous endophthalmitis in a patient with diabetes and renal papillary necrosis. | | 1991 | 2021183 |
| klebsiella pneumoniae liver abscess with septic endophthalmitis. the role of computed tomography. | klebsiella pneumoniae liver abscess associated with septic endophthalmitis is a rare observation. to our knowledge, only 10 cases have been reported in the international literature. we report a further case of complication occurring in an adult woman with hypothyroidism, and comment on the role of ct for the work-up. | 1991 | 2022607 |
| the n-terminal and c-terminal portions of nifv are encoded by two different genes in clostridium pasteurianum. | the nifv gene products from azotobacter vinelandii and klebsiella pneumoniae share a high level of primary sequence identity and are proposed to catalyze the synthesis of homocitrate. while searching for potential nif (nitrogen fixation) genes within the genomic region located downstream from the nifn-b gene of clostridium pasteurianum, we observed two open reading frames (orfs) whose deduced amino acid sequences exhibit nonoverlapping sequence identity to different portions of the nifv gene pro ... | 1991 | 2022611 |
| tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian tracheal mucosa: peptide isolation and cloning of a cdna. | extracts of the bovine tracheal mucosa have an abundant peptide with potent antimicrobial activity. the 38-amino acid peptide, which we have named tracheal antimicrobial peptide (tap), was isolated by a sequential use of size-exclusion, ion-exchange, and reverse-phase chromatographic fractionations using antimicrobial activity as a functional assay. the yield was approximately 2 micrograms/g of wet mucosa. the complete peptide sequence was determined by a combination of peptide and cdna analysis ... | 1991 | 2023943 |
| rapid diagnosis of legionella infection by a nonisotopic in situ hybridization method. | the authors report a nonradioactive adaptation of dna hybridization technology for the direct detection of legionella organisms in situ in routinely processed histologic specimens. the probe used consisted of synthetic oligodeoxynucleotides, complementary to the ribosomal rna of all clinically relevant legionella species, labeled with biotinylated dutp at their 3' ends. by in situ dna hybridization and detection with an avidin-alkaline phosphatase complex. legionella was visualized by light micr ... | 1991 | 2024627 |
| in vitro activity and beta-lactamase stability of gr69153, a new long-acting cephalosporin. | gr69153, a new parenteral cephalosporin, inhibited 90% of escherichia coli, klebsiella oxytoca, proteus mirabilis, citrobacter diversus, shigellae, and salmonellae at less than 0.25 micrograms/ml (mic90). it had activity comparable to those of ceftazidime, cefpirome, cefepime, and e-1040. against cephalosporinase-producing enterobacter cloacae, citrobacter freundii, and serratia marcescens, mics ranged from 0.12 to greater than 32 micrograms/ml, and cefpirome and cefepime were the most active ag ... | 1991 | 2024959 |
| aztreonam for treating meningitis caused by gram-negative rods. | 10 patients with meningitis due to unusual gram-negative organisms (pseudomonas, proteus, salmonella and klebsiella) were effectively treated with aztreonam. a detailed history and a thorough physical examination combined with careful laboratory testing resulted in accurate diagnosis and cure of all patients. | 1991 | 2028226 |
| hemagglutinins of klebsiella pneumoniae and k. oxytoca isolated from different sources. | 482 klebsiella pneumoniae and k. oxytoca strains isolated from different sources (human clinical material, feces of healthy subjects, sewage) were investigated for expression of ms-(mannose-sensitive) and mr/k-(mannose-resistant, klebsiella-like) hemagglutinins. regardless of the source of isolation, the majority of k. pneumoniae strains induced mannose-sensitive hemagglutination, but very few k. oxytoca isolates produced this type of hemagglutinin. in both species, most strains induced mr/k-hem ... | 1991 | 2029324 |
| purification and characterization of a catalase-peroxidase and a typical catalase from the bacterium klebsiella pneumoniae. | the bacterium klebsiella pneumoniae synthesizes three different types of catalase: a catalase-peroxidase, a typical catalase and an atypical catalase, designated kpcp, kpt and kpa, respectively (goldberg, i. and hochman, a. (1989) arch. biochem. biophys. 268, 124-128). kpcp, but not the other two enzymes, in addition to the catalatic activity, catalyzes peroxidatic activities with artificial electron donors, as well as with nadh and nadph. both kpcp and kpt are tetramers, with heme ix as a prost ... | 1991 | 2029529 |
| [chronic pneumonia caused by klebsiella pneumoniae and escherichia coli acquired in the community]. | | 1991 | 2029564 |
| in vitro activities of antibacterial agents against clinical isolates of escherichia coli and klebsiella species from intensive care units. | the susceptibility of 293 cultures of escherichia coli and 160 cultures of klebsiella species isolated consecutively from patients in intensive care units at 25 new york area hospitals to four antibiotic agents was determined. susceptibility testing was performed with the reference agar dilution and broth microdilution procedures. at the minimum inhibitory concentration (mic) breakpoints (specified in the prescribing information) of less than or equal to 4 micrograms/ml for imipenem, less than o ... | 1991 | 2029725 |
| increased urinary nitrosamine excretion in paraplegic patients. | an increased risk of bladder cancer is a recognized complication in spine-injured patients undergoing long-term urethral catheterization to preserve renal function. aerobic cultures from 28 of 30 paraplegic patients showed complex bacterial flora containing nitrate-reducing organisms (escherichia coli, proteus and klebsiella spp.). urine samples from 29 paraplegic patients were also found to contain volatile nitrosamines. mean n-nitrosamine excretion levels were 0.65 +/- 0.69 micrograms/day n-ni ... | 1991 | 2029761 |
| influenza a and the virus associated haemophagocytic syndrome: cluster of three cases in children with acute leukaemia. | at the height of the united kingdom influenza a epidemic in december 1989, three children receiving treatment for non-t cell acute leukaemia developed pancytopenia with concomitant influenza a infection. bone marrow histology showed prominent marrow erythrophagocytosis by morphologically mature histiocytes, consistent with the picture of virus associated haemophagocytic syndrome (vahs). in two cases there was an initial spontaneous recovery, though recurrence of vahs developed in one case in ass ... | 1991 | 2030147 |
| pathogenicity of propionibacterium acnes in mixed infections with facultative bacteria. | single and mixed infections with 11 clinical isolates of propionibacterium acnes and three facultative bacteria (staphylococcus aureus, escherichia coli and klebsiella pneumoniae) were studied in a subcutaneous abscess model in mice. abscesses were induced by pure cultures of six of 11 strains of p. acnes and by the three facultative bacteria. the abscesses produced by each of the six "virulent" p. acnes isolates mixed with s. aureus, e. coli or k. pneumoniae were larger than those induced by th ... | 1991 | 2030499 |
| pyridoxal phosphate-dependent histidine decarboxylases. cloning, sequencing, and expression of genes from klebsiella planticola and enterobacter aerogenes and properties of the overexpressed enzymes. | the hdc genes encoding the inducible pyridoxal-p-dependent histidine decarboxylase (hisdcase) of klebsiella planticola and enterobacter aerogenes were isolated, sequenced, and expressed in escherichia coli under control of the lac promoter, and the overproduced enzymes were purified to homogeneity from the recombinant host. formation of inclusion bodies during synthesis of the e. aerogenes enzyme was avoided by cooling the culture and inducing at 25 degrees c. the cloned enzymes were produced in ... | 1991 | 2033044 |
| molecular characterization of a new plasmid-encoded shv-type beta-lactamase (shv-2 variant) conferring high-level cefotaxime resistance upon klebsiella pneumoniae. | between 1986 and 1988, multiresistant klebsiella pneumoniae strains exhibiting high-level cefotaxime resistance were isolated from patient specimens particularly of the intensive care units of the aachen technical university hospital. the resistance gene responsible was shown to be encoded on a conjugative 66 kb plasmid designated pzmp1. the mic values for cefotaxime of the original isolates and the transconjugants were greater than 128 mg l-1 and 64 mg l-1, respectively. isoelectric focusing of ... | 1991 | 2033379 |
| biosynthesis of klebsiella k2 capsular polysaccharide in escherichia coli hb101 requires the functions of rmpa and the chromosomal cps gene cluster of the virulent strain klebsiella pneumoniae chedid (o1:k2). | the genes determining the biosynthesis of type 2 (k2) capsular polysaccharide [3----beta glc1,4----beta man(1,3----beta glcua) 1,4----alpha glc1----] of klebsiella pneumoniae chedid (o1:k2), which is highly virulent for mice, were cloned and introduced into escherichia coli hb101 and into four noncapsulated mutants derived from k. pneumoniae reference strains of k1, k7, k9, and k28. the recombinant plasmid pcps7b06 carried 23 kb of a chromosomal dna fragment of strain chedid and encoded a part o ... | 1991 | 2037364 |
| antimicrobial effect of clindamycin in combination with aztreonam or aminoglycosides against klebsiella spp. | the antimicrobial effect of clindamycin combined with aztreonam or an aminoglycoside (gentamicin, tobramycin or amikacin) was studied against 84 strains of klebsiella pneumoniae and 18 strains of k. oxytoca with an agar dilution technique. clindamycin concentrations of 1-20 mg/l and an inoculum of 10(4) cuf/spot were used. anaerobic incubation of agar plates was associated with an increase in the mic of aminoglycosides and no change or a decrease in the mic of aztreonam. lower concentrations of ... | 1991 | 2037536 |
| reactivity of the essential thiol of klebsiella aerogenes urease. effect of ph and ligands on thiol modification. | the kinetics of klebsiella aerogenes urease inactivation by disulfide and alkylating agents was examined and found to follow pseudo-first-order kinetics. reactivity of the essential thiol is affected by the presence of substrate and competitive inhibitors, consistent with a cysteine located proximal to the active site. in contrast to the results observed with other reagents, the rate of activity loss in the presence of 5,5'-dithiobis(2-nitrobenzoic acid) (dtnb) saturated at high reagent concentr ... | 1991 | 2037578 |
| antibiotic-bonded ptfe vascular grafts: the effect of silver antibiotic on bioactivity following implantation. | use of an infection-resistant vascular prosthesis has appeal for grafting in sites of potential contamination or to replace graft segments involved in low-grade infections caused by coagulase-negative staphylococci (cns). in this study, antibiotic retention on polytetrafluoroethylene (ptfe) grafts prepared using three antibiotic-bonding methods was compared following implantation into the arterial circulation. ciprofloxacin or silver-ciprofloxacin was bonded to ptfe surfaces by surfactant-mediat ... | 1991 | 2038181 |
| bacterial colonisation of humidifier attachments on oxygen concentrators prescribed for long term oxygen therapy: a district review. | a microbiological survey was undertaken on the eight patients in the liverpool district who have a humidifier attachment on their oxygen concentrator. all but one of the humidifiers were contaminated with potentially pathogenic bacteria. | 1991 | 2038734 |
| national survey of clinical and subclinical mastitis in jamaican dairy herds, 1985-1986. | between april 1985 and august 1986, 89 jamaican dairy herds with 10 or more cows were visited, 1,645 lactating cows were examined using the cmt test and 254 composite milk samples collected for bacteriological examination. widespread management faults were noted, especially of milking machine usage and maintenance and the abuse of antibiotics. fifty-six per cent of all quarters were found to have cmt scores of one or higher, 0.8% showed clinical mastitis and 3.2% were blind. the most common bact ... | 1991 | 2038766 |
| comparison of the effects of aztreonam and tigemonam against escherichia coli and klebsiella pneumoniae in vitro and in vivo. | a study was performed to investigate the pharmacodynamics of aztreonam and tigemonam against escherichia coli and klebsiella pneumoniae in vitro and in vivo. the in vitro concentration-effect relationships were determined in short-term growth experiments. the in vivo dose-effect relationships were determined in an experimental thigh muscle infection in irradiated mice. in this model, e. coli was injected into one thigh muscle and k. pneumoniae was injected into the other. throughout these experi ... | 1991 | 2039191 |
| activity of temafloxacin against respiratory pathogens. | the activity of the quinolone temafloxacin against respiratory pathogens was compared with those of ciprofloxacin and ofloxacin. mics for 90% of strains tested indicated that temafloxacin was at least two- to fourfold more potent than the other two quinolones against staphylococcus aureus, streptococcus pneumoniae, and legionella pneumophila. temafloxacin had potency equal to that of ciprofloxacin and was twofold more active than ofloxacin against streptococcus pyogenes. moraxella catarrhalis, a ... | 1991 | 2039192 |
| viable but nonculturable bacteria in drinking water. | klebsiella pneumoniae, enterobacter aerogenes, agrobacterium tumefaciens, streptococcus faecalis, micrococcus flavus, bacillus subtilis, and pseudomonas strains l2 and 719 were tested for the ability to grow and maintain viability in drinking water. microcosms were employed in the study to monitor growth and survival by plate counts, acridine orange direct counts (aodc), and direct viable counts (dvc). plate counts dropped below the detection limit within 7 days for all strains except those of b ... | 1991 | 2039237 |
| proteolytic interconversion and n-terminal sequences of the citrobacter diversus major beta-lactamases. | the n-terminal sequences of the two major beta-lactamases produced by citrobacter diversus differed only by the absence of the first residue in form ii and the loss of five amino acid residues at the c-terminal end. limited proteolysis of the homogeneous form i protein yielded a variety of enzymatically active products. in the major product obtained after the action of papain, the first three n-terminal residues of form i had been cleaved, whereas at the c-terminal end the treated enzyme lacked ... | 1991 | 2039443 |
| [antibacterial activity of ciprofloxacin against fresh clinical isolates from superficial suppurative foci]. | the minimum inhibitory concentrations (mics) of 5 drugs (ciprofloxacin (cpfx), and 4 drugs used as standard) were determined to investigate antibacterial potencies of cpfx against bacterial strains isolated in 1989 from superficial suppurative foci. the clinical isolates tested included 375 strains from 11 aerobic bacterial species, and 50 strains from 2 anerobic bacterial genera (group) for a total of 425 isolates. interpreting mic level distributions of these drugs as the expression of antibac ... | 1991 | 2041153 |
| [pharmacokinetic and clinical studies on cefpirome in pediatrics]. | cefpirome (cpr, hr 810), a new parenteral cephalosporin antibiotic, was studied for its pharmacokinetics, bacteriological and clinical effects in the field of pediatrics. 1. cpr was very active against staphylococcus aureus, staphylococcus epidermidis, coagulase-negative staphylococci, streptococcus pneumoniae among gram-positive cocci. antibacterial activities of cpr were also strong against branhamella catarrhalis, haemophilus influenzae, escherichia coli, salmonella sp., klebsiella oxytoca, e ... | 1991 | 2041158 |
| [pharmacokinetical and clinical study of cefpirome in children]. | this study describes the pharmacokinetic characteristics and clinical usefulness of cefpirome (cpr) in children. mean half-lives of 20 mg/kg and 40 mg/kg of cpr injected intravenously in one shot were 1.18 and 1.34 hours, respectively, and their mean recovery rates into urine were 69.8 and 72.2%, respectively. minimum inhibitory concentrations of cpr against staphylococcus aureus, streptococcus pneumoniae, klebsiella pneumoniae, escherichia coli and haemophilus influenzae were the same as or low ... | 1991 | 2041162 |
| the general protein-export pathway is directly required for extracellular pullulanase secretion in escherichia coli k12. | pullulanase is an extracellular, cell surface-anchored lipoprotein produced by gram-negative bacteria belonging to the genus klebsiella. its correct localization in recombinant escherichia coli requires the products of 14 genes that are linked to the enzyme structural gene in the klebsiella chromosome. in addition, we show here that six sec genes (seca, secb, secd, sece, secf and secy) are all required for processing of the prepullulanase signal peptide to occur. this implies that pullulanase cr ... | 1991 | 2041472 |
| influence of a mutation in the putative nucleotide binding site of the nitrogen regulatory protein ntrc on its positive control function. | a mutation, serine 170 to alanine, in the proposed atp binding site of the activator protein ntrc prevents transcriptional activation at sigma 54-dependent promoters both in vivo and in vitro. the rate of phosphorylation of the mutant protein by ntrb and the stability of mutant ntrc-phosphate were similar to those of wild-type ntrc. the phosphorylated mutant protein shows only a slight decrease in affinity (around 2-fold) for tandem ntrc binding sites in the klebsiella pneumoniae nifl promoter s ... | 1991 | 2041769 |
| bacterial antibodies in ankylosing spondylitis. | antibodies to salmonellae, yersiniae, campylobacter jejuni, borrelia burgdorferi, klebsiella pneumoniae, escherichia coli, proteus mirabilis and chlamydia trachomatis were measured by elisa in the sera of 99 patients with ankylosing spondylitis. increased prevalence of iga and igg class antibodies against k. pneumoniae and of iga class against e. coli was observed in ankylosing spondylitis. no clear correlation between the disease activity and occurrence of antibodies was revealed. the results a ... | 1991 | 2044228 |
| identification of a promoter dependent on nifa and sigma 54 upstream of nifh in azospirillum lipoferum. | southern hybridization experiments strongly indicate that the regulatory region of the azospirillum lipoferum nifh gene is located on a cloned 1.1 kb bamhi-xhoi restriction fragment. by cloning this fragment into a promoter-probe plasmid in escherichia coli, a promoter was identified oriented towards the nifh gene. using a set of several bacterial strains and plasmids, both nifa and the alternative sigma factor, sigma 54, from klebsiella pneumoniae were shown to be required for the induction of ... | 1991 | 2046661 |
| oral mucosal soft tissue necrosis caused by superinfection. report of three cases. | three patients in whom flap necrosis developed after oral surgeries and antibiotic therapies were studied microbiologically by routine aerobic and anaerobic methods. in all cases the bacteria enterobacter cloacae, klebsiella oxytoca, escherichia coli, and coagulase-negative penicillinase-producing staphylococci were resistant to the antibiotics used. these bacteria are frequent microorganisms of superinfection and were not found after the necrotic tissues had repaired. | 1991 | 2047093 |
| prevention of bacterial infection and lps-induced lethality by interleukin-1 alpha in mice. | in order to make clear the characteristic biological properties of il-1 alpha, its protective capabilities on bacteria-induced and lps-induced lethality were compared with other brms in balb/c mice. pretreatment with il-1 alpha (i.p., s.c.) or ok-432 (i.p.), could protect mice from the lethality of a k. pneumoniae infection (i.p.), and pretreatment with il-1 alpha or tnf alpha could protect mice from lps (i.p.) lethality. il-1 alpha could protect mice from both bacteria- and lps-induced lethalit ... | 1991 | 2048119 |
| open randomized controlled parallel study of ofloxacin versus trimethoprim-sulfamethoxazole treatment of lower respiratory tract and urinary infections. | a total of 60 patients with lower respiratory tract or urinary tract infections were enrolled in an open, randomized, controlled, parallel study comparing 300 mg ofloxacin (ofx) b.i.d. with trimethoprim + sulfamethoxazole (tmp 800 mg + smx 160 mg), 1 tablet, b.i.d. the signs and symptoms of low respiratory tract infection were cured in 12 patients (80%) of the ofx group and improved in 2 other patients (13%); at the end of therapy, the 2 germs that persisted were streptococcus pneumoniae and bra ... | 1991 | 2049964 |
| a skin suction blister model in hairless rats: application to the study of anti-inflammatory and immunomodulatory drugs. | a suction blister model was developed in the hairless rat, in order to study the effects of various agents on the migration of polymorphonuclear leukocytes (pmn). a standardized abrasion, a suction blister, was formed by applying negative pressure to the skin and then separating the epidermis from the dermis. a migration chamber containing serum as the chemoattractant was placed over the wound. after 6 h of migration, the cells in the chamber were harvested, counted and identified. we evaluated ... | 1991 | 2050441 |
| activity of human extravascular temocillin concentrations simulated in an in-vitro kinetic model against beta-lactamase producing gram-negative bacilli. | an in-vitro kinetic model was used to demonstrate the bactericidal activity of concentrations of temocillin achieved in extravascular body fluids following a 1 g intravenous dosage in man. concentrations of temocillin attainable in peripheral lymph were bactericidal for ampicillin-resistant strains of escherichia coli, enterobacter cloacae and klebsiella oxytoca. likewise, concentrations of temocillin achieved in human blister fluid following a 12-hourly dosage regimen were bactericidal for cult ... | 1991 | 2050587 |
| a weak adaptive response to alkylation damage in salmonella typhimurium. | an efficient adaptive response to alkylation damage was observed in several enterobacterial species, including klebsiella aerogenes, shigella sonnei, shigella boydii, escherichia alkalescens, escherichia hermanii, and escherichia fergusonii. increased o6-methylguanine-dna and methylphosphotriester-dna methyltransferase activities correlated with the induction of a 39-kda protein recognized by monoclonal antibodies raised against the escherichia coli ada protein. induced methyltransferase activit ... | 1991 | 2050626 |
| the bactericidal power of the blood and plasma of patients with burns. | patients with burns are unusually susceptible to bacterial infections, but so far there is no satisfactory explanation for this lack of resistance. since resistance to infection involves many different mechanisms, examination of individual components of the immune system may not sufficiently explain the underlying reasons for increased susceptibility. the use of whole blood for antibacterial tests has the advantage that all the immune systems present in that fluid compartment can take part in th ... | 1991 | 2050718 |
| klebsiella pneumoniae urinary tract infection complicated by endophthalmitis, perinephric abscess, and ecthyma gangrenosum. | we have reported the case of an immunocompromised patient with a k pneumoniae bacteremia admitted with endophthalmitis. the source of the infection was an asymptomatic left renal calculus associated with a perinephric abscess. persistent bacteremia resulted in the development of ecthyma gangrenosum, which has not previously been associated with klebsiella spp infection. | 1991 | 2052978 |
| current issues in burn wound infections. | as we have emphasized, the diagnosis of burn wound infections in the high-risk burned child can be difficult and depends on a very high degree of suspicion and daily clinical evaluation of the burn wound site by consistent observers. appropriate precautions include meticulous hand-washing and the use of gloves when handling the wound site and prophylactic application of a topical antibacterial agent such as ssd cream. wound therapy should include routine vigorous surgical débridement. surveillan ... | 1991 | 2054118 |
| characterization of beta-lactam-resistant klebsiella oxytoca isolated in a neonatal intensive care unit. | the occurrence of klebsiella oxytoca resistant to ampicillin, piperacillin, aztreonam and cefuroxime in a neonatal intensive care unit, including two cases of septicemia, was shown to consist of a spread on three consecutive occasions caused by three different biochemical klebsiella oxytoca phenotypes. all isolates, except six surface isolates from one infant belonging to phenotype 1, were sensitive to cefotaxime (mic 0.5-4 mg/l) and ceftazidime (mic 0.25-1 mg/l). isolates of phenotypes 1 and 2 ... | 1991 | 2054170 |
| treatment with recombinant human tumor necrosis factor-alpha protects rats against the lethality, hypotension, and hypothermia of gram-negative sepsis. | tumor necrosis factor (tnf) is a peptide secreted by macrophages in response to endotoxin that can produce many of the changes seen in septic shock. after cecal ligation and puncture (clp) rats gradually develop tachycardia, hypotension, tachypnea, and hypothermia. at 5 h post-clp, rats have a peak in serum levels of endotoxin and 60% of rats have blood cultures that grow gram-negative rods (escherichia coli and klebsiella pneumonia). at 20 h post-clp all rats develop positive blood cultures. se ... | 1991 | 2056127 |
| management of anorectal/perineal infections caused by pseudomonas aeruginosa in children with malignant diseases. | the role of operation for anorectal infections associated with perineal gangrene and cellulitis in children with myelo-suppression from cancer chemotherapy is unclear. we evaluated anorectal/perineal infections caused by pseudomonas aeruginosa in 16 children with malignant diseases seen over 27 years. in 12 of 16 patients, leukemia was the underlying malignancy (all 10, aml 2), and in 13 of 16, severe neutropenia (absolute neutrophil count less than 500/mm3) was present at diagnosis. cultures of ... | 1991 | 2056413 |
| mixed acid fermentation of glucose as a mechanism of emphysematous urinary tract infection. | emphysematous pyelonephritis is a severe necrotizing infection that usually occurs in patients with diabetes mellitus or obstructive uropathy. although glucose fermentation has been considered as the main cause of gas production the actual mechanism remains controversial. compositions of gas samples from 2 patients with emphysematous pyelonephritis recently encountered were analyzed, and showed 15% hydrogen, 4.8% carbon dioxide, 60% nitrogen, 6.7% oxygen and some unknown gases in case 1, and 3.4 ... | 1991 | 2056576 |
| a new mouse model for autoimmune orchitis. | experimental autoimmune orchitis (eao) was induced in sma mice (h-2nondefined) by repeated injection at intervals of 30 days of syngeneic testis homogenate (th) together with klebsiella o3 lipopolysaccharide (ko3 lps) as a potent adjuvant. eao was not induced by repeated injection of th alone or ko3 lps alone. at 10 days after the secondary injection of th + ko3 lps, there was marked infiltration with neutrophils in the seminiferous tubules and in the interstitium of the testis accompanied by de ... | 1991 | 2056961 |
| effect of respiratory infection on tissue riboflavin and flavin enzymes in mice. | the effects of klebsiella pneumoniae infection on red blood cell and liver riboflavin levels and a few flavin enzymes were examined to verify our earlier hypothesis based on human experience that upper respiratory infections result in mobilization of riboflavin from tissues into blood, resulting in a rise in red blood cell riboflavin and an increased saturation of erythrocyte glutathione reductase (egr) with its coenzyme flavin adenine dinucleotide (fad; reduction in egr activation coefficient, ... | 1991 | 2058998 |
| vaginal infection and preterm labour. | to study the vaginal flora of women in preterm labour (ptl) and determine whether the presence of specific vaginal microflora is significantly associated with onset of ptl. | 1991 | 2059587 |
| bacteremia in adult diabetic patients. | to compare the microbiology, sources, complications, and outcome of bacteremia in diabetic and nondiabetic patients. | 1991 | 2060428 |
| a new family of bacterial regulatory proteins. | a new family of bacterial regulatory proteins has been identified by sequence similarity. the family contains the repressor of the bacillus subtilis gluconate operon (gntr), the regulators for histidine utilization in pseudomonas putida (hutcpp) and klebsiella aerogenes (hutcka), the repressor (fadr) of fatty acid degradation in escherichia coli, a regulator involved in the conjugal transfer of the broad host range plasmid pij101 (kora), and three proteins of unidentified function in e. coli (ge ... | 1991 | 2060763 |
| studies on cephalosporin antibiotics. iii. synthesis, antibacterial activity and oral absorption of new 3-(substituted-alkylthio)-7 beta-[(z)-2-(2-aminothiazol-4-yl)-2- (carboxymethoxyimino)acetamido]cephalosporins. | the synthesis, antibacterial activity and oral absorption in rats of new 7 beta-[(z)-2-(2-aminothiazol-4-yl)-2-(carboxymethoxyimino)ace tamido] cephalosporins (1) having various substituted-alkylthio groups at the c-3 position of the cephem nucleus are described. of these, the cephalosporins with a cyanomethylthio group (1d) and fluoroethylthio group (1p) at the c-3 position showed a potent in vitro antibacterial activity against gram-positive and gram-negative bacteria as well as good oral abso ... | 1991 | 2061193 |
| nucleotide sequence of rmpb, a klebsiella pneumoniae gene that positively controls, colanic biosynthesis in escherichia coli. | production of colanic acid in escherichia coli is regulated by two negative regulators, lon and rcsc, and by two positive regulators, rcsa and rcsb. two genes of klebsiella pneumoniae, rmpa and rmpb, have been shown to positively control colanic acid synthesis in e. coli. while colanic acid production is activated by rmpa only in a lon strain of e. coli, a plasmid carrying both rmpa and rmpb can stimulate colanic acid synthesis in a lon+ strain. in this work, we present the determination of the ... | 1991 | 2068379 |
| aztreonam versus gentamicin for short-term prophylaxis in biliary and gastric surgery. | short-term antibiotic prophylaxis was studied in 80 patients undergoing biliary or gastric surgery. the patients were randomized to receive 1 g of aztreonam or 80 mg of gentamicin intravenously 30 minutes before surgery and 8 and 16 hours after surgery. of samples taken from the abdominal cavity for bacteriologic study, 53% were culture positive. wound infections developed in two (4.5%) of 44 patients receiving aztreonam and in seven (19.4%) of 36 patients treated with gentamicin. staphylococcus ... | 1991 | 2068469 |
| treatment of gram-negative peritonitis with aztreonam in patients undergoing continuous ambulatory peritoneal dialysis. | in a multicenter open study, intraperitoneal aztreonam was used together with vancomycin, cloxacillin, or flucloxacillin for initial empiric treatment of peritonitis associated with continuous ambulatory peritoneal dialysis (capd). monotherapy with aztreonam was continued in 34 episodes of gram-negative peritonitis in 28 patients. the microorganisms isolated included escherichia coli, acinetobacter species, pseudomonas species, and klebsiella species. in three episodes, two organisms were cultur ... | 1991 | 2068475 |
| efficacy and safety of aztreonam in the treatment of patients with renal failure. | the efficacy and safety of aztreonam for treatment of gram-negative bacterial infections in patients with renal failure were evaluated in an open study. thirty-nine patients (22-82 years old) with renal failure (chronic, 25; acute, 14) were treated for 40 severe gram-negative bacterial infections. all 41 gram-negative pathogens isolated were sensitive to aztreonam; the pathogens included escherichia coli (12), pseudomonas aeruginosa (8), klebsiella species (7), proteus species (7), enterobacter ... | 1991 | 2068477 |
| [determination of the anti-infective action of an immunomodulator. biostim as an example]. | in order to evaluate the antiinfectious action of an immunomodulator, either in vitro or in vivo, in both animal and man, we have to answer three questions: what the targets are? which models best allow the study of the mode of action? which method should be used to evaluate clinical improvement? the targets of ru 41740 (biostim), a purified immunomodulator of biological origin, are the pool of immunocompetent cells with an enhancement of two major mediators: il1 and csf. as there are numerous i ... | 1991 | 2069688 |
| autologous fibrin gel: bactericidal properties in contaminated hepatic injury. | fibrin glue is an effective hemostatic agent in a variety of clinical situations; its utility is limited by potential transmission of viral infection. we studied the bactericidal properties of fibrin gel (fg) in a murine contaminated hepatic injury model and in vitro by agar plate culture method. intra-abdominal abscess formation and adhesion rate were assessed following controlled liver injury in association with abdominal contamination with 10(7) bacteroides fragilis and hepatorrhaphy (h, n = ... | 1991 | 2072441 |
| ceftriaxone-sulbactam combination in rabbit endocarditis caused by a strain of klebsiella pneumoniae producing extended-broad-spectrum tem-3 beta-lactamase. | we studied the activity of the combination of sulbactam and ceftriaxone against a klebsiella pneumoniae strain producing tem-3, a new extended-broad-spectrum beta-lactamase, in an endocarditis model. in vitro, ceftriaxone was strongly inactivated in the presence of tem-3 (mbc, 128 micrograms/ml with an inoculum of 5 x 10(5) cfu/ml). a marked inoculum effect was demonstrated with sulbactam: effective concentrations of inhibitor needed to reduce the mic and mbc of ceftriaxone to similar levels inc ... | 1990 | 2073099 |
| outbreak of ceftazidime resistance caused by extended-spectrum beta-lactamases at a massachusetts chronic-care facility. | during a 4-month period in late 1988, we isolated ceftazidime-resistant strains of klebsiella pneumoniae and other members of the family enterobacteriaceae from 29 patients at a chronic-care facility in massachusetts. ceftazidime resistance resulted from two distinct extended-spectrum beta-lactamases of the tem type which efficiently hydrolyzed the cephalosporin: you-1 with a pi of 5.57 and you-2 with a pi of 5.2. genes encoding these enzymes were present on different but closely related high-mo ... | 1990 | 2073110 |
| development of "oligotyping" for characterization and molecular epidemiology of tem beta-lactamases in members of the family enterobacteriaceae. | based on the dna sequences of blatem-1 and blatem-2, which encode parental penicillinases tem-1 and tem-2, respectively, and blatem-3, blatem-4, blatem-5, blatem-6, and blatem-7, which encode extended-spectrum beta-lactamases, we designed heptadecanucleotides to discriminate point mutations in five loci. determination of the hybridization profiles by colony hybridization with this selection of probes, termed "oligotyping," allowed characterization of the tem variants present in 265 clinical isol ... | 1990 | 2073111 |
| infection control in a third world burn facility. | a study of wound colonization by bacteria in 49 consecutive admissions was conducted over a 2-month period in patients with burns at king edward viii and clairwood hospitals, durban, neither of which possess proper burn units. specimens for culture were collected from patients, staff and the environment. bacteria most frequently isolated were staph. aureus, klebsiella spp., ps. aeruginosa and enterobacter spp. cross-infection occurred due to breakdown of aseptic techniques. staff hands and conta ... | 1990 | 2073344 |
| the in-vitro activity of cefodizime: a review. | for enterobacteriaceae, mic50s and mic90s of cefodizime (mg/l), respectively, were as follows, for naturally non-beta-lactamase-producing species: escherichia coli 0.12 and 0.5, salmonella spp. and shigella spp. 0.25 and 0.5, proteus mirabilis 0.016 and 0.03; for chromosomal penicillinase-producing species. klebsiella spp. 0.25 and 64, and for chromosomal cephalosporinase-producing species. enterobacter cloacae 1 and 64, citrobacter freundii 1 and 128, serratia marcescens 2 and 8: indole-positiv ... | 1990 | 2074248 |
| [analysis of immunoprotection against fatal experimental klebsiella pneumoniae pneumonia]. | comparatively little is known about the immunoprotective mechanisms against k. pneumoniae pneumonia. whether the antibody against k. pneumoniae protects against k. pneumoniae pneumonia is not fully known. this study was designed to investigate some of the murine host factors responsible for immunization with k. pneumoniae dt-s to infection by aerosol inhalation with k. pneumoniae dt-s: in particular, the role of the antibody and the immunoprotective antibody which was the antibody against which ... | 1990 | 2074365 |
| [studies on the sub-mic of beta-lactam antibiotics--bactericidal activity in compromised host's serum]. | bactericidal activity in compromised host's serum i.e. patients with cancer, the elderly, diabetes, was stronger than that in normal serum against proteus mirabilis (p. mirabilis) but was weaker against klebsiella pneumoniae (k. pneumoniae). against escherichia coli (e. coli), bactericidal activity on serum of patients with cancer was weaker in the following order, that in elderly serum, that in diabetic serum. against proteus vulgaris (p. vulgaris), bactericidal activity in elderly serum was si ... | 1990 | 2074367 |
| nosocomial infections in the neonatal intensive care unit department of child health, dr. hasan sadikin general hospital, bandung. | this study was conducted prospectively on neonates admitted to the neonatal intensive care unit, department of child health, dr. hasan sadikin general hospital bandung, during the period of august 1, 1988 until january 31, 1989. the incidence rate was 51.6%. there were 9 males (56%) and 7 females (44%). bacteriemia was the most common type of nosocomial infection and e. coli and klebsiella pneumoniae were the most common etiology. all bacteriae were 100% sensitive to amikacin but 10% resistant t ... | 1990 | 2075020 |
| [clinico-etiological characteristics of intestinal diseases in young children]. | intestinal disorders in infants are determined in 96.9% of cases by the opportunistic flora with the predominance of pr. mirabilis (25.5%), e. coli (19.1%), enterobacter aerogenes (12.6%) and with the less frequent occurrence of klebsiella (4.1%) and pathogenic staphylococci (5.8%). as a result of the unbased use of antibiotics, the majority of children demonstrate dysbacteriosis and the growth of the number of resistant hospital strains. it is suggested that in children with arvi, the drugs con ... | 1990 | 2075044 |
| [determination of lymphocyte receptors to klebsiella in ankylosing spondylitis]. | the authors give the results of determining receptors to klebsiella antigens on the surface of the peripheral blood lymphocytes in ankylosing spondyloarthritis (as). they examined 21 patients with as, 20 patients with rheumatoid arthritis (ra) and 20 healthy individuals. determination of the receptors was done in the reaction of indirect rosette formation. an ultrasonic disintegrate of klebsiella culture isolated from a patient with as was used as an antigen. favourable results were obtained in ... | 1990 | 2075352 |
| [the main characteristics of outbreaks of hospital infections in the ussr in 1986-1988]. | the analysis of 69 outbreaks of hospital infections in different regions of the ussr in 1986-1989 (the number of patients affected by these infections was 1,517) showed that most of these outbreaks (41) occurred at hospital wards for newborn infants. acute enteric infections prevailed in the total structure of the outbreaks (73.7%). purulent septic infections among newborns constituted a quarter of all outbreaks. opportunistic microbes, and mainly klebsiella, were the dominating element in the e ... | 1990 | 2075767 |
| transcriptional analysis of the glnb-glna region of rhizobium leguminosarum biovar viciae. | we report that the glnb and glna genes of rhizobium leguminosarum biovar viciae are preceded by promoters located upstream of each gene. we find the presence of a glnb-glna and a glna mrna whose intracellular concentration changes two- to three-fold when r. leguminosarum is grown on different nitrogen sources. primer extension analysis shows unique transcriptional initiation sites upstream of glnb and glna. the glnb promoter is rpon(ntra)-dependent, while the glna promoter does not contain a typ ... | 1990 | 2077357 |
| chromotropic character of bacterial acidic polysaccharides: part iii--interaction of cationic dye pinacyanol chloride with klebsiella k15 capsular polysaccharide. | interaction of cationic dye pinacyanol chloride with the acidic capsular polysaccharide isolated from klebsiella serotype k15 has been investigated by spectral measurements. klebsiella k15 polysaccharide consists of hexasaccharide repeating units containing one residue each of glucuronic acid and glucose, and four residues of galactose. glucuronic acid acts as the potential anionic site and the biopolymer interacts with the dye cations. it induces metachromasy in the dye and a blue shift of abou ... | 1990 | 2079334 |
| [effectiveness and tolerance of cefixime in the treatment of acute pyelonephritis]. | in a prospective, open clinical study, 50 urological patients with acute pyelonephritis were treated with the oral cephalosporin cefixime. the medication (2 x 200 mg/day) was given for seven to ten days. clinical, bacteriological as well as hematological examinations were carried out prior to, during and immediately after therapy. a late check-up was performed five to nine days after the end of therapy. 46 of the 50 cases were evaluable for efficacy, and all 50 patients were included in safety e ... | 1990 | 2079374 |
| prevalence of a transferable shv-5 type beta-lactamase in clinical isolates of klebsiella pneumoniae and escherichia coli in greece. | analysis with a double-disc synergy test (ddst) of clinical isolates of klebsiella pneumoniae and escherichia coli during the period october 1988-september 1989 revealed that 24% of the former and 4% of the latter, mainly isolated from urine, possessed an extended-spectrum beta-lactamase. during this period no ddst was positive for isolates of other enterobacterial species. transfer of ceftazidime resistance was demonstrated from six k. pneumoniae and two e. coli isolates resistant to third gene ... | 1990 | 2079446 |
| prevalence and antimicrobial susceptibility of enterobacteriaceae, pseudomonadaceae and acinetobacter in human periodontitis. | this study examined the occurrence of non-oral gram-negative facultatively anaerobic rods in advanced adult periodontitis. speciation and in vitro antimicrobial susceptibility testing was performed using the microscan micromethod system. a total of 42 taxa of enterobacteriaceae, pseudomonadaceae and acinetobacter were isolated from 427 of 3,050 (14.0%) patients. in 159 (5.2%) patients, these organisms comprised more than 5% of the cultivable subgingival microflora. enterobacter cloacae, klebsiel ... | 1990 | 2080069 |
| protection against klebsiella pneumoniae induced lobar pneumonia in rats with lipopolysaccharide and related antigens. | the immunoprotective role of lipopolysaccharide and related antigens from klebsiella pneumoniae was studied in a lobar pneumonia model developed in rats. various antigens were obtained by different chemical treatments of the lipopolysaccharide. all these antigens (purified lipopolysaccharide, reduced lipopolysaccharide, lipopolysaccharide--bovine serum albumin complex, and lipid a--bovine serum albumin complex were tested for pyrogenicity and the shwartzman reaction. the lipopolysaccharide and t ... | 1990 | 2081334 |
| trimethoprim, sulphamethoxazole, bacterial adhesion and polymorphonuclear leucocyte function. | culturing escherichia coli, proteus mirabilis and klebsiella pneumoniae in the presence of subinhibitory concentrations (1/4 mic) of trimethoprim, sulphamethoxazole or their combination, resulted in reduced adherence of all the above strains. the number of phagocytosed bacteria pre-exposed to subinhibitory concentrations of the above antibiotics was not significantly changed, but a significant increase of bactericidal activity of the polymorphonuclear leucocytes was observed. furthermore, filtra ... | 1990 | 2081720 |
| decontamination of the bowel by intravenous administration of pefloxacin. | intravenous administration of pefloxacin 400 mg twice daily rapidly decontaminated the bowel from gram-negative bacilli in ten healthy volunteers. the faecal concentrations of enterococci and yeasts did not change significantly. further, pefloxacin did not facilitate colonization of the bowel by a highly resistant challenge strain (klebsiella pneumoniae, mic = 56 mg/l). the diffusible faecal concentration of pefloxacin was between 110 and 260 mg/l in all samples from day 3 of treatment onwards. ... | 1990 | 2081723 |
| transcriptional regulatory cascade of nitrogen-fixation genes in anoxygenic photosynthetic bacteria: oxygen- and nitrogen-responsive factors. | many photosynthetic bacteria from aquatic and terrestrial habitats reduce atmospheric dinitrogen to ammonia. the synthesis of proteins required for nitrogen fixation in these microorganisms is repressed by fixed nitrogen or oxygen. studies on the purple non-sulphur phototroph rhodobacter capsulatus have helped to clarify this transcriptional control and to define the factors involved in this regulation. the molecular mechanisms by which the nitrogen and oxygen status of the cell are relayed into ... | 1990 | 2082142 |
| degradation of the metal-cyano complex tetracyanonickelate(ii) by cyanide-utilizing bacterial isolates. | ten bacterial isolates capable of growth on tetracyanonickelate(ii) [k2[ni(cn)4]] (tcn) as the sole nitrogen source were isolated from soil, freshwater, and sewage sludge enrichments. seven of the 10 were identified as pseudomonads, while the remaining 3 were classified as klebsiella species. a detailed investigation of one isolate, pseudomonas putida bcn3, revealed a rapid growth rate on tcn (generation time, 2 h), with substrate removal and growth occurring in parallel. in addition to tcn, all ... | 1990 | 2082819 |
| remnants of an ancient pathway to l-phenylalanine and l-tyrosine in enteric bacteria: evolutionary implications and biotechnological impact. | the pathway construction for biosynthesis of aromatic amino acids in escherichia coli is atypical of the phylogenetic subdivision of gram-negative bacteria to which it belongs (r. a. jensen, mol. biol. evol. 2:92-108, 1985). related organisms possess second pathways to phenylalanine and tyrosine which depend upon the expression of a monofunctional chorismate mutase (cm-f) and cyclohexadienyl dehydratase (cdt). some enteric bacteria, unlike e. coli, possess either cm-f or cdt. these essentially c ... | 1990 | 2082822 |
| purification and characterization of a novel nucleoside phosphorylase from a klebsiella sp. and its use in the enzymatic production of adenine arabinoside. | an adenosine-assimilating bacterium, klebsiella sp. strain lf1202, inducibly formed a novel nucleoside phosphorylase which acted on both purine and pyrimidine nucleosides when the cells were cultured in medium containing adenosine as a sole source of carbon and nitrogen. the enzyme was purified (approximately 83-fold, with a 17% activity yield) to the homogeneous state by polyacrylamide gel electrophoresis. the molecular weight of the purified enzyme was calculated to be 125,000 by gel filtratio ... | 1990 | 2082829 |
| in-vitro susceptibility patterns of some major respiratory tract pathogens in nigeria to eleven selected antibiotics. | a study of the antibiotic sensitivity patterns of nine pathogens isolated from bronchopulmonary infections was made using the disc diffusion method. erythromycin, carbenicillin and chloramphenicol were in that sequence the drugs most effective against the gram-positive cocci, followed by ampicillin to which however, 56% of the strains of staphylococcus aureus tested showed resistance. more than 95% of the strains of haemophilus influenza were very susceptible to carbenicillin and chloramphenicol ... | 1990 | 2083203 |
| in vitro transcription/translation of the alginate lyase gene from klebsiella pneumoniae and detection of a precursor form of the enzyme. | | 1990 | 2083741 |
| heterologous expression and cellular distribution in escherichia coli of the gene from klebsiella pneumoniae which encodes alginate lyase. | | 1990 | 2083742 |
| clinical and bacteriological profile of hospitalized community acquired pneumonias--a preliminary study. | the spectrum of clinical and bacteriological features of 46 cases of pneumonias presenting to u.c.m.s. and guru tegh bahadur hospital complex in east delhi over a period of three months are presented and their etiopathogenesis discussed. the high incidence of pneumonias in admitted patients belonging to the population adjacent to the hospital is highlighted. etiological diagnosis based on sputum culture and sensitivity tests was possible only in 37% cases, and there is need for the use of newer ... | 1990 | 2083965 |
| [microbiocenosis and the status of the intestinal mucosa in alkylosing spondyloarthritis]. | intestinal microbiocenosis was examined in 53 patients with ankylosing spondyloarthritis (as). of these, 23 had the central and 30 presented with the peripheral form. 34 patients underwent endoscopic examinations (rectoromanoscopy, ileocolonoscopy). as a result, in 74% of cases with the central form of as and in 80% of cases with the peripheral form of as, alterations in the intestinal microflora were revealed, which can be regarded as intestinal dysbacteriosis (id), with dysbacteriosis being mo ... | 1990 | 2084929 |
| [value of microbiologic studies for diagnosis of post-enteritis reactive arthritis]. | reactive arthritis may develop within a period of some days until upto 3 weeks after infections with yersinia enterocolitica, yersinia pseudotuberculosis, campylobacter jejuni/coli, shigella and salmonella. intestinal infections with klebsiella pneumoniae, clostridium perfringens, clostridium difficile, cryptosporidium, strongyloides stercoralis, taenia saginata and schistosoma mansoni are, in some cases, considered to be responsible for reactive arthritis. detection of pathogenic bacteria in fe ... | 1990 | 2085058 |