| [a study of the comparative activity of five antibiotics alone and in certain combinations against escherichia coli, aerobacter aerogenes pseudomonas aeruginosa and proteus sp]. | | 1952 | 24542051 |
| heat resistance of histamine-producing bacteria in irradiated tuna loins. | consumption of foods high in biogenic amines leads to an illness known as histamine, or scombrotoxin, poisoning. the illness is commonly associated with consumption of fish with high levels of histamine ( $ 500 ppm). the objective of this study was to determine and compare the heat resistance of five histamine-producing bacteria in irradiated albacore tuna loins. heat-resistance parameters (d- and z-values) were determined for morganella morganii, raoultella planticola, hafnia alvei, and enterob ... | 2013 | 23992506 |
| non-thiolate ligation of nickel by nucleotide-free ureg of klebsiella aerogenes. | nickel-dependent ureases are activated by a multiprotein complex that includes the gtpase ureg. prior studies showed that nucleotide-free ureg from klebsiella aerogenes is monomeric and binds one nickel or zinc ion with near-equivalent affinity using an undefined binding site, whereas nucleotide-free ureg from helicobacter pylori selectively binds one zinc ion per dimer via a universally conserved cys-pro-his motif in each protomer. iodoacetamide-treated k. aerogenes ureg was nearly unaffected i ... | 2016 | 28004186 |
| mutational and computational evidence that a nickel-transfer tunnel in ured is used for activation of klebsiella aerogenes urease. | nickel-containing urease from klebsiella aerogenes requires four accessory proteins for proper active site metalation. the metallochaperone uree delivers nickel to ureg, a gtpase that forms a ured/uref/ureg complex, which binds to urease apoprotein via ured. prior in silico analysis of the homologous, structurally characterized ureh/uref/ureg complex from helicobacter pylori identified a water tunnel originating at a likely nickel-binding motif in ureg, passing through uref, and exiting ureh, su ... | 2015 | 26401965 |
| reduction of urease activity by interaction with the flap covering the active site. | with the increasing appreciation for the human microbiome coupled with the global rise of antibiotic resistant organisms, it is imperative that new methods be developed to specifically target pathogens. to that end, a novel computational approach was devised to identify compounds that reduce the activity of urease, a medically important enzyme of helicobacter pylori, proteus mirabilis, and many other microorganisms. urease contains a flexible loop that covers its active site; glide was used to i ... | 2015 | 25594724 |
| molecular dynamics study of helicobacter pylori urease. | helicobacter pylori have been implicated in an array of gastrointestinal disorders including, but not limited to, gastric and duodenal ulcers and adenocarcinoma. this bacterium utilizes an enzyme, urease, to produce copious amounts of ammonia through urea hydrolysis in order to survive the harsh acidic conditions of the stomach. molecular dynamics (md) studies on the h. pylori urease enzyme have been employed in order to study structural features of this enzyme that may shed light on the hydroly ... | 2014 | 24839409 |
| a new hexahydrobenzopyran derivative from the gorgonian-derived fungus eutypella sp. | a new hexahydrobenzopyran derivative cytosporin l (1), and another eight known compounds were isolated from the gorgonian-derived fungus eutypella sp. collected from the south china sea. the structure of 1 was elucidated by comprehensive spectroscopic data. the absolute configuration of 1 was determined by a chemical reaction and the modified mosher's method. the isolated secondary metabolites were evaluated for their antibacterial activities. compound 1 showed antibacterial activity towards mic ... | 2017 | 28278631 |
| presence, distribution and molecular epidemiology of multi-drug-resistant gram-negative bacilli from medical personnel of intensive care units in tianjin, china, 2007-2015. | multi-drug-resistant gram-negative bacteria (mdrgnb) have become an important cause of nosocomial infection in intensive care units (icus). | 2017 | 28268024 |
| quantifying bacterial cross-contamination rates between fresh-cut produce and hands. | this study quantifies the cross-contamination rates between fresh-cut produce and hands using a nalidixic acid-resistant nonpathogenic enterobacter aerogenes and cocktails of rifampin-resistant salmonella or escherichia coli o157:h7 strains. volunteers performed the e. aerogenes experiments (n = 20), and one of the authors performed the salmonella and e. coli o157:h7 experiments multiple times (n =15 and n =10, respectively). each participant handled 25 g of fresh-cut carrots, celery, or cantalo ... | 2017 | 28221987 |
| a biological method for in-situ synthesis of hydroxyapatite-coated magnetite nanoparticles using enterobacter aerogenes: characterization and acute toxicity assessments. | hydroxyapatite (ha)-coated magnetite nanoparticles (mnps) are being widely investigated for various applications in medical engineering and wastewater treatment. in this work, the mnps were thoroughly coated by bacterial synthesized ha nanoparticles during biomineralization process using enterobacter aerogenes. the resulting bacterial-induced precipitate was then calcined at 600°c and investigated with respect to structural characteristics, particle size and magnetic strength by xrd, ft-ir, sem, ... | 2017 | 28183602 |
| motuporamine derivatives as antimicrobial agents and antibiotic enhancers against resistant gram-negative bacteria. | dihydromotuporamine c and its derivatives were evaluated for their in vitro antimicrobial activities and antibiotic enhancement properties against gram-negative bacteria and clinical isolates. the mechanism of action of one of these derivatives, motu-n44, was investigated against enterobacter aerogenes by using fluorescent dyes to evaluate outer-membrane depolarization and permeabilization. its efficiency correlated with inhibition of dye transport, thus suggesting that these molecules inhibit d ... | 2017 | 28098416 |
| optimization of organosolv pretreatment of rice straw for enhanced biohydrogen production using enterobacter aerogenes. | ethanol organosolv pretreated rice straw was used to produce biohydrogen using enterobacter aerogenes. the effect of temperature (120-180°c), residence time (30-90min), and ethanol concentration (45-75%v/v) on the hydrogen yield, residual biomass, and lignin recovery was investigated using rsm. in contrast to the residual solid and lignin recovery, no considerable trend could be observed for the changes in the hydrogen yield at different treatment severities. the maximum hydrogen yield of 19.73m ... | 2017 | 28042989 |
| etiology of early onset septicemia among neonates at the university college hospital, ibadan, nigeria. | neonatal septicemia remains a major cause of newborn deaths in developing countries. its burden is further compounded by the emergence of multidrug-resistant pathogens, which is related to a lack of antibiotic protocols resulting in unrestricted use of antibiotics. the absence of reliable antibiotic sensitivity testing makes the formulation of antibiotic guidelines and judicious use of antibiotics difficult. this study sought to identify the current bacterial agents associated with early onset s ... | 2016 | 28036314 |
| enhanced hydrogen production of enterobacter aerogenes mutated by nuclear irradiation. | nuclear irradiation was used for the first time to generate efficient mutants of hydrogen-producing bacteria enterobacter aerogenes, which were screened with larger colour circles of more fermentative acid by-products. e. aerogenes cells were mutated by nuclear irradiation of (60)co γ-rays. the screened e. aerogenes zju1 mutant with larger colour circles enhanced the hydrogenase activity from 89.8 of the wild strain to 157.4mlh2/(gdwh). the hereditary stability of the e. aerogenes zju1 mutant wa ... | 2017 | 28013136 |
| enterobacter aerogenes hormaeche and edwards 1960 (approved lists 1980) and klebsiella mobilis bascomb et al. 1971 (approved lists 1980) share the same nomenclatural type (atcc 13048) on the approved lists and are homotypic synonyms, with consequences for the name klebsiella mobilis bascomb et al. 1971 (approved lists 1980). | enterobacter aerogenes hormaeche and edwards 1960 (approved lists 1980) and klebsiella mobilis bascomb et al. 1971 (approved lists 1980) were placed on the approved lists of bacterial names and were based on the same nomenclatural type, atcc 13048. consequently they are to be treated as homotypic synonyms. however, the names of homotypic synonyms at the rank of species normally are based on the same epithet. examination of the rules of the international code of nomenclature of bacteria in force ... | 2017 | 27902205 |
| genome sequencing of four multidrug-resistant enterobacter aerogenes isolates from hospitalized patients in brazil. | | 2016 | 27833588 |
| in vivo acquisition of carbapenemase gene blakpc-2 in multiple species of enterobacteriaceae through horizontal transfer of insertion sequence or plasmid. | objectives: current worldwide spread of carbapenem resistance in enterobacteriaceae constitutes a critical public health threat. this study aims to investigate how carbapenem resistance is acquired in enterobacteriaceae in patients during antimicrobial therapy. methods: clinical strains from the same anatomical site of the same patients that converted from carbapenem-susceptible to resistant during antimicrobial therapy and showed identical or similar pfge patterns were identified. the similarly ... | 2016 | 27818649 |
| neonatal sepsis in haiti. | infections (including sepsis, meningitis, pneumonia and tetanus) stand as a major contributor to neonatal mortality in haiti (22%). infants acquire bacteria that cause neonatal sepsis directly from the mother's blood, skin or vaginal tract either before or during delivery. nosocomial and environmental pathogens introduce further risk after delivery. the absence of cohesive medical systems and methods for collecting information limits the available data in countries such as haiti. this study seek ... | 2017 | 27765888 |
| longer contact times increase cross-contamination of enterobacter aerogenes from surfaces to food. | bacterial cross-contamination from surfaces to food can contribute to foodborne disease. the cross-contamination rate of enterobacter aerogenes on household surfaces was evaluated by using scenarios that differed by surface type, food type, contact time (<1, 5, 30, and 300 s), and inoculum matrix (tryptic soy broth or peptone buffer). the surfaces used were stainless steel, tile, wood, and carpet. the food types were watermelon, bread, bread with butter, and gummy candy. surfaces (25 cm(2)) were ... | 2016 | 27590818 |
| detection of new delhi metallo-beta-lactamase (encoded by blandm-1 ) in enterobacter aerogenes in china. | the increase in blandm-1 in enterobacteriaceae has become a major concern worldwide. in previous study, we investigated clonal dissemination and mechanisms of resistance to carbapenem in china. | 2017 | 27565452 |
| bioprospecting of lipolytic microorganisms obtained from industrial effluents. | the lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. the aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in pelotas, rs/brazil. bacteria were identified using gram stain and biochemical tests (vitek 2(r)). fungi were identified according to macro and micromorphology characteristics. the extracellular lipase production was evaluated using the rhoda ... | 2016 | 27556331 |
| the melatonin-sensitive circadian clock of the enteric bacterium enterobacter aerogenes. | circadian clocks are fundamental properties of all eukaryotic organisms and at least some prokaryotic organisms. recent studies in our laboratory have shown that the gastrointestinal system contains a circadian clock that controls many, if not all, aspects of gastrointestinal function. we now report that at least one species of intestinal bacteria, enterobacter aerogenes, responds to the pineal and gastrointestinal hormone melatonin by an increase in swarming activity. this swarming behavior is ... | 2016 | 27387841 |
| physiological and molecular characteristics of carbapenem resistance in klebsiella pneumoniae and enterobacter aerogenes. | bacterial resistance is a growing concern in the nosocomial environment in which klebsiella pneumoniae and enterobacter aerogenes play an important role due to their opportunism and carbapenemase-production. this work aimed to evaluate physiological and molecular characteristics of carbapenem-resistant k. pneumoniae and e. aerogenes isolated in a brazilian tertiary hospital. | 2016 | 27367007 |
| enhancing hydrogen production of enterobacter aerogenes by heterologous expression of hydrogenase genes originated from synechocystis sp. | the hydrogenase genes (hoxefuyh) of synechocystis sp. pcc 6803 were cloned and heterologously expressed in enterobacter aerogenes atcc13408 for the first time in this study, and the hydrogen yield was significantly enhanced using the recombinant strain. a recombinant plasmid containing the gene in-frame with glutathione-s-transferase (gst) gene was transformed into e. aerogenes atcc13408 to produce a gst-fusion protein. sds-page and western blot analysis confirm the successful expression of the ... | 2016 | 27343449 |
| optimization of l-asparaginase production from novel enterobacter sp., by submerged fermentation using response surface methodology. | the culture conditions and nutritional rations influencing the production of extra cellular antileukemic enzyme by novel enterobacter aerogenes kctc2190/mtcc111 were optimized in shake-flask culture. process variables like ph, temperature, incubation time, carbon and nitrogen sources, inducer concentration, and inoculum size were taken into account. in the present study, finest enzyme activity achieved by traditional one variable at a time method was 7.6 iu/ml which was a 2.6-fold increase compa ... | 2017 | 27340934 |
| isolation of kpc 3-producing enterobacter aerogenes in a patient colonized by mdr klebsiella pneumoniae. | we describe the interspecies transmission of the plasmid-mediated blakpc-3 gene, which confers carbapenem resistance, between clinically relevant gram-negative bacteria in a single patient. a kpc-3 producing enterobacter aerogenes was isolated from a hospitalized patient previously colonized and then infected by a klebsiella pneumoniae st101 carrying the blakpc-3 gene. the strains showed identical plasmids. since intense horizontal exchanges among bacteria can occur in the gut, clinicians should ... | 2016 | 27284988 |
| efflux pump blockers in gram-negative bacteria: the new generation of hydantoin based-modulators to improve antibiotic activity. | multidrug resistant (mdr) bacteria are an increasing health problem with the shortage of new active antibiotic agents. among effective mechanisms that contribute to the spread of mdr gram-negative bacteria are drug efflux pumps that expel clinically important antibiotic classes out of the cell. drug pumps are attractive targets to restore the susceptibility toward the expelled antibiotics by impairing their efflux activity. arylhydantoin derivatives were investigated for their potentiation of ac ... | 2016 | 27199950 |
| occurrence and analysis of irp2 virulence gene in isolates of klebsiella pneumoniae and enterobacter spp. from microbiota and hospital and community-acquired infections. | eighty-five isolates of klebsiella pneumoniae and enterobacter spp., originating from hospital- and community-acquired infections and from oropharyngeal and faecal microbiota from patients in recife-pe, brazil, were analyzed regarding the presence of irp2 gene. this is a yersinia typical gene involved in the synthesis of siderophore yersiniabactin. dna sequencing confirmed the identity of irp2 gene in five k. pneumoniae, five enterobacter aerogenes and one enterobacter amnigenus isolates. to our ... | 2016 | 27133266 |
| multidrug-resistant combined infections in a liver transplanted patient: case report. | we report a case of successfully treated multiple liver abscesses in a liver-transplanted patient, sustained by combined multidrug-resistant infections. two months after a liver transplant, a computed tomography scan revealed the presence of multiple abscesses in the liver graft. blood cultures and abscessual liver fluid were both positive for acquired colistin- and carbapenem- resistant klebsiella pneumoniae and an extended-spectrum of beta-lactamases-producing enterobacter aerogenes. the treat ... | 2016 | 27063638 |
| isolation of enterobacter aerogenes carrying blatem-1 and blakpc-3 genes recovered from a hospital intensive care unit. | enterobacter aerogenes has recently emerged as an important hospital pathogen. in this study, we showed the emergence of e. aerogenes isolates carrying the blakpc gene in patients colonized by carbapenem-resistant klebsiella pneumoniae strains. two multiresistant e. aerogenes isolates were recovered from bronchial aspirates of two patients hospitalized in the intensive care unit at the "santa maria della scaletta" hospital, imola. the antimicrobial susceptibility test showed the high resistance ... | 2016 | 27004836 |
| osteoconductive composite graft based on bacterial synthesized hydroxyapatite nanoparticles doped with different ions: from synthesis to in vivo studies. | to repair damaged bone tissues, osteoconductive bone graft substitutes are required for enhancement of the regenerative potential of osteoblast cells. nanostructured hydroxyapatite is a bioactive ceramic used for bone tissue engineering purposes. in this study, carbonate hydroxyapatite (cha) and zinc-magnesium substituted hydroxyapatite (zn-mg-ha) nanoparticles were synthesized via biomineralization method using enterobacter aerogenes. the structural phase composition and the morphology of the s ... | 2016 | 26956413 |
| trapping and viability of swimming bacteria in an optoelectric trap. | non-contact manipulation methods capable of trapping and transporting swimming bacteria can significantly aid in chemotaxis studies. however, high swimming speed makes the trapping of these organisms an inherently challenging task. we demonstrate that an optoelectric technique, rapid electrokinetic patterning (rep), can effectively trap and manipulate enterobacter aerogenes bacteria swimming at velocities greater than 20 μm s(-1). rep uses electro-orientation, laser-induced ac electrothermal flo ... | 2016 | 26891971 |
| enhanced dark hydrogen fermentation by addition of ferric oxide nanoparticles using enterobacter aerogenes. | ferric oxide nanoparticles (fonps) were used to facilitate dark hydrogen fermentation using enterobacter aerogenes. the hydrogen yield of glucose increased from 164.5±2.29 to 192.4±1.14ml/g when fonps concentration increased from 0 to 200mg/l. sem images of e. aerogenes demonstrated the existence of bacterial nanowire among cells, suggesting fonps served as electron conduits to enhance electron transfer. tem showed cellular internalization of fonps, indicating hydrogenase synthesis and activity ... | 2016 | 26890796 |
| molecular docking of glycine max and medicago truncatula ureases with urea; bioinformatics approaches. | urease (ec 3.5.1.5) is a nickel-dependent metalloenzyme catalyzing the hydrolysis of urea into ammonia and carbon dioxide. it is present in many bacteria, fungi, yeasts and plants. most species, with few exceptions, use nickel metalloenzyme urease to hydrolyze urea, which is one of the commonly used nitrogen fertilizer in plant growth thus its enzymatic hydrolysis possesses vital importance in agricultural practices. considering the essentiality and importance of urea and urease activity in most ... | 2016 | 26852122 |
| adequate hand washing and glove use are necessary to reduce cross-contamination from hands with high bacterial loads. | hand washing and glove use are the main methods for reducing bacterial cross-contamination from hands to ready-to-eat food in a food service setting. however, bacterial transfer from hands to gloves is poorly understood, as is the effect of different durations of soap rubbing on bacterial reduction. to assess bacterial transfer from hands to gloves and to compare bacterial transfer rates to food after different soap washing times and glove use, participants' hands were artificially contaminated ... | 2016 | 26818993 |
| mutational analysis of the hyc-operon determining the relationship between hydrogenase-3 and nadh pathway in enterobacter aerogenes. | in this study, the hydrogenase-3 gene cluster (hycdefgh) was isolated and identified from enterobacter aerogenes cctcc ab91102. all gene products were highly homologous to the reported bacterial hydrogenase-3 (hyd-3) proteins. the genes hyce, hycf, hycg encoding the subunits of hydrogenase-3 were targeted for genetic knockout to inhibit the fhl hydrogen production pathway via the red recombination system, generating three mutant strains ab91102-e (δhyce), ab91102-f (δhycf) and ab91102-g (δhycg). ... | 2016 | 26672442 |
| ca(ii) binding regulates and dominates the reactivity of a transition-metal-ion-dependent diesterase from mycobacterium tuberculosis. | the diesterase rv0805 from mycobacterium tuberculosis is a dinuclear metallohydrolase that plays an important role in signal transduction by controlling the intracellular levels of cyclic nucleotides. as rv0805 is essential for mycobacterial growth it is a promising new target for the development of chemotherapeutics to treat tuberculosis. the in vivo metal-ion composition of rv0805 is subject to debate. here, we demonstrate that the active site accommodates two divalent transition metal ions wi ... | 2016 | 26662456 |
| phenolic compounds: strong inhibitors derived from lignocellulosic hydrolysate for 2,3-butanediol production by enterobacter aerogenes. | lignocellulosic biomass are attractive feedstocks for 2,3-butanediol production due to their abundant supply and low price. during the hydrolysis of lignocellulosic biomass, various byproducts are formed and their effects on 2,3-butanediol production were not sufficiently studied compared to ethanol production. therefore, the effects of compounds derived from lignocellulosic biomass (weak acids, furan derivatives and phenolics) on the cell growth, the 2,3-butanediol production and the enzymes ac ... | 2015 | 26479290 |
| insights into the global regulation of anaerobic metabolism for improved biohydrogen production. | to improve the biohydrogen yield in bacterial dark fermentation, a new approach of global anaerobic regulation was introduced. two cellular global regulators fnr and narp were overexpressed in two model organisms: facultatively anaerobic enterobacter aerogenes (ea) and strictly anaerobic clostridium paraputrificum (cp). the overexpression of fnr and narp greatly altered anaerobic metabolism and increased the hydrogen yield by 40%. metabolic analysis showed that the global regulation caused more ... | 2016 | 26476162 |
| purification and antibiofilm activity of ahl-lactonase from endophytic enterobacter aerogenes vt66. | the opportunistic pathogen pseudomonas aeruginosa uses biofilm lifestyle to resist antibiotic treatment. in our study, endophytic bacterium enterobacter aerogenes vt66 quenched the n-acyl homoserine lactone (ahl) molecules produced by p. aeruginosa pao1. the quorum quenching activity was attributed to the presence of ahl-lactonase. the ahl-lactonase was purified using column chromatography and purified ahl-lactonase was applied for the control of biofilm formation in p. aeruginosa pao1. the resu ... | 2015 | 26432367 |
| prevalence and characterization of high histamine-producing bacteria in gulf of mexico fish species. | recent developments in detection and enumeration of histamine-producing bacteria (hpb) have created powerful molecular-based tools to better understand the presence of spoilage bacteria and conditions, resulting in increased risk of scombrotoxin fish poisoning. we examined 235 scombrotoxin-forming fish from the gulf of mexico for the presence of high hpb. photobacterium damselae subsp. damselae was the most prevalent hpb (49%), followed by morganella morganii (14%), enterobacter aerogenes (4%), ... | 2015 | 26197285 |
| disruption of lactate dehydrogenase and alcohol dehydrogenase for increased hydrogen production and its effect on metabolic flux in enterobacter aerogenes. | hydrogen production by enterobacter aerogenes from glucose was enhanced by deleting the targeted ldha and adh genes responsible for two nadh-consuming pathways which consume most nadh generated from glycolysis. compared with the wild-type, the hydrogen yield of iam1183-δldha increased 1.5 fold. metabolic flux analysis showed both iam1183-δldha and iam1183-δadh exhibited significant changes in flux, including enhanced flux towards the hydrogen generation. the lactate production of iam1183-δldha s ... | 2015 | 26188552 |
| impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in enterobacter aerogenes. | succinate is an important c4 building block chemical, and its production via fermentative processes in bacteria has many practical applications in the biotechnology field. one of the major goals of optimizing the bacterium-based succinate production process is to lower the culture ph from the current neutral conditions, as this would reduce total production costs. in our previous studies, we selected enterobacter aerogenes, a rapid glucose assimilator at ph 5.0, in order to construct a metabolic ... | 2015 | 26063229 |
| [emphysematous cystitis: report of one case]. | emphysematous cystitis is found in diabetic patients and in individuals with urinary stasis and immunosuppression. we report a 58-year-old male with hypertension, type 2 diabetes on insulin treatment and central nervous system vasculitis on immunosuppressive therapy. he was admitted with weight loss and gait instability. a pet-ct showed a circumscribed image of air in the bladder contour without involving the upper urinary tract, suggesting emphysematous cystitis. re-interrogated, the patient re ... | 2015 | 26005827 |
| biohydrogen and bioethanol production from biodiesel-based glycerol by enterobacter aerogenes in a continuous stir tank reactor. | crude glycerol from the biodiesel manufacturing process is being produced in increasing quantities due to the expanding number of biodiesel plants. it has been previously shown that, in batch mode, semi-anaerobic fermentation of crude glycerol by enterobacter aerogenes can produce biohydrogen and bioethanol simultaneously. the present study demonstrated the possible scaling-up of this process from small batches performed in small bottles to a 3.6-l continuous stir tank reactor (cstr). fresh feed ... | 2015 | 25970750 |
| ndm-1 encoded by a pndm-bj01-like plasmid p3sp-ndm in clinical enterobacter aerogenes. | a carbapenem-nonsusceptible enterobacter aerogenes strain named 3-sp was isolated from a human case of pneumonia in a chinese teaching hospital. ndm-1 carbapenemase is produced by a pndm-bj01-like conjugative plasmid designated p3sp-ndm to account for carbapenem resistance of 3-sp. p3sp-ndm was fully sequenced and compared with all publically available pndm-bj01-like plasmids. the genetic differences between p3sp-ndm and pndm-bj01 include only 18 single nucleotide polymorphisms, a 1 bp deletion ... | 2015 | 25926823 |
| draft genome sequence of enterobacter aerogenes, a dde-degrading and plant growth-promoting strain isolated from cucurbita pepo. | we report here the draft genome of enterobacter aerogenes, a gram-negative bacterium of the enterobacteriaceae isolated from cucurbita pepo root tissue. this bacterium shows 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (dde)-degrading potential and plant growth-promoting capacity. an analysis of its 4.5-mb draft genome will enhance the understanding of dde degradation pathways and phytoremediation applications for dde-contaminated soils. | 2015 | 25883299 |
| comparison of validated polymerase chain reaction and culture isolation for the routine detection of acanthamoeba from ocular samples. | acanthamoeba keratitis should be definitively diagnosed for appropriate therapy. our institution has validated polymerase chain reaction (pcr) as a routine diagnostic test to detect acanthamoeba dna from ocular samples. we compared pcr with culture isolation for detecting acanthamoeba from ocular samples. | 2015 | 25839345 |
| quantifying the effect of hand wash duration, soap use, ground beef debris, and drying methods on the removal of enterobacter aerogenes on hands. | hand washing is recognized as a crucial step in preventing foodborne disease transmission by mitigating crosscontamination among hands, surfaces, and foods. this research was undertaken to establish the importance of several keys factors (soap, soil, time, and drying method) in reducing microorganisms during hand washing. a nonpathogenic nalidixic acid-resistant enterobacter aerogenes surrogate for salmonella was used to assess the efficacy of using soap or no soap for 5 or 20 s on hands with or ... | 2015 | 25836392 |
| kpc-producing enterobacter aerogenes infection. | enterobacter is a common nosocomial microorganism and its carbapenem's resistance has increased. the management of these cases is unclear. | 2015 | 25722130 |
| engineering tobacco to remove mercury from polluted soil. | tobacco is an ideal plant for modification to remove mercury from soil. although several transgenic tobacco strains have been developed, they either release elemental mercury directly into the air or are only capable of accumulating small quantities of mercury. in this study, we constructed two transgenic tobacco lines: ntk-7 (a tobacco plant transformed with mert-merp-merb1-merb2-ppk) and ntp-36 (tobacco transformed with mert-merp-merb1-merb2-pcs1). the genes mert, merp, merb1, and merb2 were o ... | 2015 | 25690353 |
| comparison of microbiological and probiotic characteristics of lactobacilli isolates from dairy food products and animal rumen contents. | lactobacilli are employed in probiotic food preparations and as feed additives in poultry and livestock, due to health benefits associated with their consumption. the objective of this study was to evaluate and compare the probiotic potential of ten lactobacilli strains isolated from commercial dairy food products and animal rumen contents in new zealand. genetic identification of the isolates revealed that all belonged to the genus lactobacillus, specifically the species l. reuteri, l. rhamnosu ... | 2015 | 27682086 |
| polygalacturonase production by calcium alginate immobilized enterobacter aerogenes nbo2 cells. | bacterial cells of enterobacter aerogenes nbo2 were entrapped in calcium alginate beads in order to enhance polygalacturonase production compared to free cells. the optimized condition of 5 % (w/v) sodium alginate concentration, agitation speed of 250 rpm, and 15 beads of calcium alginate with inoculum size of 4 % (v/v; 5.4 × 10(7) cells/ml) produced 23.48 u/ml of polygalacturonase compared to free cells of 18.54 u/ml. there was about 26.6 % increment in polygalaturonase production. however, in ... | 2015 | 25547814 |
| screening of wild type streptomyces isolates able to overproduce clavulanic acid. | the selection of new microorganisms able to produce antimicrobial compounds is hoped for to reduce their production costs and the side effects caused by synthetic drugs. clavulanic acid is a β-lactam antibiotic produced by submerged culture, which is widely used in medicine as a powerful inhibitor of β-lactamases, enzymes produced by bacteria resistant to antibiotics such penicillin and cephalosporin. the purpose of this work was to select the best clavulanic acid producer among strains of strep ... | 2014 | 25477926 |
| antioxidant and antibacterial potential of pomegranate peel extracts. | pomegranate peels of ganesh variety were subjected to extraction using different solvents viz. water, methanol and ethanol either alone or in combination with water. the extraction yield, antioxidant activity (dpph and abts inhibition) and total phenolic contents were evaluated. highest yield was obtained from 50 % ethanol: 50 % water (16.3 ± 1.99 %). the dpph and abts inhibition activity was found to be the highest for methanol and 70 % ethanol: 30 % water extract (79.5 ± 6.5; 94.6 ± 6.10), res ... | 2014 | 25477693 |
| combining urban wastewater treatment with biohydrogen production--an integrated microalgae-based approach. | the aim of the present work was the simultaneous treatment of urban wastewater using microalgae and the energetic valorization of the obtained biomass. chlorella vulgaris (cv), scenedesmus obliquus (sc) and a naturally occurring algal consortium c (consc) were grown in an urban wastewater. the nutrient removals were quite high and the treated water fits the legislation (pt dec-lei 236/98) in what concerns the parameters analysed (n, p, cod). after nutrient depletion the microalgae remained two m ... | 2015 | 25453433 |
| use of aiia gene amplification for ahl-lactonase production from endophytic bacterium enterobacter species. | ahl-lactonase has gained renewed interest due to biotechnological applications such as antiquorum sensing, antibiofilm strategies, biofouling, etc. in our study, the production of ahl-lactonase from endophytic bacteria enterobacter aerogenes vt66 was optimized by response surface methodology (rsm) using central composite design (ccd) for four different cultural conditions. the relative activity of ahl-lactonase was correlated with amplification of aiia homologous gene amplification with respect ... | 2015 | 25451751 |
| cytotoxic, antibacterial and antioxidant activities of extracts of the bark of melia azedarach (china berry). | nature provides a variety of drugs and medicinal agents derived from plants. this study was conducted to determine antimicrobial, antioxidant and cytotoxic activities of extracts of melia azedarach bark with methanol/water (9:1 v/v), chloroform, butanol, hexane, water and ethyl acetate. for the determination of the antimicrobial activities, the agar well diffusion method was employed. cytotoxicity was studied by brine shrimp lethality assay; antioxidant activities were measured using 1,1-dipheny ... | 2015 | 25426766 |
| effects of eliminating pyruvate node pathways and of coexpression of heterogeneous carboxylation enzymes on succinate production by enterobacter aerogenes. | lowering the ph in bacterium-based succinate fermentation is considered a feasible approach to reduce total production costs. newly isolated enterobacter aerogenes strain aj110637, a rapid carbon source assimilator under weakly acidic (ph 5.0) conditions, was selected as a platform for succinate production. our previous work showed that the δadhe/pck strain, developed from aj110637 with inactivated ethanol dehydrogenase and introduced actinobacillus succinogenes phosphoenolpyruvate carboxykinase ... | 2015 | 25416770 |
| authors reply to letter to the editor--in response to: "bhat ss, undrakonda v, mukhopadhyay c, parmar pv. outbreak of multidrug-resistant acute postoperative endophthalmitis due to enterobacter aerogenes". | | 2015 | 25356851 |
| in response to: "bhat ss, undrakonda v, mukhopadhyay c, parmar pv. outbreak of multidrug-resistant acute postoperative endophthalmitis due to enterobacter aerogenes.". | | 2015 | 25356796 |
| multiplex real-time pcr probe-based for identification of strains producing: oxa48, vim, kpc and ndm. | the spread of multi-resistant enterobacteria, particularly carbapenem-resistant enterobacteriaceae (cre), in both community and hospital settings is a global problem. the phenotypic identification of cre is complex, occasionally inconclusive and time consuming. however, commercially available molecular assays are very expensive, and many do not allow the simultaneous identification of all genetic markers of resistance that have been recognised in cre (bla kpc, bla oxa-48, bla vim and bla ndm). t ... | 2014 | 25154795 |
| distribution of free-living amoebae in a treatment system of textile industrial wastewater. | free-living amoebae have been found in soil, air and a variety of aquatic environments, but few studies have been conducted on industrial wastewater and none on wastewater from the textile industry. the aim of this study was to determine the presence and distribution of free-living amoebae in a biological treatment system that treats textile industrial wastewater. samples were taken from input, aeration tank, sedimentation tank and output. samples were centrifuged at 1200g for 15min, the sedimen ... | 2014 | 25072828 |
| chemical composition, antibacterial and antioxidant profile of essential oil from murraya koenigii (l.) leaves. | this study is designed to extract and examine chemical composition, antimicrobial and antioxidant activity of the hydro-distillated essential oil of murraya koenigii leaves from the south region of tamilnadu, india. matherials and methods: gas chromatography (gc) and gas chromatography-mass spectrometry (gc-ms) analysis of the essential oil result was indicates the 33 different compounds representing 97.56 % of the total oil. | 2014 | 25050318 |
| partial purification and characterization of a novel histidine decarboxylase from enterobacter aerogenes dl-1. | histidine decarboxylase (hdc) from enterobacter aerogenes dl-1 was purified in a three-step procedure involving ammonium sulfate precipitation, sephadex g-100, and deae-sepharose column chromatography. the partially purified enzyme showed a single protein band of 52.4 kd on sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). the optimum ph for hdc activity was 6.5, and the enzyme was stable between ph 4 and 8. enterobacter aerogenes hdc had optimal activity at 40°c and retained ... | 2015 | 25036745 |
| study of the role of anaerobic metabolism in succinate production by enterobacter aerogenes. | succinate is a core biochemical building block; optimizing succinate production from biomass by microbial fermentation is a focus of basic and applied biotechnology research. lowering ph in anaerobic succinate fermentation culture is a cost-effective and environmentally friendly approach to reducing the use of sub-raw materials such as alkali, which are needed for neutralization. to evaluate the potential of bacteria-based succinate fermentation under weak acidic (ph <6.2) and anaerobic conditio ... | 2014 | 24962116 |
| persistence of nosocomial bacteria on 2 biocidal fabrics based on silver under conditions of high relative humidity. | the survival of pathogenic microorganism on fabrics in the health care environment has a important role in nosocomial infections. the use of biocidal fabrics and surfaces could reduce the prevalence of the microorganisms in the hospital environment. | 2014 | 24930955 |
| biohydrogen and polyhydroxyalkanoate co-production by enterobacter aerogenes and rhodobacter sphaeroides from calophyllum inophyllum oil cake. | the feasibility of coupled biohydrogen and polyhydroxyalkanoate production by enterobacter aerogenes and rhodobacter sphaeroides using calophyllum inophyllum oil cake was studied under dark and photo fermentation conditions. the utilization of a non-edible acidic oil cake (c. inophyllum), and exploitation of a modified minimal salt media led to reduction in the cost of media. cost of fermentation is reduced by implementation of alternate dark-photo fermentative periods and through the use of a c ... | 2014 | 24859207 |
| abiotic process for fe(ii) oxidation and green rust mineralization driven by a heterotrophic nitrate reducing bacteria (klebsiella mobilis). | green rusts (grs) are mixed fe(ii)-fe(iii) hydroxides with a high reactivity toward organic and inorganic pollutants. grs can be produced from ferric reducing or ferrous oxidizing bacterial activities. in this study, we investigated the capability of klebsiella mobilis to produce iron minerals in the presence of nitrate and ferrous iron. this bacterium is well-known to reduce nitrate using an organic carbon source as electron donor but is unable to enzymatically oxidize fe(ii) species. during in ... | 2014 | 24605878 |
| pleural effusion due to streptococcus milleri: case descriptions. | in this study we analyzed the characteristics of patients with pleural effusion secondary to streptococcus milleri studied retrospectively between january and march 2013 and found seven patients with a mean age of 60 years, 43% of which were smokers and 57% with a drinking habit. the most common associated factors were alcoholism, previous pneumonia and diabetes. other bacteria were identified as enterobacter aerogenes, bacteroides and prevotella intermedia capillosus in two patients. the mean d ... | 2014 | 24439468 |
| biosensing and bioremediation of cr(vi) by cell free extract of enterobacter aerogenes t2. | hexavalent chromium or cr(vi) enters the environment through several anthropogenic activities and it is highly toxic and carcinogenic. hence it is required to be detected and remediated from the environment. in this study, low-cost and environment-friendly methods of biosensing and bioremediation of cr(vi) have been proposed. crude cell free extract (cfe) of previously isolated enterobacter aerogenes t2 (gu265554; nii 1111) was prepared and exploited to develop a stable biosensor for direct esti ... | 2014 | 24410691 |
| biosynthesis of silver nanoparticles from marine seaweed sargassum cinereum and their antibacterial activity. | seaweed extracts of sargassum cinereum was used as a reducing agent in the eco-friendly extracellular synthesis of silver nanoparticles from an aqueous solution of silver nitrate (agno3). high conversion of silver ions to silver nanoparticles was achieved with a reaction temperature of 100(°) and a seaweed extract concentration of 10% with a residential time of 3 h. formation of silver nanoparticles was characterised by spectrophotometry and the scanning electron microscope. the average particle ... | 2013 | 24403664 |
| inactivation of enterobacter aerogenes in reconstituted skim milk by high- and low-frequency ultrasound. | the inactivation of enterobacter aerogenes in skim milk using low-frequency (20khz) and high-frequency (850khz) ultrasonication was investigated. it was found that low-frequency acoustic cavitation resulted in lethal damage to e. aerogenes. the bacteria were more sensitive to ultrasound in water than in reconstituted skim milk having different protein concentrations. however, high-frequency ultrasound was not able to inactivate e. aerogenes in milk even when powers as high as 50w for 60min were ... | 2014 | 24394387 |
| antibacterial attributes of apigenin, isolated from portulaca oleracea l. | the flavonoid apigenin was isolated from aerial part of p. oleracea l. the dried sample of plant was powdered and subjected to soxhlet extractor by adding 80 ml of ethanol : water (70 : 30). the extract was centrifuged at 11000 rpm for 30 min; supernatant was taken for further use. the fraction was concentrated and subjected to ptlc. the r f value of isolated apigenin was calculated (0.82). purified material was also subjected to its ir spectra, lc-ms, nmr, and hplc for structural elucidation. t ... | 2014 | 26904730 |
| first detection and characterization of an oxa-48-producing enterobacter aerogenes isolate. | | 2014 | 24355605 |
| risk factors and treatment outcomes of bloodstream infection caused by extended-spectrum cephalosporin-resistant enterobacter species in adults with cancer. | treatment of enterobacter infection is complicated due to its intrinsic resistance to cephalosporins. medical records of 192 adults with cancer who had enterobacter bacteremia were analyzed retrospectively to evaluate the risk factors for and the treatment outcomes in extended-spectrum cephalosporin (esc)-resistant enterobacter bacteremia in adults with cancer. the main outcome measure was 30-day mortality. of the 192 patients, 53 (27.6%) had bloodstream infections caused by esc-resistant entero ... | 2014 | 24321352 |
| hepatoblastoma in a 15-month-old female with trisomy 13. | trisomy 13 (t13) is a rare autosomal aneuploidy. greater than 90% of patients die during the first year of life. malignancies reported in association with t13 include two cases of wilms tumor and one case of pilocytic astrocytoma. there is no previous report of hepatoblastoma in patients with t13. we report a unique case of hepatoblastoma in a 15-month-old female with constitutional t13. our patient was born at 38 weeks gestation and was noted to have phenotypic features consistent with t13. gen ... | 2014 | 24311532 |
| biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation. | hydrogen production from sugarcane bagasse (scb) by integrating dark-fermentation by enterobacter aerogenes mtcc 2822 and photo-fermentation by rhodopseudomonas bhu 01 was investigated. the scb was hydrolysed by sulphuric acid and the hydrolysate detoxified by passing through adsorbent resin column (amberlite xad-4) to remove the inhibitory furfural, and subjected to dark-fermentation. the cellulosic residue from acid hydrolysis was hydrolysed by the new isolate cellulomonas fimi to release suga ... | 2014 | 24291314 |
| hospital clonal dissemination of enterobacter aerogenes producing carbapenemase kpc-2 in a chinese teaching hospital. | carbapenems are first-line agents for the treatment of serious nosocomial infections caused by multidrug-resistant enterobacteriaceae. however, resistance to carbapenems has increased dramatically among enterobacteriaceae in our hospital. in this study, we report clonal dissemination caused by carbapenem-resistant enterobacter aerogenes (crea). in 2011, crea was identified from 12 patients admitted to the neurosurgical ward. all 12 clinical isolates were non-susceptible to cefotaxime, ceftazidim ... | 2014 | 24273320 |
| immobilization of the enzyme gpdq on magnetite nanoparticles for organophosphate pesticide bioremediation. | annually thousands of people die or suffer from organophosphate (pesticide) poisoning. in order to remove these toxic compounds from the environment, the use of enzymes as bioremediators has been proposed. we report here a ser127ala mutant based on the enzyme glycerophosphodiesterase (gpdq) from enterobacter aerogenes. the mutant, with improved metal binding abilities, has been immobilized using glutaraldehyde on pamam dendrimer-modified magnetite nanoparticles. the immobilized system was charac ... | 2014 | 24239906 |
| biodegradation of 2-methylquinoline by enterobacter aerogenes tj-d isolated from activated sludge. | bacterial strain enterobacter aerogenes tj-d capable of utilizing 2-methylquinoline as the sole carbon and energy source was isolated from acclimated activated sludge under denitrifying conditions. the ability to degrade 2-methylquinoline by e. aerogenes tj-d was investigated under denitrifying conditions. under optimal conditions of temperature (35 degrees c) and initial ph 7, 2-methylquinoline of 100 mg/l was degraded within 176 hr. the degradation of 2-methylquinoline by e. aerogenes tj-d cou ... | 2013 | 24218841 |
| co-fermentation of carbon sources by enterobacter aerogenes atcc 29007 to enhance the production of bioethanol. | we investigated the enhancement of bioethanol production in enterobacter aerogenes atcc 29007 by co-fermentation of carbon sources such as glycerol, glucose, galactose, sucrose, fructose, xylose, starch, mannitol and citric acid. biofuel production increases with increasing growth rate of microorganisms; that is why we investigated the optimal growth rate of e. aerogenes atcc 29007, using mixtures of different carbon sources with glycerol. e. aerogenes atcc 29007 was incubated in media containin ... | 2014 | 24185706 |
| spectroscopic and mechanistic studies of dinuclear metallohydrolases and their biomimetic complexes. | an enhanced understanding of the metal ion binding and active site structural features of phosphoesterases such as the glycerophosphodiesterase from enterobacter aerogenes (gpdq), and the organophosphate degrading agent from agrobacterium radiobacter (opda) have important consequences for potential applications. coupled with investigations of the metalloenzymes, programs of study to synthesise and characterise model complexes based on these metalloenzymes can add to our understanding of structur ... | 2014 | 24135968 |
| infection due to colistin-resistant enterobacteriacae in critically-ill patients. | this study was conducted in response to the rising incidence of drug resistance observed in the intensive care unit (icu) of king fahad medical city. | 2013 | 24129623 |
| biodegradation of glycerol using bacterial isolates from soil under aerobic conditions. | glycerol, a non-biodegradable by-product during biodiesel production is a major concern to the emerging biodiesel industry. many microbes in natural environments have the ability to utilize glycerol as a sole carbon and energy source. the focus of this study was to screen for microorganisms from soil, capable of glycerol utilization and its conversion to value added products such as ethanol and 1,3-propanediol (1,3-pdo). twelve bacterial isolates were screened for glycerol utilization ability in ... | 2014 | 24117087 |
| development of a practical complete-kill assay to evaluate anti-acanthamoeba drugs. | acanthamoeba keratitis is a debilitating eye disease that requires effective topical drug therapy. currently, there is no standard in vitro test to evaluate anti-acanthamoeba drugs. | 2013 | 24077460 |
| outbreak of multidrug-resistant acute postoperative endophthalmitis due to enterobacter aerogenes. | to report the clinical features, management, and outcome of 7 cases of culture-proven multidrug-resistant enterobacter postoperative endophthalmitis following cataract surgery. | 2014 | 24063692 |
| extraction, gas chromatography-mass spectrometry analysis and screening of fruits of terminalia chebula retz. for its antimicrobial potential. | terminalia chebula is called the "king of medicines" in tibet and is always listed first in the ayurvedic meteria medica because of its extraordinary powers of healing. | 2013 | 23901211 |
| analysis of a soluble (ured:uref:ureg)2 accessory protein complex and its interactions with klebsiella aerogenes urease by mass spectrometry. | maturation of the nickel-containing urease of klebsiella aerogenes is facilitated by the ured, uref, and ureg accessory proteins along with the uree metallo-chaperone. a fusion of the maltose binding protein and ured (mbp-ured) was co-isolated with uref and ureg in a soluble complex possessing a (mbpured: uref:ureg)2 quaternary structure. within this complex a uref:uref interaction was identified by chemical cross-linking of the amino termini of its two uref protomers, as shown by mass spectrome ... | 2013 | 23797863 |
| engineered enterobacter aerogenes for efficient utilization of sugarcane molasses in 2,3-butanediol production. | sugarcane molasses is considered to be a good carbon source for biorefinery due to its high sugar content and low price. sucrose occupies more than half of the sugar in the molasses. enterobacter aerogenes is a good host strain for 2,3-butanediol production, but its utilization of sucrose is not very efficient. to improve sucrose utilization in e. aerogenes, a sucrose regulator (scrr) was disrupted from the genomic dna. the deletion mutation increased the sucrose consumption rate significantly w ... | 2013 | 23644066 |
| determination and frying loss of histamine in striped marlin fillets implicated in a foodborne poisoning. | an incident of foodborne poisoning causing illness in 67 victims due to ingestion of fried fish fillets occurred in june 2011, in southern taiwan. of the five suspected fish fillets, one fried sample contained 62.0 mg/100 g and one raw sample contained 89.6 mg/100 g histamine, levels which are greater than the potential hazard action level (50 mg/100 g) in most illness cases. given the allergy-like symptoms of the victims and the high histamine content in the suspected fish samples, this foodbor ... | 2013 | 23643129 |
| in vivo transmission of a plasmid containing the kpc-2 gene in a single patient. | here we describe a case of in vivo horizontal interspecies transmission of a kpc-2-producing plasmid from a klebsiella pneumoniae to an enterobacter aerogenes strain in the same patient. the patient's gut flora initially contained a carbapenem-susceptible e. aerogenes strain and 10 days after admission a kpc-2-positive k. pneumoniae. three months after admission, a kpc-2-positive e. aerogenes was identified in fecal surveillance cultures. this isolate was isogenic with the initial e. aerogenes a ... | 2013 | 27873604 |
| impact of the prone position in an animal model of unilateral bacterial pneumonia undergoing mechanical ventilation. | the prone position (pp) has proven beneficial in patients with severe lung injury subjected to mechanical ventilation (mv), especially in those with lobar involvement. we assessed the impact of pp on unilateral pneumonia in rabbits subjected to mv. | 2013 | 23416383 |
| optimization of cultural conditions for conversion of glycerol to ethanol by enterobacter aerogenes s012. | the aim of this research is to optimize the cultural conditions for the conversion of glycerol to ethanol by enterobacter aerogenes s012. taguchi method was used to screen the cultural conditions based on their signal to noise ratio (sn). temperature (°c), agitation speed (rpm) and time (h) were found to have the highest influence on both glycerol utilization and ethanol production by the organism while ph had the lowest. full factorial design, statistical analysis, and regression model equation ... | 2013 | 23388539 |
| search for new tools to combat gram-negative resistant bacteria among amine derivatives of 5-arylidenehydantoin. | a series of amine-alkyl derivatives of 5-arylidenehydantoin 3-21 was evaluated for their ability to improve antibiotic effectiveness in two strains of gram-negative enterobacter aerogenes: the reference strain (atcc-13048) and the chloramphenicol-resistant derivative over-producing the acrab-tolc efflux pump (cm-64). three antibiotics, chloramphenicol, nalidixic acid and sparfloxacin were used as markers of efflux pump activity. new compounds (5-16) were obtained within 3-4 step synthesis using ... | 2013 | 23218781 |
| bactericidal effect of s-nitrosothiols against clinical isolates from keratitis. | the purpose of this study was to evaluate the antimicrobial activity of two nitric oxide donors, ie, s-nitrosoglutathione (gsno) and s-nitroso-n-acetylcysteine (snac), against clinical isolates from patients with infectious keratitis. | 2012 | 23204833 |
| antibacterial activities of the extracts, fractions and compounds from dioscorea bulbifera. | dioscorea bulbifera is an african medicinal plant used to treat microbial infections. in the present study, the methanol extract, fractions (dbb1 and dbb2) and six compounds isolated from the bulbils of d. bulbifera, namely bafoudiosbulbins a (1), b (2), c (3), f (4), g (5) and 2,7-dihydroxy-4-methoxyphenanthrene (6), were tested for their antimicrobial activities against mycobacteria and gram-negative bacteria involving multidrug resistant (mdr) phenotypes expressing active efflux pumps. | 2012 | 23176193 |
| the rhizome of the multidrug-resistant enterobacter aerogenes genome reveals how new "killer bugs" are created because of a sympatric lifestyle. | here, we sequenced the 5,419,609 bp circular genome of an enterobacter aerogenes clinical isolate that killed a patient and was resistant to almost all current antibiotics (except gentamicin) commonly used to treat enterobacterial infections, including colistin. genomic and phylogenetic analyses explain the discrepancies of this bacterium and show that its core genome originates from another genus, klebsiella. atypical characteristics of this bacterium (i.e., motility, presence of ornithine deca ... | 2013 | 23071100 |
| klebsiella michiganensis sp. nov., a new bacterium isolated from a tooth brush holder. | isolate w14(t) recovered from a household tooth brush holder was found to be gram-negative, a facultative anaerobic, non-motile, capsulated, and a non-endospore-forming straight rod. based on phylogenetic analysis with 16s rrna gene sequence, isolate w14(t) was affiliated to the genus klebsiella. the closest phylogenetic relative was k. oxytoca with 99 % similarity in the 16s rrna gene sequence. the major whole-cell fatty acids were c(16:0) (31.23 %), c(18:1ω6c)/c(18:1ω7c) (21.10 %), and c(16:1ω ... | 2013 | 23053492 |
| biogenic amine content, histamine-forming bacteria, and adulteration of pork in tuna sausage products. | twenty-five tuna sausage products were purchased from retail markets in taiwan. the rates of occurrence of biogenic amines, histamine-forming bacteria, and adulteration by pork and poultry were determined. the average content of various biogenic amines in all tested samples was less than 2.0 mg/100 g (<0.05 to 1.85 mg/100 g). thirteen histamine-producing bacterial strains isolated from tested samples produced 12.1 to 1,261 ppm of histamine in trypticase soy broth supplemented with 1.0% l-histidi ... | 2012 | 23043830 |