| modulation of pps10 host range by plasmid-encoded repa initiator protein. | we report here the isolation and analysis of novel repa host range mutants of pps10, a plasmid originally found in pseudomonas savastanoi. upon hydroxylamine treatment, five plasmid mutants were selected for their establishment in escherichia coli at 37 degrees c, a temperature at which the wild-type form cannot be established. the mutations were located in different functional regions of the plasmid repa initiation protein, and the mutants differ in their stable maintenance, copy number, and ab ... | 2003 | 12562807 |
| the novel method for isolating chitinolytic bacteria and its application in screening for hyperchitinase producing mutant of alcaligenes xylosoxydans. | to develop a novel, rapid and effective screening method for chitinase producing bacteria. | 2003 | 12581369 |
| microbial community and physicochemical analysis of an industrial waste gas biofilter and design of 16s rrna-targeting oligonucleotide probes. | a study was conducted to investigate the microbial community structure, the physicochemical properties, and the relationships between these parameters of a full-scale industrial biofilter used for waste gas abatement in an animal-rendering plant. fluorescence in situ hybridization (fish) was successfully combined with digital image analysis to study the composition of the microbial community. several new nucleic acid probes were designed and established based on published 16s rdna sequences and ... | 2003 | 12588298 |
| comparison of aspartate transcarbamoylase regulation in pseudomonas alcaligenes and pseudomonas mendocina. | the regulation of aspartate transcarbamoylase activity in cell extracts of pseudomonas alcaligenes atcc 14909 and pseudomonas mendocina atcc 25411 was compared. under saturating substrate concentrations, pyrophosphate, ctp, udp and adp were highly inhibitory of the p. alcaligenes transcarbamoylase activity while pyrophosphate, udp, adp, atp and gtp were the most effective inhibitors of the p. mendocina transcarbamoylase. by examining transcarbamoylase inhibition by ribonucleotide triphosphates, ... | 2003 | 12596245 |
| distinguishing features of 16s rdna gene for five dominating bacterial genus observed in bioremediation. | defining a microbial community and identifying bacteria, at least at the genus level, is a first step in predicting the behavior of a microbial community in bioremediation. in biological treatment systems, the most dominating groups observed are pseudomonas, moraxella, acinetobactor, burkholderia, and alcaligenes. our interest lies in identifying the distinguishing features of these bacterial groups based on their 16s rdna sequence data, which could be used further for generating genus-specific ... | 2002 | 12614549 |
| crystal structure of a no-forming nitrite reductase mutant: an analog of a transition state in enzymatic reaction. | i257e was obtained by site directed mutagenesis of nitrite reductase from achromobacter cycloclastes. the mutant has no enzyme activity. its crystal structure determined at 1.65a resolution shows that the side-chain carboxyl group of the mutated residue, glu257, coordinates with the type 2 copper in the mutant and blocks the contact between the type 2 copper and its solvent channel, indicating that the accessibility of the type 2 copper is essential for maintaining the activity of nitrite reduct ... | 2003 | 12615072 |
| respiration of 13c-labeled substrates added to soil in the field and subsequent 16s rrna gene analysis of 13c-labeled soil dna. | our goal was to develop a field soil biodegradation assay using (13)c-labeled compounds and identify the active microorganisms by analyzing 16s rrna genes in soil-derived (13)c-labeled dna. our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soil associated with sampling and laboratory incubation. the new field-based assay involved the release of (13)c-labeled compounds (glucose, phenol, caffeine, and naphthalene) to soil ... | 2003 | 12620850 |
| bacteriological analysis of blood culture isolates from neonates in a tertiary care hospital in india. | this study was undertaken to determine the profile and antibiotic sensitivity patterns of aerobic isolates from blood cultures of neonates in a tertiary care hospital in new delhi, india. all blood culture reports (n = 1,828), obtained during august 1995-september 1996 from newborns admitted to the department of pediatrics and the neonatal intensive care unit at the university college of medical sciences and gtb hospital, delhi, were analyzed, and the sensitivity patterns were recorded. the posi ... | 2002 | 12659415 |
| characterization and function of met150gln mutant of copper-containing nitrite reductase from achromobacter cycloclastes iam1013. | the mutant (m150q-nir) replacing the met150 ligand of the type 1 cu center in achromobacter cycloclastes nitrite reductase (acnir) with gln has been physicochemically and functionally characterized. the electronic absorption and cd spectra of m150q-nir are similar to those of mavicyanin and stellacyanin having the 2his, cys, and gln ligands, but the epr signal has an axial character, although their blue copper proteins show rhombic epr signals. the mutant has about 80% catalytic activity of acni ... | 2003 | 12659849 |
| reconstitution of the type-1 active site of the h145g/a variants of nitrite reductase by ligand insertion. | variants of the copper-containing nitrite reductase (nir) of alcaligenes faecalis s6 were constructed by site-directed mutagenesis, by which the c-terminal histidine ligand (his145) of the cu in the type-1 site was replaced by an alanine or a glycine. the type-1 sites in the nir variants as isolated, are in the reduced form, but can be oxidized in the presence of external ligands, like (substituted) imidazoles and chloride. the reduction potential of the type-1 site of nir-h145a reconstituted wi ... | 2003 | 12680761 |
| comparison of ribotyping and restriction enzyme analysis for inter- and intraspecies discrimination of bordetella avium and bordetella hinzii. | bordetella avium is an avian respiratory disease pathogen responsible for substantial economic losses to the turkey industry. the inability to distinguish isolates has hampered outbreak investigations and prevents a complete understanding of transmission mechanisms. isolates of bordetella hinzii, often referred to as b. avium-like or as alcaligenes faecalis type ii prior to 1995, have also been acquired from the respiratory tracts of diseased poultry but are not believed to be pathogenic for bir ... | 2003 | 12682138 |
| to the editor: alcaligenes as a pathogen in airways chronic infection in cystic fibrosis. | | 2003 | 12687601 |
| atomic resolution structures of native copper nitrite reductase from alcaligenes xylosoxidans and the active site mutant asp92glu. | we provide the first atomic resolution (<1.20 a) structure of a copper protein, nitrite reductase, and of a mutant of the catalytically important asp92 residue (d92e). the atomic resolution where carbon-carbon bonds of the peptide become clearly resolved, remains a key goal of structural analysis. despite much effort and technological progress, still very few structures are known at such resolution. for example, in the protein data bank (pdb) there are some 200 structures of copper proteins but ... | 2003 | 12691751 |
| comparison of sodium hypochlorite-based foam and peroxyacetic acid-based fog sanitizing procedures in a salmon smokehouse: survival of the general microflora and listeria monocytogenes. | the effects of fog sanitization with peroxyacetic acid (hydrogen peroxide, peracetic acid, and acetic acid in combination) on general hygiene (aerobic plate count) and on listeria monocytogenes were assessed in a slicing area at a salmon smokehouse and compared with the effects of foam sanitization with sodium hypochlorite (routinely performed at the smokehouse). two hundred twenty-three environmental samples were collected with sponges and swabs after each of the sanitization procedures, and 68 ... | 2003 | 12696681 |
| extraction of exopolymers from biofilms: the protective effect of glutaraldehyde. | the extraction of the exopolymeric matrix is a prerequisite to properly assessing the composition of the biofilm. several extraction methods have already been developed, however, no universal method has yet been adopted because the compromise between high yields of extraction and minimum cell lysis is difficult to establish. in fact, most of the extraction methods promote leakage of intracellular material. the most common extraction methods, dowex resin and sonication, were assayed in biofilms o ... | 2003 | 12701925 |
| loop-contraction mutagenesis of a type 1 copper site. | loop-contraction mutagenesis has been applied to the cupredoxin pseudoazurin to introduce the active-site loop of amicyanin. the mutation has a limited effect on the spectroscopic properties, and therefore structure, of the cupric protein. the loop contraction results in the increase of the pka for the detachable his ligand of pseudoazurin by two ph units, similar to the value as found in amicyanin. | 2003 | 12708836 |
| analysis of a bioactive beta-(1 --> 3) polysaccharide (curdlan) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | this paper focuses on the development of maldi sample preparation protocols for the analysis of a bioactive beta-(1 --> 3) polysaccharide, i.e. curdlan. the crude curdlan sample was first separated into a low molecular weight water-soluble portion and a high molecular weight water-insoluble portion. the water-soluble portion was analyzed using a standard maldi sample preparation method developed for dextran analysis. two low-mass (<4000 da) polysaccharide distributions differing by 16 da were ob ... | 2003 | 12717760 |
| conjugative plasmid mediated inducible nickel resistance in hafnia alvei 5-5. | hafnia alvei 5-5, isolated from a soil-litter mixture underneath the canopy of the nickel-hyperaccumulating tree sebertia acuminata (sapotaceae) in new caledonia, was found to be resistant to 30 mm ni(2+) or 2 mm co(2+). the 70-kb plasmid, pejh 501, was transferred by conjugation to escherichia coli, serratia marcescens, and klebsiella oxytoca. transconjugant strains expressed inducible nickel resistance to between 5 and 17 mm ni(2+), and cobalt resistance to 2 mm co(2+). a 4.8-kb sal- ecori fra ... | 2003 | 12730713 |
| evaluation of spectrofluorometry as a tool for estimation in fed-batch fermentations. | native culture fluorescence was investigated as an additional source of information for predicting biomass and glucose concentrations in a fed-batch fermentation of alcaligenes eutrophus. partial least squares (pls) regression and a feed forward neural network (ffnn) coupled with principle component analysis (pca) were each used to model the kinetics of the fermentation. data from three fermentations was combined to form a training set for model calibration and data from a fourth fermentation wa ... | 2003 | 12740937 |
| correlation between structure of the lactones and substrate specificity in enzyme-catalyzed polymerization for the synthesis of polyesters. | small-size (4-membered) and medium-size (5-, 6-, and 7-membered) unsubstituted lactones as well as unsubstituted macrolides (12 and 13 membered) were subjected to the ring-opening polymerization using the extracellular phb depolymerase from alcaligenes faecalis t1 (phaz(afa)). the characteristic reactivities of the lactones were discussed based on a tertiary structure model of the active site of the phaz(afa). with respect to the ring-size of the lactones, the 4-membered beta-propiolactone and 6 ... | 2003 | 12741767 |
| the treatment of gaseous benzene by two-phase partitioning bioreactors: a high performance alternative to the use of biofilters. | a 2-l (1-l working volume) two-phase partitioning bioreactor (tppb) was used as an integrated scrubber/bioreactor in which the removal and destruction of benzene from a gas stream was achieved by the reactor's organic/aqueous liquid contents. the organic solvent used to trap benzene was n-hexadecane, and degradation of benzene was achieved in the aqueous phase using the bacterium alcaligenes xylosoxidans y234. a gas stream with a benzene concentration of 340 mg l(-1) at a flow rate of 0.414 l h( ... | 2003 | 12743750 |
| achromobacter xylosoxidans bacteremia: a 10-year analysis of 54 cases. | fifty-four cases of achromobacter xylosoxidans bacteremia diagnosed over a 10-year period in patients from 2 months to 87 years of age were reviewed. fifty-two episodes were nosocomial. the most frequent underlying condition was neoplasm (solid or hematological). the source of infection was a contaminated intravenous catheter in 35 patients (60%) and pneumonia in 6 patients. eight (15%) patients died. the only risk factors significantly associated with mortality were age over 65 years and neutro ... | 2003 | 12750959 |
| long-term analysis of diesel fuel consumption in a co-culture of acinetobacter venetianus, pseudomonas putida and alcaligenes faecalis. | the dynamics of a microbial population isolated from superficial waters of venice lagoon and the ability to utilise diesel fuel (n-alkanes mixture c12-c28) as the sole carbon and energy source were studied in a long-term reconstruction experiment. the reconstructed microbial population consisted of three bacterial strains belonging to the species acinetobacter venetianus, pseudomonas putida, and alcaligenes faecalis, which were able to oxidise n-alkanes to alkanoates, n-alkanols to alkanoates, o ... | 2003 | 12755474 |
| modulation of virulence by two acidified nitrite-responsive loci of salmonella enterica serovar typhimurium. | two acidified nitrite-inducible genes of salmonella enterica serovar typhimurium were identified with a green fluorescent protein-based promoter-trap screen. the nitrite-inducible promoters were located upstream of loci that we designated nipab and nipc, which correspond to hcp-hcr (hybrid cluster protein) of escherichia coli and nora of alcaligenes eutrophus, respectively. maximal induction of the promoters by nitrite was dependent on ph. the nipab promoter was regulated by oxygen in an fnr-dep ... | 2003 | 12761099 |
| infection control recommendations for patients with cystic fibrosis: microbiology, important pathogens, and infection control practices to prevent patient-to-patient transmission. | | 2003 | 12762292 |
| purification and characterization of alcaligenes faecalis penicillin g acylase expressed in bacillus subtilis. | the alcaligenes faecalis pga gene encoding heterodimeric penicillin g acylase (pga) was cloned and successfully expressed in escherichia coli and bacillus subtilis respectively. in contrast to e.coli hosts where the enzymes were retained in the periplasm, b. subtilis cell secreted the recombinant enzyme into the medium. contrary to limited expression yield of e. coli (petapga), pga extracellularly expressed by b. subtilis (pbapga) and b. subtilis (pmapga) reached the highest yield of 653 u/l. th ... | 2003 | 12766801 |
| microbial keratitis and vision loss with contact lenses. | microbial keratitis is the only sight-threatening adverse event that occurs with contact lens wear. this article gives a preliminary estimation of the incidence of microbial keratitis and vision loss with continuous-wear contact lenses made from highly oxygen permeable (dk) materials. | 2003 | 12772749 |
| a two-faced molecule offers no explanation: the proximal binding of nitric oxide to haem. | cytochrome c ' (cyt c ') is found in the periplasmic space of denitrifying bacteria where it is thought to mediate the transfer of no between the nitrogen-cycle enzymes dissimilatory nitrite reductase and nitric oxide reductase. it contains a 5-coordinate (5c) his-ligated haem that shares spectroscopic and ligand-binding properties with the haem group in the sensory domain of soluble guanylate cyclase (sgc). the latter is an extremely important enzyme involved in the control of vasodilation and ... | 2003 | 12773155 |
| the structure of glyceraldehyde 3-phosphate dehydrogenase from alcaligenes xylosoxidans at 1.7 a resolution. | the enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh) from the gram-negative denitrifying bacterial species alcaligenes xylosoxidans was purified and crystallized as a contaminant protein during purification of nitrous oxide reductase. this is the first structure of a gapdh from a denitrifying species. the crystal structure was solved at 1.7 a resolution by molecular replacement using the structure of gapdh from bacillus stearothermophilus as a starting model. the quality of the structure ... | 2003 | 12777799 |
| introduction of a pi-pi interaction at the active site of a cupredoxin: characterization of the met16phe pseudoazurin mutant. | the met16phe mutant of the type 1 copper protein pseudoazurin (pacu), in which a phenyl ring is introduced close to the imidazole moiety of the his81 ligand, has been characterized. nmr studies indicate that the introduced phenyl ring is parallel to the imidazole group of his81. the mutation has a subtle effect on the position of the two s(cys)-->cu(ii) ligand-to-metal charge transfer bands in the visible spectrum of pacu(ii) and a more significant influence on their intensities resulting in a a ... | 2003 | 12779340 |
| chemoenzymatic synthesis of (e)-3,7-dimethyl-2-octene-1,8-diol isolated from the hairpencils of male danaus chrysippus (african monarch). | the synthesis of (e)-3,7-dimethyl-2-octene-1,8-diol (1), which was isolated from the hairpencils of male danaus chrysippus (african monarch), was investigated. the key step of the sequence involves asymmetric desymmetrization of the 1,3-propanediol 7 with lipase, in which high enantioselectivity was observed. total synthesis afforded (s)-1 in 12 steps and 26% overall yield from readily available geraniol. | 2003 | 12781175 |
| molecular diversity of denitrifying genes in continental margin sediments within the oxygen-deficient zone off the pacific coast of mexico. | to understand the composition and structure of denitrifying communities in the oxygen-deficient zone off the pacific coast of mexico, the molecular diversity of nir genes from sediments obtained at four stations was examined by using a pcr-based cloning approach. a total of 50 operational taxonomic units (otus) for nirk and 82 otus for nirs were obtained from all samples. forty-four of the nirs clones and 31 of the nirk clones were sequenced; the levels of similarity of the nirs clones were 52 t ... | 2003 | 12788762 |
| ambient stm and in situ afm study of nitrite reductase proteins adsorbed on gold and graphite: influence of the substrate on protein interactions. | trimeric achromobacter cycloclastes cu-containing nitrite reductase (cunir) proteins adsorbed on gold and graphite have been studied by ambient stm and in situ afm. stm resolves them individually and in layers, distinguishing the sub-molecular individual units of the trimer. the cu atoms are not visible to stm. stm shows that individual cunir denatures as it adsorbs on au, although a deformed trimeric shape can be identified in some cases. cunir forms disordered layers on gold. on graphite, orde ... | 2003 | 12801658 |
| development of a gram-negative selective agar (gnsa) for the detection of gram-negative microflora in sputa in patients with cystic fibrosis. | to develop a selective agar medium to help detect and quantify gram-negative flora in the sputum of patients with cystic fibrosis (cf). | 2003 | 12807467 |
| [changes of antimicrobial resistance among nonfermenting gram-negative bacilli isolated from intensive care units from 1994 to 2001 in china]. | to investigate the antimicrobial resistance among nonfermenting gram-negative bacilli isolated from intensive care units (icus) during the past 7 years in china. | 2003 | 12820914 |
| ralstonia metallidurans, a bacterium specifically adapted to toxic metals: towards a catalogue of metal-responsive genes. | ralstonia metallidurans, formerly known as alcaligenes eutrophus and thereafter as ralstonia eutropha, is a beta-proteobacterium colonizing industrial sediments, soils or wastes with a high content of heavy metals. the type strain ch34 carries two large plasmids (pmol28 and pmol30) bearing a variety of genes for metal resistance. a chronological overview describes the progress made in the knowledge of the plasmid-borne metal resistance mechanisms, the genetics of r. metallidurans ch34 and its ta ... | 2003 | 12829276 |
| a novel enzyme, d-3-hydroxyaspartate aldolase from paracoccus denitrificans ifo 13301: purification, characterization, and gene cloning. | a novel enzyme, d-3-hydroxyaspartate aldolase (d-haa), catalyzing the conversion of d-3-hydroxyaspartate to glyoxylate plus glycine, was purified to homogeneity from paracoccus denitrificans ifo 13301. d-haa is strictly d-specific as to the alpha-position, whereas the enzyme does not distinguish between threo and erythro forms at the beta-position. in addition to d-3-hydroxyaspartate, the enzyme also acts on d-threonine, d-3-3,4-dihydroxyphenylserine, d-3-3,4-methylenedioxyphenylserine, and d-3- ... | 2003 | 12835921 |
| quinolinate dehydrogenase and 6-hydroxyquinolinate decarboxylase involved in the conversion of quinolinic acid to 6-hydroxypicolinic acid by alcaligenes sp. strain uk21. | in the conversion of quinolinic acid to 6-hydroxypicolinic acid by whole cells of alcaligenes sp. strain uk21, the enzyme reactions involved in the hydroxylation and decarboxylation of quinolinic acid were examined. quinolinate dehydrogenase, which catalyzes the first step, the hydroxylation of quinolinic acid, was solubilized from a membrane fraction, partially purified, and characterized. the enzyme catalyzed the incorporation of oxygen atoms of h(2)o into the hydroxyl group. the dehydrogenase ... | 2003 | 12844210 |
| [bacteraemia in patients with hiv disease: aetiological spectrum and profile of antimicrobial susceptibility]. | a retrospective survey of non-opportunistic bacterial pathogens isolated from blood cultures of patients with hiv disease has been carried out for a 6-year period, and the antibiotic susceptibility of the 748 microorganisms cultured from 682 consecutive patients, has been evaluated. gram-positive organisms significantly prevailed over gram-negative ones, with staphylococcus aureus, coagulase-negative staphylococci, enterococcus faecalis, xantomonas maltophilia, salmonella and pseudomonas sp. as ... | 1997 | 12847308 |
| a homology model of penicillin acylase from alcaligenes faecalis and in silico evaluation of its selectivity. | a three-dimensional model of the relatively unknown penicillin acylase from alcaligenes faecalis (pa-af) was built up by means of homology modeling based on three different crystal structures of penicillin acylase from various sources. an in silico selectivity study was performed to compare this homology model to the structure of the escherichia coli enzyme (pa-ec) in order to find any selectivity differences between the two enzymes. the program grid was applied in combination with the principal ... | 2003 | 12851931 |
| production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with high molar fractions of 3-hydroxyvalerate by a threonine-overproducing mutant of alcaligenes sp. sh-69. | a threonine overproducing mutant of alcaligenes sp. sh-69 was isolated and its ability to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3hb-co-3hv), was investigated. the 3hv fraction in poly(3hb-co-3hv) produced from glucose as the sole carbon source exceeded 22 mol%, which is approximately six times higher than that achieved by the wild type under the same culture conditions. furthermore, the addition of a relatively low concentration (10 mm) of propionic acid, valeric acid or lev ... | 2003 | 12882163 |
| purification and characterization of chitinase from alcaligenes xylosoxydans. | extracellular chitinase from alcaligenes xylosoxydans was purified to electrophoretic homogeneity using affinity and gel filtration chromatography. the molecular mass of chitinase was estimated to be 45 kda and 44 kda by sds-page and gel-filtration, respectively. the enzyme was optimally active at 50 degrees c (over 30 min) and ph 5. activity staining after page showed a single band. the km for chitin was 3 g l-1. cu2+ and na+ at 5 mm inhibited chitinase activity to 25% while ca2+, mg2+ and ba2+ ... | 2003 | 12882172 |
| chemical modification of lipases with various hydrophobic groups improves their enantioselectivity in hydrolytic reactions. | semi-purified lipases from candida rugosa, pseudomonas cepacia and alcaligenes sp. were chemically modified with a wide range of hydrophobic groups such as benzyloxycarbonyl, p-nitrobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl, t-butoxycarbonyl, lauroyl and acetyl moieties. the candida rugosa lipase my modified with the benzyloxycarbonyl group (modification ratio = 84%) brought about a 15-fold increase in enantioselectivity (e value) towards the hydrolysis of racemic butyl 2-(4-ethylphenoxy)pro ... | 2003 | 12882312 |
| bioreactor cultivation of escherichia coli for production of recombinant penicillin g amidase from alcaligenes faecalis. | the penicillin g amidase (pga) from alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in escherichia coli. the corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter. cells were grown in a synthetic minimal medium in a bioreactor (5 l working vol.), and production of pga was induced by repeated addition of the inducer rhamnose, that served also ... | 2003 | 12882560 |
| control of metalloprotein reduction potential: compensation phenomena in the reduction thermodynamics of blue copper proteins. | the reduction thermodynamics (delta h degrees '(rc) and delta s degrees '(rc)) for native paracoccus versutus amicyanin, for alcaligenes faecalis s-6 pseudoazurin, and for the g45p, m64e, and k27c variants of pseudomonas aeruginosa azurin were measured electrochemically. comparison with the data available for other native and mutated blue copper proteins indicates that the features of metal coordination and the electrostatic potential due to the protein matrix and the solvent control the reducti ... | 2003 | 12885256 |
| in silico prediction and validation of the importance of the entner-doudoroff pathway in poly(3-hydroxybutyrate) production by metabolically engineered escherichia coli. | the metabolic network of escherichia coli was constructed and was used to simulate the distribution of metabolic fluxes in wild-type e. coli and recombinant e. coli producing poly(3-hydroxybutyrate) [p(3hb)]. the flux of acetyl-coa into the tricarboxylic acid (tca) cycle, which competes with the p(3hb) biosynthesis pathway, decreased significantly during p(3hb) production. it was notable to find from in silico analysis that the entner-doudoroff (ed) pathway flux increased significantly under p(3 ... | 2003 | 12889025 |
| a study on microbial diversity in different cultivars of brassica napus in relation to its wilt pathogen, verticillium longisporum. | oilseed rape (brassica napus) is one of the major oilseed crops in the world but is vulnerable to attack by many pathogens and insect pests. in addition to the host plant genotype, micro-organisms present in the rhizosphere and within plant tissues affect the susceptibility to plant pathogens. while rapid progress has been achieved concerning the concept of plant resistance genes, information on the role of the microbial community in plant protection is less apparent. we have studied the endophy ... | 2003 | 12892892 |
| a novel kinetic trap for no release from cytochrome c': a possible mechanism for no release from activated soluble guanylate cyclase. | flash photolysis studies on the five-coordinate heme nitrosyl of alcaligenes xylosoxidans cytochrome c' were carried out to investigate the ramifications of its proximal nitrosyl ligand on no release. delta absorbance spectra recorded 5 ms after photolysis indicate that approximately 5% of the photolyzed hemes are converted to a five-coordinate high spin ferrous state, revealing that reattachment of the endogenous his ligand is fast enough to trap some of the photolyzed heme. analysis of no rebi ... | 2003 | 12903995 |
| nitrogen fixation genetics and regulation in a pseudomonas stutzeri strain associated with rice. | the pseudomonas stutzeri strain a1501 (formerly known as alcaligenes faecalis) fixes nitrogen under microaerobic conditions in the free-living state and colonizes rice endophytically. the authors characterized a region in strain a1501, corresponding to most of the nif genes and the rnf genes, involved in electron transport to nitrogenase in rhodobacter capsulatus. the region contained three groups of genes arranged in the same order as in azotobacter vinelandii: (1) nifb fdx orf3 nifq orf5 orf6; ... | 2003 | 12904565 |
| effect of carbon source on pyrimidine biosynthesis in pseudomonas alcaligenes atcc 14909. | the effect of carbon source on the regulation of the de novo pyrimidine biosynthetic enzymes in pseudomonas alcaligenes atcc 14909 was investigated. the de novo pyrimidine biosynthetic enzymes were measured in extracts of p. alcaligenes atcc 14909 cells and of cells from an auxotroph deficient for orotate phosphoribosyltransferase activity. pyrimidine biosynthetic enzyme activities in atcc 14909 were influenced by pyrimidine supplementation to the culture medium but not by the carbon source pres ... | 2003 | 12906394 |
| molecular characterization of an inducible gentisate 1,2-dioxygenase gene, xlne, from pseudomonas alcaligenes ncimb 9867. | pseudomonas alcaligenes ncimb 9867 (strain p25x) produces isofunctional enzymes of the gentisate pathway that enables the degradation of xylenols and cresols via gentisate. previous reports had indicated that one set of enzymes is constitutively expressed whereas the other set is strictly inducible by aromatic hydrocarbon substrates. the gene encoding gentisate 1,2-dioxygenase (gdo), the enzyme that catalyzes the cleavage of the gentisate aromatic ring, was cloned from strain p25x. the gdo gene, ... | 2003 | 12909360 |
| imipenem resistance in nonfermenters causing nosocomial urinary tract infections. | nonfermenting gram-negative bacilli (nonfermenters) have emerged as important nosocomial pathogens causing opportunistic infections in immunocompromised hosts. these organisms show high level of resistance to b-lactam agents, fluoroquinolones and aminoglycosides. imipenem is a carbapenem antibiotic, which can be very useful for treatment of infections caused by nonfermenters. eighty-five nonfermenters causing nosocomial uti were tested for mic to imipenem by agar dilution method. resistance to o ... | 2003 | 12928556 |
| [phenotypic and genotypic characteristics of non fermenting atypical strains recovered from cystic fibrosis patients]. | we used partial 16s rrna gene (16s dna) sequencing for the prospective identification of nonfermenting gram-negative bacilli recovered from patients attending our cystic fibrosis center (hôpital necker-enfants malades), which gave problematic results with conventional phenotypic tests. during 1999, we recovered 1093 isolates of nonfermenting gram-negative bacilli from 702 sputum sampled from 148 patients. forty-six of these isolates (27 patients) were not identified satisfactorily in routine lab ... | 2003 | 12948761 |
| regioselective hydroxylation of quinolinic acid, lutidinic acid and isocinchomeronic acid by resting cells of pyridine dicarboxylic acid-degrading microorganisms. | microorganisms aerobically degrading quinolinic acid, lutidinic acid or isocinchomeronic acid were isolated and the microbial regioselective hydroxylation of these pyridine dicarboxylic acids was studied. alcaligenes sp. uk21 cells converted quinolinic acid into 6-hydroxypicolinic acid, suggesting the involvement of two enzyme reactions catalyzing hydroxylation at position c6 and decarboxylation at position c3 of quinolinic acid. resting cells of alcaligenes sp. uk21 accumulated 94.9 mm 6-hydrox ... | 2003 | 12955355 |
| exposure of sink drain microcosms to triclosan: population dynamics and antimicrobial susceptibility. | recent concern that the increased use of triclosan (tcs) in consumer products may contribute to the emergence of antibiotic resistance has led us to examine the effects of tcs dosing on domestic-drain biofilm microcosms. tcs-containing domestic detergent (tcsd) markedly lowered biofouling at 50% (wt/vol) but was poorly effective at use levels. long-term microcosms were established and stabilized for 6 months before one was subjected to successive 3-month exposures to tcsd at sublethal concentrat ... | 2003 | 12957932 |
| delivery of benzene to alcaligenes xylosoxidans by solid polymers in a two-phase partitioning bioreactor. | toxic levels of benzene were decreased to sub-inhibitory levels in a bioreactor via absorption by polymer beads or cylinders (poly(ethylene-co-vinyl acetate) or poly(styrene-co-butadiene)). after inoculation with alcaligenes xylosoxidans, the remaining benzene in the aqueous phase as well as the benzene taken up by the polymers was degraded to completion. the capacity of these polymers for benzene, and benzene diffusivity within the polymers were also determined. | 2003 | 12967014 |
| acute postoperative alcaligenes xylosoxidans endophthalmitis. | | 2003 | 12972784 |
| infective endocarditis due to bact. faecalis alcaligenes. | | 1952 | 12978364 |
| [suppurative meningitis in infant caused by alcaligenes faecalis]. | | 1952 | 12987217 |
| alcaligenes faecalis meningitis complicated by hydrocephalus in a premature twin; report of case; intramuscular chloramphenicol therapy. | | 1952 | 12995997 |
| differentiation of alcaligenes faecalis from brucella bronchisepticus by biochemical and nutritional methods. | | 1953 | 13034718 |
| [an unusual case of subacute bacterial endocarditis caused by bacillus faecalis alcaligenes; combined penicillin-sulfonamide therapy]. | | 1953 | 13072050 |
| [a case of mixed meningitis caused by alcaligenes faecalis and flavobacterium arborescens]. | | 1953 | 13073497 |
| nitrogen compounds utilized by alcaligenes faecalis. | | 1953 | 13096506 |
| [a case of alcaligenes faecalis septicemia]. | | 1953 | 13101207 |
| phosphorylation coupled to electron transport in cell-free extracts of alcaligenes faecalis. | | 1953 | 13117884 |
| the nomenclatural type of the genus deleya and the consequences of deleya aesta and alcaligenes aquamarinus being synonyms. | the genus deleya was created to encompass a number of marine organisms that had previously been classified in diverse genera. deleya aesta was designated as the type species of the genus. subsequent work indicated that deleya aesta, alcaligenes aquamarinus and alcaligenes faecalis subsp. homari were heterotypic synonyms. consequently, akagawa & yamasato (int j syst bacteriol 39, 462-466, 1989) concluded that, based on rules 23a and 51b of the bacteriological code (1975 revision), the oldest legi ... | 2003 | 13130071 |
| the arylsulphatase of an alcaligenes isolated from a marine mud. | | 1953 | 13140250 |
| [comparative study of the genera moraxella, achromobacter and alcaligenes]. | | 1953 | 13148811 |
| studies on a psychrophilic bacterium causing ropiness in milk. i. morphological and physiological considerations. | | 1954 | 13149150 |
| studies on a psychrophilic bacterium causing ropiness in milk. ii. chemical nature of the capsular polysaccharide. | | 1954 | 13149151 |
| b. faecalis alkaligenes infections with a report of a case of septicaemia. | | 1954 | 13185839 |
| studies on sulphatases. viii. the arylsulphatase of a strain of alcaligenes metalcaligenes isolated from intertidal mud. | | 1954 | 13208569 |
| alcaligenes denitrificans n.sp. | | 1954 | 13221772 |
| [alcaligenes epidemic in rabbits treated with cortisone]. | | 1954 | 13225408 |
| the functional groupings of the arylsulphatase of alcaligenes metalcaligenes. | | 1955 | 13239595 |
| studies on sulphatase. ii. the purification and properties of the arylsulphatase of alcaligenes metalcaligenes. | | 1955 | 13269370 |
| [clinicobacteriological considerations and antibiotic spectrum of alcaligenes and similar bacteria]. | | 1955 | 13273765 |
| [endocarditis lenta due to bacillus faecalis alcaligenes]. | | 1955 | 13274589 |
| d-erythrose metabolism in a strain of alcaligenes faecalis. | | 1956 | 13345750 |
| [morphologic changes of bacterium faecalis alkaligenes in unfavourable environment of growth]. | | 1956 | 13353520 |
| [the so-called incomplete l-cycle]. | | 1956 | 13353526 |
| studies on sulphatases. 13. the hydrolysis of substituted phenyl sulphates by the arylsulphatase of alcaligenes metalcaligenes. | | 1956 | 13363831 |
| [case of puerperal sepsis caused by alcaligenes faecalis]. | | 1956 | 13384634 |
| [haemophilus bronchisepticus and alcaligenes faecalis. i. some differential characters]. | | 1955 | 13403279 |
| [haemophilus bronchisepticus and alcaligenes faecalis. ii. sensitivity to antibiotics]. | | 1955 | 13403280 |
| [bacillus faecalis alcaligenes and human pathology; study of 80 strains]. | | 1957 | 13422460 |
| potassium uptake by alcaligenes faecalis. | | 1957 | 13426108 |
| alcaligenes haemagglutinans: activity and stability of the hemagglutinating property. | | 1957 | 13432738 |
| [comparison of calcium fractions in the cerebrospinal fluid and in the blood serum in normal humans]. | | 1957 | 13473255 |
| degradation of ergothioneine by alcaligenes faecalis. | | 1957 | 13475253 |
| a polynucleotide coenzyme of oxidative phosphorylation. | | 1957 | 13491553 |
| a polynucleotide coenzyme of oxidative phosphorylation. ii. | | 1957 | 13491555 |
| [pyrogenic and therapeutic effect of a new bulgarian vaccine]. | | 1957 | 13506943 |
| [causes of a mucoid ring formation of alcaligenes around antibiotic containing disks]. | | 1957 | 13509669 |
| abnormal growth induced by penicillin in a strain of alcaligenes fecalis. | | 1958 | 13523458 |
| variation during the cell-division cycle in the penicillin induction of protoplast-like forms of alcaligenes fecalis. | | 1958 | 13523459 |
| [intestinal infections by alcaligenes faecalis & their analogy with intestinal syndromes due to moraxella lwoffi]. | | 1958 | 13534033 |
| alcaligenes haemagglutinans: factors determining the agglutinability of the red blood cells and the mechanism of the agglutination. | | 1958 | 13555772 |
| alcaligenes haemolysans. | | 1958 | 13566061 |