| isolation and characterization of fish scale collagen of higher thermal stability. | collagen is the most abundant protein found in animal body and widely used for biomedical and pharmaceutical applications. however, its applicability is severely limited due to high cost. fish processing waste, which otherwise cause serious environmental pollution, is a promising cost effective collagen source. in the present study, collagen was isolated from scales of labeo rohita (rohu) and catla catla (catla). it is first time that these species are used as sources of collagen. thermo-gravime ... | 2010 | 20116238 |
| behavioral and morphological endpoints: as an early response to sublethal malathion intoxication in the freshwater fish, labeo rohita. | a short-term definitive test by the static renewal bioassay method was conducted to determine the acute toxicity (lc(50)) of commercial-grade organophosphate insecticide, malathion (50% ec) on the freshwater fish, labeo rohita. carp fingerlings were exposed to different concentrations of malathion for 96 hours. the acute toxicity (lc(50)) of malathion was found to be 4.5 microg/l. one tenth (1/10, 0.45 microg/l) of the acute toxicity value was selected as the sublethal concentration for subacute ... | 2010 | 20307142 |
| indian carp (labeo rohita) gall bladder poisoning-report of four cases in a single family. | the ingestion of indian carp gallbladder may result in transient hepatitis with subsequent acute renal failure. this case series also illustrates the importance of understanding the use and potential serious complications of alternative medicines. so fish gallbladder poisoning should be considered in unexplained acute renal failure in chinese and asian patients. we report four family members who developed acute renal failure and toxic hepatitis at the same time following ingestion of raw indian ... | 2011 | 21207198 |
| two new species of myxobolus (myxozoa: myxosporea: bivalvulida) infecting indian freshwater fishes in punjab wetlands (india). | a survey of parasites of freshwater fishes in harike and ropar wetlands of punjab (india) revealed the presence of two new myxosporean species belonging to the genus myxobolus butschli 1882. spores of the first species, m. duodenalis sp. nov. parasitize the inner wall of the duodenum of wallago attu (bloch and schneider) (cypriniformis: cyprinidae) vern. mulli are characterized by a broad, pyriform shape with a blunt anterior end and measures 9.0 +ù 3.20 ++m. spore valves are thin, smooth, and s ... | 2011 | 21394535 |
| heavy metals in eight edible fish species from two polluted tributaries (aik and palkhu) of the river chenab, pakistan. | concentration of heavy metals (lead (pb), cadmium (cd), chromium (cr), and copper (cu)) was determined in the liver, gills, kidneys, and muscles of eight edible fish species (channa punctata, cirrhinus reba, labeo rohita, heteropneustes fossilis, mystus cavasius, oreochromis niloticus, puntius sophore, and wallago attu) from upstream and downstream zones of the nullah aik and palkhu tributaries of the river chenab located in the sialkot district known for its tanning industries worldwide. the pa ... | 2011 | 21424780 |
| isolation of enriched carp spermatogonial stem cells from labeo rohita testis for in vitro propagation. | the in vitro culture system for spermatogonial stem cells (sscs) is a powerful tool for exploring molecular mechanisms of male gametogenesis and gene manipulation. very little information is available for fish ssc biology. our aim was to isolate highly pure sscs from the testis of commercially important farmed carp, labeo rohita. the minced testis of l. rohita was dissociated with collagenase. dissociated cells purified by two-step ficoll gradient centrifugation followed by magnetic activated ce ... | 2011 | 21496900 |
| immunomodulating potency of lipopolysaccharides (lps) derived from smooth type of bacterial pathogens in indian major carp. | lipopolysaccharides (lps), the outer membrane of the gram-negative bacteria, are reported to stimulate the immunity of different vertebrates including fish. however, their potency and spectrum of actions often differ among different bacteria. in this study, effect of crude lps, derived from three species of smooth gram-negative bacterial fish pathogens viz. edwardsiella tarda, escherichia coli, and pseudomonas fluorescens, on certain innate immune parameters of indian major carp, labeo rohita wa ... | 2011 | 21507588 |
| physical, mechanical, and barrier properties of carp and mammalian skin gelatin films. | films of 0.11 to 0.13 mm thickness were prepared using gelatins from the skins of cultured freshwater carp species and mammalian gelatins viz., porcine and bovine skin gelatin. a comparative study was made on the physical, mechanical, and barrier properties of these films. the amino acid composition, gel strength, clarity, and gel setting point of the gelatins were also determined. carp skin gelatins had a lower imino acid content (19.16% to 20.86%) than mammalian skin gelatins (22.91% to 23.7%) ... | 2010 | 21535597 |
| high dietary protein combats the stress of labeo rohita fingerlings exposed to heat shock. | the amelioration effect of high dietary protein against stress was evaluated in labeo rohita fingerlings, exposed to heat shock. two hundred and forty fingerlings (6.57 ± 0.04 g, average weight ± se) were randomly distributed into 4 treatment groups, each with 4 replicates was fed with either of four diets containing different levels of protein (20, 30, 40 or 45%). water temperatures of all the treatments were within the range of 25.5-26.5°c throughout the experimental period of 30 days. after 3 ... | 2011 | 21573753 |
| water soaking and exogenous enzyme treatment of plant-based diets: effect on growth performance, whole-body composition, and digestive enzyme activities of rohu, labeo rohita (hamilton), fingerlings. | a 2 × 2 × 2 factorial experiment was conducted to delineate the main effect of water soaking of plant ingredients, phytase, cellulase, and their interactions on the growth and digestive enzyme activities of labeo rohita fingerlings. two basal diets were prepared using water-soaked (s) or unsoaked (us) plant-based ingredients. feed of us ingredients was supplemented with phytase (u kg(-1)) and cellulase (%) at the level of 0, 0 (c(us)); 500, 0 (t(1)); 0, 0.2 (t(2)); 500, 0.2 (t(3)), and feed of s ... | 2011 | 21611720 |
| physical mapping of 5s rdna in two species of knifefishes: gymnotus pantanal and gymnotus paraguensis (gymnotiformes). | physical mapping of 5s rdna in 2 species of knifefishes, gymnotuspantanal and g. paraguensis (gymnotiformes), was performed using fluorescence in situ hybridization with a 5s rdna probe. the 5s rdna pcr product from the genomes of both species was also sequenced and aligned to determine non-transcribed spacer sequences (nts). both species under study had different patterns of 5s rdna gene cluster distribution. while in the karyotype of g. pantanal two 5s rdna-bearing pairs were observed, the kar ... | 2011 | 21654160 |
| inhibition of atpase activity in the freshwater fish labeo rohita (hamilton) exposed to sodium cyanide. | present study concerns the effect of sodium cyanide on the indian major carp, labeo rohita. fishes were exposed to lethal (0.32?mg/l) and sublethal (0.064?mg/l) concentrations of sodium cyanide. the effect of intoxication was studied on na(+)k(+)atpase, mg(+2)atpase and ca(2+)atpase in various physiological tissues (gill, liver, and muscle) at the end of 1, 2, 3 and 4 days of lethal and 5, 10 and 15 days of sublethal exposure periods. sodium cyanide induced significant inhibitory effects on the ... | 2011 | 21777177 |
| molecular characterization of nucleotide binding and oligomerization domain (nod)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu (labeo rohita). | nucleotide-binding and oligomerization domain (nod)-2 is a cytoplasmic pattern recognition receptor (prr) and is a member of nod like receptor (nlr) family. it senses a wide range of bacteria and viruses or their products and is involved in innate immune responses. in this report, nod-2 gene was cloned and characterized from rohu (labeo rohita) which is highly commercially important fish species in the indian subcontinent. the full length rohu nod-2 (rnod-2) cdna comprised of 3176bp with a singl ... | 2011 | 21767564 |
| genetic divergence in wild population of labeo rohita (hamilton, 1822) from nine indian rivers, analyzed through mtdna cytochrome b region. | the present study examined partial cytochrome b gene sequence of mitochondrial dna for polymorphism and its suitability to determine the genetic differentiation in wild labeo rohita. the 146 samples of l. rohita were collected from nine distant rivers; satluj, brahmaputra, son, chambal mahanadi, rapti, chauka, bhagirathi and tons were analyzed. sequencing of 307 bp of cyto b gene revealed 35 haplotypes with haplotype diversity 0.751 and nucleotide diversity (p) 0.005. the within population varia ... | 2011 | 21748320 |
| modulation in the protein metabolism by subacute sodium cyanide intoxication in the freshwater fish, labeo rohita (hamilton). | the effects of exposure to one-third and one-fifth sublethal concentrations (0.106 and 0.064ôçëmg/l) of sodium cyanide on protein metabolism on freshwater carp, labeo rohita, was studied. three functionally different tissues, namely, the liver, muscle, and gills, were studied after 5, 10, and 15 days. exposures produced marked changes in protein metabolic profile in all tissues studied. these changes were more pronounced in the one-third sublethal concentration, suggesting a cumulative action of ... | 2011 | 21830916 |
| methyl mercury in fish-a case study on various samples collected from ganges river at west bengal. | this study investigated the presence of total mercury (hg) and organic mercury levels in the muscle of 19 common fresh water fish species captured from river ganges, west bengal, india. the total mercury level found in our study may not cause any toxic effect, but the methyl mercury (mehg) level in some freshwater fish species was surprisingly very high and toxically unacceptable. the results of mercury analysis in various specimens indicated that some fish muscles tended to accumulate high leve ... | 2011 | 21713467 |
| cloning of cdna and prediction of peptide structure of plzf expressed in the spermatogonial cells of labeo rohita. | the promyelocytic leukemia zinc finger (plzf) gene containing an evolutionary conserved btb (bric-a-brac/tramtrack/broad complex) domain plays a key role in self-renewal of mammalian spermatogonial stem cells (sscs) via recruiting transcriptional co-repressors. little is known about the function of plzf in vertebrate, especially in fish species. to gain better understanding of its role in fishes, we have cloned plzf from the testis of labeo rohita (rohu), a commercially important freshwater carp ... | 2010 | 21798210 |
| oxidative damage to lipids and proteins induced by aflatoxin b(1) in fish (labeo rohita)-protective role of amrita bindu. | in the present study, fish (labeo rohita) were treated with a single intraperitoneal administration of aflatoxin b(1) (afb(1)) (100++g/100gbw). the resultant oxidative damage to lipids (measured as conjugated diene and lipid peroxidation (lpo)) and proteins (protein carbonyl) in liver, kidney and brain at the end of 3rd and 6th day was assessed. our results showed that afb(1) induced a significant increase in conjugated diene formation and lpo not only in liver but also in kidney and brain. a pa ... | 2004 | 21782716 |
| selection for improved resistance to aeromonas hydrophila in indian major carp labeo rohita: survival and innate immune responses in first generation of resistant and susceptible lines. | selection for disease resistance in fish may be performed directly on basis of survival data obtained in controlled challenge trials, or indirectly using information from immunological or molecular markers linked to differential survival. in the present study, several key innate immune parameters were measured in aeromoniasis resistant and susceptible lines of rohu labeo rohita to assess their suitability as immune markers for use in indirect selection for increased resistance. experimental infe ... | 2011 | 21712094 |
| effects of dietary pyridoxine on haemato-immunological responses of labeo rohita fingerlings reared at higher water temperature. | a sixty-day feeding trial was carried out to elucidate the effect of dietary pyridoxine (pn) on hemato-immunological parameters in labeo rohita fingerlings exposed to an elevated temperature (et) of 33ôçâ-¦c. two hundred and seventy fingerlings (6.71ôçâ-¦ôçâ0.32ôçâg) were randomly distributed into six treatments in triplicates (15ôçâfish/tank). five iso-nitrogenous (356.3ôçâ-¦ôçâ2.7ôçâg/kg crude protein) purified diets were prepared with graded levels (0, 10, 50, 100 and 200ôçâmg/kg diet) of pn. ... | 2011 | 21692873 |
| Molecular characterization of toll-like receptor 2 (TLR2), analysis of its inductive expression and associated down-stream signaling molecules following ligands exposure and bacterial infection in the Indian major carp, rohu (Labeo rohita). | Toll-like receptors (TLRs) are one of the key components of innate immunity. Among various TLR types, TLR2 is involved in recognizing specific microbial structures such as peptidoglycan (PGN), lipoteichoic acid (LTA), zymosan etc., and after binding them it triggers myeloid differentiation primary response gene 88 (MyD88)-dependent signaling pathway to induce various cytokines. In this report, TLR2 gene was cloned and characterized in rohu (Labeo rohita), which is highly commercially important f ... | 2011 | 22173272 |
| Differential resistance to edwardsiellosis in rohu (Labeo rohita) families selected previously for higher growth and/or aeromoniasis-resistance. | Selection in fish for disease resistance is one of the most useful approaches to solve disease problems. Genetic variation in resistance to edwardsiellosis in fullsib families of rohu, Labeo rohita was investigated in the present study. A large variation in the susceptibility pattern (0 to 94.74 percent survival) against Edwardsiella tarda challenge was observed among 57 families. Additive genetic variation showed a heritability of 0.38?±?0.08 across the year-class survival. The apparent resista ... | 2011 | 22012677 |
| antigen adsorbed calcium phosphate nanoparticles stimulate both innate and adaptive immune response in fish, labeo rohita h. | calcium phosphate nanoparticles as an antigen/protein delivery was explored in a fish model labeo rohita h. s-layer protein (of aeromonas hydrophila) adsorbed on nano sized calcium phosphate particles elicited both innate and adaptive immune parameters which persisted up to 63 days of post immunization through parenteral immunization and gave cross protections. | 2011 | 21889128 |
| Dietary choline, betaine and lecithin mitigates endosulfan-induced stress in Labeo rohita fingerlings. | A five-week experiment was conducted to delineate stress-mitigating effects of three different methyl donors in Labeo rohita fingerlings subjected to endosulfan toxicity. Four iso-nitrogenous and iso-caloric feed were prepared with and without supplementation of methyl donors. The feed were basal or control diet (i.e., without methyl donor supplementation), feed supplemented with choline, feed supplemented with betaine and feed supplemented with lecithin. Two hundred and twenty-five fishes were ... | 2011 | 22160664 |
| study of the effects of the casein derived bitter tastant on the melanophores in milieu with the melatonin receptors. | the present study was undertaken to ascertain whether the casein derived bitter tastant cyclo (leu-trp) [clt] has an affinity or not for the particular receptors of the pineal hormone, melatonin, on the melanophores of a major carp labeo rohita (ham.). the bitter tastant clt, in the dose range of 3.34×10(-16) m to 3.34×10(-4) m, has induced an aggregatory effect but not in a dose dependent manner. binding of clt with the receptors may vary at different concentrations. denervation of the melanoph ... | 2011 | 21929292 |
| dose dependence specific and non-specific immune responses of indian major carp (l. rohita ham) to intraperitoneal injection of formalin killed aeromonas hydrophila whole cell vaccine. | specific and non-specific immune response to different doses of formalin killed whole cell vaccine of aeromonas hydrophila to indian major carp (labeo rohita) was evaluated in laboratory condition. three different doses (10(5) cfu/ml, 10(7) cfu/ml, 10(10) cfu/ml) were administered (0.2 ml/fish) intraperitoneally for 1 month. among the three doses, 10(10) cfu/ml elicited the highest antibody and protective response followed by the doses 10(7) cfu/ml and 10(5) cfu/ml. upon challenge with the virul ... | 2011 | 21901335 |
| Effect of alternate day feeding strategy of sub-optimal protein level on haemato-biochemical responses in Labeo rohita (Hamilton) juveniles. | A feeding trial was conducted for 60 days to delineate the effect of alternate day feeding strategy of sub-optimal protein level on haematological parameters, serum parameters and phagocyte respiratory burst activity (NBT) in Labeo rohita juveniles. One hundred and thirty-five fingerlings (1.87 ± 0.01-2.26 ± 0.05 g) were distributed in triplicate groups of each treatment, and fish were fed at 5% body weight daily. Three experimental isocaloric (401.32-410.28 kcal/100 g) diets of 30%, 25%, and 20 ... | 2011 | 21883499 |
| parasitic incidence in a cyprinid fish labeo rohita (ham.) at river song in doon valley (uttarakhand). | on examining 144 specimens of labeo rohita (ham.) for parasites occurrence; as many as 34 fish were found positive to harbour ciliophorans (trichodina sp. and ichthyophthirius multifilis fouquet, 1876), monogeneans (haplocleidus vachi tripathi, 1959 and dactylogyrus glossogobii jain, 1960), trematode (allocreadium mahaseri pandey, 1939), nematode (camallanus (zeylanema) anabantis pearse, 1933) and acanthocephalan (sachalinorhynchus sp). highest prevalence was shown by platyhelminthes (18.75%) fo ... | 2011 | 23542706 |
| two new species of myxobolus (myxozoa: myxosporea: bivalvulida) infecting an indian major carp and a cat fish in wetlands of punjab, india. | a survey of parasites of freshwater fishes in kanjali and harike wetlands of punjab (india) revealed the presence of two new myxosporean species belonging to the genus myxobolus bütschli (winter (ed): protozoa, 1882). spores of the first species, m. saugati sp. nov. parasitizing scales of labeo rohita (cypriniformis: cyprinidae) vern. rohu are characterized in having 'spores measuring 8.3 × 6.6 μm in size, oval to spherical in valvular view having rounded anterior and posterior ends; polar capsu ... | 2011 | 23024499 |
| observation on prevalence of ectoparasites in carp fingerlings in two districts of west bengal. | the present study has been carried out to find out the prevalence of ectoparasites of carp fingerlings during different months of the year 2008-2009. four groups of ectoparasites viz. myxozoan, ciliophoran, monogenean and crustacean were recorded from 400 fingerlings of rohu (labeo rohita), catla (catla catla), mrigal (cirrhinus mrigala), bata (labeo bata), common carp (cyprinus carpio) and lata (channa punctatus) collected from different ponds of nadia and hooghly district of west bengal from j ... | 2010 | 21526033 |
| record of the species of tripartiella (lom, 1959) from fishes of manipur. | survey on trichodinid ciliophorans from the fresh water fishes of manipur revealed three known species of the genus tripartiella from the gills of major carps labeo rohita (hamilton); cirrhinus mrigala (hamilton); catla catla and ciprinus carpio. these are redescribed in this communication. | 2012 | 23542123 |
| analysis of lactate and malate dehydrogenase enzyme profiles of selected major carps of wetland of calcutta. | the east calcutta wetland (ecw), a ramsar site in india, acts as the only sink for both city sewages as well as effluents from the surrounding small-scale industries and is alarmingly polluted with heavy metals. the three best edible major carp species rohu (labeo rohita,), catla (catla catla,) and mrigala (cirrhinus mrigala) were undertaken to monitor lactate dehydrogenase (ldh) and malate dehydrogenase (mdh) by cellulose acetate electrophoresis (cae) to assess the effects of pollutants, if any ... | 2012 | 23360005 |
| relationship between snail population density and infection status of snails and fish with zoonotic trematodes in vietnamese carp nurseries. | fish-borne zoonotic trematodes (fzt) are a food safety and health concern in vietnam. humans and other final hosts acquire these parasites from eating raw or under-cooked fish with fzt metacercariae. fish raised in ponds are exposed to cercariae shed by snail hosts that are common in fish farm ponds. previous risk assessment on fzt transmission in the red river delta of vietnam identified carp nursery ponds as major sites of transmission. in this study, we analyzed the association between snail ... | 2012 | 23285303 |
| computational analysis of transcriptome of indian major carp, labeo rohita (hamilton-buchanan, 1822) for functional annotation. | a total of 1671 ests of labeo rohita were retrieved from dbest database and analysed for functional annotation using various computational approaches. the result indicated 1387 non-redundant (184 contigs and 1203 singletons) putative transcripts with an average length of 542 bp. these 1387 transcript sequences were matched with refseq_rna, unigene and swiss-prot on high threshold cut-off for functional annotation along with help of gene ontology and ssrs markers. we developed extensive perl prog ... | 2012 | 23275698 |
| potential probiotic lactobacillus plantarum vsg3 improves the growth, immunity, and disease resistance of tropical freshwater fish, labeo rohita. | the study evaluated the effects of dietary doses of lactobacillus plantarum vsg3 on the growth performance, immunity, and disease resistance of labeo rohita juveniles against aeromonas hydrophila infection. fish (mean body weight 60 g) were fed with diet containing 0 (control), 10(6), 10(8), 10(10) cfu g(-1)l. plantarum vsg3 for 60 days. various growth and immune parameters were examined at 30 and 60 days of post-feeding. fish were challenged with a. hydrophila 60 days post-feeding and mortaliti ... | 2012 | 23274158 |
| a new myxosporean species myxobolus sclerii sp. nov. and one known species m. stomum ali et al. 2003 from two indian major carp fishes. | the present communication deals with description of one new species of myxobolus (myxozoa: myxosporea: bivalvulida), m. sclerii sp. nov. infecting eye ball of catla catla (hamilton) and redescription of m. stomum infecting scales of labeo rohita (hamilton), two major carps of kanjali and ropar wetlands respectively. spores of m. sclerii sp. nov. measure 7.9-9.5(8.7 ± 1.13) × 4.3-5.7(5 ± 0.98) μm in size. parietal folds absent. polar capsules two, equal and measuring 4-5.4(4.7 ± 0.98) × 1-2.6(1.8 ... | 2010 | 21526031 |
| thermosensitive ion channel trpv1 is endogenously expressed in the sperm of a fresh water teleost fish (labeo rohita) and regulates sperm motility. | sperm cells exhibit extremely high sensitivity in response to slight changes in temperature, osmotic pressure and/or presence of various chemical stimuli. in most cases throughout the evolution, these physico-chemical stimuli trigger ca ( 2+) -signaling and subsequently alter structure, cellular function, motility and survival of the sperm cells. few reports have recently demonstrated the presence of transient receptor potential (trp) channels in the sperm cells from higher eukaryotes, mainly fr ... | 2013 | 23912940 |
| expression analysis of heat shock protein genes during aeromonas hydrophila infection in rohu, labeo rohita, with special reference to molecular characterization of grp78. | heat shock protein (hsp) genes are stress-related genes that activate the host immune system during infection. hsp genes expression in fish, studied during bacterial infections, is mostly confined to hsp70 and hsp90. the present study is an expression analysis of seven hsp genes: apg2, hsp90, hsp70, glucose-regulated protein 78 (grp78), heat shock cognate 70 (hsc70), grp75, and hsp30 during aeromonas hydrophila infection in rohu, labeo rohita. forty-eight rohu juveniles were challenged with 3 × ... | 2014 | 25037476 |
| identification of deleterious mutations in myostatin gene of rohu carp (labeo rohita) using modeling and molecular dynamic simulation approaches. | the myostatin (mstn) is a known negative growth regulator of skeletal muscle. the mutated myostatin showed a double-muscular phenotype having a positive significance for the farmed animals. consequently, adequate information is not available in the teleosts, including farmed rohu carp, labeo rohita. in the absence of experimental evidence, computational algorithms were utilized in predicting the impact of point mutation of rohu myostatin, especially its structural and functional relationships. t ... | 2016 | 27019850 |
| molecular cloning and characterization of lrtlr4, analysis of its inductive expression and associated down-stream signaling molecules following lipopolysaccharide stimulation and gram-negative bacterial infection. | toll-like receptors (tlrs) play key roles in innate immunity from lower to higher vertebrates. among various tlr types, tlr4 was reported to recognize lps in higher vertebrates resulting in the activation of down-stream signaling pathway. except in some teleosts, function of tlr4 in most fish species including rohu (labeo rohita) a commercially important fish species in the south-east asian countries remained unknown. to investigate it, full-length cdna of labeo rohita tlr4 (lrtlr4) was cloned, ... | 2017 | 27838566 |
| dietary protein enhances non-specific immunity, anti-oxidative capability and resistance to aeromonas hydrophila in labeo rohita fingerlings pre-exposed to short feed deprivation stress. | present experiment was conducted to study the effect of dietary protein levels on growth, immunity and anti-oxidative status of labeo rohita fingerlings during feed deprivation followed by refeeding. fish (5.44 ± 0.10 g) were deprived of feed for 3 weeks and then re-fed to satiation for 5 weeks with one of the diets containing 25 (25p), 30 (30p), 35 (35p) or 40 (40p) percent crude protein (cp) level. in addition to these groups, a control group (c) was also maintained by feeding to satiation lev ... | 2016 | 27815199 |
| immunoglobulin (ig) d in labeo rohita is widely expressed and differentially modulated in viral, bacterial and parasitic antigenic challenges. | immunoglobulins (igs) play critical roles in protecting host against diverse pathogenic invasion and diseases. among all ig isotypes, igd is the most recently-evolved and enigmatic molecule detected in all vertebrates species except birds. in south-east asia, labeo rohita (rohu) is the leading candidate fish species for freshwater aquaculture, and this article describes about igd gene expression in rohu following viral, bacterial and parasitic antigenic challenges. the partial cdna (761bp) of la ... | 2016 | 27590429 |
| assessment of hemolytic activity, enzyme production and bacteriocin characterization of bacillus subtilis lr1 isolated from the gastrointestinal tract of fish. | in the present investigation, probiotic potential (antagonistic activity, enzyme production, hemolytic activity, biosafety, antibiotic sensitivity and bile tolerance level) of bacillus subtilis lr1 was evaluated. bacteriocin produced by the bacterial strain b. subtilis lr1 isolated from the gastrointestinal tract of labeo rohita was purified and characterized. the molecular weight of the purified bacteriocin was ~50 kda in 12 % native page and showed inhibitory activity against four fish pathoge ... | 2017 | 27590016 |
| role of dietary ginger zingiber officinale in improving growth performances and immune functions of labeo rohita fingerlings. | this study evaluated the effects of ginger (zingiber officinale) as a feeding supplement on the growth, skin mucus immune parameters, and cytokine-related gene expression of labeo rohita, and its susceptibility to aeromonas hydrophila infection. diets containing six different concentrations of dried ginger (0% [basal diet], 0.2% [g2], 0.4% [g4], 0.6% [g6], 0.8% [g8], and 1.0% [g10] were fed to fish (average weight: 12.3 g) for 60 days. growth parameters were examined at 30 and 60 days post-feedi ... | 2016 | 27574828 |
| b cell activating factor is induced by toll-like receptor and nod-like receptor-ligands and plays critical role in igm synthesis in labeo rohita. | b-cell activating factor (baff), an important member of the tumor necrosis factor superfamily, plays critical roles in the modulation of b-cell functions and enhancement of immune response in the host. like higher vertebrates, the important role of baff in boosting immune response against diverse pathogens was also envisaged in fishes. we therefore, studied baff in rohu (labeo rohita), a freshwater food fish species of highest economic importance in the indian subcontinent. full-length rohu-baff ... | 2016 | 27568001 |
| apolipoprotein a-i in labeo rohita: cloning and functional characterisation reveal its broad spectrum antimicrobial property, and indicate significant role during ectoparasitic infection. | apolipoprotein a-i (apoa-i) is the most abundant and multifunctional high-density lipoprotein (hdl) having a major role in lipid transport and potent antimicrobial activity against a wide range of microbes. in this study, a complete cds of 771 bp of labeo rohita (rohu) apoa-i (lrapoa-i) encoding a protein of 256 amino acids was amplified, cloned and sequenced. tissue specific transcription analysis of lrapoa-i revealed its expression in a wide range of tissues, with a very high level of expressi ... | 2016 | 27368542 |
| dietary administration of banana (musa acuminata) peel flour affects the growth, antioxidant status, cytokine responses, and disease susceptibility of rohu, labeo rohita. | to explore the feasibility of musa acuminata (banana) peels as a feed additive, effects of banana peel flour (bpf) on the growth and immune functions of labeo rohita were evaluated. diets containing five different concentrations of bpf (0% [basal diet], 1% [b1], 3% [b3], 5% [b5], and 7% [b7]) were fed to the fish (average weight: 15.3 g) for 60 days. the final weight gain and specific growth rate were higher (p < 0.05) in the b5 group. the most significant improvements in immune parameters such ... | 2016 | 27294156 |
| aeromonas hydrophila ompw plga nanoparticle oral vaccine shows a dose-dependent protective immunity in rohu (labeo rohita). | aeromonas hydrophila is a gram-negative bacterium that causes high mortality in different fish species and at different growth stages. although vaccination has significantly contributed to the decline of disease outbreaks in aquaculture, the use of oral vaccines has lagged behind the injectable vaccines due to lack of proven efficacy, that being from primary immunization or by use of boost protocols. in this study, the outer membrane protein w (ompw) of a. hydrophila was cloned, purified, and en ... | 2016 | 27258315 |
| detection of goldfish haematopoietic necrosis herpes virus (cyprinid herpesvirus-2) with multi-drug resistant aeromonas hydrophila infection in goldfish: first evidence of any viral disease outbreak in ornamental freshwater aquaculture farms in india. | this outbreak report details of a mortality event where cyprinid herpes virus-2 (cyhv-2) was detected in association with multidrug-resistant aeromonas hydrophila infection in goldfish, carassius auratus, from commercial farms. the goldfish exhibited large scale haemorrhages on the body, fins and gills, lepidorthosis, necrosed gills, protruded anus and shrunken eyes. white nodular necrotic foci in spleen and kidneys were noticed, along with necrosis and fusion of gill lamellae. transmission elec ... | 2016 | 27172876 |
| role of bacillus subtilis vsg4-derived biosurfactant in mediating immune responses in labeo rohita. | this study aimed to isolate biosurfactant from co2-sequestering bacillus subtilis vsg4 and to evaluate its immunostimulatory effect in labeo rohita fingerlings. fish were injected intraperitoneally (i.p.) with 0.1 ml of phosphate-buffered saline (pbs) containing the water-soluble fraction of purified biosurfactant at 50 (s50), 100 (s100), 200 (s200), or 300 (s300) μg ml(-1). fish injected with pbs served as controls. various immunological parameters, including immune-related gene expression, wer ... | 2016 | 27079425 |
| chlorophytum borivilianum polysaccharide fraction provokes the immune function and disease resistance of labeo rohita against aeromonas hydrophila. | the present study aimed to investigate the effects of chlorophytum borivilianum polysaccharide (cbp), as a dietary supplement administered at varying concentrations with feed (basal diet), on various cytokine-related responses in labeo rohita fingerlings. immune parameters and immune-related gene expressions were measured at 3rd, 4th, and 5th week after feeding. the results revealed that dietary administration of cbp at 0.2% and 0.4% for 4 weeks significantly upregulated serum lysozyme and phago ... | 2015 | 26649325 |
| four pro-inflammatory cytokines of rohu (labeo rohita) during early developmental stages, their tissue distribution and expression by leucocytes upon in-vitro stimulation. | cytokines are important components of both adaptive and innate immunity, and are required to initiate and regulate immune responses following infection. the ontogeny and tissue specific distribution of four pro-inflammatory cytokines, interleukin-6 (il-6), tumor necrosis factor α (tnf-α), il-8 and il-1β in rohu (labeo rohita), and their responses by leucocytes from anterior-kidney/head-kidney (hkls), spleen (spls) and peripheral blood (pbls) following stimulation with concanavalin a (cona), cona ... | 2015 | 26518505 |
| mitigation of immunosuppressive and oxidative stress effect of dietary gelatinized starch in labeo rohita fingerlings by elevation of rearing temperature within optimum range. | the present study was conducted to investigate the strategy to mitigate the immunosuppressive and oxidative stress effect of gelatinized starch in fingerling of labeo rohita. fingerlings were either maintained at ambient water temperature (26 °c) or exposed to 32 °c for one week and then subjected to 26 °c for four weeks. both groups were fed with isoproteinous (30% crude protein) diets containing gelatinized (g) or non-gelatinized (ng) starch. after 5 weeks of feeding trial, fingerlings were ch ... | 2015 | 26475365 |
| effect of cellular products of potential probiotic bacteria on the immune response of labeo rohita and susceptibility to aeromonas hydrophila infection. | in the present study, the immunological efficacy of cellular components from the potential probiotic bacteria bacillus subtilis vsg1, pseudomonas aeruginosa vsg2, and lactobacillus plantarum vsg3 was evaluated in labeo rohita fingerlings. fish were immunized intraperitoneally with 0.1 ml phosphate-buffer solution (pbs) containing 0.1 mg of any of the following cellular components: intercellular products (icps) of vsg1 (bs-icps), heat-killed whole cell products of vsg2 (pa-hkwcps), or icps of vsg ... | 2015 | 26282681 |
| effect of guava leaves on the growth performance and cytokine gene expression of labeo rohita and its susceptibility to aeromonas hydrophila infection. | the present study aimed to evaluate the effects of psidium guajava l. (guava) leaves on the growth and immune response of the fish species labeo rohita and its susceptibility to aeromonas hydrophila infection. diets containing five different concentrations of guava leaves (0% [basal diet], 0.1% [g1], 0.5% [g2], 1% [g3], and 1.5% [g4]) were fed to fish (average weight: 11.1 g) for 60 days. various growth and immune parameters were examined 60 days post-feeding. fish were challenged with a. hydrop ... | 2015 | 26093209 |
| ontogeny, tissue distribution and expression analysis of igz in rohu, labeo rohita in response to various stimuli. | this study reports presence of igz transcripts, its ontogeny, tissue distribution and expression following various stimulations/infections in rohu. the derived rohu igz sequence clustered together with igz of ctenopharyngodon idella and cyprinus carpio in phylogenetic analysis. igz expression was detected at early developmental stages with higher expression at 1 day post-fertilization, and higher in anterior kidney and lower in skin tissues of juveniles. an inductive expression of igz was observ ... | 2015 | 26055084 |
| isolation of potential probiotic bacillus spp. and assessment of their subcellular components to induce immune responses in labeo rohita against aeromonas hydrophila. | bacillus species isolated from the gut of healthy labeo rohita (hamilton) were screened for antibacterial activity against selected fish pathogens. among the isolates, kadr5 and kadr6 showed antibacterial activity, tolerated low ph and high bile concentrations and were susceptibility to various antibiotics. based on morphological and biochemical tests and 16s rrna gene analysis the probiotic strains kadr5 and kadr6 were identified as bacillus licheniformis and bacillus pumilus, respectively. the ... | 2015 | 25917974 |
| dietary inclusion of protease producing novel pontibacter spp. and bacillus megaterium as a probiotic enhances immune responses in labeo rohita. | abstract: this study stresses the key role which can be played by tannery fleshing (tf) hydrolyzing probiotic pontibacter spp. in aqua feed formulation and identifies the probiotic strains in the fish gut capable of enhancing the overall growth and immune responses. probiotics included are pontibacter species (pb) and bacillus megaterium (bm) wherein lactobacillus (lb) served as control. experimental diets includes tannery fleshing (tf1), tf+lb strain (tf2), tf+bm strain (tf3), tf+pb strain (tf4 ... | 2014 | 25911831 |
| parenteral immunization of pla/plga nanoparticle encapsulating outer membrane protein (omp) from aeromonas hydrophila: evaluation of immunostimulatory action in labeo rohita (rohu). | advanced vaccine research approaches needs to explore on biodegradable nanoparticles (nps) based vaccine carrier that can serve as antigen delivery systems as well as immuno-stimulatory action to induce both innate and adaptive immune response in fish. immunogenicity of pla and plga nps encapsulating outer membrane protein (omp) antigen of aeromonas hydrophila were evaluated through intra-peritoneal injection in fish, labeo rohita. antigen loaded pla-omp (223.5 ± 13.19 nm) and plga-omp (166.4 ± ... | 2015 | 25689492 |
| identification and immunogenic potential of b cell epitopes of outer membrane protein ompf of aeromonas hydrophila in translational fusion with a carrier protein. | aeromonas hydrophila, a ubiquitous and virulent bacterial pathogen, affects a variety of fishes, including labeo rohita. existing treatment strategies comprise antibiotic therapies and attenuated bacterial strain-based vaccines. no functional subunit vaccine has been available until now. given their key role in determining pathogenicity, outer membrane proteins have been successfully explored as potential vaccine candidates. we have devised a direct strategy for eliminating non-specific response ... | 2015 | 25636835 |
| in vitro evaluation of probiotic properties of lactic acid bacteria from the gut of labeo rohita and catla catla. | we report the evaluation of probiotic properties of potent lactic acid bacteria (lab) from the gut of freshwater fishes, labeo rohita and catla catla, for eventually developing probiotic strains for the prevention of bacterial infections in aquaculture and food preservation. five different lab strains were isolated and characterized for their probiotic properties. based on physiological, morphological and biochemical characteristics, three isolates from labeo rohita and two from catla catla were ... | 2015 | 25634754 |
| molecular characterization of interleukin 15 mrna from rohu, labeo rohita (hamilton): its prominent role during parasitic infection as indicated from infection studies. | interleukin 15 (il-15) is an important cytokine of fish immune system. sequence characterization of il-15 from rohu, labeo rohita revealed a mrna sequence of 1064 bp with coding sequence of 567 bp and signal peptide of 16 amino acids. there are four characteristic sequence features viz., presence of four out-of-frame aug initiation codons, four highly conserved cysteine residues, constitutive expression in all tissues and evolutionary similarity. the ontogeny study revealed maternal transfer of ... | 2015 | 25514374 |
| analysis of immune-related ests and differential expression analysis of few important genes in lines of rohu (labeo rohita) selected for resistance and susceptibility to aeromonas hydrophila infection. | a total of 137,629 contigs generated via de novo transcriptome assembly from resistant and susceptible lines of rohu (first generation) raised against aeromoniasis were further analyzed in terms of defence-related genes. out of 1,939 contigs showing homology to genes involved in immune processes, 1,866 were further categorised into different functional subgroups. comparative analysis revealed five genes for the first time in any carp species out of which apolipoprotein h, septin 4 isoform 3 and ... | 2014 | 25081649 |
| toll-like receptor 22 in labeo rohita: molecular cloning, characterization, 3d modeling, and expression analysis following ligands stimulation and bacterial infection. | toll-like receptors (tlrs) are a class of innate immune receptors that sense pathogens or their molecular signatures and activate signaling cascades to induce a quick and non-specific immune response in the host. among various types of tlrs, tlr22 is exclusively present in teleosts and amphibians and is expected to play the distinctive role in innate immunity. this report describes molecular cloning, three-dimensional (3d) modeling, and expression analysis of tlr22 in rohu (labeo rohita), the mo ... | 2014 | 25064133 |
| molecular cloning, sequencing and tissue-level expression of complement c3 of labeo rohita (hamilton, 1822). | complement component c3 plays a central role in all known complement activation pathways. in the present study, we cloned, sequenced and analyzed the full-length cdna sequence of labeo rohita complement c3 (lrc3). the expression pattern of complement c3 mrna in different tissues of healthy rohu and after challenge with aeromonas hydrophila were evaluated using real-time pcr. the lrc3 cdna sequence of rohu comprised of 5081 bp encoding a predicted protein of 1645 amino acids. the deduced amino ac ... | 2014 | 25038278 |
| a linkage map of transcribed single nucleotide polymorphisms in rohu (labeo rohita) and qtl associated with resistance to aeromonas hydrophila. | production of carp dominates world aquaculture. more than 1.1 million tonnes of rohu carp, labeo rohita (hamilton), were produced in 2010. aeromonas hydrophila is a bacterial pathogen causing aeromoniasis in rohu, and is a major problem for carp production worldwide. there is a need to better understand the genetic mechanisms affecting resistance to this disease, and to develop tools that can be used with selective breeding to improve resistance. here we use a 6 k snp array to genotype 21 full-s ... | 2014 | 24984705 |
| immune responses and protective efficacy of recombinant outer membrane protein r (rompr)-based vaccine of aeromonas hydrophila with a modified adjuvant formulation in rohu (labeo rohita). | despite the importance and success of developing a candidate vaccine against aeromonas hydrophila infection in fish, little is known about the molecular mechanisms of the vaccine-induced immunoprotection in indian major carp, labeo rohita, primarily due to lack of information on most of the immune related genes of the species. in this study, a novel candidate antigen recombinant outer membrane protein r (rompr) of a. hydrophila was evaluated as a vaccine candidate along with a modified adjuvant ... | 2014 | 24937805 |
| effect of dietary supplemented andrographolide on growth, non-specific immune parameters and resistance against aeromonas hydrophila in labeo rohita (hamilton). | the present study evaluated the effect of dietary andrographolide (ec 50%) on growth, non-specific immune parameters and disease resistance against aeromonas hydrophila infection in indian major carp, labeo rohita fingerlings. fishes were fed with formulated diet containing andrographolide as t0 (0.00%), t1 (0.05%), t2 (0.10%), t3 (0.20%), t4 (0.40%) and t5 (0.80%) for 42 days. fishes were challenged with a. hydrophila 42 days post feeding and relative percentage survival (rps) was recorded over ... | 2013 | 23973382 |
| application of sensory and microbial analysis to assess quality of fish in siliguri city of west bengal, india. | sensory and certain microbial analyses were applied to assess the quality of raw fish sold at a market in siliguri cityof west bengal, india. in regular surveys undertaken during june to august 2008, a particular fish species was randomly selected, its source was noted and a sensory analysis, the quality index method (qim) was applied to assess its quality raw fish samples were also collected and a small quantity (about 1 g) of scales oran upper layer of the skin surface (forscale-less fish samp ... | 2010 | 21387907 |
| ontogeny and tissue-specific expression of innate immune related genes in rohu, labeo rohita (hamilton). | the innate immune response in fish represents an early and rapid defense against pathogens. the present study aims at looking into ontogeny of innate immune system in the teleost, labeo rohita using rt-pcr based approach. total rna extracted from unfertilized and fertilized eggs, and hatchlings (hatched at 28 ± 2 °c) at 0, 1, 3, 6, 12, 24 h, and 3, 7, 16, 21, 31 days post-fertilization were subjected to rt-pcr using self-designed or earlier published primers to amplify some innate immune relevan ... | 2011 | 21362484 |
| comparative susceptibility of carp fingerlings to lernaea cyprinacea infection. | study was conducted to find out the comparative susceptibility of fingerlings of seven species of carps (labeo fimbriatus, l. rohita, l. calbasu, catla catla, ctenopharyngodon idella, cyprinus carpio and hypophthamichthys molitrix) grown under both mono and polyculture to lernaea cyprinacea infection. under monoculture, c. carpio, l. rohita and l. calbasu, did not acquire lernaea infection and were thus considered resistant, whereas c. idella, h. molitrix, c. catla and l. fimbriatus were suscept ... | 2010 | 21255932 |
| effect of surfactants on phosphatase level of fresh water fish labeo rohita. | alterations in the activity of enzymes acid phosphatase (e.c.3.1.3.2) and alkaline phosphatase (ec 3.1.3.1) in organs such as liver, gills and muscle of rohu following its exposure to surfactants viz. ctab, sds and triton x-100 were analyzed. different levels of exposure were given depending on the lc50 value of the surfactant used. also, the influence of age and weight of the organisms was tested simultaneously. the activity of acp in the tissues of fish exposed to all the three surfactants sho ... | 2010 | 21186708 |
| monoclonal antibodies to snakehead, channa striata immunoglobulins: detection and quantification of immunoglobulin-positive cells in blood and lymphoid organs. | snakehead channa striata is an important freshwater food fish in many southeast asian countries. three monoclonal antibodies (c9, c10 and d10) were developed against purified serum immunoglobulins of channa striata (cs-ig) and characterized. c9 and d10 mabs were specific to heavy chain, while c10 mab detected only unreduced cs-ig in western blotting. in competitive elisa, c9 and c10 mabs were specific to c. striata ig and showed no cross reactivity with serum ig of other fish species i.e. channa ... | 2010 | 21167285 |
| application of comet assay in the study of dna damage and recovery in rohu (labeo rohita) fingerlings after an exposure to phorate, an organophosphate pesticide. | labeo rohita (rohu) fingerlings were exposed to different concentrations (0.001, 0.002 and 0.01 ppm) of phorate, an organophosphate pesticide; samplings were done at 24, 48, 72 and 96 h. the study was carried out to evaluate tissue specific genotoxic effects produced by phorate, on three different tissue systems and to assess dna repair response in fish. results of tissue specific dna damage experiments showed low baseline damage in blood cells followed by gill and liver cells in control individ ... | 2010 | 21153054 |
| the ontogeny of mauthner cells in the brain of labeo rohita as revealed by nadph-d and nnos immunohistochemistry. | nitric oxide (no) is well demonstrated to act as a neuronal messenger in neurotransmission in vertebrate animals. we are for the first time reporting nicotinamide adenine dinucleotide phosphate-diaphorase (nadph-d) and neuronal nitric oxide synthase (nnos) in the mauthner cells (mc) of the carp, labeo rohita brain during post-embryonic development. the nadph-d and nnos-positive mc were seen intensely labeled in all young stages on either side of the ventricle in the hindbrain. in adult, these ce ... | 2010 | 21132510 |
| haemato-immunological and stress responses of labeo rohita (hamilton) fingerlings: effect of rearing temperature and dietary gelatinized carbohydrate. | a feeding trial of 70-days was carried out to study the haemato-immunological and stress responses of labeo rohita fingerlings reared at two water temperatures [ambient (amb) - 27 °c and 32 °c] fed with graded levels of gelatinized corn carbohydrate (gc). two hundred and sixteen fingerlings were randomly distributed into six treatment groups in triplicate. three semi-purified diets were prepared containing 30% crude protein with graded levels of gc 40%, 50% and 58%. the six treatment groups were ... | 2010 | 21114548 |
| higher water temperature enhances dietary carbohydrate utilization and growth performance in labeo rohita (hamilton) fingerlings. | a 60-day experiment was conducted to delineate the effect of three dietary levels of gelatinized carbohydrate (gc) on growth, nutrient-utilization and body composition of labeo rohita fingerlings (avg. wt 6.5 ± 0.3 g) reared at two water temperatures (ambient-at (26 ± 0.8 °c) and 32 °c). two hundred and sixteen fingerlings were randomly distributed into six treatments in triplicates. three semi-purified isonitrogenous diets were prepared with graded levels of gc viz. d(1) : 40%, d(2) : 50% and d ... | 2010 | 21114547 |
| application of calcined waste fish (labeo rohita) scale as low-cost heterogeneous catalyst for biodiesel synthesis. | this paper explores the feasibility of converting waste rohu fish (labeo rohita) scale into a high-performance, reusable, low-cost heterogeneous catalyst for synthesis of biodiesel from soybean oil. the thermo-gravimetric analysis (tga) and x-ray diffraction (xrd) analysis revealed that a significant portion of the main component of fish scale i.e. hap (hydroxyapatite) could be transformed into β-tri-calcium phosphate when calcined above 900°c for 2 h. scanning electron microscopy (sem) morpholo ... | 2010 | 21094040 |
| expression of immune-related genes in rohu labeo rohita (hamilton) by experimental freshwater lice argulus siamensis (wilson) infection. | the crustacean ectoparasite, argulus poses one of the major threats to carp culture due to absence of any suitable control measure. the study was undertaken to determine the expression of immune-related genes in three major immunocompetent organs viz., kidney, skin and liver of rohu (labeo rohita) during experimental freshwater lice argulus siamensis infection. results showed that the expression of tlr 22-like, lysozyme g and β2-microglobulin genes in kidney was significantly (p ≤ 0.05) down-reg ... | 2010 | 21051150 |
| simple gas chromatographic method for the quantification of total cholesterol in fish meats. | six fish species-cirrhinus mrigala (morakhi), labeo rohita (danbhro), catla catla (thalli), wallago attu (jarko), ctenopharyngodon idella (grass), and cyprimus carpio (gulfam)--commonly found in the pakistani indus river, were selected for fat and cholesterol evaluation. fat content was quantified by the folch method and cholesterol content was determined by a simple gc method. the application of the proposed method to quantify cholesterol content revealed variations among the six breeds evaluat ... | 2010 | 20922959 |
| studies on lipid profiles and fatty acid composition of roe from rohu (labeo rohita) and murrel (channa striatus). | the lipid profiles and fatty acid composition of roes from two common fresh water fish viz., rohu (labeo rohita) and murrel (channa striatus) have been studied. the dehydrated fish egg was ground to fine powder and extracted with chloroform/methanol (2:1, v/v) to recover the total lipid in 20.2 and 22.7% on dry weight basis. the fat free meal was found to contain 70 and 58% protein in rohu and murrel, respectively. the total lipids were separated into neutral lipids, glycolipids and phospholipid ... | 2010 | 20877143 |
| yeast extract, brewer's yeast and spirulina in diets for labeo rohita fingerlings affect haemato-immunological responses and survival following aeromonas hydrophila challenge. | a feeding trial was conducted for 60days to study the immunomodulatory role of three different immunostimulants yeast extract (ye), brewer's yeast (by) and spirulina (sp) in labeo rohita fingerlings. four hundred and fifty fingerlings (avg. wt 3.35±0.15g) were randomly distributed in ten treatments and fed with either of ten iso-nitrogenous and iso-caloric semi-purified diets, prepared with three incremental levels (1%, 2% and 4%) of different immunostimulants except the control. growth paramete ... | 2010 | 20825959 |
| haemato-immunology and histo-architectural changes in labeo rohita fingerlings: effect of dietary aflatoxin and mould inhibitor. | a feeding trail of 60 days was conducted for delineating the effect of dietary aflatoxin (afb1) with or without supplementation of a mixture of mould inhibitor (0.25% clove oil + 0.32% sodium propionate) on haematology, respiratory burst activity and histology of labeo rohita fingerlings. three hundred and sixty fishes (avg. wt. 1.48-1.54 g) were randomly distributed into eight treatment groups. eight experimental diets with four different levels of aflatoxin (0, 10, 20 and 40 ppb) with or witho ... | 2011 | 20803068 |
| biochemical and stress responses of rohu labeo rohita and mrigal cirrhinus mrigala in relation to acclimation temperatures. | the biochemical and stress responses of two indian major carps, rohu labeo rohita and mrigal cirrhinus mrigala were studied after acclimating them to four preset temperatures (26, 31, 33 and 36 degrees c) for 30 days. the blood glucose and liver glycogen levels showed an inverse trend in both the species and were significantly different in l. rohita at higher temperatures. the decrease in the liver glycogen level of c. mrigala, however, was not significant. plasma cortisol levels increased signi ... | 2009 | 20735648 |
| development and characterization of three new diploid cell lines from labeo rohita (ham.). | development of cell lines from fish for identifying the pathogenesis of viral diseases and for vaccine production against viral and bacterial diseases is imperative where they are of commercial importance. three new diploid fish cell lines (rf, rh, and rsb) were developed from fin, heart, and swim bladder of an indian major carp, labeo rohita, commonly called rohu. all the cell lines were optimally maintained at 28 degrees c in leibovitz-15 medium supplemented with 10% fbs. the propagation of rh ... | 2010 | 20730759 |
| effect of withania somnifera (l. dunal) root as a feed additive on immunological parameters and disease resistance to aeromonas hydrophila in labeo rohita (hamilton) fingerlings. | the present study evaluated the efficacy of dietary doses of withania somnifera (ashwagandha) root powder on immunological parameters and disease resistance against the bacterial pathogen aeromonas hydrophila infections in indian major carp, labeo rohita fingerlings. fishes were fed with dry diet containing 0 gkg(-1) (control), 1 gkg(-1) (t(1)), 2 gkg(-1) (t(2)) and 3 gkg(-1) (t(3)) w. somnifera root powder for 42 days. immunological (nbt level, phagocytic activity, total immunoglobulin and lyso ... | 2010 | 20580830 |
| assessing metal, protein, and dna profiles in labeo rohita from the indus river in mianwali, pakistan. | this study assessed metals in water and different tissues of labeo rohita and the impact of these metals on dna and proteins as biomarkers of gills and muscles of these fish from three different polluted sites (reference or low = kw, medium = ch and high = sk) of the indus river, pakistan. the mn, pb, cu, zn, hg, and cr levels in water, gills, liver, muscles, and skin of these fish were compared with the international permissible levels. all metals except pb and hg in water were within the accep ... | 2011 | 20461548 |
| fish-borne zoonotic trematodes in cultured and wild-caught freshwater fish from the red river delta, vietnam. | nam dinh province in red river delta, vietnam, is an endemic area for the human liver fluke clonorchis sinensis, but little is known about its occurrence in the fish intermediate host in this region. a cross-sectional study was carried out to identify fish-borne zoonotic trematodes (fzt) species diversity and to estimate the prevalence and infection densities of fzt in three major types of fish: cultured species and self-recruiting species from family ponds and wild-caught species from irrigatio ... | 2010 | 20370427 |
| inhibitory effect of pistia tannin on digestive enzymes of indian major carps: an in vitro study. | aquatic weeds are one of the major unconventional feed ingredients tested for aquafeed formulation. tannin content in the water lettuce, pistia, has been quantified (26.67 mg g(-1); dry weight) and graded levels of which (12.5-200 μg) have been incorporated in the reaction mixtures to evaluate any change in the in vitro activity of the principal digestive enzymes from the three indian major carps (imc), namely rohu (labeo rohita), catla (catla catla) and mrigala (cirrhinus mrigala). result of th ... | 2010 | 20369287 |
| kinetic study of hepatic β-glucuronidase in the indian major carp, labeo rohita (hamilton). | liver is the metabolic factory and contains several valuable enzymes that catalyze biochemical reactions. β-glucuronidase is one of the well-known lysozymes that participates in the carbohydrate metabolism in the tissues of various vertebrates. in the present study, an attempt was made to study the kinetic properties of hepatic β-glucuronidase of the indian major carp (imc), labeo rohita. it was observed that the enzyme activity increased largely at ph 5 (0.1 m acetate buffer) when exposed to 38 ... | 2010 | 20336485 |
| toxicity of crude extracellular products of aeromonas hydrophila on rohu, labeo rohita (ham.). | in the present study the haemolytic and proteolytic activity of extracellular products (ecp) secreted from aeromonas hydrophila (cahh14 strain) were studied with respect to temperature and different time of incubation as well as its lethal toxicity on rohu, labeo rohita. the strain was isolated from catla catla (showing abdominal dropsy symptom) collected from the pond of central institute of freshwater aquaculture (cifa), bhubaneswar, india and was characterized on the basis of biochemical test ... | 2011 | 23024416 |
| studies on trichodinid and chilodonellid ciliophorans (protozoa: ciliophora) in the indian freshwater and estuarine fishes with description of trichodinella sunderbanensis sp. nov. and trichodina nandusi sp. nov. | a comprehensive icthyoparasotological survey among estuarine and freshwater fishes of west bengal, india revealed trichodinella sunderbanensis sp. nov. and trichodina acuta lom, 1961 from an estuarine fish mystus gulio (hamilton, 1822) from the vidyadhari river; trichodina nandusi sp. nov. from a freshwater fish, nandus nandus (hamilton-buchanan); chilodonella hexasticha (kiernik, 1909) kahl, 1931 from freshwater fishes labeo rohita (hamilton, 1822); and cyprinus carpio linnaeus, 1758 from the r ... | 2012 | 23269510 |
| characterization of the ceruloplasmin gene and its potential role as an indirect marker for selection to aeromonas hydrophila resistance in rohu, labeo rohita. | ceruloplasmin is an acute phase protein found to be activated by the host immune system during stress conditions. the ceruloplasmin gene has been reported in several teleosts and here we characterize the gene and test its association with resistance to aeromonas hydrophila in rohu, labeo rohita. a ceruloplasmin mrna sequence of 3355 base pairs (bp) was derived (genbank id: jx010736). the coding sequence (cds) comprised of 3276 bp that coded for 1092 amino acids. alignment results showed the grea ... | 2013 | 23481214 |
| effect of dietary supplementation with achyranthes aspera seed on larval rohu labeo rohita challenged with aeromonas hydrophila. | larval rohu labeo rohita were fed four different diets: three of the diets contained achyranthes aspera (prickly chaff-flower) seeds at 0.10% (d1), 0.25% (d2), or 0.50% (d3); the fourth diet was a control diet (d4; no a. aspera supplementation). after 70 d, the rohu were injected intraperitoneally with live aeromonas hydrophila. mortality of fish was recorded for 7 d. in the d4 group, the first mortality was observed within 12 h of exposure, whereas in the d1-d3 treatment groups, mortality was f ... | 2012 | 23025591 |
| recombinant aeromonas hydrophila outer membrane protein 48 (omp48) induces a protective immune response against aeromonas hydrophila and edwardsiella tarda. | the gene coding for an outer membrane protein omp48 of aeromonas hydrophila isolated from an infected fish was cloned and sequenced. analysis of nucleotide sequence showed the omp48 gene to be an adhesin encoding a protein of 426 amino acids with high identity to the omp48 gene of aeromonas veronii, another fish pathogen. the gene belonged to the maltoporin group of porins and had high similarity to lamb porins of a. hydrophila, aeromonas salmonicida and vibrio parahaemolyticus. the expressed pu ... | 2012 | 22564558 |
| molecular cloning and characterization of nucleotide binding and oligomerization domain-1 (nod1) receptor in the indian major carp, rohu (labeo rohita), and analysis of its inductive expression and down-stream signalling molecules following ligands exposure and gram-negative bacterial infections. | nucleotide binding and oligomerization domain-1 (nod1) is a cytoplasmic pattern recognition receptor (prr), and is a member of the nod-like receptor (nlr) family. it senses a wide range of bacteria and viruses or their products, and plays a key role in inducing innate immunity. in this report, nod1 gene was cloned and characterized in rohu (labeo rohita), a fish species of highest commercial importance in the indian subcontinent. the full-length rohu nod1 (rnod1) cdna comprised of 3168 bp with a ... | 2012 | 22530240 |
| effects of dietary supplementation of potential probiotic pseudomonas aeruginosa vsg-2 on the innate immunity and disease resistance of tropical freshwater fish, labeo rohita. | the effects of dietary pseudomonas aeruginosa vsg-2 supplementation on innate immunity and protection against aeromonas hydrophila infection were evaluated in labeo rohita. fish were fed for 60 days with control diet or 3 experimental diets containing p. aeruginosa vsg-2 at 10(5), 10(7), and 10(9) cfu g(-l), respectively. various innate immune parameters were examined at 30 and 60 days post-feeding. fish were challenged with a. hydrophila 60 days post-feeding and mortalities were recorded over 1 ... | 2012 | 22465361 |
| expressed sequences and polymorphisms in rohu carp (labeo rohita, hamilton) revealed by mrna-seq. | expressed genes and polymorphisms were identified in lines of rohu labeo rohita selected for resistance or susceptibility to aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis. all animals were grown in a common environment and rna from ten individuals from each line pooled for illumina mrna-seq. de novo transcriptome assembly produced 137,629 contigs with 40× average coverage.forty-four percent of the assembled sequences were annotated with gene names and ontology terms. ... | 2012 | 22298294 |
| antagonistic activity of cellular components of potential probiotic bacteria, isolated from the gut of labeo rohita, against aeromonas hydrophila. | the objective of this study was to characterise the antagonistic activity of cellular components of potential probiotic bacteria isolated from the gut of healthy rohu (labeo rohita), a tropical freshwater fish, against the fish pathogen, aeromonas hydrophila. three potential probiotic strains (referred to as r1, r2, and r5) were screened using a well diffusion, and their antagonistic activity against a. hydrophila was determined. biochemical tests and 16s rrna gene analysis confirmed that r1, r2 ... | 2011 | 26781682 |
| characterization of bacillus spp. from the gastrointestinal tract of labeo rohita--towards to identify novel probiotics against fish pathogens. | the aim of the present study is to screen and characterize endogenous microbiota bacillus spp. from the gastrointestinal (gi) tract of labeo rohita in order to evaluate their probiotic attributes. a total of 74 isolates from the gi of l. rohita were evaluated for their antimicrobial properties by agar well-diffusion method against fish pathogens. based on the better antibacterial features, three isolates (kadr1, kadr3, and kadr4) were selected for further delineation. the three selected isolates ... | 2015 | 25274116 |