| a multi-subunit chlamydial vaccine induces antibody and cell-mediated immunity in immunized koalas (phascolarctos cinereus): comparison of three different adjuvants. | chlamydial infections represent a major threat to the survival of the koala. infections caused by chlamydia pecorum cause blindness, infertility, pneumonia and urinary tract infections and represent a threat to the survival of the species. little is known about the immune response in koalas, or the safety of commonly used adjuvants for induction of protective systemic and mucosal immunity. | 2010 | 20064144 |
| characterization of multidrug-resistant escherichia coli isolated from extraintestinal clinical infections in animals. | multidrug-resistant (mdr) escherichia coli causes extraintestinal infections in both humans and animals. this study aimed to determine whether mdr e. coli isolates cultured from extraintestinal infections in several animal species were clonal and crossed host-species boundaries, as suggested by initial characterization of a subset of canine and human isolates, or whether they represented a diverse group of host-specific strains. isolates were obtained either from the university of queensland vet ... | 2010 | 20093377 |
| host-microbial interactions in childhood atopy: toll-like receptor 4 (tlr4), cd14, and fecal escherichia coli. | perturbations in the gut microbiota have been linked to atopic diseases. however, the development of atopic diseases depends not only on environmental factors (like microbial stimulation) but also on genetic factors. it is likely that particularly gene-environmental interactions in early life determine the development of atopy. | 2010 | 20109750 |
| assessment of the hemorheological profile of koala and echidna. | koala, a marsupial, and echidna, a monotreme, are mammals native to australia. blood viscosity (62.5-1250s(-1)), red blood cell (rbc) deformability, rbc aggregation, aggregability and surface charge, and hematological parameters were measured in blood samples from six koalas and six echidnas and compared to adult human blood. koala had the largest rbc mean cell volume (107.7+/-2.6fl) compared to echidna (81.3+/-2.6fl) and humans (88.4+/-1.2fl). echidna blood exhibited the highest viscosity over ... | 2010 | 20138490 |
| trans fatty acids in human milk are an indicator of different maternal dietary sources containing trans fatty acids. | the trans fatty acid (tfa) patterns in the fats of ruminant meat and dairy products differ from those found in other (processed) fats. we have evaluated different tfa isomers in human breast milk as an indicator of dietary intake of ruminant and dairy fats of different origins. breast milk samples were collected 1 month postpartum from 310 mothers participating in the koala birth cohort study (the netherlands). the study participants had different lifestyles and consumed different amounts of dai ... | 2010 | 20148367 |
| the interfacial viscoelastic properties and structures of human and animal meibomian lipids. | as the interface between the aqueous layer of the tear film and air, the lipid layer plays a large role in maintaining tear film stability. meibomian lipids are the primary component of the lipid layer; therefore the physical properties of these materials may be particularly crucial to the functionality of the tear film. surface pressure versus area isotherms, interfacial shear and extensional rheology, and brewster angle microscopy (bam) were used to characterize the meibomian lipids from diffe ... | 2010 | 20156438 |
| fads1 fads2 gene variants modify the association between fish intake and the docosahexaenoic acid proportions in human milk. | the genes encoding delta(5)- and delta(6)-desaturases (fads1 fads2 gene cluster) were reported to be associated with n-3 (omega-3) and n-6 (omega-6) fatty acid proportions in human plasma, tissues, and milk. docosahexaenoic acid (dha) can be supplied especially by dietary fish or fish oil and synthesized from alpha-linolenic acid through a pathway involving these desaturases. | 2010 | 20335541 |
| immunoproteomic approach to elucidating the pathogenesis of cryptococcosis caused by cryptococcus gattii. | cryptococcosis caused by cryptococcus gattii is a devastating disease of immunocompetent hosts with an incompletely understood pathogenesis. utilizing an immunoproteomic approach in a naturally occurring koala model of disease, a number of key proteins and pathways are identified in the early and late pathogenesis of cryptococcosis for the first time. in particular, the thioredoxin system appears important in the pathogenesis of cryptococcosis caused by c. gattii vgii. | 2010 | 20545298 |
| comparison of koala lpcoln and human strains of chlamydia pneumoniae highlights extended genetic diversity in the species. | chlamydia pneumoniae is a widespread pathogen causing upper and lower respiratory tract infections in addition to a range of other diseases in humans and animals. previous whole genome analyses have focused on four essentially clonal (> 99% identity) c. pneumoniae human genomes (ar39, cwl029, j138 and tw183), providing relatively little insight into strain diversity and evolution of this species. | 2010 | 20646324 |
| fatty acids in breast milk and development of atopic eczema and allergic sensitisation in infancy. | one of the explanations for the increasing prevalence of atopic diseases is a relative low perinatal supply of n-3 fatty acids. however, this does not explain the protective effects of whole-fat dairy products or high levels of transfatty acids in breast milk, observed in some studies. we evaluated the role of perinatal supply of fatty acids in the early development of atopic eczema and allergic sensitisation. | 2011 | 20659079 |
| novel trypanosome trypanosoma gilletti sp. (euglenozoa: trypanosomatidae) and the extension of the host range of trypanosoma copemani to include the koala ( phascolarctos cinereus). | trypanosoma irwini was previously described from koalas and we now report the finding of a second novel species, t. gilletti, as well as the extension of the host range of trypanosoma copemani to include koalas. phylogenetic analysis at the 18s rdna and ggapdh loci demonstrated that t. gilletti was genetically distinct with a genetic distance (± s.e.) at the 18s rdna locus of 2.7 ± 0.5% from t. copemani (wombat). at the ggapdh locus, the genetic distance (± s.e.) of t. gilletti was 8.7 ± 1.1% fr ... | 2011 | 20663248 |
| isolation of koala retroviruses from koalas in japan. | koala retrovirus (korv) is considered to be associated with leukemia, lymphoma and immunodeficiency-like diseases in koalas. we therefore conducted a pilot study of korv infection in five queensland koalas in kobe municipal oji zoo. by polymerase chain reaction to detect partial env and pol genes of korv in genomic dna isolated from whole blood and feces, all five koalas were found to be positive for korv proviruses. we succeeded in culturing koala lymphocytes from less than 1 ml blood for over ... | 2011 | 20805639 |
| pulmonary cytochrome p450 enzymes belonging to the cyp4b subfamily from an australian marsupial, the tammar wallaby (macropus eugenii). | cytochromes p450 (cyps) are critically important in the oxidative metabolism of a diverse array of xenobiotics and endogenous substrates. we have previously reported the cloning and characterisation of the koala cyp4a15, the first reported member of the cyp4 family from marsupials, and have demonstrated important species differences in cyp4a activity and tissue expression. in the present study, the cloning of cyp4b1 in the wallaby (macropus eugenii) and their expression across marsupials is desc ... | 2011 | 20826229 |
| intestinal lactobacilli and the dc-sign gene for their recognition by dendritic cells play a role in the aetiology of allergic manifestations. | diminished exposure to harmless micro-organisms, such as lactobacilli, has been suggested to play a role in the increased prevalence of allergic disorders in westernized communities. the development of allergies depends on both environmental factors and genetic variations, including polymorphisms in genes encoding pattern recognition receptors. the present study examines the effects of both colonization with specific lactobacillus species and genetic variations in dc-sign, a pattern recognition ... | 2010 | 20829290 |
| increased neutralizing antibody response after simultaneous immunization with leucogen and the feline leukemia virus transmembrane protein. | to develop improved vaccination strategies against feline leukemia virus (felv), rats were immunized with the transmembrane envelope protein p15e of felv alone or in combination with the commercial vaccine leucogen® comprising the nonglycosylated felv surface envelope protein. binding and neutralizing antibodies were induced in both groups and in the group immunized with leucogen alone. higher titers of antibodies neutralizing felv were induced by simultaneous immunization with leucogen and p15e ... | 2011 | 20829603 |
| variants of the fads1 fads2 gene cluster, blood levels of polyunsaturated fatty acids and eczema in children within the first 2 years of life. | association of genetic-variants in the fads1-fads2-gene-cluster with fatty-acid-composition in blood of adult-populations is well established. we analyze this genetic-association in two children-cohort-studies. in addition, the association between variants in the fads-gene-cluster and blood-fatty-acid-composition with eczema was studied. | 2010 | 20948998 |
| candidatus bartonella antechini: a novel bartonella species detected in fleas and ticks from the yellow-footed antechinus (antechinus flavipes), an australian marsupial. | bartonella are fastidious, gram-negative, aerobic bacilli belonging to the alphaproteobacteria group. in the last ten years, the discovery of new bartonella species from a variety of mammalian hosts, arthropod vectors and geographical areas has increased. more than 20 species of bartonella have been identified, of which approximately thirteen are associated with disease in humans and animals. recently, four novel species of bartonella were isolated from mammalian hosts in australia: bartonella a ... | 2010 | 21215534 |
| cryptococcus yokohamensis sp. nov., a basidiomycetous yeast isolated from trees and queensland koala kept in a japanese zoological park. | three strains were isolated from the nostrils of a koala and the surrounding environment in a japanese zoological park. sequence analysis of the nuclear rdna internal transcribed spacer (its) region and the large subunit rrna gene d1/d2 domains in addition to physiological and morphological studies indicated that they represent a single novel species belonging to the basidiomycetous genus cryptococcus (tremellales, tremellomycetes, agaricomycotina). phylogenetic analysis based on d1/d2 and its r ... | 2011 | 21257688 |
| novel molecular markers of chlamydia pecorum genetic diversity in the koala (phascolarctos cinereus). | abstract: | 2011 | 21496349 |
| secretome of the coprophilous fungus doratomyces stemonitis c8, isolated from koala feces. | coprophilous fungi inhabit herbivore feces, secreting enzymes to degrade the most recalcitrant parts of plant biomass that have resisted the digestive process. consequently, the secretomes of coprophilous fungi have high potential to contain novel and efficient plant cell wall degrading enzymes of biotechnological interest. we have used one-dimensional and two-dimensional gel electrophoresis, matrix-assisted laser desorption ionization-time-of-flight tandem mass spectrometry (maldi-tof/tof ms/ms ... | 2011 | 21498763 |
| plasma concentrations of chloramphenicol after subcutaneous administration to koalas (phascolarctos cinereus) with chlamydiosis. | govendir, m., hanger, j., loader, j. j., kimble, b., griffith, j. e., black, l. a., krockenberger, m. b., higgins, d. p. plasma concentrations of chloramphenicol after subcutaneous administration to koalas (phascolarctos cinereus) with chlamydiosis. j. vet. pharmacol. therap. doi: 10.1111/j.1365-2885.2011.01307.x. nine mature koalas with chlamydiosis, typically keratoconjunctivitis and/or urogenital tract infection, were treated with daily subcutaneous injections of chloramphenicol at 60 mg/kg f ... | 2011 | 21569052 |
| detection and identification of probable endemic fungal pathogen, cryptococcus gattii, and worldwide pathogen, cryptococcus neoformans, by real-time pcr. | a real-time pcr method for detection and identification of cryptococcus neoformans and cryptococcus gattii was developed and evaluated using dna from single-colony or koala nasal smears. two taqman minor groove binder probes that distinguished between these species were designed corresponding to the internal sequences of the cap59 gene for both species. the real-time pcr assay had 100% specificity, as assessed using 13 reference strains and 300 environmental strains. twelve smear samples from he ... | 2011 | 21623899 |
| differential resistance of mammalian sperm chromatin to oxidative stress as assessed by a two-tailed comet assay. | protamines of eutherian species are cysteine-rich molecules that become cross-linked by disulfide bonds during epididymal transit, whereas the protamines of most marsupial species lack cysteine residuals. the present study made use of the differences in protamine structure between eutherian and metatherian mammal spermatozoa to examine the comparative resistance of sperm dna to oxidative damage in three eutherian species (mus musculus, homo sapiens, sus domesticus) and three metatherian species ... | 2011 | 21635811 |
| cytochrome p450 cyp3a in marsupials: cloning and characterisation of the second identified cyp3a subfamily member, isoform 3a78 from koala (phascolarctos cinereus). | cytochromes p450 (cyps) are critically important in the oxidative metabolism of a diverse array of xenobiotics and endogenous substrates. previously, we cloned and characterised the cyp2c, cyp4a, and cyp4b gene subfamilies from marsupials and demonstrated important species-differences in both activity and tissue expression of these cyp enzymes. recently, we isolated the eastern grey kangaroo cyp3a70. here we have cloned and characterised the second identified member of marsupial cyp3a gene subfa ... | 2011 | 21807118 |
| growth inhibition and chromosomal instability of cultured marsupial (opossum) cells after treatment with dna polymerase +¦ inhibitor. | the dna replication mechanism has been well established for eutherian mammals (placental mammals such as humans, mice, and cattle), but not, to date, for metatherian mammals (marsupials such as kangaroos, koalas, and opossums). in this study, we found that dehydroaltenusin, a selective inhibitor of mammalian (eutherian) dna polymerase +¦, clearly suppressed the growth of metatherian (opossum and rat kangaroo) cultured cells. in cultured opossum (ok) cells, dehydroaltenusin also suppressed the pr ... | 2011 | 21737927 |
| detection of a novel gammaherpesvirus in koalas (phascolarctos cinereus). | a novel gammaherpesvirus was detected in wild koalas (phascolarctos cinereus) captured at different locations during 2010. sequence analysis of the dna polymerase gene revealed that the virus was genetically distinct from all known gammaherpesviruses. this is the first herpesvirus to be definitively identified in the vombatiforme suborder (koalas and wombats). | 2011 | 21719855 |
| long-term surveillance and treatment of subclinical cryptococcosis and nasal colonization by cryptococcus neoformans and c. gattii species complex in captive koalas (phascolarctes cinereus). | cryptococcosis is an important systemic mycosis caused by members of the cryptococcus neoformans species complex. this disease is potentially fatal in various animals, including koalas. we describe the long-term surveillance and treatment of subclinical cryptococcosis and nasal colonization of koalas by cryptococcus neoformans and c. gattii. of the 15 animals investigated through the use of samples obtained by nasal swabs, antigen titer measurements, and pathologic examination, c. neoformans was ... | 2011 | 21859391 |
| relationships between the dynamics of iatrogenic dna damage and genomic design in mammalian spermatozoa from eleven species. | the dynamic onset of dna fragmentation in mammalian sperm populations varies widely in different species when the spermatozoa are incubated in vitro at body temperature for several hours, and recent studies have shown that the dynamic rate of dna fragmentation within a species has considerable predictive value in terms of fertility. the reasons for such variation are unclear, but here we show that differences in protamine sequence and identity could be partially responsible. sets of 10 normal se ... | 2011 | 21919111 |
| using quantitative polymerase chain reaction to correlate chlamydia pecorum infectious load with ocular, urinary and reproductive tract disease in the koala (phascolarctos cinereus). | complex interactions between chlamydia pecorum infection, the immune response and disease exist in the koala. we used quantitative polymerase chain reaction to investigate the relationship between c. pecorum infectious load and ocular and urogenital tract disease. chlamydia pecorum shedding was generally higher in animals with chronic, active disease than in animals with inactive disease. the absence of ocular disease was generally associated with low levels of shedding, but relatively high leve ... | 2011 | 21933169 |
| within-population diversity of koala chlamydophila pecorum at ompa vd1-vd3 and the orf663 hypothetical gene. | infection of koalas by chlamydophila pecorum is very common and causes significant morbidity, infertility and mortality. fundamental to management of the disease is an understanding of the importance of multi-serotype infection or pathogen virulence in pathogenesis; these may need consideration in plans involving koala movement, vaccination, or disease risk assessment. here we describe diversity of ompa vd1-3, and orf663 hypothetical gene tandem repeat regions, in a single population of koalas w ... | 2011 | 22118784 |
| vaccination of healthy and diseased koalas (phascolarctos cinereus) with a chlamydia pecorum multi-subunit vaccine: evaluation of immunity and pathology. | chlamydial infections represent a major threat to the long-term survival of the koala and a successful vaccine would provide a valuable management tool. vaccination however has the potential to enhance inflammatory disease in animals exposed to a natural infection prior to vaccination, a finding in early human and primate trials of whole cell vaccines to prevent trachoma. in the present study, we vaccinated both healthy koalas as well as clinically diseased koalas with a multi-subunit vaccine co ... | 2012 | 22230583 |
| spontaneous lymphoid neoplasia in the koala (phascolarctos cinereus). | thirteen cases of lymphoid neoplasia were examined in koalas from new south wales and queensland. haematological and biochemical data were collected from 2 cases while all were subjected to detailed necropsies. affected koalas were adults of both sexes and the disease had a variable time course. the 2 koalas examined haematologically were leukaemic. on a morphological basis, 5 cases were classified as multicentric, 7 cases as abdominal (but not necessarily with alimentary tract involvement) and ... | 2013 | 3597849 |
| chronic peritonitis and pancreatitis in a koala (phascolarctos cinereus). | the gross, light microscopic and ultrastructural appearance of a nodular peritonitis accompanied by pancreatic changes in a koala (phascolarctos cinereus) is described. its pathogenesis in relation to pancreatitis is discussed. | 2013 | 3734176 |
| multifarious activities of cellulose degrading bacteria from koala (phascolarctos cinereus) faeces. | cellulose degrading bacteria from koala faeces were isolated using caboxymethylcellulose-congo red agar, screened in vitro for different hydrolytic enzyme activities and phylogenetically characterized using molecular tools. bacillus sp. and pseudomonas sp. were the most prominent bacteria from koala faeces. the isolates demonstrated good xylanase, amylase, lipase, protease, tannase and lignin peroxidase activities apart from endoglucanase activity. furthermore many isolates grew in the presence ... | 2015 | 26290743 |
| host adaptation of chlamydia pecorum towards low virulence evident in co-evolution of the ompa, inca, and orf663 loci. | chlamydia (c.) pecorum, an obligate intracellular bacterium, may cause severe diseases in ruminants, swine and koalas, although asymptomatic infections are the norm. recently, we identified genetic polymorphisms in the ompa, inca and orf663 genes that potentially differentiate between high-virulence c. pecorum isolates from diseased animals and low-virulence isolates from asymptomatic animals. here, we expand these findings by including additional ruminant, swine, and koala strains. coding tande ... | 2014 | 25084532 |
| mhc class ii diversity of koala (phascolarctos cinereus) populations across their range. | major histocompatibility complex class ii (mhcii) genes code for proteins that bind and present antigenic peptides and trigger the adaptive immune response. we present a broad geographical study of mhcii da β1 (dab) and db β1 (dbb) variants of the koala (phascolarctos cinereus; n=191) from 12 populations across eastern australia, with a total of 13 dab and 7 dbb variants found. we identified greater mhcii variation and, possibly, additional gene copies in koala populations in the north (queensla ... | 2014 | 24690756 |
| the mammary gland-specific marsupial elp and eutherian cti share a common ancestral gene. | the marsupial early lactation protein (elp) gene is expressed in the mammary gland and the protein is secreted into milk during early lactation (phase 2a). mature elp shares approximately 55.4% similarity with the colostrum-specific bovine colostrum trypsin inhibitor (cti) protein. although elp and cti both have a single bovine pancreatic trypsin inhibitor (bpti)-kunitz domain and are secreted only during the early lactation phases, their evolutionary history is yet to be investigated. | 2012 | 22681678 |
| snp marker discovery in koala tlr genes. | toll-like receptors (tlrs) play a crucial role in the early defence against invading pathogens, yet our understanding of tlrs in marsupial immunity is limited. here, we describe the characterisation of nine tlrs from a koala immune tissue transcriptome and one tlr from a draft sequence of the koala genome and the subsequent development of an assay to study genetic diversity in these genes. we surveyed genetic diversity in 20 koalas from new south wales, australia and showed that one gene, tlr10 ... | 2015 | 25799012 |
| discovery of a novel retrovirus sequence in an australian native rodent (melomys burtoni): a putative link between gibbon ape leukemia virus and koala retrovirus. | gibbon ape leukaemia virus (galv) and koala retrovirus (korv) share a remarkably close sequence identity despite the fact that they occur in distantly related mammals on different continents. it has previously been suggested that infection of their respective hosts may have occurred as a result of a species jump from another, as yet unidentified vertebrate host. to investigate possible sources of these retroviruses in the australian context, dna samples were obtained from 42 vertebrate species a ... | 2014 | 25251014 |
| diet of dingoes and other wild dogs in peri-urban areas of north-eastern australia. | knowledge of the resource requirements of urban predators can improve our understanding of their ecology and assist town planners and wildlife management agencies in developing management approaches that alleviate human-wildlife conflicts. here we examine food and dietary items identified in scats of dingoes in peri-urban areas of north-eastern australia to better understand their resource requirements and the potential for dingoes to threaten locally fragmented populations of native fauna. our ... | 2016 | 26964762 |
| characterisation of the immune compounds in koala milk using a combined transcriptomic and proteomic approach. | production of milk is a key characteristic of mammals, but the features of lactation vary greatly between monotreme, marsupial and eutherian mammals. marsupials have a short gestation followed by a long lactation period, and milk constituents vary greatly across lactation. marsupials are born immunologically naïve and rely on their mother's milk for immunological protection. koalas (phascolarctos cinereus) are an iconic australian species that are increasingly threatened by disease. here we use ... | 2016 | 27713568 |
| the intestinal microbiota composition and weight development in children: the koala birth cohort study. | to investigate whether the intestinal microbiota composition in early infancy is associated with subsequent weight development in children. | 2015 | 25298274 |
| chlamydial infections in wildlife-conservation threats and/or reservoirs of 'spill-over' infections? | members of the order chlamydiales are biphasic intracellular pathogens known to cause disease in both humans and animals. as we learn more about the genetic diversity of this group of pathogens, evidence is growing that these bacteria infect a broader range of animal hosts than previously thought. over 400 host species are now documented globally with the majority of these being wild animals. given the impact of chlamydial infections on humans and domesticated animals, the identification of memb ... | 2016 | 27939160 |
| novel chlamydiales genotypes identified in ticks from australian wildlife. | members of the order chlamydiales are known for their potential as human and veterinary bacterial pathogens. despite this recognition, epidemiological factors such as routes of transmission are yet to be fully defined. ticks are well known vectors for many other infections with several reports recently describing the presence of bacteria in the order chlamydiales in these arthropods. australian wildlife are hosts to an extensive range of tick species. evidence is also growing that the marsupial ... | 2017 | 28122598 |
| treatment of chlamydia-associated ocular disease via a recombinant protein based vaccine in the koala (phascolarctos cinereus). | koalas (phascolarctos cinereus) are affected by debilitating chlamydial disease that can lead to blindness, infertility, and death. the causative agent is the intracellular bacterium chlamydia pecorum. while antibiotics can be used to treat koala chlamydial infection, they are often ineffective or cause severe dysbiosis to the animal's unique gut flora. recent work has progressed on the development of a protective vaccine for chlamydia in the koala. this study demonstrates that the use of a vacc ... | 2016 | 27707559 |
| pneumonia due to chlamydia pecorum in a koala (phascolarctos cinereus). | chlamydiosis is a common infectious disease of koalas (phascolarctos cinereus), but chlamydia spp. have not yet been demonstrated to cause pneumonia in these animals. a juvenile male koala died following an episode of respiratory disease. at necropsy examination, the lung tissue was consolidated. microscopical lesions in the lung included pyogranulomatous bronchopneumonia, proliferation of bronchiolar and alveolar epithelium and interstitial fibrosis. hyperplastic bronchiolar epithelial cells co ... | 2016 | 27567271 |
| penicillin g-induced chlamydial stress response in a porcine strain of chlamydia pecorum. | chlamydia pecorum causes asymptomatic infection and pathology in ruminants, pigs, and koalas. we characterized the antichlamydial effect of the beta lactam penicillin g on chlamydia pecorum strain 1710s (porcine abortion isolate). penicillin-exposed and mock-exposed infected host cells showed equivalent inclusions numbers. penicillin-exposed inclusions contained aberrant bacterial forms and exhibited reduced infectivity, while mock-exposed inclusions contained normal bacterial forms and exhibite ... | 2016 | 26997956 |
| chlamydia pecorum infection in free-ranging koalas ( phascolarctos cinereus ) on french island, victoria, australia. | we detected chlamydia pecorum in two koalas ( phascolarctos cinereus ) from a closed island population in victoria, australia, previously free of chlamydia infection. the ompa and multilocus sequence type were most closely related to published isolates of livestock rather than koala origin, suggesting potential cross-species transmission of c. pecorum . | 2016 | 26981690 |
| prevalence and pathologic features of chlamydia pecorum infections in south australian koalas (phascolarctos cinereus). | chlamydia pecorum infection is highly prevalent in many koala ( phascolarctos cinereus ) populations in the eastern states of australia, causing ocular and urogenital tract disease. in contrast, the current prevalence of chlamydiosis in south australian (sa) koalas is largely unknown, with few reports of clinical cases. we examined 65 sa rescued wild koalas at necropsy and collected ocular and urogenital swabs for the detection of c. pecorum by pcr. we detected c. pecorum in ocular or urogenital ... | 2016 | 26967132 |
| identification of unusual chlamydia pecorum genotypes in victorian koalas (phascolarctos cinereus) and clinical variables associated with infection. | chlamydia pecorum infection is a threat to the health of free-ranging koalas (phascolarctos cinereus) in australia. utilizing an extensive sample archive we determined the prevalence of c. pecorum in koalas within six regions of victoria, australia. the ompa genotypes of the detected c. pecorum were characterized to better understand the epidemiology of this pathogen in victorian koalas. despite many studies in northern australia (i.e. queensland and new south wales), prior chlamydia studies in ... | 2016 | 26932792 |
| characterisation of cd4 t cells in healthy and diseased koalas (phascolarctos cinereus) using cell-type-specific monoclonal antibodies. | the koala (phascolarctos cinereus) is an arboreal herbivorous marsupial that is an australian icon. koalas in many parts of australia are under multiple threats including habitat destruction, dog attacks, vehicular accidents, and infectious diseases such as chlamydia spp. and the koala retrovirus (korv), which may contribute to the incidence of lymphoma and leukaemia in this species. due to a lack of koala-specific immune reagents and assays there is currently no way to adequately analyse the im ... | 2016 | 26905635 |
| molecular characterisation of the chlamydia pecorum plasmid from porcine, ovine, bovine, and koala strains indicates plasmid-strain co-evolution. | background. highly stable, evolutionarily conserved, small, non-integrative plasmids are commonly found in members of the chlamydiaceae and, in some species, these plasmids have been strongly linked to virulence. to date, evidence for such a plasmid in chlamydia pecorum has been ambiguous. in a recent comparative genomic study of porcine, ovine, bovine, and koala c. pecorum isolates, we identified plasmids (pcpec) in a pig and three koala strains, respectively. screening of further porcine, ovin ... | 2016 | 26870613 |
| a prototype recombinant-protein based chlamydia pecorum vaccine results in reduced chlamydial burden and less clinical disease in free-ranging koalas (phascolarctos cinereus). | diseases associated with chlamydia pecorum infection are a major cause of decline in koala populations in australia. while koalas in care can generally be treated, a vaccine is considered the only option to effectively reduce the threat of infection and disease at the population level. in the current study, we vaccinated 30 free-ranging koalas with a prototype chlamydia pecorum vaccine consisting of a recombinant chlamydial momp adjuvanted with an immune stimulating complex. an additional cohort ... | 2016 | 26756624 |
| humoral immune responses in koalas (phascolarctos cinereus) either naturally infected with chlamydia pecorum or following administration of a recombinant chlamydial major outer membrane protein vaccine. | the development of a vaccine is a key strategy to combat the widespread and debilitating effects of chlamydial infection in koalas. one such vaccine in development uses recombinant chlamydial major outer membrane protein (rmomp) as an antigen and has shown promising results in several koala trials. previous chlamydial vaccine studies, primarily in the mouse model, suggest that both cell-mediated and antibody responses will be required for adequate protection. recently, the important protective r ... | 2016 | 26747718 |
| genetic diversity in the plasticity zone and the presence of the chlamydial plasmid differentiates chlamydia pecorum strains from pigs, sheep, cattle, and koalas. | chlamydia pecorum is a globally recognised pathogen of livestock and koalas. to date, comparative genomics of c. pecorum strains from sheep, cattle and koalas has revealed that only single nucleotide polymorphisms (snps) and a limited number of pseudogenes appear to contribute to the genetic diversity of this pathogen. no chlamydial plasmid has been detected in these strains despite its ubiquitous presence in almost all other chlamydial species. genomic analyses have not previously included c. p ... | 2015 | 26531162 |
| identification, characterisation and expression analysis of natural killer receptor genes in chlamydia pecorum infected koalas (phascolarctos cinereus). | koalas (phascolarctos cinereus), an iconic australian marsupial, are being heavily impacted by the spread of chlamydia pecorum, an obligate intracellular bacterial pathogen. koalas vary in their response to this pathogen, with some showing no symptoms, while others suffer severe symptoms leading to infertility, blindness or death. little is known about the pathology of this disease and the immune response against it in this host. studies have demonstrated that natural killer (nk) cells, key comp ... | 2015 | 26471184 |
| in vitro activity of chloramphenicol, florfenicol and enrofloxacin against chlamydia pecorum isolated from koalas (phascolarctos cinereus). | to determine the in vitro susceptibilities of koala isolates of chlamydia pecorum to enrofloxacin and chloramphenicol, which are frequently used to treat koalas with chlamydiosis, and florfenicol, a derivative of chloramphenicol. | 2015 | 26412342 |
| prevalence and clinical significance of herpesvirus infection in populations of australian marsupials. | herpesviruses have been reported in several marsupial species, but molecular classification has been limited to four herpesviruses in macropodids, a gammaherpesvirus in two antechinus species (antechinus flavipes and antechinus agilis), a gammaherpesvirus in a potoroid, the eastern bettong (bettongia gaimardi) and two gammaherpesviruses in koalas (phascolarctos cinereus). in this study we examined a range of australian marsupials for the presence of herpesviruses using molecular and serological ... | 2015 | 26222660 |
| culture-independent genome sequencing of clinical samples reveals an unexpected heterogeneity of infections by chlamydia pecorum. | chlamydia pecorum is an important global pathogen of livestock, and it is also a significant threat to the long-term survival of australia's koala populations. this study employed a culture-independent dna capture approach to sequence c. pecorum genomes directly from clinical swab samples collected from koalas with chlamydial disease as well as from sheep with arthritis and conjunctivitis. investigations into single-nucleotide polymorphisms within each of the swab samples revealed that a portion ... | 2015 | 25740768 |
| novel sequence types of chlamydia pecorum infect free-ranging alpine ibex (capra ibex) and red deer (cervus elaphus) in switzerland. | chlamydia pecorum, a recognized pathogen of domesticated ruminants and koalas (phascolarctos cinereus), has been recently reported in a broad range of other wildlife species including water buffalo (bubalus bubalis), ibex (capra ibex), chamois (rupicapra rupicapra), red deer (cervus elaphus), and birds. this identification raises questions as to whether cross-host transmission may be a factor in the epidemiology of infections in these species. to begin to address this question, we employed a c. ... | 2015 | 25647593 |
| orchitis and epididymitis in koalas (phascolarctos cinereus) infected with chlamydia pecorum. | although chlamydia causes disease of the urethra and prostate of male koalas, its impact on the testis and epididymis has not been examined. this study describes chronic-active and granulomatous orchitis and epididymitis with interstitial fibrosis associated with infection by chlamydia pecorum in 2 of 18 adult male koalas being euthanized at a koala hospital, 8 of which also had chlamydial prostatitis. by immunohistochemistry and transmission electron microscopy, chlamydial inclusions were demon ... | 2015 | 25637084 |
| the prevalence and clinical significance of chlamydia infection in island and mainland populations of victorian koalas (phascolarctos cinereus). | chlamydia infection is known to impact the health of koalas (phascolarctos cinereus) in new south wales (nsw) and queensland, but the clinical significance of chlamydia infections in victorian koalas is not well described. we examined the prevalence of chlamydia infection and assessed associated health parameters in two victorian koala populations known to be chlamydia positive. the same testing regimen was applied to a third victorian population in which chlamydia had not been detected. we exam ... | 2015 | 25588005 |
| comparison of subcutaneous versus intranasal immunization of male koalas (phascolarctos cinereus) for induction of mucosal and systemic immunity against chlamydia pecorum. | chlamydia pecorum infections are debilitating in the koala, contributing significantly to morbidity and mortality, with current antibiotic treatments having minimal success and adversely affecting gut microflora. this, combined with the sometimes-asymptomatic nature of the infection, suggests that an efficacious anti-chlamydial vaccine is required to control chlamydial infections in the koala. to date vaccination studies have focused primarily on female koalas, however, given the physiological d ... | 2015 | 25562793 |
| evaluation of the relationship between chlamydia pecorum sequence types and disease using a species-specific multi-locus sequence typing scheme (mlst). | chlamydia pecorum is globally associated with several ovine diseases including keratoconjunctivitis and polyarthritis. the exact relationship between the variety of c. pecorum strains reported and the diseases described in sheep remains unclear, challenging efforts to accurately diagnose and manage infected flocks. in the present study, we applied c. pecorum multi-locus sequence typing (mlst) to c. pecorum positive samples collected from sympatric flocks of australian sheep presenting with conju ... | 2014 | 25223647 |
| comparative genomics of koala, cattle and sheep strains of chlamydia pecorum. | chlamydia pecorum is an important pathogen of domesticated livestock including sheep, cattle and pigs. this pathogen is also a key factor in the decline of the koala in australia. we sequenced the genomes of three koala c. pecorum strains, isolated from the urogenital tracts and conjunctiva of diseased koalas. the genome of the c. pecorum vr629 (ipa) strain, isolated from a sheep with polyarthritis, was also sequenced. | 2014 | 25106440 |
| interleukin 17a is an immune marker for chlamydial disease severity and pathogenesis in the koala (phascolarctos cinereus). | the koala (phascolarctos cinereus) is an iconic australian marsupial species that is facing many threats to its survival. chlamydia pecorum infections are a significant contributor to this ongoing decline. a major limiting factor in our ability to manage and control chlamydial disease in koalas is a limited understanding of the koala's cell-mediated immune response to infections by this bacterial pathogen. to identify immunological markers associated with chlamydial infection and disease in koal ... | 2014 | 24915607 |
| chlamydiaceae and chlamydia-like organisms in the koala (phascolarctos cinereus)--organ distribution and histopathological findings. | chlamydial infections in koalas can cause life-threatening diseases leading to blindness and sterility. however, little is known about the systemic spread of chlamydiae in the inner organs of the koala, and data concerning related pathological organ lesions are limited. the aim of this study was to perform a thorough investigation of organs from 23 koalas and to correlate their histopathological lesions to molecular chlamydial detection. to reach this goal, 246 formalin-fixed and paraffin embedd ... | 2014 | 24888862 |
| expression profiles of the immune genes cd4, cd8β, ifnγ, il-4, il-6 and il-10 in mitogen-stimulated koala lymphocytes (phascolarctos cinereus) by qrt-pcr. | investigation of the immune response of the koala (phascolarctos cinereus) is needed urgently, but has been limited by scarcity of species-specific reagents and methods for this unique and divergent marsupial. infectious disease is an important threat to wild populations of koalas; the most widespread and important of these is chlamydial disease, caused by chlamydia pecorum and chlamydia pneumoniae. in addition, koala retrovirus (korv), which is of 100% prevalence in northern australia, has been ... | 2014 | 24688858 |
| conjunctivitis associated with chlamydia pecorum in three koalas (phascolarctos cinereus) in the mount lofty ranges, south australia. | chlamydiosis is a significant factor contributing to the decline of koala (phascolarctos cinereus) populations in australia but has not previously been reported in south australia. we describe conjunctivitis in three wild koalas from south australia, with chlamydia pecorum identified by quantitative pcr. | 2013 | 24502744 |
| vaccination of koalas with a recombinant chlamydia pecorum major outer membrane protein induces antibodies of different specificity compared to those following a natural live infection. | chlamydial infection in koalas is common across the east coast of australia and causes significant morbidity, infertility and mortality. an effective vaccine to prevent the adverse consequences of chlamydial infections in koalas (particularly blindness and infertility in females) would provide an important management tool to prevent further population decline of this species. an important step towards developing a vaccine in koalas is to understand the host immune response to chlamydial infectio ... | 2013 | 24086379 |
| genetic diversity of chlamydia pecorum strains in wild koala locations across australia and the implications for a recombinant c. pecorum major outer membrane protein based vaccine. | the long term survival of the koala (phascolarctos cinereus) is at risk due to a range of threatening processes. a major contributing factor is disease caused by infection with chlamydia pecorum, which has been detected in most mainland koala populations and is associated with ocular and genital tract infections. a critical aspect for the development of vaccines against koala chlamydial infections is a thorough understanding of the prevalence and strain diversity of c. pecorum infections across ... | 2013 | 24012135 |
| multilocus sequence analysis provides insights into molecular epidemiology of chlamydia pecorum infections in australian sheep, cattle, and koalas. | chlamydia pecorum is a significant pathogen of domestic livestock and wildlife. we have developed a c. pecorum-specific multilocus sequence analysis (mlsa) scheme to examine the genetic diversity of and relationships between australian sheep, cattle, and koala isolates. an mlsa of seven concatenated housekeeping gene fragments was performed using 35 isolates, including 18 livestock isolates (11 australian sheep, one australian cow, and six u.s. livestock isolates) and 17 australian koala isolate ... | 2013 | 23740730 |
| preliminary characterisation of tumor necrosis factor alpha and interleukin-10 responses to chlamydia pecorum infection in the koala (phascolarctos cinereus). | debilitating infectious diseases caused by chlamydia are major contributors to the decline of australia's iconic native marsupial species, the koala (phascolarctos cinereus). an understanding of koala chlamydial disease pathogenesis and the development of effective strategies to control infections continue to be hindered by an almost complete lack of species-specific immunological reagents. the cell-mediated immune response has been shown to play an influential role in the response to chlamydial ... | 2013 | 23527290 |
| recent advances in understanding the biology, epidemiology and control of chlamydial infections in koalas. | the koala (phascolarctos cinereus) is recognised as a threatened wildlife species in various parts of australia. a major contributing factor to the decline and long-term viability of affected populations is disease caused by the obligate intracellular bacteria, chlamydia. two chlamydial species infect the koala, chlamydia pecorum and chlamydia pneumoniae, and have been reported in nearly all mainland koala populations. chlamydial infections of koalas are associated with ocular infections leading ... | 2013 | 23523170 |
| antigenic specificity of a monovalent versus polyvalent momp based chlamydia pecorum vaccine in koalas (phascolarctos cinereus). | chlamydia continues to be a major pathogen of koalas. the bacterium is associated with ocular, respiratory and urogenital tract infections and a vaccine is considered the best option to limit the decline of mainland koala populations. over the last 20 years, efforts to develop a chlamydial vaccine in humans have focussed on the use of the chlamydial major outer membrane protein (momp). potential problems with the use of momp-based vaccines relate to the wide range of genetic diversity in its fou ... | 2013 | 23306372 |
| comparison of antigen detection and quantitative pcr in the detection of chlamydial infection in koalas (phascolarctos cinereus). | the gold standard method for detecting chlamydial infection in domestic and wild animals is pcr, but the technique is not suited to testing animals in the field when a rapid diagnosis is frequently required. the objective of this study was to compare the results of a commercially available enzyme immunoassay test for chlamydia against a quantitative chlamydia pecorum-specific pcr performed on swabs collected from the conjunctival sac, nasal cavity and urogenital sinuses of naturally infected koa ... | 2013 | 22944246 |
| human chlamydia pneumoniae isolates demonstrate ability to recover infectivity following penicillin treatment whereas animal isolates do not. | chlamydia pneumoniae strains have recently been demonstrated to have substantially different capacities to enter and recover from ifn-γ-induced persistence, depending on whether they are from human or animal host sources. here, we examined the ability of two human and two animal strains to enter and be rescued from penicillin-induced persistence. the ability to form inclusions after the addition of penicillin was much reduced in the two animal isolates (koala lpcoln, bandicoot b21) compared to t ... | 2015 | 25663156 |
| evidence for horizontal gene transfer between chlamydophila pneumoniae and chlamydia phage. | chlamydia-infecting bacteriophages, members of the microviridae family, specifically the gokushovirinae subfamily, are small (4.5-5 kb) single-stranded circles with 8-10 open-reading frames similar to e. coli phage ϕx174. using sequence information found in genbank, we examined related genes in chlamydophila pneumoniae and chlamydia-infecting bacteriophages. the 5 completely sequenced c. pneumoniae strains contain a gene orthologous to a phage gene annotated as the putative replication initiatio ... | 2017 | 26713222 |
| potential animal and environmental sources of q fever infection for humans in queensland. | q fever is a vaccine-preventable disease; despite this, high annual notification numbers are still recorded in australia. we have previously shown seroprevalence in queensland metropolitan regions is approaching that of rural areas. this study investigated the presence of nucleic acid from coxiella burnetii, the agent responsible for q fever, in a number of animal and environmental samples collected throughout queensland, to identify potential sources of human infection. samples were collected f ... | 2014 | 23663407 |
| atypical presentation of cryptococcus neoformans in a koala (phascolarctos cinereus): a magnetic resonance imaging and computed tomography study. | cryptococcosis is a worldwide and potentially fatal mycosis documented in wild and captive koalas ( phascolarctos cinereus ) caused by cryptococcus neoformans . though mainly a subclinical disease, when the nasal cavity is affected, epistaxis, mucopurulent nasal discharge, dyspnea, and facial distortion may occur. this report describes a case of cryptococcosis in a koala where unilateral exophthalmos was the only evident clinical sign and magnetic resonance imaging and computed tomography findin ... | 2017 | 28363053 |
| cryptococcus lacticolor sp. nov. and rhodotorula oligophaga sp. nov., novel yeasts isolated from the nasal smear microbiota of queensland koalas kept in japanese zoological parks. | a total of 515 yeast strains were isolated from the nasal smears of queensland koalas and their breeding environments in japanese zoological parks between 2005 and 2012. the most frequent species in the basidiomycetous yeast biota isolated from koala nasal passages was cryptococcus neoformans, followed by rhodotorula minuta. r. minuta was the most frequent species in the breeding environments, while c. neoformans was rare. seven strains representing two novel yeast species were identified. analy ... | 2013 | 23653119 |
| immunising with the transmembrane envelope proteins of different retroviruses including hiv-1: a comparative study. | the induction of neutralizing antibodies is a promising way to prevent retrovirus infections. neutralizing antibodies are mainly directed against the envelope proteins, which consist of two molecules, the surface envelope (su) protein and the transmembrane envelope (tm) protein. antibodies broadly neutralizing the human immunodeficiencvy virus-1 (hiv-1) and binding to the tm protein gp41 of the virus have been isolated from infected individuals. their epitopes are located in the membrane proxima ... | 2013 | 23249763 |
| koala retroviruses: characterization and impact on the life of koalas. | koala retroviruses (korv) have been isolated from wild and captive koalas in australia as well as from koala populations held in zoos in other countries. they are members of the genus gammaretrovirus, are most closely related to gibbon ape leukemia virus (galv), feline leukemia virus (felv) and porcine endogenous retrovirus (perv) and are likely the result of a relatively recent trans-species transmission from rodents or bats. the first korv to be isolated, korv-a, is widely distributed in the k ... | 2013 | 24148555 |
| a new look at the origins of gibbon ape leukemia virus. | is the origin of gibbon ape leukemia virus (galv) human after all? when galv was discovered and found to cause neoplastic disease in gibbons, it stimulated a great deal of research including investigations into the origins of this virus. a number of publications have suggested that the galv progenitor was a retrovirus present in one of several species of south east asian rodents that had close contact with captive gibbons. however, there are no published retroviral sequences from any south east ... | 2017 | 28220345 |
| transspecies transmission of gammaretroviruses and the origin of the gibbon ape leukaemia virus (galv) and the koala retrovirus (korv). | transspecies transmission of retroviruses is a frequent event, and the human immunodeficiency virus-1 (hiv-1) is a well-known example. the gibbon ape leukaemia virus (galv) and koala retrovirus (korv), two gammaretroviruses, are also the result of a transspecies transmission, however from a still unknown host. related retroviruses have been found in southeast asian mice although the sequence similarity was limited. viruses with a higher sequence homology were isolated from melomys burtoni, the a ... | 2016 | 27999419 |
| endogenous gibbon ape leukemia virus identified in a rodent (melomys burtoni subsp.) from wallacea (indonesia). | gibbon ape leukemia virus (galv) and koala retrovirus (korv) most likely originated from a cross-species transmission of an ancestral retrovirus into koalas and gibbons via one or more intermediate as-yet-unknown hosts. a virus highly similar to galv has been identified in an australian native rodent (melomys burtoni) after extensive screening of australian wildlife. galv-like viruses have also been discovered in several southeast asian species, although screening has not been extensive and viru ... | 2016 | 27384662 |
| episodic diversifying selection shaped the genomes of gibbon ape leukemia virus and related gammaretroviruses. | gibbon ape leukemia viruses (galvs) are part of a larger group of pathogenic gammaretroviruses present across phylogenetically diverse host species of australasian mammals. despite the biomedical utility of galvs as viral vectors and in cancer gene therapy, full genome sequences have not been determined for all of the five identified galv strains, nor has a comprehensive evolutionary analysis been performed. we therefore generated complete genomic sequences for each galv strain using hybridizati ... | 2015 | 26637454 |
| identification of diverse groups of endogenous gammaretroviruses in mega- and microbats. | a previous phylogenetic study suggested that mammalian gammaretroviruses may have originated in bats. here we report the discovery of rna transcripts from two putative endogenous gammaretroviruses in frugivorous (rousettus leschenaultii retrovirus, rlrv) and insectivorous (megaderma lyra retrovirus, mlrv) bat species. both genomes possess a large deletion in pol, indicating that they are defective retroviruses. phylogenetic analysis places rlrv and mlrv within the diversity of mammalian gammaret ... | 2012 | 22694899 |
| human and murine apobec3s restrict replication of koala retrovirus by different mechanisms. | koala retrovirus (korv) is an endogenous and exogenous retrovirus of koalas that may cause lymphoma. as for many other gammaretroviruses, the korv genome can potentially encode an alternate form of gag protein, glyco-gag. | 2015 | 26253512 |
| the potential impact of native australian trypanosome infections on the health of koalas (phascolarctos cinereus). | whole blood collected from koalas admitted to the australian zoo wildlife hospital (azwh), beerwah, qld, australia, during late 2006-2009 was tested using trypanosome species-specific 18s rdna pcrs designed to amplify dna from trypanosoma irwini, t. gilletti and t. copemani. clinical records for each koala sampled were reviewed and age, sex, blood packed cell volume (pcv), body condition, signs of illness, blood loss, trauma, chlamydiosis, bone marrow disease, koala aids and hospital admission o ... | 2011 | 21524321 |
| a comparative study of the corneal endothelium in vertebrates. | introduction: in vertebrates, a corneal endothelium is essential for the maintenance of corneal transparency in a variety of environments, including aerial, terrestrial and aquatic. knowledge of the surface structure of the corneal endothelium may assist our understanding of this unique tissue and its evolutionary development. except for humans and some mammals, there have been few studies of other vertebrates, particularly the unique australian species. methods: the field emission scanning elec ... | 2002 | 12482312 |
| the corneal surface of aquatic vertebrates: microstructures with optical and nutritional function? | the anterior surface of the mammalian cornea plays an important role in maintaining a smooth optical interface and consequently a sharp retinal image. the smooth surface is produced by a tear film, which adheres to a variety of microprojections, which increase the cell surface area, improve the absorbance of oxygen and nutrients and aid in the movement of metabolic products across the outer cell membrane. however, little is known of the structural adaptations and tear film support provided in ot ... | 2000 | 11079392 |
| interspecific variation of zona pellucida glycoconjugates in several species of marsupial. | the zona pellucida glycoconjugate content of several marsupial species was investigated using differential lectin histochemistry. ovaries from fat-tailed dunnarts, a southern brown bandicoot, grey short-tailed opossums, brushtail possums, ringtail possums, koalas and eastern grey kangaroos were fixed, embedded in paraffin wax, sectioned and stained with ten fluorescein isothiocyanate-conjugated lectins. sections were also incubated with either neuraminidase or saponified, respectively, before in ... | 2000 | 10864820 |
| ovulation-inducing factor in seminal plasma: a review. | ovulation in mammals involves pulsatile release of gnrh from the hypothalamus into the hypophyseal portal system with subsequent release of lh from the anterior pituitary into systemic circulation. elevated circulating concentrations of lh induce a cascade of events within the mature follicle, culminating in follicle rupture and evacuation. the broad classification of species as either spontaneous or induced ovulators is based on the type of stimulus responsible for eliciting gnrh release from t ... | 2013 | 23141951 |
| cytochrome p450 cyp3a in marsupials: cloning and identification of the first cyp3a subfamily member, isoform 3a70 from eastern gray kangaroo (macropus giganteus). | australian marsupials are unique fauna that have evolved and adapted to unique environments and thus it is likely that their detoxification systems differ considerably from those of well-studied eutherian mammals. knowledge of these processes in marsupials is therefore vital to understanding the consequences of exposure to xenobiotics. cytochromes p450 (cyps) are critically important in the oxidative metabolism of a diverse array of both xenobiotics and endogenous substrates. in this study we ha ... | 2012 | 22759518 |
| rate of digesta passage in the philippine flying lemur, cynocephalus volans. | the rate of digesta passage was measured in five captive philippine flying lemurs (cynocephalus volans). these animals were force fed capsules containing known quantities of either particulate or soluble markers. the volumes of the gastrointestinal tracts of three flying lemurs were determined based on the wet weight of the contents of each section of the gut. the mean rate of digesta passage was 14.37 +/- 3.31 h when determined using the particulate marker and 21.9 +/- 0.03 h when determined us ... | 1994 | 8089309 |
| in vitro hepatic microsomal metabolism of meloxicam in koalas (phascolarctos cinereus), brushtail possums (trichosurus vulpecula), ringtail possums (pseudocheirus peregrinus), rats (rattus norvegicus) and dogs (canis lupus familiaris). | quantitative and qualitative aspects of in vitro metabolism of the non-steroidal anti-inflammatory drug meloxicam, mediated via hepatic microsomes of specialized foliage (eucalyptus) eating marsupials (koalas and ringtail possums), a generalized foliage eating marsupial (brushtail possum), rats, and dogs, are described. using a substrate depletion method, intrinsic hepatic clearance (in vitro clint) was determined. significantly, rates of oxidative transformation of meloxicam, likely mediated vi ... | 2014 | 24345479 |
| unmanned aerial vehicles (uavs) and artificial intelligence revolutionizing wildlife monitoring and conservation. | surveying threatened and invasive species to obtain accurate population estimates is an important but challenging task that requires a considerable investment in time and resources. estimates using existing ground-based monitoring techniques, such as camera traps and surveys performed on foot, are known to be resource intensive, potentially inaccurate and imprecise, and difficult to validate. recent developments in unmanned aerial vehicles (uav), artificial intelligence and miniaturized thermal ... | 2016 | 26784196 |
| genomic characterization of the uncultured bacteroidales family s24-7 inhabiting the guts of homeothermic animals. | our view of host-associated microbiota remains incomplete due to the presence of as yet uncultured constituents. the bacteroidales family s24-7 is a prominent example of one of these groups. marker gene surveys indicate that members of this family are highly localized to the gastrointestinal tracts of homeothermic animals and are increasingly being recognized as a numerically predominant member of the gut microbiota; however, little is known about the nature of their interactions with the host. | 2016 | 27388460 |
| gastrointestinal torsions and intussusception in northern koalas (phascolarctos cinereus) at san diego zoo (1976-2012). | the recent classification as threatened status of the northern koala (phascolarctos cinereus) by the australian government highlights the importance of the conservation and health management of this iconic australian marsupial. this case series describes gastrointestinal torsion and intussusception in six northern koalas (three males, three females, 2-11 yr old) at the san diego zoo from 1976 to 2012. two koalas died shortly after presentation. diagnoses of ileocecal intussusception, resulting f ... | 2014 | 24712170 |