| the primary structure of bacillus subtilis and bacillus stearothermophilus 5 s ribonucleic acids. | the nucleotide of bacillus stearothermophilus 5 s rna is pc-c-u-a-g-u-g-a-c-a-a-u-a-g-c-g-(g-a-g-a-g-g-)-a-a-a-c-a-c-c-c-g-u-u-c-c-c-a-u-c-c-c-g-a-a-c-a-c-g-g-a-a-g-u-u-a-a-g-c-u-c-u-c-c-a-g-c-g-c-c-g-a-u-g-g-u-a-g-u-u-g-g-g-g-c-c-a-g-c-g-c-c-c-c-u-g-c-a-a-g-a-g-u-a-g-g-u-c-g-u-u-g-c-u-a-g-g-coh; the nucelotide sequence of bacillus subtilis 5 s rna is pu-u-u-g-g-u-g-g-c-g-a-u-a-g-c-g-a-a-g-a-g-g-u-c-a-c-a-c-c-c-g-u-u-c-c-c-a-u-a-c-c-g-a-a-c-a-c-g-g-a-a-g-u-u-a-a-g-c-u-c-u-u-c-a-g-c-g-c-c-g-a-u-g ... | 1976 | 818086 |
| [dynamics of growth of mesophilic and thermophilic microflora from hot springs]. | samples containing mesophilic and thermophilic bacteria from a natural thermal spring were inoculated into a medium made with the water of the same spring. the growth rate of the thermophilic microflora was higher but the mesophilic microflora produced more biomass than the thermophilic bacteria. | 1975 | 818483 |
| a comparison of the 16s ribosomal rnas from mesophilic and thermophilic bacilli: some modifications in the sanger method for rna sequencing. | two modifications in the sanger two dimensional electrophoretic procedure for rna analysis are reported. one increases resolution on the primary fingerprint to the point that digests of large rnas, of the size 1500-3000 nucleotides yield well resolved fingerprint patterns. the other is a novel endonucleolytic procedure that proves useful in determining sequences of the large oligonucleotides produced by t1 ribonuclease. these modifications have been used in determining the catalogs of oligomers ... | 1976 | 819656 |
| [the physiology of thermophilic and mesophilic bacilli during development at optimal and submaximal temperatures]. | thermophilic bacilli, contrary to mesophilic, grow equally well under aerobic and anaerobic conditions. this may be due to the presence of more active anaerobic dehydrogenases in thermophilic organisms. the activity of glucose-6-phosphate dehydrogenase and alcohol dehydrogenase, and the content of atp, increased in the cells of thermophilic bac. stearothermophilus 159 and mesophilic bac. megaterium m strains growing at temperatures close to maximal. the activity of glutamate dehydrogenase under ... | 1976 | 819761 |
| protein synthesis at 680 atm: is it related to environmental origin, physiological type, or taxonomic group? | the ability of bacteria to synthesize protein at 680-atm pressure is not related to their environmental origin, physiological type, or taxonomic group. | 1976 | 820255 |
| thermophilic glyceraldehyde-3-p dehydrogenase. | | 1976 | 820563 |
| role of calcium ion in the thermostability of alpha-amylase produced from bacillus stearothermophilus. | | 1976 | 820570 |
| [drug residues in poultry (author's transl)]. | the following microbiological tests: the bacillus subtilis bga (bundes-gesundheitsamt) test and sarcina lutea test as well as the test bacteria bacillus stearothermophilus var. calidolactis and e. coli were used to examine whether drug residues were detectable in broiler chickens during and after treatment with various drugs. with the exception of the occidiostat esb3, residues were not found to be present using the two above tests. on the other hand, residues of a number of drugs were detected ... | 1976 | 821173 |
| evaluation of a new design of bottle closure for non-injectable water. | | 1976 | 821906 |
| [regulation of citrate synthese in bacteria: comparison of the action of various effectors on the enzymes of rhodospirillum rurbum and bacillus stearothermophilus]. | a comparative study of the citrate synthases purified from the facultatively photosynthetic bacterium rhodospirillum rubrum (gram negative) and the thermophile bacillus stearothermophilus (gram positive) was made. the citrate synthase from r. rubrum was activated by kcl (6-fold at 0.1 m kcl) and, less effectively, by nacl and nh4cl. its molecular weight was about 300,000. the enzyme was strongly inhibited by nadh, and this inhibition was counteracted by amp. the citrate synthase from b. stearoth ... | 1976 | 826987 |
| the binding site of escherichia coli ribosomal protein s4 on 16-s ribosomal rna from different bacterial species. | ribosomal protein s4 of escherichia coli was bound to 16-s ribosomal rnas from several bacterial species and the complexes digested with pancreatic ribonuclease in an effort to isolate heterologous rna binding sites for protein s4. 16-s rnas from aeromonas punctata, pseudomonas fluorescens and vibrio cuneatus each gave rise to protected fragments whose electrophoretic mobility was 7s, i.e. similar to that of the fragment generated from e. coli 16-s rna using the same conditions. no comparable fr ... | 1976 | 827436 |
| thermophilic aminopeptidase i. | | 1976 | 827664 |
| purification and properties of homoprotocatechuate 2,3-dioxygenase from bacillus stearothermophilus. | the enzyme 3,4-dihydroxyphenylacetate:oxygen 2,3-oxidoreductase (decyclizing) (homoprotocatechuate 2,3-dioxygenase) was purified from the thermophilic organism bacillus stearothermophilus, grown with j-hydroxyphenylacetic acid as a source of carbon. the enzyme appeared to be homogeneous as judged by disc-gel electrophoresis and sedimentation equilibrium measurements. the average molecular weight determined by three independent procedures was 106,000; the protein was globular and was dissociated ... | 1977 | 838683 |
| the use of alpha-galactosidase and invertase in hollow fiber reactors. | invertase and alpha-galactosidase have been immobilized in hollow fiber cartridges with no detectable enzyme leakage and used for the hydrolysis of sucrose and raffinose, respectively. for both hollow fiber immobilized enzymes nearly complete substrate conversion is possible. enzyme stabilities in polysulfonate hollow fibers which have been preconditioned with bovine albumin approach the stabilities of the free enzymes. | 1977 | 843613 |
| dna polymerase from mesophilic and thermophilic bacteria. iii. lack of fidelity in the replication of synthetic polydeoxyribonucleotides by dna polymerase from bacillus licheniformis and bacillus stearothermophilus. | 1. dna polymerase from the mesophile bacillus licheniformis and the thermophile bacillus stearothermophilus has been used to study the replication of poly(da-dt)-poly(da-dt) and poly(dc)-poly(dg) templates at 37, 45, and 55 degrees c. 2. incorporation of non-complementary deoxyribonucleoside triphosphates (misincorporation) occurred with both enzymes and both templates. non-specific incorporation (de novo polynucleotide synthesis, random attachment to existing strands, and tritium exchange of nu ... | 1977 | 849445 |
| folding domains and intramolecular ionic interactions of lysine residues in glyceraldehyde 3-phosphate dehydrogenase. | 1. treatment with methyl acetimidate was used to probe the topography of several tetrameric glyceraldehyde 3-phosphate dehydrogenases, in particular the holoenzymes from rabbit muscle and bacillus stearothermophilus. during the course of the reaction with the rabbit muscle enzyme, the number of amino groups fell rapidly from the starting value of 27 per subunit to a value of approx. five per subunit. this number could be lowered further to values between one and two per subunit by a second treat ... | 1977 | 851424 |
| thermophilic aminopeptidase. iv. cooperative effects in ans binding by the thermophilic aminopeptidase i from b. stearothermophilus. | aminopeptidase i is a membrane-bound metalloenzyme isolated from b. stearothermophilus which is thermostable and requires co2+ for activity. the co, zn, and metal-free enzyme were titrated with ans, and cooperative binding was noted with the active co enzyme but not with the zn (5% active) or apo (inactive) enzymes. there are a number of sites for ans on each of the three enzyme forms and the agreement between the association constants of the zn enzyme (identical and independent sites) and the n ... | 1977 | 856749 |
| preliminary x-ray diffraction studies of orthorhombic crystals of superoxide dismutase from bacillus stearothermophilus. | | 1977 | 875027 |
| [a microbiological method for the rapid determination of antibiotic concentrations during therapy (author's transl)]. | the assay described is an agar diffusion test using bacillus stearothermophilus as test organism. incubation is at 62 degrees c; results can be obtained rapidly within 2 to 3 hours. the test was developed for the determination of gentamicin concentrations in serum and other body fluids. because of its high sensitivity the test strain is also suitable for the determination of other antibiotics. the test is designed primarily for the determination of concentrations in individual samples and thus f ... | 1977 | 881260 |
| a pulse-radiolysis study of the manganese-containing superoxide dismutase from bacillus stearothermophilus. a kinetic model for the enzyme action. | the enzymic reaction mechanism of a manganese-containing superoxide dismutase from bacillus stearothermophilus was studied by using pulse radiolysis. during catalysis (ph 8.9; 25 degrees c), changes occurring in the kinetics of substrate disappearance and in the visible absorption of the enzyme at 480 nm established that the simple two-step mechanism found for copper- and iron-containing superoxide dismutases is not involved. at a low ratio (less than 15) of substrate concentration to enzyme con ... | 1977 | 889577 |
| the binding site of ribosomal protein s8 on the 16-s ribosomal rna from bacillus stearothermophilus. | | 1977 | 891539 |
| temperature-induced metabolic alterations in a thermophilic bacillus. | | 1977 | 891545 |
| the amino acid sequence of tryptophanyl trna synthetase from bacillus stearothermophilus. | | 1977 | 891985 |
| detection of residual penicillins in milk by using a bacillus stearothermophilus disk assay. | a paper disk method based on a procedure described by the international dairy federation is presented for the detection of 4 beta-lactam antibiotics (penicillin g, ampicillin, cephapirin, and cloxacillin) in whole milk. bacillus stearothermophilus var. calidolactis, prepared as a stable spore suspension, was used as the test organism. levels of 0.005 unit penicillin g/ml, 0.005 microgram ampicillin or cephapirin/ml, and 0.05 microgram cloxacillin/ml were readily detected in whole milk. results w ... | 1977 | 893331 |
| superoxide dismutase from bacillus stearothermophilus: reversible removal of manganese and its replacement by other metals [proceedings]. | | 1977 | 923975 |
| new rapid gel sequencing method for rna. | | 1977 | 927512 |
| aminopeptidase ii from bacillus stearothermophilus. | 1. the low molecular weight aminopeptidase (aminopeptidase ii) from bacillus stearothermophilus cells grown at 50 degrees c was purified to a homogeneous state. 2. molecular weight determination by sodium dodecyl sulfate gel electrophoresis resulted in a value of 46 000 for the subunits. a molecular weight of 80 000-100 000 has been reported for the native enzyme. we therefore conclude that aminopeptidase ii is a dimeric enzyme. 3. the amino-terminal sequence, the amino acid analysis and the sub ... | 1976 | 938681 |
| purification and catalytic properties of "thermostable" fumarase from bacillus stearothermophilus nu-10 and thermus x-1. | fumarase (l-malate hydro-lysase e.c.4.2.1.2) was purified from the thermophilic bacteria bacillus stearothermophilus nu-10 (optimum growth temperature 62-63 degrees c) and thermus x-1 (optimum growth temperature 70 degrees c). the furmarase from thermus x-1 is slightly more thermostable and has an "optimum" catalytic reaction temperature of 83 degrees c as compared to 81 degrees c for the b. stearothermophilus enzyme. increased thermostability of these fumarases permitted an examination of the p ... | 1976 | 939272 |
| anaplerotic enzymes of acetate and pyruvate metabolism: distinctive characteristics in bacillus stearothermophilus. | | 1976 | 939275 |
| ferredoxin from bacillus stearothermophilus. | | 1976 | 939276 |
| purification, characterization and mechanism of action of several aminoacyl-trna synthetases from bacillus stearothermophilus. | | 1976 | 939278 |
| thermophilic and mesophilic enzymes from b. caldotenax and b. stearothermophilus: properties, relationships and formation. | 1) the adaptive system of thermophilic bacteria, as demonstrated with b. caldotenax, seems to be suitable to produce thermophilic and mesophilic enzymes for comparative studies. 2) if it may be assumed that the extensive homologies in the n-terminal sequences of the ldhs also extend over the entire polypeptide chain, comparison of these sequences together with investigation on the 3-dimensional structure offer the possibility of elucidating those structural details which may be responsible for t ... | 1976 | 939279 |
| metal-replacement studies in bacillus stearothermophilus aldolase and a comparison of the mechanisms of class i and class ii aldolases. | a comparison of the product-inhibition patterns during cleavage of d-fructose 1,6-diphosphate by aldolases from yeast, rabbit muscle and bacillus stearothermophilus shows an ordered reaction sequence for all three enzymes, with dihydroxyacetone phosphate the last-leaving product. addition of zn2+, co2+, fe2+, mn2+ or cd2+ ions to the inactive apo-(bacillus stearothermophilus aldolase) restores activity to different extents, whereas ni2+, mg2+ or cu2+ ions have no effect. the cleavage activity of ... | 1976 | 942370 |
| superoxide dismutase from bacillus stearothermophilus: crystallization and preliminary x-ray diffraction studies. | | 1976 | 966282 |
| the production of spores of bacillus stearothermophilus with constant resistance to heat and their use as biological indicators during the development of aqueous solutions for injection. | | 1976 | 967909 |
| effect of storage time and temperature and the variation among replicate tests (on different days) on the performance of spore disks and strips. | laboratory-prepared spore disks were stored for 96 weeks at 22 degrees c with 50% relative humidity (rh) and at 4 degrees c with less than 1% rh. at the same time commercial spore strips were stored for 64 weeks at 22 degrees c with 50% rh. the spore count per unit and the heat resistance were measured at the beginning of the experiment and after 16, 32, 48, 64, 80, and 96 weeks of storage. the laboratory-prepared spore disks stored at 4 degrees c with less than 1% rh showed less change in numbe ... | 1976 | 970944 |
| the crystal structure of tyrosyl-transfer rna synthetase at 2-7 a resolution. | | 1976 | 972395 |
| a rapid microbiological procedure using bacillus stearothermophilus for the assay of antibacterial drugs. | | 1976 | 977788 |
| [biological and chemical indicators of thermal sterilization and their possible effect on its general concept]. | regarding to ignorance of germ thermoresistance occurring in hospital environment, the effect of sterilization in medicine is determined occasionally on samples of medium with high number of germs or tests are performed to determine the sterilization success (in pharmacy) by examining samples of sterile badges, although their degrees of contamination (mostly low) before sterilization are not known. instead of fixed sterilization parameters, the new second pharmacopoeia of the german democratic r ... | 1976 | 987099 |
| the detection of penicillin in buttermilk. | when 59 buttermilk samples were tested for the presence of antibiotics or chemostherapeutic substances by a method using the spores of bacillus stearothermophilus var. chalidolactis all samples gave a positive reaction corresponding to about 0.01 iu penicillin per ml. when the buttermilk samples were diluted 1:1 (v/v) by a phosphate buffer, ph 6.8, only 2 were positive which were confirmed to contain penicillin. the mean ph of the unbuffered buttermilks was found to be 4.53 +/- 0.11 and when buf ... | 1976 | 989169 |
| [inhibitory action of the residues of some new disinfecting agents on the microflora of bulgarian milk]. | studied were the amounts of residues of disinfection solutions such as antigerm 0.3 per cent, bradophen 0.2 per cent, abosanit 0.35 per cent, trosilin 0.5 per cent, and oxania 2 per cent which could produce inhibitory effects of the microflora of the bulgarian sour milk. it was established that the presence of more than 25 ml/1 of a given disinfection solution in the milk of the above-mentioned ones at the concentrations cited (with the exception of trosilin) exert a strong inhibitory action on ... | 1976 | 989201 |
| the citrate synthase from bacillus stearothermophilus. | citrate synthase has been purified to apparent homogeneity from bacillus stearothermophilus. its kinetic and regulatory properties, and molecular weight, are similar to those of the enzymes from suitable mesophilic counterparts, but its thermal stability is considerably greater. | 1976 | 991973 |
| amino acid sequence of a four-iron-four-sulphur ferredoxin isolated from bacillus stearothermophilus. | 1. the primary structure of a 4fe-4s ferredoxin from bacillus stearothermophilus was determined and shown to consist of a single polypeptide chain of 81 amino acid residues. the molecular weight of the holoprotein is about 9120. 2. there are only four cysteine residues in the molecule; three of these are located near the n-terminus as a cys-x-x-cys-x-x-cys segment, and the fourth cysteine residue is followed by a proline and located in the c-terminal half. 3. the fe-s chromophore in b. stearothe ... | 1976 | 999643 |
| formation of 5,5-dimethyl-delta2-thiazoline-4-carboxylic acid during cleavage of penicillin g by d-alanine carboxypeptidase from bacillus stearothermophilus. | | 1976 | 1002716 |
| superoxide dismutase from bacillus stearothermophilus. preparation of stable apoprotein and reconstitution of fully active mn enzyme. | | 1976 | 1003466 |
| degradation of penicillin g to phenylacetylglycine by d-alanine carboxypeptidase from bacillus stearothermophilus. | d-alanine carboxypeptidase from bacillus stearothermophilus is a membrane-bound enzyme which is inhibited by covalent interaction with penicillin g. the penicilloyl enzyme spontaneously reactivates and simultaneously releases a penicillin g degradation product; 0.2 mumol of the latter was isolated after incubation of 4.2 mumol of [8-14c]penicillin g with 10 g of membrane protein. it was identified as phenylacetylglycine by chromatographic techniques, infrared spectroscopy, and mass spectrometry. ... | 1975 | 1059132 |
| lack of ribosomal protein s1 in bacillus stearothermophilus. | the 30s ribosomal subunit of bacillus stearothermophilus migrated as a single band when electrophoresed on agarose-acrylamide composite gels. the addition of the ribosomal protein s1 purified from escherichia coli resulted in the appearance of an additional band migrating more slowly; 14c-labeled s1 of e. coli was shown to be associated only with this form. antibody against e. coli protein s1 did not crossreact with either the total 30s ribosomal proteins or the postribosomal supernatant from b. ... | 1976 | 1062787 |
| hybridization of glyceraldehyde-3-phosphate dehydrogenase. | 1. glyceraldehyde-3-phosphate dehydrogenases [ec 1.2.1.12] from rabbit, pig, lobster, yeast, e. coli, and b. stearothermophilus have been subjected to hybridization in 3m nacl. 2. suitable mixtures of electrophoretically distinct glyceraldehyde-3-phosphate dehydrogenases were found to give five-membered by electrophoresis on cellulose acetate. 3. the thermophile enzyme did not form hybrids with any of its mesophile counterparts, presumably because it does not dissociate under the conditions used ... | 1975 | 1097430 |
| role of ribosomal protein s1 in portein synthesis: effects of its addition to bacillus stearothermophilus cell-free system. | products of the f2 phage rna-directed protein synthesizing systems in vitro, derived from escherichia coli and bacillus stearothermophilus, were analyzed. in contrast to other reports, it was found that b. stearothermophilus ribosomes synthesized phage coat protein when the high-speed (100 000 x g) supernatant (s100) and ribosomal wash (crude initiation factors) of homologous origin were used. furthermore, a marked stimulation of coat protein synthesis was observed with b. stearothermophilus rib ... | 1975 | 1100388 |
| carboxyl-terminal sequences of procaryotic ribosomal proteins from escherichia coli, bacillus stearothermophilus, and halobacterium cutirubrum. | the carboxyl-terminal amino acid sequences of two ribosomal proteins, escherichia coli l12 and e. coli s4, and the proteins believed to be their equivalents from bacillus stearothermophilus and halobacterium cutirubrum, were determined. these proteins are known to be required for peptide chain termination (l12) and in ribosome assembly (s4). the carboxyl-terminal sequences obtained suggest that the e. coli and b. stearothermophilus proteins have retained structural homology in this region, where ... | 1975 | 1102067 |
| denaturation of thermophilic and mesophilic 6-phosphogluconate dehydrogenase by 8m urea. | the denaturation reaction of 6-phosphogluconate dehydrogenase (6-phospho-d-gluconate: nadp oxidoreductase (decarboxylating ec 1.1.1.44) from b. stearothermophilus and e. coli in 8 m urea has been compared. the rates of denaturation were evaluated from the fluorescence quenching that occurs at 334 nm. the thermophilic enzyme has been found about ten times more resistant to the action of the denaturating agent, and showed a biphasic denaturation process, whereas the e. coli enzyme followed a singl ... | 1975 | 1102472 |
| temperature related alterations in the acidic alanine-rich "a" protein from the 50s ribosomal particle of the extreme halophile, halobacterium cutirubrum. | 50-s ribosomal subunits from the extreme halophilic bacterium, halobacterium cutirubrum, contain an alanine-rich acidic "a" protein which resembles the l7--l12 multimer (kaltschmidt and wittmann, 1970) found in the 50-s ribosomal subunit of escherichia coli cells. the protein contains 24 mole % alanine and is devoid of histidine, tryptophan and cysteine. unlike e. coli which has two forms of the "a" protein distinguished solely by the acetylation state of the serine amino terminus. h. cutirubrum ... | 1975 | 1102949 |
| comparative thermostability of thermophilic and mesophilic 6-phosphogluconate dehydrogenase. | | 1975 | 1104459 |
| observations on environmental contamination in a microbiological laboratory. | contamination of a laboratory environment with pathogenic or non-pathogenic micro-organisms may be relevant to safety of technicians and quality of technical performance. two widely separated incidents in 1968 and 1974 initiated a study of aspects of the laboratory environment. water-baths, water of syneresis and portions of salmonella cultures spurting out of the sterilizing flame were examined. the water of water-baths was shown to be contaminated from the fluid cultures incubated in them. thi ... | 1976 | 1107415 |
| biological indicators for steam sterilization process monitoring. | | 1975 | 1131456 |
| effect of holding time in parenteral solutions on the outgrowth of bacillus stearothermophilus spores. | | 1975 | 1131458 |
| mutants of bacillus stearothermophilus de-repressed for isocitrate lyase and malate synthase. | | 1975 | 1132564 |
| comparison by photoaffinity labeling of the proteins involved in gtp hydrolysis from 70s ribosomes and a 5s rna-protein complex from bacillus stearothermophilus. | | 1975 | 1137594 |
| aminoacyl-trna synthetases from bacillus stearothermophilus. asymmetry of substrate binding to tyrosyl-trna synthetase. | the interaction of l-tyrosine, l-tyrosyladenylate and trna-tyr with tyrosyl-trna synthetase from bacillus stearothermophilus was studied by equilibrium dialysis, gel filtration and fluorescence spectroscopy. the enzyme, which consists of two identical subunits (mol. wt 2 x 44000), binds only a single molecule of l-tyrosine per dimer with a k-d of 2 x 10-5 m at ph 7.8 and 23 degrees c. the tyrosyl-trna synthetase--tyrosyladenylate complex which was isolated by gel filtration also has one adenylat ... | 1975 | 1140198 |
| behaviour of bacillus stearothermophilus grown in different media. | | 1975 | 1141075 |
| utilization of phenol and cresols by bacillus stearothermophilus, strain ph24. | | 1975 | 1141860 |
| types and distribution of obligate thermophilic bacteria in man-made and natural thermal gradients. | the types and distribution of obligate thermophilic bacteria were found to be similar in a thermal gradient resulting from man-made thermal pollution and the thermal gradients of two natural hot springs located in colorado. | 1975 | 1147612 |
| association of ribosomal subunits. iv. polyamines as active components of the association factor from bacillus stearothermophilus. | the association of ribosomal subparticles induced by several associating agents has been studied under different conditions. the following observations were made: 1. spermidine was able to produce the association of subunits, and the concentration and temperature curves of this reaction were similar to those obtained with association factor. the product formed in the latter case was more stable. 2. the association at low mg2+ concentration was higher with association factor than with polyamines. ... | 1975 | 1148242 |
| [oxidation of phenol by bacillus stearothermophilus strains]. | the assimilation of phenol as a sole source of carbon and energy was studied with the thermophilic bacillus isolated from the geothermal zones in the south ural. this ability was displayed by 11 strains of bacillus stearothermophilus among 26 studied strains. the most active strains oxidized all phenol, when its content in the medium was 0.1--0.2%, during two days, at 56--58 degrees c, with aeration; a considerable amount of biomass was accumulated and the medium was acidified. the maximum conce ... | 1975 | 1160654 |
| [thermostability of d-glyceraldehyde 3-phosphate dehydrogenase and phosphoglycerate kinase of b. stearothermophilus (author's transl)]. | | 1975 | 1170597 |
| [thermostability of alpha-amylase produced from thermophilic bacterium (author's transl)]. | | 1975 | 1170598 |
| binding behaviour of pyruvate carboxylase from bacillus stearothermophilus on sepharose--avidin. | | 1975 | 1175777 |
| development of defined and minimal media for the growth of bacillus stearothermophilus. | defined media, both solid and liquid, that support good growth of bacillus stearothermophilus 1503 have been developed. data are presented which indicate that manganese is required at relatively high concentrations for growth in a defined liquid medium. phosphate concentrations higher than 5 times 10(-3) m have been shown to inhibit colony formation on solid media. maximum viable counts of approximately 10(9) colony-forming units per ml were obtained in both the defined and minimal liquid media. ... | 1975 | 1176432 |
| production of alcohol by bacillus stearothermophilus. | | 1975 | 1182284 |
| metabolism of phenol and cresols by bacillus stearothermophilus. | an obligate thermophilic strain of bacillus stearothermophilus, strain ph24, isolated from industrial sediment by elective culture, grew readily at 55 c on phenol or on one of the isomers of cresol as the major carbon source. intact cells grown in the presence of phenol, o-cresol, m-cresol, or p-cresol were induced to oxidize, without lag, these substrates together with catechol, 3-methylcatechol, and 4-methylcatechol. cell extracts prepared from b. stearothermophilus ph24 after growth in the pr ... | 1975 | 1194230 |
| identification of a component of crystalline egg albumin bactericidal for thermophilic aerobic sporeformers. | during an investigation of the effect of basic and acidic proteins on the growth of thermophilic aerobic sporeformers, crystalline egg albumin was found to be strongly bactericidal. this finding was uncharacteristic of acidic proteins. the bactericidal fraction was heat sensitive and separated from the non-bactericidal albumin fraction during gel filtration on sephadex g-75. cells of micrococcus lysodeikticus and bacillus stearothermophilus were lysed rapidly by the bactericidal component, leadi ... | 1975 | 1200631 |
| stability of protein and ribonucleic acid in bacillus stearothermophilus. | the turnover of protein in a prototrophic strain of bacillus stearothermophilus during exponential growth in a salts medium with glucose or succinate as carbon source was about 4 %/h and in a richer nutrient broth medium about 23 %/h. protein degradation under non-growing conditions conformed to a similar pattern. the turnover of rna (non-messenger) was about 1 %/h in salts medium and about 9 %/h in nutrient broth. the turnover of protein and rna in the thermophile is thus moderate rather than m ... | 1975 | 1206375 |
| the primary structure of trnaphe from bacillus stearothermophilus. | | 1975 | 1227967 |
| subunit interactions in the methionyl-trna synthetase of bacillus stearothermophilus. | the methionyl-trna synthetase from bacillus stearothermophilus is shown to be a dimer of 2 x 82,000 with identical subunits. it exhibits negative cooperativity in substrate binding and "virtual" halt-of-the-sites reactivity. the enzyme binds only 1 mol of methionine in the absence of other ligands, but several methods show that 2 mol of methionyl adenylate are bound per enzyme dimer. however, one of these adenylates is formed 480 times faster than the other (k1 = 29 sec-1 and k2 = 0.06 sec-1). w ... | 1976 | 1247516 |
| the primary structure of trna2val from bacillus stearothermophilus. | | 1976 | 1248633 |
| thermostable polynucleotide phosphorylases from bacillus stearothermophilus and thermus aquaticus. | polynucleotide phosphorylase from bacillus stearothermophilus has been purified to homogeneity. polyacrylamide gel electrophoresis run under denaturing conditions indicates that the enzyme is a tetramer with subunits of apparent molecular weight 51,000 daltons. a partial purification of polynucleotide phosphorylase from thermus aquaticus has also been effected. the two enzymes show similar catalytic properties, which differ little from those of mesophilic polynucleotide phosphorylases. the use o ... | 1976 | 1250699 |
| altered phospholipid metabolism in a temperature-sensitive mutant of a thermophilic bacillus. | the phospholipid metabolism of a temperature-sensitive mutant of a thermophilic bacillus was studied after the shift from a permissive (58 degrees c) to a restrictive (65 degrees c) growth temperature. during the short period of growth of the mutant at 65 degrees c, the proportions of cardiolipin and its 3-acyl derivative (lyso-cardiolipin) increased, and the proportions of phosphatidylglycerol and phosphatidylethanolamine decreased on cell dry weight basis. in 32p incorporation and turnover exp ... | 1976 | 1252088 |
| arginyl-trna synthetase from bacillus stearothermophilus: subunit structure of enzyme. | | 1976 | 1253985 |
| effect of calcium and anaerobiosis on the thermostability of bacillus stearothermophilus. | bacillus stearothermophilus nca 2184 lost viability and subsequently released cytoplasmic components when suspended in 0.1 m tris(hydroxymethyl)aminomethane (tris) buffer (ph 7.2) and incubated at 60 degrees c. cell lysis was prevented by the addition of 10 mm cacl2 to the tris-buffer suspension. cells which were incubated under anaerobic conditions for 20 min in the growth medium before they were collected were stable in the tris-buffer suspension without added calcium. anaerobic incubation eff ... | 1976 | 1260540 |
| calcium uptake by bacillus stearothermophilus: a requirement for thermophilic growth. | | 1976 | 1261679 |
| characteristics of bacillus stearothermophilus mutants blocked in catabolic function. | with polyacrylamide disc gel electrophoresis and specific staining, it was demonstrated that one mutation involving the alcohol dehydrogenase of a double mutant of bacillus stearothermophilus 1503 apparently prevented enzyme synthesis, and another lesion in the same organism resulted in synthesis of an inactive form of aconitase. some properties of the double mutant and two fumarase mutants are discussed in relation to similar mutants derived from bacillus subtilis. | 1976 | 1262307 |
| thermal resistance of bacillus thermophilus spores suspended in parenteral solutions. | | 1976 | 1268431 |
| surface topography of the bacillus stearothermophilus ribosome. | the surface topography of the intact 70s ribosomes and free 30s and 50s subunits from bacillus stearothermophilus strain 2184 was investigated by lactoperoxidase-catalyzed iodination. two-dimensional polyacrylamide gel electrophoresis was employed to separate ribosomal proteins for analysis of their reactivity. free 50s subunits incorporated about 18% more 125i than did 50s subunits derived from 70s ribosomes, whereas, free 30s subunits and 30s subunits derived from 70s ribosomes incorporated si ... | 1976 | 1272247 |
| association of ribosomal subunits: studies on the binding of polyamines to 30s and 50s particles. | | 1976 | 1278439 |
| single amino acid substitutions can further increase the stability of a thermophilic l-lactate dehydrogenase. | lactate dehydrogenases are of considerable interest as stereospecific catalysts in the chemical preparation of enantiomerically pure alpha-hydroxyacid synthons. for such applications in synthetic organic chemistry it would be desirable to have enzymes which tolerate elevated temperatures for prolonged reaction times, to increase productivity and to extend their applicability to poor substrates. here, two examples are reported of significant thermostabilizations, induced by site-directed mutagene ... | 1992 | 1287656 |
| crystallization and preliminary crystallographic analysis of a thermostable mutant of kanamycin nucleotidyltransferase. | a thermostable mutant of kanamycin nucleotidyltransferase isolated by cloning and selection for kanamycin resistance in bacillus stearothermophilus at 70 degrees c has been crystallized in a form suitable for high-resolution diffraction analysis. this enzyme catalyzes nucleotidyl group transfer from nucleoside triphosphates such as atp to hydroxyl groups of various aminoglycosides, thus inactivating the antibiotic. the kanamycin nucleotidyltransferase gene, originally encoded on plasmid pub110 f ... | 1992 | 1315501 |
| purification and characterization of dna polymerases from bacillus species. | dna polymerases from bacillus stearothermophilus, bacillus caldotenax, and bacillus caldovelox were purified by chromatography on deae-cellulose, phosphocellulose, and heparin-sepharose and obtained in high yield. the enzyme preparations are free of exo- and endonuclease activities. additional purification steps, e.g., hydrophobic interaction chromatography and chromatography on a mono q column or sucrose density gradient centrifugation, are needed to obtain the enzymes in the form of homogeneou ... | 1992 | 1320608 |
| design of a specific phenyllactate dehydrogenase by peptide loop exchange on the bacillus stearothermophilus lactate dehydrogenase framework. | restriction sites were introduced into the gene for bacillus stearothermophilus lactate dehydrogenase which enabled a region of the gene to be excised which coded for a mobile surface loop of polypeptide (residues 98-110) which normally seals the active site vacuole from bulk solvent and is a major determinant of substrate specificity. oligonucleotide-overlap extension (using the polymerase chain reaction) was used to obtain double-stranded dna regions which coded for different length and sequen ... | 1992 | 1324721 |
| molecular cloning and mapping of phenol degradation genes from bacillus stearothermophilus fdtp-3 and their expression in escherichia coli. | two genes of the meta pathway of phenol degradation were cloned from a phenol-utilizing strain of bacillus stearothermophilus and were mapped by subcloning and by use of a tn5 insertion mutation. they code for phenol hydroxylase and catechol 2,3-dioxygenase, respectively. the gene encoding catechol 2,3-dioxygenase, which is more thermostable than catechol 2,3-dioxygenase encoded by the other gene, shares rather limited homology with that from pseudomonas putida. | 1992 | 1325141 |
| enzymatic synthesis of paps with an atp-regeneration system. | sulfate activating enzymes, atp sulfurylase and aps kinase, were newly isolated from a thermophile, bacillus stearothermophilus. adenosine 3'-phosphate 5'-phosphosulfate (paps) was synthesized by these enzymes. the reaction proceeded more efficiently when an atp-regeneration system, using acetate kinase, was coupled to the reaction system. | 1992 | 1337784 |
| cloning and characterization of a gene from bacillus stearothermophilus var. non-diastaticus encoding a glycerol dehydrogenase. | a 4.1-kb ecori fragment which includes the gene (glda) encoding a glycerol dehydrogenase (g1dh; ec 1.1.1.6; glycerol:nad oxidoreductase) from bacillus stearothermophilus var. non-diastaticus has been cloned by virtue of its ability to restore glycerol utilisation to escherichia coli glycerol kinase (glpk) and glycerol-3-phosphate dehydrogenase (glpd) mutants. sequencing suggests that the glda gene is likely to be monocistronic and encodes a protein of 39450 da. the deduced amino acid composition ... | 1992 | 1339360 |
| assay of gamma-glutamyltransferase with amino acid dehydrogenases from bacillus stearothermophilus as auxiliary enzymes. | a spectrophotometric assay for serum gamma-glutamyltransferase (gamma-gt, ec 2.3.2.2) based on the increasing absorbance at 340 nm due to nadh formation is described. using gamma-glutamyl dipeptides as the donor substrate, amino acids formed by the gamma-glutamyl transfer from the donor substrate to the acceptor substrate glycylglycine are stoichiometrically converted by the corresponding amino acid dehydrogenases from bacillus stearothermophilus to produce their keto acids and nadh. gamma-gluta ... | 1992 | 1350523 |
| effect of the weak ca(2+)-binding site of subtilisin j by site-directed mutagenesis on heat stability. | the functional role of the negatively charged amino acid residue in subtilisin j from bacillus stearothermophilus has been investigated by site-directed mutagenesis. glu-195 located at the weak ca2+-binding site was replaced with gln to examine the role of glu-195 in the heat stability of subtilisin j. mutant enzyme was expressed in bacillus subtilis and was purified from the culture supernatant. when the mutant enzyme was expressed at 37 degrees c in the presence of 2mm calcium chloride, the pa ... | 1992 | 1358066 |
| characterization and functional expression in escherichia coli of the sodium/proton/glutamate symport proteins of bacillus stearothermophilus and bacillus caldotenax. | the genes encoding the na+/h+/l-glutamate symport proteins of the thermophilic organisms bacillus stearothermophilus (glttbs) and bacillus caldotenax (glttbc) were cloned by complementation of escherichia coli jc5412 for growth on glutamate as sole source of carbon, energy and nitrogen. the nucleotide sequences of the glttbs and glttbc genes were determined. in both cases the translated sequences corresponded with proteins of 421 amino acid residues (96.7% amino acid identity between glttbs and ... | 1992 | 1359385 |
| the influence of chaperonins on protein folding. a mechanism for increasing the yield of the native form. | | 1992 | 1362048 |
| production of 5-methyluridine by immobilized thermostable purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from bacillus stearothermophilus jts 859. | 5-methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from bacillus stearothermophilus jts 859. the process was carried out in a column reactor at 60 degrees c for 17 d without any bacterial contamination under non-aseptical conditions. half-lives of the activity of the immobilized enzymes were 47 d and 4.5 d at 60 degrees c and 70 deg ... | 1991 | 1367490 |
| molecular cloning and nucleotide sequence determination of the bacillus stearothermophilus nca 1503 superoxide dismutase gene and its overexpression in escherichia coli. | the gene (sod) encoding bacillus stearothermophilus mn-superoxide dismutase (mnsod) has been cloned in escherichia coli and its entire nucleotide sequence determined. with the exception of the post-translationally cleaved n-terminal methionine residue, the predicted amino acid sequence exhibits complete identity to the previously determined amino acid sequence. the recombinant mnsod was shown to be functionally active in e. coli both in vitro and in vivo, and was expressed to 49% of the soluble ... | 1991 | 1367808 |
| physiology and performance of thermophilic microorganisms in sewage sludge treatment processes. | | 1990 | 1368146 |
| bacillus stearothermophilus glyceraldehyde-3-phosphate dehydrogenase as a source of angiotensin-converting enzyme inhibitors. | the more potent inhibitory activity against angiotensin-converting enzyme (ace) was excised from a glyceraldehyde-3-phosphate dehydrogenase (gapdh) preparation of bacillus stearothermophilus by heating at 120 degrees c in 1 m acoh-20 mm hcl, as compared with gapdh preparations of yeast and pig. sufficient excision of b. stearothermophilus ace inhibitors required a longer proteolysis time of 60 min. two inhibitors were then purified by gel-permeation and reverse-phase chromatographies. one of the ... | 1990 | 1368612 |
| the nucleotide sequences of bacillus stearothermophilus ribosomal protein s12 and s7 genes: comparison with the str operon of escherichia coli. | the primary structure of the ribosomal protein s7 from bacillus stearothermophilus (bsts7) was analyzed by a combination of amino acid and dna sequence analysis. peptide sequence information was derived from tryptic peptides of bsts7 by manual edman degradation. the nucleotide sequence of the 1.5-kb sali fragment from b. stearothermophilus genome, cloned by the escherichia coli s12 gene (rpsl) as a hybridization probe, was determined. comparison of deduced amino acid sequence with the correspond ... | 1991 | 1368664 |