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biochemical and physiological changes during fruit development and ripening of two sweet cherry varieties with different levels of cracking tolerance.the aim of this study was to investigate the biochemical and metabolic changes, related to oxidative stress, ethylene and respiration, cell wall modification and primary metabolism, between a high ('prime giant') and a low ('cristalina') cracking susceptible sweet cherry cultivar during growth and ripening. while cherries are referred as a non-climacteric fruit, our results show that an increase of endogenous ethylene production at earlier fruit developmental stages is parallel to colour develop ...201727951491
a collection of european sweet cherry phenology data for assessing climate change.professional and scientific networks built around the production of sweet cherry (prunus avium l.) led to the collection of phenology data for a wide range of cultivars grown in experimental sites characterized by highly contrasted climatic conditions. we present a dataset of flowering and maturity dates, recorded each year for one tree when available, or the average of several trees for each cultivar, over a period of 37 years (1978-2015). such a dataset is extremely valuable for characterizing ...201627922629
the sweet cherry (prunus avium) flowering locus t gene is expressed during floral bud determination and can promote flowering in a winter-annual arabidopsis accession.ft gene is expressed in leaves and buds and is involved in floral meristem determination and bud development in sweet cherry. in woody fruit perennial trees, floral determination, dormancy and bloom, depends on perception of different environmental and endogenous cues which converge to a systemic signaling gene known as flowering locus t (ft). in long-day flowering plants, ft is expressed in the leaves on long days. the protein travels through the phloem to the shoot apical meristem, where it in ...201627878597
high throughput sequencing reveals a novel fabavirus infecting sweet cherry.the genus fabavirus currently consists of five species represented by viruses that infect a wide range of hosts but none reported from temperate climate fruit trees. a virus with genomic features resembling fabaviruses (tentatively named prunus virus f, prvf) was revealed by high throughput sequencing of extracts from a sweet cherry tree (prunus avium). prvf was subsequently shown to be graft transmissible and further identified in three other non-symptomatic prunus spp. from different geographi ...201727815695
evaluation of physicochemical characteristics and antioxidant property of prunus avium gum exudates.in this study some physicochemical properties and elemental analysis of prunus avium gum exudates were investigated. the gum studied had, on average, 75.14% carbohydrate, 11.3% uronic acids, 1.11% protein, 7.53% moisture content (w.b.) and 3.12% ash. measured values for the angle of repose, carr's index and hausner ratio showed the good flow ability for the gum powder. the viscosity of 1% aqueous solution of the gum exhibited a newtonian type of flow and with ph reduction the swelling index was ...201627593240
characterization of autochthonous sweet cherry cultivars (prunus avium l.) of southern italy for fruit quality, bioactive compounds and antioxidant activity.characterizing germplasm collections of autochthonous cultivars for fruit quality traits could be a successful approach for selection, improvement of organoleptic quality and levels of antioxidants of crop produce, and development of new market opportunities and coherent strategies for conservation and valorization. the aim of the study was the evaluation of fruit physicochemical traits as well as the content of bioactive compounds and the antioxidant capacity in 25 sweet cherry autochthonous cu ...201727766652
linking hormonal profiles with variations in sugar and anthocyanin contents during the natural development and ripening of sweet cherries.sweet cherries are highly appreciated by consumers worldwide and are usually cold-stored during postharvest to prevent over-ripening before distribution to the market. sweet cherry is a non-climacteric fruit, for which ripening is known to be regulated by abscisic acid. here we aimed to examine the hormone profiles, including measurements of abscisic acid, auxins, cytokinins and gibberellins by ultrahigh performance liquid chromatography coupled to tandem mass spectrometry (uhplc-ms/ms), in rela ...201627475901
ultrasound treatment: effect on physicochemical, microbial and antioxidant properties of cherry (prunus avium).the cherry was treated with ultrasonic waves (33 khz, 60 w) at different time intervals (10, 20, 30, 40, 60 min) and study was carried out to analyze the change in physico-chemical properties (tss, ph, color, acidity and firmness), antioxidant potential and microbial load of the fruit during the storage period of 15 days at 4 °c. it was observed that ultrasound treatment (us) between 30 and 40 min showed better retention of color of the fruit during the storage period. the antioxidant assays (dp ...201627478231
genetic diversity of some iranian sweet cherry (prunus avium) cultivars using microsatellite markers and morphological traits.the aim of this study was to characterize 23 important iranian sweet cherry (prunus avium) cultivars collected from different provinces of iran and 1 foreign cultivar, which was used as control, considered for breeding programs by using 21 microsatellite markers and 27 morphological traits. in sweet cherry (prunus avium) accessions, leaf, fruit, and stone morphological characters were evaluated during two consecutive years. the study revealed a high variability in the set of evaluated sweet cher ...201627266181
dietary sweet cherry anthocyanins attenuates diet-induced hepatic steatosis by improving hepatic lipid metabolism in mice.anthocyanins have been reported to have beneficial effects on obesity and obesity-related metabolic disorders (e.g., insulin resistance and dyslipidemia). the objective of this study was to examine the beneficial effects of sweet cherry anthocyanins (swcn) on high-fat diet-induced liver steatosis and investigate the underlying molecular mechanism.201727158052
proteomic comparison of fruit ripening between 'hedelfinger' sweet cherry (prunus avium l.) and its somaclonal variant 'hs'.the somaclonal variant hs, from sweet cherry (prunus avium l.) 'hedelfinger' (h), was previously selected for reduced tree vegetative vigor and lesser canopy density. in this work, we compared h and hs fruits at early unripe (green) and full ripe (dark red) stages by biochemical and proteomic approaches. the main biochemical parameters showed that fruit quality was not affected by somaclonal variation. the proteomic analysis identified 39 proteins differentially accumulated between h and hs frui ...201627144542
recognition of a wide-range of s-rnases by s locus f-box like 2, a general-inhibitor candidate in the prunus-specific s-rnase-based self-incompatibility system.many species in the rosaceae, the solanaceae, and the plantaginaceae exhibit s-rnase-based gametophytic self-incompatibility (gsi). this system comprises s-ribonucleases (s-rnases) as the pistil s determinant and a single or multiple f-box proteins as the pollen s determinants. in prunus, pollen specificity is determined by a single s haplotype-specific f-box protein (sfb). the results of several studies suggested that sfb exerts cognate s-rnase cytotoxicity, and a hypothetical general inhibitor ...201627071402
turn-off fluorescence sensor for the detection of ferric ion in water using green synthesized n-doped carbon dots and its bio-imaging.this paper reports turn-off fluorescence sensor for fe(3+) ion in water using fluorescent n-doped carbon dots as a probe. a simple and efficient hydrothermal carbonization of prunus avium fruit extract for the synthesis of fluorescent nitrogen-doped carbon dots (n-cds) is described. this green approach proceeds quickly and provides good quality n-cds. the mean size of synthesized n-cds was approximately 7nm calculated from the high-resolution transmission electron microscopic images. x-ray photo ...201626994332
absolute quantification of pru av 2 in sweet cherry fruit by liquid chromatography/tandem mass spectrometry with the use of a stable isotope-labelled peptide.pru av 2, a pathogenesis-related (pr) protein present in the sweet cherry (prunus avium l.) fruit, is the principal allergen of cherry and one of the chief causes of pollen food syndrome (oral allergy syndrome). in this study, a quantitative assay for this protein was developed with the use of the protein absolute quantification (aqua) method, which consists of liquid chromatography/tandem mass spectrometry (lc/ms/ms) employing tgc[cam]stdasgk[(13)c6,(15)n2], a stable isotope-labelled internal s ...201626988485
[effects of exogenous glucose and starch on soil carbon metabolism of root zone and root function in potted sweet cherry].one-year-old potted sweet cheery trees were treated with 4 g · kg(-1) exogenous glucose or starch and with non-addition of exogenous carbon as the control for up to 60 days. soil of root zone was sampled to analyze soil microbial biomass carbon, activities of invertase and amylase and microbial community functional diversity during the 60-day treatment, and roots were sampled for analysis of root respiratory rate, respiratory pathways and root viability after treatment for 30 days. results showe ...201526915183
genetic diversity, linkage disequilibrium, population structure and construction of a core collection of prunus avium l. landraces and bred cultivars.depiction of the genetic diversity, linkage disequilibrium (ld) and population structure is essential for the efficient organization and exploitation of genetic resources. the objectives of this study were to (i) to evaluate the genetic diversity and to detect the patterns of ld, (ii) to estimate the levels of population structure and (iii) to identify a 'core collection' suitable for association genetic studies in sweet cherry.201626912051
changes in fine-root production, phenology and spatial distribution in response to n application in irrigated sweet cherry trees.factors regulating fine-root growth are poorly understood, particularly in fruit tree species. in this context, the effects of n addition on the temporal and spatial distribution of fine-root growth and on the fine-root turnover were assessed in irrigated sweet cherry trees. the influence of other exogenous and endogenous factors was also examined. the rhizotron technique was used to measure the length-based fine-root growth in trees fertilized at two n rates (0 and 60 kg ha(-1)), and the above- ...201626888890
complete nucleotide sequence of little cherry virus 1 (lchv-1) infecting sweet cherry in china.little cherry virus 1 (lchv-1), associated with little cherry disease (lcd), has a significant impact on fruit quality of infected sweet cherry trees. we report the full genome sequence of an isolate of lchv-1 from taian, china (lchv-1-ta), detected by small-rna deep sequencing and amplified by overlapping rt-pcr. the lchv-1-ta genome was 16,932 nt in length and contained nine open reading frames (orfs), with sequence identity at the overall genome level of 76%, 76%, and 78% to lchv-1 isolates y ...201626733294
a single-step solid phase extraction for the simultaneous determination of 8 mycotoxins in fruits by ultra-high performance liquid chromatography tandem mass spectrometry.a simple and rapid extraction procedure for the simultaneous determination of eight mycotoxins (alternaria toxins, ochratoxin a, patulin, citrinin) in a variety of fruit matrices has been developed using ultra high performance liquid chromatography coupled to tandem mass spectrometry. the procedure involves a one-step cleanup using homemade solid phase extraction (spe) cartridges. by comparative evaluation among six various adsorbents (c18, psa, hlb, mcx, silica, nh2), the combination of mcx and ...201626726936
phenolic compounds, volatiles, and sensory characteristics of twelve sweet cherry (prunus avium l.) cultivars grown in turkey.the paper reports the phenolic, anthocyanin, and volatile compounds and sensory characteristics of 12 cultivars of sweet cherries including cvs. belge, bing, dalbasti, durona di cesena, lambert, merton late, starks gold, summit, sweetheart, van, vista, and 0-900 ziraat. eight individual phenolic compounds were determined by the hplc-dad method. among these cherries, cvs. bing, durona di cesena, and lambert contained higher levels of total individual phenolic compounds than the other cultivars. s ...201626646816
(1)h nmr metabolic fingerprinting to probe temporal postharvest changes on qualitative attributes and phytochemical profile of sweet cherry fruit.sweet cherry fruits (prunus avium cvs. 'canada giant', 'ferrovia') were harvested at commercial maturity stage and analyzed at harvest and after maintenance at room temperature (storage at ∼20°c, shelf life) for 1, 2, 4, 6, and 8 days, respectively. fruit were initially analyzed for respiration rate, qualitative attributes and textural properties: 'canada giant' fruit were characterized by higher weight losses and stem browning index, being more intense over the late stages of shelf life period; ...201526617616
mapping of candidate genes involved in bud dormancy and flowering time in sweet cherry (prunus avium).the timing of flowering in perennial plants is crucial for their survival in temperate climates and is regulated by the duration of bud dormancy. bud dormancy release and bud break depend on the perception of cumulative chilling during endodormancy and heat during the bud development. the objectives of this work were to identify candidate genes involved in dormancy and flowering processes in sweet cherry, their mapping in two mapping progenies 'regina' × 'garnet' and 'regina' × 'lapins', and to ...201526587668
paternal-specific s-allele transmission in sweet cherry (prunus avium l.): the potential for sexual selection.homomorphic self-incompatibility is a well-studied example of a physiological process that is thought to increase population diversity and reduce the expression of inbreeding depression. whereas theoretical models predict the presence of a large number of s-haplotypes with equal frequencies at equilibrium, unequal allele frequencies have been repeatedly reported and attributed to sampling effects, population structure, demographic perturbation, sheltered deleterious mutations or selection pressu ...201626559165
[determination of anthocyanins in the peel of sweet cherry by ultra performance liquid chromatography tandem mass spectrometry].a method for the determination of seven anthocyanins in the peel of sweet cherry was developed by ultra performance liquid chromatography-tandem mass spectrometry (uplc-ms/ms). the sample was extracted by methanol containing 0.1% (v/v) hydrochloric acid, and then purified by ab-8 macroporous resins. the separation was carried out on a phenomenex kinetex column (100 mm x 4.6 mm, 2.6 rm) with mobile phase of 0.1% (v/v) formic acid aqueous solution containing 5 mmol/l ammonium formate and methanol. ...201526536760
construction of commercial sweet cherry linkage maps and qtl analysis for trunk diameter.a cross between the sweet cherry (prunus avium) cultivars 'wanhongzhu' and 'lapins' was performed to create a mapping population suitable for the construction of a linkage map. the specific-locus amplified fragment (slaf) sequencing technique used as a single nucleotide polymorphism (snp) discovery platform and generated 701 informative genotypic assays; these, along with 16 microsatellites (ssrs) and the incompatibility (s) gene, were used to build a map which comprised 8 linkage groups (lgs) a ...201526516760
a study of operator performance for a mechanical sweet cherry harvester: comparison between manual and remote-controlled operation.an experimental mechanical sweet cherry harvester was upgraded by changing its rapid impact mechanism to a continuous impact mechanism, in which an end effector (impactor) is positioned on fruit-loaded branches to transfer vibrational energy for fruit removal, and by adding remote control capability. before the upgrade, the operator was seated at the rear of the harvester to manually control its operation. adding remote control capability allowed operator mobility while operating the machine. th ...201526373213
prunetin signals via g-protein-coupled receptor, gpr30(gper1): stimulation of adenylyl cyclase and camp-mediated activation of mapk signaling induces runx2 expression in osteoblasts to promote bone regeneration.prunetin is found in red clover and fruit of prunus avium (red cherry). the effect of prunetin on osteoblast function, its mode of action and bone regeneration in vivo were investigated. cultures of primary osteoblasts, osteoblastic cell line and hek293t cells were used for various in vitro studies. adult female rats received drill-hole injury at the femur diaphysis to assess the bone regenerative effect of prunetin. prunetin at 10nm significantly (a) increased proliferation and differentiation ...201526345541
mining microsatellites in the peach genome: development of new long-core ssr markers for genetic analyses in five prunus species.a wide inventory of molecular markers is nowadays available for individual fingerprinting. microsatellites, or simple sequence repeats (ssrs), play a relevant role due to their relatively ease of use, their abundance in the plant genomes, and their co-dominant nature, together with the availability of primer sequences in many important agricultural crops. microsatellites with long-core motifs are more easily scored and were adopted long ago in human genetics but they were developed only in few c ...201526185739
prunus transcription factors: breeding perspectives.many plant processes depend on differential gene expression, which is generally controlled by complex proteins called transcription factors (tfs). in peach, 1533 tfs have been identified, accounting for about 5.5% of the 27,852 protein-coding genes. these tfs are the reference for the rest of the prunus species. tf studies in prunus have been performed on the gene expression analysis of different agronomic traits, including control of the flowering process, fruit quality, and biotic and abiotic ...201526124770
spring frost vulnerability of sweet cherries under controlled conditions.spring frost is a significant production hazard in nearly all temperate fruit-growing regions. sweet cherries are among the first fruit varieties starting their development in spring and therefore highly susceptible to late frost. temperatures at which injuries are likely to occur are widely published, but their origin and determination methods are not well documented. in this study, a standardized method was used to investigate critical frost temperatures for the sweet cherry cultivar 'summit' ...201626022603
construction of high density sweet cherry (prunus avium l.) linkage maps using microsatellite markers and snps detected by genotyping-by-sequencing (gbs).linkage maps are valuable tools in genetic and genomic studies. for sweet cherry, linkage maps have been constructed using mainly microsatellite markers (ssrs) and, recently, using single nucleotide polymorphism markers (snps) from a cherry 6k snp array. genotyping-by-sequencing (gbs), a new methodology based on high-throughput sequencing, holds great promise for identification of high number of snps and construction of high density linkage maps. in this study, gbs was used to identify snps from ...201526011256
expression and anthocyanin biosynthesis-modulating potential of sweet cherry (prunus avium l.) myb10 and bhlh genes.anthocyanins are essential contributors to fruit coloration, an important quality feature and a breed determining trait of a sweet cherry fruit. it is well established that the biosynthesis of anthocyanins is regulated by an interplay of specific transcription factors belonging to myb and bhlh families accompanied by a wd40 protein. in this study, we isolated and analyzed pawd40, pabhlh3, pabhlh33, and several closely related myb10 gene variants from different cultivars of sweet cherry, analyzed ...201525978735
breaking seed dormancy of three orthodox mediterranean rosaceae species.biodiversity levels could be enhanced when regenerating a site by seed-derived seedlings. however, seed dormancy poses limitations for many species. as a result, nurseries either produce seedlings from species where dormancy is not an obstacle, or they propagate through cuttings with the risk of decreasing the genetic diversity within and among species at the regenerated sites. in the present study, breaking of seed dormancy was investigated in valuable mediterranean species of prunus avium, pru ...201525895254
effect of pulsed electric fields on the flavour profile of red-fleshed sweet cherries (prunus avium var. stella).the aim of this research was to study the effect of pulsed electric fields (pef) on the flavour profile of red-fleshed sweet cherries (prunus avium variety stella). the cherry samples were treated at a constant pulse frequency of 100 hz, a constant pulse width of 20 μs, different electric field strengths between 0.3 and 2.5 kv/cm and specific energy ranging from 31 to 55 kj/kg. volatile compounds of samples were analysed using an automated headspace solid phase microextraction (hs-spme) method c ...201525806548
comparative transcriptome analysis of genes involved in anthocyanin biosynthesis in the red and yellow fruits of sweet cherry (prunus avium l.).fruit color is one of the most important economic traits of the sweet cherry (prunus avium l.). the red coloration of sweet cherry fruit is mainly attributed to anthocyanins. however, limited information is available regarding the molecular mechanisms underlying anthocyanin biosynthesis and its regulation in sweet cherry.201525799516
physiological and biochemical changes relating to postharvest splitting of sweet cherries affected by calcium application in hydrocooling water.hydrocooling sweet cherries shortly after harvest (4h) and then transporting fruit in cold flume water during packing are used to maximize postharvest quality, but can cause fruit splitting. this study demonstrated that cherry fruit (two splitting-susceptible cultivars) absorbed ca in a quadratic polynomial manner with increasing cacl2 concentration from 0.2% to 2.0% in cold water (0°c) for 5 min, but did not take up cl. the enhanced tissue ca content reduced splitting potential by decreasing fr ...201525794746
physicochemical characteristics, antioxidant activity, organic acid and sugar contents of 12 sweet cherry (prunus avium l.) cultivars grown in turkey.physical characteristics, antioxidant activity and chemical constituents of 12 cultivars (prunus avium l.) of sweet cherry (belge, bing, dalbasti, durona di cesena, lambert, merton late, starks gold, summit, sweetheart, van, vista, and 0-900 ziraat) were investigated. significant differences (p < 0.05) were observed among tested cultivars for ph, total soluble solid, hardness, color parameters, antioxidant activities and pomological measurements (p < 0.05). the color parameters were important to ...201525631389
characterization of four popular sweet cherry cultivars grown in greece by volatile compound and physicochemical data analysis and sensory evaluation.volatile compounds, physicochemical and sensory attributes of four sweet cherry cultivars (canada giant, ferrovia, lapins and skeena) grown in northern greece were determined. eighteen volatile compounds were identified and semi-quantified in cherries using solid phase micro extraction in combination with gas chromatography/mass spectrometry (spme-gc/ms). carbonyl compounds were the most abundant in sweet cherry aroma, followed by alcohols, esters and hydrocarbons/terpenes. cherry cultivars in o ...201525629454
effect of β-aminobutyric acid on cell wall modification and senescence in sweet cherry during storage at 20°c.the effects of postharvest β-aminobutyric acid (baba) treatment on fruit firmness, pectin degrading enzymes, cell wall constituents and microstructural alterations of pericarp in sweet cherry fruit were investigated. baba significantly delayed the decline of fruit firmness and inhibited the increase of membrane permeability and the accumulation of malondialdehyde in cherries. the baba-treated fruit exhibited significantly higher contents of water-soluble pectin, cdta-soluble pectin, na2co3-solub ...201525577108
bioassay-based isolation and identification of phenolics from sweet cherry that promote active glucose consumption by hepg2 cells.a variety of phenolics had been found to be functional in promoting cellular glucose consumption that is important for blood glucose regulation. sweet cherry (prunus avium) is rich in such kinds of phenolics, including hydrocinnamic acids, anthocyanins, flavonols, and flavan-3-ols. furthermore, a sweet cherry phenolics-rich extract (pre) was found to be effective in promoting hepg2 glucose consumption. seventeen components were preliminarily identified by hplc-esi-ms, including 9 hydrocinnamic a ...201525559482
chemical characterisation and bioactive properties of prunus avium l.: the widely studied fruits and the unexplored stems.the aim of this study was to characterise sweet cherry regarding nutritional composition of the fruits, and individual phytochemicals and bioactive properties of fruits and stems. the chromatographic profiles in sugars, organic acids, fatty acids, tocopherols and phenolic compounds were established. all the preparations (extracts, infusions and decoctions) obtained using stems revealed higher antioxidant potential than the fruits extract, which is certainly related with its higher phenolic compo ...201525466123
high concentrations of anthocyanins in genuine cherry-juice of old local austrian prunus avium varieties.antioxidant activity and polyphenols were quantified in vapour-extracted juice of nine austrian, partially endemic varieties of sweet cherry (prunus avium): cv. 'spätbraune von purbach', cv. 'early rivers', cv. 'joiser einsiedekirsche', cv. 'große schwarze knorpelkirsche' and four unidentified local varieties. additionally the effect of storage was evaluated for six of the varieties. a variety showing the highest antioxidant capacity (9.64 μmol trolox equivalents per ml), total polyphenols (2747 ...201525466109
physicochemical characterisation of four cherry species (prunus spp.) grown in china.the physicochemical characteristics of four cherry species (prunus avium, prunus cerasus, prunus pseudocerasus and prunus tomentosa) were evaluated. inter-species variability was greater than intra-species differences. glucose and fructose were the main sugars, and malic acid was the main organic acid in all species. combining hplc-dad and lc-esi-ms/ms technologies, total 25 phenolic components were preliminarily identified. p. avium was characterised by high fruit weight, edible proportion, sug ...201525466099
compositional changes in cell wall polysaccharides from five sweet cherry (prunus avium l.) cultivars during on-tree ripening.excessive softening is a major cause of postharvest deterioration during transportation and storage of fresh cherries. in continuing our studies to identify the factors determining the textural differences between sweet cherry fruit genotypes, we evaluated the solubilization, depolymerization, and monosaccharide composition of pectin and hemicelluloses from five sweet cherry cultivars ('chelan', 'sumele', 'brooks', 'sunburst', and 'regina') with contrasting firmness and cracking susceptibility a ...201425434844
molecular characterization and expression analyses of an anthocyanin synthase gene from magnolia sprengeri pamp.anthocyanin synthase (ans), which catalyzes the conversion of colorless leucoanthocyanins into colored anthocyanins, is a key enzyme in the anthocyanin biosynthetic pathway. it plays important roles in plant development and defense. an ans gene designated as msans was cloned from magnolia sprengeri using rapid amplification of complementary dna (cdna) ends technology. the full-length msans is 1171-bp long and contains a 1080-bp open reading frame encoding a 360 amino acid polypeptide. in a seque ...201525315387
electronic nose and isotope ratio mass spectrometry in combination with chemometrics for the characterization of the geographical origin of italian sweet cherries.sweet cherries from two italian regions, apulia and emilia romagna, were analysed using electronic nose (en) and isotope ratio mass spectrometry (irms), with the aim of distinguishing them according to their geographic origin. the data were elaborated by statistical techniques, examining the en and irms datasets both separately and in combination. preliminary exploratory overviews were performed and then linear discriminant analyses (lda) were used for classification. regarding en, different app ...201525306321
in-time and in-space tandem mass spectrometry to determine the metabolic profiling of flavonoids in a typical sweet cherry (prunus avium l.) cultivar from southern italy.this paper presents a comprehensive analytical methodology, based on 'in-time' and 'in-space' tandem mass spectrometry (ms) techniques, to identify and quantify flavonoid compounds in a typical italian sweet cherry cultivar (cv. ferrovia). five anthocyanins, four flavan-3-ols and nine flavonols were determined by means of hyphenated high-performance liquid chromatography - multi-stage ms (hplc-ms(n)) analyses (ms(n) up to ms(4)), among which quercetin-3-o-rutinoside-7-o-glucoside, kaempferol-3-o ...201425303392
characterization of cuticle composition after cold storage of "celeste" and "somerset" sweet cherry fruit.cuticle composition and structure may be relevant factors affecting the storage potential of fruits, but very few studies have analyzed fruit cuticle composition from a postharvest perspective. in this work, the chemical composition of waxes and cutin (major cuticular components) was analyzed in cuticle samples isolated from "celeste" and "somerset" cherries (prunus avium l.) after cold storage at 0 °c. total cuticle amounts per surface unit (μg cm(-2)) increased along with cold storage. the tri ...201425089645
post-storage cell wall metabolism in two sweet cherry (prunus avium l.) cultivars displaying different postharvest performance.the biochemical processes underlying firmness loss of sweet cherry (prunus avium l.) fruit are poorly understood. studies on cell wall metabolism of sweet cherry have been generally undertaken during on-tree development or at harvest maturity, while published reports on postharvest changes are scarce and fragmentary. in this work, cell wall modifications after storage at 0 ℃ were studied in two cherry cultivars ('celeste' and 'somerset') displaying different postharvest potential. firmness was l ...201524986906
biaxial tensile tests identify epidermis and hypodermis as the main structural elements of sweet cherry skin.the skin of developing soft and fleshy fruit is subjected to considerable growth stress, and failure of the skin is associated with impaired barrier properties in water transport and pathogen defence. the objectives were to establish a standardized, biaxial tensile test of the skin of soft and fleshy fruit and to use it to characterize and quantify mechanical properties of the sweet cherry (prunus avium) fruit skin as a model. a segment of the exocarp (es) comprising cuticle, epidermis, hypoderm ...201424876301
quality and antioxidant properties on sweet cherries as affected by preharvest salicylic and acetylsalicylic acids treatments.the effects of salicylic acid (sa) or acetylsalicylic acid (asa) treatments during on-tree cherry growth and ripening on fruit quality attributes, especially those related with the content on bioactive compounds and antioxidant activity were analysed in this research. for this purpose, two sweet cherry cultivars, 'sweet heart' and 'sweet late', were used and sa or asa treatments, at 0.5, 1.0 and 2.0mm concentrations, were applied at three key points of fruit development (pit hardening, initial c ...201424799232
the effect of postharvest calcium application in hydro-cooling water on tissue calcium content, biochemical changes, and quality attributes of sweet cherry fruit.to improve storage/shipping quality of sweet cherry (prunus avium l.), the effect of calcium chloride (cacl2) added to hydro-cooling water on physiological and biochemical processes related to fruit and pedicel quality was investigated on two major cultivars. the fruit tissue ca content increased up to 29-85% logarithmically for 'sweetheart' and 39-188% linearly for 'lapins' as cacl2 rate increased from 0.2% to 2.0% at 0 °c for 5 min. the increase of fruit tissue ca content was accompanied by re ...201424799204
mitochondrial coi and morphological evidence for host specificity of the black cherry aphids myzus cerasi (fabricius, 1775) collected from different cherry tree species in europe (hemiptera, aphididae).partial sequences of the mitochondrial coi gene of forty eight european and two turkish population samples of myzus cerasi from different winter hosts (prunus spp.) were subjected to phylogenetic analyses. the analysed m. cerasi samples emerged as paraphyletic relative to a myzus borealis sample used as an out-group, and formed two major clades in neighbor joining, maximum parsimony, maximum likelihood and bayesian inference trees, corresponding to subspecies living specifically on prunus avium ...201424715766
preharvest application of oxalic acid increased fruit size, bioactive compounds, and antioxidant capacity in sweet cherry cultivars (prunus avium l.).trees of 'sweet heart' and 'sweet late' sweet cherry cultivars (prunus avium l.) were treated with oxalic acid (oa) at 0.5, 1.0, and 2.0 mm at 98, 112, and 126 days after full blossom. results showed that all treatments increased fruit size at harvest, manifested by higher fruit volume and weight in cherries from treated trees than from controls, the higher effect being found with 2.0 mm oa (18 and 30% higher weight for 'sweet heart' and 'sweet late', respectively). other quality parameters, suc ...201424684635
temperature-related development and population parameters for drosophila suzukii (diptera: drosophilidae) on cherry and blueberry.temperature-related studies were conducted on drosophila suzukii matsumura (diptera: drosophilidae: drosophilini). from 10-28°c, temperature had a significant impact on blueberries, vaccinium corymbosum l. (ericales: ericaceae), and cherries, prunus avium (l.) l. 1755 (rosales: rosaceae), important commercial hosts of d. suzukii. temperature had a significant influence on d. suzukii developmental period, survival, and fecundity, with decreasing developmental periods as temperatures increased to ...201424612968
different compounds are extracted with different time courses from fruits during microwave hydrodiffusion: examples and possible causes.we set out to determine how nutrients diffuse during extraction, using fractional collection. the highest concentrations of sugars (195.5, 64.8 and 60.8g/l, respectively for grape, 'najbolia' plum and apricot) were found for the earliest stages of extraction, with a decrease in concentration (to 41.4g/l, 48.2g/l and 1.7g/l, respectively) at the end of extraction process. total polyphenols showed the same trends for plum and apricot (from 4.1g/l to 2.9g/l for 'najbolia' plum, from 2.2 to 0.2g/l f ...201424518331
eating quality and health-promoting properties of two sweet cherry (prunus avium l.) cultivars stored in passive modified atmosphere.two sweet cherry cultivars ('new star' and 'sweet heart') were chosen to explore the impact of passive modified atmosphere packaging on the eating quality and health-promoting properties of fruit. packaged and unpackaged fruits were stored at 0 ℃ for 15 or 30 days, followed by zero or three days at 20 ℃, after which the analyses were undertaken. in most cases, modified atmosphere packaging helped preserving higher firmness values and reducing the incidence of alterations and decay in both cultiv ...201524474188
morphological characterization of cherry rootstock candidates selected from central and east black sea regions in turkey.the use of rootstocks particularly for sweet cherry cultivars is of great importance for successful and sustainable production. choosing the right cherry rootstocks is just as important as choosing the right cultivar. in this study, 110 sweet cherry, 30 sour cherry, and 41 mahaleb types displaying rootstock potential for sweet cherry cultivars were selected from central and east black sea regions in turkey. the morphologic characteristics of the studied genotypes were compared with the standard ...201324453921
free and glycosidically bound aroma compounds in cherry (prunus avium l.).this paper reports the occurrence of both free and glycosidically bound aroma compounds in three sweet cherry cultivars ('hongdeng', 'hongyan' and 'rainier'), with 97 compounds being identified in the three cultivars. the major free volatile compounds found were hexanal, (e)-2-hexenal, (e)-2-hexen-1-ol, benzyl alcohol and benzaldehyde. the major bound volatile compounds found were benzyl alcohol, geraniol, 2-phenylethanol. also 4-vinylphenol was found in cherry fruit for the first time, and has ...201424444903
genetic determinism of phenological traits highly affected by climate change in prunus avium: flowering date dissected into chilling and heat requirements.the present study investigated the genetic determinism of flowering date (fd), dissected into chilling (cr) and heat (hr) requirements. elucidation of the genetic determinism of flowering traits is crucial to anticipate the increasing of ecological misalignment of adaptative traits with novel climate conditions in most temperate-fruit species. cr and hr were evaluated over 3 yr and fd over 5 yr in an intraspecific sweet cherry (prunus avium) f1 progeny, and fd over 6 yr in a different f1 progeny ...201424417538
transcriptional dynamics of the developing sweet cherry (prunus avium l.) fruit: sequencing, annotation and expression profiling of exocarp-associated genes.the exocarp, or skin, of fleshy fruit is a specialized tissue that protects the fruit, attracts seed dispersing fruit eaters, and has large economical relevance for fruit quality. development of the exocarp involves regulated activities of many genes. this research analyzed global gene expression in the exocarp of developing sweet cherry (prunus avium l., 'regina'), a fruit crop species with little public genomic resources. a catalog of transcript models (contigs) representing expressed genes wa ...201426504533
breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective.this review is an overview of traditional and modern breeding methodologies being used to develop new prunus cultivars (stone fruits) with major emphasis on peach, sweet cherry and japanese plum. to this end, common breeding tools used to produce seedlings, including in vitro culture tools, are discussed. additionally, the mechanisms of inheritance of many important agronomical traits are described. recent advances in stone fruit transcriptomics and genomic resources are providing an understandi ...201324346068
molecular analysis of east anatolian traditional plum and cherry accessions using ssr markers.we conducted ssr analyses of 59 accessions, including 29 traditional plum (prunus domestica), 24 sweet cherry (prunus avium), and 1 sour cherry (prunus cerasus) selected from east anatolian gene sources and 3 plum and 2 cherry reference accessions for molecular characterization and investigation of genetic relationships. eight ssr loci [1 developed from the apricot (udap-404), 4 from the peach (udp96-010, udp96-001, udp96-019, pchgms1) and 3 from the cherry (ucd-ch13, ucd-ch17, ucd-ch31) genome] ...201324301792
effects of 10-ghz microwaves on hematological parameters in swiss albino mice and their modulation by prunus avium.the objective of this study was to investigate the modulatory role of prunus avium fruit extract (pae) on several blood parameters after exposure to 10-ghz microwaves. swiss albino mice from an inbred colony were selected and divided into 3 groups. mice in group i served as the control; they were placed in a plexiglas cage (without energizing the system) for 2 hours/day for 30 consecutive days. group ii mice were exposed to 10-ghz microwaves for 2 hours/day for 30 consecutive days. mice in group ...201324266407
inhibitory effects of sweet cherry anthocyanins on the obesity development in c57bl/6 mice.in the present study, purified sweet cherry anthocyanins (cacn) were evaluated to determine their inhibitory effects on adipocyte differentiation of 3t3-l1 cells and their anti-obesity properties in male c57bl/6 mice fed with high-fat diet (hfd). cacn prevented hfd-induced obesity in c57bl/6 mice. in vivo experiment revealed that 40 and 200 mg/kg of cacn in food reduced the body weight by 5.2% and 11.2%, respectively. cacn supplementation could also reduce the size of adipocytes, leptin secretio ...201424224922
down-regulation of photosynthesis following girdling, but contrasting effects on fruit set and retention, in two sweet cherry cultivars.sweet cherry (prunus avium) trees were manipulated to analyse the contribution of soluble sugars to sink feedback down-regulation of leaf net co2 assimilation rate (anet) and fruit set and quality attributes. total soluble sugar concentration and anet were measured in the morning on fully expanded leaves of girdled branches in two sweet cherry cultivars, 'kordia' and 'sylvia' characterised typically by low and high crop load, respectively. leaves on girdled trees had higher soluble sugar concent ...201324189522
cell number regulator genes in prunus provide candidate genes for the control of fruit size in sweet and sour cherry.striking increases in fruit size distinguish cultivated descendants from small-fruited wild progenitors for fleshy fruited species such as solanum lycopersicum (tomato) and prunus spp. (peach, cherry, plum, and apricot). the first fruit weight gene identified as a result of domestication and selection was the tomato fw2.2 gene. members of the fw2.2 gene family in corn (zea mays) have been named cnr (cell number regulator) and two of them exert their effect on organ size by modulating cell number ...201323976873
conventional and ultrasound-assisted extraction of anthocyanins from blackberry and sweet cherry cultivars.blackberry and sweet cherry are important plant foods rich in anthocyanins well-known for their pharmacological and antioxidant effects. the aim of the present paper was to comparatively investigate conventional and ultrasound-assisted extraction procedures in order to isolate an enriched crude anthocyanin extract from blackberry (thornfree cultivar) and sweet cherry (black gold cultivar). hydroethanolic solution and acidified ethanol were used to conventionally extract anthocyanins by a discont ...201323878943
non-targeted 1h nmr fingerprinting and multivariate statistical analyses for the characterisation of the geographical origin of italian sweet cherries.in this study, non-targeted (1)h nmr fingerprinting was used in combination with multivariate statistical techniques for the classification of italian sweet cherries based on their different geographical origins (emilia romagna and puglia). as classification techniques, soft independent modelling of class analogy (simca), partial least squares discriminant analysis (pls-da), and linear discriminant analysis (lda) were carried out and the results were compared. for lda, before performing a refine ...201323871055
comparative genomics analysis in prunoideae to identify biologically relevant polymorphisms.prunus is an economically important genus with a wide range of physiological and biological variability. using the peach genome as a reference, sequencing reads from four almond accessions and one sweet cherry cultivar were used for comparative analysis of these three prunus species. reference mapping enabled the identification of many biological relevant polymorphisms within the individuals. examining the depth of the polymorphisms and the overall scaffold coverage, we identified many potential ...201323763653
measurement of antioxidant activity and antioxidant compounds under versatile extraction conditions: ii. the immuno-biochemical antioxidant properties of black sour cherry (prunus cerasus) extracts.retrospectively, we have measured the antioxidant activity and a variety of antioxidant compounds under versatile extraction conditions of sweet cherry (prunus avium) extracts. further in this study, in order to understand the biochemical constituents and antioxidant activities of a variety of extracts of black sour cherries (p. cerasus), a related species, antioxidant compounds, including l-ascorbic acid (vitamin c), phenols, flavonoids, and anthocyanins, and the total antioxidant activity were ...201323651230
evaluating the potential of artificial neural network and neuro-fuzzy techniques for estimating antioxidant activity and anthocyanin content of sweet cherry during ripening by using image processing.this paper presents a versatile way for estimating antioxidant activity and anthocyanin content at different ripening stages of sweet cherry by combining image processing and two artificial intelligence (ai) techniques. in comparison with common time-consuming laboratory methods for determining these important attributes, this new way is economical and much faster. the accuracy of artificial neural network (ann) and adaptive neuro-fuzzy inference system (anfis) models was studied to estimate the ...201423633396
inheritance of hetero-diploid pollen s-haplotype in self-compatible tetraploid chinese cherry (prunus pseudocerasus lindl).the breakdown of self-incompatibility, which could result from the accumulation of non-functional s-haplotypes or competitive interaction between two different functional s-haplotypes, has been studied extensively at the molecular level in tetraploid rosaceae species. in this study, two tetraploid chinese cherry (prunus pseudocerasus) cultivars and one diploid sweet cherry (prunus avium) cultivar were used to investigate the ploidy of pollen grains and inheritance of pollen-s alleles. genetic an ...201323596519
melatonin and its potential biological functions in the fruits of sweet cherry.melatonin is a well-known molecule which possesses many beneficial effects on human health. many agriculture products provide natural melatonin in the diet. cherry is one such fruit as they are rich in melatonin. in order to understand the biological roles of melatonin in cherry fruit, melatonin synthesis and its changes over 24 hr period were systematically monitored both during their development and in the ripe cherries in two cultivars, 'hongdeng' (prunus avium l. cv. hongdeng) and 'rainier' ...201323480341
construction and comparative analyses of highly dense linkage maps of two sweet cherry intra-specific progenies of commercial cultivars.despite the agronomical importance and high synteny with other prunus species, breeding improvements for cherry have been slow compared to other temperate fruits, such as apple or peach. however, the recent release of the peach genome v1.0 by the international peach genome initiative and the sequencing of cherry accessions to identify single nucleotide polymorphisms (snps) provide an excellent basis for the advancement of cherry genetic and genomic studies. the availability of dense genetic link ...201323382953
s genotyping in japanese plum and sweet cherry by allele-specific hybridization using streptavidin-coated magnetic beads.we report a rapid and reliable method for s genotyping of rosaceae fruit trees, which would to be useful for successful planting of cross-compatible cultivars in orchards. japanese plum (prunus salicina) and sweet cherry (prunus avium), belonging to the family rosaceae, possess gametophytic self-incompatibility controlled by a single polymorphic locus containing at least two linked genes, s-rnase and sfb (s-haplotype-specific f-box gene). for successful planting of cross-compatible cultivars of ...201323338476
development and evaluation of a genome-wide 6k snp array for diploid sweet cherry and tetraploid sour cherry.high-throughput genome scans are important tools for genetic studies and breeding applications. here, a 6k snp array for use with the illumina infinium® system was developed for diploid sweet cherry (prunus avium) and allotetraploid sour cherry (p. cerasus). this effort was led by rosbreed, a community initiative to enable marker-assisted breeding for rosaceous crops. next-generation sequencing in diverse breeding germplasm provided 25 billion basepairs (gb) of cherry dna sequence from which wer ...201223284615
molecular and quantitative signatures of biparental inbreeding depression in the self-incompatible tree species prunus avium.genetic diversity strongly influences populations' adaptability to changing environments and therefore survival. sustainable forest management practices have multiple roles including conservation of genetic resources and timber production. in this study, we aimed at better understanding the variation in genetic diversity among adult and offspring individuals, and the effects of mating system on offspring survival and growth in wild cherry, prunus avium. we analysed adult trees and open pollinate ...201323211795
micropropagation of prunus species relevant to cherry fruit production.cherry tree micropropagation is limited to the production of healthy cultivars of prunus avium and prunus cerasus, and their rootstocks; mainly the dwarfing ones. by using meristem-tip (0.1 mm long) or healthy shoot tips/nodes, four successive steps are needed to obtain whole plants capable of growing in the nursery: multiplication by axillary branching, shoot elongation, rooting, and plantlet acclimation. along this process, several parameters have to be adjusted for each phase of the culture, ...201323179694
melatonin enhances root regeneration, photosynthetic pigments, biomass, total carbohydrates and proline content in the cherry rootstock phl-c (prunus avium × prunus cerasus).the present study, investigates the effects of melatonin (0, 0.05, 0.1, 0.5, 1, 5 and 10 μm) on the morphogenic and biochemical responses in the cherry rootstock phl-c (prunus avium l. × prunus cerasus l.), from shoot tip explants. the incorporation of melatonin (0-10 μm) in the murashige and skoog (ms) medium, greatly influenced rooting either positively or negatively. melatonin, irrespective of its concentration, had a negative effect concerning the number of roots. however, application of 0.5 ...201223127522
purification and chemical characterisation of a cell wall-associated β-galactosidase from mature sweet cherry (prunus avium l.) fruit.using four different chromatographic steps, β-galactosidase was purified from the ripe fruit of sweet cherry to apparent electrophoretic homogeneity with approximately 131-fold purification. the prunus avium β-galactosidase showed an apparent molecular mass of about 100 kda and consisted of four different active polypeptides with pis of about 7.9, 7.4, 6.9 and 6.4 as estimated by native ief and β-galactosidase-activity staining. the active polypeptides were individually excised from the gel and ...201223121861
formulation of indomethacin emulsion using biopolymer of prunus avium.the aim of the investigation was to formulate indomethacin emulsion using bio-polymer as emulsifier. different batches of emulsions were prepared by varying concentration of biopolymer prunus avium. based evaluation of the prepared polymers, a conclusion can be drawn that in the prunus avium bio-material can serve as a promising film forming agent for formulating various drug.201223066192
genetic variability of wild cherry (prunus avium l.) seed stands in slovenia as revealed by nuclear microsatellite loci.microsatellite markers were used to describe the genetic variability of four seed stands of wild cherry (prunus avium l.). one hundred and thirty one individuals were genotyped at ten nuclear microsatellite loci. total genetic diversity was high (h(e) = 0.704), while differences between stands were small but significant (f(st) = 0.053, g'(st) = 0.234). there was a significant amount of clonal reproduction in one stand, with only 11 genotypes identified among 36 trees. one stand showed a signific ...201222911762
development and cross-species/genera transferability of microsatellite markers discovered using 454 genome sequencing in chokecherry (prunus virginiana l.).chokecherry (prunus virginiana l.) (2n = 4x = 32) is a unique prunus species for both genetics and disease-resistance research due to its tetraploid nature and x-disease resistance. however, no genetic and genomic information on chokecherry is available. a partial chokecherry genome was sequenced using roche 454 sequencing technology. a total of 145,094 reads covering 4.8 mbp of the chokecherry genome were generated and 15,113 contigs were assembled, of which 11,675 contigs were larger than 100  ...201222837059
comparison of the genetic determinism of two key phenological traits, flowering and maturity dates, in three prunus species: peach, apricot and sweet cherry.the present study investigates the genetic determinism of flowering and maturity dates, two traits highly affected by global climate change. flowering and maturity dates were evaluated on five progenies from three prunus species, peach, apricot and sweet cherry, during 3-8 years. quantitative trait locus (qtl) detection was performed separately for each year and also by integrating data from all years together. high heritability estimates were obtained for flowering and maturity dates. several q ...201222828898
genetic diversity and relatedness of sweet cherry (prunus avium l.) cultivars based on single nucleotide polymorphic markers.most previous studies on genetic fingerprinting and cultivar relatedness in sweet cherry were based on isoenzyme, rapd, and simple sequence repeat (ssr) markers. this study was carried out to assess the utility of single nucleotide polymorphism (snp) markers generated from 3' untranslated regions (utr) for genetic fingerprinting in sweet cherry. a total of 114 sweet cherry germplasm representing advanced selections, commercial cultivars, and old cultivars imported from different parts of the wor ...201222737155
soluble sugars and sucrose-metabolizing enzymes related to cold acclimation of sweet cherry cultivars grafted on different rootstocks.the bark tissues were collected from 4-year-old sweet cherry trees cvs. 0900 ziraat and lambert grafted on gisela 5 and mazzard rootstocks in cold-acclimated (ca) and nonacclimated (na) stages. bark tissues subjected to 4 °c and -5 °c injured to a limited extent in both stages. however, more than 50% injury occurred by temperatures equal to or colder than -15 °c only in na period. total soluble sugar (tss), reducing sugars, and sucrose contents were higher in ca than those in na stages in all sa ...201222629228
comparative study of phenolic compounds and antioxidant activity in different species of cherries.a new spectrometric method ultra performance liquid chromatography-tandem mass spectrometric with high precision and rapid analysis was developed to separate 17 phenolic compounds. different species of cherries, including 10 sweet cherry (prunus avium l.) cultivars, a tart cherry (p. cerasus l.) rootstock (cab), and a hybrid rootstock 'colt' (p. avium × p. pseudocerasus), were analyzed for phenolics contents by this method. the results showed that significant differences were observed among the ...201122417346
compositional variation in sugars and organic acids at different maturity stages in selected small fruits from pakistan.selected soluble sugars and organic acids were analyzed in strawberry, sweet cherry, and mulberry fruits at different ripening stages by hplc. the amounts of fructose, glucose and sucrose were found to be: strawberry (1.79-2.86, 1.79-2.25 and 0.01-0.25 g/100 g fw), sweet cherry (0.76-2.35, 0.22-3.39 and 0.03-0.13 g/100 g) and mulberry (3.07-9.41, 1.53-4.95 and 0.01-0.25 g/100 g) at un-ripened to fully-ripened stages, respectively. the strawberry, sweet cherry and mulberry mainly contained tartar ...201222408396
developing drosophila suzukii management programs for sweet cherry in the western united states.the spotted wing drosophila, drosophila suzukii matsumura (diptera: drosophilidae), is a newly introduced pest of sweet cherry on the west coast of north america which produces about 97% of the value of the us sweet cherry crop. d. suzukii initially caused considerable economic loss to cherry growers, who were unaware of this new pest. little control information was available at the time of initial infestation. pest control studies were initiated to examine the materials, timings and application ...201121919187
phosphoenolpyruvate carboxykinase in cherry (prunus avium l.) fruit during development.in this study the abundance and location of phosphoenolpyruvate carboxykinase (pepck) was determined in the flesh and skin of the sweet cherry (prunus avium l.) cultivar durone nero ii during development. pepck was not present in young fruit but appeared in both tissues as the fruit increased in size. in these there was no net dissimilation of malic acid, which accounts for the bulk of their organic acid contents when pepck was present. to assist in understanding the function of pepck, the abund ...201121908472
melatonin promotes adventitious root regeneration in in vitro shoot tip explants of the commercial sweet cherry rootstocks cab-6p (prunus cerasus l.), gisela 6 (p. cerasus × p. canescens), and mxm 60 (p. avium × p. mahaleb).the objectives of this study were to test the effects of melatonin (n-acetyl-5-methoxytryptamine), a natural compound of edible plants on the rooting of certain commercial sweet cherry rootstocks. shoot tip explants from previous in vitro cultures of the cherry rootstocks cab-6p (prunus cerasus l.), gisela 6 (p. cerasus × p. canescens), and m × m 60 (p. avium × p. mahaleb) were included in the experiment. the effect of indole-3-acetic acid (iaa) and indole-3-butyric acid (iba) alone or in combin ...201221749439
metabolism of the seed and endocarp of cherry (prunus avium l.) during development.in this study some aspects of organic and amino acid metabolism in cherry endocarp and seed were investigated during their development. the abundance and location of a number of enzymes involved in these processes were investigated. these enzymes were aspartate aminotransferase (aspat; ec:2.6.1.1), glutamine synthetase (gs; ec:6.3.1.2), phosphoenolpyruvate carboxylase (pepc; ec:4.1.1.31), phosphoenolpyruvate carboxykinase (pepck; ec:4.1.1.49), and ribulose-1,5-bisphosphate carboxylase/oxygenase ...201121741262
postharvest treatments with salicylic acid, acetylsalicylic acid or oxalic acid delayed ripening and enhanced bioactive compounds and antioxidant capacity in sweet cherry.sweet cherry cultivars ('cristalina' and 'prime giant') harvested at commercial ripening stage were treated with salicylic acid (sa), acetylsalicylic acid (asa) or oxalic acid (oa) at 1 mm and then stored for 20 days under cold temperature. results showed that all treatments delayed the postharvest ripening process, manifested by lower acidity, color changes and firmness losses, and maintained quality attributes for longer periods than controls. in addition, total phenolics, anthocyanins and ant ...201121506518
analysis of anthocyanins in commercial fruit juices by using nano-liquid chromatography-electrospray-mass spectrometry and high-performance liquid chromatography with uv-vis detector.nano-lc and conventional hplc techniques were applied for the analysis of anthocyanins present in commercial fruit juices using a capillary column of 100 μm id and a 2.1 mm id narrow-bore c(18) column. analytes were detected by uv-vis at 518 nm and esi-ion trap ms with hplc and nano-lc, respectively. commercial blueberry juice (14 anthocyanins detected) was used to optimize chromatographic separation of analytes and other analysis parameters. qualitative identification of anthocyanins was perfor ...201121246720
characterization and mapping of non-s gametophytic self-compatibility in sweet cherry (prunus avium l.).self-incompatibility in prunus (rosaceae) species, such as sweet cherry, is controlled by a multiallelic locus (s), in which two tightly linked genes, s-rnase and sfb (s haplotype-specific f-box), determine the specificity of the pollen and the style. fertilization in these species occurs only if the s-specificities expressed in the pollen and the pistils are different. however, modifier genes have been proposed to be necessary for a full manifestation of the self-incompatibility response. 'cris ...201121127024
effect of the commercial ripening stage and postharvest storage on microbial and aroma changes of 'ambrunés' sweet cherries.the purpose of this work was to investigate the effect of the commercial ripening stage and postharvest storage on microbial and aroma changes of 'ambrunés' sweet cherries. the microbial counts and volatile profile of sweet cherry batches automatically sanitized and classified in three commercial ripening stages were studied for five postharvest storages. the batches were also evaluated sensorially, and the correlations between volatile compounds and aroma quality were determined. the microbial ...201023654239
cloning and expression analysis of cdnas for aba 8'-hydroxylase during sweet cherry fruit maturation and under stress conditions.abscisic acid (aba) plays a key role in various aspects of plant growth and development, including adaptation to environmental stress and fruit maturation in sweet cherry fruit. in higher plants, the level of aba is determined by synthesis and catabolism. in order to gain insight into aba synthesis and catabolism in sweet cherry fruit during maturation and under stress conditions, four cdnas of paccyp707a1 -paccyp707a4 for 8'-hydroxylase, a key enzyme in the oxidative catabolism of aba, and one ...201020728961
population structure and genetic bottleneck in sweet cherry estimated with ssrs and the gametophytic self-incompatibility locus.domestication and breeding involve the selection of particular phenotypes, limiting the genomic diversity of the population and creating a bottleneck. these effects can be precisely estimated when the location of domestication is established. few analyses have focused on understanding the genetic consequences of domestication and breeding in fruit trees. in this study, we aimed to analyse genetic structure and changes in the diversity in sweet cherry prunus avium l.201020727153
chitosan acetate as an active coating material and its effects on the storing of prunus avium l.in this article, chitosan acetate (ca) was prepared by the method of solid-liquid reaction. ca was a stable faint yellow powder with water solubility. ca kept the same backbone in the chemical structure as the raw material of chitosan, and it also had the similar antibacterial properties with chitosan. ca could form a coating film on the outside surface of the sweet cherries, could effectively retard the loss of the water, titratable acidity, and ascorbic acid of sweet cherries, and could induce ...201020492258
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