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inhibitory effects of citrus extracts on the experimental pulmonary fibrosis.a chinese herbal formula, hu-qi-yin possessed an anti-pulmonary fibrosis effect. pericarp of citrus reticulata, one of the herbal drugs contained in this formula showed the most potent inhibitory activity on the proliferation of human embryonic lung fibroblasts (helf). the present study was designed to clarify the active principles responsible for the activity and further explore the anti-pulmonary fibrosis effect in vivo.200919643175
phytochemical investigation of the fruit peels of citrus reticulata blanco.phytochemical investigation of the fruit peels of citrus reticulata blanco (rutaceae) resulted in the isolation of three new phytoconstituents along with n-hexacosonoic acid. their structures have been established as 18betah-urs-5,11-dien-3beta-ol-11-one-3beta-d-glucopyranosyl-(4'-->1'')-d-glucopyranosyl-6''-(3''',4'''-dihydroxyl)-benzoate (reticulataursenoside), stigmast-5-en-3beta-ol-3beta-d-glucopyranosyl-4'-eicosanoate (citrusteryl arachidate), and lanost-5-en-3beta-ol-3beta-d-glucopyranosyl ...201020401792
comparative analysis of volatile constituents in citrus reticulata blanco using gc-ms and alternative moving window factor analysis.the similarities and differences of essential oil components in album citri reticulatae (acr), cylindricae citri reticulatae (ccr), folium citri reticulatae (fcr), exocarpium citri grandis (ecg), pericarpium citri reticulatae viride (pcrv) and pericarpium citri reticulatae (pcr) were investigated by gc-ms combined with a chemometric method named alternative moving window factor analysis (amwfa). and temperature-programmed retention indices (ptris) were used together with mass spectra for identif ...200919750500
application of lc-nmr to analysis of carotenoids in foods.lc-nmr has been applied to componential analysis of carotenoids in several foods, specifically, tomato juice, palm oil, and satsuma mandarin orange juice. the crude carotenoids extracted with organic solvent from these foodstuffs were analyzed after simple pre-processing. three, four, and two kinds of carotenoids were identified for tomato juice, palm oil, and satsuma mandarin orange, respectively, primarily by comparing their nmr spectra with those of pure standard.200919810055
clinical effectiveness of kss formula, a traditional folk remedy for alcohol hangover symptoms.a formula (kss formula) containing the pith of citrus tangerine hort. et tanaka (kitsuraku), the rhizome of zingiber officinale (shokyo), and brown sugar has been traditionally used in china for the treatment of discomfort and cold sensation in the abdomen after ingestion of large amounts of alcohol. we evaluated the clinical effectiveness of this formula on signs and symptoms of alcohol hangover (ah). of the twenty-two symptoms listed, significant decreases in severity scores were shown in naus ...201020559749
[study on the contents of flavonoids in citrus reticulata 'chachi' from various habitats and different collecting periods].to determine the contents of hesperidin, nobiletin and tangeretin in citrus reticulata 'chachi' from various habitats and different collecting periods (from october to december) and study the dynamic change of three flavonoids constituents.201020575406
[determination of the eliminating ratio of tcm polysaccharide to hydroxyl radical by kinetic fluorescent spectrophotometry].to explore reaction dynamic of hydroxyl radical (*oh) and salicylic acid,and to determine the elimination ratios of tcm polysaccharide to *oh by kinetic fluorescent analysis.200919873737
carotenoid content impacts flavor acceptability in tomato (solanum lycopersicum).tomatoes contain high levels of several carotenoids including lycopene and β-carotene. beyond their functions as colorants and nutrients, carotenoids are precursors for important volatile flavor compounds. in order to assess the importance of apocarotenoid volatiles in flavor perception and acceptability, we conducted sensory evaluations of near-isogenic carotenoid biosynthetic mutants and their parent, ailsa craig.201020661902
euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber holothuria edulis, and proposal of euzebyaceae fam. nov., euzebyales ord. nov. and nitriliruptoridae subclassis nov.a tangerine-coloured, gram-positive actinobacterial strain, designated f10(t), was isolated from the abdominal epidermis of a sea cucumber, holothuria edulis, collected in seawater off the coast of japan. a 16s rrna gene sequence analysis indicated that strain f10(t) was a member of the class actinobacteria and was most closely related to nitriliruptor alkaliphilus anl-iso2(t) (87.4 % sequence similarity). phylogenetic analyses showed that strain f10(t) represented a novel, deep-rooted, and dist ...201019915105
simultaneous determination of four 5-hydroxy polymethoxyflavones by reversed-phase high performance liquid chromatography with electrochemical detection.accumulating evidence has suggested the potential health-promoting effects of 5-hydroxy polymethoxyflavones (5-oh-pmfs) naturally existing in citrus genus. however, research efforts are hampered by the lack of reliable and sensitive methods for their determination in plant materials and biological samples. using reversed-phase high performance liquid chromatography (hplc) equipped with electrochemical (ec) detection, we have developed a fast and highly sensitive method for quantification of four ...201020022018
the fus3 mapk signaling pathway of the citrus pathogen alternaria alternata functions independently or cooperatively with the fungal redox-responsive ap1 regulator for diverse developmental, physiological and pathogenic processes.alternaria alternata, the fungus that causes citrus brown spot, invades its hosts primarily through the production and action of a host-selective act toxin that kills citrus cells prior to invasion. in this study, we show that, in the tangerine pathotype of a. alternata, a mitogen-activated protein kinase (mapk)-mediated signaling pathway governs a number of biological functions, either separately or in a cooperative manner, with the aaap1 gene encoding a transcription regulator. the reported ma ...201020036749
effect of ploidy increase on transgene expression: example from citrus diploid cybrid and allotetraploid somatic hybrid expressing the egfp gene.polyploidization is an important speciation mechanism for all eukaryotes, and it has profound impacts on biodiversity dynamics and ecosystem functioning. green fluorescent protein (gfp) has been used as an effective marker to visually screen somatic hybrids at an early stage in protoplast fusion. we have previously reported that the intensity of gfp fluorescence of regenerated embryoids was also an early indicator of ploidy level. however, little is known concerning the effects of ploidy increas ...201020734092
temporal patterns in homalodisca spp. (hemiptera: cicadellidae) oviposition on southern california citrus and jojoba.a detailed study of the distribution of egg masses of homalodisca vitripennis (germar) and h. liturata ball was done across a 2-yr period (2001-2003) on six host plants in southern california (marsh grapefruit, lisbon lemon, washington navel, dancy tangerine, rough lemon, and jojoba in riverside; jojoba in desert center). the majority of egg masses in riverside belonged to h. vitripennis (84-100%), whereas in desert center, all homalodisca egg masses were h. liturata. oviposition in riverside oc ...201020146836
actts3 encoding a polyketide synthase is essential for the biosynthesis of act-toxin and pathogenicity in the tangerine pathotype of alternaria alternata.the tangerine pathotype of alternaria alternata produces host-selective act-toxin and causes alternaria brown spot disease of tangerine and tangerine hybrids. sequence analysis of a genomic bac clone identified part of the act-toxin tox (actt) gene cluster, and knockout experiments have implicated several open reading frames (orf) contained within the cluster in the biosynthesis of act-toxin. one of the orf, designated actts3, encoding a putative polyketide synthase, was isolated by rapid amplif ...201020192828
aroma character impact compounds in kinokuni mandarin orange (citrus kinokuni) compared with satsuma mandarin orange (citrus unshiu).the odor-active volatiles of kinokuni mandarin (citrus kinokuni hort. ex tanaka), an original mandarin orange in japan, were characterized by a combination of instrumental and sensory analyses and compared with those of satsuma mandarin (citrus unshiu marcovitch). an aroma extract dilution analysis (aeda) of the polar fractions of kinokuni and satsuma mandarin peel oils identified five odorants in common as the most odor-active volatiles: (z)-hex-3-enal, decanal, linalool, yuzuol, and (2e)-trans ...201020378969
effect of citrus reticulata and cymbopogon citratus essential oils on aspergillus flavus growth and aflatoxin production on asparagus racemosus.essential oils extracted from citrus reticulata and cymbopogon citratus were tested in vitro against the toxigenic strain of aspergillus flavus, isolated from the tuberous roots of asparagus racemosus, used in preparation of herbal drugs. the essential oils completely inhibited the growth of a. flavus at 750 ppm and also exhibited a broad fungitoxic spectrum against nine additional fungi isolated from the roots. citrus reticulata and cymbopogon citratus essential oils completely inhibited aflato ...201020401550
polymethoxylated flavones, flavanone glycosides, carotenoids, and antioxidants in different cultivation types of tangerines ( citrus reticulata blanco cv. sainampueng) from northern thailand.polymethoxylated flavones (pmfs) and flavanone glycosides (fgs) were analyzed in hand-pressed juice and the peeled fruit of 'sainampueng' tangerines ( citrus reticulata blanco cv. sainampueng) grown in northern thailand. the tangerines were collected from a citrus cluster of small orchard farmers and were cultivated as either agrochemical-based (ab), agrochemical-safe (as), or organic grown fruits. juice samples were also measured on contents of carotenoids, ascorbic acid, and tocopherols. the p ...201020420369
spray deposition and efficacy of four petroleum-derived oils used against tetranychus urticae (acari: tetranychidae).petroleum-derived spray oils (pdsos) offer an interesting alternative to acaricides to control the twospotted spider mite, tetranychus urticae koch (acari: tetranychidae), a key pest of clementine mandarins, citrus reticulata blanco. however, there is a lack of knowledge on how these products should be used. in this study, we evaluated the efficacy of four pdsos (sunspray ultrafine, volck miscible, texaco d-c-tron plus, and agroaceite) at five concentrations (0.5, 1.0, 1.5, 2.0, and 3.0%) agains ...201020429453
spermine pretreatment confers dehydration tolerance of citrus in vitro plants via modulation of antioxidative capacity and stomatal response.polyamines, small aliphatic polycations, have been suggested to play key roles in a number of biological processes. in this paper, attempts were made to investigate the possibility of improving dehydration tolerance of citrus in vitro plants by exogenous application of spermine (spm). 'red tangerine' (citrus reticulata blanco) in vitro plants pretreated with 1 mm spm exhibited less wilted phenotype and lower water loss and electrolyte leakage than the control under dehydration. spm-pretreated pl ...201020462936
carotenoid profiles in provitamin a-containing fruits and vegetables affect the bioefficacy in mongolian gerbils.fruits and vegetables are rich sources of provitamin a carotenoids. we evaluated the vitamin a (va) bioefficacy of a whole foods supplement (wfs) and its constituent green vegetables (study 1) and a variety of fruits with varying ratios of provitamin a carotenoids (study 2) in va-depleted mongolian gerbils (n = 77/study). after feeding a va-deficient diet for 4 and 6 weeks in studies 1 and 2, respectively, customized diets, equalized for va, were fed for 4 and 3 weeks, respectively. both studies ...201020558838
rapid simultaneous determination of amines and organic acids in citrus using high-performance liquid chromatography.rapid analytical method for the simultaneous separation and determination of amines and organic acids is a vital interest for quality control of citrus and their products. in the present study, a simultaneous high performance liquid chromatography (hplc) method for the rapid separation of three amines and two organic acids was developed. chromatographic separation of compounds was achieved using xbridge c(18) column at ambient temperature, with an isocratic mobile phase of 3mm phosphoric acid at ...201021147342
molecular systematics of citrus-associated alternaria species.the causal agents of alternaria brown spot of tangerines and tangerine hybrids, alternaria leaf spot of rough lemon and alternaria black rot of citrus historically have been referred to as alternaria citri or a. alternata. ten species of alternaria recently were described among a set of isolates from leaf lesions on rough lemon (citrus jambhiri) and tangelo (c. paradisi × c. reticulata), and none of these isolates was considered representative of a. alternata or a. citri. to test the hypothesis ...200421148834
phylogenetic and evolutionary analysis of nbs-encoding genes in rutaceae fruit crops.the nucleotide-binding site leucine-rich repeat (nbs-lrr) genes are the largest class of disease resistance genes in plants. however, our understanding of the evolution of nbs-lrr genes in rutaceae fruit crops is rather limited. we report an evolutionary study of 103 nbs-encoding genes isolated from poncirus trifoliata (trifoliate orange), citrus reticulata (tangerine) and their f(1) progeny. in all, 58 of the sequences contained a continuous open reading frame. phylogenetic analysis classified ...201021153735
bacillus luteolus sp. nov., a halotolerant bacterium isolated from a salt field.a novel gram-stain-positive, motile, strictly aerobic bacterium, designated yim 93174(t), was isolated from a salt field in korea. cells of this strain were rod-shaped and formed pale tangerine colonies and grew at ph 6.0-8.0 (optimal growth at ph 7.0), at 15-45 °c (optimum 28-37 °c) and at salinities of 0-10 % (w/v) nacl (optimum 0-2 % nacl). some phenotypic characters allowed differentiation of strain yim 93174(t) from its nearest phylogenetic relatives. comparative 16s rrna gene sequence anal ...201020584813
chemical composition and larvicidal activity of edible plant-derived essential oils against the pyrethroid-susceptible and -resistant strains of aedes aegypti (diptera: culicidae).the chemical compositions and larvicidal potential against mosquito vectors of selected essential oils obtained from five edible plants were investigated in this study. using a gc/ms, 24, 17, 20, 21, and 12 compounds were determined from essential oils of citrus hystrix, citrus reticulata, zingiber zerumbet, kaempferia galanga, and syzygium aromaticum, respectively. the principal constituents found in peel oil of c. hystrix were beta-pinene (22.54%) and d-limonene (22.03%), followed by terpinene ...201020618656
distribution of aroma volatile compounds in tangerine hybrids and proposed inheritance.with the desirable combination of sugars and acids, volatile compounds contribute to the essential organoleptic attributes of citrus. this study evaluated the aroma volatiles of 20 tangerine hybrids of the university of florida breeding program. volatiles were sampled from hand-squeezed juice by headspace solid-phase microextraction (spme), and analyzed by gas chromatography-mass spectrometry. principal component analysis (pca) and cluster analysis (ca) were used to find similarities among sampl ...201021218478
somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the mediterranean citrus industry.the prevalence of sour orange rootstock in the southern and eastern part of the mediterranean basin is presently threatened by the spread of citrus tristeza virus (ctv) and its main vector toxoptera citricida, combined with abiotic constraints such as drought, salinity and alkalinity. the search for alternative ctv-resistant rootstocks that also withstand the other constraints is now considered an urgent priority for a sustainable citrus industry in the area. complementary progenitors can be fou ...201121225429
simultaneous determination of flavonoids in different parts of citrus reticulata 'chachi' fruit by high performance liquid chromatography-photodiode array detection.flavonoids are important polyphenolic secondary metabolites in plant. citrus reticulata 'chachi' fruit are rich in flavonoids and are being used as functional antioxidant ingredients for the treatment of atherosclerosis and cancer, etc. a high performance liquid chromatography-photodiode array detection system was used to analyze five flavonoids, namely, naringin, hesperidin, didymin, tangeretin and nobiletin, in different parts of c. reticulata 'chachi' fruit. the chromatographic analysis was p ...201020714303
cloning and expression analysis of s-rnase homologous gene in citrus reticulata blanco cv. wuzishatangju.s-rnase-based self-incompatibility is the most widespread form of genetically controlled mate selection in plants and that s-rnase controls pollination specificity in the pistils. 'wuzishatangju' (citrus reticulata blanco), a nature bud mutant from a self-compatible (sc) cultivar 'shatangju', displays gametophytic self-incompatibility (gsi). in this study, full-length sequences of cdna and dna of the s-rnase homologous gene were obtained from 'wuzishatangju' and 'shatangju'. there was no differe ...201121421381
juice components and antioxidant capacity of four tunisian citrus varieties.juices from four citrus species of tunisia were investigated mainly for quality parameters and antioxidant capacity.201120862741
enhanced ethanol production from kinnow mandarin (citrus reticulata) waste via a statistically optimized simultaneous saccharification and fermentation process.dried, ground, and hydrothermally pretreated kinnow mandarin (citrus reticulata) waste was used to produce ethanol via simultaneous saccharification and fermentation (ssf). central composite design was used to optimize cellulase and pectinase concentrations, temperature, and time for ssf. the d-limonene concentration determined with high-performance liquid chromatography (hplc) for fresh, dried, and pretreated biomass was 0.76%, 0.32%, and 0.09% (v/w), respectively. design expert software sugges ...201020863699
citrus fruit extracts reduce advanced glycation end products (ages)- and h(2)o(2)-induced oxidative stress in human adipocytes.diabetes is a reactive oxygen species (ros)-mediated pathology, with a worldwide prevalence estimated to double by 2030. a major effort has been launched to find therapeutic means to improve health conditions of diabetic patients. recent data show that supplemental natural antioxidants represent a potential strategy as adjunct therapy. despite the major role of adipocytes in the etiology of diabetes, little is known about the effect of natural antioxidants on adipocyte response to oxidative stre ...201020882960
do epidermal lens cells facilitate the absorptance of diffuse light?many understory plants rely on diffuse light for photosynthesis because direct light is usually scattered by upper canopy layers before it strikes the forest floor. there is a considerable gap in the literature concerning the interaction of direct and diffuse light with leaves. some understory plants have well-developed lens-shaped epidermal cells, which have long been thought to increase the absorption of diffuse light. to assess the role of epidermal cell shape in capturing direct vs. diffuse ...200721636475
effective sampling range of a synthetic protein-based attractant for ceratitis capitata (diptera: tephritidae).studies were conducted in honduras to determine effective sampling range of a female-targeted protein-based synthetic attractant for the mediterranean fruit fly, ceratitis capitata (wiedemann) (diptera: tephritidae). multilure traps were baited with ammonium acetate, putrescine, and trimethylamine lures (three-component attractant) and sampled over eight consecutive weeks. field design consisted of 38 traps (over 0.5 ha) placed in a combination of standard and high-density grids to facilitate ge ...201021061993
a new limonoid from the seeds of citrus reticulata blanco.a new limonoid, named citriolide-a (1), along with the known structurally related compounds 2-5, was isolated from the liposoluble extract of the seeds of citrus reticulata blanco. the structure of citriolide-a (1) was elucidated by means of ei, 1d- and 2d-nmr techniques. compounds 1, 2 and 4 exhibited medium cytotoxicity against p-388 and a-549 cell lines.201121834637
use of a multivariate approach to assess the incidence of alicyclobacillus spp. in concentrate fruit juices marketed in argentina: results of a 14-year survey.the purpose of this study was to determine the incidence of alicyclobacillus spp. in fruit/vegetable juices (concentrated pulps and clarified and non-clarified juices) marketed in argentina between 1996 and 2009. the presence of alicyclobacillus was determined in a total of 8556 samples of fruit and vegetable juices (apple, pear, grape, peach, blend of juices, tangerine, pineapple, orange, mango, plum, guava, apricot, lemon, banana, kiwi, carrot, strawberry, grapefruit, and beetroot) collected i ...201121962940
[consumption to feed of resident adult population in rural area of the city of ibatiba (es, brazil)].it is a transverse study where a questionnaire of alimentary frequency was applied (qaf) in 150 adults resident of the rural area of the city of ibatiba (es, brazil). qaf classified the alimentary consumption as: habitual (>4 times in the week), not habitual (<4 times in the week) and rarely (1 time a month), with objective of correlating the alimentary consumption with the chronic-degenerative diseases. the results evidenced a habitual consumption of rice, breads, stalk, bean, cow milk, animal ...201121180826
[Study of xiaozhang recipe combined with lamivudine in treatment of 84 chronic viral hepatitis B patients with compensated liver cirrhosis].To assess the clinical efficacy of Xiaozhang Recipe (XR) in treatment of chronic viral hepatitis B patients with compensated liver cirrhosis.201122013800
citrus limonin and its glucoside inhibit colon adenocarcinoma cell proliferation through apoptosis.the current study was an attempt to elucidate the mechanism of human colon cancer cell proliferation inhibition by limonin and limonin glucoside (lg) isolated from seeds of citrus reticulata. the structures of purified compounds were confirmed by nmr and quantified using hplc. these compounds of more than 95% purity were subjected to proliferation inhibition assay using human colon adenocarcinoma (sw480) cells. the ic50 value of 54.74 and 37.39 µm was observed for limonin and lg, respectively at ...201121338095
characterization and comparison of volatile constituents of juice and peel from clementine, mandarin and their hybrids.the volatile compositions of juice and peel of clementine (citrus reticulata x citrus sinensis var. commune), mandarin (citrus reticulata blanco var. willow leaf) and their hybrids were analyzed by headspace solid-phase extraction (hs-spme) coupled with gas chromatography and either a flame ionization detector (fid) or a mass spectrometer (ms). the major compounds of the volatile fractions of clementine and mandarin were limonene and limonene/gamma-terpinene, respectively. the volatile compositi ...201122164792
microarray expression profiling of postharvest ponkan mandarin (citrus reticulata) fruit under cold storage reveals regulatory gene candidates and implications on soluble sugars metabolism.low temperature storage is widely applied to maintain citrus postharvest fruit quality. in this study, the transcriptional and metabolic changes in the pulp tissue of citrus reticulata blanco cv. "ponkan" were studied for three successive months under cold storage by affymetrix citrus genechip and gas chromatography, respectively. as many as 2 161 differentially expressed transcripts were identified based on the bayesian hierarchical model. the statistical analysis of gene ontology revealed that ...201121348940
beta-cryptoxanthin, plentiful in japanese mandarin orange, prevents age-related cognitive dysfunction and oxidative damage in senescence-accelerated mouse brain.increased oxidative stress is known to accelerate age-related pathologies. beta-cryptoxanthin (ß-crx, (3r)-ß,ß-caroten-3-ol) is a potent antioxidant that is highly rich in satsuma mandarin orange (mandarin), which is the most popular fruit in japan. we investigated the antioxidative and anti-aging effects of ß-crx and mandarin using senescence-accelerated mice (samp10), which were characterized by a short lifespan, high generation of superoxide anions in the brain and poor learning ability with ...201121372377
Separation and purification of neohesperidin from the albedo of Citrus reticulata cv. Suavissima by combination of macroporous resin and high-speed counter-current chromatography.In this article, a simple and efficient protocol for rapid preparation and separation of neohesperidin from the albedo of Citrus reticulata cv. Suavissima was established by the combination of macroporous resin column chromatography and high-speed counter-current chromatography (HSCCC). Six types of resin were investigated by adsorption and desorption tests, and D101 macroporous resin was selected for the first cleaning-up procedure, in which 55% aqueous ethanol was used to elute neohesperidin. ...201122102373
comparative study on antioxidant activity of lycopene (z)-isomers in different assays.several studies have implicated the potent antioxidant properties of lycopene. however, most of the studies used only the (all-e)-isomer. (z)-isomers of lycopene were found in substantial amounts in processed foods and in human tissues. in the present study, we investigated in vitro the antioxidant activity of (5z)-, (9z)-, and (13z)-lycopene compared to the (all-e)-isomer. additionally, prolycopene, the (7z,9z,7'z,9'z)-isomer found in tangerine tomatoes, was analyzed. no significant differences ...201121476575
inhibition of tnf-α/ifn-γ induced rantes expression in hacat cell by naringin.context: naringin is a bioflavonoid derivative and is predominantly found in citrus paradisi macf., citrus sinensis (linn.) osbeck, citrus unshiu marc., citrus reticulata blanco cv. nobilis, citrus tachibana (makino) tanaka, citrus junos sieb. ex tanaka (rutaceae), and related citrus species. it has anti-inflammatory effects that have been well-documented, but the mechanism is poorly characterized. objective: the effect of naringin on production of rantes (regulated upon activation normal t-cell ...201121500970
callus cultures of tomato mutants: i. nutritional requirements.callus from hypocotyl, stem, and fruit tissue of tomato mutants was grown on a complex pea extract medium. the genotypes responded differently to the levels of nutrients and stimulators or inhibitors in the medium. hypocotyl callus of yellow (r) tomato required k(2) so(4) for quick establishment and continued steady growth for several months; callus of this mutant could also grow with 0.5 % dimethyl sulfoxide in the medium, although growth was less than the control. the red ghost (r(+) gh) mutan ...196920925678
characterization of microsatellite markers in mandarin orange (citrus reticulata blanco).a dinucleotide-enriched genomic library was obtained from mandarin orange (citrus reticulata blanco). a subset of 101 positive clones was sequenced and primers were designed. the loci were screened for levels of variation using 26-29 wild mandarin oranges collected in vietnam. forty-three loci were polymorphic with the number of alleles ranging from two to 18. the observed heterozygosity (h(o) ) and expected heterozygosity (h(e) ) were from 0.03 to 0.96 and from 0.03 to 0.92, respectively.200821585732
cellular responses required for oxidative stress tolerance, colonization, and lesion formation by the necrotrophic fungus alternaria alternata in citrus.the pathogenic capability of the tangerine pathotype of alternaria alternata relies on the production of host-selective act toxin. inoculation of a. alternata in leaves of the citrus quickly induced rapid lipid peroxidation, accumulation of hydrogen peroxide (h(2)o(2)), and cell death, indicative of host defensive response. we previously demonstrated an essential role of the a. alternata aaap1 gene, encoding a redox-responsive yap1-like transcription factor, to contribute to fungal pathogenicity ...201020978890
[Influences of erectile functions in benign prostatic hyperplasia patients by two micro (thulium) laser resection of prostate-tangerine technique].To observe the influences of erectile dysfunction (ED) by age, prostate size and lower urinary tract symptom (LUTS) undergoing two micro (thulium) laser resection of prostate-tangerine technique (TmLRP-TT).201122094087
complete genome sequence of mandarin decline citrus tristeza virus of the northeastern himalayan hill region of india: comparative analyses determine recombinant.the complete genome sequence of a mandarin (citrus reticulata) decline ctv isolate, kpg3, of the darjeeling hills of the northeastern himalayan region of india is reported for the first time. the complete kpg3 genome has 19253 nt, and its nucleotide sequence identity ranged from 79% with the florida ctv isolate t36 to 94% with the israel isolate vt, whereas its identity to b165, the other indian isolate, was 89%. phylogenetic analysis indicated that the kpg3 genome is closely related to isolate ...201122160128
mechanism of visceral fat reduction in tsumura suzuki obese, diabetes (tsod) mice orally administered β-cryptoxanthin from satsuma mandarin oranges ( citrus unshiu marc).the carotenoid β-cryptoxanthin (β-crx) is abundant in satsuma mandarins ( citrus unshiu marc). several studies have shown a relationship between satsuma mandarin consumption and a low risk of several diseases, for example, diabetes, gout, and hypertension, suggesting β-crx involvement in disease prevention. we investigated the effect of β-crx on mildly obese males. β-crx administration reduced visceral adipose tissue, body weight, and abdominal circumference. however, the detailed mechanism by ...201122085304
subacute toxicity assessment of carotenoids extracted from citrus peel (nanfengmiju, citrus reticulata blanco) in rats.the mixture of carotenoids extracted from citrus peel (nanfengmiju, citrus reticulata blanco) was tested for subacute oral toxicity. in this study, dose levels of 0, 200, 500 and 2000mg/kg body weight/day were administered by gavage to 10 wistar rats/sex/group for 28days. no statistically significant, dose-related effect on food consumption, food efficiency, body weight gain, clinical signs or ophthalmoscopic parameters was observed in any treatment group. urinalysis, hematological, blood coagul ...201122197624
effect of the nematode biocide dbx-1003 in controlling citrus nematode infecting mandarin, and interrelationship with the co-inhabitant fungi.in a field experiment, the nematode-biocide, dbx-1003, was evaluated against the citrus nematode, tylenchulus semipenetrans infecting mandarin, citrus reticulata, dbx-1003 20% g., was applied in october 2008 at the rate 1/2 kg/tree, and root and soil samples were collected monthly until the next october, 2009. successive treatment of the same biocide was added in may 2009. for the comparison, vydate 24% l, was applied as well as non-treated check trees were left. data revealed that the biocide t ...201325151818
cold treatment of ceratitis capitata (diptera: tephritidae) in oranges using a larval endpoint.south africa currently exports fresh citrus (citrus spp.) fruit to japan using an in-transit cold treatment protocol of 14 d or 12 d at temperatures <0 degrees c for treatment of ceratitis capitata (wiedemann) (diptera: tephritidae) in 'clementine' mandarins (citrus reticulata blanco) and other citrus types, respectively. to reduce the risk of chilling injury with this treatment, research was conducted with temperatures >0 degrees c. earlier south african research had shown that young (6-d-old) ...201121882680
exploiting bac-end sequences for the mining, characterization and utility of new short sequences repeat (ssr) markers in citrus.the aim of this study was to develop a large set of microsatellite markers based on publicly available bac-end sequences (bess), and to evaluate their transferability, discriminating capacity of genotypes and mapping ability in citrus. a set of 1,281 simple sequence repeat (ssr) markers were developed from the 46,339 citrus clementina bac-end sequences (bes), of them 20.67% contained ssr longer than 20 bp, corresponding to roughly one perfect ssr per 2.04 kb. the most abundant motifs were di-nuc ...201122170603
susceptibilities of candida albicans mouth isolates to antifungal agents, essentials oils and mouth rinses.forty candida albicans strains isolated from patient's mouth with fixed orthodontic appliances were analyzed to their susceptibilities to antifungal agents, mouth rinses and essential oils. susceptibility to fluconazole, econazole, miconazole and ketoconazole, amphotericin b and nystatin was assessed by the disk diffusion (dd) method based on the clinical and laboratory standards institute m44-a protocol, and by etest (fluconazole and amphotericin b). the susceptibilities to mouth rinses and ess ...201222246961
the slt2 mitogen-activated protein kinase-mediated signalling pathway governs conidiation, morphogenesis, fungal virulence and production of toxin and melanin in the tangerine pathotype of alternaria alternata.fungi respond and adapt to different environmental stimuli via signal transduction systems. we determined the function of a yeast slt2 mitogen-activated protein (map) kinase homologue (aaslt2) in alternaria alternata, the fungal pathogen of citrus. analysis of the loss-of-function mutant indicated that aaslt2 is required for the production of a host-selective toxin, and is crucial for fungal pathogenicity. moreover, the a. alternata slt2 mutants displayed hypersensitivity to cell wall-degrading ...201121726368
comparison of cell wall metabolism in the pulp of three cultivars of 'nanfeng' tangerine differing in mastication trait.background: like sweet orange (citrus sinensis), tangerine (citrus reticulata) is another citrus crop grown widely throughout the world. however, whether it shares a common mechanism with sweet orange in forming a given mastication trait is still unclear. in this study, three 'nanfeng' tangerine cultivars, 'yangxiao-26' ('yx-26') with inferior mastication trait, elite 'yx-26' with moderate mastication trait and 'miguang' ('mg') with superior mastication trait, were selected to investigate the fo ...201121732384
effect of larval density and host species on preimaginal development of bactrocera tau (diptera: tephritidae).the fruit fly, bactrocera tau (walker) (diptera: tephritidae), is an important pest of fruit and vegetable crops. in this study, host preference of b. tau females and the effects of host species and larval density on larval survival, pupal weight, adult emergence, and developmental duration were investigated on cucumber (cucumis sativus l.), sponge gourd (luffa cylindrical l. (roem)), bitter gourd [momordica charantia (cucurbitaceae) l.], guava [psidium guajava (myrtaceae) l.], and tangerine [ci ...201122299343
effect of juice extraction methods and processing temperature-time on juice quality of nagpur mandarin (citrus reticulata blanco) during storage.influence of juice extraction methods and pasteurization temperature and time on quality of mandarin (citrus reticulata blanco) juice was studied. the experiment consisted of 65 °c pasteurization temperature with 15, 25 and 35 min holding time; 75 °c with 10, 20 and 30 min and 85 °c with 5, 10 and 15 min holding times and two types of juice extraction methods. the experiment was laid out in factorial completely randomized design with three replications. juice extracted with screw type juice extr ...201123572734
physico-chemical, respiratory and fungicide residue changes in wax coated mandarin fruit stored at chilling temperature with intermittent warming.influence of chilling temperature, intermittent warming (iw) and fungicidal wax coating was evaluated during storage of 'nagpur' mandarins (citrus reticulata blanco). fruits were light green coloured with slight colour-break at the start of storage. waxed and non-waxed fruits were stored at 3.5 °c (constant), 2 weeks at 3.5 °c followed by iw for 1 week at 19.5 °c (cycle) and at 6.5 °c (constant), and were evaluated immediately after 30, 45, 60, 75 days and also after 1 week holding at ambient co ...201123572729
genome sequence of streptomyces wadayamensis strain a23, an endophytic actinobacterium from citrus reticulata.the actinobacterium streptomyces wadayamensis a23 is an endophyte of citrus reticulata that produces the antimycin and mannopeptimycin antibiotics, among others. the strain has the capability to inhibit xylella fastidiosa growth. the draft genome of s. wadayamensis a23 has ~7.0 mb and 6,006 protein-coding sequences, with a 73.5% g+c content.201424994795
house fly (musca domestica l.) attraction to insect honeydew.house flies are of major concern as vectors of food-borne pathogens to food crops. house flies are common pests on cattle feedlots and dairies, where they develop in and feed on animal waste. by contacting animal waste, house flies can acquire human pathogenic bacteria such as escherichia coli and salmonella spp., in addition to other bacteria, viruses, or parasites that may infect humans and animals. the subsequent dispersal of house flies from animal facilities to nearby agricultural fields co ...201525970333
biological control of toxigenic citrus and papaya-rotting fungi by streptomyces violascens mt7 and its extracellular metabolites.an indian indigenous, loktak lake soil isolate streptomyces violascens mt7 was assessed for its biocontrol potential both in vitro and in vivo against toxigenic fruit-rotting fungi. strain mt7 exhibited broad-spectrum antifungal activity against various pathogenic postharvest fungi of citrus and papaya. in shake-flask fermentation, antagonist s. violascens mt7 highly produced extracellular antifungal metabolites in early stationary growth phase in glucose-yeast extract-malt extract (m93) broth. ...201526214840
structure-activity relationship of citrus polymethoxylated flavones and their inhibitory effects on aspergillus niger.citrus peels are rich in polymethoxylated flavones (pmfs) and are potential sources of natural preservatives. six pmfs extracts, isolated and purified from the peels of three mandarins (citrus reticulata) and three sweet oranges (citrus sinensis), were identified and quantitated. their inhibitory effects on aspergillus niger were evaluated using a microbroth dilution assay. the red tangerine variety exhibited the greatest antifungal activity (mic = 0.2 mg/ml), while jincheng showed the lowest ac ...201222500738
isolation and characterization of putative endophytic bacteria antagonistic to phoma tracheiphila and verticillium albo-atrum.a collection of 200 bacterial isolates recovered from citrus plants (citrus limon, citrus sinensis, and citrus reticulata), medicago truncatula and laurus nobilis, was established. in vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens phoma tracheiphila and verticillium albo-atrum. isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (pgpb) abilities, as well as for the presence of nonribosomal pe ...201425074353
spatial and temporal spread of citrus tristeza virus and its aphid vectors in the north western area of morocco.first report of citrus tristeza virus (ctv,closterovirus) in morocco datesback to 1961 in collections of citrus varieties. an exhaustive survey of citrus in the north of the country in 2009 revealed that ctv was spread all over the citrus production area. we attempted to evaluate the relative contribution of different aphid species in the spread of ctv disease in a citrus reticulata orchard at the loukkous region during 2 years (2012 and 2013). the overall ctv incidence estimated in the experime ...201625884375
isolation, screening, characterization, and selection of superior rhizobacterial strains as bioinoculants for seedling emergence and growth promotion of mandarin orange (citrus reticulata blanco).mandarin orange (mo) is an important fruit crop of tropical and subtropical regions of the world. a total of 217 morphologically distinct rhizobacteria from mo orchards in 3 states of northeastern india were isolated and analyzed for 4 plant-growth-promoting (pgp) attributes: nitrogen fixation, production of indole acetic acid like substances, solubilization of phosphate, and ability to antagonize pathogenic fungi. isolates were ranked based on in-vitro-assayed pgp attributes, and 10 superior is ...201424498985
polyphenolic content and bactericidal effect of mexican citrus limetta and citrus reticulata.in this study, total phenolics, total flavonoids, hesperidin and ascorbic acid contents in bagasse, juice and seed of mexican sweet lime (citrus limetta) and mandarine (citrus reticulata) were determined at two commercial maturity stages (maturation index), as well as their bactericidal effect on escherichia coli and staphylococcus aureus. the results showed that bagasses had the highest total phenolics, total flavonoids, and hesperidin content for both, c. limetta, and c. reticulata; highest as ...201728242952
potential risks resulting from fruit/vegetable-drug interactions: effects on drug-metabolizing enzymes and drug transporters.it has been well established that complex mixtures of phytochemicals in fruits and vegetables can be beneficial for human health. moreover, it is becoming increasingly apparent that phytochemicals can influence the pharmacological activity of drugs by modifying their absorption characteristics through interactions with drug transporters as well as drug-metabolizing enzyme systems. such effects are more likely to occur in the intestine and liver, where high concentrations of phytochemicals may oc ...201122417366
transcriptional response of susceptible and tolerant citrus to infection with candidatus liberibacter asiaticus.candidatus liberibacter asiaticus (las), a non-culturable phloem-limited bacterium, is the suspected causal agent of huanglongbing (hlb) in florida. hlb is one of the most devastating diseases of citrus and no resistant cultivars have been identified to date, though tolerance has been observed in the genus poncirus and some of its hybrids. this study compares transcriptional changes in tolerant us-897 (citrus reticulata blanco×poncirus trifoliata l. raf.) and susceptible 'cleopatra' mandarin (c. ...201122325873
comparison of antifungal activities of vietnamese citrus essential oils.citrus essential oils (eos) are volatile compounds from citrus peels and widely used in perfumes, cosmetics, soaps and aromatherapy. in this study, inhibition of citrus eos extracted from vietnamese orange (citrus sinensis), mandarin (citrus reticulata blanco), pomelo (citrus grandis osbeck) and lime (citrus aurantifolia swingle) on the growth of plant pathogenic fungi, mucor hiemalis, penicillium expansum and fusarium proliferatum was investigated. the eos of the citrus peels were obtained by c ...201322799453
evaluation of skin anti-aging potential of citrus reticulata blanco peel.the peel of citrus reticulata blanco is traditionally used as tonic, stomachic, astringent, and carminative. it is also useful in skin care.201727365982
synergistic combinations of high hydrostatic pressure and essential oils or their constituents and their use in preservation of fruit juices.this work addresses the inactivation achieved with escherichia coli o157:h7 and listeria monocytogenes egd-e by combined processes of high hydrostatic pressure (hhp) and essential oils (eos) or their chemical constituents (ccs). hhp treatments (175-400 mpa for 20 min) were combined with 200 μl/l of each eo (citrus sinensis l., citrus lemon l., citrus reticulata l., thymus algeriensis l., eucalyptus globulus l., rosmarinus officinalis l., mentha pulegium l., juniperus phoenicea l., and cyperus lo ...201323246609
characterization of pseudomonas syringae pv. syringae, causal agent of citrus blast of mandarin in montenegro.citrus blast caused by bacterium pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. considering the above fact, this study reports genetic characterization of 43 p. syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (citrus reticulata) in montenegro, using multilocus sequence analysis of gyrb, rpod, and gap1 gene sequences. gene sequences from a ...201728167885
storage stability of kinnow fruit (citrus reticulata) as affected by cmc and guar gum-based silver nanoparticle coatings.the influence of carboxy methyl cellulose (cmc) and guargum-based coatings containing silver nanoparticles was studied on the postharvest storage stability of the kinnow mandarin (citrus reticulata cv. blanco) for a period of 120 days (85%-90% relative humidity) at 4 °c and 10 °c. physicochemical and microbiological qualities were monitored after every 15 days of storage. overall results revealed an increase in total soluble solid (tss), total sugars, reducing sugars and weight loss but this inc ...201526694344
botanicals, selective insecticides, and predators to control diaphorina citri (hemiptera: liviidae) in citrus orchards.the asian citrus psyllid (acp) diaphorina citri kuwayama vectors pathogens that cause huanglongbing (hlb) or citrus greening devastating and economically important disease present in most citrus growing regions. young citrus shoots are required for psyllid reproduction and development. during winter citrus trees produce little or no new growth. overwintering adults reproduce in spring on newly emerging shoots also attractive to other pests and beneficial insects. botanicals and relatively select ...201425205398
transcript profiling analysis of rhodosporidium paludigenum-mediated signalling pathways and defense responses in mandarin orange.to investigate the basis of inducible resistance response in postharvest mandarin orange, cdna microarray and high-performance liquid chromatography were performed to study transcriptional and metabolic changes in rhodosporidium paludigenum strain treated fruit. the microarray data mining revealed that r. paludigenum activated transcription of genes important for plant hormones, signalling transduction, stress and defensive responses in orange peel tissue. moreover, up-regulation of phenylalanin ...201525442597
schistosomicidal effects of the essential oils of citrus limonia and citrus reticulata against schistosoma mansoni.we report the in vitro schistosomicidal effects of the essential oil obtained from citrus limonia leaves (cl-eo) and c. reticulata fruit peels (cr-eo), cultivated in brazil, against schistosoma mansoni worms. limonene (29.9%), β-pinene (12.0%), sabinene (9.0%), citronellal (9.0%), and citronellol (5.8%) are the major constituents of cl-eo; limonene (26.5%), γ-terpinene (17.2%), linalool (11.1%), octanal (8.0%), myrcene (6.2%), and capraldehyde (3.9%) predominate in cr-eo. cl-eo displayed moderat ...201727936310
rna-seq analysis of citrus reticulata in the early stages of xylella fastidiosa infection reveals auxin-related genes as a defense response.citrus variegated chlorosis (cvc), caused by xylella fastidiosa, is one the most important citrus diseases, and affects all varieties of sweet orange (citrus sinensis l. osb). on the other hand, among the citrus genus there are different sources of resistance against x. fastidiosa. for these species identifying these defense genes could be an important step towards obtaining sweet orange resistant varieties through breeding or genetic engineering. to assess these genes we made use of mandarin (c ...201324090429
characterization of three linalool synthase genes from citrus unshiu marc. and analysis of linalool-mediated resistance against xanthomonas citri subsp. citri and penicilium italicum in citrus leaves and fruits.three cdna clones from satsuma mandarin (citrus unshiu marc.) were isolated and expressed in escherichia coli. custs3-1 and custs3-2 encode linalool synthases and custs4 encodes a nerolidol/linalool synthase. transcripts of custs3-1, custs3-2 and custs4 were abundant in young fruit at 60 days after flowering (daf), flowers and leaves, respectively. treatments with xanthomonas citri subsp. citri (xcc), the causal agent of citrus canker and penicillium italicum (pi), the cause of post-harvest frui ...201425443842
decoctions from citrus reticulata blanco seeds protect the uroepithelium against escherichia coli invasion.traditional usage suggests citrus reticulata blanco seeds have beneficial effects against infection. the purpose of this study was to investigate the effect of citrus reticulata on the uroepithelium and to determine the mechanisms responsible for protection against urinary tract infection (uti).201324120518
[mollusicidal effects of some species of plants in hilly and mountainous areas].to valuate the mollusicidal effects of some plants cultivated in the hilly and mountainous areas on oncomelania hupensis and screen the plants against o. hupensis snails.201324024443
transcriptome and metabolome analyses of sugar and organic acid metabolism in ponkan (citrus reticulata) fruit during fruit maturation.ponkan (citrus reticulata blanco cv. ponkan) is an important mandarin citrus in china. however, the low ratio of sugars to organic acids makes it less acceptable for consumers. in this work, three stages (s120, early development stage; s195, commercial harvest stage; s205, delayed harvest stage) of ponkan fruit were selected for study. among 28 primary metabolites analyzed in fruit, sugars increased while organic acids in general decreased. rna-seq analysis was carried out and 19,504 genes were ...201525455100
development and reproduction of panonychus citri (prostigmata: tetranychidae) on different species and varieties of citrus plants.the species and varieties of citrus plants that are currently grown can favor the population growth of the citrus red mite panonychus citri (mcgregor) (prostigmata: tetranychidae) and alter the pest management programs in citrus groves. in this study we evaluated, in the laboratory, the development and reproduction of p. citri and estimated its life table parameters when reared on four varieties of citrus sinensis (l.) osbeck (valencia, pera, natal, and hamlin), one variety of citrus reticulata ...201526459376
pathogen infection drives patterns of nutrient resorption in citrus plants.nutrient resorption processes in the plants infected by pathogen remain poorly understood. huanglongbing (hlb) is a destructive disease of citrus. hlb-pathogen 'candidatus liberibacter asiaticus' grows specifically in the phloem of hosts and may cause problems in the plant vascular system after infection. therefore, it brings a great concern about the phloem nutrient transport and nutrient intra-cycling in hlb-affected plants. we investigated the effects of 'ca. l. asiaticus' infection on nitrog ...201526419510
a survey of plants responsible for causing irritant contact dermatitis in the amathole district, eastern cape, south africa.potentially harmful plants grow almost everywhere, hence, it is not practical to eradicate them all. however, a basic understanding of adverse cutaneous reactions and the common plants that cause each type can enable vulnerable individuals to discover the source of their dermatitis and thus prevent re-exposure. the aim of this study therefore, was to document the plants responsible for irritant contact dermatitis (icd) in the eastern cape, along with their respective irritants and clinical prese ...201425311272
effects of plant extracts on microbial population, methane emission and ruminal fermentation characteristics in in vitro.this study was conducted to evaluate effects of plant extracts on methanogenesis and rumen microbial diversity in in vitro. plant extracts (artemisia princeps var. orientalis; wormwood, allium sativum for. pekinense; garlic, allium cepa; onion, zingiber officinale; ginger, citrus unshiu; mandarin orange, lonicera japonica; honeysuckle) were obtained from the plant extract bank at korea research institute of bioscience and biotechnology. the rumen fluid was collected before morning feeding from a ...201225049630
parasitoids and hyperparasitoids (hymenoptera) on aphids (hemiptera) infesting citrus in east mediterranean region of turkey.the aphids, aphid parasitoids, and hyperparasitoids found in citrus orchards, the parasitoids' and hyperparasitoids' seasonal abundance, and the plant-aphid-parasitoid relationships in hatay, osmaniye, adana, and mersin provinces of the east mediterranean region of turkey are presented in the present 2-yr study. aphidius colemani viereck, binodoxys angelicae (haliday), and lysiphlebus confusus tremblay and eady (hymenoptera: braconidae: aphidiinae) were encountered as the most common parasitoids ...201425480969
comparative transcriptional survey between self-incompatibility and self-compatibility in citrus reticulata blanco.seedlessness is an excellent economical trait, and self-incompatibility (si) is one of important factors resulting in seedless fruit in citrus. however, si molecular mechanism in citrus is still unclear. in this study, rna-seq technology was used to identify differentially expressed genes related to si reaction of 'wuzishatangju' (citrus reticulata blanco). a total of 35.67gb raw rna-seq data was generated and was de novo assembled into 50,364 unigenes with an average length of 897bp and n50 val ...201728137595
in vitro physicochemical, phytochemical and functional properties of fiber rich fractions derived from by-products of six fruits.a comparative study was done on the health promoting and functional properties of the fibers obtained as by-products from six fruits viz., pomace of carambola (averrhoa carambola l.) and pineapple (ananas comosus l. merr), peels of watermelon (citrullus lanatus), burmese grape (baccurea sapida muell. arg) and khasi mandarin orange (citrus reticulata blanco), and blossom of seeded banana (musa balbisiana, abb). highest yield of fiber was obtained from burmese grape peel (bgpl, 79.94 ± 0.41 g/100  ...201627570274
heavy metals health risk assessment for population via consumption of food crops and fruits in owerri, south eastern, nigeria.this study assessed lead, cadmium, and nickel level in food crops, fruits and soil samples from ohaji and umuagwo and owerri in south eastern nigeria and estimated the potential health risks of metals. samples were washed, oven-dried at 70-80°c for 24 h and powdered. samples were digested with perchloric acid and nitric acid. metals were analysed with unicam atomic absorption spectrophotometer.201222853175
green synthesis of gold nanoparticles using citrus fruits (citrus limon, citrus reticulata and citrus sinensis) aqueous extract and its characterization.this study reports the biological synthesis of gold nanoparticles by the reduction of haucl(4) by using citrus fruits (citrus limon, citrus reticulata and citrus sinensis) juice extract as the reducing and stabilizing agent. a various shape and size of gold nanoparticles were formed when the ratio of the reactants were altered with respect to 1.0mm chloroauric acid solution. the gold nanoparticles obtained were characterized by uv-visible spectra, transmission electron microscopy (tem) and x-ray ...201323211617
protective effect of tangeritin in transgenic drosophila model of parkinson's disease.parkinson's disease (pd) is a neurodegenerative disorder caused due to the loss of dopaminergic neurons in substantia nigra region of midbrain. the disease is characterized by the accumulation of alpha-synuclein into depositions known as lewy bodies. till date there is no cure for pd but the limited number of medications may provide temporary relief from the pd symptoms. flavonoids are a group of polyphenols found in plants. the health benefits of flavonoids have been universally accepted. tange ...201727814588
evaluation of codon biology in citrus and poncirus trifoliata based on genomic features and frame corrected expressed sequence tags.citrus, as one of the globally important fruit trees, has been an object of interest for understanding genetics and evolutionary process in fruit crops. meta-analyses of 19 citrus species, including 4 globally and economically important citrus sinensis, citrus clementina, citrus reticulata, and 1 citrus relative poncirus trifoliata, were performed. we observed that codons ending with a- or t- at the wobble position were preferred in contrast to c- or g- ending codons, indicating a close associat ...201323315666
volatile constituents of wild citrus mangshanyegan (citrus nobilis lauriro) peel oil.volatiles of a wild mandarin, mangshanyegan (citrus nobilis lauriro), were characterized by gc-ms, and their aroma active compounds were identified by aroma extract dilution analysis (aeda) and gas chromatography-olfactometry (gc-o). the volatile profile of mangshanyegan was compared with those of other four citrus species, kaopan pummelo (citrus grandis), eureka lemon (citrus limon), huangyanbendizao tangerine (citrus reticulata), and seike navel orange (citrus sinensis). monoterpene hydrocarbo ...201222352344
reusable nanobiocatalysts for the efficient extraction of pigments from orange peel.nagpur mandarin orange (citrus reticulata) peels were subjected to treatment with nanobiocatalysts in the form of cellulase and pectinase immobilized magnetic nanoparticles (mnps). mnps (fe3o4) with average diameter in range of 40-90 nm were immobilized with cellulase and pectinase through aptes and glutaraldehyde. treatment followed by extraction into organic solvents resulted in 8-9 fold increase in extraction of carotenoidic pigments compared to use of free enzymes. optimum ph and temperature ...201627765971
inhibitory effects of four plants flavonoids extracts on fatty acid synthase.fatty acid synthase (fas) had been found overexpress and hyperactive in most cancers. pharmacological inhibitors of fas activity preferentially repress cancer cell proliferation and induce cancer cell apoptosis without affecting nonmalignant fibroblasts. these made fas an excellent drug target for cancer therapy. the activity of fas in 11 different kinds of cancer cells, including esophageal carcinoma (ec109, ec8712, h5e973), gastric carcinoma (n87, bgc823), lung carcinoma (a549, 95-d), hepatoma ...200925084411
quantification of pesticides in food crops using quechers approaches and gc-ms/ms.the analysis of pesticides in food products requires accurate measurements for which standardised protocols have been developed. this paper reports the validation of quechers (quick, easy, cheap, effective, rugged, and safe)-based methods applied to three different food samples (brown rice, red pepper and mandarin orange) analysed using gas chromatography-tandem mass spectrometry (gc-ms/ms). the validation of the analytical methods used to measure 113 pesticides, including various insecticides, ...201627619377
metals in agricultural produce associated with acid-mine drainage in mount morgan (queensland, australia).acid-mine drainage (amd) into the dee river from the historic gold and copper mine in mount morgan, queensland (australia) has been of concern to farmers in the area since 1925. this study sought to determine the levels of amd-related metals and sulfur in agricultural produce grown near the mine-impacted dee river, compare these with similar produce grown in reference fields (which had no known amd influence), and assess any potential health risk using relevant australian or us guidelines. analy ...201626979303
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