| draft genome of brugia pahangi: high similarity between b. pahangi and b. malayi. | efforts to completely eradicate lymphatic filariasis from human population may be challenged by the emergence of brugia pahangi as another zoonotic lymphatic filarial nematode. in this report, a genomic study was conducted to understand this species at molecular level. | 2015 | 26350613 |
| transcriptomic analysis of musca domestica to reveal key genes of the prophenoloxidase-activating system. | the propo system regulates melanization in arthropods. however, the genes that are involved in the propo system in housefly musca domestica remain unclear. thus, this study analyzed the combined transcriptome obtained from m. domestica larvae, pupae, and adults that were either normal or bacteria-challenged by an escherichia coli and staphylococcus aureus mixture. a total of 54,821,138 clean reads (4.93 gb) were yielded by illumina sequencing, which were de novo assembled into 89,842 unigenes. o ... | 2015 | 26156588 |
| overexpression of tyrosine hydroxylase and dopa decarboxylase associated with pupal melanization in spodoptera exigua. | melanism has been found in a wide range of species, but the molecular mechanisms involved remain largely elusive. in this study, we studied the molecular mechanisms of the pupal melanism in spodoptera exigua. the full length cdna sequences of tyrosine hydroxylase (th) and dopa decarboxylase (ddc), two key enzymes in the biosynthesis pathway of melanin, were cloned, and their temporal expression patterns in the integument were compared during the larval-pupal metamorphosis process of the s. exigu ... | 2015 | 26084938 |
| key enzymes and proteins of crop insects as candidate for rnai based gene silencing. | rna interference (rnai) is a mechanism of homology dependent gene silencing present in plants and animals. it operates through 21-24 nucleotides small rnas which are processed through a set of core enzymatic machinery that involves dicer and argonaute proteins. in recent past, the technology has been well appreciated toward the control of plant pathogens and insects through suppression of key genes/proteins of infecting organisms. the genes encoding key enzymes/proteins with the great potential ... | 2015 | 25954206 |
| tissue-specific immune gene expression in the migratory locust, locusta migratoria. | the ability of hosts to respond to infection involves several complex immune recognition pathways. broadly conserved pathogen-associated molecular patterns (pamps) allow individuals to target a range of invading microbes. recently, studies on insect innate immunity have found evidence that a single pathogen can activate different immune pathways across species. in this study, expression changes in immune genes encoding peptidoglycan-recognition protein sa (pgrp-sa), gram-negative binding protein ... | 2015 | 26463191 |
| exome and transcriptome sequencing of aedes aegypti identifies a locus that confers resistance to brugia malayi and alters the immune response. | many mosquito species are naturally polymorphic for their abilities to transmit parasites, a feature which is of great interest for controlling vector-borne disease. aedes aegypti, the primary vector of dengue and yellow fever and a laboratory model for studying lymphatic filariasis, is genetically variable for its capacity to harbor the filarial nematode brugia malayi. the genome of ae. aegypti is large and repetitive, making genome resequencing difficult and expensive. we designed exome captur ... | 2015 | 25815506 |
| three-dimensional structure of a protozoal double-stranded rna virus that infects the enteric pathogen giardia lamblia. | giardia lamblia virus (glv) is a small, nonenveloped, nonsegmented double-stranded rna (dsrna) virus infecting giardia lamblia, the most common protozoan pathogen of the human intestine and a major agent of waterborne diarrheal disease worldwide. glv (genus giardiavirus) is a member of family totiviridae, along with several other groups of protozoal or fungal viruses, including leishmania rna viruses and trichomonas vaginalis viruses. interestingly, glv is more closely related than other totivir ... | 2014 | 25378500 |
| molecular cloning and mrna expression of the peptidoglycan recognition protein gene hcpgrp1 and its isoform hcpgrp1a from the freshwater mussel hyriopsis cumingi. | peptidoglycan recognition proteins (pgrps) are innate immune molecules that have been structurally conserved throughout evolution in invertebrates and vertebrates. in this study, peptidoglycan recognition protein hcpgrp1 and its isoform hcpgrp1a were identified in the freshwater mussel hyriopsis cumingii. the full-length cdnas of hcpgrp1 (973 bp) and hcpgrp1a (537 bp) encoded polypeptides with 218 and 151 amino acids, respectively. sequence analysis showed that hcpgrp1 had one c-terminal pgrp do ... | 0 | 25249773 |
| expression pattern of glycoside hydrolase genes in lutzomyia longipalpis reveals key enzymes involved in larval digestion. | the sand fly lutzomyia longipalpis is the most important vector of american visceral leishmaniasis. adults are phytophagous (males and females) or blood feeders (females only), and larvae feed on solid detritus. digestion in sand fly larvae has scarcely been studied, but some glycosidase activities putatively involved in microorganism digestion were already described. nevertheless, the molecular nature of these enzymes, as the corresponding genes and transcripts, were not explored yet. catabolis ... | 2014 | 25140153 |
| remarkable diversity of endogenous viruses in a crustacean genome. | recent studies in paleovirology have uncovered myriads of endogenous viral elements (eves) integrated in the genome of their eukaryotic hosts. these fragments result from endogenization, that is, integration of the viral genome into the host germline genome followed by vertical inheritance. so far, most studies have used a virus-centered approach, whereby endogenous copies of a particular group of viruses were searched in all available sequenced genomes. here, we follow a host-centered approach ... | 0 | 25084787 |
| mosquito hemocyte-mediated immune responses. | hemocytes are a key component of the mosquito immune system that kill pathogens via phagocytic, lytic and melanization pathways. individual mosquitoes contain between 500 and 4,000 hemocytes, which are divided into three populations named granulocytes, oenocytoids and prohemocytes. hemocytes can also be divided by their anatomical location with 75% of hemocytes circulating in the hemocoel (circulating hemocytes) and 25% of hemocytes attaching themselves to tissues (sessile hemocytes). greater th ... | 0 | 25309850 |
| in silico single strand melting curve: a new approach to identify nucleic acid polymorphisms in totiviridae. | the pcr technique and its variations have been increasingly used in the clinical laboratory and recent advances in this field generated new higher resolution techniques based on nucleic acid denaturation dynamics. the principle of these new molecular tools is based on the comparison of melting profiles, after denaturation of a dna double strand. until now, the secondary structure of single-stranded nucleic acids has not been exploited to develop identification systems based on pcr. to test the p ... | 2014 | 25030031 |
| insect prophenoloxidase: the view beyond immunity. | insect prophenoloxidase (ppo) is an important innate immunity protein due to its involvement in cellular and humoral defense. it belongs to a group of type-3 copper-containing proteins that occurs in almost all organisms. insect ppo has been studied for over a century, and the ppo activation cascade is becoming clearer. the insect ppo activation pathway incorporates several important proteins, including pattern-recognition receptors (pgrp, β grp, and c-type lectins), serine proteases, and serine ... | 2014 | 25071597 |
| mosquito larvicidal potential of four common medicinal plants of india. | mosquitoes transmit serious human health diseases, causing millions of deaths every year. plants may be sources of alternative mosquito control agents. the present study was carried out to assess the role of larvicidal activities of the crude extracts of four plants viz. alternanthera sessilis l. (amaranthaceae), trema orientalis l. (cannabaceae), gardenia carinata smith. (rubiaceae) and ruellia tuberosa l. (acanthaceae) against culex quinquefasciatus say in laboratory bioassay. | 0 | 25222784 |
| identification of immunity-related genes in ostrinia furnacalis against entomopathogenic fungi by rna-seq analysis. | the asian corn borer (ostrinia furnacalis (guenée)) is one of the most serious corn pests in asia. control of this pest with entomopathogenic fungus beauveria bassiana has been proposed. however, the molecular mechanisms involved in the interactions between o. furnacalis and b. bassiana are unclear, especially under the conditions that the genomic information of o. furnacalis is currently unavailable. so we sequenced and characterized the transcriptome of o. furnacalis larvae infected by b. bass ... | 2014 | 24466095 |
| phenylalanine metabolism regulates reproduction and parasite melanization in the malaria mosquito. | the blood meal of the female malaria mosquito is a pre-requisite to egg production and also represents the transmission route for the malaria parasite. the proper and rapid assimilation of proteins and nutrients in the blood meal creates a significant metabolic challenge for the mosquito. to better understand this process we generated a global profile of metabolite changes in response to blood meal of anopheles gambiae, using gas chromatography-mass spectrometry (gc-ms). to disrupt a key pathway ... | 2014 | 24409310 |
| identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean calanus finmarchicus. | neurochemicals are likely to play key roles in physiological/behavioral control in the copepod crustacean calanus finmarchicus, the biomass dominant zooplankton for much of the north atlantic ocean. previously, a de novo assembled transcriptome consisting of 206,041 unique sequences was used to characterize the peptidergic signaling systems of calanus. here, this assembly was mined for transcripts encoding enzymes involved in amine biosynthesis. using known drosophila melanogaster proteins as te ... | 2013 | 24148657 |
| the aquatic communities inhabiting internodes of two sympatric bamboos in argentinean subtropical forest. | in order to determine if phytotelmata in sympatric bamboos of the genus guadua might be colonized by different types of arthropods and contain communities of different complexities, the following objectives were formulated: (1) to analyze the structure and species richness of the aquatic macroinvertebrate communities, (2) to comparatively analyze co-occurrences; and (3) to identify the main predators. field studies were conducted in a subtropical forest in argentina, where 80 water-filled bamboo ... | 0 | 24224775 |
| mosquito-parasite interactions can shape filariasis transmission dynamics and impact elimination programs. | the relationship between mosquito vectors and lymphatic filariasis (lf) parasites can result in a range of transmission outcomes. anophelines are generally characterized as poor vectors due to an inability to support development at low densities. however, it is important to understand the potential for transmission in natural vectors to maximize the success of elimination efforts. primary vectors in papua new guinea (n = 1209) were dissected following exposure to microfilaremic blood (range 8-23 ... | 2013 | 24069488 |
| salivary glands proteins expression of anopheles dirus a fed on plasmodium vivax- and plasmodium falciparum-infected human blood. | mosquitoes are able to adapt to feed on blood by the salivary glands which created a protein that works against the haemostasis process. this study aims to investigate the salivary glands proteins expression of 50 adult female an. dirus a mosquitoes, a main vector of malaria in thailand, each group with an age of 5 days which were artificial membrane fed on sugar, normal blood, blood infected with p. vivax, and blood infected with p. falciparum. then mosquito salivary gland proteins were analyze ... | 2013 | 23956841 |
| non-genetic determinants of mosquito competence for malaria parasites. | understanding how mosquito vectors and malaria parasites interact is of fundamental interest, and it also offers novel perspectives for disease control. both the genetic and environmental contexts are known to affect the ability of mosquitoes to support malaria development and transmission, i.e., vector competence. although the role of environment has long been recognized, much work has focused on host and parasite genetic effects. however, the last few years have seen a surge of studies reveali ... | 2013 | 23818841 |
| complete genome sequence of a putative novel victorivirus from ustilaginoidea virens. | ustilaginoidea virens is the causal agent of a serious disease of rice. here, we report the presence of five dsrna bands ranging from about 1.2, 1.5, 1.7, and 1.8 to 5.6 kb in an isolate of this fungus from china and the complete sequence of the largest dsrna segment, putatively representing the genome of a novel virus, designated as ustilaginoidea virens rna virus 1 (uvrv1), uvrv1, which has a genome length of 5567 bp and has two consecutive open reading frames (orfs) with a five-nucleotide ove ... | 2013 | 23385326 |
| cdna cloning and expression analysis of pattern recognition proteins from the chinese oak silkmoth, antheraea pernyi. | pattern recognition receptors play an important role in insect immune defense. we cloned the β-1,3-glucan recognition protein, lectin-5 and c-type lectin 1 genes of antheraea pernyi and examined the expression profiles of immune-stimulated pupae. after infection with bacillus subtilis, escherichia coli, antheraea pernyi nuclear polyhedrosis virus (apnpv) and saccharomyces cerevisiae, respectively, the pupae showed different gene expression levels in the different tissues examined (midgut, fatbod ... | 2012 | 26466728 |
| surveillance and vector control of lymphatic filariasis in the republic of korea. | until the early 2000s, lymphatic filariasis would commonly break out in the coastal areas in korea. through steady efforts combining investigation and treatment, filariasis was officially declared eradicated in 2008. this study surveyed the density of vector species of filariasis in past endemic areas, and inspected filariasis dna from collected mosquitoes for protection against the reemergence of filariasis. | 0 | 24159506 |
| existence of prophenoloxidase in wing discs: a source of plasma prophenoloxidase in the silkworm, bombyx mori. | in insects, hemocytes are considered as the only source of plasma prophenoloxidase (ppo). ppo also exists in the hemocytes of the hematopoietic organ that is connected to the wing disc of bombyx mori. it is unknown whether there are other cells or tissues that can produce ppo and release it into the hemolymph besides circulating hemocytes. in this study, we use the silkworm as a model to explore this possibility. through tissue staining and biochemical assays, we found that wing discs contain pp ... | 2012 | 22848488 |
| tissue-enriched expression profiles in aedes aegypti identify hemocyte-specific transcriptome responses to infection. | hemocytes are integral components of mosquito immune mechanisms such as phagocytosis, melanization, and production of antimicrobial peptides. however, our understanding of hemocyte-specific molecular processes and their contribution to shaping the host immune response remains limited. to better understand the immunophysiological features distinctive of hemocytes, we conducted genome-wide analysis of hemocyte-enriched transcripts, and examined how tissue-enriched expression patterns change with t ... | 2012 | 22796331 |
| cloning and characterization of prophenoloxidase a3 (propoa3) from culex pipiens pallens. | the prophenoloxidase subunit a3 (propoa3) gene was cloned from culex pipiens pallens, which had an open reading frame of 2061 bp encoding a putative 686 amino acid protein. the deduced amino acid sequence shares 98% with propoa3 from culex quinquefasciatus. propoa3 is expressed at all developmental stages of c. pipiens pallens. significant negative correlation was observed between propoa3 expression and deltamethrin resistance in resistant c. pipiens pallens. furthermore, propoa3 expression leve ... | 2012 | 22561195 |
| filariasis of uncommon nature in india. | | 0 | 23509678 |
| intron retention in the drosophila melanogaster rieske iron sulphur protein gene generated a new protein. | genomes can encode a variety of proteins with unrelated architectures and activities. it is known that protein-coding genes of de novo origin have significantly contributed to this diversity. however, the molecular mechanisms and evolutionary processes behind these originations are still poorly understood. here we show that the last 102 codons of a novel gene, noble, assembled directly from non-coding dna following an intronic deletion that induced alternative intron retention at the drosophila ... | 0 | 21610726 |
| vector mosquitoes of filariasis in japan. | | 0 | 22028600 |
| brugian filariasis in japan. | | 0 | 22028598 |
| basic studies on filaria and filariasis. | | 0 | 22028602 |
| the primary role of fibrinogen-related proteins in invertebrates is defense, not coagulation. | in vertebrates, the conversion of fibrinogen into fibrin is an essential process that underlies the establishment of the supporting protein framework required for coagulation. in invertebrates, fibrinogen-domain-containing proteins play a role in the defense response generated against pathogens; however, they do not function in coagulation, suggesting that this role has been recently acquired. molecules containing fibrinogen motifs have been identified in numerous invertebrate organisms, and mos ... | 2010 | 21063081 |
| indoor resting density pattern of mosquito species in fingeswar block of raipur district in chhattisgarh, central india. | anopheline mosquitoes are vectors of human malaria and hence they are of greatest concern. around 40% of the world's population resides in malaria affected areas. malaria affects poor communities and causes enormous economic losses. the deadly disease annually causes clinical illness in 400-600 million people and kills 2-3 millions annually (who 2008). faunistic survey was conducted in fingeswar block in raipur district of chhattisgarh state of india during january 2003 to march 2004 to asses th ... | 2010 | 23129894 |
| phylogenetic analysis and temporal diversification of mosquitoes (diptera: culicidae) based on nuclear genes and morphology. | phylogenetic analyses provide a framework for examining the evolution of morphological and molecular diversity, interpreting patterns in biogeography, and achieving a stable classification. the generic and suprageneric relationships within mosquitoes (diptera: culicidae) are poorly resolved, making these subjects difficult to address. | 2009 | 20028549 |
| differential expression of aedes aegypti salivary transcriptome upon blood feeding. | saliva of aedes aegypti contains a complex array of proteins essential for both blood feeding and pathogen transmission. a large numbers of those proteins are classified as unknown in regard to their function(s). understanding the dynamic interactions at the mosquito-host interface can be achieved in part by characterizing mosquito salivary gland gene expression relative to blood feeding. towards this end, we developed an oligonucleotide microarray representing 463 transcripts to determine diffe ... | 2009 | 19630962 |
| transcription in mosquito hemocytes in response to pathogen exposure. | mosquito hemocytes are blood cells that are fundamental for combating systemic infection. a study published in bmc genomics shows that hemocyte gene transcription in response to immune challenge is pathogen-specific and reaffirms the primary role of these cells in immunity. | 2009 | 19519953 |
| molecular and functional analyses of amino acid decarboxylases involved in cuticle tanning in tribolium castaneum. | aspartate 1-decarboxylase (adc) and 3,4-dihydroxyphenylalanine decarboxylase (ddc) provide beta-alanine and dopamine used in insect cuticle tanning. beta-alanine is conjugated with dopamine to yield n-beta-alanyldopamine (nbad), a substrate for the phenol oxidase laccase that catalyzes the synthesis of cuticle protein cross-linking agents and pigment precursors. we identified adc and ddc genes in the red flour beetle, tribolium castaneum (tc), and investigated their functions. tcadc mrna was mos ... | 2009 | 19366687 |
| evolution and potential function of fibrinogen-like domains across twelve drosophila species. | the fibrinogen-like (fbg) domain consists of approximately 200 amino acid residues, which has high sequence similarity to the c-terminal halves of fibrinogen beta and gamma chains. fibrinogen-related proteins (freps) containing one or more fbg domains are found universally in vertebrates and invertebrates. in invertebrates, freps are involved in immune responses and other aspects of physiology. to understand the complexity of this gene family in drosophila, we analyzed freps in twelve drosophila ... | 2008 | 18513432 |
| grim-19: a double-edged sword that regulates anti-tumor and innate immune responses. | gene associated with retinoid-interferon-β-induced mortality (grim)-19, was originally identified as a critical regulatory protein necessary for interferon-β-retinoic acid-induced cell death. overexpression of grim-19 activates cell death and its suppression or inactivation promotes cell growth. grim-19 targets multiple proteins/pathways for exerting growth control and cell death. however, grim-19 is also required for normal cellular processes. in addition, viruses 'hijack' grim-19 for their sur ... | 2008 | 21566745 |
| the juan non-ltr retrotransposon in mosquitoes: genomic impact, vertical transmission and indications of recent and widespread activity. | in contrast to dna-mediated transposable elements (tes), retrotransposons, particularly non-long terminal repeat retrotransposons (non-ltrs), are generally considered to have a much lower propensity towards horizontal transfer. detailed studies on site-specific non-ltr families have demonstrated strict vertical transmission. more studies are needed with non-site-specific non-ltr families to determine whether strict vertical transmission is a phenomenon related to site specificity or a more gener ... | 2007 | 17620143 |
| identification and molecular characterization of a prophenoloxidase involved in aedes aegypti chorion melanization. | chorion melanization is a vital biochemical event for the survival of mosquito eggs in the environment. this study describes the identification and molecular characterization of a prophenoloxidase (propo) involved in chorion melanization in aedes aegypti by various biochemical and molecular techniques. results revealed that transcription of the chorion propo occurs only in adults, blood feeding greatly stimulated its transcription and haemocytes are responsible for its transcription. our study p ... | 0 | 15796752 |
| effects of elevated atmospheric co(2) on water chemistry and mosquito (diptera: culicidae) growth under competitive conditions in container habitats. | we investigated the direct and indirect effects of elevated atmospheric co(2) on freshwater container habitats and their larval mosquito occupants. we predicted that a doubling of atmospheric co(2) would (1) alter the chemical properties of water in this system, (2) slow degradation of leaf litter, and (3) decrease larval growth of aedes albopictus (skuse) mosquitoes raised on that litter under competitive conditions. effects of elevated co(2) on water quality parameters were not detected, but t ... | 0 | 22661767 |
| description of the transcriptomes of immune response-activated hemocytes from the mosquito vectors aedes aegypti and armigeres subalbatus. | mosquito-borne diseases, including dengue, malaria, and lymphatic filariasis, exact a devastating toll on global health and economics, killing or debilitating millions every year (54). mosquito innate immune responses are at the forefront of concerted research efforts aimed at defining potential target genes that could be manipulated to engineer pathogen resistance in vector populations. we aimed to describe the pivotal role that circulating blood cells (called hemocytes) play in immunity by gen ... | 0 | 15213157 |
| the colonization and laboratory maintenance of armigeres subalbatus (coquillett). | | 0 | 14272435 |
| antibacterial peptide cecropinb2 production via various host and construct systems. | cecropin is a cationic antibacterial peptide composed of 35-39 residues. this peptide has been identified as possessing strong antibacterial activity and low toxicity against eukaryotic cells, and it has been claimed that some types of the cecropin family of peptides are capable of killing cancer cells. in this study, the host effect of cloning antibacterial peptide cecropinb2 was investigated. three different host expression systems were chosen, i.e., escherichia coli, bacillus subtilis and pic ... | 2016 | 26784164 |
| vector and reservoir host of a case of human brugia pahangi infection in selangor, peninsular malaysia. | a case of human eye infection caused by brugia pahangi was reported in 2010 in a semi rural village in selangor, peninsular malaysia. our report here reveals results of investigation on the vector and animal host for the transmission of the infection. we conducted entomological survey and cat blood examination in the vicinity of the patient's home. the mosquito species armigeres subalbatus was incriminated as the vector, whereas cat served as the reservoir host. | 2013 | 24522144 |
| armigeres subalbatus colonization of damaged pit latrines: a nuisance and potential health risk to residents of resettlement villages in laos. | during the resettlement of 6500 persons living around the nam theun 2 hydroelectric project in laos, more than 1200 pour-flush latrines were constructed. to assess the role of these latrines as productive larval habitats for mosquitoes, entomological investigations using centers for disease control (cdc) light traps, visual inspection and emergence trapping were carried out in over 300 latrines during the rainy seasons of 2008-2010. armigeres subalbatus (diptera: culicidae) were nine times more ... | 2016 | 26508317 |
| establishment and characterization of two new cell lines from the mosquito armigeres subalbatus (coquillett) (diptera: culicidae). | armigeres subalbatus (coquillett) is a medically important mosquito and a model species for immunology research. we successfully established two cell lines from the neonate larvae of a. subalbatus using two different media. to our knowledge, this is the first report of an established armigeres mosquito cell line. the cell lines, designated as ar-3 and ar-13, consist of adherent and diploid cells with compact colonies. both these cell lines grow slowly after passage at a split ratio of 1:5 and a ... | 2015 | 25761724 |
| insights into the different functions of multiple peptidoglycan recognition proteins in the immune response against bacteria in the mosquito, armigeres subalbatus. | peptidoglycan recognition proteins (pgrps) are a group of proteins that recognize and/or bind to peptidoglycan on the surface of a number of pathogens. to understand the roles of multiple pgrps in the mosquito armigeres subalbatus (aspgrps), we studied the effects of infection of two bacteria, the gram negative escherichia coli and the gram positive micrococcus luteus, on the transcriptional expression of aspgrps and rna interference (rnai) of aspgrps on the immune responses of mosquitoes agains ... | 2013 | 23541606 |
| [isolation and identification of mosquito-borne arboviruses in yuncheng city, shanxi province, 2012]. | to investigate the species and distribution of mosquitoes and mosquito-borne arboviruses in yuncheng city of shanxi province, china. | 2015 | 25975552 |
| distribution of mosquitoes and mosquito-borne viruses along the china-myanmar border in yunnan province. | a total of 54,673 mosquitoes were collected at 11 sites located near the china-myanmar border in the western part of yunnan province during july and august 2007. there were 29 species in 4 genera identified from the collections, including 12 species of culex, 12 species of anopheles, 3 species of aedes, and 2 species of armigeres. culex tritaeniorhynchus giles (67.9%, 37,119/54,673) and anopheles sinensis wiedemann (25.9%, 14,170/54,673) were the most abundant species in this investigation. viru ... | 2012 | 22627302 |
| application of biogenic carbon dioxide produced by yeast with different carbon sources for attraction of mosquitoes towards adult mosquito traps. | surveillance is a prime requisite for controlling arthropod vectors like mosquitoes that transmit diseases such as malaria, dengue and chikungunya. carbon dioxide (co2) is one of the main cues from vertebrate breath that attracts mosquitoes towards the host. hence, co2 is used as an attractant during surveillance of mosquitoes either from commercial cylinders or dry ice for mosquito traps. in the present study, the biogenic carbon dioxide production was optimized with different carbon sources su ... | 2016 | 26677098 |
| seasonal prevalence and blood meal analysis of filarial vector culex quinquefasciatus in coastal areas of digha, west bengal, india. | filariasis is one of the major vector-borne diseases causing serious health problem in the tropics and subtropics. the coastal areas of digha are known to be a filariasis prone region of west bengal, india. the filarial parasite wuchereria bancrofti is transmitted by culex quinquefasciatus, the established filarial vector in west bengal, india. the present work was aimed to determine the abundance of different mosquito species; and the frequency, distribution and blood meal analysis of cx. quinq ... | 2015 | 26418657 |
| evaluation of larvicidal activity of medicinal plant extracts against three mosquito vectors. | to evaluate the mosquito larvicidal activity of plant extracts. | 2011 | 21771411 |
| comparison of the morphology of oocysts and the phylogenetic analysis of four ascogregarina species (eugregarinidae: lecudinidae) as inferred from small subunit ribosomal dna sequences. | this study on the ultrastructure of the oocysts of four isolated species of ascogregarina (a. taiwanensis (lien and levine) (eugregarinidae: lecudinidae) from aedes albopictus (skuse), a. culicis (ross) (eugregarinidae: lecudinidae) from aedes aegypti (l.), a. armigerei (eugregarinidae: lecudinidae) from armigeres subalbatus (coquillet), and ascogregarina sp. (eugregarinidae: lecudinidae) from ochlerotatus japonicus japonicus (theobald)) using a scanning electron microscope revealed significant ... | 2007 | 17291827 |
| beta-cyfluthrin, a synthetic pyrethroid for mosquito control. | beta-cyfluthrin (oms 3051), a new synthetic pyrethroid and one of the stereoisomers of cyfluthrin, was studied for insecticidal activity against eight mosquito species. its larvicidal activity with lc50 values of 5.62 x 10(-5) and 1.19 x 10(-4) mg/l respectively for culex quinquefasciatus and aedes aegypti was comparable with that of deltamethrin. this pyrethroid was more effective against the larvae of armigeres subalbatus (lc50 - 7.76 x 10(-7)) and the adults of anopheles culicifacies lt50 - 2 ... | 1992 | 1359650 |
| tralomethrin (oms-3048), a new synthetic pyrethroid against vector mosquitoes. | tralomethrin (oms-3048) a synthetic pyrethroid, was tested for its insecticidal properties in the laboratory against normal strains of culex quinquefasciatus, cx. tritaeniorhynchus, cx. sitiens, anopheles stephensi, an. culicifacies, aedes aegypti and armigeres subalbatus and against strains of cx. quinquefasciatus resistant to fenthion and malathion. tralomethrin showed good larvicidal activity against all the species tested. lc50 ranged between 7.00 x 10(-6) and 9.10 x 10(-3) mg (ai)/l. resist ... | 1991 | 1688021 |
| evaluation of the control of mosquitoes with insect growth regulators. | in this study, the effectiveness of eight insect growth regulators (igrs) (chlorfluazuron, diflubenzuron, el-494, flufenoxuron, teflubenzuron, juglone, plumbagin and methoprene) against five mosquito vectors (armigeres subalbatus, aedes albopictus, aedes aegypti, culex tritaeniorhynchus, and culex quinquefasciatus) was investigated in the laboratory. the ec50s of chlorfluazuron, diflubenzuron, el-494, flufenoxuron, teflubenzuron, and methoprene against the five mosquitoes ranged from 0.0001 to 0 ... | 1990 | 2402025 |
| mosquito species (diptera: culicidae) persistence and synchrony across an urban altitudinal gradient. | patterns of mosquito spatial persistence and temporal presence, as well as synchrony, i.e., the degree of concerted fluctuations in abundance, have been scarcely studied at finely grained spatial scales and over altitudinal gradients. here, we present a spatial persistence, temporal presence, and synchrony analysis of four common mosquito species across the altitudinal gradient of mt. konpira in nagasaki, japan. we found that aedes albopictus (skuse) was more frequently found at the mountain bas ... | 2017 | 28025246 |
| temporal changes of aedes and armigeres populations in suburban and forested areas in malaysia. | surveillance of mosquitoes and their distribution in association with rainfall, relative humidity, and temperature were conducted in selected suburban and forested areas, namely, sungai penchala (kuala lumpur) and taman alam (selangor) for 12 months. armigeres kesseli was the most abundant species in sungai penchala while aedes butleri was the most dominant species in taman alam. a positive correlation between mosquito distribution and rainfall was observed in selected mosquito species in sungai ... | 2015 | 26867376 |
| mosquito biodiversity patterns around urban environments in south-central okinawa island, japan. | okinawa is the largest, most urbanized, and densely populated island in the ryukyus archipelago, where mosquito species diversity has been thoroughly studied. however, the south-central okinawa mosquito fauna has been relatively poorly studied. here, we present results from a mosquito faunal survey in urban environments of nishihara city, south-central okinawa. mosquitoes were sampled biweekly, from april 2007 to march 2008, at 3 different environments: a forest preserve, an animal farm, and a w ... | 2014 | 25843131 |
| distribution and mosquito hosts of chaoyang virus, a newly reported flavivirus from the republic of korea, 2008-2011. | in total, 183,602 female culicine mosquitoes were captured by mosquito magnet, black light, and new jersey light traps, and manual aspiration of resting blood-fed mosquitoes, in the republic of korea from 2008 to 2011. culicine mosquitoes were identified to species, placed in pools of up to 30 mosquitoes each, and screened for flavivirus rna by using an sybr green i-based reverse transcription-polymerase chain reaction assay. thirty-two of the 8,199 pools assayed were positive by quantitative po ... | 2014 | 24724298 |
| physicochemical characteristics of habitats in relation to the density of container-breeding mosquitoes in asom, india. | container-breeding mosquitoes, especially aedes spp are vectors of diseases such as dengue and chikungunya. the abundance of these disease vectors in an area depends on the availability of container habitats and their physicochemical characteristics. the species composition of container-breeding mosquitoes in asom, india was studied and the larval density was correlated with the habitat characteristics. | 2013 | 24220081 |
| a laboratory study on the life cycle and feeding behaviour of arrenurus madaraszi (acari: arrenuridae) parasitizing anopheles mosquitoes. | although mosquitoes of several genera were collected from one locality in thiruvananthapuram, kerala state, india, only anopheles spp. carried larval arrenurus madaraszi; 21.5% of the an. hyrcanus and 19.0% of the an. vagus were infested. however, the whole life cycle of arr. madaraszi could be replicated in the laboratory using aedes albopictus, ae. vittatus or armigeres subalbatus as foster hosts. the host specificity observed in the field is therefore ecological, not physiological. in the lab ... | 1994 | 7915101 |
| comparison of the effectiveness of two deet formulations against aedes albopictus in the philippines. | the effectiveness of 2 formulations of deet was tested in the philippines against aedes albopictus. a new military issue extended duration repellent formulation (edrf) was compared with the older standard liquid formulation. consistently fewer mosquitoes landed on subjects treated with the edrf than on those treated with the liquid formulation for up to 12 h post-application. however, the difference between the 2 formulations was not statistically significant. both treatments were significantly ... | 1991 | 1686272 |
| insecticidal activity of some new synthetic compounds against different mosquito species. | insecticidal activity of an organosilane (hoe 84498, oms 3055), organophosphorous (mat 9460, oms 3052) and a synthetic pyrethroid compound (trebon, oms 3002) was evaluated against adult and larval stages of eight species of mosquitoes. insecticidal activity of these compounds was limited to larval stages only. highest activity of hoe 84498 was found against both cx. tritaeniorhynchus and anopheles culicifacies (lc50-0.0065 mg/i), mat 9460 against armigeres subalbatus (lc50-0.00043 mg/i) and treb ... | 1995 | 9163708 |
| host feeding patterns of mosquitoes in a rural malaria-endemic region in hainan island, china. | malaria is endemic in wangxia village of hainan island. in this area little is known about the host seeking behavior and feeding habit of mosquitoes. three sites representing the most common habitat types in the village were selected to study the host seeking behavior and feeding habit of mosquitoes. of the total 9 species belonging to 4 genera (armigeres, culex, aedes, and anopheles) collected in wangxia village, culex tritaeniorhynchus and cx. pipiens quinquefasciatus were the most commonly co ... | 2014 | 25843138 |
| molecular detection of a novel totivirus from golden shiner (notemigonus crysoleucas) baitfish in the usa. | during regulatory and routine surveillance sampling of apparently healthy baitfish from the state of minnesota, a novel totivirus (tentatively named "golden shiner totivirus", gstv) was detected in a homogenate of kidney and spleen of golden shiner (notemigonus crysoleucas). the nearly complete genome is 7788 nt long with a complete 5' untranslated region (utr) of 135 nt (1-135 nt position), complete open reading frames (orfs) and a partial 3' utr of 54 nt (7734-7788). the sequence is comprised ... | 2016 | 27231008 |
| new insights about orf1 coding regions support the proposition of a new genus comprising arthropod viruses in the family totiviridae. | analyzing the positions of 2a-like polypeptide cleavage sites in all available genomes of arthropod totiviruses we propose the limits of all orf1 coding sequences and observed that two proteins previously predicted in infectious myonecrosis virus genome are unique in the arthropod totiviruses group. a putative protein cleavage site upstream the major capsid protein was also identified only in these genomes. in addition, protein models generated using ab initio and threading approaches revealed c ... | 2016 | 26497779 |
| the dual roles of armigeres subalbatus prophenoloxidase v in parasite melanization and egg chorion melanization in the mosquito ar. subalbatus. | phenoloxidases (pos) play key roles in various physiological functions in insects, e.g., cuticular sclerotization, wound healing, egg tanning, cuticle formation and melanotic encapsulaction of pathogens. previously, we identified five pos, designated as-pro-po i-v, from the mosquito armigeres subalbatus and demonstrated that the functions of as-pro-po i, ii and iii, were associated with filarial parasite melanization, blood feeding and cuticle formation, respectively. in the present study, we de ... | 2015 | 26226650 |
| population dynamics of armigeres subalbatus (diptera: culicidae) across a temperate altitudinal gradient. | understanding the impacts of weather fluctuations, and environmental gradients, on the abundance of vectors is fundamental to grasp the dynamic nature of the entomological risk for disease transmission. the mosquito armigeres subalbatus (coquillet) is a common vector of filariasis. nevertheless, its population dynamics have been relatively poorly studied. here, we present results from a season long study where we studied spatio-temporal abundance patterns of ar. subalbatus across the altitudinal ... | 2015 | 26074401 |
| identification and functional analysis of the peptidoglycan recognition protein ld gene in the mosquito, armigeres subalbatus. | peptidoglycan recognition proteins are important recognition proteins in many organisms ranging from echinoderms to humans. in an attempt to characterize all the pgrps in the mosquito armigeres subalbatus, two pgrp-ld isoforms, aspgrp-lda and aspgrp-ldb, which are orthologs of the pgrp-lds in several other insect species, were identified from this mosquito using homologous cloning. to date the functions of this pgrp gene have not yet been described in detail in other organisms with a known pgrp- ... | 2014 | 24016699 |
| cloning and characterization of the peptidoglycan recognition protein genes in the mosquito, armigeres subalbatus (diptera: culicidae). | peptidoglycan recognition proteins (pgrps) are a group of proteins that are responsible for the recognition and, in some cases, binding of peptidoglycan (pgn), a unique cell wall component of bacteria, and initiation of immune responses to various types of pathogens. in the current study, full-length cdna sequences of multiple pgrps, identified via a database search, were cloned in the mosquito armigeres subalbatus (coquillett). during cloning, a novel transcript variant (isoform) of aspgrp-lc ( ... | 2012 | 22679875 |
| armigeres subalbatus (diptera: culicidae) prophenoloxidase iii is required for mosquito cuticle formation: ultrastructural study on dsrna-knockdown mosquitoes. | we previously suggested that armigeres subalbatus (coquillett) prophenoloxidase iii (as-pro-po iii) might be associated with morphogenesis of larvae and pupae. because po and its activation system are present in the insect cuticle, and cuticle formation is a major event during pupal morphogenesis, we used ultrastructural analysis to examine the effects of as-pro-po iii knockdown on the formation of pupal and adult cuticle. inoculation of as-pro-po iii dsrna resulted in the incomplete formation o ... | 2010 | 20695265 |
| predatory efficiency of the sewage drain inhabiting larvae of toxorhynchites splendens wiedemann on culex quinquefasciatus say and armigeres subalbatus (coquillett) larvae. | the rate of predation by stage iv instar toxorhynchites splendens larvae on the equivalent instar stage larvae of culex quinquefasciatus and armigeres subalbatus, co-occurring in sewage drains, were noted for a period of three consecutive days in the laboratory using different prey densities and combinations. the rate of predation varied by age of the predator, density of prey, and prey type. the number of ar. subalbatus larvae consumed by a single tx. splendens larva ranged between 0.50 +/- 0.7 ... | 2007 | 18041295 |
| analysis of salivary gland proteins of the mosquito armigeres subalbatus. | quantitative studies of total salivary gland protein of armigeres subalbatus mosquito revealed that the total salivary gland protein increased dramatically during the five days after emergence as adults. the amount of salivary gland protein of female and male mosquitos at day five after adult emergence were on the average 11.55 and 1.32 microg/pair gland respectively. sds-page studies showed that salivary gland protein profiles of armigeres subalbatus demonstrated 9 major polypeptide bands of 68 ... | 2005 | 15906643 |
| electron microscopic observations on wound-healing in larvae of the mosquito armigeres subalbatus (diptera: culicidae). | the wound-healing processes in the mosquito armigeres subalbatus (coquillett) were observed with electron microscopy. the initial reaction involved wound contractions and aggregation of injured surface tissues, cell debris and movement of granulocytes toward the wound. granulocytes first aggregated around the surface of the wound and many filamentous filopodia protruded to connect with cytoplasmic strands. these strands were then interconnected to form a network coagulum resulting in wound closu ... | 2001 | 11761382 |
| molecular cloning of a second prophenoloxidase cdna from the mosquito armigeres subalbatus: prophenoloxidase expression in blood-fed and microfilariae-inoculated mosquitoes. | melanization constitutes an important component in various aspects of insect life, including cuticular sclerotization, egg-shell tanning, melanization of parasites and wound healing. recently, a cdna encoding prophenoloxidase (pro-po), a key enzyme in the biosynthesis of melanotic material in insects, was cloned from microfilariae (mf)-inoculated mosquitoes, armigeres subalbatus. however, results of northern blot analyses indicated that two pro-pos might be present in ar. subalbatus and these pr ... | 2001 | 11240640 |
| field observations on the swarming behavior of armigeres subalbatus (coq) (diptera : culicidae). | swarming behavior of armigeres subalbatus was observed in pondicherry, south india. swarming occurred both at dawn and dusk over stationary markers. the duration of swarming was longer at dust (135 minutes) and shorter at dawn (75 minutes). higher intensity in terms of relative density of participating males was observed at dusk when compared to dawn. peak swarming activity was observed at light intensity ranging between 10 and 183 lux. response to low light intensity of 1-10 lux was different d ... | 1995 | 8525406 |
| circadian rhythm in the biting behaviour of a mosquito armigeres subalbatus (coquillett). | the crepuscular biting rhythm of a. subalbatus has been found to be a genuine circadian rhythm. when the entrained biting rhythm is allowed to freerun in constant darkness (dd) and continuous illumination (ll), it persists in dd (tau = 24.36 hr) and also in ll of ca. 0.1 1x (tau-23.82 hr) thus deviating from the strict 24 hr periodicity of the geophysical day. the biting rhythm becomes arrhythmic in ll of 1.0, 10, 0.4, 4, 40 lx even the first cycle damping away. | 1994 | 7916332 |
| cannibalistic behavior in armigeres subalbatus (diptera: culicidae). | phenomenon of natural cannibalism was observed to be exhibited by late (iii and iv) instar larvae of armigeres subalbatus. cannibalistic behavior in this species was studied in response to food and density. cannibalism among late instars was found to occur even in the presence of an adequate quantity of food. the rate of cannibalism was enhanced when food was restricted to only the early stages. even in the total absence of food early instars did not show any cannibalistic behavior. density had ... | 1992 | 1362627 |
| inheritance of orange pupa and phosphoglucomutase in the mosquito armigeres subalbatus. | | 1987 | 2904972 |
| laboratory studies on the biology of armigeres subalbatus coquillett, 1898. | | 1981 | 6116668 |
| rhythms in the biting behaviour of a mosquito armigeres subalbatus. | the biting cycle of armigeres subalbatus is distinctly crepuscular, exhibiting two peaks of activity, a smaller one at dawn and a larger one at dusk. the biting cycle is entrained to natural light-dark cycles and the time interval from dawn to dawn or dusk to dusk peaks is exactly 24 h and from dawn to dusk or dusk to dawn is about 12 h measured at 50% level. this rhythm manifests itself day after day without any marked qualitative change.the rate of change of light intensity may determine the o ... | 1980 | 28309633 |
| relation between diel activity and respiration in armigeres subalbatus (coquillett) (diptera: culicidae). | | 1975 | 498 |
| double infection in a mosquito armigeres subalbatus (coquillett) (a. obturbans auct.) with two different species of filaria. | | 1970 | 4392055 |
| notes on the colonization and biology of armigeres subalbatus (diptera, culicidae). | | 1964 | 14183103 |
| identification of a newly isolated getah virus in the china-laos border, china. | in this study, we isolated a virus strain (yn12031) from specimens of armigeres subalbatus collected in the china-laos border. bhk-21 cells infected with yn12031 exhibited an evident cytopathic effect (cpe) 32 h post-infection. the virus particles were spherical, 70 nm in diameter, and enveloped; they also featured surface fibers. molecular genetic analysis revealed that yn12031 was closely related to alpha viruses such as chikungunya virus and sindbis virus, and located in the same clade as mm2 ... | 2017 | 28427491 |
| distribution and phylogeny of wolbachia strains in wild mosquito populations in sri lanka. | wolbachia are a group of maternally inherited intracellular bacteria known to be widespread among arthropods. infections with wolbachia cause declines of host populations, and also induce host resistance to a wide range of pathogens. over the past few decades, researchers were curious to use wolbachia as a biological tool to control mosquito vectors. during the present study, assessment of the prevalence of wolbachia infections among wild mosquito populations in sri lanka where mosquito-borne di ... | 2017 | 28490339 |
| pigsties near dwellings as a potential risk factor for the prevalence of japanese encephalitis virus in adult in shanxi, china. | the increasing trend of adult cases of japanese encephalitis (je) in china, particularly in northern china, has become an important public health issue. we conducted an epidemiological investigation in the south of shanxi province to examine the relationships between mosquitoes, japanese encephalitis virus (jev), and adult je cases. | 2017 | 28592296 |
| comparative metagenomic profiling of viromes associated with four common mosquito species in china. | vast viruses are thought to be associated with mosquitoes. anopheles sinensis, armigeres subalbatus, culex quinquefasciatus, and culex tritaeniorhynchus are very common mosquito species in china, and whether the virome structure in each species is species-specific has not been evaluated. in this study, a total of 2222 mosquitoes were collected from the same geographic location, and rnas were sequenced using the illumina miseq platform. after querying to the refseq database, a total of 3,435,781, ... | 2018 | 29500689 |
| molecular epidemiology and genetic diversity of zika virus from field-caught mosquitoes in various regions of thailand. | zika virus (zikv) infection is an emerging and re-emerging arbovirus disease that is transmitted to humans through the bite of infected mosquitoes. zikv infections were first described in thailand in 1954 from the sera of indigenous residents and several travelers returning from thailand in 2014. however, reported cases in thailand have been increasing since 2015 and 2016, and epidemiological information about the vectors of zikv is unclear. we investigated the molecular epidemiology and genetic ... | 2019 | 30845707 |
| molecular detection of rickettsia felis and rickettsia bellii in mosquitoes. | to add to the limited information on rickettsia in mosquitoes in china, we carried out a pcr survey on convenience samples of 3051 mosquitoes collected with hand nets in and around domestic dwellings in 25 provinces. five species of mosquitoes were identified: culex pipiens pallens (n = 1620), aedes albopictus (806), armigeres subalbatus (377), anopheles sinensis (168), and culex tritaeniorhynchus (80). a rickettsia nested-pcr targeting the variable domain of glta showed rickettsia felis in four ... | 2019 | 31306085 |
| [study on kdr allele mutation of culex pipiens pallens in zichuan district, zibo city, shandong province]. | to study the seasonal dynamics of culex pipiens pallens and the distribution of knockdown resistance (kdr) gene related sodium channel gene polymorphism in zichuan district, zibo city, shandong province. | 2019 | 31184042 |
| isolation and genome phylogenetic analysis of arthropod-borne viruses, including akabane virus, from mosquitoes collected in hunan province, china. | this study investigated the abundance of mosquitoes and circulation of mosquito-borne arboviruses from 16 villages in 8 cities of hunan province, china, in july-august of 2010 and in august of 2011. in total, 16,076 mosquitoes consisting of seven species from four genera were collected by ultraviolet-light trap. culex quinquefasciatus was the most common species, accounting for 50.63% (8140/16,076) of the total. anopheles sinensis (24.26%, 3900/16,076) made up the second most common species, fol ... | 2019 | 30526411 |
| [analysis of population genetic diversity of mosquitoes from shandong province based on mitochondrial dna cytochrome oxidase subunit i gene fragment]. | to explore the characteristics of gene sequence of mtdna-coⅰ of culex pipiens pallens from different geographical regions in shandong province and different resistant strains from the lab and five common mosquito species, and analyze the genetic diversity of these mosquitoes. | 2017 | 29536705 |
| prevalence of disease vectors in lakshadweep islands during post-monsoon season. | : increase of vector-borne diseases (vbds) in india has posed a question on the situation in lakshadweep islands, where vbds are reported from time-to-time. the present investigation was aimed to assess the faunastic situation of the prevailing vectors along with their breeding sites in different islands of the lakshadweep. | 2019 | 30618444 |
| [mosquitoes, midges and related arboviruses in southeast sichuan province]. | objective: to investigate the distribution patterns of mosquitoes, midges and related arboviruses in sichuan province. methods: blood-sucking insects were collected from houses and pens, using the ultraviolet lights. mosquito samples were classified according to morphologic characteristics and then stored at liquid nitrogen. all samples were incubated with bhk-21 and c6/36 cells for virus isolation and then detected for their viral genes. sequences of the virus were identified and analyzed by mo ... | 2018 | 30453441 |
| japanese encephalitis in indonesia: an update on epidemiology and transmission ecology. | the japanese encephalitis (je) virus circulation in indonesia was first documented in lombok in 1960, and the virus was first isolated in 1972 from culex tritaeniorhynchus in bekasi, west java and kapuk, west jakarta. since then, indonesia has been recognized as an endemic country for je transmission. up to now, je cases have been found in at least 29 provinces, with bali, west kalimantan, east nusa tenggara, west java and east java, being the areas of highest incidence. however, routine surveil ... | 2018 | 30118700 |