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natural anaplasmataceae infection in rhipicephalus bursa ticks collected from sheep in the french basque country.rhipicephalus bursa is one of 79 species of the genus rhipicephalus in the family of ixodidae. in this study, we investigated anaplasmataceae bacteria associated with r. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as r. bursa, all females were partially engorged. we found that 50% of the ticks were positive in the initial anaplasmataceae qpcr screening. bacterial species ...201727666778
a survey of ixodid tick species and molecular identification of tick-borne pathogens.this study was undertaken in two different climatic areas of turkey to determine the presence of tick-borne pathogens of medical and veterinary importance. the ticks were removed from humans, pooled according to species and developmental stages, and analyzed by pcr, reverse line blot (rlb) and sequencing. of the 2333 removed ticks from 10 species, 1238 (53.06%) were obtained from the arid cold zone, and the remaining 1095 (46.93%) were obtained from the humid zone. the removed ticks were identif ...201424424312
bacterial pathogens and endosymbionts in ticks.ticks collected from goats in northern greece were tested for the presence of tick-borne bacteria. among adult ticks, 37 (57.8%) were rhipicephalus bursa, 11 (17.2%) dermacentor marginatus, 10 (15.6%) ixodes ricinus, 3 (4.7%) rhipicephalus sanguineus sensu lato and 2 (3.1%) haemaphysalis parva; one (1.6%) rhipicephalus spp. tick was nymph. rickettsia monacensis, rickettsia massilae, anaplasma phagocytophilum and anaplasma platys were detected in i. ricinus and rh. bursa ticks. a variety of coxie ...201727686386
molecular evidence for bacterial and protozoan pathogens in hard ticks from romania.the aim of the present study was to provide a preliminary insight into the diversity of tick-borne pathogens circulating at the domestic host-tick interface in romania. for this, feeding and questing ticks were analyzed by real-time polymerase chain reaction (pcr) for the presence of anaplasma phagocytophilum, anaplasma platys, ehrlichia canis, borrelia burgdorferi sensu latu, and by pcr and subsequent sequencing for rickettsia spp., babesia spp. and theileria spp. a total of 382 ticks, encompas ...201323428204
a survey of ixodid ticks feeding on cattle and prevalence of tick-borne pathogens in the black sea region of turkey.the study reports the frequency of infestation and the prevalence of tick-borne pathogens in feeding adult ticks detached from cattle in two climatic zones of the black sea region of turkey. a total of 2160 adult ticks were collected during 2007-2008. of these, 1062 were randomly selected, divided into 224 pools, and tested for the presence of bovine theileria, babesia, and anaplasma species. eleven tick species were recognized on cattle in the study. hyalomma marginatum was widely disrubuted in ...201222365338
pcr screening of tick-borne agents in sensitive conservation areas, southeast portugal.the southeast region of portugal, particularly the guadiana valley, is currently the reintroduction territory of lynx pardinus (iberian lynx), one of the most endangered felids in the world that is only found in the iberian peninsula. over the last century, populations have declined, placing l. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. among the aspects taken into account in the establishment of new populations is the sanitary status of the ...201727894847
molecular characterization of babesia and theileria species in ticks collected in the outskirt of monte romano, lazio region, central italy.in 2012-2013, an investigation was carried out in the viterbo province, lazio region, on ticks and tick-borne apicomplexan protozoa of the babesia and theileria genera. this followed the reporting of high density of ticks by soldiers operating in a military shooting range, and the signaling by owners and local veterinary authorities of several cases of babesiosis among cattle.201728361802
molecular identification of theileria and babesia in ticks collected from sheep and goats in the black sea region of turkey.a molecular survey was undertaken in the black sea region of turkey to determine the presence of theileria and babesia species of medical and veterinary importance. the ticks were removed from sheep and goats, pooled according to species and locations, and analyzed by pcr-based reverse line blot (rlb) and sequencing. a total of 2241 ixodid ticks belonging to 5 genus and 12 species were collected and divided into 310 pools. infection rates were calculated as the maximum likelihood estimation (mle ...201525260692
molecular and serological detection of theileria equi and babesia caballi infection in horses and ixodid ticks in iran.equine piroplasmosis is a hemoprotozoan tick-borne disease with worldwide distribution that is caused by theileria equi and babesia caballi. however, the geographical distribution of equine piroplasmosis in iran is unknown. the aim of the current study was to determine the causative agents and vector ticks of equine piroplasmosis in horses in the north khorasan province. in the year 2011, 100 horses were randomly selected from 14 villages. blood samples and ixodid ticks were collected and examin ...201424556274
prevalence and genetic diversity of piroplasm species in horses and ticks from tunisia.the genetic diversity and prevalence of babesia and theileria species in the equine population of tunisia were studied using reverse line blot (rlb) hybridization on blood samples and unfed adult ticks collected from apparently healthy horses from three bioclimatic zones in tunisia. piroplasms were identified in 13 of 104 of the horse blood samples analyzed (12.5%) and five genotype groups were identified: theileria equi group a (nine animals, 8.7%), group c (one animal, 1.0%) and group d (three ...201323542456
molecular detection of theileria spp. and babesia spp. in sheep and ixodid ticks from the northeast of iran.theilerioses and babesioses are important diseases in iranian sheep. the present study was undertaken to identify and classify/specify theileria spp. and babesia spp. in sheep and vector ticks. investigation was carried out from 2009 to 2011 in the khorasan razavi province, iran. in total, 302 sheep originating from 60 different flocks were clinically examined and their blood collected. in addition, from the same flocks, ixodid ticks were sampled. stained blood smears were microscopically examin ...201322924924
molecular evidence for trans-stadial and transovarial transmission of babesia occultans in hyalomma marginatum and rhipicephalus turanicus in turkey.a total of 1064 adult ticks identified as hyalomma marginatum (n=965), rhipicephalus turanicus (n=86), haemaphysalis inermis (n=9) and rhipicephalus bursa (n=4) along with 20 egg masses derived from h. marginatum (n=18) and r. bursa were screened for babesia occultans. the ticks were combined into 328 pools and infection rates were calculated as the maximum likelihood estimation (mle) with 95% confidence intervals (ci). of these pools, 23 (7%) were positive for b. occultans, and the overall mle ...201425002306
molecular and parasitological survey of ovine piroplasmosis, including the first report of theileria annulata (apicomplexa: theileridae) in sheep and goats from turkey.blood and tick samples were collected from 333 apparently healthy sheep and 257 goats as well as 10 sheep exhibiting clinical signs of babesiosis in adana, gaziantep, and adiyaman provinces in southern turkey. fully engorged female ticks were selected and maintained in an incubator until they oviposited. the tick carcasses and their egg masses were examined. piroplasms compatible with babesia spp. and theileria spp. were observed in both symptomatic and asymptomatic small ruminants. genomic dna ...201728082649
molecular and parasitological survey of ovine piroplasmosis, including the first report of theileria annulata (apicomplexa: theileridae) in sheep and goats from turkey.blood and tick samples were collected from 333 apparently healthy sheep and 257 goats as well as 10 sheep exhibiting clinical signs of babesiosis in adana, gaziantep, and adiyaman provinces in southern turkey. fully engorged female ticks were selected and maintained in an incubator until they oviposited. the tick carcasses and their egg masses were examined. piroplasms compatible with babesia spp. and theileria spp. were observed in both symptomatic and asymptomatic small ruminants. genomic dna ...201627559124
quantitative analysis of babesia ovis infection in sheep and ticks.a quantitative pcr, based on the gene encoding babesia ovis surface protein d (bospd) was developed and applied to investigate the presence of babesia ovis (b. ovis) in its principal vector, the tick rhipicephalus bursa (r. bursa), and in the ovine host. quantification of b. ovis in experimentally-infected lambs showed a sharp increase in parasitemia 10-11 days in blood-inoculated and adult tick-infested lambs, and 24 days in a larvae-infested lamb. a gradual decrease of parasitemia was observed ...201627084469
molecular detection of theileria, babesia, and hepatozoon spp. in ixodid ticks from palestine.ixodid ticks transmit various infectious agents that cause disease in humans and livestock worldwide. a cross-sectional survey on the presence of protozoan pathogens in ticks was carried out to assess the impact of tick-borne protozoa on domestic animals in palestine. ticks were collected from herds with sheep, goats and dogs in different geographic districts and their species were determined using morphological keys. the presence of piroplasms and hepatozoon spp. was determined by pcr amplifica ...201626969489
molecular detection of babesia ovis in sheep and ticks using the gene encoding b. ovis surface protein d (bospd).the gene encoding babesia ovis surface protein d (bospd) was cloned from b. ovis cdna library. this gene encodes a polypeptide chain of 155 amino acids, including a predicted 22 amino acid signal peptide. sequence analysis of the bospd suggested that it is a surface protein with no known domains. blast analysis followed by multiple alignments showed four orthologs from other apicomplexan species and suggested that bospd is specific for b. ovis. bospd-based pcr was then developed to specifically ...201526391822
transmission of babesia ovis by different rhipicephalus bursa developmental stages and infected blood injection.in this report, the transmission efficacy of babesia ovis, the principal causative agent of ovine babesiosis, was studied by infestation of lambs with different rhipicephalus bursa stages or by injection of infected blood. infected blood injection induced acute babesiosis in splenectomized lambs, while only mild clinical signs were observed in intact animals. both splenectomized and intact lambs developed high antibody titer, detectable for at least 180 days post infection. infestation of splene ...201626253782
[molecular-genetic characterization of the bhanja virus (bhav) and the razdan virus (razv) (bunyaviridae, phlebovirus) isolated from the ixodes ticks rhipicephalus bursa (canestrini and fanzago, 1878) and dermacentor marginatus (sulzer, 1776) in transcaucasus].two bunyaviruses, bhanja (bhav, leiv-az1818) isolated from the ixodes ticks rhipecephalus bursa (canestrini and fanzago, 1878) in azerbaijan (1973) and razdan (razv; strain leiv-arm2741) isolated from the dermacentor marginatus (sulzer, 1776) ticks in armenia (1972), were de novo sequenced (on the illumina platform). the amino acid identity between these viruses proteins were 95.8% (rdrp, l-segment), 90.3% (gngc, m-segment), and 92.5% (n, s-segment). thus, razv was classified to bhav group. gngc ...201624354060
coxiella-like endosymbiont associated to the "anatolian brown tick" rhipicephalus bursa in southern italy.several different ticks have been reported to harbor microbes related to coxiella burnetii, the agent of the q fever. rhipicephalus bursa is an important vector of tick-borne diseases in livestock in mediterranean area; it is also abundant in ovi-caprine farms with c. burnetii infection, in southern italy. 60 females of rh. bursa (15 pools) and 40 their eggs (2 pools) were screened for c. burnetii by a conventional pcr targeting the insertion sequence is1111 and by loop mediated isothermal ampli ...201626428855
molecular (ticks) and serological (humans) study of crimean-congo hemorrhagic fever virus in the iberian peninsula, 2013-2015.crimean-congo hemorrhagic fever (cchf) is a viral disease, mainly transmitted through tick bite, of great importance in public health. in spain, crimean-congo hemorrhagic fever virus (cchfv) was detected for the first time in 2010 in hyalomma lusitanicum ticks collected from deer in cáceres. the aim of this study was to investigate the presence of cchfv in ticks from cáceres, and from other spanish areas, and to evaluate the presence of antibodies against the virus in individuals exposed to tick ...201728291670
hard ticks (ixodidae) and crimean-congo hemorrhagic fever virus in south west of iran.ticks are vectors of some important arthropod-borne diseases in both fields of veterinary and medicine, such as lyme, tularemia, rocky mountain spotted fever, and some types of encephalitis as well as crimean congo hemorrhagic fever (cchf). iran is known as one of the main foci of cchf in west of asia. this study was conducted in darrehshahr county because of the development of animal husbandry in this area to detect the fauna and viral infection of the hard ticks of livestock. a cross-sectional ...201525796025
a novel ap92-like crimean-congo hemorrhagic fever virus strain, greece.ticks were collected from various regions of northern greece and tested for the presence of crimean-congo hemorrhagic fever virus (cchfv) rna. human and animal sera were collected in the regions where cchfv-positive ticks were detected, and they were tested for the presence of igg antibodies against the virus. a cchfv strain was detected in rhipicephalus bursa ticks collected from sheep in kastoria regional unit, differing by 9.7% at the nucleotide level from the ap92 strain, which was isolated ...201424953797
first detection of ehrlichia canis in rhipicephalus bursa ticks in sardinia, italy.canine monocytic ehrlichiosis is a common and widespread disease. this disease is present in sardinia year-round because the temperate weather on the island permits the survival of many types of tick vectors. a total of 35 ticks was collected from goats in south-eastern sardinia, italy, and tested for ehrlichia dna using polymerase chain reaction and dna sequencing. this study provides the first evidence of the presence of ehrlichia canis in rhipicephalus bursa ticks. the data presented here inc ...201223140896
rickettsia slovaca from dermacentor marginatus ticks in sardinia, italy.nineteen ticks belonging to the species dermacentor marginatus, rhipicephalus bursa, and haemaphysalis sulcata were collected from wild animals (wild boar, deer, and mouflon) in south-western sardinia, italy. five d. marginatus ticks from wild boar were pcr-positive when analyzed using glta-specific and ompa-specific primers, leading to the identification and first isolation in cell culture of rickettsia slovaca, the causative agent of tick-borne lymphadenopathy (tibola), on the island of sardin ...201223140897
identification of tick species and disseminate pathogen using hemolymph by maldi-tof ms.matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is increasingly emerging tool for identification of arthropods including tick vectors using whole or body part of specimens. the challenges of the present study were to assess maldi-tof ms profiling for the both identification of tick species and rickettsia spp. in infected ticks using hemolymph as protein mixture.201526051210
molecular detection of theileria spp. in sheep and vector ticks in the north khorasan province, iran.a survey was carried in north khorasan province, iran in 2010-2011, designed to identify theileria spp. infections of both sheep and ticks. the tick species were also examined. ninety sheep from different flocks were clinically examined, and blood samples and ixodid ticks were collected. light microscopy of blood smears revealed theileria spp. infection in 37 (41.1 %), while 74 (82.2 %) of blood samples were positive using semi-nested pcr. theileria ovis, theileria lestoquardi, and mixed infecti ...201322791188
a molecular study of tick-borne haemoprotozoan parasites (theileria and babesia) in small ruminants in northern tunisia.in this study, the frequency of theileria and babesia species in sheep and goats was assessed via reverse line blotting (rlb). a total of 263 apparently healthy sheep and goats, from 16 randomly selected flocks located in 9 localities situated in 3 bioclimatic zones in tunisia, were investigated for the blood protozoans. rlb hybridization with polymerase chain reaction detected only theileria ovis in sheep and goats, accounting for 22.4% (95% confidence interval [ci]: 17.6-27.1%) positive sample ...201324029713
prevalence of ixodid ticks on cattle and sheep northeast of iran.a survey was carried out to investigate the prevalence of hard tick species (acari: ixodidae) on cattle and sheep north of iran. the aim of study was to determine the prevalence of hard ticks on cattle and sheep in the mountainous areas of golestan province and their geographical distribution. a total of 26 ticks were collected from 22 infested cattle and 26 ticks were collected from 12 infested sheep during activating seasons of ticks in 2013-2014. the species collected from cattle and sheep we ...201627605782
[distribution of tick species spread in kütahya vicinity].this study was performed to determine the tick species infested to the cattle and small ruminants in kütahya province and their seasonal activity and prevalence.201526081885
preliminary investigation on tick fauna in the neighborhood of tarquinia, lazio, italy.this study represents a preliminary inquiry on tick fauna composition carried out in maremma, central italy, where very few data are available.201525857387
species diversity and abundance of ticks in three habitats in southern italy.a 2-year study was conducted from march 2010 to march 2012 in a forested area in southern italy to evaluate the species diversity and abundance of free-living ticks in 3 different habitats: (i) a meadow habitat within an enclosure inhabited by roe deer (capreolus capreolus); (ii) a man-made trail located in a high-altitude, forested area; and (iii) a grassland near a house inhabited by 3 people. in total, 10,795 ticks were collected. ixodes ricinus was the most abundant species (69.0%), followed ...201323403153
long term study of ixodid ticks feeding on red deer (cervus elaphus) in a meso-mediterranean climate.red deer (cervus elaphus l.) are very valuable in trophy-hunting but also contribute to the preservation of natural areas. they are affected by many parasites and pathogens, including hard ticks that are not only important parasites themselves but can also act as vectors and/or reservoirs of pathogens. tick phenology is complex insofar as population dynamics depend on environmental conditions, vegetation, host availability and their own intrinsic characteristic. ticks were collected monthly from ...201626715543
ectoparasite load in the crested porcupine hystrix cristata linnaeus, 1758 in central italy.the crested porcupine hystrix cristata is a large body-sized rodent, occurring in europe only in the italian peninsula, where it may have been introduced in early medieval times. its parasite fauna is currently poorly known and limited to few anecdotal observations. we have analyzed the ectoparasite load of 165 crested porcupines from tuscany and latium (central italy). both captured and road-killed individuals were checked for fleas and ticks. overall, only 39 porcupines were infested by four s ...201525773184
a survey of ectoparasite infestation in dogs in tehran, iran.this survey was conducted to identify and estimate the frequencies of ectoparasites of dogs in tehran, iran. a total of 143 dogs attended at the small animal hospital of the veterinary school, the university of tehran, were examined for the presence of ectoparasites and dermatological lesions. ectoparasite specimens and blood samples were sent to parasitology and hematology laboratories, respectively. ticks were the most frequent ectoparasite (36.4%, 52/143), followed by fleas (29.4%, 42/143), m ...201223070452
long-term ecological study of host-seeking adults of hyalomma lusitanicum (acari: ixodidae) in a meso-mediterranean climate.from january 2007 to december 2014, three representative meso-mediterranean bioclimatic environment types were sampled monthly using blanket-dragging techniques to determine the tick abundance rate. hyalomma lusitanicum koch, 1844 was the most prevalent species (96.58%) followed by dermacentor marginatus sulzer, 1776; rhipicephalus pusillus gil collado, 1936; and rhipicephalus bursa canestrini and fanzago, 1878. h. lusitanicum adults begin questing activity around march, numbers rising quickly r ...201626477051
seasonal dynamics of tick species in an urban park of rome.regular collections were obtained in the natural reserve of the insugherata of rome during 2011 in order to obtain the tick species composition and the respective seasonal dynamics of the area. a total of 325 ticks was collected in selected sites by means of drag sampling. among the identified species, rhipicephalus turanicus was the most abundant (72.3%), followed by ixodes ricinus (19.7%), dermacentor marginatus (6.5%), haemaphysalis punctata (1.2%), and rhipicephalus bursa (0.3%). r. turanicu ...201324140501
molecular epizootiology and diagnosis of porcine babesiosis in sardinia, italy.the recent characterization of the 18s ribosomal rna (rrna) of a pathogenic babesia species in a domestic sow paved the way for establishing diagnostic and epidemiological tools for porcine babesiosis. here, we developed the first specific babesia sp. suis pcr, and we applied this test to a panel of samples collected from animals living in a typical mediterranean environment (sardinia, italy), including domestic pigs, wild boars, and ticks. in domestic pigs, pcr coupled with sequencing revealed ...201425325315
molecular investigation and phylogeny of anaplasmataceae species infecting domestic animals and ticks in corsica, france.corsica is a french island situated in the mediterranean sea. the island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. the family anaplasmataceae is a member of the order rickettsiales; it includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia. anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera anaplasma and ehrlichia. the a ...201728645313
crimean-congo hemorrhagic fever virus in ticks collected from livestock in albania.albania is a balkan country endemic for crimean-congo hemorrhagic fever (cchf). it was shown previously that cchf virus (cchfv) sequences from albanian patients cluster into europe 1 clade. aim of the present study was to test for cchfv ticks collected in several regions of albania, and to determine the genetic lineage(s) of the cchfv strains in relation with their geographic distribution. a total of 726 ticks (366 hyalomma marginatum, 349 rhipicephalus bursa and 11 rhipicephalus sanguineus) col ...201728827176
crimean-congo hemorrhagic fever virus in ticks in turkey: a broad range tick surveillance study.in turkey, crimean-congo hemorrhagic fever (cchf) outbreaks started in northern regions in 2002. human cases still continue to increase and the disease spreads in many other provinces of turkey. the ecological role of the vector tick species occurring in turkey is not certain exactly. therefore, we planned a broad range tick study in three different ecological and geographical areas extending from the west black sea regions down to the central anatolia. the aim of this study was to determine and ...201728433738
babesia, theileria, and hepatozoon species in ticks infesting animal hosts in romania.babesia spp., theileria spp., and hepatozoon spp. are tick-transmitted apicomplexan parasites that cause several important diseases in animals. to increase current knowledge about the diversity of tick-transmitted pathogens in romania, we investigated the occurrence of babesia spp., theileria spp., and hepatozoon spp. in a wide range of tick species infesting animal hosts. we collected 852 ticks from 10 different animal species from 20 counties in romania. the assessment was based on detection o ...201728676905
partial characterization of tick-borne encephalitis virus isolates from ticks of southern ukraine.tick-borne encephalitis (tbe) is the most common tick-borne viral infection in eurasia; thousands of human cases are annually reported from several european countries. several tick species are vectors of the tick-borne encephalitis virus (tbev), while tbe appears to be spreading from the eurasian continent westward to europe. fifteen study sites were chosen from five territories of southern ukraine, including odessa, mykolaiv, kherson oblast, the autonomous republic of crimea, and sevastopol. ti ...201728654319
molecular detection of crimean-congo hemorrhagic fever virus in ticks, greece, 2012-2014.crimean-congo hemorrhagic fever virus (cchfv) is transmitted to humans mainly through the bite of infected ticks. in greece, only one clinical case has been observed, in 2008, but the seroprevalence in humans is relatively high (4.2%). to have a first insight into the circulation of cchfv in greece, 2000 ticks collected from livestock during 2012-2014 were tested. cchfv was detected in 36 of the 1290 (2.8%) tick pools (1-5 ticks per pool). two genetic lineages were identified: europe 1 and europ ...201728920177
seasonal distribution and faunistic of ticks in the alashtar county (lorestan province), iran.ticks are non-permanent obligate parasites that have considerable medical-veterinary and zoonosis importance. in this regard a study designed to investigate the distribution and fauna of ticks in the alashtar county in iran from april and march 2014.201729187953
tick-borne bacteria and protozoa detected in ticks collected from domestic animals and wildlife in central and southern portugal.ticks are vectors of many human and animal pathogens. the aim of this study was to screen bacteria and protozoa from ticks infesting domestic animals and wildlife collected in central and southern portugal. a total of 593 ticks, comprising 465 (78.4%) adults, 122 (20.6%) nymphs, and six (1.0%) larvae, were collected from 283 hosts of 25 different species (4 domestic and 21 wild). overall, the analysis of dna extracts prepared from ticks collected from hosts of 11 different species in the distric ...201728958791
diversity and distribution of ticks from domestic ruminants in lebanon.ticks (acari: ixodidae) are ectoparasites infesting livestock in every geographic area in the world and they are vectors of several viral, bacterial, and protozoan pathogens to animals and humans worldwide. a deep knowledge of the geographical distribution of these arthropods would have a key role in the control of tick-borne diseases. few data are available about tick presence in domestic ruminants in lebanon. the study aimed at providing an analysis of tick presence and distribution in lebanon ...201728675252
temporal and spatial distribution and species diversity of hard ticks (acari: ixodidae) in the eastern region of caspian sea.ticks are important parasites because of their voracious blood-feeding activity, and being as vectors for various agents of diseases in both human and livestock. this study was conducted in a monthly schedule from october 2014 to december 2015, at 45 study sites in three regions bordering the caspian sea, according to the topography including hillside, plain and coastal areas in collecting ticks from the body of sheep. alfa and beta biodiversity indices were calculated and compared between the t ...201627519473
external morphological anomalies in ixodid ticks from thrace, turkey.of 18,667 ticks examined, 33 specimens from species identified as haemaphysalis parva, hyalomma marginatum, hy. scupense, rhipicephalus bursa and rh. turanicus were found to have external morphological anomalies. anomalous ha. parva, hy. scupence and rh. turanicus were reported in this study for the first time. general anomalies manifested as asymmetry and deformations of the idiosoma, whereas local anomalies occurred in legs, exoskeleton, spiracular, adanal, subanal and accessory plates, mouthp ...201526264155
the effect of excluding ungulates on the abundance of ixodid ticks on wild rabbit (oryctolagus cuniculus).tick abundance depends heavily on host density, so reducing access to the host should reduce tick populations in a determined area. in this study we compared the parasitic index (pi = average number of ticks per rabbit) of two wild rabbit populations separated 16 years ago by a fence keeping ungulates from moving freely. two areas were selected (closed and open) wherein 50 wild rabbits per area were sampled for ticks. the pi in the open area (pi = 989.62) was significantly higher than in the clo ...201728840404
groel typing and phylogeny of anaplasma species in ticks from domestic and wild vertebrates.anaplasma species are globally distributed tick-borne bacteria causing a range of clinical conditions in domestic animals, wildlife, and human. nevertheless, data on presence and distribution of anaplasma strains in ticks are still lacking, especially in the mediterranean region. this study reports the molecular identification, genetic characterization and phylogeny of anaplasma strains of both veterinary and zoonotic importance in ticks collected from domestic and wild hosts sampled in a typica ...201829089249
tick-host conflict: immunoglobulin e antibodies to tick proteins in patients with anaphylaxis to tick bite.tick-borne infectious diseases and allergies are a growing problem worldwide. tick bite allergy has been associated with the direct effect of immunoglobulin e (ige) response to tick salivary antigens, or secondary to the induction of allergy to red meat consumption through ige antibodies against the carbohydrate α-gal (gal α 1-3gal β 1-(3)4glcnac-r). however, despite the growing burden of this pathology, the proteins associated with anaphylaxis to tick bite have not been characterized. to addres ...201728423486
genetic characterization of rhipicephalus sanguineus (sensu lato) ticks from dogs in portugal.the taxonomic status of the brown dog tick rhipicephalus sanguineus (sensu stricto) is a subject of on-going debate; there is a consensus that populations of this tick species should be referred to as r. sanguineus (sensu lato) until its taxonomic status is resolved. recent genetic studies revealed the existence of more than one lineage of r. sanguineus (s.l.) in temperate countries. in this study, we assessed the genetic identity of ticks collected from rural dogs living in several areas locate ...201728285602
comparative analysis of mitochondrial markers from four species of rhipicephalus (acari: ixodidae).mitochondrial sequences of four mitochondrial markers: 12s rrna, 16s rrna, cytochrome c oxidase subunit i (cox1) and cytochrome b (cytb) from four rhipicephalus species were analyzed to establish genetic relationships and enable molecular identification. field-collected samples from the species rhipicephalus annulatus, rhipicephalus bursa, rhipicephalus sanguineus and rhipicephalus turanicus were amplified by pcr and compared with genbank™ annotated sequences. pcr products were obtained using pr ...201324091196
comparative analyses of mitochondrial and nuclear genetic markers for the molecular identification of rhipicephalus spp.the genus rhipicephalus (acari: ixodidae) comprises a large number of vectors of pathogens of substantial medical and veterinary concern; however, species identification based solely on morphological features is often challenging. in the present study, genetic distance within selected rhipicephalus species (i.e., rhipicephalus bursa, rhipicephalus guilhoni, rhipicephalus muhsamae, rhipicephalus sanguineus sensu lato and rhipicephalus turanicus), were investigated based on molecular and phylogene ...201324103336
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