| performance evaluation of puritan® universal transport system (unitranz-rt™) for preservation and transport of clinical viruses. | the ability of a non-propagating microbial transport medium to maintain the viability of clinically relevant viruses was compared to a similar commercial medium to establish performance equivalence. two dilutions of stock of test viruses, namely adenovirus (adv), cytomegalovirus (cmv), echovirus type 30 (ev), herpes simplex virus (hsv) types 1 and 2, influenza a, parainfluenza 3 (piv), respiratory syncytial virus (rsv), and varicella zoster virus (vzv), were spiked into puritan® medical products ... | 2015 | 26243168 |
| echovirus serotypes circulating in malaysia from 2002 to 2013. | to identify the circulating serotypes of human echovirus in malaysia from 2002 to 2013. | 2016 | 26972396 |
| [genomic characteristics of an echovirus 20 strain (km/ev20/2010) isolated in kunming, yunnan, china]. | to identify the complete genome sequence of an echovirus 20 isolate (km/ev20/2010) and understand its genetic variation and evolution characteristics. | 2015 | 26080642 |
| comparative analysis of viral shedding in pediatric and adult subjects with central nervous system-associated enterovirus infections from 2013 to 2015 in switzerland. | several enterovirus (ev) genotypes can result in aseptic meningitis, but their routes of access to the central nervous system remain to be elucidated and may differ between the pediatric and adult populations. | 2017 | 28214758 |
| sequential transmigration of polymorphonuclear cells and naive cd3(+) t lymphocytes across the blood-cerebrospinal-fluid barrier in vitro following infection with echovirus 30. | viral meningitis by non-polio enteroviruses (npev) is a major public health burden causing fatal outcomes especially in the younger population. strong evidence exists that the blood-cerebrospinal-fluid (csf) barrier (bcsfb) serves as an entry point for enterovirus and leucocytes into the central nervous system (cns). moreover, analysis of clinical csf specimens of patients with a npev infection revealed a predominance of polymorphonuclear granulocytes (pmn) in the early phase and mononuclear cel ... | 2017 | 28161477 |
| seroepidemiological and phylogenetic characterisation of neurotropic enteroviruses in ireland, 2005-2014. | enteroviruses (evs) are associated with a broad spectrum of clinical presentation, including aseptic meningitis (am), encephalitis, hand, foot and mouth disease, acute flaccid paralysis and acute flaccid myelitis. epidemics occur sporadically and are associated with increased cases of am in children. the present study describes the seroepidemiological analysis of circulating evs in ireland from 2005-2014 and phylogenetic characterisation of echovirus 30 (e-30), enterovirus a71 (ev-a71) and enter ... | 2017 | 28071799 |
| a large finnish echovirus 30 outbreak was preceded by silent circulation of the same genotype. | an outbreak of echovirus 30 (e-30) in 2009 was confirmed by both frequent isolation of the virus from sewage as well as from patient samples in finland. over the last 10 years e-30 had only been isolated sporadically in finland. we here study the phylogenetic relationships of the strains from the outbreak in the context of e-30 circulation over the last 20 years. the analyzed region comprised 276 nucleotides in the 5' end of vp1 (nucleotides 132-407 in the vp1 of the e-30 bastianni strain). the ... | 2010 | 20960045 |
| phylogenetic analysis of echovirus 30 isolated during the 2005 outbreak in france reveals existence of multiple lineages and suggests frequent recombination events. | echovirus 30 (e-30) was responsible in france for a major aseptic meningitis outbreak during 2005 summer season. however, the virological mechanisms responsible for the periodic emergence of the epidemic strains remain to be investigated. | 2010 | 20381415 |
| clinical and virological features of an aseptic meningitis outbreak in north-eastern france, 2005. | enteroviruses (evs) are considered as a major viral etiological cause of aseptic meningitis in children. | 2008 | 18358773 |
| three cases of mumps virus and enterovirus coinfection in children with enteroviral meningitis. | several viruses are responsible for aseptic meningitis; however, in the region of southwest iran, the role played by each virus is still not very well known. the aim of this study is to determine the relative frequencies of mumps virus, herpes viruses, and enteroviruses, as well as coinfections among them, in patients with aseptic meningitis.in this cross-sectional study, samples of cerebrospinal fluid were collected between december 2012 and december 2013 from patients under 14 years, who were ... | 2016 | 27930588 |
| [epidemiology, etiology and clinical picture of enteroviral meningitis in children in south moravia]. | enteroviruses (evs) are the most common cause of aseptic viral meningitis. in some cases, they can cause severe meningoencephalitis and acute flaccid paralysis - an association with some virulent serotypes. the objectives were to describe the epidemiological situation of ev meningitis in children in south moravia, to elucidate the etiology including the incidence of virulent serotypes and to evaluate the clinical presentation. | 2016 | 27907970 |
| laboratory diagnosis and genetic analysis of a family clustering outbreak of aseptic meningitis due to echovirus 30. | echovirus 30 (e30) is a major pathogen associated with aseptic meningitis. in the summer of 2014, a family clustering aseptic meningitis outbreak occurred in urban-rural fringe of ningbo city in zhejiang province in china. to identify the etiologic agent, specimens were tested by cell culture and reverse transcriptase-polymerase chain reaction. pathogenic examination confirmed that the outbreak is caused by e30. the first case is a 6-year-old child, who studied in kindergarten in local, suffered ... | 2016 | 27646838 |
| predominance of enterovirus b and echovirus 30 as cause of viral meningitis in a uk population. | enteroviruses are the most common cause of aseptic or lymphocytic meningitis, particularly in children. with reports of unusually severe neurological disease in some patients infected with enterovirus d68 in north america, and a recent increase in the number of paediatric enterovirus meningitis cases presenting in this uk midlands population, a retrospective regional surveillance study was performed. | 2016 | 27367546 |
| molecular characterization of echovirus 30 isolates from poland, 1995-2015. | echovirus 30 (e30) is one of the most frequently identified enterovirus and a major cause of meningitis in children and adults. to investigate the genetic variability and relationship of e30 isolated from specimens of aseptic meningitis cases that occurred in poland over a period of 20 years, sequences of vp1 gene were determined and genetic analysis was performed. from 1995 to 2015, 124 e30 were isolated using rd cells, and 58 isolates were sequenced and characterized by phylogenetic analysis o ... | 2016 | 26957092 |
| echovirus 30 meningitis epidemic followed by an outbreak-specific rt-qpcr. | an outbreak of enteroviral aseptic meningitis emerged in southwestern finland in august 2009. the same enterovirus reappeared with increasing incidence of meningitis in other parts of finland in 2010. | 2015 | 26209368 |
| lower anti-echovirus antibody responses in children presenting to hospital with asthma exacerbations. | rhinoviruses from the enterovirus genus cause frequent infections and induce remarkably high titres of anticapsid antigen antibodies in asthmatics, while the prevalence of neutralising antibodies to the gut-trophic echoviruses from the same genus is diminished. | 2015 | 25640320 |
| elucidation of echovirus 30's origin and transmission during the 2012 aseptic meningitis outbreak in guangdong, china, through continuing environmental surveillance. | an aseptic meningitis outbreak occurred in luoding city of guangdong, china, in 2012, and echovirus type 30 (echo30) was identified as the major causative pathogen. environmental surveillance indicated that echo30 was detected in the sewage of a neighboring city, guangzhou, from 2010 to 2012 and also in luoding city sewage samples (6/43, 14%) collected after the outbreak. in order to track the potential origin of the outbreak viral strains, we sequenced the vp1 genes of 29 viral strains from cli ... | 2015 | 25616804 |
| aseptic meningitis outbreak associated with echovirus 30 among high school football players--los angeles county, california, 2014. | on august 4, 2014, the acute communicable disease control program of the los angeles county department of public health received a report of three aseptic meningitis cases among football players at a county high school. an investigation was conducted to determine the extent of the outbreak, identify potential exposures, and recommend control measures. an outbreak-associated aseptic meningitis case was defined as an illness of any team or family member with onset during july 28-august 11 with 1) ... | 2015 | 25551596 |
| widespread circulation of a new echovirus 30 variant causing aseptic meningitis and non-specific viral illness, south-east france, 2013. | human enteroviruses (hevs) are major cause of aseptic meningitis. a new outbreak of e-30 occurred between april and september 2013 in marseille, south-east france. | 2014 | 24973284 |
| aseptic meningitis outbreak caused by echovirus 30 in two regions in bulgaria, may-august 2012. | an aseptic meningitis outbreak emerged in two regions in bulgaria in 2012 and echovirus 30 (e30) was established as the aetiological agent by cell culture isolation, serological test, and molecular-based techniques. a total of 157 patients with aseptic meningitis were investigated, of which 117 were confirmed as having e30-associated disease. molecular analysis of 12 e30 isolates revealed 99-100% nucleotide and amino-acid identity between them and a close correlation with a greek strain involved ... | 2014 | 24480099 |
| increased levels of cytokines in cerebrospinal fluid of children with aseptic meningitis caused by mumps virus and echovirus 30. | we measured levels of pro-inflammatory cytokines in the cerebrospinal fluid (csf) of patients with mumps meningitis, enteroviral echovirus 30 meningitis and children without central nervous system infection to investigate whether these molecules were involved in the pathogenesis of viral meningitis. the csf was obtained from 62 children suspected with meningitis. these patients were classified to the mumps meningitis (n = 19), echovirus 30 meningitis (n = 22) and non-meningitis (n = 21) groups. ... | 2014 | 24313836 |
| recent outbreak of aseptic meningitis in italy due to echovirus 30 and phylogenetic relationship with other european circulating strains. | enteroviruses (evs) are common human viral pathogens, causing a variety of diseases, including aseptic meningitis. recently, ev aseptic meningitis outbreaks have been reported across europe, but, in italy, knowledge of recent ev molecular epidemiology is very limited. | 2013 | 24051044 |
| complete genome sequence analysis of human echovirus 30 isolated during a large outbreak in guangdong province of china, in 2012. | in may and june 2012, an outbreak of aseptic meningitis caused by echovirus 30 (e30) occurred on a large scale in luoding, guangdong province, china. our team successfully isolated one subtype, strain 2012em161, and its complete genome was sequenced. the phylogenetic tree of viral protein (vp) 1 gene sequences showed that the viral isolate was similar to the e30 strain prevalent in fujian (2011), with identity of 98.05-99.32 % and 98.63-99.32 % for nucleotides and amino acids respectively. whole ... | 2014 | 23990054 |
| molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in guangdong in 2012. | evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. an outbreak of aseptic meningitis occurred from may to june, 2012, in guangdong province, china. in order to determine the etiologic agent, csf specimens from 121 children hospitalized for aseptic meningitis at luoding people's hospital of guangdong province were tested for virus isolation and identification. | 2013 | 23968330 |
| molecular characterization of enteroviruses associated with neurological infections in spain, 2008. | in order to investigate the etiology of viral neurological infections in spain, a national study was performed in 2008. the results obtained have been published. enteroviruses were the most frequent cause of the aseptic meningitis and infant febrile syndromes. the present report supplements the previous study with the genotyping of the detected enteroviruses. typing was by amplification of partial vp1 region and sequencing in 70 (53%) of the 132 available cerebrospinal fluid samples positive for ... | 2013 | 23893817 |
| genetic characterization of ev71 isolates from 2004 to 2010 reveals predominance and persistent circulation of the newly proposed genotype d and recent emergence of a distinct lineage of subgenotype c2 in hong kong. | enterovirus 71 (ev71) is a common etiological agent of hand, foot and mouth disease (hfmd) in children. ev71 epidemics have been reported in hong kong in recent years, and yet the genetic information of ev71 strains circulating in our locality is limited. the objective of this study was to investigate the genetic evolution of these ev71 isolates in hong kong over a 7-year period. | 2013 | 23822185 |
| echovirus 30 outbreak associated with a high meningitis attack rate in thrace, greece. | between late may and july 2012, 105 children (62 boys) originating from 2 cities of thrace were examined because of fever, headache and abdominal pain. thirty-three of them were hospitalized. they had normal hemograms, and mild to moderate cerebrospinal fluid pleocytosis. echovirus 30 was isolated from fecal and cerebrospinal fluid samples. among confirmed cases of echoviral illness, the meningitis attack rate was 51.9%. | 2013 | 23459085 |
| molecular epidemiology of echovirus 30 in fujian, china between 2001 and 2011. | echovirus 30 (e-30) was responsible for an outbreak of aseptic meningitis between april 1 and june 2, 2011 in fujian province, china. a molecular epidemiology study of 115 e-30 strains was performed to characterize the genetic features of the etiologic agent of the 2011 aseptic meningitis outbreak. the phylogenetic trees of the complete vp1 gene (876 bp) from 74 of 115 isolates and 50 reference sequences were analyzed. three lineages (e-30_h, i, and j) were detected that had co-circulated in fuj ... | 2013 | 23359298 |
| molecular diagnosis of echovirus 30 as the etiological agent in an outbreak of aseptic meningitis in panama: may-june 2008. | aseptic meningitis outbreaks are commonly caused by viral pathogens with enterovirus a common etiological agent. between may and june of 2008, an outbreak of 173 cases of aseptic meningitis occurred in the chiriqui province of panama. molecular techniques were used to identify the etiological agent. | 2012 | 23276736 |
| complete genome sequence analysis of human echovirus type 30 isolated in china. | we report here the complete genome sequence of a human echovirus type 30 strain ecv30/gx10/05 isolated in guangxi, china, in 2010. phylogenetic analysis showed that ecv30/gx10/05 was closely related to a korean strain isolated in 2008. the sequence information will help in an understanding of the molecular epidemiology and evolution of echovirus. | 2012 | 23166258 |
| chemotaxis of t-cells after infection of human choroid plexus papilloma cells with echovirus 30 in an in vitro model of the blood-cerebrospinal fluid barrier. | enterovirus is the most common pathogen causing viral meningitis especially in children. besides the blood-brain barrier (bbb) the choroid plexus, which forms the blood-cerebrospinal-fluid (csf) barrier (bcsfb), was shown to be involved in the pathogenesis of enteroviral meningitis. in a human in vitro model of the bcsfb consisting of human choroid plexus papilloma cells (hibcpp), the permissiveness of plexus epithelial cells for echovirus 30 (ev30) was analyzed by immunoblotting and quantitativ ... | 2012 | 23000117 |
| quantitative real-time rt-pcr assay for research studies on enterovirus infections in the central nervous system. | human enteroviruses are the most frequent cause of aseptic meningitis and are involved in other neurological infections. qualitative detection of enterovirus genomes in cerebrospinal fluid is a prerequisite in diagnosing neurological diseases. the pathogenesis of these infections is not well understood and research in this domain would benefit from the availability of a quantitative technique to determine viral load in clinical specimens. this study describes the development of a real-time rt-qp ... | 2012 | 22766179 |
| echovirus 30 induced neuronal cell death through trio-rhoa signaling activation. | echovirus 30 (echo30) is one of the most frequently identified human enteroviruses (evs) causing aseptic meningitis and encephalitis. however the mechanism underlying the pathogenesis of echo30 infection with significant clinical outcomes is not completely understood. the aim of this investigation is to illustrate molecular pathologic alteration in neuronal cells induced by echo30 infection using clinical isolate from young patient with neurologic involvement. | 2012 | 22586486 |
| [study on the pathological and molecular characteristics of viral meningitis outbreaks in linhai county, zhejiang province, 2004]. | in order to confirm the causes of viral meningitis outbreaks in linhai county, zhejiang province in 2004, and to analyze the relationship between hereditary variation and evolution of the pathogen. | 2011 | 22336614 |