| dichloroacetate blocks aerobic glycolytic adaptation to attenuated measles virus and promotes viral replication leading to enhanced oncolysis in glioblastoma. | targeting reprogrammed energy metabolism such as aerobic glycolysis is a potential strategy for cancer treatment. however, tumors exhibiting low-rate glycolysis or metabolic heterogeneity might be resistant to such treatment. we hypothesized that a therapeutic modality that drove cancer cells to high-rate glycolysis might sensitize cancer cells to interference directed against metabolic flux. in this study, we found that attenuated oncolytic measles virus edmonston strain (mv-edm) caused gliobla ... | 0 | 25575816 |
| hepatitis a and e seroprevalence and associated risk factors: a community-based cross-sectional survey in rural amazonia. | hepatitis a virus (hav) and hepatitis e virus (hev) are both transmitted by the faecal-oral route, and represent common causes of acute hepatitis in developing countries. the endemicity of hav infection has shifted from high to moderate in brazil. human cases of hev infection seem to be rare, although the virus has been detected in swine livestock and effluents of slaughterhouses. this study was to determine the epidemiology of hepatitis a and e in one of the largest agricultural settlements in ... | 2014 | 25149658 |
| a novel live-attenuated vaccine candidate for mayaro fever. | mayaro virus (mayv) is an emerging, mosquito-borne alphavirus that causes a dengue-like illness in many regions of south america, and which has the potential to urbanize. because no specific treatment or vaccine is available for mayv infection, we capitalized on an ires-based approach to develop a live-attenuated mayv vaccine candidate. testing in infant, immunocompetent as well as interferon receptor-deficient mice demonstrated a high degree of attenuation, strong induction of neutralizing anti ... | 2014 | 25101995 |
| arthritogenic alphaviruses--an overview. | mosquito-transmitted alphaviruses causing human rheumatic disease are globally distributed and include chikungunya virus, ross river virus, barmah forest virus, sindbis virus, o'nyong-nyong virus and mayaro virus. these viruses cause endemic disease and, occasionally, large epidemics; for instance, the 2004-2011 chikungunya epidemic resulted in 1.4-6.5 million cases, with imported cases reported in nearly 40 countries. the disease is usually self-limiting and characterized by acute and chronic s ... | 2012 | 22565316 |
| peptide motif analysis predicts alphaviruses as triggers for rheumatoid arthritis. | rheumatoid arthritis (ra) develops in response to both genetic and environmental factors. the strongest genetic determinant is hla-dr, where polymorphisms within the p4 and p6 binding pockets confer elevated risk. however, low disease concordance across monozygotic twin pairs underscores the importance of an environmental factor, probably infectious. the goal of this investigation was to predict the microorganism most likely to interact with hla-dr to trigger ra under the molecular mimicry hypot ... | 2015 | 26476978 |
| emerging alphaviruses in the americas: chikungunya and mayaro. | chikungunya virus (chikv) and mayaro virus (mayv) are emergent arthropod-borne viruses that produce outbreaks of acute febrile illness with arthropathy. despite their different continental origins, chikv and mayv are closely related and are components of the semliki forest complex of the alphavirus (togaviridae). mayv and, more recently, chikv, which are both transmitted by aedes mosquitoes, have resulted in severe public health problems in the americas, including brazil. in this review, we pres ... | 2015 | 25626645 |
| detection of mayaro virus infections during a dengue outbreak in mato grosso, brazil. | arboviruses are common agents of human febrile illness worldwide. in dengue-endemic areas illness due to other arboviruses have been misdiagnosed as dengue based only on clinical-epidemiological data. in this study we investigated the presence of brazilian arboviruses in sera of 200 patients presenting acute febrile illness, during a dengue outbreak in sinop, mt, brazil. the results showed that 38 samples were positive to dengue virus (denv) type 1, two samples to denv type 4, and six to mayaro ... | 2015 | 25817238 |
| mayaro fever in an hiv-infected patient suspected of having chikungunya fever. | arboviruses impose a serious threat to public health services. we report a case of a patient returning from a work trip to the amazon basin with myalgia, arthralgia, fever, and headache. during this travel, the patient visited riverside communities. both dengue and chikungunya fevers were first suspected, tested for, and excluded. mayaro fever was then confirmed by reverse transcription polymerase chain reaction followed by next-generation sequencing and phylogenetic reconstruction. the increase ... | 2016 | 27812665 |
| thieno[2,3-b]pyridine derivatives: a new class of antiviral drugs against mayaro virus. | mayaro virus (mayv) is an arthropod-borne virus and a member of the family togaviridae, genus alphavirus. its infection leads to an acute illness accompanied by long-lasting arthralgia. to date, there are no antiviral drugs or vaccines against infection with mayv and resources for the prevention or treatment of other alphaviruses are very limited. mayv has served as a model to study the antiviral potential of several substances on alphavirus replication. in this work we evaluated the antiviral e ... | 2017 | 28213871 |
| mayaro virus: a forest virus primed for a trip to the city? | mayaro virus (mayv) is an emerging arthropod-borne virus (arbovirus). infection by mayv can produce mayaro virus disease (mayvd) which is usually a clinically diagnosed, acute, febrile illness associated with prolonged and painful joint inflammation and swelling. mayvd may be clinically indistinguishable from dengue, chikungunya fever, malaria, rabies, measles or other arboviral diseases. the full spectrum of disease, sequelae, routes of infection, virus shedding and any rarer means of transmiss ... | 2016 | 27989728 |
| macrophages as target cells for mayaro virus infection: involvement of reactive oxygen species in the inflammatory response during virus replication. | alphaviruses among the viruses that cause arthritis, consisting in a public health problem worldwide by causing localized outbreaks, as well as large epidemics in humans. interestingly, while the old world alphaviruses are arthritogenic, the new world alphaviruses cause encephalitis. one exception is mayaro virus (mayv), which circulates exclusively in south america but causes arthralgia and is phylogenetically related to the old world alphaviruses. although mayv-induced arthritis in humans is w ... | 2016 | 27627069 |
| a case of mayaro virus infection imported from french guiana. | emergence of arboviruses is a rising problem in several areas in the world. here we report a case of mayaro virus infection that was diagnosed in a french citizen presenting a dengue-like syndrome with prolonged arthralgia following a travel in french guiana. diagnosis was based on serological testing, a newly developed specific rt-pcr and sequencing. the real incidence of this viral infection among travelers is poorly known but this case is the first reported in a european area where aedes albo ... | 2016 | 26921736 |
| characterization of mitochondrial genome of haemagogus janthinomys (diptera: culicidae). | haemagogus janthinomys is a mosquito of high importance in public health due its involvement on natural wild cycles of two important arboviruses in the brazilian amazon region: yellow fever virus (flaviviridae, flavivirus) and mayaro virus (togaviridae, alphavirus). here, we have sequenced and described all the mitochondrial genes for the hg. janthinomys species. the complete coding sequence is14 937 bp long and includes 37 functional genes, of which 13 codes for proteins, 22 for trna and 2 for ... | 2017 | 26709451 |
| neutralising antibodies for mayaro virus in pantanal, brazil. | the pantanal hosts diverse wildlife species and therefore is a hotspot for arbovirus studies in south america. a serosurvey for mayaro virus (mayv), eastern (eeev), western (weev) and venezuelan (veev) equine encephalitis viruses was conducted with 237 sheep, 87 free-ranging caimans and 748 equids, including 37 collected from a ranch where a neurologic disorder outbreak had been recently reported. sera were tested for specific viral antibodies using plaque-reduction neutralisation test. from a t ... | 2015 | 25650766 |
| nmr study of non-structural proteins--part i: (1)h, (13)c, (15)n backbone and side-chain resonance assignment of macro domain from mayaro virus (mayv). | macro domains are adp-ribose-binding modules present in all eukaryotic organisms, bacteria and archaea. they are also found in non-structural proteins of several positive strand rna viruses such as alphaviruses. here, we report the high yield expression and preliminary structural analysis through solution nmr spectroscopy of the macro domain from new world mayaro alphavirus. the recombinant protein was well-folded and in a monomeric state. an almost complete sequence-specific assignment of its ( ... | 2015 | 25217003 |
| persisting arthralgia due to mayaro virus infection in a traveler from brazil: is there a risk for attendants to the 2014 fifa world cup? | the 2014 fifa world cup and the 2016 olympic games will attract large groups of visitors to brazil. these visitors will be at risk for different arboviral infections, some of which not well known outside endemic areas. we report a case of a 52-year-old dutch woman who presented with persistent arthralgia due to a mayaro virus (mayv) infection which she contracted in the amazon basin in brazil. mayv is a mosquito-borne alphavirus which primarily circulates in humid tropical forests of south ameri ... | 2014 | 24856445 |
| inhibition of mayaro virus infection by bovine lactoferrin. | mayaro virus (mayv) is an arbovirus linked to several sporadic outbreaks of a highly debilitating febrile illness in many regions of south america. mayv is on the verge of urbanization from the amazon region and no effective antiviral intervention is available against human infections. our aim was to investigate whether bovine lactoferrin (blf), an iron-binding glycoprotein, could hinder mayv infection. we show that blf promotes a strong inhibition of virus infection with no cytotoxic effects. m ... | 2014 | 24606707 |
| detection of arboviruses of public health interest in free-living new world primates (sapajus spp.; alouatta caraya) captured in mato grosso do sul, brazil. | a sero-epidemiological survey was undertaken to detect the circulation of arboviruses in free-living non-human primates. | 2014 | 24474008 |
| [mayaro: a re-emerging arbovirus in venezuela and latin america]. | mayaro virus produces nonspecific, sublethal disease symptoms, often confused with dengue, but with symptoms of arthalgias that can cause incapacitating disability. outbreaks have been localized and sporadic in the pan-amazonia forest since its first isolation in 1954 (trinidad and tobago). the literature available is scarce, diverse and dispersed. mayaro virus is an alphavirus, phylogenetically related to the semliki forest antigenic complex. una and mayaro viruses are the only viruses of this ... | 2012 | 23242303 |
| assessment of plaque assay methods for alphaviruses. | viruses from the alphavirus genus are responsible for numerous arboviral diseases impacting human health throughout the world. confirmation of acute alphavirus infection is based on viral isolation, identification of viral rna, or a fourfold or greater increase in antibody titers between acute and convalescent samples. in convalescence, the specificity of antibodies to an alphavirus may be confirmed by plaque reduction neutralization test. to identify the best method for alphavirus and neutraliz ... | 2013 | 23085307 |
| will mayaro virus be responsible for the next outbreak of an arthropod-borne virus in brazil? | mayaro virus (mayv) is an alphavirus from the togaviridae family and is transmitted mainly by hemagogus mosquitoes. this virus circulates in high-density tropical forests or rural areas of central and south america causing a disease characterized by high-grade fever, maculopapular skin rash and marked arthralgia that, in some patients, can persist for long periods after infection and may be misinterpreted as chikungunya. although only a few outbreaks involving this virus have been reported, in t ... | 2017 | 28688628 |
| multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of mayaro, oropouche, and oropouche-like viruses. | we describe a sensitive method for simultaneous detection of oropouche and oropouche-like viruses carrying the oropouche s segment, as well as the mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (rt-qpcr). a chimeric plasmid containing both mayaro and oropouche targets was designed and evaluated for the in vitro production of transcribed rna, which could be easily used as a non-infectious external control. to track false-negative results due t ... | 2017 | 28591313 |
| high frequency of mayaro virus igm among febrile patients, central brazil. | mayaro virus (mayv), an aedes mosquito-borne alphavirus, is endemic to brazil and other south america countries. we investigated dengue- and chikungunya-negative febrile patients visiting rural areas near goiânia, goiás, and found a high proportion (55%) of mayv igm. our findings suggest the presence of highly endemic foci of mayv in central brazil. | 2017 | 28518022 |
| emergence of recombinant mayaro virus strains from the amazon basin. | mayaro virus (mayv), causative agent of mayaro fever, is an arbovirus transmitted by haemagogus mosquitoes. despite recent attention due to the identification of several cases in south and central america and the caribbean, limited information on mayv evolution and epidemiology exists and represents a barrier to prevention of further spread. we present a thorough spatiotemporal evolutionary study of mayv full-genome sequences collected over the last sixty years within south america and haiti, re ... | 2017 | 28821712 |
| stabilization of a broadly neutralizing anti-chikungunya virus single domain antibody. | a single domain antibody (clone cc3) previously found to neutralize a vaccine strain of the chikungunya virus (prnt50 = 2. 5 ng/ml) was found to be broadly neutralizing. clone cc3 is not only able to neutralize a wild-type (wt) strain of chikungunya virus (chikv), but also neutralizes wt strains of mayaro virus (mayv) and ross river virus (rrv); both arthralgic, old world alphaviruses. interestingly, cc3 also demonstrated a degree of neutralizing activity against the new world alphavirus, venezu ... | 2021 | 33585527 |
| oxidative stress in mayaro virus infection. | mayaro virus (mayv) is a neglected tropical arbovirus that causes a febrile syndrome that is sometimes accompanied by incapacitating arthritis/arthralgia. the pathogenesis of mayv has not been completely defined and oxidative stress mediated by an increase in reactive oxygen species (ros) and/or depletion of antioxidant defences has been found to contribute to several aspects of viral disease. to investigate whether mayv induced oxidative stress in host cells, we monitored ros production, oxidat ... | 2017 | 28455127 |
| on the entry of an emerging arbovirus into host cells: mayaro virus takes the highway to the cytoplasm through fusion with early endosomes and caveolae-derived vesicles. | mayaro virus (mayv) is an emergent sylvatic alphavirus in south america, related to sporadic outbreaks of a chikungunya-like human febrile illness accompanied by severe arthralgia. despite its high potential for urban emergence, mayv is still an obscure virus with scarce information about its infection cycle, including the corresponding early events. even for prototypical alphaviruses, the cell entry mechanism still has some rough edges to trim: although clathrin-mediated endocytosis is quoted a ... | 2017 | 28462045 |