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the influence of auxins on the biosynthesis of isoprene derivatives in callus cultures of vaccinium corymbosum var. bluecrop.callus cultures of vaccinium corymbosum var. bluecrop were optimized for their isoprene derivatives production by supplementing schenk-hildebrandt (sh) medium with constant concentration of kinetin (2.32 microm) and two different amounts of selected auxins. every auxin, except for iba, used in 10-time higher concentration (2,4d, naa, iaa, noa) stimulated biosynthesis of beta-sitosterol and inhibited triterpene synthesis. quantitative analysis of isoprene derivatives in callus biomass collected o ...201316989318
shelf-life extension of highbush blueberry using 1-methylcyclopropene stored under air and controlled atmosphere.the potential of 1-methylcyclopropene for controlling ripening in 'lateblue' blueberry fruit was explored. after harvest, blueberry fruits were exposed to 1-mcp (0.3 and 0.6μll(-1)). after treatment, samples were stored in air at 0°c for 35days and in a controlled atmosphere (3kpa o2+11kpa co2) for 60days. quality parameters were monitored (weight loss, total soluble solids content, titratable acidity, firmness, anthocyanin content, phenolic content, total antioxidant capacity). blueberries trea ...201125213961
organ-specific distribution of phenolic compounds in bilberry (vaccinium myrtillus) and 'northblue' blueberry (vaccinium corymbosum x v. angustifolium).blueberries and bilberries are recognized as some of the best sources of flavonoids, especially anthocyanins. the contents of flavonoids (anthocyanins, proanthocyanidins, flavonols) and hydroxycinnamic acids in the flower, fruit skin and pulp, leaf and rhizome of bilberry and the blueberry cultivar 'northblue' were analyzed using high-performance liquid chromatography combined with diode-array detection. the most striking difference in the fruits was the predominance of hydroxycinnamic acids in ...200826050178
postharvest shelf life extension of blueberries using a biodegradable package.small berries are commonly packaged and sold to consumers in vented petroleum-based clamshell containers. biodegradable and compostable packages may be used as an alternative package to reduce waste generation and landfill disposal. in addition, the current clamshell container design does not allow the development of a modified atmosphere that could prolong berry shelf life. thus, in this study, a non-ventilated biodegradable container was evaluated as a possible alternative to the containers no ...200826050174
fruit quality, antioxidant capacity, and flavonoid content of organically and conventionally grown blueberries.the effect of cultivation practices on fruit quality and antioxidant capacity in highbush blueberries var. bluecrop (vaccinium corymbosum l.) was evaluated from random samples of commercial late harvest fields in new jersey. results from this study showed that blueberry fruit grown from organic culture yielded significantly higher sugars (fructose and glucose), malic acid, total phenolics, total anthocyanins, and antioxidant activity (orac) than fruit from the conventional culture. in organicall ...200818590274
major differences observed in transcript profiles of blueberry during cold acclimation under field and cold room conditions.our laboratory has been working toward increasing our understanding of the genetic control of cold hardiness in blueberry (vaccinium section cyanococcus) to ultimately use this information to develop more cold hardy cultivars for the industry. here, we report using cdna microarrays to monitor changes in gene expression at multiple times during cold acclimation under field and cold room conditions. microarrays contained over 2,500 cdna inserts, approximately half of which had been picked and sing ...200716953429
evaluation of vaccinium spp. for illinoia pepperi (hemiptera: aphididae) performance and phenolic content.host acceptance and population parameters of the aphid illinoia pepperi (macgillivray) (hemiptera: aphididae) were measured on highbush blueberry, vaccinium corymbosum l. 'elliott', and the wild species vaccinium boreale hall and aalders, vaccinium tenellum aiton, vaccinium pallidum aiton, vaccinium hirsutum buckley, vaccinium myrsinites lamarck, and vaccinium darrowi camp. after 24 h of exposure, significantly fewer aphids remained in contact with v. boreale and v. hirsutum compared with v. cor ...200616937706
isoprene derivatives from the leaves and callus cultures of vaccinium corymbosum var. bluecrop.the phytochemical analysis of vaccinium corymbosum var bluecrop leaves and callus biomass revealed ursolic acid, oleanolic acid, alpha-amyrin and beta-amyrin in both plant materials. beta-sitosterol was determined only in callus biomass. the structure of isolated compounds was elucidated by tlc co-chromatography with standards and with spectroscopic methods (1h nmr, 13c nmr, ei-ms).201216022493
the change of total anthocyanins in blueberries and their antioxidant effect after drying and freezing.this study examined the effects of freezing, storage, and cabinet drying on the anthocyanin content and antioxidant activity of blueberries (vaccinium corymbosum l). fresh samples were stored for two weeks at $\5^\circ$ c while frozen samples were kept for up to three months at $-20^\circ$ c. there were two drying treatments, one including osmotic pretreatment followed by cabinet drying and the other involving only cabinet drying. total anthocyanins found in fresh blueberries were $7.2 \pm 0.5$ ...200415577185
effect of high-oxygen atmospheres on blueberry phenolics, anthocyanins, and antioxidant capacity.the influence of high oxygen concentrations on total phenolic, total anthocyanin, individual phenolic compounds, and antioxidant capacity (measured as oxygen radical absorbance capacity, orac) in highbush blueberry fruit (vaccinium corymbosum l. cv. duke) was investigated. freshly harvested blueberries were placed in jars ventilated continuously with air or with 40, 60, 80, or 100% o(2) at 5 degrees c for up to 35 days. samples were taken initially and at 7-day intervals during storage. whereas ...200314611188
anthocyanin and proanthocyanidin content in selected white and red wines. oxygen radical absorbance capacity comparison with nontraditional wines obtained from highbush blueberry.antioxidant capacity, as measured by oxygen radical absorbance capacity (orac(pe)), total phenolic, total and individual anthocyanins, and proanthocyanidin fraction contents were evaluated in red and white wines from grapes. a comparison in terms of antioxidant capacity is made with nontraditional wines made from highbush blueberry. blueberries are among fruits that are best recognized for their potential health benefits. in red wines, total oligomeric proanthocyanidin content, including catechi ...200312903941
oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries.the antioxidant activity of phenolics in fruits of blueberry (vaccinium corymbosum cv. sierra), cranberry (vaccinium macrocarpon cv. ben lear), wild chokeberry (aronia melanocarpa), and lingonberry (vaccinium vitis-idaea cv. amberland) was determined in this study. the phenolic constituents and contents among the different berries varied considerably. anthocyanins were found to be the main components in all these berries. chlorogenic acid in blueberry, quercetin glycosides in cranberry and lingo ...200312517117
optimization of pheromone deployment for effective mating disruption of oriental beetle (coleoptera: scarabaeidae) in commercial blueberries.cost is a potential limiting factor in the adoption of mating disruption to control oriental beetle, anomala orientalis (waterhouse) (coleoptera: scarabaeidae). a 3-yr study was conducted in 1-ha blueberry, vaccinium corymbosum l., plots to test the possibility of lowering cost by reducing the number of point-source dispensers and pheromone [(z)-7-tetradecen-2-one] concentrations, while maintaining mating disruption. trap shutdown, as measured by the disruption index (di), caged females, and sen ...200919449647
monitoring and temperature-based prediction of the whitemarked tussock moth (lepidoptera: lymantriidae) in blueberry.larvae of the whitemarked tussock moth, orgyia leucostigma (j.e. smith) (lepidoptera: lymantriidae), defoliate and contaminate blueberries, vaccinium corymbosum l., in eastern north america, but infestations are often not detected until economic damage has been caused. to improve monitoring techniques and understand the phenology of the whitemarked tussock moth in blueberry, we compared four trap types and determined temperature-based phenology of this pest over two growing seasons. large delta ...200919449644
identification of dehydrin-like proteins responsive to chilling in floral buds of blueberry (vaccinium, section cyanococcus).the level of three major polypeptides of 65, 60, and 14 kd increased in response to chilling unit accumulation in floral buds of a woody perennial, blueberry (vaccinium, section cynaococcus). the level of the polypeptides increased most dramatically within 300 h of chilling and decreased to the prechilling level with the initiation of budbreak. cold-hardiness levels were assessed for dormant buds of vaccinium corymbosum and vaccinium ashei after different chilling treatments until the resumption ...19948016270
growth characteristics and ion contents of non-selected and salt-selected callus lines of highbush blueberry (vacdnium corymbosum) cultivars blue crop and denise blue.non-selected and sodium chloride selected callus lines of vacdnium corymbosum l.cv blue crop and cv. denise blue were grown on media supplemented with 0-100 mm nacl. for both cultivars, fresh weight and dry weight yields were greater in selected lines on all levels of nacl. selected lines of blue crop displayed better growth than selected lines of denise blue at most concentrations of nacl. internal na(+) and cl(-) concentrations in selected and non-selected lines of both cultivars increased as ...199024226601
early-acting inbreeding depression and reproductive success in the highbush blueberry, vaccinium corymbosum l.tetraploid vaccinium corymbosum genotypes exhibit wide variability in seed set following self- and cross-pollinations. in this paper, a post-zygotic mechanism (seed abortion) under polygenic control is proposed as the basis for fertility differences in this species. a pollen chase experiment indicated that self-pollen tubes fertilize ovules, but are also 'outcompeted' by foreign male gametes in pollen mixtures. matings among cultivars derived from a pedigree showed a linear decrease in seed numb ...199024226746
production and viability of unreduced gametes in triploid interspecific blueberry hybrids.three triploid (2n=3x=36) blueberry hybrids were obtained by hand-pollinating approximately 7,000 flowers of tetraploid highbush blueberry cultivars (based on vaccinium corymbosum l.) with pollen from the diploid species v. elliottii chapm. meiotic analysis of these triploids revealed trivalents, bivalents and univalents in all metaphase i cells, with lagging chromosomes evident at anaphase i. pollen of the three triploids was mostly aborted and did not stain with acetocarmine. however, the thre ...198824232275
in vitro attempts to overcome the cross-incompatibility between vaccinium corymbosum l. and v. elliottii chapm.seed was readily obtained from v. corymbosum zygotes using embryo rescue techniques, even when embryos were cultured at proembryonic stages. best in vitro seed development was obtained when ovules were cultured attached to placental tissues. successful fruit and seed development in culture occurred only when the fruit was cut longitudinally or when the basal portion of the fruit was removed previous to plating. addition of various vitamins, amino acids, and growth regulators to the nutrient medi ...198524254018
anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: vaccinium, rubus, and ribes.fruits from 107 genotypes of vaccinium l., rubus l., and ribes l., were analyzed for total anthocyanins (acy), total phenolics (tph), and antioxidant capacities as determined by oxygen radical absorbing capacity (orac) and ferric reducing antioxidant power (frap). fruit size was highly correlated (r = 0.84) with acy within vaccinium corymbosum l., but was not correlated to acy across eight other vaccinium species, or within 27 blackberry hybrids. certain vaccinium and ribes fruits with pigmented ...200211804523
native vaccinium spp. and gaylussacia spp. infested by rhagoletis mendax (diptera: tephritidae) in the great lakes region: a potential source of inoculum for infestation of cultivated blueberries.in this study, we addressed the question of whether or not native stands of blueberry (vaccinium spp.) and/or huckleberry (gaylussacia spp.) support populations of blueberry maggot, rhagoletis mendax curran, in the great lakes region. infestation of commercial blueberries by the blueberry maggot, r. mendax, is a serious problem in many areas where blueberries are grown. in the past 10-20 yr, commercial bighbush blueberry, vaccinium corymbosum l., production has expanded into places such as south ...200111777039
evaluation of various deployment strategies of imidacloprid-treated spheres in highbush blueberries for control of rhagoletis mendax (diptera: tephritidae).biodegradable, ammonium-baited spheres treated with the neonicotinoid insecticide provado (imidacloprid) at 2% (ai) were evaluated for controlling blueberry maggot flies, rhagoletis mendax curran. three strategies for sphere deployment in highbush blueberries, vaccinium corymbosum l., were compared with untreated control plots in 1999 and once again compared against control plots and organophosphate insecticide sprays in 2000. the patterns of sphere deployment were as follows: (1) perimeter depl ...200111561850
oxygen radical absorbance capacity (orac) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry.antioxidant capacity, as measured by oxygen radical absorbance capacity (orac), and total phenolic and total anthocyanin contents were evaluated in fruit tissues of 87 highbush blueberry (vacciniumcorymbosum l.) and species-introgressed highbush blueberry cultivars. orac and phenolic levels were evaluated in leaf tissues of the same materials. average values for orac, phenolics, and anthocyanins in fruit were 15.9 orac units, 1.79 mg/g (gallic acid equivalents), and 0.95 mg/g (cyanidin-3-glucosi ...200111368580
effect of pollen load size and source (self, outcross) on seed and fruit production in highbush blueberry cv. 'bluecrop' (vaccinium corymbosum; ericaceae).reproductive fitness of a plant is ultimately determined by both number and quality of seed offspring. this is determined by sexual selection of pollen microspores and ovules during pollination and fertilization. these processes may include pollen competition and seed abortion, which reduce the number of microspores and ovules available for final seed production. thus, even an excess of pollen microspores to ovules does not result in fertile seeds equal to ovule number. we investigated pollen re ...200011080108
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