| chemical, microbial and sensory changes during the anaerobic cold storage of beef inoculated with a homofermentative lactobacillus sp. or a leuconostoc sp. | slices of beef were inoculated with about 3.5 log cfu/cm2 of lactobacillus sp. 93 smricc 235 (homofermentative) or leuconostoc sp. 89 smricc 189 and stored in 5% co2 + 95% n2 at 4 degrees c. the microbial, chemical (glucose, l-lactate, d-lactate, acetate, formate, ethanol, h2s) and sensory changes of the beef slices were studied. for beef inoculated with lactobacillus sp. 93 the flavour score started to decrease when the maximum bacterial count was reached. leuconostoc sp. 89 caused a rapid decr ... | 1992 | 1622763 |
| lung protein leakage in respiratory failure induced by a hybridoma making monoclonal antibody to the hydrophobic surfactant-associated polypeptide sp-b. | adult mice were inoculated intraperitoneally with a hybridoma (8b5e) making monoclonal antibody to the porcine surfactant-associated polypeptide sp-b; this antibody cross-reacts with the corresponding polypeptide in the mouse surfactant system. respiratory failure, developing 7-9 days after inoculation, was associated with a decrease in lung-thorax compliance determined during artificial ventilation, and an increase in the amount of protein including the specific antibody in lung lavage fluid. t ... | 1992 | 1622844 |
| cholangiohepatitis in a dog. | cholangiohepatitis was diagnosed in a dog with a 4-day history of anorexia, vomiting, fever, and icterus. additional findings included signs of depression, dehydration, hepatosplenomegaly, and abdominal discomfort. exploratory laparotomy was performed, and specimens of liver, spleen, and bile were obtained. histologic evaluation of liver and spleen revealed acute, suppurative cholangio-hepatitis and splenitis, respectively. cultures of liver and bile yielded klebsiella sp. the dog responded to r ... | 1992 | 1624352 |
| macrolactams: two novel homologous series of compounds produced by actinomadura sp. scc 1778. | eight antifungal compounds were identified from the fermentation of actinomadura sp. scc 1778. this culture produces four homologous compounds (c22h42n2o5 approximately c25h48n2o5) containing the sugar, mycosamine, and four homologous compounds (c22h42n2o5 approximately c25h48n2o5) containing the sugar, 3,6-dideoxy-3-amino-l-talopyranose. five of the compounds identified were novel macrolactams. all these compounds exhibit antifungal activity against candida spp. with geometric mean mics ranging ... | 1992 | 1624365 |
| the squalestatins, novel inhibitors of squalene synthase produced by a species of phoma. i. taxonomy, fermentation, isolation, physico-chemical properties and biological activity. | during the screening of fungi for inhibitors of squalene synthase, phoma sp. c2932 was found to produce three structurally related novel inhibitors. these compounds, designated the squalestatins, exhibited potent activity against both mammalian (rat liver) and fungal (candida albicans) squalene synthase. furthermore, they also had broad spectrum in vitro antifungal activity. | 1992 | 1624366 |
| ws-7338, new endothelin receptor antagonists isolated from streptomyces sp. no. 7338. iii. structures of ws-7338 a, b, c and d and total synthesis of ws-7338 b. | | 1992 | 1624381 |
| acetyl-coenzyme a synthesis from methyltetrahydrofolate, co, and coenzyme a by enzymes purified from clostridium thermoaceticum: attainment of in vivo rates and identification of rate-limiting steps. | many anaerobic bacteria fix co2 via the acetyl-coenzyme a (coa) (wood) pathway. carbon monoxide dehydrogenase (codh), a corrinoid/iron-sulfur protein (c/fe-sp), methyltransferase (metr), and an electron transfer protein such as ferredoxin ii play pivotal roles in the conversion of methyltetrahydrofolate (ch3-h4folate), co, and coa to acetyl-coa. in the study reported here, our goals were (i) to optimize the method for determining the activity of the synthesis of acetyl-coa, (ii) to evaluate how ... | 1992 | 1624454 |
| chlorosis induced by nutrient deprivation in synechococcus sp. strain pcc 7942: not all bleaching is the same. | cell coloration changes from normal blue-green to yellow or yellow-green when the cyanobacterium synechococcus sp. strain pcc 7942 is deprived of an essential nutrient. we found that this bleaching process (chlorosis) in cells deprived of sulfur (s) was similar to that in cells deprived of nitrogen (n), but that cells deprived of phosphorus (p) bleached differently. cells divided once after n deprivation, twice after s deprivation, and four times after p deprivation. chlorophyll (chl) accumulati ... | 1992 | 1624459 |
| high-performance liquid chromatography of corynomycolic acids as a tool in identification of corynebacterium species and related organisms. | a high-performance liquid chromatography (hplc) study of 307 strains of corynebacterium species and related taxa revealed that strains classified as "corynebacterium aquaticum"; "corynebacterium asperum"; and centers for disease control (cdc) groups 1, 2, a-3, a-4, a-5, b-1, b-3, e, f-2, and i-2 as well as some unidentified coryneforms do not contain any corynomycolic acids; therefore, they should not be included in the genus corynebacterium. such an hplc method of identification permitted the c ... | 1992 | 1624556 |
| seroepidemiology status of nepalese against hemolytic streptococcal infection in katmandu. | immune status of apparently healthy nepalese against hemolytic streptococci was studied by measuring antibodies to various streptococcal antigens such as streptolysin-o (so), streptokinase (sk), deoxyribonuclease-b (dn-b) and streptococcal polysaccharide (sp). the normal range of anti-streptolysin-o (aso), anti-streptokinase (ask), anti-deoxyribonuclease-b (adn-b) and anti-streptococcus polysaccharide (asp) was 60-480 (t.u.), 40-1024 (titer), 60-1280 (titer) and less than 2-128 (unit), respectiv ... | 1992 | 1624820 |
| [pneumonia caused by moraxella subgenus moraxella sp]. | moraxella subgenus moraxella sp. was isolated in pure culture from the sputum of a 43-year-old male with pneumonia and congestive heart failure due to idiopathic dilated cardiomyopathy. in this case, we concluded that the patient's bacterial pneumonia was caused by m. (m.) sp. based on a gram stain of the sputum smear and bacterial findings, increased wbc count, and elevated crp. a chest x-ray revealed right middle, and left upper and middle lobe infiltrates. this moraxella strain produced a bro ... | 1992 | 1624833 |
| effects of ovine conceptus secretory proteins and progesterone on oxytocin-stimulated endometrial production of prostaglandin and turnover of inositol phosphate in ovariectomized ewes. | this study examined the effects of progesterone and intrauterine injection of ovine conceptus secretory proteins (ocsp) on endometrial responsiveness to oxytocin. twelve ewes were ovariectomized on day 4 of the cycle (oestrus = day 0) and assigned in a 2 x 2 factorial arrangement, to receive either 1.5 mg ovine serum proteins (sp) or ocsp containing 25 micrograms ovine trophoblast protein 1 (otp-1) (by radioimmunoassay) in 1.5 mg total protein into each uterine horn, via catheters, twice a day o ... | 1992 | 1625235 |
| ectoparasitic mites (acari) of sympatric brazilian free-tailed bats and big brown bats in alabama. | seven species of mites were recovered from 133 brazilian free-tailed bats, tadarida brasiliensis, and 94 big brown bats, eptesicus fuscus, from february through november 1990 in colonies that shared roosting space in east-central alabama. the macronyssid chiroptonyssus robustipes (ewing) was the most common mite on t. brasiliensis (964 mites, 87% of bats infested) and on e. fuscus (109 mites, 29% of bats infested). however, c. rubustipes normally is a specific parasite of t. brasiliensis. the ma ... | 1992 | 1625301 |
| facultative myiasis by megaselia sp. (diptera: phoridae) in texas: a case report. | facultative myiasis caused by larval megaselia sp. is reported in a human in texas. on two occasions during 24 h, the youth experienced a "crawling sensation deep in [his] throat" which caused him to cough, expelling a living megaselia sp. larva. the site of the infestation was presumed to be the sinuses or upper respiratory tract. the symptoms resolved upon expulsion of the larvae, and no additional complications were observed in the patient. | 1992 | 1625307 |
| cloacal flora isolated from wild black-bellied whistling ducks (dendrocygna autumnalis) in laguna la nacha, mexico. | cloacal swabs from 110 adult black-bellied whistling ducks trapped at laguna la nacha, tamaulipas, mexico, were cultured to determine the prevalence of normal and potentially pathogenic bacteria. twenty-five gram-negative enterobacteria and four gram-positive cocci were isolated. the most common isolates included escherichia coli (54%), staphylococcus spp. (29%), streptococcus spp. (22%), aeromonas hydrophila (15%) enterobacter cloacae (14%), and micrococcus sp. (14%). the implications of whistl ... | 1992 | 1627117 |
| cloning and nucleotide sequence of the vibrio proteolyticus aminopeptidase gene. | the gene encoding the vibrio proteolyticus aminopeptidase was cloned and sequenced and its amino acid sequence was deduced. the gene encodes a 54 kda protein, larger than the previously reported size of 30 kda for the purified aminopeptidase. sequence alignments revealed a 43-45% homology with two other vibrio sp. extracellular proteinases. | 1992 | 1627651 |
| acetylornithine deacetylase, succinyldiaminopimelate desuccinylase and carboxypeptidase g2 are evolutionarily related. | the nucleotide (nt) sequence of the escherichia coli arge gene, encoding the acetylornithine deacetylase (ao) subunit, has been established and corresponds to a 43-kda (m(r) 42,320) polypeptide. the enzyme has been purified to near homogeneity and it appears to be a dimer consisting of two 43-kda subunits. the amino acid sequence deduced from the nt sequence was compared to that of the subunit of e. coli succinyldiaminopimelate desuccinylase (the dape gene product involved in the diaminopimelate ... | 1992 | 1628835 |
| cloning and primary structure of the wide-spectrum amidase from brevibacterium sp. r312: high homology to the amie product from pseudomonas aeruginosa. | a brevibacterium sp. r312 dna fragment encoding the wide-spectrum amidase (ec 3.5.1.4) has been cloned and sequenced, using limited amino acid (aa) sequence information obtained from the purified enzyme. the deduced aa sequence showed more than 80% strict identity with the pseudomonas aeruginosa aliphatic amidase, the product of the amie gene, suggesting a horizontal transfer of the gene during evolution between gram+ and gram- bacteria. | 1992 | 1628849 |
| analysis of proenkephalin a, proopiomelanocortin and protachykinin neuropeptides in human lumbar cerebrospinal fluid by reversed-phase high-performance liquid chromatography, radioimmunoassay and enzymolysis. | a comprehensive high-performance liquid chromatographic, radioimmunoassay, and enzymatic degradation scheme has been developed to analyze several intact neuropeptides and the corresponding peptides created by in vivo enzymolysis of precursors to study neuropeptides in human lumbar cerebrospinal fluid (csf) and to test the hypothesis that defects in the metabolism (synthesis, degradation) of neuropeptide precursors, neuropeptides, and metabolites play a role in low back pain. csf samples were obt ... | 1992 | 1629297 |
| control of trigeminal motoneurons from the cerebellar interpositus nucleus of the guinea pig. | 1. effects of stimulation of the cerebellar interpositus nucleus (ipn) on jaw reflexes and trigeminal motoneurons were studied along with the route responsible for the effects in ketamine-anesthetized guinea pigs. 2. stimulation in the ipn evoked a bilateral, ipsilaterally dominant, short-latency reciprocal effect on jaw reflexes: a depression of the jaw-closing masseteric reflex and a facilitation of the jaw-opening digastric reflex. this reciprocal phase was followed by a non-reciprocal facili ... | 1992 | 1629762 |
| functional tolerance of the human immunodeficiency virus type 1 envelope signal peptide to mutations in the amino-terminal and hydrophobic regions. | we demonstrated that the leader sequence of the human immunodeficiency virus type 1 envelope functions as signal peptide (sp) despite low scoring in a prediction program. as expected for sp, the hydrophobic core (hc) is essential, and no other sequence could compensate for hc deletion. contrary to other sps, major substitutions in the hc, such as introduction of basic, polar, or alpha-helix-breaking residues, still allowed efficient translocation and glycosylation. also, extensive deletions or s ... | 1992 | 1629966 |
| nosocomial urinary tract infections at a pediatric hospital. | although the epidemiology of nosocomial urinary tract infections (nutis) is well-described in the adult population, there is little information on children. we therefore reviewed all our patients with nutis, to determine their age, underlying diagnoses, microorganisms involved, use of catheters and secondary bacteremias. during a 2-year period at our institution, nutis accounted for 10% of all nosocomial infections (0.8 infections/100 admissions). of 44,948 patients admitted during this period 0 ... | 1992 | 1630854 |
| introducing mutations into the single-copy chromosomal 23s rrna gene of the archaeon halobacterium halobium by using an rrna operon-based transformation system. | a vector-transformation system is described that permits replacement of a portion of the single rrna operon of the archaeon halobacterium halobium with a homologous fragment from a vector-borne gene. the vector construct contains three functional sections: (i) an entire h. halobium rrna operon with two selective mutations in the 23s rrna gene, the substitutions of a----g at position 1159 conferring resistance to thiostrepton and c----u at position 2471 conferring resistance to anisomycin; (ii) t ... | 1992 | 1631155 |
| removal of copper ion by pseudomonas spp. | copper-resistant pseudomonas sp. 41y, pseudomonas pseudomallei 13-1 and pseudomonas aeruginosa 7 were used in the present study. when the latter two organisms were added to copper-containing 1/3 strength tryptic soy broth, more than 99.5% of the copper ion was removed from the medium within 24 h. if copper solution was added to hog waste slurry, a reduction in the copper ion concentration could be detected only when the added bacteria started to grow in it, whereas in a mineral medium supplement ... | 1992 | 1631243 |
| [brucellar sacroiliitis. a detailed review with an analysis of treatment efficacy]. | all patients diagnosed of brucellosis in 4 regional hospitals during a period of nine years were reviewed, in an attempt to evaluate the efficacy of different antimicrobic regimens and also the clinical features. evolution and complications of brucellar sacroiliitis. of a total of 548 patients, 12% had been diagnosed of sacroiliitis and, of theme, 49 had complete data available with efficacy of different treatments, the time elapsed until fever and pain ceased was evaluated, together with initia ... | 1992 | 1631354 |
| quantification of lipopolysaccharides in human bile with or without gram-negative bacteria. | lipopolysaccharide (lps) was assayed in 78 samples of human common-duct bile, obtained at endoscopic retrograde cholangiography. the lps was assayed by a chromogenic limulus amebocyte lysate (lal) test, after dilution of bile samples in heparinized plasma and inactivation of inhibitors. the assay was not influenced by other biliary constituents, as demonstrated by the recovery of standards. bile pigments did not influence the results. the lal test was positive in 60 of the samples, 59 of which h ... | 1992 | 1631492 |
| identification of oxidatively modified lipids in atherosclerotic lesions of human aortas. | the oxygenated lipids of human aortas with atherosclerotic lesions were analysed by rp-, sp- and chiral phase-hplc and compared with those obtained after treatment of human ldl with pure 15-lipoxygenase (lox) of rabbit reticulocytes. the data suggest an indirect role of 15-lox in the atherogenesis in aortas. | 1992 | 1632313 |
| high-performance liquid chromatographic determination of catecholamines and their precursor and metabolites in human urine and plasma by postcolumn derivatization involving chemical oxidation followed by fluorescence reaction. | a high-performance liquid chromatographic method for the determination of catecholamines and their precursor and metabolites [amino compounds (norepinephrine, epinephrine, dopamine, normetanephrine, metanephrine, 3-methoxytyramine, and l-dopa), acidic compounds (3,4-dihydroxyphenylacetic acid, vanillyl-mandelic acid, and homovanillic acid), and alcoholic compound [4-hydroxy-3-methoxyphenyl)ethylene glycol)] in human urine and plasma. urine and plasma samples deproteinized with perchloric acid in ... | 1992 | 1632497 |
| ferredoxin and flavodoxin from the cyanobacterium synechocystis sp pcc 6803. | the unicellular cyanobacterium synechocystis sp pcc 6803 is capable of synthesizing two different photosystem-i electron acceptors, ferredoxin and flavodoxin. under normal growth conditions a [2fe-2s] ferredoxin was recovered and purified to homogeneity. the complete amino-acid sequence of this protein was established. the isoelectric point (pi = 3.48), midpoint redox potential (em = -0.412 v) and stability under denaturing conditions were also determined. this ferredoxin exhibits an unusual ele ... | 1992 | 1633177 |
| the photosystem i-like p840-reaction center of green s-bacteria is a homodimer. | an operon encoding the p840 reaction center of chlorobium limicola f.sp.thiosulfatophilum has been cloned and sequenced. it contains two structural genes coding for proteins of 730 and 232 amino acids. the first protein resembles the large subunits of the photosystem i (ps i) reaction center. putative binding elements for the primary donor, p840 in chlorobium and p700 in ps i and for the acceptors a(o), a(1) and fes-center x are conserved. the second protein is related to the ps i subunit carryi ... | 1992 | 1633181 |
| molecular cloning of a glucoamylase gene from a thermophilic clostridium and kinetics of the cloned enzyme. | clostridium sp. g0005 produces a cell-bound glucoamylase (cga). the gene encoding cga has been sequenced. the deduced amino acid sequence begins with a putative 21-residue signal sequence for secretion of bacterial lipoproteins, which suggests that a putative cga precursor is modified and secreted like other bacterial lipoproteins in clostridium sp. g0005, and that the modified residue is important in the cell-bound form of mature cga. comparison of the amino acid sequence of the cga precursor w ... | 1992 | 1633799 |
| novel aerobic 2-aminobenzoate metabolism. nucleotide sequence of the plasmid carrying the gene for the flavoprotein 2-aminobenzoyl-coa monooxygenase/reductase in a denitrifying pseudomonas sp. | pseudomonas kb 740 degrades 2-aminobenzoate aerobically via a chimeric pathway which combines characteristics of anaerobic and aerobic aromatic metabolism. atypically, 2-aminobenzoyl-coa is an intermediate, and the activated aromatic acid is not only hydroxylated but also reduced to an alicyclic compound in a single step. the bacterial strain possesses a small plasmid, pkb 740, which carries all essential information of this new pathway. its total nucleotide sequence was determined. it consists ... | 1992 | 1633822 |
| phytochrome assembly. the structure and biological activity of 2(r),3(e)-phytochromobilin derived from phycobiliproteins. | the unicellular rhodophyte, porphyridium cruentum, and the filamentous cyanobacterium, calothrix sp. pcc 7601, contain phycobiliproteins that have covalently bound phycobilin chromophores. overnight incubation of solvent-extracted cells at 40 degrees c with methanol liberates free phycobilins that are derived from the protein-bound bilins by methanolytic cleavage of the thioether linkages between bilin and apoprotein. two of the free bilins were identified as 3(e)-phycocyanobilin and 3(e)-phycoe ... | 1992 | 1634523 |
| interaction of ap-1-, ap-2-, and sp1-like proteins with two distinct sites in the upstream regulatory region of the plasminogen activator inhibitor-1 gene mediates the phorbol 12-myristate 13-acetate response. | phorbol 12-myristate 13-acetate induces a 3- and 10-fold induction of chloramphenicol acetyltransferase (cat) activity in ht1080 and hela cells, respectively, following transient transfection of a 336-base pair plasminogen activator inhibitor-1 (pai-1) promoter fragment linked to a cat reporter gene. substitution mutations in the regions encompassing nucleotides -78 to -69 (tgggtggggc) or -61 to -54 (tgagttca), but not in the regions -155 to -149 (tgcctca) or -84 to -76 (agtgagtgg) reduced this ... | 1992 | 1634545 |
| interactions of surfactant protein d with bacterial lipopolysaccharides. surfactant protein d is an escherichia coli-binding protein in bronchoalveolar lavage. | surfactant protein d (sp-d) is a collagenous glycoprotein that is secreted into the pulmonary airspaces by alveolar type ii and nonciliated bronchiolar cells. sp-d exhibits ca(++)-dependent carbohydrate binding in vitro and is structurally related to the collagenous c-type lectins, including serum conglutinin, serum mannose-binding proteins, and surfactant protein a. preliminary studies showed calcium- and saccharide-dependent binding of fluorescein-conjugated or radioiodinated sp-d to a variety ... | 1992 | 1634623 |
| [effects of inner ear immune responses on auditory function in guinea pigs]. | following direct challenge with klh antigen primary or secondary (after systemic immunization) to the es (endolymphatic sac) in guinea pigs, ecog (electrocochleograms) were recorded from the round window induced by click and tone pips. the recordings were carried out on the 2nd, 7th and 21st days after local antigen challenge. there were no abnormal findings in the ecog of the primary antigen challenged animals. on the other hand, prolongation of latencies, decrease in amplitudes of aps (compoun ... | 1992 | 1634987 |
| biological characterization of trichinella isolates from various host species and geographical regions. | forty isolates of trichinella collected from 5 continents were compared for 7 biological characters: newborn larvae produced per female worm cultured in vitro at the seventh, eighth, and ninth day postinfection, host muscle nurse cell development time, reproductive capacity index in rats and chickens, and resistance of muscle larvae to freezing. the isolates also were compared by analyses of an environmental character of the location from which they were isolated: the isotherms for january and j ... | 1992 | 1635023 |
| taxonomic revision of the genus trichinella. | the analysis of genetic, biochemical, and biological data on about 300 trichinella isolates, reported in the literature, allows a taxonomic revision of this genus. we propose the recognition of 5 sibling species, trichinella spiralis (owen, 1835) sensu stricto; trichinella nativa britov and boev, 1972; trichinella pseudospiralis garkavi, 1972; trichinella nelsoni britov and boev, 1972 sensu stricto; and trichinella britovi n. sp., on the basis of biochemical and biological characteristics. trich ... | 1992 | 1635024 |
| protein kinase c activity during sphinganine potentiation of retinoic acid-induced differentiation in a human leukemia cell line (hl-60). | the differentiation of hl-60 promyelocytic cells toward mature granulocytic cells induced by retinoic acid (ra) was accompanied by a decrease in protein kinase c (pkc) activity. the enhancement of ra-induced differentiation and the potentiation of the decrease of pkc activity by sphinganine (sp) seemed to correlate with each other. kinetically, pkc activity during ra-induced differentiation without sp decreased to its lowest (75% of the control) after 48h; about 50% of the reduction was observed ... | 1992 | 1635422 |
| surfactant protein d: subcellular localization in nonciliated bronchiolar epithelial cells. | surfactant protein d (sp-d, cp4) is a collagenous surfactant-associated carbohydrate binding protein that was initially characterized as a biosynthetic product of type ii pneumocytes. immunoperoxidase studies of formaldehyde solution-fixed and paraffin-embedded rat lung demonstrated staining for sp-d in the cytoplasm of a subpopulation of bronchiolar epithelial cells as well as type ii cells. accordingly, immunogold-labeling techniques were used to further examine the cellular distribution and s ... | 1992 | 1636730 |
| microbial oxidation of oleic acid. | resting cells of saccharomyces cerevisiae (baker's yeast, type ii; sigma) were used to convert oleic acid into 10-hydroxyoctadecanoic acid with a 45% yield. nocardia aurantia (atcc 12674), nocardia sp. (nrrl 5646), and mycobacterium fortuitum (ui 53378) all converted oleic acid into 10-oxo-octadecanoic acid with 65, 55, and 80% yields, respectively. structures of all metabolites were suggested by 1h and 13c nuclear magnetic resonance and by infrared and mass spectrometry. structures of isomeric ... | 1992 | 1637152 |
| biosorption of dichlorodiphenyltrichloroethane and hexachlorobenzene in groundwater and its implications for facilitated transport. | the potential for enhanced mobility of hydrophobic pollutants by cotransport with bacteria in saturated soils was evaluated from measurements of biosorption of 14c-labeled hexachlorobenzene and dichlorodiphenyltrichloroethane (ddt) to five strains of soil and sewage bacteria. the sorption process could be described by a linear partition equation and appeared to be reversible, but desorption kinetics were slow and/or partly irreversible. the ddt partition coefficients varied with equilibration ti ... | 1992 | 1637158 |
| biodegradation of polycyclic aromatic hydrocarbons by new isolates of white rot fungi. | eight rapid poly r-478 dye-decolorizing isolates from the netherlands were screened in this study for the biodegradation of polycyclic aromatic hydrocarbons (pah) supplied at 10 mg liter(-1). several well-known ligninolytic culture collection strains, phanerochaete chrysosporium bkm-f-1767, trametes versicolor paprican 52, and bjerkandera adusta cbs 595.78 were tested in parallel. all of the strains significantly removed anthracene, and nine of the strains significantly removed benzo(a)pyrene be ... | 1992 | 1637159 |
| biodegradation of mixtures of substituted benzenes by pseudomonas sp. strain js150. | pseudomonas sp. strain js150 was isolated as a nonencapsulated variant of pseudomonas sp. strain js1 that contains the genes for the degradative pathways of a wide range of substituted aromatic compounds. pseudomonas sp. strain js150 grew on phenol, ethylbenzene, toluene, benzene, naphthalene, benzoate, p-hydroxybenzoate, salicylate, chlorobenzene, and several 1,4-dihalogenated benzenes. we designed experiments to determine the conditions required for induction of the individual pathways and to ... | 1992 | 1637161 |
| long-chain acyl-coa ester intermediates of beta-oxidation of mono- and di-carboxylic fatty acids by extracts of corynebacterium sp. strain 7e1c. | beta-oxidation of palmitate and tetradecanedioic acid was studied in cell-free extracts of the gram-positive bacterium corynebacterium sp. strain 7e1c, and the acyl-coa ester intermediates formed were analysed by h.p.l.c. beta-oxidation assays displayed a lag phase before a constant rate of nad+ reduction was obtained. the length of the lag phase was inversely proportional to the number of units of activity added to assays. this is a characteristic feature of a system of consecutive reactions pr ... | 1992 | 1637289 |
| instability of the protoplast membrane of facultative alkaliphilic bacillus sp. c-125 at alkaline ph values below the ph optimum for growth. | cell walls of facultative alkaliphilic bacillus sp. c-125 consist of three polymers (peptidoglycan, teichuronopeptide and teichuronic acid). protoplasts prepared from the strain with egg-white lysozyme regenerated cell walls at neutral ph, but not at ph above 8.5. the protoplasts were susceptible to lysis at alkaline ph. the protoplasts exposed to alkaline ph rapidly burst and lost ability to regenerate their cell walls. the alkali-instability was similar to that of protoplasts from neutrophilic ... | 1992 | 1637326 |
| purification and microsequencing of the intra-acrosomal protein sp-10. evidence that sp-10 heterogeneity results from endoproteolytic processes. | the human sperm antigen sp-10 has been shown to be a testis-specific, intra-acrosomal protein that is associated with the membranes and matrix of the acrosomal vesicle. sperm extracts, analyzed on western blots with a monoclonal antibody to sp-10, have shown heterogeneity of sp-10 peptides ranging from 17.5-34 kda. although the entire sp-10 amino acid sequence of 265 amino acids (28.3 kda) has been deduced from sequencing sp-10 cdnas, the nature of multiple sp-10 peptide bands is incompletely un ... | 1992 | 1637938 |
| neural changes in acute arthritis in monkeys. iv. time-course of amino acid release into the lumbar dorsal horn. | extracellular levels of amino acids were measured during the development of experimental arthritis in anesthetized monkeys. levels of glutamate, aspartate, glycine, serine, glutamine, taurine, cysteic acid and asparagine were each measured in consecutive 30 min samples before, during and for several hours after injection of kaolin and carrageenan into the articular capsule of one knee. samples were obtained via a microdialysis probe placed in the lumbar dorsal horn ipsilateral to the injected kn ... | 1992 | 1638274 |
| av3v lesion reduces the pressor, dipsogenic, and natriuretic responses to ventromedial hypothalamus activation. | in the present study, we investigated the effect of anteroventral third ventricle (av3v) lesion on pressor, tachycardic, dipsogenic, natriuretic, and kaliuretic responses induced by the injection of the cholinergic agonist carbachol into the ventromedial hypothalamic nucleus (vmh) of rats. male rats with sham or av3v lesion and a stainless steel cannula implanted into the vmh were used. carbachol (2 nmol) injected into the vmh of sham rats produced pressor (32 +/- 4 mmhg), tachycardic (83 +/- 14 ... | 1992 | 1638417 |
| insulin regulation of messenger ribonucleic acid for the surfactant-associated proteins in human fetal lung in vitro. | respiratory distress syndrome (rds) is primarily caused by an immaturity in the synthesis and secretion of surfactant by the fetal lung type ii cell. fetal hyperinsulinemia associated with maternal diabetes places the newborn at an increased risk of developing rds, and therefore, it has been hypothesized that insulin inhibits type ii cell differentiation. we have previously shown that insulin inhibits the accumulation of surfactant-associated protein a (sp-a), the major surfactant-associated pro ... | 1992 | 1639013 |
| lung surfactant protein a (sp-a) enhances serum-independent phagocytosis of bacteria by alveolar macrophages. | surfactant protein a (sp-a) is the main protein component of lung surfactant. we studied the involvement of sp-a in body defense, i.e., effect of sp-a on the phagocytosis of bacteria by alveolar macrophages. we show here that sp-a enhances the phagocytosis of some non-opsonized bacteria: escherichia coli growing logarithmically (e. coli/log), pseudomonas aeruginosa/log as well as from stationary phase (p. aeruginosa/stat) and staphylococcus aureus/log. furthermore, not only serum-independent pha ... | 1992 | 1639094 |
| detection of cellular immunity with the soluble antigen of the fungus sporothrix schenckii in the systemic form of the disease. | sporothrix schenckii is the etiologic agent of sporotrichosis, a mycosis of world-wide distribution more commonly occurring in tropical regions. the immunological mechanisms involved in the prevention and control of sporotrichosis are not fully understood but apparently include both the humoral and cellular responses. in the present investigation, cellular immunity was evaluated by in vivo and in vitro tests in mice infected with yeast-like forms of s. schenckii. the disease developed systemical ... | 1992 | 1640975 |
| structure of the pinworm (oxyurida: nematoda) guild in the hindgut of the american cockroach, periplaneta americana. | the pinworm (oxyurida: nematoda) guild in a laboratory colony of periplaneta americana was investigated to answer the following questions. (1) is guild structure stable with time? (2) is there evidence of interaction among the species? (3) is there evidence for niche diversification with respect to spatial distribution or developmental stage of host? four species were found: thelastoma periplaneticola, thelastoma bulhoesi, hammerschmidtiella diesingi and leidynema appendiculatum; females of the ... | 1992 | 1641249 |
| high levels of anti-campylobacter-flagellin iga antibodies in breast milk. | several studies have shown the beneficial role of immunity against enteric campylobacter sp. circulating antibodies against flagellin and possibly maternal antibodies seem to protect against intestinal colonization by campylobacter. milk from women in central africa (n = 120) and in france (n = 31) were investigated for the presence of anti-flagellin antibodies. results showed that most french and all african milk possessed anti-flagellin iga but not igg. the titres of anti-flagellin iga were el ... | 1992 | 1641516 |
| [antimicrobial sensitivity of haemophilus influenzae strains isolated from children]. | antibiotic susceptibility of 1308 haemophilus sp. strains isolated in children was evaluated over a 6-year period. based on their source and clinical presentation all strains were divided in three groups. the first group consisted of 1264 strains obtained by throat and nasal swab; the second of 30 mucosal strains isolated in children manifesting non-invasive infection and the third of 14 invasive strains, haemophilus influenzae(hi)tip b, isolated from blood, csf, pleural and synovial effusion. t ... | 1992 | 1641696 |
| [diagnosis of sarcocystosis in sheep using the indirect fluorescence test and elisa]. | the indirect fluorescent antibody test (ifat) was compared with the enzyme-linked immunosorbent assay (elisa) for the detection of specific antibodies to sarcocystis sp. a set of 275 ovine blood samples was examined by both reactions. cystozoites of sarcocystis gigantea were used as the corpuscular antigen for the ifat. for the diagnostics of sarcocystosis by the elisa technique used the sandwich test of the antibody titration with a soluble antigen which was also prepared from s. gigantea macro ... | 1992 | 1641936 |
| comparison of campylobacter carriage rates in diarrheic and healthy pet animals. | to explore the clinical significance of campylobacter infections for dogs and cats we compared intestinal carriage rates for campylobacter sp. between animals with gastroenteritis and healthy controls. we cultured fecal specimens of 405 diarrheic dogs and 203 cats as well as 71 asymptomatic dogs and 35 cats using a selective medium in addition to filtration on a non-selective blood agar plate. we identified 224 campylobacter isolates using conventional phenotypic tests and dna hybridization. the ... | 1992 | 1642073 |
| lipases from different sources vary widely in dependence of catalytic activity on water activity. | we have measured the rates of esterification in hexane catalysed by suspended immobilised lipases (triacylglycerol acylhydrolase, ec 3.1.1.3), with pre-equilibration to known thermodynamic water activity (a(w)). there were important differences between the enzymes from five different microbes in their retention of activity at low a(w). that from rhizomucor miehei showed over 40% maximal activity at an a(w) of 0.12, and that from rhizopus niveus was also fairly active at low a(w). lipases from ot ... | 1992 | 1643087 |
| cdna sequence and alternative mrna splicing of surfactant-associated protein c (sp-c) in rabbit lung. | an 784 base pair (bp) copy dna (cdna) for the low molecular weight hydrophobic surfactant-associated protein c (sp-c) has been isolated from a lambda gt11 cdna library constructed from fetal rabbit lung mrna. the cdna, which coded for a 193 amino-acid proprotein with 6 bp 5' and 193 bp 3' untranslated segments, possesses considerable nucleic acid and predicted amino-acid homology with previously reported sp-c cdnas. the predicted amino-acid sequence of the 35 amino-acid mature polypeptide shares ... | 1992 | 1643107 |
| report on an outbreak of trichinellosis in central italy. | at the end of 1988 an outbreak of trichinellosis involving 48 persons occurred in a small town in central italy, near the appennine mountains. the epidemic was caused by the ingestion of sausages recently made with wild boar meat. the people affected were mostly relatives and friends of hunters. trichinella larvae were found in boar meat sausages and in cats fed with raw leftovers and proved to be trichinella sp. 3 at biochemical typing. this species of parasite is the most frequently isolated s ... | 1992 | 1644150 |
| a new type of mitogenic factor produced by streptococcus pyogenes. | a new type of mitogenic factor (protein) was purified from the culture supernatant of a strain of streptococcus pyogenes by sp-sephadex c-25 column chromatography, preparative isoelectric focusing and reversed-phase high-performance liquid chromatography. the purified factor, showing marked mitogenic activity in rabbit peripheral blood lymphocytes, gave a single-band staining for protein on sds-page. the molecular weight of the purified mitogenic factor was determined to be 25,370, which was dif ... | 1992 | 1644200 |
| a domestic duck as a new host for blastocystis sp. | | 1992 | 1644350 |
| ewingana (doreyana) baekelandae, sp. n., a parasite of molossid bats in spain (acari: myobiidae). | ewingana (doreyana) baekelandae sp. n. (acari: myobiidae) is described on the basis of several adults and immatures collected on tadarida (t.) teniotis from the province of teruel, spain. the species is a palearctic representative of the evolutive line nanula fain and digitata fain. differences within these species are observed in the female genito-anal area and in the shape and size of leg i, both in adults and in immatures. | 1992 | 1644352 |
| aponomma orlovi sp. n.--a new species of ixodid ticks (acarina, ixodidae) from vietnam. | | 1992 | 1644356 |
| comparative morphology of eggs of the haplorchiinae (trematoda: heterophyidae) and some other medically important heterophyid and opisthorchiid flukes. | the egg morphology of the following medically important small flukes from southeast asia and far east were studied: opisthorchis viverrini, clonorchis sinensis (opisthorchiidae), haplorchis taichui, h. pumilio, h. yokogawai, stellantchasmus falcatus and metagonimus sp. (heterophydiae). this study revealed a great intraspecific variability and interspecific similarity in size and shape of eggs. the eggs shape does not seem to be suitable for species identification. on the other hand, biometrical ... | 1992 | 1644359 |
| helminth eggs in the sludge from three sewage treatment plants in czechoslovakia. | developmental stages of 8 species of parasitic helminths were found during examination of sludge from 3 sewage treatment plants in central bohemia, czechoslovakia. the eggs were comprised from ascaris sp., toxocara sp., toxascaris leonina, parascaris equorum, enterobius vermicularis, trichuris sp., capillaria sp. and hymenolepis spp. the quantity of these organisms in 100 g of dried sludge ranged between 24-105 eggs of ascaris sp., 12-47 eggs of toxocara sp., 0-8 eggs of toxascaris leonina, 0-1 ... | 1992 | 1644362 |
| the cpce and cpcf genes of synechococcus sp. pcc 7002. construction and phenotypic characterization of interposon mutants. | the 3' region of the cpc operon of synechococcus sp. pcc 7002 has been sequenced, transcriptionally characterized, and analyzed by interposon mutagenesis. the cpc operon contains six genes, 5' cpcb-cpca-cpcc-cpcd-cpce-cpcf 3', and gives rise to at least eight (more likely ten) discrete mrna transcripts. the steady-state levels of transcripts for the cpce and cpcf genes are very low and are estimated to represent only about 1-2% of the total transcripts arising from the cpc locus. the cpce gene p ... | 1992 | 1644801 |
| characterization of phycocyanin produced by cpce and cpcf mutants and identification of an intergenic suppressor of the defect in bilin attachment. | mutants of the cyanobacterium synechococcus sp. pcc 7002 constructed by the insertional inactivation of either the cpce or cpcf gene produce low levels of spectroscopically detectable phycocyanin. the majority of the phycocyanin produced in these strains appears to lack the alpha subunit phycocyanobilin (pcb) chromophore (zhou, j., gasparich, g. e., stirewalt, v. l., de lorimier, r., and bryant, d. a. (1992) j. biol. chem. 267, 16138-16145). purification of the phycocyanin produced in the mutant ... | 1992 | 1644802 |
| the activation of camp-dependent protein kinase is directly linked to the regulation of osteoblast proliferation (umr-106) by parathyroid hormone. | in order to characterize the direct involvement of camp in the change of osteoblast proliferation by parathyroid hormone (pth), we employed the diastereoisomers of adenosine 3',5'-cyclic phosphorothioate, sp-camps and rp-camps, which have been recently shown to act directly as agonist and antagonist, respectively in the activation of camp-dependent protein kinase (pka). dibutyryl camp (dbcamp) and cholera toxin as well as human(h) pth-(1-34) significantly inhibited [3h]thymidine incorporation (t ... | 1991 | 1645960 |
| spectral properties of achromobacter xylosoxidans cytochromes c' and their no complexes. | cytochromes c' have been isolated from six strains of achromobacter xylosoxidans: ncib 11015 (formerly alcaligenes sp. ncib 11015), gifu 543, 1048, 1051, 1055 and 1764. they are dimeric proteins with more positive redox potentials than those of cytochromes c' from phototrophic bacteria at neutral ph. the electronic absorption, epr and mcd spectra on no-ferrous cytochromes c' at physiological ph showed that the major part of the heme-iron of nitrosylheme was penta-coordinated. the epr spectral re ... | 1991 | 1646026 |
| lung transplantation: the pathologic diagnosis of pulmonary complications. | the reliability of bronchoscopy with transbronchial biopsies for the diagnosis of acute graft rejection has recently been questioned. we present our experience with 59 transbronchial and bronchial biopsies and two open-lung biopsies from 12 patients that underwent lung transplantation. the diagnosis of acute rejection was established in 14 biopsies based on the absence of infection and presence of one or more of the following features: perivascular lymphoid infiltrates, usually associated with e ... | 1991 | 1646451 |
| transposon vectors for stable chromosomal integration of cloned genes in rhizosphere bacteria. | a series of tn5-based transposon-cloning vectors, in which many unique restriction sites lie within the transposon, have been constructed. these transposon vectors can be delivered, by conjugation, using a delivery vehicle containing a pbr322 replicon and the mobilization genes of plasmid rk2. in pseudomonas sp., this delivery vehicle acts as a suicide plasmid, permitting transposition to the chromosome to be detected. to facilitate cloning into the transposon vector, the delivery vehicle has be ... | 1991 | 1647354 |
| alternative mobile phases for enhanced chromatographic selectivity and increased sensitivity in peptide separations. | the standard method for separating peptide mixtures is reversed-phase high-performance liquid chromatography with gradients of increasing concentrations of acetonitrile in the presence of trifluoroacetic acid. with modern instruments and columns, complex peptide mixtures can be separated, and low picomole amounts can be collected in tens of microliters. difficult separations are addressed by modifying the gradient slope or organic eluant composition. further improvements in resolution are often ... | 1991 | 1647805 |
| mutations of the alpha-galactosidase signal peptide which greatly enhance secretion of heterologous proteins by yeast. | the saccharomyces carlsbergensis mel1 gene encodes alpha-galactosidase (melibiase; mel1) which is readily secreted by yeast cells into the culture medium. to evaluate the utility of the mel1 signal peptide (sp) for the secretion of heterologous proteins by saccharomyces cerevisiae, an expression vector was constructed which contains the mel1 promoter and mel1 sp coding sequence (mel1sp). the coding sequences for echistatin (echis) and human plasminogen activator inhibitor type 1 (pai-1) were ins ... | 1991 | 1648007 |
| [experimental research on the effect of biologically active substances on the susceptibility of mosquitoes to the causative agent of malaria. 3. algae, fertilizers]. | on a model pair aedes aegypti--plasmodium gallinaceum in has been shown that changes in the conditions of larvae development caused by the addition into the water medium of the live culture of synochocystis sp. cyanobacteria or green seaweeds chlorella vulgaris, acetone extracts from the live culture precipitate or chlorella powder, as well as nitrogen-containing fertilizer--ammonium chloride did not lower the sensitivity of the imago flying to malaria parasites. the results of the experiments a ... | 1991 | 1648659 |
| surfactant protein b: disulfide bridges, structural properties, and kringle similarities. | the disulfide bridges in porcine hydrophobic surfactant protein b (sp-b) were determined. results show that three intrachain bridges link half-cystine residues 8 and 77, 11 and 71, and 35 and 46, respectively. this gives sp-b an appearance of three loops, a central big loop surrounded by two smaller ones. in the major form of sp-b, the remaining half-cystine, cys-48, is probably interchain-linked to its counterpart in another molecule, compatible with the existence of dimeric molecules. a minor ... | 1991 | 1648964 |
| secondary metabolites by chemical screening. part 19. sm 196 a and b, novel biologically active angucyclinones from streptomyces sp. | | 1991 | 1649152 |
| prophylaxis against organophosphate poisoning by an enzyme hydrolysing organophosphorus compounds in mice. | parathion hydrolase purified from pseudomonas sp. was injected i.v. into mice to demonstrate the feasibility of using organophosphorus acid anhydride (opa) hydrolases as pretreatment against organophosphates (op) poisoning. results show that exogenous administration of as low as 7 to 26 micrograms of parathion hydrolase conferred protection against challenge with multiple median lethal doses (ld50) of diethyl p-nitrophenyl phosphate (paraoxon; 3.8-7.3 x ld50) and diethylfluorophosphate (defp; 2. ... | 1991 | 1649941 |
| an extremely thermostable xylanase from the thermophilic eubacterium thermotoga. | endo-1,4-beta-xylanase (ec 3.2.1.8) was isolated from the culture supernatant of thermotoga sp. strain fjss3-b.1, an extremely thermophilic anaerobic eubacterium which grows optimally at 80 degrees c. activity was purified 165-fold by anion-exchange and hydroxyapatite chromatography. the enzyme has an mr of 31,000 as determined by sds/page and 35,000 by analytical gel filtration. the optima for activity and stability for purified xylanase were between ph 5.0 and 5.5. at ph 5.5, which is the opti ... | 1991 | 1650183 |
| fragmentation of heparin by enzymes from newly isolated microorganisms. | in a screening program a number of new heparin (cas 9005-49-6) degrading microorganisms from soil and water samples from europe, asia and australia were isolated. the new strains were identified as cytophaga sp. but are distinct from the known heparin degrading organism cytophaga heparina in various aspects of cell morphology, sugar metabolism and heparin utilization. analysis of heparin degradation by crude extracts from cytophaga heparina-, tm5- and sin1-cells shows that the products obtained ... | 1991 | 1650230 |
| [a two-year prospective survey on nosocomial infections]. | a two-year randomized prospective survey on nosocomial infections in hospitalized patients of hua shan hospital from 1985 to 1987 was carried out. altogether 1,826 patients were enrolled in the study, the incidence of nosocomial infection being 13.1%. the incidence in dermatology ward was the highest (19.8%), then in order of frequency; medical ward 16.5%, surgical ward 14.8%, ward of neurology 13.7%, ward of neurosurgery 12.7% etc. regarding the location of infection, lower respiratory tract in ... | 1991 | 1650640 |
| sequence of the gene (phea) encoding phenol monooxygenase from pseudomonas sp. est1001: expression in escherichia coli and pseudomonas putida. | the plasmid pest1412 contains the genes, phea and pheb, encoding phenol monooxygenase (pmo) and catechol 1,2-dioxygenase (c12]), respectively. thse were originally cloned from the plasmid dna of pseudomonas sp. est1001 [kivisaar et al., plasmid 24 (1990) 25-36]. although phea and pheb are cotranscribed using the promoter sequences derived from tn4652 and the level of expression of c120 activities from pest1412 was equal both in escherichia coli and in pseudomonas putida, the level of pmo activit ... | 1991 | 1650730 |
| psad is required for the stable binding of psac to the photosystem i core protein of synechococcus sp. pcc 6301. | the psac gene product from synechococcus sp. pcc 7002 and the psad gene product from nostoc sp. pcc 8009 were synthesized in escherichia coli and purified to homogeneity. incubation of the psac apoprotein with the synechoccus sp. pcc 6301 photosystem i core protein in the presence of fecl3, na2s, and beta-mercaptoethanol resulted in a time-dependent transition in the flash-induced absorption change from a 1.2-ms, p700+ fx- back-reaction to a long-lived, p700+ [fa/fb]- back-reaction. esr studies ... | 1991 | 1651109 |
| surfactant protein a is opsonin in phagocytosis of herpes simplex virus type 1 by rat alveolar macrophages. | in the present study we used flow cytometry to investigate the phagocytosis of fluorescein isothiocyanate-labeled herpes simplex virus type 1 (fitc-hsv-1) by rat alveolar macrophages and the effects of surfactant protein a (sp-a) on this process. the phagocytosis of fitc-hsv-1 by alveolar macrophages, which was studied as a model for virus phagocytosis in general, was strongly enhanced in the presence of sp-a. the sp-a-mediated phagocytosis was time and concentration dependent, reaching a maxima ... | 1991 | 1651668 |
| developmental and hormonal regulation of surfactant protein a (sp-a) gene expression in fetal lung. | pulmonary surfactant is a developmentally-regulated lipoprotein synthesized and secreted by the type ii cells of the pulmonary alveolus where surfactant glycerophospholipids and proteins act to reduce surface tension at the alveolar air-liquid interface. surfactant protein a (sp-a), the major surfactant-associated protein, appears to serve an important role in surfactant function and reutilization by type ii cells. sp-a synthesis and gene expression are initiated in fetal lung tissue in concert ... | 1991 | 1651967 |
| proteins of the nuclear factor-1 family act as an activator of the late promoter in human polyomavirus bk in vitro. | the cis-acting elements for the early and late promoters, as well as the enhancer in the prototype strains of human polyomavirus bk (bkv) are located within a 500 bp intergenic region. we previously studied the specificity of protein binding in this region in vitro and showed that the interaction of proteins of the nuclear factor-1 (nf-1) family is crucial for early promoter activity. we have now extended our study to the bkv late promoter. we show that the late promoter activity in hela cell ex ... | 1991 | 1651986 |
| fine mapping and characterization of the syn 6 locus in the herpes simplex virus type 1 genome. | the syncytial mutant of herpes simplex virus type 1 (hsv-1), hsv-1(13) s11, which carries three distinct syncytial mutations, syn 1, syn 5 and syn 6, was described previously. syn 1 maps to the bamhi l fragment, map units (m.u.) 0.707 to 0.745; syn 5 is located within the bamhi q fragment, m.u. 0.296 to 0.317; syn 6 lies in the junction fragment bamhi sp, m.u. 0.81 to 0.85. although syn 1 of hsv-1(13) s11 seems to be homologous to that of hsv-1(mp) and other syncytial mutants, and syn 5 has been ... | 1991 | 1651990 |
| human chorionic gonadotropin in esophageal carcinomas. an immunohistochemical study. | we have examined immunohistochemically the presence of human chorionic gonadotrophin (hcg) in 29 esophageal carcinomas: 24 squamous cell carcinomas, 2 adenocarcinomas, 2 adenoid cystic carcinomas and 1 adenosquamous carcinoma. in hcg-positive tumors, the presence of human placental lactogen (hpl) and pregnancy-specific beta-1 glycoprotein (sp-1) was also assessed. hcg immunoreactive cells were found in 5 squamous cell carcinomas (21%) and in none of 5 non-squamous cell tumors. the hcg positive c ... | 1991 | 1652131 |
| sensitive immunometric assays for secretory peroxidase and myeloperoxidase in human saliva. | we have developed specific immunoassays for secretory peroxidase (sp) and for myeloperoxidase (mp) (polymorphonuclear leukocyte-derived peroxidase) in human saliva. antibodies against sp and mp were produced using bovine milk lactoperoxidase (lp) and human mp as the immunogens, respectively. the methods developed are non-isotopic immunometric assays using biotinylated antibodies and avidin-enzyme conjugate. the detection limit was 0.1 ng/ml and the performance time less than 3 h for both assays. ... | 1991 | 1652610 |
| [therapeutic efficacy of imipenem/cilastatin sodium on respiratory tract infections in lung cancer patients]. | imipenem/cilastatin sodium (ipm/cs) was used to treat respiratory tract infections (rti) in 54 patients with lung cancer. out of the 54 patients studied, 53 were evaluable for the utility of ipm/cs; 42 had pneumonia, 9 had obstructive pneumonia, 1 had a lung abscess and 1 had acute bronchitis. the efficacy rate was 71.7%. seventeen causative organisms were isolated from 14 patients. they included staphylococcus aureus 5 strains, staphylococcus epidermidis 4 strains, staphylococcus sp. 2 strains, ... | 1991 | 1652653 |
| polypeptide composition of the photosystem i complex and the photosystem i core protein from synechococcus sp. pcc 6301. | the polypeptide composition of the photosystem i complex from synechococcus sp. pcc 6301 was determined by sodium-dodecyl sulfate polyacrylamide gel electrophoresis and n-terminal amino acid sequencing. the psaa, psab, psac, psad, psae, psaf, psak and psal proteins, as well as three polypeptides with apparent masses less than 8 kda and small amounts of the 12.6 kda glnb (pii) protein, wee present in the photosystem i complex. no proteins homologous to the psag and psah subunits of eukaryotic pho ... | 1991 | 1653017 |
| characterization of insertion sequence is892 and related elements from the cyanobacterium anabaena sp. strain pcc 7120. | is892, one of the several insertion sequence (is) elements discovered in anabaena sp. strain pcc 7120 (y. cai and c. p. wolk, j. bacteriol. 172:3138-3145, 1990), is 1,675 bp with 24-bp near-perfect inverted terminal repeats and has two open reading frames (orfs) that could code for proteins of 233 and 137 amino acids. upon insertion into target sites, this is generates an 8-bp directly repeated target duplication. a 32-bp sequence in the region between orf1 and orf2 is similar to the sequence of ... | 1991 | 1653218 |
| characterization of the is895 family of insertion sequences from the cyanobacterium anabaena sp. strain pcc 7120. | a family of repetitive elements from the cyanobacterium anabaena sp. strain pcc 7120 was identified through the proximity of one element to the psbai gene. four members of this seven-member family were isolated and shown to have structures characteristic of bacterial insertion sequences. each element is approximately 1,200 bp in length, is delimited by a 30-bp inverted repeat, and contains two open reading frames in tandem on the same dna strand. the four copies differ from each other by small i ... | 1991 | 1653219 |
| identifying the molecular switches that determine whether (rp)-camps functions as an antagonist or an agonist in the activation of camp-dependent protein kinase i. | previous investigations revealed that under physiological conditions in the presence of mgatp the phosphorothioate analogue of camp, (rp)-camps, is a competitive inhibitor and antagonist for camp for camp-dependent protein kinases i and ii [dewit et al., (1984) eur. j. biochem. 142, 255-260]. for the type i holoenzyme, the antagonist properties of (rp)-camps are shown here to be absolutely dependent on mgatp. in the absence of mgatp, (rp)-camps serves as a weak agonist with a ka of 7.9 microm. t ... | 1991 | 1653606 |
| posttranscriptional regulation of surfactant protein-a messenger rna in human fetal lung in vitro by glucocorticoids. | surfactant protein-a (sp-a), the major pulmonary surfactant-associated protein, is a developmentally and hormonally regulated sialoglycoprotein of about 35,000 mol wt. in previous studies we observed that dexamethasone has dose-dependent biphasic effects on the levels of sp-a and its mrna in human fetal lung in vitro. at concentrations of 10(-10)-10(-9) m, dexamethasone increased the levels of sp-a and its mrna over those of control tissues, whereas at concentrations greater than or equal to 10( ... | 1991 | 1653895 |
| archaerhodopsin-2, from halobacterium sp. aus-2 further reveals essential amino acid residues for light-driven proton pumps. | we have isolated a retinal protein which differs from bacteriorhodopsin and archaerhodopsin and pumps out as many protons in the light as those proton pumps. we tentatively named it archaerhodopsin-2. we have cloned and sequenced the gene that encodes archaerhodopsin-2. the gene consists of 780-bp nucleotides for 259 amino acids with a molecular mass of 27,937 da. the amino acid sequence of archaerhodopsin-2 is 56% identical to bacteriorhodopsin and 88% to archaerhodopsin, with a few gaps of a f ... | 1991 | 1654776 |
| inhibition of fetal rat pancreatic beta-cell replication by interleukin-1 beta in vitro is not mediated through pertussis toxin-sensitive g-proteins, a decrease in cyclic amp, or protease activation. | it has been proposed that the cytokine interleukin-1 beta (il-1 beta), secreted by islet-infiltrating macrophages, may be involved in the pathogenesis of insulin-dependent diabetes mellitus by participation in beta-cell destruction. addition of il-1 beta to isolated pancreatic islets in vitro results in cytotoxic effects on beta-cell function, but there is little information on the intracellular events that convey the actions of the cytokine. in the present study, fetal rat pancreatic islets con ... | 1991 | 1655527 |
| chromosomal mapping of two genetic loci associated with blood-pressure regulation in hereditary hypertensive rats. | the spontaneously hypertensive rat and the stroke-prone spontaneously hypertensive rat are useful models for human hypertension. in these strains hypertension is a polygenic trait, in which both autosomal and sex-linked genes can influence blood pressure. linkage studies in crosses between the stroke-prone spontaneously hypertensive rat and the normotensive control strain wistar-kyoto have led to the localization of two genes, bp/sp-1 and bp/sp-2, that contribute significantly to blood pressure ... | 1991 | 1656270 |
| chlorobenzoate catabolic transposon tn5271 is a composite class i element with flanking class ii insertion sequences. | the structure of a transposon specifying the biodegradation of chlorobenzoate contaminants is described. tn5271 is a 17-kilobase (kb) transposon that resides in the plasmid or chromosome of alcaligenes sp. strain br60 and allows this organism to grow on 3- and 4-chlorobenzoate. the transposon is flanked by a directly repeated sequence of 3201 base pairs (bp), which in turn is flanked by 110-bp inverted repeats. the 3.2-kb repeated sequence, designated is1071, exists in multiple copies in the gen ... | 1991 | 1656436 |
| neurovirulence of six different murine coronavirus jhmv variants for rats. | six variant viruses of the jhmv strain of murine coronavirus with large (cl-2, cnsv, dl and ds) or small (sp-4 and jhm-x) s proteins were compared in terms of their relative neurovirulence in weanling lewis rats. inoculation of various doses of the variants revealed that the cl-2 and cnsv were highly virulent and dl and ds were low-virulent, while sp-4 and jhm-x were avirulent. pathological examination of rats infected with variants cl-2, dl and sp-4 showed that the cl-2 and dl induced severe an ... | 1991 | 1656623 |