| n-methyl-l-glutamate synthase. | | 1985 | 3911004 |
| [study of the sensitivity of anaerobic bacteria using 2 dilution technics]. | we studied forty-seven anaerobic strains recovered from blood cultures or pus from deep infections over three months. antibiotic sensitivity was tested using two dilution techniques. for both, a standardized, controlled inoculum was used and strains with known susceptibilities were included. the first method consisted of dilution of antibiotics in schaedler broth and mic determination using microplates. in the second method, dilutions in wilkins-chalgren broth with 1.5 g/l agar and atb-api plate ... | 1985 | 3911155 |
| photochemical modifications of lac repressor: effect of effectors binding on tryptophan photooxidation. | | 1985 | 3911222 |
| [significance of isolating shigella under epidemiological circumstances]. | | 1985 | 3911318 |
| viruses and immune reactions in the liver. | the immunopathology of hepatitis b and delta virus infections of the liver are reviewed. it is clear there are several antigen/antibody systems of importance in acute and chronic hbv infection. antibodies to hbs, hb core/hbe and antibodies to dane specific determinants are involved in virus neutralisation. elimination of virus infected hepatocytes is dependent on recognition of viral determinants in association with hla proteins on the infected hepatocytes by cytotoxic t cells. the hla protein d ... | 1985 | 3911368 |
| the potential dependence of the intestinal na+-dependent sugar transporter. | the unidirectional influx of the lipophilic cation tetraphenylphosphonium (tpp+) into isolated intestinal epithelial cells exhibits a marked dependence on the membrane potential (delta psi) maintained by or experimentally imposed on these cells. by taking advantage of this fact, we have described a "crossover" procedure that allows the relative permeability of a cation and anion pair to be determined. measurements of such relative permeabilities permits diffusion potentials of defined magnitude ... | 1985 | 3911844 |
| [preventing bacillary dysentery with magnetized drinking water]. | | 1985 | 3912058 |
| [epidemiologic survey of an outbreak of food-borne bacillary dysentery]. | | 1985 | 3912060 |
| plasmid characterization of shigella spp. isolated from children with shigellosis and asymptomatic excretors. | strains of shigella dysenteriae type 1 and sh. flexneri isolated during 1981-82 from children with shigellosis and also from asymptomatic excretors were examined for drug resistance and for the presence of plasmids. shigella strains isolated from dysenteric children showed multiple plasmid bands. thirty-eight percent of the strains transferred the drug resistance factor(s), either partially or fully to a recipient escherichia coli k12 strain. plasmids in the molecular weight range from between 4 ... | 1985 | 3912364 |
| shigella flexneri bacteraemia in a patient with acquired immune deficiency syndrome. | | 1985 | 3913261 |
| studies on specificity of protection induced by immunization with outer membrane proteins of shigella. | immunization of c3h/hej mice with outer membrane proteins (omp) and with peptidoglycan associated proteins (pgp) isolated from shigella flexneri 3a and from shigella sonnei phase i protected the animals against lethal dose of homologous and heterologous bacteria and against various serotypes of shigella flexneri. neither of the protein preparations protected the animals against challenge with escherichia, klebsiella, citrobacter, salmonella, serratia, proteus and pseudomonas. omp preparations ho ... | 1985 | 3914866 |
| transfer of immunity by means of spleen cells from mice immunized with outer membrane proteins of shigella flexneri. | intraperitoneal immunization of mice with a single dose (5 micrograms) of outer membrane proteins (omp) of shigella flexneri was found to evoke in the spleen the appearance of cells by means of which immunity to lethal dose of shigella could be transferred into other mice. active cells capable of transferring immunity appeared in the spleen of the animals as early as on day 3, reached the strongest protective activity on day 4 and disappeared on day 8 after immunization. active cells from animal ... | 1985 | 3914867 |
| [emergence of resistance to antibiotics in enteropathogens. implications and speculations]. | | 1985 | 3915679 |
| emergence of sulfamethoxazole-trimethoprim resistant shigella flexneri in northeastern brazil. | in contrast to prior experience in this setting, three of four shigella flexneri strains recently isolated from patients in northeastern brazil with acute inflammatory diarrhea were found to be resistant to sulfamethoxazole, trimethoprim and the combination in vitro. we performed mating studies to determine if the resistance was transferable, and then isolated and characterized plasmid dna from the resistant shigella isolates, other resistant enterobacteriaceae isolated simultaneously from the s ... | 1985 | 3915680 |
| systemic mycosis in scottish red deer (cervus elaphus). | the pathological features of 10 cases of systemic infection of young red deer by fungi morphologically identified as phycomycetes are described. the most frequently affected organs were the kidneys, followed by liver and central nervous system. the fungi stimulated an acute pyogenic inflammatory reaction and a phagocytic giant cell response. vasculitis with thrombosis and spread of fungi to the surrounding tissue was a common finding. culture was attempted in 4 cases; one yielded absidia, 2 aspe ... | 1985 | 3915751 |
| [shigellemia in a patient with the septic variant of the course of dysentery (clinical case)]. | | 1985 | 3916350 |
| cell-mediated immunity in type i (insulin-dependent) diabetes of man and the bb rat. | | 1985 | 3916413 |
| [effect of sulfonamides on the fatty acid composition of enterobacterial lipids]. | | 1985 | 3917183 |
| inefficacy of penicillin v in acute laryngitis in adults. evaluation from results of double-blind study. | patients with acute laryngitis following an upper respiratory tract infection are often treated with antibiotics for their voice complaints, although, to our knowledge, the effect of such therapy has not been examined. in the present study, comprising 100 adults with laryngitis, the rate of resolution of vocal symptoms, as estimated from voice recordings or subjectively by the patients, was the same in patients who received penicillin v (pcv) as in those who received placebo. similarly, the degr ... | 1985 | 3918495 |
| in vivo efficacy and ultrastructural effects of mitomycin c against experimental alveolar hydatid disease. | the in vivo efficacy and ultrastructural effects of mitomycin c were determined against alveolar hydatid disease in experimentally infected animals and compared to mebendazole treatment. mitomycin c inhibited the mean cyst mass of treated versus control animals by 84.1% which was statistically significant at the alpha = 0.01 level. mebendazole given daily inhibited the mean cyst mass by 80.1%, while mebendazole administration on the same treatment schedule as that used for mitomycin c inhibited ... | 1985 | 3921601 |
| effect of human gamma interferon on invasiveness of salmonella typhimurium in hep-2 cell cultures. | the effect of human gamma interferon (huifn-gamma) on the invasiveness of salmonella typhimurium, shigella flexneri and yersinia enterocolitica serotype 0:3 in hep-2 cell cultures was examined. the intracellular and extracellular bacteria were identified by a combination of nomarski differential interference contrast microscopy and uv incident light microscopy applied on the same microscope. pretreatment of hep-2 cells with huifn-gamma reduced in a dose dependent manner the number of s. typhimur ... | 1985 | 3921632 |
| properties of human anti-lipopolysaccharide gamma globulin: specificity and protective effects. | blood donated to the natal blood transfusion service was screened by an enzyme-linked immunosorbent assay (elisa) for anti-lipopolysaccharide (anti-lps) antibodies. plasma units with high concentrations (greater than 40 micrograms/ml) of anti-lps igg were pooled and fractionated to obtain a gamma globulin (lot lg-1). the binding of lg-1 antibodies to lps prepared from 14 bacterial species and strains was found to be the highest to lps from shigella flexneri, salmonella abortus equi and salmonell ... | 1985 | 3922117 |
| isolation and characterization of a new endo-beta-galactosidase from diplococcus pneumoniae. | an endo-beta-galactosidase, which hydrolyzes the internal beta-galactosidic linkages of r----glcnac (or galnac) beta 1----3gal beta 1----4glcnac (or glc), was isolated from the culture supernatant of diplococcus pneumoniae. the enzyme, named endo-beta-galactosidase dii, hydrolyzed linear n-acetyllactosamine repeating structures in glycolipids and glycopeptides to release oligosaccharides. the specificity of endo-beta-galactosidase dii is the same as that of escherichia freundii endo-beta-galacto ... | 1985 | 3922410 |
| pressure- and temperature-dependent adhesion of pseudomonas aeruginosa to hep-2 cells. | pseudomonas aeruginosa bacteria adhere to human epitheloid (hep-2) cells in culture. under normal atmospheric conditions (1 ata), this adhesion increased significantly when the temperature rose from 22 to 37 degrees c. under hyperbaric atmosphere (= air, 7 ata) conditions, a similar, significant enhancement of bacterial adhesion to the cells was noted when the temperature rose. if the temperature was kept stable at 22 or 37 degrees c and the pressure was increased from 1 to 7 ata, a pressure-ind ... | 1985 | 3925713 |
| radioimmunoassay of thyroxine in saliva. | a simple radioimmunoassay (ria) for thyroxine (t4) in saliva has been described. fifty euthyroid control subjects, 14 euthyroid pregnant women, 23 thyreotoxic and 10 hypothyroid patients were examined. serum t3, t4, thyroxine binding globulin (tbg) and tsh were measured simultaneously. the mean level of t4 in saliva in controls was 1.10 +/- 0.07 nmol/l. there was a good correlation between the saliva and serum t4 concentrations (r = 0.74) and between saliva t4 values and the t4/tbg ratio (r = 0. ... | 1985 | 3926523 |
| fractionation of radioactivity in the milk of goats administered 14c-aflatoxin b1. | a detailed fractionation of radioactivity in the milk of goats administered 14c-aflatoxin b1 at low doses was performed. the milk collected in the first 24 h following dosing contained radioactivity equivalent to 0.45-1.1% of the dose given. the radioactivity in each sample was partitioned into 4 fractions: ether, protein, dichloromethane, and water-alcohol. over 80% of the radioactivity was detected in the dichloromethane fraction, of which over 95% was attributable to aflatoxin m1. no aflatoxi ... | 1985 | 3926742 |
| fractionation of radioactivity in the milk of goats administered 14c-aflatoxin b1. | a detailed fractionation of radioactivity in the milk of goats administered 14c-aflatoxin b1 at low doses was performed. the milk collected in the first 24 h following dosing contained radioactivity equivalent to 0.45-1.1% of the dose given. the radioactivity in each sample was partitioned into 4 fractions: ether, protein, dichloromethane, and water-alcohol. over 80% of the radioactivity was detected in the dichloromethane fraction, of which over 95% was attributable to aflatoxin m1. no aflatoxi ... | 1985 | 3926742 |
| improved control in advanced head and neck cancer with simultaneous radiation and cisplatin/5-fu chemotherapy. | chemotherapy with cisplatin plus 5-fu infusion was given simultaneously with radiation therapy; both were administered every other week. twenty-seven patients with stage iv disease and three with stage iii disease were treated; 57% had t4, 33% had n3, and 10% had m1 disease. of these patients, 17 (57%) achieved complete response and the rest achieved partial response. however, whether or not a clinically complete response occurred had no effect on subsequent risk for disease recurrence. with a m ... | 1985 | 3928158 |
| in vitro pyocin activity of pseudomonas aeruginosa strains pretreated with antibiotics. | fifty-six selected strains of pseudomonas aeruginosa belonging to 8 different pyocin types (h, i, 15, 6, pti-1, pti-2, pti-3, pti-4) were treated with subinhibitory concentrations (mic/2) of either gentamicin or carbenicillin. both treatments induced changes in pyocin patterns for all types but at different levels. the percentage of strains that retained their pyocin pattern were more or less equal in both treatments. in treated and untreated producers, the growth inhibition ability for 5 differ ... | 1985 | 3928466 |
| clues to the puzzle of purpose. | | 1985 | 3929138 |
| [study on an intestinal loop test of virulent shigella bacillus]. | | 1985 | 3932548 |
| selected overview of nongynecologic surgical intra-abdominal infections. prophylaxis and therapy. | true prophylaxis of intra-abdominal nongynecologic infections is limited to elective, nonemergency surgery and is best shown in three clean-contaminated surgical procedures. all of these have an infection rate of approximately 10 to 20 percent and include all colon resection surgery, most gastric surgery, and about one third of the cholecystectomies for chronic calculous cholecystitis. each of these three surgical procedures has a somewhat different pattern of bacterial pathogens. the most usefu ... | 1985 | 3934966 |
| [enhanced effectiveness of antibiotic therapy with an exogenous lysozyme in dysentery in children]. | the course of dysentery and the host immune responsiveness were studied in 171 children with acute flexner's and sonne dysentery. 67 children were treated with monomycin in combination with lysozyme and 104 children with monomycin alone. it was shown that the recovery period in children treated with monomycin and lysozyme was shorter as compared to that in children treated with monomycin alone. the immunity characteristics in children treated with monomycin were closer to the physiological value ... | 1985 | 3936408 |
| evaluation of chemotherapy with benznidazole and nifurtimox in mice infected with trypanosoma cruzi strains of different types. | a test was made of the susceptibility of 30 strains of trypanosoma cruzi to chemotherapy with nifurtimox (bay 2502) and benznidazole (ro 7-1051). the strains had previously been classified as type i, ii, or iii according to their morphobiological and isoenzymic characteristics. three type i strains, 14 type ii strains, and 13 type iii strains were studied. mice were infected with 2 x 10(5) blood forms of these parasites and treated for 90 days with benznidazole or nifurtimox. all the surviving m ... | 1985 | 3936634 |
| chlamydial proctitis? | chlamydia trachomatis was isolated from 21 (7%) of 309 specimens obtained in october 1982 from the rectum of homosexual men undergoing proctoscopy. during the same period neisseria gonorrhoeae was isolated from 12 (3%) of 454 specimens obtained similarly. the clinical features of patients infected with each of these micro-organisms were compared with those of an uninfected group of homosexual men. no characteristic diagnostic features were noted in the infected men. | 1985 | 3936777 |
| arteriovenous flow reversal, experimental investigations. i. complete flow reversal of the small intestine and of the rear extremity of the dog. | segments of the small intestine and hind extremities of dogs were perfused under the condition of complete arterio-venous flow reversal (avfr). in the intestinal segments, the peripheral resistance under avfr conditions was 4.4 times higher than under orthograde perfusion. petechiae and hemorhagic infarction were observed. the average flow resistance of the hind extremities was 2.4 times higher than the resistance in control extremities. the po2 and the o2 saturation in the blood, outflowing fro ... | 1985 | 3937361 |
| assessment by a two-site enzyme immunoassay of human epidermal growth factor (urogastrone) in the urine of patients with various gastrointestinal diseases including malignant tumors. | by using our two-site enzyme immunoassay (eia) system, the levels of human epidermal growth factor (hegf) in the urine of patients with various gastrointestinal diseases including malignant tumors were measured. urinary excretion of hegf in patients having undergone gastric resection, expressed as a function of creatinine, was found to be somewhat decreased. while the levels of hegf in patients with gastric cancer were significantly increased. then, the molecular features of hegf in the urine of ... | 1985 | 3937528 |
| initiation and promotion in young and old animals. implications for human tumour formation. | the significance of ageing in experimental skin carcinogenesis and on the manifestation of tumour formation in man was studied. initiation with the carcinogenic hydrocarbon 7,12-dimethylbenz [a]anthracene and promotion with croton oil in mouse skin showed that tumour formation was unchanged when promotion was delayed 3, 10, 23 or 43 weeks in comparison with that in untreated animals of the same age. the sensitivity to carcinogen treatment of animals over 71 weeks of age was less than that of ani ... | 1985 | 3938448 |
| [virulence of shigella: epithelial cell invasiveness and other dysentery-producing factors]. | | 1985 | 3938489 |
| ostertagia ostertagi: excretory secretory chemotactic substance from infective larvae as cause of eosinophil locomotion. | the infective (third stage) larva of ostertagia ostertagi produced an excretory secretory substance that is chemotactic for bovine eosinophils. the eosinophil chemotactic substance was present in supernatants of larval cultures within 6 hr of incubation in eagle's minimum essential medium containing 25 mm n-2-hydroxyethylpiperazine-n'-2-ethanesulfonic acid. the substance responsible for eosinophil chemotaxis has activity in small amounts, a molecular weight (mw) greater than 2000, and is heat la ... | 1986 | 3943588 |
| ostertagia ostertagi: excretory secretory chemotactic substance from infective larvae as cause of eosinophil locomotion. | the infective (third stage) larva of ostertagia ostertagi produced an excretory secretory substance that is chemotactic for bovine eosinophils. the eosinophil chemotactic substance was present in supernatants of larval cultures within 6 hr of incubation in eagle's minimum essential medium containing 25 mm n-2-hydroxyethylpiperazine-n'-2-ethanesulfonic acid. the substance responsible for eosinophil chemotaxis has activity in small amounts, a molecular weight (mw) greater than 2000, and is heat la ... | 1986 | 3943588 |
| trichinella spiralis: newborn larval migration route in rats reexamined. | the route by which trichinella spiralis newborn larvae migrate from the small intestine to striated muscle was studied in inbred ao and random-bred sprague-dawley rats. newborn larvae were quantitatively recovered from the thoracic duct lymph, peritoneal cavity, and hepatic portal vein blood during the course of a primary infection with 4000 muscle larvae. the total recovery of newborn larvae assessed in this manner was compared with the number of muscle larvae in control rats receiving the same ... | 1986 | 3943594 |
| on dying more than one death. | death (with a capital d) can best be understood as a series of distinct but related deaths. for example, a pregnant woman was found to be brain-dead but her vital functions were artificially sustained for nine weeks until her fetus could be delivered, after which the machines were removed and she died a second, conceptually distinct death. this procedure is probably justifiable, but any legislation or policy regarding such cases should be flexible and should require consent. | 1986 | 3957650 |
| [secondary cutaneous mycoses caused by alternaria species]. | analysis of 2 of our own cases of cutaneous alternariosis (ca) and 31 reported cases showed this mycosis to be nosologically heterogeneous. ca may present as an endogenous, multilocular form or as an exogenous, usually localized form. in the endogenous form of ca (11 cases), infection most probably occurs via the lung by inhalation. the highly dermatotropic organisms spread hematogenically to the skin where they cause the typical verruciform or granulomatous lesions. in the exogenous form (8 cas ... | 1986 | 3957668 |
| retinal constraints on orientation specificity in cat visual cortex. | most retinal ganglion cells (levick and thibos, 1982) and cortical cells (leventhal, 1983; leventhal et al., 1984) subserving peripheral vision respond best to stimuli that are oriented radially, i.e., like the spokes of a wheel with the area centralis at the hub. we have extended this work by comparing directly the distributions of orientations represented in topographically corresponding regions of retina and visual cortex. both central and peripheral regions were studied. the relations betwee ... | 1986 | 3958796 |
| [the systematic evaluation of medical procedures. apropos of the effectiveness of parallel medicine]. | | 1986 | 3961360 |
| the distribution of acetylcholine and choline in guinea pig heart. | the regional distributions of acetylcholine (ach) and choline (ch) in the guinea pig heart were investigated with a pyrolysis-mass fragmentography technique. using ach as a marker for cholinergic neurons, we have described a pattern of parasympathetic innervation in the guinea pig heart. this distribution is very similar to that suggested by studies using several different cholinergic indicators in various species. atrial areas receive richer parasympathetic innervation than ventricular areas, w ... | 1985 | 3965854 |
| characterisation of a dictyostelium discoideum dna fragment coding for a putative trnavalguu gene. evidence for a single transcription unit consisting of two overlapping class iii genes. | a genomic dna fragment from dictyostelium discoideum was characterized. this dna, although 74% d(a + t)-rich, codes for a putative trnavalguu. the trnaval gene overlaps at its 5' half with another rna polymerase iii transcription unit. this rna polymerase iii transcription unit can be folded into a trna-like shape and is comprised of significant amounts of invariant and semi-invariant nucleotides present in all eukaryotic trnas. this unit contains the two promoter blocks defined for rna polymera ... | 1985 | 3967666 |
| development and morphology of rat synovial membrane. | the development of the knee joint and synovial membrane in wistar rats was studied from the 13th fetal day until adulthood by means of light microscopy, transmission electron microscopy and scanning electron microscopy. using acid phosphatase and peroxidase methods, the different cell types of synovial membrane could be identified. | 1985 | 3969821 |
| structural identification of platelet activating factor in psoriatic scale. | platelet activating factor was isolated from the lesional scale of psoriatic patients using the method described by bligh and dyer (8). the extract was subjected to thin layer chromatography, and the region of the plate co-migrating with platelet activating factor removed. a portion of each sample was assayed for aggregating activity using washed guinea-pig platelets and the remainder treated with phospholipase c, derivatised, and subjected to reversed phase high performance liquid chromatograph ... | 1985 | 3970690 |
| spatial contrast sensitivity deficits in monkeys produced by optically induced anisometropia. | an anisometropia was simulated in infant rhesus monkeys by securing a high-powered minus lens (-10 d) in front of one eye. the anisometropia rearing procedure was initiated at 30 days of age and was continued for durations of 30, 60, or 90 days. behavioral measurements of spatial contrast sensitivity obtained when the animals were 9 months of age indicated that the monkeys treated for 30 days had equal or nearly equal contrast sensitivities and cut-off spatial frequencies in the two eyes. the 30 ... | 1985 | 3972513 |
| synthesis of bunyavirus-specific proteins in a continuous cell line (xtc-2) derived from xenopus laevis. | the xtc-2 cell line, derived from xenopus laevis, supported the replication of representative viruses from each of the four genera in the family bunyaviridae. generally, viral titres were higher in xtc-2 cells than in other susceptible cell lines, and for some viruses plaques were detected earlier in xtc-2 cells. the xtc-2 cell line permitted comparative analyses of bunyavirus-specific protein synthesis. the patterns of synthesis of viral proteins, characteristic of each of the genera, were obse ... | 1985 | 3973561 |
| effects of neuronal activity on inositol phospholipid metabolism in the rat autonomic nervous system. | the effect of nerve stimulation on inositol phospholipid hydrolysis in autonomic tissue was assessed by direct measurement of [3h]inositol phosphate production in ganglia that had been preincubated with [3h]inositol. within minutes, stimulation of the preganglionic nerve increased the [3h]inositol phosphate content of the superior cervical sympathetic ganglion indicating increased hydrolysis of inositol phospholipids. this effect was blocked in a low ca2+, high mg2+ medium. it was also greatly r ... | 1985 | 3973589 |
| noninvasive measurement of pressure/volume characteristics in human limb veins by electrical admittance plethysmography. | | 1985 | 3974317 |
| [effects of porta-systemic shunts on the liver]. | | 1985 | 3979196 |
| chronic cutaneous granulomatous dermopathy: morphologic and serologic evaluation, differential diagnosis and pharmacologic control. | we report on a syndrome of disfiguring lower extremity cutaneous lesions of 6 years duration. previous medical evaluations included 5 skin biopsies and several therapeutic regimens which did not alter the course of illness. the histologic interpretations of skin biopsies included panniculitis, papulonecrosis, and granuloma. one biopsy was interpreted as a localized cutaneous vasculitis with a wedge-shaped infarct. infectious, collagen vascular, and factitious etiologies could not be demonstrated ... | 1985 | 3980110 |
| complement and hla. further definition of high-risk haplotypes in insulin-dependent diabetes. | the families of 41 probands with type i (insulin-dependent) diabetes mellitus (iddm) were typed for hla-a, hla-b, and hla-dr antigens in addition to the complement polymorphisms c2, c4a, c4b, and bf. all of these loci are encoded on the short arm of human chromosome 6 in a narrow region. alleles at hla-b (8, 15, 18, and 40), hla-dr (3 and 4), and bf (f1) have been associated with increased relative risk (rr) for iddm, while hla-b7 and hla-dr2 have been associated with decreased rr for iddm. this ... | 1985 | 3987976 |
| the effect of intermittent lighting on growth, feed:gain ratio, and abdominal fat content of broiler chickens of various genotypes and sex. | intermittent lighting (il) regimens were tested for rearing broiler chickens from 2 to 7 weeks of age. in experiments 1 and 2, the effects of 6 cycles of light (l) and darkness (d) of 1l:3d vs. 12 cycles of .25l:1.75 d were compared on the performance of 2400 broiler chickens. eight genotypes of 5170 mixed-sex broilers were used to compare effects of 6 cycles of 1l:3d vs. 1 cycle per day of 23.5l:.5d on broiler performance (experiment 3). the sexes responded differently to two il regimens in exp ... | 1985 | 3991421 |
| [persistent chlamydia infection in cell culture]. | | 1985 | 3993192 |
| plasma prolactin in the rat during suckling without prior separation from pups. | the relation between suckling and plasma prolactin (prl) was studied in the rat, without prior separation of the dam from its pups. when the pups were replaced by a hungry foster litter, upon renewed suckling plasma prl showed episodic increases and decreases in individual rats. when, subsequent to litter removal, similar rats were injected with perphenazine, a significant increase of plasma prl was observed. this indicates that a decline of plasma prl during suckling was not caused by exhaustio ... | 1985 | 3993313 |
| the corneal endothelial cell interface. | the apical edges of corneal endothelial cells have been separated along lines forming a crisscross pattern, by the help of a special method of preparation. the visualization by sem of the lateral faces of the cells is thereby made possible. the separation leads to the formation of thin strands spanning the gap between the apical edges of adjoining cells. they are formed by stretching of the normal apical flaps. this stretching demonstrates that the main intercellular adherence is located at the ... | 1985 | 3993338 |
| the acquired immunodeficiency syndrome (aids) in denmark. a report from the copenhagen study group of aids on the first 20 danish patients. | twenty danish patients with the acquired immunodeficiency syndrome (aids) had been diagnosed by january 1984, 14 of them after 1982. eighteen patients were male homosexuals, 8 of whom had visited the usa after 1979, 2 were heterosexual males with a history of sexual contacts in central africa, suggesting a transmission of aids from woman to man. aids has not been observed in drug abusers, hemophiliacs or transfused non-risk persons in denmark. the clinical picture varied according to the presenc ... | 1985 | 3993435 |
| longitudinal study of salt preferences in normotensive and hypertensive rats. | to determine whether age-related changes in salt preferences occur over the lifespans of spontaneously hypertensive rats (shr) and wistar-kyoto rats (wky), the same animals of each genotype were tested as juveniles, and as young and older adults. taste preference ratios for nacl and kcl, at concentrations from 0.001 to 1.0 m, were calculated using 24-hour, two-bottle preference tests of each salt versus distilled water. genotype exerted a significant effect on preference for both nacl and kcl (p ... | 1985 | 3997218 |
| longitudinal study of salt preferences in normotensive and hypertensive rats. | to determine whether age-related changes in salt preferences occur over the lifespans of spontaneously hypertensive rats (shr) and wistar-kyoto rats (wky), the same animals of each genotype were tested as juveniles, and as young and older adults. taste preference ratios for nacl and kcl, at concentrations from 0.001 to 1.0 m, were calculated using 24-hour, two-bottle preference tests of each salt versus distilled water. genotype exerted a significant effect on preference for both nacl and kcl (p ... | 1985 | 3997218 |
| cadmium chloride susceptibility, a characteristic of campylobacter spp. | we report a simple diagnostic characteristic useful in the presumptive identification of campylobacter jejuni and campylobacter coli. filter paper disks impregnated with cadmium chloride were placed on streaked agar medium. zones of growth inhibition for campylobacter spp. occurred at 1.25 micrograms per disk. other enteropathogens (salmonella spp., shigella spp., vibrio cholerae, vibrio parahaemolyticus, escherichia coli, and yersinia enterocolitica) were resistant to at least 40 micrograms per ... | 1985 | 3998099 |
| complement activation and clearance in acute illness and injury: evidence for c5a as a cell-directed mediator of the adult respiratory distress syndrome in man. | the appearance of the adult respiratory distress syndrome (ards) during the course of acute illness is believed to result, in part, from intrapulmonary neutrophil sequestration and degranulation induced by circulating inflammatory mediators. to evaluate the role of complement-neutrophil interactions in the pathogenesis of ards in man, 34 patients suffering from intra-abdominal sepsis (seven), multisystem trauma (15), or acute pancreatitis (12) were serially studied with regard to neutrophil migr ... | 1985 | 4002115 |
| hemodilution during standardized hemorrhage in high-altitude acclimatized rats. | ten control rats and sixteen high-altitude acclimatized rats were bled at sea level into a reservoir which maintained arterial pressure at 35 mm hg. as soon as the animals had spontaneously taken back 30% of the maximum bleeding volume, all the shed blood remaining in the reservoir was reinfused. hemodilution was studied during the first half phase of hypotension starting from the point of initial blood withdrawal and ending at the point of maximum blood loss. changes in hematocrit, hemoglobin c ... | 1985 | 4004677 |
| hemodilution during standardized hemorrhage in high-altitude acclimatized rats. | ten control rats and sixteen high-altitude acclimatized rats were bled at sea level into a reservoir which maintained arterial pressure at 35 mm hg. as soon as the animals had spontaneously taken back 30% of the maximum bleeding volume, all the shed blood remaining in the reservoir was reinfused. hemodilution was studied during the first half phase of hypotension starting from the point of initial blood withdrawal and ending at the point of maximum blood loss. changes in hematocrit, hemoglobin c ... | 1985 | 4004677 |
| 31p-saturation-transfer nuclear-magnetic-resonance measurements of phosphocreatine turnover in guinea-pig brain slices. | the technique of 31p saturation-transfer n.m.r. was used to determine the forward and the reverse rate constants of creatine phosphotransferase in superfused guinea-pig cerebral tissues in vitro. the calculated forward rate constant of 0.22 +/- 0.03s-1 compared well with a previously reported value for rat brain in vivo [shoubridge, briggs & radda (1982) febs lett. 140, 288-292]. the reverse rate constant was found to be 0.55 +/- 0.10s-1. 3. by using concentrations of atp and phosphocreatine est ... | 1985 | 4004799 |
| lack of effect of intranigral administration of a dopamine analogue, (+/-)-3-(3-hydroxyphenyl)-n,n-propylpiperidine [(+/-)-3-ppp], on nigrostriatal dopamine neurones. | bilateral injection of apomorphine into the zona reticulata of substantia nigra in rats reduced locomotor activity and striatal dopamine (da) turnover. in contrast, bilateral injection of (+/-)-3-(3-hydroxyphenyl)-n,n-propylpiperidine [(+/-)-3-ppp] into the zona reticulata (zr) did not reduce locomotor activity and did not affect striatal da turnover. a high dose of (+/-)-3-ppp increased locomotor activity. unilateral injection of (+/-)-3-ppp, but not of apomorphine, into the zr induced a brief ... | 1985 | 4011049 |
| [mechanism of the anti-arrhythmic action of phosphocreatine in acute myocardial ischemia]. | the association between phosphocreatine's antifibrillatory action and its effect on the excitement propagation processes in the ischemic area was investigated under acute coronary arterial occlusion in dogs. ischemia considerably reduced the amplitude, and increased the duration and time of onset, in local electrograms, and provoked cardiac fibrillation at the time of occlusion or during the recovery of coronary flow. a single intravenous injection of 300 mg/kg phosphocreatine eliminated cardiac ... | 1985 | 4021280 |
| isolation and sequence of cdna clones coding for a member of the family of high mobility group proteins (hmg-t) in trout and analysis of hmg-t-mrna's in trout tissues. | a specific oligonucleotide has been used to isolate a cdna prepared from the mrna for a trout high mobility group (hmg) protein closely related to trout hmg-t and bovine hmg 1 and 2 proteins. the sequence isolated more closely resembles bovine hmg-1 than the previously sequenced hmg-t protein in regions corresponding to the n terminal half of the protein. northern blot analysis at low stringency indicated that 2 related sequences are expressed in total trout testis mrna. southern blots of total ... | 1985 | 4022777 |
| tenckhoff catheter obstruction resulting from invasion by curvularia lunata in the absence of peritonitis. | a 60-year-old black woman on continuous ambulatory peritoneal dialysis (capd) presented with tenckhoff catheter malfunction. she exhibited no signs or symptoms of acute peritonitis. darkly pigmented plaques were adherent to the inner wall of the catheter and exchange tubing focally throughout its length. similar material was adherent to the protein coagulum in the dialysis bag. the tenckhoff catheter and tubing were removed, and hemodialysis was initiated. cultures of the catheter and the dialys ... | 1985 | 4025332 |
| identification of urinary metabolites of the nephrotoxic hydrocarbon 2,2,4-trimethylpentane in male rats. | the compound 2,2,4-trimethylpentane (2,2,4 tmp) is reported to be especially potent in inducing kidney lesions in male rats (1,2). although the pathology produced by 2,2,4 tmp has been examined (1), there are no reports concerning the metabolism of 2,2,4 tmp by the male rat. the eight principal urinary metabolites of 2,2,4 tmp found in the urine of fischer 344 male rats are: 2,2,4-trimethyl-1-pentanol, 2,4,4-trimethyl-1-pentanol, 2,4,4-trimethyl-2-pentanol, 2,2,4-trimethyl-1-pentanoic acid, 2,4, ... | 1985 | 4026835 |
| [characteristics of a cell line obtained from a human hypernephroma]. | a stable tumorigenic cell line has been obtained from the human hypernephrome. the culture grew as monolayers of large polygonal cells with foamy cytoplasm and 1-2 nucleoli. the cells formed monolayer after the third day after planting into flask and then formed multilayered growth. the electron microscopic studies of hypernephrome sections revealed the dark and light cells. the hypernephrome cells have characteristics of tumor cells. on the 200th passage the cells are aneuploid with a model num ... | 1985 | 4027291 |
| the effect of histone h1 on the compaction of oligonucleosomes. a quasielastic light scattering study. | the structural properties of h1-depleted oligonucleosomes are investigated by the use of quasielastic laser light scattering, thermal denaturation and circular dichroism and compared to those of h1-containing oligomers. to obtain information on the role of histone h1 in compaction of nucleosomes, translational diffusion coefficients (d) are determined for mono-to octanucleosomes over a range of ionic strength. the linear dependences of d on the number of nucleosomes show that the conformation of ... | 1985 | 4027333 |
| outbreak of shigella flexneri 1 infection in british columbia. | | 1985 | 4027813 |
| myocardial recovery after hypothermic arrest: a comparison of oxygenated crystalloid to blood cardioplegia. the role of calcium. | we compared multidose crystalloid hyperkalemic cardioplegic solutions with and without added red cells in 24 canine hearts subjected to 5 hr of arrest at 10 degrees c. all cardioplegic solutions were fully oxygenated at 4 degrees c before delivery. since blood cardioplegia contained ca++ carried over with the red cells, ca++ was added to the crystalloid solution in one group. the table below shows the hematocrit (hct) and ionized ca++ concentrations of the cardioplegic solutions, and coronary ar ... | 1985 | 4028363 |
| myocardial recovery after hypothermic arrest: a comparison of oxygenated crystalloid to blood cardioplegia. the role of calcium. | we compared multidose crystalloid hyperkalemic cardioplegic solutions with and without added red cells in 24 canine hearts subjected to 5 hr of arrest at 10 degrees c. all cardioplegic solutions were fully oxygenated at 4 degrees c before delivery. since blood cardioplegia contained ca++ carried over with the red cells, ca++ was added to the crystalloid solution in one group. the table below shows the hematocrit (hct) and ionized ca++ concentrations of the cardioplegic solutions, and coronary ar ... | 1985 | 4028363 |
| stable oil-in-water emulsions: preparation and use as vaccine vehicles for lipophilic adjuvants. | many of the most potent immunoadjuvants for inclusion in vaccines are extremely hydrophobic surfactants. lipophilic vehicles are needed as carriers for these water-insoluble adjuvants and to provide the hydrophilic-hydrophobic interfacial surface at which they act. we used emulsifiers comprised of fatty acid esters of polyoxyethylene sorbitan (tweens) or sorbitan (spans) to prepare oil-in-water (o/w) emulsions of hexadecane. emulsion stability could be predicted from a ratio of spectrophotometri ... | 1985 | 4036272 |
| role of uricase in the triggering of germination of bacillus fastidiosus spores. | the likelihood that uric acid was the only compound capable of triggering germination of bacillus fastidiosus spores was reinforced by the finding that ureidoglycollic acid, urea, nh4cl, 2,8-dihydroxypurine and a combination of l-alanine and o-carbamoyl-d-serine were ineffective as germinants. uric acid-triggered germination of b. fastidiosus was prevented by a range of inhibitors that also inhibited uricase activity in dormant spore extracts. o2 uptake during germination started immediately aft ... | 1985 | 4038258 |
| site of interaction of serotonin and desglycinamide-arginine-vasopressin in maintenance of ethanol tolerance. | rats trained to walk in a moving belt apparatus were subjected to a partial (fornix-fimbria (ff] or total (fornix-fimbria + cingulum bundles (ff + cb] chemical denervation of the dorsal serotonergic afferent pathways to the hippocampus. after chronic alcohol treatment that resulted in tolerance development to the motor-impairing effects of ethanol, desglycinamide-arginine8-vasopressin (dgavp) or saline treatment was started and the residual tolerance measured at several intervals after ethanol w ... | 1985 | 4038620 |
| spatial distribution of retinol-binding protein and retinyl palmitate hydrolase activity in normal and vitamin a-deficient rat liver. | a study was conducted to explore the spatial distribution within rat liver of two proteins importantly involved in retinoid metabolism in liver, namely, retinol-binding protein (rbp) and the enzyme retinyl palmitate hydrolase (rph). the study was conducted with both vitamin a-sufficient (control) and vitamin a-deficient rats. livers were carefully and reproducibly dissected into 11 sections each, and rbp levels and rph activities were measured for each section homogenate. both rbp and rph activi ... | 1985 | 4040159 |
| sow (sus scrofa) follicular fluid: prostaglandin content and effect on the motility of isolated oviducts. | the present study provides information regarding the effects of the sow follicular fluid (ff) on the motility of isolated segments of swine and rabbit oviducts. in addition, the concentration of prostaglandins (pgs) f2 alpha, e2 and e1 in the follicular fluid of sow ovaries isolated at different stages of the sex cycle as well as the generation of the same pgs by walls of ovarian follicles in early and late proestrus, in estrus, in metestrus and in diestrus, were explored. the stimulatory contra ... | 1985 | 4040260 |
| local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males. | the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ... | 1985 | 4040921 |
| periosteal stress-induced reactions resembling stress fractures. a radiologic and histologic study in dogs. | an external callus is always associated with so-called stress fractures, but a fracture line cannot always be demonstrated by radiologic means. in such instances it is assumed that an undisplaced fracture or microfracture must nevertheless exist for an external callus to form. in this experimental study, 18 beagles were immobilized in a shoulder spica for periods of time ranging from 6 to 32 weeks and then remobilized. at the time of sacrifice, which varied from four to 28 weeks after remobiliza ... | 1985 | 4042491 |
| cryptococcal meningitis: presentation as sudden deafness. | a patient presented with a bilateral profound hearing loss of sudden onset following a two-month neurologic illness. microscopy and culture of cerebrospinal fluid revealed cryptococcus neoformans. treatment with amphotericin b and 5-fluorocytosine failed to restore hearing. auditory brain stem response and electrical promontory stimulation suggest a profound deafness with poor neuronal survival. this is consistent with previous temporal bone histopathology reports in individuals dying of cryptoc ... | 1985 | 4050983 |
| laminar origin of direct projection from cortex area v1 to v4 in the rhesus monkey. | direct projection from cortex area v1 to v4 in the rhesus monkey was demonstrated by means of retrograde axonal transport of wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp). this projection originated at the layers 2 and 3 of the only representation area of central visual field (0-6 degrees) in v1 and terminated at the central representation of v4, whereas there was no projection between the extracentral representations of v1 and v4. correlation of the present finding with the ... | 1985 | 4052788 |
| drastic improvement in the rectal absorption profile of morphine in man. | rectal absorption of morphine hcl from aqueous vehicles at different phs in man has been compared with an orally administered solution. plasma concentrations of morphine were measured by electrochemical hplc analysis after a single dose of 10 mg morphine hcl, in a cross-over study in 7 volunteers. rectal absorption of morphine was dependent on ph, which could be explained as being due to ph partitioning. the absorption rate and bioavailability could be greatly improved, as compared to orally adm ... | 1985 | 4054201 |
| [reactions of hypothalamic neurons to stimulation of the pelvic and sciatic nerves]. | unit activity of posterior, tuberal and anterior parts of the hypothalamus was studied during stimulation of the pelvic nerve in anesthetized and immobilized cats. comparative analysis showed that neurons of the posterior hypothalamus were more responsive (89.6%) and had a shorter mean latency (33.3 +/- 2.4 msec) than neurons of more rostral parts of the hypothalamus. the focus of maximal pelvic-induced unit activity seems to be located in the posterior hypothalamus. the unit responses of the po ... | 1985 | 4054386 |
| [dna synthesis in cultures of atrial and ventricular cardiomyocytes in the rat]. | by means of 3h-thymidine autoradiography dna replicative activity has been studied in cultured atrial and ventricular myocytes, and non-muscle cells from hearts of 2-week-old rats (age when cell proliferation in the myocardium is already significantly depressed). pas-reaction was used as a cytochemical marker of cardiomyocytes: atrial myocytes are richer in glycogen than ventricular cells. labeling indices of atrial myocytes after a 24 hour exposure to 3h-thymidine were higher than ventricular o ... | 1985 | 4060232 |
| age-associated alterations in hepatic glutathione-s-transferase activities. | age-associated alterations of hepatic cytosolic glutathione-s-transferase (gst) activities towards sulfobromophthalein sodium tetrahydrate (bsp), styrene oxide (stox), trans-4-phenyl-3-butene-2-one (pbo), 1,2-dichloro-4-nitrobenzene (dcnb), and 1-chloro-2,4-dinitrobenzene (cdnb) were investigated in fischer-344 rats of both sexes with ages ranging from 1.5 to 28 months. the gst activities towards pbo and dcnb in male rats increased with age till 6-12 months when maximum values were attained, and ... | 1985 | 4062964 |
| effect of detergent on protein structure. action of detergents on secondary and oligomeric structures of band 3 from bovine erythrocyte membranes. | with special interest in the mode of action of zwitterionic detergents on proteins, a variety of detergents were examined for their ability to disrupt the secondary and quaternary structures of an anion transport protein, band 3, and its cytoplasmic 38 kda fragment from bovine erythrocyte membranes and for their effect on the binding of an anion transport inhibitor to band 3. nonionic detergents and chaps also acted as a nondenaturant in these instances, as well accepted for other proteins. thou ... | 1985 | 4063373 |
| convergence of influences from hypothalamus and locus coeruleus upon the olfactory tubercle. | the interaction of locus coeruleus (lc) and lateral hypothalamus (lh) stimulation upon the evoked potentials and unitary activity in the olfactory tubercle (ot) were studied. the difference in latency of the evoked potentials in the ot by lc or by lh stimulation seems to indicate that thin fibers compose the lc-ot pathway and that thick fibers compose the lh-ot pathway. the most common pattern of ot neuronal discharge was in bursts. the train stimulation of the lc decreased the ot neuronal disch ... | 1985 | 4063550 |
| an epidemic of acute diarrhoea in rural southern india associated with echovirus type 11 infection. | an epidemic of diarrhoea with two distinct waves affected a village of 1375 people in southern india in 1983. the first wave of the epidemic, from the last week of december 1982, had a sharp peak in january 1983 and was over by march. echovirus type 11 was isolated from patients, who also had a serum antibody response to the virus. during the second wave of the epidemic, from may to september 1983, the clinical features were different and shigella flexneri was isolated without significant viral ... | 1985 | 4067300 |
| fluorometric elisa method for rapid screening of anti-estrogen receptor antibody production in hybridoma cultures. | a micro-fluorometric elisa which required only 50 microliter of reagent in each step is reported here for rapid detection of anti-estrogen receptor (er) antibody production by hybridoma cultures. in this study, several factors which influence the sensitivity of elisa, e.g., incubation time, substrates, microtiter plates, concentration of coating antigens, choice and concentration of specific antibody and enzyme were evaluated. we observed that micro-fluorometric elisa using 4-methylumbelliferyl ... | 1985 | 4067317 |
| ledantec virus: identification as a rhabdovirus associated with human infection and formation of a new serogroup. | ledantec virus, originally recovered in 1965 from a patient with a febrile illness in senegal, was observed by thin section electron microscopy to be bullet-shaped, representative of members of the rhabdovirus group, with mean dimensions of 164 x 78 nm. particles were observed budding from the plasma membrane and moderate numbers accumulated in the intercellular spaces. in three mammalian cell lines, ledantec virus was rapidly cytopathic and replicated to moderately high titres. in complement fi ... | 1985 | 4067583 |
| classically conditioned hyperglycemia in the obese mouse. | the obese (c57bl/6j ob/ob) mouse is a commonly used animal model of non-insulin-dependent diabetes mellitus. it has recently been demonstrated that this mouse is not consistently hyperglycemic, however, unless it is subjected to environmental stress. in the present study, hyperglycemia in obese mice was induced by classical conditioning. obese diabetic mice and lean control animals were exposed to shaking stress. all animals developed hyperglycemia in response to shaking. to demonstrate classica ... | 1985 | 4070525 |
| [experience in the treatment of syphilis with penicillin combined with splenin]. | | 1985 | 4072396 |
| [immune response in splenectomized children to anti-pneumococcal vaccination]. | the antibody response following anti-pneumococcal immunization with a 14-valent vaccine was studied in 54 children, aged 2 to 18 years after splenectomy. splenectomy had been performed for traumatic, hematologic or immunologic reasons. antibody titration with respect to 12 serotypes was performed by radioimmunoassay immediately before and one month after immunization. changes in responses were studied according to the indication for splenectomy and to the vaccine serotype. the main conclusions o ... | 1985 | 4074086 |