Publications

TitleAbstractYear
Filter
PMID
Filter
spectroscopic characterization of two peptides derived from the stem of rabies virus glycoprotein.rabies virus glycoprotein (g) is a trimeric type i transmembrane glycoprotein that mediates both receptor recognition and low ph-induced membrane fusion. electron microscopy has indicated that the ectodomain of protein g is made of a globular head and a stem. in order to characterize the putative stem region at the molecular level, we designed two peptides, p(s) and p(l), which were produced as gst fusion proteins in bacteria. peptide p(s) extends from amino acid (aa) 374 to aa 428 whereas pepti ...200312782363
inactivated suckling mouse brain rabies vaccine provides short-term immunity in capuchin monkeys (cebus apella).eight capuchin monkeys (cebus apella) were vaccinated against rabies with an inactivated suckling mouse brain vaccine (smbv). three 1-ml doses of 2% brain tissue suspension were given by i.m. injection at 0, 30, and 60 days. blood samples were collected at 0, 30, 60, 90, 150, 210, 240, 300, and 365 days and were tested by simplified fluorescence inhibition to titer-neutralizing antibodies. all of the animals developed neutralizing antibodies with titers >0.5 iu/ml after vaccination, but the immu ...200112790394
exposure of hooded capuchin monkeys (cebus apella cay) to a rabid bat at a zoological park.on 27 may 1999, a big brown bat (eptesicus fuscus) was discovered on an island exhibit at the denver zoo that contained a troop of 15 hooded capuchin monkeys (cebus apella cay). the monkeys were attacking the bat when it was discovered. the bat was collected and humanely euthanatized without direct handling and submitted to the colorado department of public health and environment virology laboratory for rabies evaluation. the monkeys had not been vaccinated against rabies virus. the next day, th ...200112790408
antibody response to rabies vaccination in captive and free-ranging wolves (canis lupus).fourteen captive and five free-ranging minnesota gray wolves (canis lupus) were tested for the presence of rabies virus neutralizing antibodies (rvna) after vaccination with an inactivated canine rabies vaccine. blood was collected from all wolves prior to vaccination and at 1 mo postvaccination (pv) and from all captive and three wild wolves at 3 mo pv. in addition, one free-ranging wolf was sampled at 4 mo pv, and two free-ranging wolves were sampled at 6 mo pv. all wolves were seronegative pr ...200112790409
function and glycosylation of plant-derived antiviral monoclonal antibody.plant genetic engineering led to the production of plant-derived mab (mabp), which provides a safe and economically feasible alternative to the current methods of antibody production in animal systems. in this study, the heavy and light chains of human anti-rabies mab were expressed and assembled in planta under the control of two strong constitutive promoters. an alfalfa mosaic virus untranslated leader sequence and lys-asp-glu-leu (kdel) endoplasmic reticulum retention signal were linked at th ...200312799460
common host genes are activated in mouse brain by japanese encephalitis and rabies viruses.this study identified nine genes whose expression is upregulated in the central nervous system (cns) of mice during japanese encephalitis virus (jev) infection. these include: cathepsin s, oligoadenylate synthetase (oas), garg49/irg2, lymphocyte antigen-6a (ly-6a), macrophage activation gene-2 (mpa2), early growth response gene1 (egr1), pyrimidine 5'-nucleotidase (p5n), apolipoprotein d (apod) and stat1. activation of all nine genes during jev infection was confirmed by northern blot analysis. j ...200312810866
development of a cocktail of recombinant-expressed human rabies virus-neutralizing monoclonal antibodies for postexposure prophylaxis of rabies.to provide a cost-effective and safe replacement for human rabies immunoglobulin (hrig), we used dna recombinant technology to express 3 human rabies virus-neutralizing human monoclonal antibodies (humabs) in a rhabdovirus vector (rhv). infection of either baby hamster kidney cells or cho cells, with the resulting rhv-humab recombinant viruses, yielded high-level production (< or =40 micro g/ml/48 h) of rhv recombinant-expressed humabs (rhumabs) that differ in both isotype and epitope-recognitio ...200312825170
seroconversion in captive african wild dogs (lycaon pictus) following administration of a chicken head bait/sag-2 oral rabies vaccine combination.this study determined the proportion of captive juvenile and adult african wild dogs (lycaon pictus) that developed protective titres of rabies neutralising antibodies following ingestion of a chicken head bait/sag-2 oral rabies vaccine combination. a single chicken head containing 1.8 ml of sag-2 vaccine (10(8.0) tcid50/ml) in a plastic blister was fed to each of eight adult and three juvenile wild dogs. bait ingestion resulted in a significant rise in serum neutralising antibody titres. overal ...200312825684
further characterization of the rabies virus glycoproteins produced by virus-infected and g cdna-transfected cells using a monoclonal antibody, #1-30-44, which recognizes an acid-sensitive epitope.expression of rabies virus glycoprotein (g) by g cdna-transfected mammalian cells resulted in the production of only a fusion-negative form. low ph-dependent fusion activity, however, was seen when the expression was done under control of the t7 promoter with the help of recombinant vaccinia virus (rvv-t7) that provided t7 rna polymerase. fusion-inactive g proteins were transported to the cell surface as being detected by a conformational epitope-specific monoclonal antibody (mab; #1-46-12). the ...200312825895
in situ reverse transcription-polymerase chain reaction: a novel technique for detection of rabies virus rna in murine neuroblastoma cells. 200312828347
development and evaluation of a latex agglutination test for rabies antibodies.the presently recommended tests for estimation of rabies neutralising antibodies like rapid fluorescent focus inhibition test (rffit) and mouse neutralisation test (mnt) are laborious, time consuming and not cost-effective for routine use. simple, rapid and economical tests need to be developed for routine use.200312829034
rabies update for travel medicine advisors.rabies is a neglected disease in many developing countries. it is preventable, and the tools to prevent it are known. there is urgent need for more funding, for study of innovative dog population-control measures, and for sustainable canine immunization. safe and effective tissue-culture rabies vaccines and human and equine rabies immunoglobulins (hrig and erig) are not readily available in many regions where rabies is endemic. this and the continuing presence and spread of rabies have increased ...200312830414
human rabies: a disease of complex neuropathogenetic mechanisms and diagnostic challenges.rabies is inevitably fatal and presents a horrifying clinical picture. human rabies can manifest in either encephalitic (furious) or paralytic (dumb) forms. the brainstem is preferentially involved in both clinical forms, though there are no clinical signs of brainstem dysfunction. differences in tropism at the inoculation site or the cns, in the route of spread, or in the triggering of immune cascades in the brainstem may account for clinical variation. rabies still poses diagnostic problems, p ...200212849514
evaluation of lumpy skin disease virus, a capripoxvirus, as a replication-deficient vaccine vector.lumpy skin disease virus (lsdv), a capripoxvirus with a host range limited to ruminants, was evaluated as a replication-deficient vaccine vector for use in non-ruminant hosts. by using the rabies virus glycoprotein (rg) as a model antigen, it was demonstrated that recombinant lsdv encoding the rabies glycoprotein (rlsdv-rg) was able to express rg in both permissive (ruminant) and non-permissive (non-ruminant) cells. the recombinant lsdv, however, replicated to maturity only in permissive but not ...200312867628
viral meningitis and encephalitis: traditional and emerging viral agents.in the united states, the annual number of central nervous system (cns) infections that occur as a result of viral agents far exceeds that of infections caused by bacteria, yeast, molds, and protozoa combined. the recent incursion of west nile virus (wnv) into north america has led to a dramatic change in the incidence and epidemiology of summer-associated viral cns disease. as a result of increased testing for wnv, lesser known viral causes of cns infection have been identified. even the epidem ...200312881794
cross-reactive antigenicity of nucleoproteins of lyssaviruses recognized by a monospecific antirabies virus nucleoprotein antiserum on paraffin sections of formalin-fixed tissues.diagnosis of rabies is routinely confirmed by detection of rabies virus antigens in acetone-fixed frozen brain tissues or imprint smears using an immunofluorescence method with commercial antirabies virus antibodies. since recent molecular analyses disclosed wide heterogeneity in the genome sequences of rabies virus strains and related lyssaviruses, it is necessary to confirm the presence of common epitopes in these lyssaviruses. in this study we confirmed the presence of cross-reactive antigens ...200312895231
new approaches to the prevention and eradication of rabies.despite significant progress in improving the pre- and postexposure prophylaxis of human rabies, the development of better and more cost-effective vaccines and antiviral therapeutics remains a major goal for the treatment of human rabies, the control of animal rabies and particularly for the eradication of rabies virus reservoirs in terrestrial wildlife. in this review, we discuss the structural requirements for an effective rabies vaccine, as well as new strategies currently in use for the deve ...200312903805
[antibodies to the rabies virus in humans working in a veterinary hospital environment at the universidade federal de mato grosso. cuiabá, mt, brazil].a characterization of the serological profile of 102 workers in constant activities in a veterinary hospital environment at the universidade federal de mato grosso was made through epidemiological data and serological tests to the rabies virus from november 1999 to november 2000. of these, 27 had been vaccinated in a pre-exposure scheme and 75 had not received any kind of antirabies immunization. the results of the study classified the people into four groups (g1, g2, g3 and g4). in g1, 19 (18.6 ...200312908036
rabies among infrequently reported mammalian carnivores in the united states, 1960-2000.most cases of rabies reported annually in the united states occur among three groups of carnivores--raccoons (procyon lotor), skunks (mephitis, spilogale, and putorius), foxes (vulpes, urocyon, and alopex)--and among bats (numerous species). however, between 1960 and 2000, a total of 2,851 cases of rabies in 17 other carnivore taxa were reported to the centers for disease control and prevention, atlanta, georgia (usa), from 49 states, the district of columbia, and puerto rico. three species of t ...200312910751
rabies emergence among foxes in turkey.sixteen rabies isolates recently collected from mainland turkey and two isolates held within a british archive were used to form a representative cohort from a range of vectors, and were analyzed to identify potential causes for an increase of rabies within the fox (vulpes vulpes) population in turkey. each isolate was characterized by sequence analysis of the nucleoprotein gene and compared phylogenetically to the cohort, to isolates from neighboring countries and to isolates from continental e ...200312910752
the persistence of rabies virus antibodies in the sera of fox cubs.the persistence of maternal antibodies transfer from rabies-immune vixens to their fox cubs was studied. eight vixens (vulpes vulpes) were vaccinated 1 month before pregnancy with lysvulpen vaccine for oral vaccination of foxes. twenty-one were foxes born at the first half of april. the geometrical mean titre of rabies neutralizing antibodies of fox cubs sampled in may was 1.31 iu/ml and has dropped successively to 0.54 iu/ml in june samples and to 0.18 iu/ml in july samples. it has been proven ...200312916696
canine rabies dna vaccination: a single-dose intradermal injection into ear pinnae elicits elevated and persistent levels of neutralizing antibody.rabid dog exposures cause >99% of human rabies deaths world-wide. in developing countries, where dogs are the viral reservoir, the 30-50% vaccination coverage of dog populations is insufficient to break the disease transmission cycle. in addition, many vaccines currently used in developing countries fail to maintain detectable levels of neutralizing antibody. the poor vaccination coverage with inadequate vaccines, in addition to the difficulty in locating dogs for booster vaccinations, suggest t ...200312922136
study of lyssaviruses of bat origin as a source of rabies for other animal species in the state of rio de janeiro, brazil.an epidemic-geographic rabies study was carried out in which 72 animal and human brain samples were analyzed for lyssaviruses by a direct immunofluorescent technique (dift) and a reverse transcriptase-polymerase chain reaction (rt-pcr) assay. fifty-two samples were also tested by a mouse inoculation test. lyssavirus rna was detected in 60 of 72 samples. five dift-negative bat samples tested by a nested pcr assay showed evidence of the presence of rabies virus rna. sequencing of amplified rabies ...200312932103
in vitro and in vivo gene delivery by recombinant baculoviruses.although recombinant baculovirus vectors can be an efficient tool for gene transfer into mammalian cells in vitro, gene transduction in vivo has been hampered by the inactivation of baculoviruses by serum complement. recombinant baculoviruses possessing excess envelope protein gp64 or other viral envelope proteins on the virion surface deliver foreign genes into a variety of mammalian cell lines more efficiently than the unmodified baculovirus. in this study, we examined the efficiency of gene t ...200312941888
[the antiviral activity of the peptide immunomodulator "gepon" in experimental models of street rabies virus].intracerebral injections of the gepon drug were shown to be non-toxic when administered to outbred white mice, 0.01-0.1 micrograms. the drug protects 30-40% of mice (p < 95-99%) in contamination by street rables virus. the animals' protection depends on a dose and drug application scheme. the most pronounced protection was observed in its intramuscular administration, 0.1 microgram in the virus locus (infection opening). it should be necessarily pointed out that such pronounced protection (40%; ...200312945207
molecular and antigenic characterization of rabies viruses from iran identifies variants with distinct epidemiological origins.a molecular epidemiological study of 48 recent rabies isolates recovered from cases reported throughout iran identified three distinct viral variants, the evolutionary origins of which were identified by phylogenetic comparison with rabies viruses originating from europe and asia. members of group 1 (15 isolates) were recovered from the northern half of the country only, while those of group 2 (31 isolates) were widely dispersed; both groups clustered within the widely disseminated cosmopolitan ...200312948379
mapping of the low ph-sensitive conformational epitope of rabies virus glycoprotein recognized by a monoclonal antibody #1-30-44.monoclonal antibody (mab) #1-30-44 recognized an acid-sensitive conformational epitope of rabies virus glycoprotein (g). the antigenicity of g protein exposed on the cell surface was lost when the infected cells were exposed to ph 5.8. by comparing the deduced amino acid sequence of g protein between the hep-flury strain and the epitope-negative cvs strain as well as the mab-resistant escape mutants, two distant sites that contained lys-202 and asn-336 were shown to be involved in the epitope fo ...200312953844
use of discriminatory probes for strain typing of formalin-fixed, rabies virus-infected tissues by in situ hybridization.an in situ hybridization (ish) method has been developed to overcome difficulties encountered in the viral typing of formalin-fixed rabies virus-infected brain tissue. rabies viruses representative of all strains normally encountered in diagnostic submissions throughout canada, including 3 strains of terrestrial hosts (arctic fox, western skunk, mid-atlantic raccoon), 10 strains circulating in several bat reservoirs (bbcan1 to bbcan7, lacan, shcan, and mycan), and the evelyn-rokitniki-abelseth ( ...200312958267
incidence of adverse events in ferrets vaccinated with distemper or rabies vaccine: 143 cases (1995-2001).to determine the incidence of adverse events in ferrets vaccinated with a modified-live avian cell culture canine distemper virus vaccine licensed for use in ferrets, an inactivated rabies vaccine licensed for use in ferrets, or both.200312959385
rhabdoviruses and apoptosis.viral-induced apoptosis is recognized as a common method utilized by viruses to overcome the host. recent evidence indicates that infection by rhabdoviruses such as vesicular stomatitis virus (vsv), spring viremia of carp virus (svcv), and rabies virus results in apoptotic cell death. similar morphological changes and host cell proteins are induced in cells infected with these different viruses; however, the viral proteins responsible for these changes vary. in addition, the molecular mechanism( ...200312959754
cerebellar loops with motor cortex and prefrontal cortex of a nonhuman primate.we used transneuronal transport of neurotropic viruses to examine the topographic organization of circuits linking the cerebellar cortex with the arm area of the primary motor cortex (m1) and with area 46 in dorsolateral prefrontal cortex of monkeys. retrograde transneuronal transport of the cvs-11 (challenge virus strain 11) strain of rabies virus in cerebello-thalamocortical pathways revealed that the arm area of m1 receives input from purkinje cells located primarily in lobules iv-vi of the c ...200312968006
glycoprotein of nonpathogenic rabies viruses is a key determinant of human cell apoptosis.we showed that, unlike pathogenic rabies virus (rv) strain cvs, attenuated rv strain era triggers the caspase-dependent apoptosis of human cells. furthermore, we observed that the induction of apoptosis is correlated with a particular virus antigen distribution: the overexpression of the viral g protein on the cell surface, with continuous localization on the cytoplasmic membrane, and large cytoplasmic inclusions of the n protein. to determine whether one of these two major rv proteins (g and n ...200312970438
update on appropriate rabies postexposure prophylaxis. 200312971069
[symbiosis between the encephalitogenic strain of the coxsackie virus and the fixed rabies virus]. 195212976756
early appearance of rabies virus in the brain of hamsters exposed by rectal instillation. 195313010083
[hematological syndrome in white rats inoculated with fixed rabies virus]. 195213017185
[the effect of adrenalectomy in white rats inoculated with fixed rabies virus]. 195213017186
[search for rabies virus in rats of algiers]. 195213031712
virus encephalomyelitides. 195313036467
studies on the pathogenicity of an avianized street rabies virus. 195313067623
[experiments in vaccination of the dog with an attenuated rabies virus]. 195313081203
[contribution to the study of the flury rabies virus]. 195313082209
effect of rabies virus on mass and nucleic acids of embryonic nerve cells. 195313091874
[variation of the virulence of fixed rabies virus for the dog by intracerebral inoculation]. 195313093041
early appearance of rabies virus in the blood and in the brain of hamsters exposed by rectal instillation (preliminary report). 195313094928
studies on the purification of rabies virus. i. application of methanol precipitation and two other methods. 195313103149
rabies street virus strains in the syrian hamster. 195413114591
studies on chick-embryo-adapted rabies virus. iv. immunization of guinea-pigs and description of a potency control test. 195413118194
studies on chick-embryo-adapted rabies virus. v. protection of animals with antiserum and living attenuated virus after exposure to street strain of rabies virus. 195413118195
studies on chick-embryo-adapted-rabies virus. vi. further changes in pathogenic properties following prolonged cultivation in the developing chick embryo. 195413118196
[contribution to the study of the flury virus in rabies]. 195313119125
study of nine rabies street virus strains in the syrian hamster. 195313120888
particle size of soluble antigen of rabies virus. 195313121027
[the nature of the rabies virus]. 195313123837
[preservation of the rabbit adapted and nonadapted rabies virus in laboratory rodents]. 195313124937
[contribution to the study of the flury strain of rabies virus]. 195313139698
[contribution to the study of the flury rabies virus]. 195313144055
[appearance of negri bodies in the lesions produced by the fixed virus (strain louis pasteur) under the influence of hyaluronidase]. 195313148752
studies on leucomycin. iii. experimental treatment in animals with leucomycin. 195313152030
[effect of hyaluronidase on fixed virus]. 195413170328
response of the cave bat to several strains of rabies virus. 195413173150
studies on chick-embryo-adapted rabies virus. vii. immunological responses of animals to vaccination with high egg passage flury strain. 195413174815
on the action of the nitrogen mustard to rabies virus. 195313175380
biological modification of rabies virus as a result of its adaptation to chicks and developing chick embryos.this article describes experiments indicating a change in pathogenicity for laboratory animals of the flury strain of rabies virus at high egg passages. factors such as dilution of virus, number of egg passages, age of animals, and route of inoculation are taken into account. the results of the author's investigations indicate that living chick-embryo-adapted virus can be used both as a vaccine administered before exposure to rabies virus, and as an adjunct to antiserum in the protective treatme ...195413182592
treatment of wounds inflicted by rabid animals.this paper describes a series of experiments in which guinea-pigs were inoculated intramuscularly with a strain of fixed-virus rabies, and their wounds treated, after intervals of varying duration, with different viricidal substances. the authors found that cauterization with fuming nitric acid gave no greater protection against the development of rabies than did irrigation with a 20% soap-solution; a cationic detergent, zephiran, was found to be the treatment of choice for wounds artificially c ...195413182603
recent advances in the preparation of antirabies vaccines containing inactivated virus.this paper describes experiments undertaken to determine the usefulness of 15 nitrogen-mustard and mustard-like drugs in inactivating fixed rabies virus for the preparation of experimental antirabies vaccines. one or more of the five agents eventually selected gives promise of practical value in rendering rabbit-brain fixed rabies virus and duck-embryo fixed rabies virus noninfective for mice, at the same time allowing of successful antirabies immunization.195413182604
avianized rabies virus vaccination in man.this paper describes the results of three experiments in which 98 volunteers were inoculated intramuscularly with living avianized rabies virus of the flury strain, modified by adaptation to chick embryo; their reactions were compared with those of 31 other persons either inoculated with normal-embryo material, or undergoing pasteur treatment. immune response was estimated by determination of the development of neutralizing antibodies. these observations show that flury-virus vaccine can be used ...195413182605
recovery of rabies virus from colonial bats in texas. 195413186061
[flury rabies virus]. 195413190275
[flury strain of rabies virus]. 195413193166
[not available]. 195413193330
[inoculation of flury rabies virus in monkey]. 195413197863
[study of the flury rabies virus. ii..]. 195413198291
[culture of rabies virus in vitro]. 195413209901
[inoculation of flury rabies virus into the anterior chamber of the eye of rabbits]. 195413218365
[biological properties of the flury strain of rabies virus]. 195413218606
[dormant form of rabies virus of flury strain in rabbit]. 195413229392
tissue culture, virus propagation, and viral chemotherapy. 195413234058
studies on the purification of rabies virus by the methanol precipitation. 195413242254
[necessity of a conference on the flury rabic virus and the antirabic serum]. 195513244060
rabies in cattle. iv. vaccination of cattle with high egg-passage, chicken embryo-adapted rabies virus. 195513263233
interference between variants of pseudorabies virus demonstrable in tissue culture. 195513272731
transmission of street rabies virus strain (v308) to suckling hamsters during lactation. 195513273430
transmission of a street rabies virus strain (r-174) from the cave bat (myotis lucifugus) to the tick (ornithodoros) by blood feeding: a preliminary report. 195613277316
[rabies virus flury in rabbit]. 195513283615
[need for a restricted conference on rabies virus flury and on antirabies vaccine]. 195513283616
[titration of virulence of rabies virus in dogs]. 195513283617
[electron photography of the fixed virus of rabies]. 195513302872
[multiplication of rabies virus fixed and street viruses in dialyzed tissue cultures]. 195613305009
[affinity of rabies virus for the cells of kidney origin cultivated in vitro]. 195613316281
[first results of the study of action of various hormones and of sodium nucleinate on the rabies virus]. 195613317135
[essay with culture of common rabies virus]. 195613327486
[use of white mice in determination of rabies virus]. 195613332887
[human rabies caused by fixed virus; first verification in argentina]. 195613350009
[electron microscopic examination of rabies virus]. 195613352208
[etiology of dikovanie in animals in the polar region. i. biological properties of dikovanie virus]. 195613352972
[cultivation of rabies virus in developing chick embryo]. 195613352973
isolation of rabies virus from insectivorous bats in yugoslavia. 195613356149
[antigenic interrelationship between strains of common rabies virus of various origins and fixed rabies virus]. 195613361190
[flury virus]. 195613373453
Displaying items 2901 - 3000 of 5712