| the effects of fk-506, a novel and potent immunosuppressant, upon murine coxsackievirus b3 myocarditis. | to test the therapeutic efficacy of immunosuppression with fk-506 upon coxsackievirus b3 myocarditis, c3h/he mice were inoculated with coxsackievirus b3, and the effects of fk-506 were compared to those of cyclosporine. fk-506 (2.5 mg/kg/day) or cyclosporine (25 mg/kg/day) was administered s.c. daily on days 0 to 14 (experiment i) and on days 14 to 28 (experiment ii). in experiment i, the survival rate of the fk-506 or cyclosporine-treated group was significantly lower compared with that of the ... | 1992 | 1372051 |
| [epidemic outbreak of viral meningitis caused by type 30 echo virus]. | the clinical records of 15 children admitted to hospital infantile de méxico federico gómez with diagnosis of viral meningitis were reviewed. they were part of 19 patients admitted with this diagnosis during a 5 week period (march 22 to april 30, 1992) and represent a significant increase of aseptic meningitis compared with the same periods of previous years at hospital infantile de mexico and in mexico city where there is an ongoing epidemic outbreak of this entity. all the patients studied had ... | 1992 | 1418647 |
| disturbance of mouse pancreatic beta-cell function following echo 4 virus infection. | | 1992 | 1486985 |
| finding out how a viral hitchhiker snags a ride. | | 1992 | 1553554 |
| identification of the integrin vla-2 as a receptor for echovirus 1. | cell surface receptors for echovirus, a common human pathogen, were identified with monoclonal antibodies that protected susceptible cells from infection with echovirus 1. these monoclonal antibodies, which prevented virus attachment to specific receptor sites, recognized the alpha and beta subunits of the integrin vla-2 (alpha 2 beta 1), a receptor for collagen and laminin. rd rhabdomyosarcoma cells expressed little vla-2, did not bind to 35s-labeled virus, and resisted infection until transfec ... | 1992 | 1553561 |
| a 44,000 glycoprotein is involved in the attachment of echovirus-11 onto susceptible cells. | cellular receptors play an important role in viral pathogenesis. until now little was known on echovirus (ev) receptor. using detergent-treated kb cell extracts as immunogen, a mouse monoclonal antibody (mab 143) was produced that selectively blocks the attachment of ev-11 to kb and other susceptible cells. by immunoblotting, mab 143 detected a 44,000 protein on susceptible cell lines but not on cell lines from nonprimate origin. the receptor protein complex, purified from kb cell membranes by i ... | 1992 | 1604819 |
| replication of enteroviruses in human mononuclear cells. | | 1992 | 1607276 |
| mapping of a neutralizing antigenic site of coxsackievirus b4 by construction of an antigen chimera. | a neutralizing antigenic site of coxsackievirus b4 (cvb4) was identified by construction of an antigen chimera between coxsackievirus b3 (cvb3) and cvb4. this chimera, designated cvb3/4, was constructed by inserting five amino acids of the putative bc loop of the structural protein vp1 of cvb4 into the corresponding loop of cvb3 by site-directed mutagenesis of infectious recombinant cvb3 cdna. the chimeric cdna was capable of inducing an infectious cycle upon transfection of permissive host cell ... | 1991 | 1645779 |
| [epidemiologic factors and serum antibody titer to coxsackie b-4 virus in patients with type i diabetes]. | in 150 patients (aged 4-16) with type i diabetes, hospitalized in the paediatric institute in lublin in the years 1968 bis 1979, some epidemiological data were analysed, including age, sex and the month of onset of diabetes. an increase of the number of onsets of diabetes type i was observed in the second half of the year, particularly in september and october, similarly to the occurrence of some virological infections, including coxsackie b-4. our question was if the coxsackie b-4 virus could h ... | 1991 | 1647469 |
| lps promotes cb3-induced myocarditis in resistant b10.a mice. | coxsackie virus b3 (cb3) infection of a/j or a.sw mice results in autoimmune myocarditis characterized by a diffuse mononuclear cell infiltrate and heart-specific autoantibodies. c57bl/10 congenic mice that are identically treated are resistant to this disease. cb3-infected resistant b10.a mice were treated with lps to determine if this immunomodulator alters disease susceptibility. in contrast to mice infected only with cb3 or treated only with lps. cb3-infected/lps-treated (cb3/lps) b10.a mice ... | 1991 | 1647882 |
| limb paralysis as a manifestation of coxsackie b virus infection. | forty-four african children (25 girls, 19 boys) are described with single or multiple limb paralysis as a manifestation of infection with coxsackie b virus. single limb paralysis involved exclusively the lower extremity, with no nervous system involvement elsewhere and a relatively benign prognosis. multiple limb paralysis occurred either alone or with other neurological deficits. only two of 15 patients followed up for five months or longer regained premorbid motor status. seven patients requir ... | 1991 | 1648520 |
| susceptibility of normal human joint tissue to viruses. | a model system has been developed to investigate the comparative ability of different viruses to replicate and persist intraarticularly. the viruses chosen for study were rubella, mumps, coxsackie b4, adenovirus and varicella zoster, a selection of viruses with different degrees of association with joint symptoms in clinical studies. our results showed that these viruses demonstrated a range of abilities to infect and persist in human joint tissue cultured in vitro. the most arthritogenic viruse ... | 1991 | 1650840 |
| nucleotide sequences important for translation initiation of enterovirus rna. | an infectious cdna clone was constructed from the genome of coxsackievirus b1 strain. a number of rna transcripts that have mutations in the 5' noncoding region were synthesized in vitro from the modified cdna clones and examined for their abilities to act as mrnas in a cell-free translation system prepared from hela s3 cells. rnas that lack nucleotide sequences at positions 568 to 726 and 565 to 726 were found to be less efficient and inactive mrnas, respectively. to understand the biological s ... | 1991 | 1651409 |
| [coxsackie b virus in children with acute myocarditis]. | to study etiopathogenic and follow-up aspects of patients with myocarditis. | 1991 | 1653563 |
| genetics of coxsackie b3 (cvb3) myocarditis. | the pathogenic t cell responsible for myocarditis following cvb3 infection differs between balb/c and dba/2 mice. since these two strains are identical at the major histocompatibility (mhc) loci (both h-2d), our aim was to investigate the genetic basis for this difference in t cell immunity, as well as the tissue origin responsible. as previously described, dba/2 mice are protected from developing myocarditis by cd4+ t cell depletion; balb/c cum mice could be protected by cd8+ depletion. txbm db ... | 1991 | 1655440 |
| cardiac myosin-induced myocarditis as a model of postinfectious autoimmunity. | immunization with purified cardiac myosin induces autoimmune myocarditis in a strain mice. because this disease parallels coxsackievirus b3 (cb3)-induced myocarditis in many respects, we are now using the immunization model as a virus-free system to study certain forms of immunologically-mediated heart disease. in the present article we will describe several characteristics of the humoral and cellular autoimmune response acting in myosin-induced myocarditis. furthermore, we discuss hypotheses wh ... | 1991 | 1655442 |
| naturally occurring anti-idiotypic antibodies--mechanisms for autoimmunity and immunoregulation? | coxsackievirus b3 (cvb3) infections induce myocardial inflammation which is presumably mediated by autoimmune mechanisms in the experimental murine model. naturally occurring antibodies that also bind to myocytes have been identified that can either abrogate or enhance cardiac injury by modulating the immune response. monoclonal antibodies derived from cvb3-infected mice were immobilized to plastic and overlaid with spleen cells obtained from mice infected with 5 x 10(4) plaque forming units of ... | 1991 | 1655445 |
| effect of dexamethasone on coxsackievirus b2-infected rat beating heart cells in culture. | the effects of dexamethasone on cultured rat beating heart cells experimentally infected with coxsackievirus b2 were investigated at an early stage (1-3 days) post challenge. changes in the release of the cardiac enzyme, aspartate amino-transferase (ast), beating %, cytopathic effect (cpe), virus titre, ultrastructure and alterations of the electrical activity were evaluated. the protective effects of dexamethasone on infected cells were abolished except the release of ast at 5 days post infecti ... | 1991 | 1655450 |
| an attenuated variant of coxsackievirus b3 preferentially induces immunoregulatory t cells in vivo. | balb/c mice infected with the woodruff variant of coxsackievirus group b type 3 (cvb3w) develop myocarditis mediated by autoimmune cytolytic t lymphocytes. a variant of cvb3w (designated h3-10a1) which infects the myocardium but induces minimal mortality of myocarditis compared to the parental virus was selected. although h3-10a1 infections stimulate normal ctl responses to cvb3-infected myocytes, the autoimmune response to myocardial antigens is absent. treatment of h3-10a1-infected mice with 5 ... | 1991 | 1656071 |
| immune responsiveness in chronic fatigue syndrome. | we have endeavoured to find immunological indications of chronic virus infection in patients with chronic fatigue syndrome (myalgic encephalomyelitis) and to investigate immune responsiveness to viruses in such patients in comparison with normal subjects and patients with muscular dystrophy. levels of circulating igm immune complexes were elevated (above the 95% normal control range) in 10 (17%) of 58 patients with chronic fatigue syndrome, which was not significantly different from the normal c ... | 1991 | 1656416 |
| genetic control of coxsackievirus b3-induced heart-specific autoantibodies associated with chronic myocarditis. | cardiac-specific autoantibodies to sarcolemmal and cardiac myosin antigens observed during the chronic phase of coxsackievirus b3-induced myocarditis appear to be under autosomal recessive control. this observation is based on examination of f1 hybrids bred from a/j mice which develop chronic myocarditis and c57bl/6j mice which resolve the virus-induced lesions. previous mouse studies demonstrated that the prevalence of heart-specific autoantibodies varied with the h-2 complex. however, in 25 h- ... | 1991 | 1657464 |
| coxsackie virus b4 infection of the mouse pancreas: i. detection of virus-specific rna in the pancreas by in situ hybridisation. | the pathology of coxsackie virus b4 (cvb4) infection in a murine model was investigated by in situ hybridisation using a biotinylated cdna probe derived from cvb4. during the acute phase of infection virus rna sequences were detected in the exocrine pancreas of 60% of mice infected with a pancreotropic variant of cvb4. a positive hybridisation signal was observed in other organs in some animals including the heart and liver of 1 mouse 28 days after infection. the cellular distribution of virus r ... | 1991 | 1658221 |
| [clinical and experimental study of the effect of kang er xin-i on viral myocarditis]. | kang er xin-i (kex-i) is a proved recipe used to treat viral myocarditis. it consists of lonicera japonica, ophiopogon japonicus, astragalus membranaceus mainly and possesses the effect of clearing away heat and toxic materials and supplementing the vital energy and nourishing. the clinical study was carried out with kex-i according to a random, paired and cross-over design. coenzyme q10 was used as a control and left ventricular function was observed. the result showed: after being treated with ... | 1991 | 1659497 |
| microinjected coxsackie b1 virus does not replicate in hep-2 cells. | coxsackie b1 virus did not replicate when microinjected into hep-2 cells. replication was assayed by production of infectious virus particles, synthesis of viral rna, and lysis of cells. the same virus preparation initiated replication when it was inoculated into hep-2 cells similarly to that by microinjected purified coxsackie b1 virus rna. | 1991 | 1660207 |
| coxsackievirus b4-induced development of antibodies to 64,000-mr islet autoantigen and hyperglycemia in mice. | diabetogenic coxsackievirus b4 infection produces a diabetes syndrome in susceptible mice resembling insulin-dependent diabetes mellitus. we reported a two- to threefold increased expression of a 64,000-mr (64 k) islet autoantigen in the infected mice preceding the development of hyperglycemia, suggesting a possible role of the virus in autoimmunity. to assess if the virus could be a trigger of autoimmunity, 64 k autoantibody development was correlated with hyperglycemia and virus replication in ... | 1991 | 1660313 |
| heart-specific autoantibodies can be eluted from the hearts of coxsackievirus b3-infected mice. | this study was undertaken to determine if immunoglobulin g (igg) antibodies could be eluted from the hearts of mice with coxsackievirus b3-induced autoimmune myocarditis and to characterize the immunoreactivity of any elutable autoantibodies. susceptible (a/j) and resistant (b10.a) mice were administered the virus or the control treatment and killed at various times after treatment. acid eluates from pooled heart tissue from each treatment group and each time were tested for igg reactivity with ... | 1991 | 1660795 |
| pathogenesis of myocardial injury in myocarditis and cardiomyopathy. | the pathogenesis of myocardial cell injury in myocarditis and cardiomyopathy was investigated. the presence of viral genomes in the murine heart in experimental coxsackievirus b3 myocarditis was studied by northern blotting analysis using a 32p-labeled cdna probe from the 5' end sequence. the strongest signal of positive autoradiograms was always at about 7.4 kilobases, corresponding to the size of the complete genome of the virus. successive infections with coxsackievirus and encephalomyocardit ... | 1991 | 1660942 |
| [viral infection in the neonatal period: diagnostic difficulties, the role of interferon alpha levels]. | serum and/or cerebrospinal fluid (csf) alpha interferon (aifn) levels were determined in 31 neonates hospitalized for suspected sepsis. final diagnosis was bacterial sepsis in 15, viral infection in 13 and no infection in 3. among the 13 neonates with viral infection, enterovirus was isolated 5 times and coxsackievirus 4 times. no aifn was found in cerebro-spinal fluid and/or serum among the neonates with bacterial sepsis or without infection. by contrast, in neonates with viral infection, csf a ... | 1991 | 1662355 |
| murine acinar pancreatitis preceding necrotizing myocarditis after coxsackievirus b3 inoculation. | balb/c weanling mice were inoculated intraperitoneally with a myocarditic variant of coxsackie-virus b3, with the aim of characterizing more fully the cell damage induced in the heart as well as in other organs. during the first week postinfection (pi), all animals developed acinar pancreatitis, followed by focal myocarditis. in accordance with the increasing infectivity titers, such progressive histopathological changes correlated with local viral replication. from day 4 pi, acinar degeneration ... | 1991 | 1662707 |
| no association between islet cell antibodies and coxsackie b, mumps, rubella and cytomegalovirus antibodies in non-diabetic individuals aged 7-19 years. | viral antibodies were tested in a cohort of 44 islet-cell antibody-positive individuals age 7-19 years, and 44 of their islet cell antibody-negative age and sex-matched classmates selected from a population study of 4208 pupils who had been screened for islet cell antibodies. anti-coxsackie b1-5 igm responses were detected in 14 of 44 (32%) of the islet cell antibody-positive subjects and in 7 of 44 (16%) control subjects. this difference did not reach the level of statistical significance. none ... | 1991 | 1663052 |
| [morphological study of the myocardium of mice infected with coxsackie b1 virus during the treatment with an immunomodulating drug t-activin]. | the study was undertaken to examine myocardial morphological alterations in mice treated with t-activin in different periods of viral myocarditis. two-month balb/c mice of both sexes were used in the experiments, which were intraperitoneally given 0.2 ml coxsackie b1 virus in a titer of 10(-3)-10(-7) d50 ml. the material was taken 3, 5, 7, 9, 15, and 30 days following infection. intact mice and animals given culture fluid were applied as controls. t-activin was subcutaneously injected in a dose ... | 1991 | 1664006 |
| differential effect of coxsackie b4 virus on murine pancreatic islet preproinsulin mrna and glyceraldehyde-3-phosphate dehydrogenase mrna. | | 1991 | 1665434 |
| competition binding studies with biotinylated echovirus 11 in cytofluorimetry analysis. | competition binding studies between viruses are usually performed with radiolabelled probes. in this report, a cytofluorimetric method using biotinylated echovirus (ev) 11 is described for the study of competition of enteroviruses for a common cell receptor site. an n-hydroxysuccinimide ester biotin spacer arm was used for biotinylation of csso4-purified ev 11. biotinylation did not change the infectivity of the virus (attachment to and replication in susceptible cells). with the exception of ev ... | 1991 | 1667786 |
| in vivo significance of t cells in the development of coxsackievirus b3 myocarditis in mice. immature but antigen-specific t cells aggravate cardiac injury. | acute myocardial damage similar to that seen in human myocarditis occurs in balb/c mice after infection with coxsackievirus b3 (cb3) or encephalomyocarditis virus (emc). to investigate the role of antigen-specific t cells in the pathogenesis of this disorder, we compared cb3 disease expression in t cell-deficient, athymic nude (nu/nu) mice, in heterozygote (nu/+) mice with normal t cell function, and in nu/nu mice reconstituted with spleen cells from cb3- or emc-infected nu/+ mice. acute myocard ... | 1990 | 1697791 |
| reactivity of enterovirus-specific igm with infective and defective coxsackie b virions in patients with monotypic and multitypic igm responses. | the antigenic specificity of enterovirus (ev)-specific igm (ev-igm) responses in patients with acute enterovirus infection was studied using an igm-capture elisa (ev-igm elisa) employing coxsackie b virus types 1-5 (cbv 1-5) antigens. using antigens fractionated in caesium chloride (cscl) gradients, and monoclonal antibodies (mabs) and polyclonal antisera of defined specificity as detector antibody to detect igm-bound antigen, igm of single serotype specificity was shown to react consistently wi ... | 1990 | 1702793 |
| [virus demonstration and pathologic changes in different phases of coxsackievirus b myocarditis in mice]. | a/j mice between 15 days and 10 weeks of age were infected intraperitoneally with coxsackievirus b3 (cvb3). to search for virus in the myocardium various methods were applied: virus isolation from the myocardium, rna extraction for dot blot hybridization and in situ hybridization. two different rna probes, one specific for cvb3 the other cross-reacting with other enteroviruses, were radioactively labeled with 35s or 32p by in vitro transcription. in paraffin sections histological alterations wer ... | 1990 | 1708625 |
| identification of a putative shared epitope between coxsackie virus b4 and alpha cardiac myosin heavy chain. | molecular mimicry is an important postulated mechanism for autoimmunity in viral myocarditis. the 356-1 monoclonal antibody neutralizes coxsackie virus b4 by binding to the vp1 protein and cross-reacts with mouse alpha cardiac myosin heavy chain. we used this monoclonal antibody to screen a lambda gt11 expression library made from cd-1 mouse hearts. of the 48 positive plaques/10(6) recombinant phages examined, 14 of the strongest-reacting clones were purified for additional studies. the inserts ... | 1991 | 1717188 |
| anti-coxsackievirus b3 neutralizing antibodies with pathological potential. | adolescent cd-1 mice inoculated with coxsackievirus b3 (cvb3m) will develop acute myocarditis with focal lesions by 7 days post-inoculation (p.i.). administration of murine sera containing anti-cvb3m-neutralizing antibodies into cvb3m-inoculated mice at 3 days p.i. will exacerbate myocarditis, suggesting the presence of pathological antibodies. to study potential pro-inflammatory properties of virus-induced antibodies, a panel of anti-cvb3m-neutralizing monoclonal antibodies (mabs) was generated ... | 1991 | 1717270 |
| role of interferon in lethality and lymphoid atrophy induced by coxsackievirus b3 infection in mice. | to assess the importance of interferon (ifn) in the pathology of coxsackievirus b3 (cvb-3) infection, we evaluated both mortality rate and lymphoid involution in young adult balb/c mice infected with lethal doses of the virus and treated either with anti-ifn antibody or with murine ifn-alpha/beta. administration of antibody to ifn caused a profound worsening of the pathology and an increase in the mortality rate in infected animals. treatment with murine ifn exerted a significant ameliorative ef ... | 1991 | 1722097 |
| monoclonal antibody specific for the cellular receptor of echoviruses. | cell lines of primate origin carry membrane receptors which are specific for echoviruses (ev). the present report describes isolation and characterization of a monoclonal antibody (mab 143) reacting with the membrane of kb cells. the mab was selected for its protection of different cell lines from primate origin against the cpe of ev-11. this protection was found to extend to most ev serotypes and to coxsackievirus a9, while the replication of several other picornaviruses was not affected. the f ... | 1992 | 1740381 |
| autoanti-idiotypes exhibit mimicry of myocyte antigens in virus-induced myocarditis. | mice infected with coxsackievirus b develop immunologically mediated inflammatory myocarditis in heart tissue that results in the development of autoantibodies with multiple idiotypes. the specificity and temporal development of autoantibodies produced during coxsackievirus b3 infection were assessed. antiviral idiotypes and anti-idiotypic antibodies against coxsackievirus b3 idiotypes were detected and quantitated over 21- and 42-day periods, respectively. both polyclonal and monoclonal anti-id ... | 1991 | 1845881 |
| detection of coxsackievirus b3 rna in myocardial tissues by the polymerase chain reaction. | coxsackievirus b3 is a possible etiologic agent in some forms of myocarditis and idiopathic dilated cardiomyopathy. a method for the detection of coxsackievirus b3 rna was developed using the polymerase chain reaction based on the amplification of a cdna copy of the positive-strand viral rna. the fidelity of the method was established in two murine models for coxsackie b3 myocarditis. all cardiac specimens with adequate rna for study from coxsackie b3-infected mice contained detectable viral rna ... | 1991 | 1847008 |
| antibody to coxsackie b virus in diagnosing postviral fatigue syndrome. | to study the association between coxsackie b virus infection and the postviral fatigue syndrome and to assess the immunological abnormalities associated with the syndrome. | 1991 | 1847316 |
| selection of an attenuated coxsackievirus b3 variant, using a monoclonal antibody reactive to myocyte antigen. | previously, we described a heart-reactive monoclonal antibody (mab), 10a1, derived from a coxsackievirus b3 (cvb3)-infected mouse. this mab selectively inhibits infection of hela cells and myocytes with the myocarditic virus variant (cvb3w). a plaque-purified variant (h3) of cvb3w was isolated from the heart of an infected animal, and a second virus (h3-10a1) was obtained by growing h3 in hela cells in the presence of mab 10a1. as with the parental cvb3w virus, h3 infection of hela cells can be ... | 1991 | 1847455 |
| neutralization activity of some coxsackie b3 antibodies is compromised by protein factor(s) secreted by vero cells. | when various anti-coxsackie b3 virus antibodies were examined for the neutralizing activity in cultures under liquid medium, some antibodies including monoclonal antibodies gave abnormally low titers in the neutralization test in vero cells, in comparison with the other cells such as hela, fl, hep-2, or primary monkey kidney cells. the neutralization titer of these antibodies was, however, similar in all these cells by plaque reduction assays under agar overlay, i.e., the above phenomenon was re ... | 1991 | 1848064 |
| coxsackie b virus and postviral fatigue syndrome. | | 1991 | 1849432 |
| enteroviruses and idiopathic dilated cardiomyopathy. | previous studies have suggested that some cases of idiopathic dilated cardiomyopathy (idc) are due to persistent viral infection following an episode of viral myocarditis. viral rna sequences have recently been detected in material from patients with idc using molecular biological techniques. we tested 40 samples from recipients' hearts explanted at cardiac transplantation for the presence of enteroviral rna sequences, using a northern blotting technique. material from 19 cases of idc and 21 cas ... | 1991 | 1849988 |
| [epidemic of echovirus type 7 in kitakyushu city in 1986--a survey of isolation of viruses from the sewage and seroepidemiology of children]. | in 1986, under the infectious disease surveillance system in fukuoka prefecture, no isolation of echovirus type 7 from aseptic meningitis patients were reported. it was unclear that epidemics of this virus occurred in fukuoka prefecture, including kitakyushu city. we tried to isolate enteric viruses from the sewage (activated sludges) in kitakyushu city in 1986. outbreaks of isolations of echovirus type 7 started in september and continued until in december. this result suggested that echovirus ... | 1991 | 1919103 |
| [experimental data on relations of viral myocarditis and dilated cardiomyopathy]. | the aim of the given study is to simulate viral myocarditis (vmc) experimentally and to reveal morphologic evidences of its transformations into the dilatation cardiomyopathy (dcmp). syrian golden hamsters were infected with coxsackie b2 virus. optical and electron microscopic morphological changes of myocardium showed the possible transformation of vmc into dcmp. | 1990 | 1963335 |
| [coxsackie b virus infection]. | a case of a 27-year patient with a clinical syndrome of coxsackie b viral infection is presented. the symptoms included pleurodynia, myocarditis and the acute gastritis. the diagnosis was confirmed with serological tests which showed high titre of antibodies neutralizing coxsackie b3. the symptoms of myocardial infarction dominated in both clinical course and autopsy. the authors suggest that serological tests specific for coxsackie b infection should be performed in the young patients with the ... | 1990 | 1964729 |
| coxsackie b4 virus-induced changes in mouse pancreatic beta-cell mrnas. | | 1990 | 1965173 |
| arrhythmias in coxsackie b3 virus myocarditis. continuous electrocardiography in conscious mice and histopathology of the heart with special reference to the conduction system. | in patients with acute myocarditis, arrhythmias constitute one of the crucial risk factors for morbidity and mortality. to clarify the incidences and the chronological features of arrhythmias and their correlation with the histopathology of the heart, we conducted continuous electrocardiography in an animal model of viral myocarditis, and light microscopy of the heart with special reference to the conduction system. forty weanling c3h/he mice were divided into an inoculated group (ig) and a cont ... | 1990 | 1965542 |
| [isolation of viruses from autopsy material during the coxsackie virus epidemic in sarajevo in 1985]. | in this paper were presented results of isolation of vira from some organs of the dead newborn infants during the epidemy coxsackie b virosis in sarajevo in 1985. 12 newborn died. obduction was done in seven newborn. from the seven obducated, in six were isolated coxsackie b-3 vira from heart, lungs, brain, liquor, blood, heart blood but the attempts of isolation of the vira from intestine and from pericardial liquor did not succeed. | 1990 | 1965546 |
| [influence of dexamethasone on electrical activities in cultured rat beating myocardial cells infected with coxsackie b-2 virus]. | the effects of dexamethasone (dex) on electrical activities in cultured rat beating myocardial cells infected with 100 tcid-50 coxsackie b-2 virus (cb2v) was evaluated by conventional intracellular microelectrode technique. the frequency began to increase, the beating % decreased, and multiform arrhythmias were shown in the infected group 24 h post-challenge. meanwhile, the cytopathic effect (cpe) appeared rapidly from 1+ to 3+. in the infected and dex-treated group, the beating % was higher and ... | 1990 | 1966573 |
| enterovirus isolation from foetal and placental tissues. | four spontaneous abortions and two stillbirth occurred during a prospective survey following the teratogenicity of echoviruses in 80 pregnant women selected at random from the antenatal care service. echovirus types 19, 27, and 33. coxsackie b2 and b6 were isolated from placental and foetal tissues (brain, liver, kidney, heart, and spleen). the mothers also excreted the virus by faeces at least twenty days before abortion and responded serologically, indicating active virus infection. almost all ... | 1990 | 1975725 |
| [the miller-fisher syndrome during echo-7 neuro-infection. a case history]. | the authors describe a case of a 36-year old male patient with the miller fisher syndrome of ophthalmoplegia, ataxia and areflexia. an apparent etiopathogenetic factor in this case appears to be echo 7 virus. the pathological process was localized in the brainstem. | 1990 | 2131433 |
| cardiovascular lipid accumulation with coxsackie b virus infection in mice. | the authors used a myocarditic coxsackievirus b3 infection in balb/c mice to investigate cardiovascular lipid accumulation and whether a cholesterol-enriched diet influences the development of the myocardial inflammatory reaction. it was found that, seven days after cb3 infection, the accumulation of 14c-cholesterol increased by 75% (p less than 0.001) in the heart and by 92% (p less than 0.001) in the aorta. this infection also caused extensive inflammatory lesions (4.5% of tissue section area) ... | 1990 | 2153346 |
| specific detection of enteroviruses in clinical samples by molecular hybridization using poliovirus subgenomic riboprobes. | enteroviruses were specifically detected in crude clinical specimens or in cell cultures in which the viruses were amplified by dot hybridization by using poliovirus type 1-derived, subgenomic radiolabeled crna probes (riboprobes). the sensitivity of this test varied from 2.5 to 33%, when clinical specimens without cell culture were examined, and was about 85% in cell culture lysates. the specificity of the test was 90 to 100%. the riboprobe corresponding to the 5'-noncoding sequence specificall ... | 1990 | 2155917 |
| coxsackievirus-b3-induced myocarditis: virus receptor antibodies modulate myocarditis. | | 1990 | 2155966 |
| secondary enterovirus infection in the murine model of myocarditis. pathologic and immunologic aspects. | enteroviruses are implicated as etiologic agents in the inflammatory diseases myocarditis and polymyositis. in this report, we show that a previous enterovirus exposure in mice can influence development of myocardial inflammation with a second enteroviral exposure. inoculation of 25-day-old male c3h/hej mice with 10(3) or 10(5) plaque-forming units (pfu) of infectious or ultra violet (uv)-inactivated coxsackievirus b2 (cvb2), followed by inoculation 28 days later with 10(5) pfu of a myocarditic ... | 1990 | 2156432 |
| progressive interstitial collagen deposition in coxsackievirus b3-induced murine myocarditis. | myocardial fibrosis can be produced in certain inbred strains of mice after coxsackievirus b subtype 3 (cvb3) infection. the mechanism responsible for this interstitial matrix alteration is unknown. the presumption is that fibrosis occurs in areas of myocyte damage and inflammation associated with viral infection, analogous to scar formation after cell injury in other organ systems. to test this hypothesis, we examined the hearts of a/j strain mice infected with three cvb3 variants (crowell [cvb ... | 1990 | 2156433 |
| effect of enteroviruses on adherence to and invasion of hep-2 cells by campylobacter isolates. | coinfection of hep-2 epithelial cells with coxsackievirus b3, echovirus 7, poliovirus (lsc type 1), porcine enterovirus, and campylobacter isolates was performed to determine if a synergistic effect could be obtained. the invasiveness of campylobacter jejuni atcc 33560 was significantly increased for hep-2 cells preinfected with echovirus 7, coxsackievirus b3, and uv-inactivated (noninfectious) coxsackievirus b3 particles. additionally, the invasiveness of c. jejuni m96, a clinical isolate, was ... | 1990 | 2156779 |
| complete nucleotide sequence of infectious coxsackievirus b3 cdna: two initial 5' uridine residues are regained during plus-strand rna synthesis. | a full-length reverse-transcribed, infectious cdna copy of coxsackievirus b3 (cvb3) was used to determine the nucleotide sequence of this cardiotropic enterovirus. comparison of the nucleotide sequence and the deduced amino acid sequence of the viral precursor polyprotein with the sequences of other group b coxsackieviruses (cvb1 and cvb4) demonstrates a high degree of genetic identity. they share about 80% homology at the nucleotide level and about 90% when the amino acid sequences of the polyp ... | 1990 | 2157045 |
| echovirus 22 is an atypical enterovirus. | although echovirus 22 (ev22) is classified as an enterovirus in the family picornaviridae, it is atypical of the enterovirus paradigm, typified by the polioviruses and the coxsackie b viruses. cdna reverse transcribed from coxsackievirus b3 (cvb3) rna does not hybridize to genomic rna of ev22, and conversely, cdna made to ev22 does not hybridize to cvb3 genomic rna or to molecular clones of cvb3 or poliovirus type 1. ev22 cdna does not hybridize to viral rna of encephalomyocarditis virus or to a ... | 1990 | 2159539 |
| characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein vpg. | viral rna synthesis was assayed in hela cells transfected with nonviable poliovirus rna mutated in the genome-linked protein vpg-coding region. the transfecting rna was transcribed in vitro from full-length poliovirus type 1 (mahoney) cdna containing a vpg mutagenesis cartridge. hybridization experiments using ribonucleotide probes specific for the 3' end of positive- and negative-sense poliovirus rna indicated that all mutant rnas encoding a linking tyrosine in position 3 or 4 of vpg were repli ... | 1990 | 2159557 |
| evidence suggesting that virulence maps to the p1 region of the coxsackievirus b4 genome. | a chimeric virus containing the p1 region of a virulent variant of coxsackievirus b4 and the p2 and p3 regions of a nonvirulent strain was constructed from cdna clones. the chimeric virus induced pancreatitis with concurrent hypoglycemia similar to that observed in mice infected with the virulent variant. | 1990 | 2159563 |
| [study on the biological properties of solubilized hela cell receptors for group b coxsackieviruses]. | the properties of solubilized receptors for coxsackieviruses b (cbv) on hela cells were investigated. it was found that the receptor activity and antigenicity were comparatively sensitive to ph change and were rather stable over a wide range of temperatures (4 degrees c-60 degrees c) but they were destroyed completely when the temperature rose to 100 degrees c. oxidation and reduction augmented the receptor antigenicity, while the receptor activity was lowered only by oxidation and not by reduct ... | 1990 | 2161293 |
| immunodeficiency as primary phenotype of diabetes mutation db. studies with coxsackievirus b4. | restriction of food intake (r) in the c57bl/ksj db/db diabetic mutant mouse prevents phenotypic expression of diabetes, whereas ad libitum feeding (al) results in spontaneous diabetes. previous results showed that coxsackievirus b4 (cb4)-infected genetically identical db/db mice with and without diabetes could be distinguished by the levels of cb4-neutralizing antibody and virus-specific antibodies as determined by enzyme-linked immunosorbent assay and the numbers of splenic antibody-forming cel ... | 1990 | 2161367 |
| treatment of experimental coxsackie b-3 viral myocarditis with astragalus membranaceus in mice. | a murine model system for observing the effect of astragalus membranaceus (am) on experimental myocarditis caused by coxsackie b-3 virus (cb3v) was developed in 4-week-old male balb/c mice. gross, histopathologic and ultrastructural examinations of the infected-am treated group showed that the severity and involved area of the myocardial lesions became milder and smaller than those in the infected-ns treated mice. the total lesion area, and the total lesion area/total myocardial area examined (% ... | 1990 | 2161724 |
| persistence of enteroviral rna in chronic fatigue syndrome is associated with the abnormal production of equal amounts of positive and negative strands of enteroviral rna. | a subgenomic restriction fragment from cdna prepared from coxsackie b2 virus (cvb2) rna was subcloned into a riboprobe vector allowing the production of enteroviral group-specific rna probes complementary to either the positive (genomic) or negative (template) strand of enteroviral rna. these riboprobes were used to follow productive infection of cultured cells by cvb2; as expected, positive strand rna was synthesized in approximately 100-fold excess over negative strand. rna was extracted from ... | 1990 | 2161907 |
| experiments on antiviral activity of hand disinfectants. some theoretical and practical considerations. | various members of the picornavirus family, adenovirus 2, papovavirus sv40, as well as rotaviruses were tested for inactivation of infectivity by hand disinfectants formulated on the basis of ethanol. the inactivating effect of povidine-iodine (betaisodona) on enteroviruses and rotaviruses was also investigated. the degree of inactivation by these disinfectants on the various non-enveloped viruses studied, however, was found unpredictable. in fact, striking differences with the very same disinfe ... | 1990 | 2163645 |
| [late prognosis of viral myocarditis]. | 85 cases of virologically proved viral myocarditis were followed up for 96.45 +/- 23.7 months for a better understanding of the late prognosis of this disease. among these cases, 63 (74.1%) were cured, 14 (16.5%) remained with sequelae, 8 (3.5%) became chronic and 5 (5.9%) of the patients died. most of the abnormal findings, i.e. physical signs, x-ray, ecg and ucg changes, originally existing on admission of the survived patients disappeared. the clinical features of the chronic cases resemble t ... | 1990 | 2163732 |
| effect of astragalus membranaceus on electric activities of cultured rat beating heart cells infected with coxsackie b-2 virus. | astragalus membranaceus (am) which has a protective effect on rat beating heart cells infected experimentally with coxsackie b-2 virus was evaluated on the basis of changes in morphologic and electric activity of the cells. rhythm, beating frequency, beating percentage, cardiac cellular damage and cytopathic effects (cpe) were monitored every 24 h after challenge; electric activities parameters were measured by conventional intracellular microelectrode technique. significant protective effects w ... | 1990 | 2164463 |
| expression of major histocompatibility complex class i antigen in murine ventricular myocytes infected with coxsackievirus b3. | evidence has accumulated that t cell-mediated autoimmunity plays an important role in the pathogenesis of viral myocarditis. t lymphocytes are known to recognize antigen-presenting cells, such as virus-infected cells, being restricted by syngeneic major histocompatibility complex (mhc) antigens. to clarify in more detail the immunological mechanisms involved, we induced acute viral myocarditis in c3h/he mouse ventricles with coxsackievirus b3 (cvb3) and examined, by immunofluorescence, the expre ... | 1990 | 2165443 |
| coxsackie b4 viruses with the potential to damage beta cells of the islets are present in clinical isolates. | infections with coxsackie viruses (especially coxsackie b4) are thought to be involved in the pathogenesis of diabetes. many interdependent variables determine the outcome of an infection with a coxsackie virus, one of them being the tropism of the virus for a specific tissue. the extent to which beta cell tropic variants of coxsackie b4 virus occur naturally was assessed. human isolates of this virus were tested in an in vitro system in which elevated insulin release from infected islets incuba ... | 1990 | 2165944 |
| coxsackie b1 virus infection enhances the bacterial invasiveness, the phagocytosis and the membrane permeability in hep-2 cells. | to analyze the effect of coxsackie b1 virus infection on bacterial invasiveness, phagocytosis and cytoplasma membrane permeability, we have studied invasiveness of shigella flexneri, unspecific phagocytosis of latex particles and release of the non-metabolizible amino acid, alpha-aminoisobutyric acid (aib). virus infection enhanced invasiveness of s. flexneri and phagocytosis of latex beads and increased plasma membrane permeability as measured by release of aib. the effect on all three function ... | 1990 | 2166540 |
| a qualitative and quantitative method for in situ characterization of the inflammatory response in experimental myocarditis. | coxsackie virus b3 causes myocarditis in balb/c inbred mice. in this model mortality is about 60% on day 7 and 95% on day 12 after inoculation. significant inflammatory infiltrates appear on day 7. recent research has focused on the immune response to explain the ensuing tissue damage. thus we were interested in mapping cellular interaction and time course to understand the development of a possible autoimmune attack. by a newly developed immunohistochemical staining technique single lymphocytes ... | 1990 | 2166542 |
| evaluation of virus neutralization antibody levels in diabetic mutant mice. | experiments were designed to determine whether genetic predisposition to diabetes or overt diabetes in the identical genotype had an effect on the level of neutralization antibodies to coxsackievirus b4 (cb4). quantitation of neutralization antibody (nt) levels against cb4 was performed using both the 50% endpoint procedure and the multivariate wilcoxon rank sum test. the results of these experiments demonstrate that the use of the multivariate procedure for the analysis of neutralization antibo ... | 1990 | 2166746 |
| functional diversity in vascular endothelial cells: role in coxsackievirus tropism. | six plaque-purified virus isolates were obtained from liver and heart tissues of a dba/2 mouse infected 7 days earlier with 10(4) pfu of coxsackievirus group b type 3. each virus isolate was assayed in vitro for infectivity to vascular endothelial cells (vec) of the liver, lungs, and heart. both the percentage of vec infected and the mean progeny pfu produced per infected vec were determined. virus isolates from the heart showed greater infectivity and replication in heart vec than in vec derive ... | 1990 | 2166828 |
| induction of heterotypic virus resistance in adult inbred mice immunized with a variant of coxsackievirus b3. | infection of adult male c3h/hej mice with a host range variant of coxsackievirus b3 (cb3w-rd) induced resistance in these mice to an otherwise lethal dose of coxsackievirus b1 (cb1). the protective effect induced by cb3w-rd was detectable as early as 1 day post-vaccination and was still present 10 weeks later. while untreated mice infected with cb1 died within 5 days because of massive hepatic necrosis, the liver was spared in mice immunized with cb3w-rd and then challenged with cb1. in general, ... | 1990 | 2166894 |
| ventricular tachycardia associated with coxsackie b4 virus infection. | we describe a case of ventricular tachycardia in association with acute coxsackie b4 virus infection occurring in an otherwise normal infant. the dysrhythmia responded to flecainide acetate. | 1990 | 2166993 |
| a molecular and serologic evaluation of enteroviral involvement in human myocarditis. | murine coxsackie b virus infection models of myocarditis and numerous human serologic studies associating elevated enterovirus-specific igm titers with the clinical diagnosis of myocarditis have been used to support an etiologic role for enteroviruses in human myocarditis. of the human enteroviruses, coxsackie b viruses (cvb) are the enterovirus group most commonly associated with the human disease. while hybridization probes exist to detect most, if not all, human enteroviruses, no probe capabl ... | 1990 | 2167382 |
| coxsackie b antigen in the central nervous system of a patient with fatal acute encephalitis: immunohistochemical studies of formalin-fixed paraffin-embedded tissue. | a case of fatal acute encephalitis due to coxsackie b1 virus is described. confirmation of coxsackie b virus as the etiological agent of encephalitis was based on identification of the virus antigen in formalin-fixed paraffin-embedded tissue sections. in the past, the diagnosis was obtained by serological studies of peripheral blood and viral isolation. this is the first report in which indirect immunofluorescent and immunoperoxidase methods using rabbit antiserum raised against coxsackie b type ... | 1990 | 2167606 |
| immunosuppression with high doses of cyclophosphamide reduces the severity of myocarditis but increases the mortality in murine coxsackievirus b3 myocarditis. | to test the therapeutic efficacy of immunosuppression with cyclophosphamide (cyp) on coxsackievirus b3 (cb3) myocarditis, 2-week-old dba/2 mice were inoculated with 3 x 10(2) plaque-forming units of cb3 virus. cyp (100 mg/kg/day s.c.) was administered daily on days 0-8 (experiment 1; group 2), days 8-21 (experiment 2; group 4), and days 21-34 (experiment 3; group 6). groups 1, 3, and 5 were infected control groups for each experiment. spleen, thymus, and body weights were measured. in experiment ... | 1990 | 2168299 |
| species-specific substrate interaction of picornavirus 3c proteinase suballelic exchange mutants. | the substrate recognition properties of the polio-virus type 1 and coxsackievirus b3 3c proteinases have been examined in vitro by allelic and suballelic exchange of 3c between the cloned virus genomes. the activity of the altered 3c proteinases was examined by translation of synthetic rna in a rabbit reticulocyte lysate/hela cell extract translation system. analysis of the subsequent processing of virus polyproteins by the altered 3c proteinases showed that all of the mutant proteinases maintai ... | 1990 | 2168426 |
| vaccination of balb/c mice against enteroviral mediated myocarditis. | a non-virulent strain of coxsackie b3 (cb3) virus was used to produce a subunit vaccine. it contains the capsid proteins vp1, 2, 3 and probably 4 and can be made rna-free. it is based on the iscom technology ensuring non-toxic properties and good adjuvant effect. vaccinated animals at doses above 16 ng were completely protected from mortality when challenged with a myocarditic strain of cb3. histologically no inflammatory lesions were found in the heart. this was corroborated using immune histol ... | 1990 | 2168608 |
| [effects of coxsackie b-2 virus infection on electrophysiologic properties of beating cultured rat heart cells]. | beating rat heart cell cultures were prepared in vitro and infected with coxsackie b-2 virus. the cells were evaluated in the post infected period for changes in beating frequency, percentage of active cells and cytopathic effect (cpe), and for alterations of the electrical activity by standard intracellular microelectrode techniques. the beating frequency and percentage of active cells began to decrease in infected group at 24 h, and the beating percentage was less than 30% at 96 h after virus ... | 1990 | 2168827 |
| enhancement of coxsackievirus b3 replication in vero cells by indomethacin. | | 1990 | 2169504 |
| epidemic of fever of unknown origin in rural thailand, caused by influenza a (h1n1) and dengue fever. | in the late summer (rainy season) of 1987, a sharp outbreak of fever of unknown origin (fuo) in rural southern thailand was investigated by a field epidemiology team. in a random survey of households, 40 percent of the children and 20 percent of adults were reported to have had febrile illnesses within the last month. there was at least one death, possibly from reye's syndrome. testing 34 pairs of acute and convalescent sera showed significant hi antibody titer rises to influenza a (taiwan/(h1n1 ... | 1990 | 2169651 |
| detection and differentiation of picornaviruses in clinical samples following genomic amplification. | a polymerase chain reaction (pcr) assay was used to detect and differentiate picornaviruses (pvs), using primers homologous to the 5' non-coding and vp2 regions of the pv genome. the pcr resulted in a 530 bp pcr product for human rhinoviruses (hrvs) and a 650 bp product for polioviruses, coxsackieviruses (cv) or echoviruses. the pcr assay could detect as little as 1 p.f.u. of virus in either cerebrospinal fluid (csf) or stool, using ethidium bromide-stained gels. standard strains of poliovirus, ... | 1990 | 2170576 |
| effects of b4 coxsackievirus infection on cell-mediated immunity, natural cytotoxicity and interleukin-1 production by spleen cells, in mice. | by using cba inbred mice as responders, the influence of in vivo b4 coxsackievirus infection (in two separate experimental procedures) on anti-virus cell-mediated immunity (cmi), natural killer (nk) cell activity and lipopolysaccharide (lps) induced interleukin-1 (il-1) production in spleen cells were studied. both experimental infection schedules were able to induce a "positive" cmi response. nk cell activity was also significantly stimulated, especially by the administration of high virus amou ... | 1990 | 2171627 |
| [diversity of etiological agent associated with aseptic meningitis--a survey on an epidemic in tajimi city, gifu prefecture in 1984]. | during the period from may to september in 1984, an epidemic of aseptic meningitis occurred in tajimi city, gifu prefecture. virological and serological investigations were performed. cases involved ranged from 0 to 12 years of age, and 83.1% of them were under 7 years of age. four types of enteroviruses were isolated from 38 to 59 cases (64.4%), and virus type isolated were coxsackie b1 (15 cases), b4 (2 cases), b5 (4 cases), echovirus 16 (14 cases), a mixture of coxsackie-virus b4 and echoviru ... | 1990 | 2172413 |
| [neutralizing antibodies to coxsackie virus group b 1986-1988]. | presence of neutralizing antibodies to coxsackie b viruses (cbv) 1-5 were examined in 2885 human sera by the neutralization test. the most frequently detected antibodies were to b4 viruses (in 1495 sera--51.82%) and to b3 (1371--47.52%). antibodies to other serotypes were found in lower percentage: to b1 in 387 samples (13-41%), to b2 in 404 sera (14%) and in b5 in 332 sera (11.5%). presence of neutralizing antibodies to human cbv in heart diseases were also analysed in 67 serum pairs. in this g ... | 1990 | 2173272 |
| removal of pathogenic human viruses by insoluble pyridinium-type resin. | cross-linked poly(n-benzyl-4-vinylpyridinium bromide) (bvp resin) was found to be very efficient in removing pathogenic human viruses from aqueous solution. in batch removal experiments using 50 g/l of bvp resin at 35 degrees c, the level of infectivity in suspensions of enterovirus, herpes simplex virus, poliovirus, and human immunodeficiency virus was reduced 1000-100,000 fold during a 2 h period. those of coxsackievirus and echovirus were reduced 60-600 fold during 1 h contact. the haemagglut ... | 1990 | 2174373 |
| [experimental glomerulonephritis induced by coxsackie b4 virus in mice--glomerular changes associated with intermittent viral inoculations]. | it has been considered that viruses may be implicated in the genesis and progression of glomerular diseases. several experimental and clinical studies have reported nephritogenecity of coxsackie (cox.) b4 virus infection. so in order to elucidate glomerulopathy of intermittent cox. b4 virus infection, one-month-old swiss albino mice were inoculated intravenously with purified cox. b4 virus, 3 x 10(7) tcd50, once a month, and then were sacrificed from 4 to 9 mo. of age (from 3 to 8 times inoculat ... | 1990 | 2175817 |
| detection of immunoglobulin g, m, and a antibodies to enterovirus structural proteins by immunoblot technique in echovirus type 4-infected patients. | paired serum specimens from 24 patients with echovirus (ev) type 4 infection by virus isolation were tested by the immunoblot technique for the presence of igg, igm, and iga antibodies to ev4 structural proteins. single sera from 20 patients without neutralizing enterovirus igm were used as controls. all the sera from ev4-infected patients had igg antibodies to vp1 of ev4 but also 13 out of the 20 controls. 23 out of 24 ev4-infected patients elicited igm and iga specific antibodies to vp1, a pat ... | 1990 | 2176661 |
| [influence of coxsackie b4 virus on uptake and transport of colloidal carbon by glomerular mesangium]. | to evaluate the influence of coxsackie b4 virus (cox. b4 virus) on the clearing function of the mesangium, colloidal carbon and cox. b4 virus were intravenously injected into five groups of female swiss albino mice. group i received carbon and no cox. b4 virus; group ii, carbon and live cox. b4 virus; and group iii, carbon and inactivated cox. b4 virus; group iv and v received live and inactivated cox. b4 virus, respectively, first, followed by carbon. semiquantitative analysis of the distributi ... | 1990 | 2177114 |
| antigenic competition between dengue and coxsackie viruses for presentation to b cells by macrophages. | macrophages (m phi) pulsed with dengue type 2 (dv) and coxsackie b4 (coxb) viruses present antigen to b lymphocytes leading to their clonal expansion as detected by counting antigen-specific igm antibody plaque-forming cells (pfc). the present study was undertaken to investigate the site for competition in m phi between the two heterologous antigens, dv and coxb, for their presentation to b cells. it was observed that dv-pulsed m phi presented antigen to b cells in mice depleted of t cells by tr ... | 1990 | 2177622 |
| the viral genome in experimental murine coxsackievirus b3 myocarditis: a northern blotting analysis. | the presence of viral genomes in the murine heart in experimental coxsackievirus b3 myocarditis was investigated by northern blotting analysis. four-week-old c3h/he mice were inoculated with coxsackievirus b3. after sacrifice, the hearts were divided into 3 parts. total rna was extracted from one part of the heart. the other two parts were used for investigation of histopathology and of virus titer by plaque assay. the 32p-labeled cdna probe was derived from the 5' end sequence of the coxsackiev ... | 1990 | 2177795 |
| impact on routine diagnosis of echovirus infections of intratypic differentiation and antigenic variation in echovirus type 25 studied by using monoclonal antibodies. | we studied the biological and antigenic properties of wild strains of echovirus type 25 isolated in france between 1982 and 1987 and compared them with the jv-4 prototype strains isolated in 1957. the wild strains differed from the prototype strain in their cellular tropism. the prototype strain grew readily in five cell lines (mrc5, ma 104, vero, bgm, and ht 29-18), while for wild strains mrc5 and ht 29-18 cells were the most sensitive and supported growth to high titres (between 4.5 and 7.4 50 ... | 1990 | 2229354 |