monitoring of wheat lactic acid bacteria from the field until the first step of dough fermentation. | the present work was carried out to retrieve the origin of lactic acid bacteria (lab) in sourdough. to this purpose, wheat lab were monitored from ear harvest until the first step of fermentation for sourdough development. the influence of the geographical area and variety on lab species/strain composition was also determined. the ears of four triticum durum varieties (duilio, iride, saragolla and simeto) were collected from several fields located within the palermo province (sicily, italy) and ... | 2017 | 27889157 |
characterization of fecal vancomycin-resistant enterococci with acquired and intrinsic resistance mechanisms in wild animals, spain. | the objectives were to evaluate the presence of vancomycin-resistant enterococci with acquired (vre-a) and intrinsic (vre-i) resistance mechanisms in fecal samples from different wild animals, and analyze their phenotypes and genotypes of antimicrobial resistance. a total of 348 cloacal/rectal samples from red-legged partridges (127), white storks (81), red kites (59), and wild boars (81) (june 2014/february 2015) were inoculated in slanetz-bartley agar supplemented with vancomycin (4 μg/ml). we ... | 2016 | 26194421 |
diversity of antibiotic resistance genes in enterococcus strains isolated from ready-to-eat meat products. | the objective of the study was to answer the question of whether the ready-to-eat meat products can pose indirect hazard for consumer health serving as reservoir of enterococcus strains harboring tetracyclines, aminoglycosides, and macrolides resistance genes. a total of 390 samples of ready-to-eat meat products were investigated. enterococcus strains were found in 74.1% of the samples. a total of 302 strains were classified as: enterococcus faecalis (48.7%), enterococcus faecium (39.7%), entero ... | 2016 | 27780292 |
identification of vancomycin-susceptible major clones of clinical enterococcus from algeria. | the main objectives of this study were to characterize clinical strains of enterococcus spp. isolated from algerian inpatients and outpatients, to investigate their susceptibility to antibiotics and to analyse their phylogenetic relatedness. a total of 85 non-duplicate enterococcus spp. isolates collected between 2010 and 2013 from various clinical samples, including urine, vaginal swab, pus, blood and semen, from algerian inpatients (n=62) and outpatients (n=23) were identified using matrix-ass ... | 2016 | 27530845 |
investigating bacterial population structure and dynamics in traditional koumiss from inner mongolia using single molecule real-time sequencing. | koumiss is considered as a complete dairy product high in nutrients and with medicinal properties. the bacterial communities involved in production of koumiss play a crucial role in the fermentation cycle. to reveal bacterial biodiversity in koumiss and the dynamics of succession in bacterial populations during fermentation, 22 samples were collected from 5 sampling sites and the full length of the 16s ribosomal rna genes sequenced using single molecule real-time sequencing technology. one hundr ... | 2016 | 27522429 |
detailed analyses of the bacterial populations in processed cocoa beans of different geographic origin, subject to varied fermentation conditions. | the quality of chocolate is influenced by several parameters, one of which is bacterial diversity during fermentation and drying; a crucial factor for the generation of the optimal cocoa flavor precursors. our understanding of the bacterial populations involved in chocolate fermentation can be improved by the use of high-throughput sequencing technologies (hts), combined with pcr amplification of the 16s rrna subunit. here, we have conducted a high-throughput assessment of bacterial diversity in ... | 2016 | 27458718 |
the generalist inside the specialist: gut bacterial communities of two insect species feeding on toxic plants are dominated by enterococcus sp. | some specialist insects feed on plants rich in secondary compounds, which pose a major selective pressure on both the phytophagous and the gut microbiota. however, microbial communities of toxic plant feeders are still poorly characterized. here, we show the bacterial communities of the gut of two specialized lepidoptera, hyles euphorbiae and brithys crini, which exclusively feed on latex-rich euphorbia sp. and alkaloid-rich pancratium maritimum, respectively. a metagenomic analysis based on hig ... | 2016 | 27446044 |
pitfalls in the identification of enterococcus species and the detection of vana and vanb genes. | the aims were to assess the performance of vitek 2 in identifying enterococcal species and the implementation of genexpert(®) vana/vanb pcr for the detection of vancomycin-resistant enterococci (vre). gram-positive cocci from clinical and environmental specimens (n = 431) suspicious of being enterococci by conventional methods were evaluated by vitek 2. this system identified 296 enterococcus faecium, 87 enterococcus faecalis, 10 enterococcus villorum, 9 enterococcus gallinarum, 9 enterococcus d ... | 2016 | 27367648 |
detection and dna quantification of enterococcus casseliflavus in a foal with septic meningitis. | case description a 3-month-old 180-kg (396-lb) hanoverian colt was examined because of fever, lethargy, inappetence, drooping of the left ear, and stiff neck posture. initial treatment included empirical antimicrobial treatment and nsaids. clinical findings initial findings were consistent with cns anomalies. endoscopy revealed hyperemia, ecchymosis, and some mucopurulent exudate in the right guttural pouch. hematologic findings were consistent with neutrophilic inflammation. on the third day of ... | 2016 | 27308888 |
glucosinolate and desulfo-glucosinolate metabolism by a selection of human gut bacteria. | glucosinolate (gsl) hydrolysis is mediated by the enzyme myrosinase which together with specifier proteins can give rise to isothiocyanates (itcs), thiocyanates, and nitriles (nits) in cruciferous plants. however, little is known about the metabolism of gsls by the human gut flora. the aim of the work was to investigate the metabolic fates of sinigrin (sng), glucotropaeolin (gtp), gluconasturtiin (gnt), and their corresponding desulfo-gsls (ds-gsls). three human gut bacterial strains, enterococc ... | 2016 | 27301252 |
clinical epidemiology of vancomycin-resistant enterococcus gallinarum and enterococcus casseliflavus bloodstream infections. | this study aimed to evaluate the clinical outcomes of vancomycin-resistant enterococcal bloodstream infections (vre bsi) caused by enterococcus gallinarum or enterococcus casseliflavus. variables associated with treatment failure were determined and treatment options were compared. this was a national retrospective study of hospitalised veterans affairs patients with non-faecium, non-faecalis vre bsi. the primary outcome was treatment failure, defined as a composite of: (i) 30-day all-cause mort ... | 2016 | 27274980 |
macrolide, glycopeptide resistance and virulence genes in enterococcus species isolates from dairy cattle. | the genus enterococcus is known to possess the capacity to acquire and disseminate antimicrobial resistant determinants alongside the ability to produce various virulence genes that enables it to establish infections. we assessed the prevalence and antibiogram profiles of enterococcus spp. in faecal samples of dairy cattle. faecal swab samples were collected from 400 dairy cattle from two commercial cattle farms in two rural communities in the eastern cape, south africa. confirmation of enteroco ... | 2016 | 27166215 |
host-derived probiotics enterococcus casseliflavus improves resistance against streptococcus iniae infection in rainbow trout (oncorhynchus mykiss) via immunomodulation. | the present study evaluated the benefits of dietary administration of host-derived candidate probiotics enterococcus casseliflavus in juvenile rainbow trout oncorhynchus mykiss. experimental diets were prepared by incorporating the microorganisms in the basal feed at 3 inclusion levels (i.e. 10(7) cfu g(-1) of feed [t1], 10(8) cfu g(-1) of feed [t2], 10(9) cfu g(-1) of feed [t3]). the probiotic feeds were administered for 8 weeks, with a group fed with the basal diet serving as control. the effe ... | 2016 | 26997202 |
enterococcus growth on eelgrass (zostera marina); implications for water quality. | enterococci are fecal indicator bacteria used to monitor fecal pollution of recreational waters. when enterococci levels exceed health standards, fecal pollution is assumed as the cause. enterococci growing on plants limit their usefulness as fecal indicator bacteria. here we examined enterococcal growth on eelgrass in mission bay, ca where enterococci levels have exceeded water quality thresholds. a total of 69 eelgrass samples were collected from six sites, shaken to remove enterococci attache ... | 2016 | 26976844 |
emergence of antimicrobial resistance and virulence factors among the unusual species of enterococci, from north india. | several enterococcal species are increasingly being reported from clinical infections, besides the major species. | 2017 | 26960635 |
identification and prevalence of tetracycline resistance in enterococci isolated from poultry in ilishan, ogun state, nigeria. | tetracycline is one of the most frequently used antibiotics in nigeria both for human and animal infections because of its cheapness and ready availability. the use of tetracycline in animal husbandry could lead to horizontal transfer of tet genes from poultry to human through the gut microbiota, especially enterococci. therefore, this study is designed to identify different enterococcal species from poultry feces in selected farms in ilishan, ogun state, nigeria, determine the prevalence of tet ... | 2017 | 26957873 |
aerobic degradation of bde-209 by enterococcus casseliflavus: isolation, identification and cell changes during degradation process. | decabromodiphenyl ether (bde-209) is one of the most commonly used brominated flame retardants that have contaminated the environment worldwide. microbial bioremediation has been considered as an effective technique to remove these sorts of persistent organic pollutants. enterococcus casseliflavus, a gram-positive bacterium capable of aerobically transforming bde-209, was isolated by our team from sediments in guiyu, an e-waste dismantling area in guangdong province, china. to promote microbial ... | 2016 | 26852209 |
exogenous endophthalmitis caused by enterococcus casseliflavus: a case report and discussion regarding treatment of intraocular infection with vancomycin-resistant enterococci. | endophthalmitis caused by enterococci is rare, and cases involving vancomycin-resistant enterococci are even more so. due to the poor bioavailability of many antibiotics in the vitreous chamber, special considerations are required when choosing antibiotics to treat these infections. the authors report the first case of exogenous endophthalmitis caused by enterococcus casseliflavus via the unique mechanism of high-velocity water stream trauma from a toy water gun. a previously healthy four-year o ... | 2017 | 26744592 |
sensitivity of antibiotic resistant and antibiotic susceptible escherichia coli, enterococcus and staphylococcus strains against ozone. | tolerance of antibiotic susceptible and antibiotic resistant escherichia coli, enterococcus and staphylococcus strains from clinical and wastewater samples against ozone was tested to investigate if ozone, a strong oxidant applied for advanced wastewater treatment, will affect the release of antibiotic resistant bacteria into the aquatic environment. for this purpose, the resistance pattern against antibiotics of the mentioned isolates and their survival after exposure to 4 mg/l ozone was determ ... | 2015 | 26608763 |
effect of in-feed administration and withdrawal of tylosin phosphate on antibiotic resistance in enterococci isolated from feedlot steers. | tylosin phosphate is a macrolide commonly administered to cattle in north america for the control of liver abscesses. this study investigated the effect of in-feed administration of tylosin phosphate to cattle at subtherapeutic levels and its subsequent withdrawal on macrolide resistance using enterococci as an indicator bacterium. fecal samples were collected from steers that received no antibiotics and steers administered tylosin phosphate (11 ppm) in-feed for 197 days and withdrawn 28 days be ... | 2015 | 26074889 |
antimicrobial resistance profile of enterococcus species isolated from intestinal tracts of hospitalized patients in jimma, ethiopia. | multi-drug-resistant enterococci colonizing the intestinal tract of hospitalized patients are the major source of infection as well as nosocomial spread. despite worldwide increasing rate of multidrug resistant enterococci colonization and infection among hospitalized patients, there is scarcity of data from resource limited setting. the present study aimed at determining the antimicrobial resistance profile of enterococcus species from intestinal tracts of hospitalized patients in jimma, ethiop ... | 2015 | 26036911 |
diversity of enterococcal species and characterization of high-level aminoglycoside resistant enterococci of samples of wastewater and surface water in tunisia. | one hundred-fourteen samples of wastewater (n=64) and surface-water (n=50) were inoculated in slanetz-bartley agar plates supplemented or not with gentamicin (sb-gen and sb plates, respectively) for enterococci recovery. enterococci were obtained from 75% of tested samples in sb media (72% in wastewater; 78% in surface-water), and 85 enterococcal isolates (one/positive-sample) were obtained. enterococcus faecium was the most prevalent species (63.5%), followed by enterococcus faecalis (20%), ent ... | 2015 | 26026404 |
prevalence, diversity and characterization of enterococci from three coraciiform birds. | coraciiform birds hoopoe (upupa epops), common kingfisher (alcedo atthis) and european roller (coracius garrulus) were examined for enterococci in their cloacae and uropygial glands. the enterococcal isolates were identified at the species level using several genomic and proteomic methods, screened for antibiotic susceptibility and genotyped by pulsed-field gel electrophoresis (pfge). clonality of isolates from the common kingfisher was also assessed by multi-locus sequence typing (mlst). using ... | 2015 | 25772302 |
enterococcus casseliflavus and enterococcus gallinarum as causative agents of spontaneous bacterial peritonitis. | infection by multidrug resistant bacteria is arousing as a relevant issue among hospitalized subjects and is of particular interest in patients with cirrhosis given the frequent use of broad spectrum antibiotics and their altered immune response. we report the first case report of spontaneous bacterial peritonitis (sbp) caused by enterococcus casseliflavus and the sixth case of sbp caused by enterococcus gallinarum. | 2015 | 25671838 |
enterococcus casseliflavus endophthalmitis due to metallic intraocular foreign body. | | 2015 | 25657040 |
identification of enterococcus faecium and enterococcus faecalis as vanc-type vancomycin-resistant enterococci (vre) from sewage and river water in the provincial city of miyazaki, japan. | as a first step for assessing the risk to human health posed by vancomycin-resistant enterococci (vre) in the aquatic environment, we screened sewage and urban river water samples from miyazaki, japan for vre. because vancomycin-resistant organisms are not as prevalent in sewage and river water as vancomycin-susceptible organisms, the samples were screened by minimum inhibitory concentration test using the vancomycin-supplemented membrane-enterococcus indoxyl-β-d-glucoside (mei) agar. the isolat ... | 2015 | 25438128 |
phenotypic and genotypic identification of lactic acid bacteria isolated from traditional pickles of the çubuk region in turkey. | a total of 152 lactic acid bacteria (lab) were isolated from pickles produced in the ankara-çubuk region. these isolates were clustered into eight groups on the basis of their phenotypic characteristics including cell morphology, co2 production from glucose, growth at 10 and 45 °c, growth in 6.5 % nacl, and growth at ph 9.6. api 50 ch carbohydrate fermentation test, 16s ribosomal rna (rrna) sequence analysis, and sodium dodecyl sulfate-acrylamide gel electrophoresis (sds-page) whole-cell protein ... | 2015 | 25404550 |
the combinations lysobacter enzymogenes subsp. enzymogenes christensen and cook 1978, l. enzymogenes subsp. cookii christensen 1978 and streptococcus casseliflavus (mundt and graham 1968) vaughan et al. 1979 were in accordance with the international code of nomenclature of bacteria at the time of publication in the international journal of systematic bacteriology, but are not to be considered to be included on the approved lists of bacterial names. opinion 95: judicial commission of the international committee on systematics of prokaryotes. | the judicial commission affirms that, according to information presented to it, the combination lysobacter enzymogenes subsp. enzymogenes christensen and cook 1978, the combination lysobacter enzymogenes subsp. cookii christensen 1978 and the combination streptococcus casseliflavus (mundt and graham 1968) vaughan et al. 1979 were in accordance with the wording of the 1975 and 1992 revisions of the international code of nomenclature of bacteria but they are not to be considered to be included on ... | 2014 | 25386007 |
enterococcus--virulence and susceptibility to photodynamic therapy of clinical isolates from lower silesia, poland. | a collection of 400 enterococcal isolates from clinical samples of hospitalized patients were studied for their virulence traits according to the isolation site. formation of biofilm and production of dnase, hemolysin, lipase, and gelatinase were characterized. biofilms of selected strains were examined for their susceptibility to antimicrobial photodynamic therapy (apdt). | 2014 | 25290581 |
antimicrobial susceptibility of enterococcal species isolated from antibiotic-treated dogs and cats. | in this study, we examined the antimicrobial susceptibility of the enterococci isolated from dogs and cats in japan during 2011-2012. fecal samples were collected from 84 dogs and 16 cats that underwent antibiotic treatment. enterococci were detected in 70 of 84 dogs (83.3%) and 7 of 16 cats (43.8%). the most prevalent enterococcus species was enterococcus faecalis (64.9%); enterococcus faecium and enterococcus durans were also isolated from 14 of 77 (18.2%) and 5 of 77 (6.5%) of these animals, ... | 2014 | 24976587 |
first report of multiresistance gene cfr in enterococcus species casseliflavus and gallinarum of swine origin. | the aim of this study was to investigate the presence and genetic environment of the multiresistance gene cfr in enterococcus species of swine origin. twenty-five cfr-carrying enterococcus isolates were collected from swine in beijing, guangzhou, and shandong, china. the isolates consist of 24 enterococcus casseliflavus and one enterococcus gallinarum isolate, and exhibited six smai pfge patterns. the cfr gene was located on plasmids in all isolates except e. casseliflavus en83, in which cfr was ... | 2014 | 24690374 |
vana-containing e. faecium isolates of clonal complex cc17 in clinical and environmental samples in a tunisian hospital. | twenty-eight vancomycin (va)-resistant enterococci isolated from different patients (n = 16) and also from the environment (n = 12) were recovered in a tunisian military hospital during 2012-2013. the mechanisms of resistance to va and to other antibiotics as well as the presence of esp and hyl virulence genes were determined in these isolates by pcr, being their clonal relationship analyzed by pulsed-field gel electrophoresis (pfge). va resistance mechanisms detected were as follows (species-pa ... | 2014 | 24565851 |
prevalence of enterococcus species and their virulence genes in fresh water prior to and after storm events. | enterococcus spp. isolates (n = 286) collected from six surface water bodies in subtropical brisbane, australia, prior to and after storm events, were identified to species level and tested for the presence of seven clinically important virulence genes (vgs). enterococcus faecalis (48%), enterococcus faecium (14%), enterococcus mundtii (13%), and enterococcus casseliflavus (13%) were frequently detected at all sites. the frequency of e. faecium occurrence increased from 6% in the dry period to 1 ... | 2014 | 24494806 |
animal rennets as sources of dairy lactic acid bacteria. | the microbial composition of artisan and industrial animal rennet pastes was studied by using both culture-dependent and -independent approaches. pyrosequencing targeting the 16s rrna gene allowed to identify 361 operational taxonomic units (otus) to the genus/species level. among lactic acid bacteria (lab), streptococcus thermophilus and some lactobacilli, mainly lactobacillus crispatus and lactobacillus reuteri, were the most abundant species, with differences among the samples. twelve groups ... | 2014 | 24441167 |
isolation of alkaliphilic bacteria for production of high optically pure l-(+)-lactic acid. | lactic acid bacteria that grow under alkaline conditions (ph 10) were isolated from various sources in okinawa (japan). these alkali-tolerant and alkaliphilic bacteria were classified as follows: microbacterium sp. (1 strain), enterococcus spp. (9 strains), alkalibacterium spp. (3 strains), exiguobacterium spp. (5 strains), oceanobacillus spp. (3 strains) and bacillus spp. (7 strains) by 16s rrna gene sequencing. by fermentation, many strains were able to convert glucose into mainly l-(+)-lactic ... | 2014 | 25742979 |
[characteristics and functional protein analysis of an effective decabromodiphenyl ether-degrading strain]. | an effective decabromodiphenyl ether (bde-209) degrading strain was isolated and identified as enterococcus casseliflavus based on the 16s rrna gene sequence analysis. the optimal conditions for strain growth were ph 7 and culture time of 48 h, respectively. e. casseliflavus has a good ability to degrade bde-209. the biodegradation rate of 1 mg.l-1 bde-209 by 1 g.l-1 e. casseliflavus reached the highest of 56. 7% after 4 days degradation with 5 mg.l-1 glucose as the additional carbon source. dur ... | 2013 | 24364338 |
antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk. | recent studies have shown that mammalian milk represents a continuous supply of commensal bacteria, including enterococci. the objectives of this study were to evaluate the presence of enterococci in milk of different species and to screen them for several genetic and phenotypic traits of clinical significance among enterococci. | 2013 | 24325647 |
application of ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry to determine the metabolites of orientin produced by human intestinal bacteria. | an ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (uplc-q-tof/ms) with metabolynx™ software combined with mass defect filtering (mdf) method were provided for orientin metabolism study. the chromatographic separation was performed on a 1.7μm particle size syncronis c18 column using gradient elution system. the components were identified and confirmed according to the mass spectrometric fragmentation mechanisms, ms/ms fragment ions and relevant literature by m ... | 2014 | 24316522 |
presence of the vancomycin resistance gene cluster vanc1, vanxyc, and vant in enterococcus casseliflavus. | the three chromosomally located clustered genes vanc1, vanxyc, and vant confer intrinsic resistance to vancomycin and are used for species identification of enterococcus gallinarum. in this study, 28 strains belonging to the e. gallinarum/casseliflavus group isolated from cloacal swabs from laying hens were screened for the presence of vanc1. as confirmed by species-specific multiplex pcr, 11 vanc1-positive strains were identified as e. gallinarum. surprisingly, one yellow pigmented strain, veri ... | 2014 | 24266667 |
the metabolism of methylsulfinylalkyl- and methylthioalkyl-glucosinolates by a selection of human gut bacteria. | certain myrosinase-positive human gut bacteria can metabolize glucosinolates (gsls) to produce isothiocyanates (itc) as chemopreventive agents. we investigated glucoerucin, glucoiberin, and glucoraphanin (present in broccoli) metabolism by human gut strains. | 2014 | 24170324 |
diversity, distribution and antibiotic resistance of enterococcus spp. recovered from tomatoes, leaves, water and soil on u.s. mid-atlantic farms. | antibiotic-resistant enterococci are important opportunistic pathogens and have been recovered from retail tomatoes. however, it is unclear where and how tomatoes are contaminated along the farm-to-fork continuum. specifically, the degree of pre-harvest contamination with enterococci is unknown. we evaluated the prevalence, diversity and antimicrobial susceptibilities of enterococci collected from tomato farms in the mid-atlantic united states. tomatoes, leaves, groundwater, pond water, irrigati ... | 2013 | 24010630 |
cloning and heterologous expression of plne, -f, -j and -k genes derived from soil metagenome and purification of active plantaricin peptides. | plantaricin gene-specific primers were used to obtain plne, -f, -j and -k structural gene amplicons from soil metagenome. these amplicons were cloned and expressed in pet32a (+) vector in escherichia coli bl21 (de3). plne, -f, -j and -k peptides were expressed as his-tagged-fusion proteins and were separated by ni(2+) -chelating affinity chromatography. the peptides were released from the fusion by enterokinase cleavage and separated from the carrier thioredoxin. the cleaved peptides were furthe ... | 2014 | 23884205 |
detection of vanc1 gene transcription in vancomycin-susceptible enterococcus faecalis. | here we report the presence and expression levels of the vanc1 and vanc(2/3) genes in vancomycin-susceptible strains of enterococcus faecalis. the vanc1 and vanc(2/3) genes were located in the plasmid dna and on the chromosome, respectively. specific mrna of the vanc1 gene was detected in one of these strains. additionally, analysis of the vanc gene sequences showed that these genes are related to the vanc genes of enterococcus gallinarum and enterococcus casseliflavus. the presence of vanc gene ... | 2013 | 23828012 |
antibiotics and heavy metals resistance and other biological characters in enterococci isolated from surface water of monte cotugno lake (italy). | considering the limited knowledge about the biological characters in enterococci isolated from surface waters, we investigated antibiotic and heavy-metal resistance, bacteriocin production, and some important virulence traits of 165 enterococci collected in water samples from monte cotugno lake, the largest artificial basin built with earth in europe. the species distribution of isolates was as follows: enterococcus faecium (80%), enterococcus faecalis (12.7%), enterococcus casseliflavus (3%), e ... | 2013 | 23485245 |
assessment of safe enterococci as bioprotective cultures in low-acid fermented sausages combined with high hydrostatic pressure. | three bacteriocinogenic, non-aminogenic and non-virulent enterococcus strains (enterococcus faecium ctc8005, enterococcus devriesei ctc8006 and enterococcus casseliflavus ctc8003) were used as starter cultures in low-acid fermented sausages to assess their competitiveness and their bioprotective potential against listeria monocytogenes and staphylococcus aureus. the inoculated strains were successfully monitored by rapd-pcr. all the strains were able to grow, survive and dominate the endogenous ... | 2013 | 23200647 |
isolation and characterization of lactic acid bacteria from pobuzihi (fermented cummingcordia), a traditional fermented food in taiwan. | lactobacillus pobuzihii is a novel species which has been previously found in pobuzihi (fermented cummingcordia), a traditional fermented food in taiwan. however, the lactic acid bacteria (lab) microflora in pobuzihi has not been studied in detail. in this study, lab from pobuzihi were isolated, identified, and characterized. a total of 196 lab were isolated; 79 cultures were isolated from the sample collected from a manufacturing factory, 38 from pobuzihi samples collected from 4 different mark ... | 2013 | 23011950 |
evaluation of species-specific pcr, bruker ms, vitek ms and the vitek 2 system for the identification of clinical enterococcus isolates. | the purpose of this investigation was to compare the performance of species-specific polymerase chain reaction (pcr), matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof ms) and phenotypic identification systems for the identification of enterococcus species. a total of 132 clinical isolates were investigated by the following: (1) a multiplex real-time pcr assay targeting ddl enterococcus faecium, ddl enterococcus faecalis, vanc1 and vanc2/c3 genes, and a high ... | 2012 | 22706514 |
study of vancomycin resistance in faecal enterococci from healthy humans and dogs in spain a decade after the avoparcin ban in europe. | one hundred and 26 faecal samples from healthy dogs (2009) and 157 faecal samples from healthy humans (2007) from la rioja region (spain) were tested to know the carriage of vancomycin-resistant enterococci (vre). vre with intrinsic resistance (vanc) were found in 12% of healthy dogs and humans (29 enterococcus gallinarum and four enterococcus casseliflavus). nevertheless, vre with acquired mechanism of resistance were not detected among these samples. four enterococcus faecalis isolates with va ... | 2013 | 22646722 |
presence of erythromycin and tetracycline resistance genes in lactic acid bacteria from fermented foods of indian origin. | lactic acid bacteria (lab) resistant to erythromycin were isolated from different food samples on selective media. the isolates were identified as enterococcus durans, enterococcus faecium, enterococcus lactis, enterococcus casseliflavus, lactobacillus salivarius, lactobacillus reuteri, lactobacillus plantarum, lactobacillus fermentum, pediococcus pentosaceus and leuconostoc mesenteroides. of the total 60 isolates, 88 % harbored the ermb gene. the efflux gene msra was identified in e. faecium, e ... | 2012 | 22644346 |
persistence and growth of the fecal indicator bacteria enterococci in detritus and natural estuarine plankton communities. | enterococci are used to evaluate recreational-water quality and health risks in marine environments. in addition to their occurrence in feces of warm blooded animals, they are also common epiphytes. we investigated the contribution of plankton- or particle-associated enterococci in estuarine and coastal water. seven water and size-fractionated plankton samples were collected monthly between april 2008 and january 2009 in the tidal reaches of the skidaway river (georgia, usa). each size fraction, ... | 2012 | 22327586 |
risk factors for vancomycin-resistant enterococci colonisation in critically ill patients. | vancomycin-resistant enterococci (vre) are important hospital pathogens and have become increasingly common in patients admitted to the intensive care unit (icu). to determine the incidence and the risk factors associated with vre colonisation among icu patients, active surveillance cultures for vre faecal carriages were carried out in patients admitted to the icu of the university hospital of uberlândia, minas gerais, brazil. risk factors were assessed using a case-control study. seventy-seven ... | 2012 | 22310536 |
isolation and characterisation of lactic acid bacteria from jiang-gua (fermented cucumbers), a traditional fermented food in taiwan. | jiang-gua (fermented cucumbers) is a popular traditional fermented food in taiwan. the microflora of lactic acid bacteria (lab) in jiang-gua have not been investigated in detail. in this study, lab from jiang-gua were isolated, characterised and identified. | 2012 | 22271629 |
vancomycin-resistant enterococci with vana gene in treated municipal wastewater and their association with human hospital strains. | vancomycin-resistant enterococci (vre) are pathogens of increasing medical importance. in brno, czech republic, we collected 37 samples from the effluent of a wastewater treatment plant (wwtp), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. moreover, we collected 284 gull cloacal swabs from the colony situated 35km downstream the wwtp. samples were cultured selectively. enterococci were identified using maldi-tof ms, phenotypically tested for ... | 2017 | 28763660 |
successful management of post-traumatic vancomycin-resistant enterococcus endophthalmitis. | to highlight good visual outcome with intravitreal amikacin administered 18 days following trauma-inducing vancomycin resistant enterococcal endophthalmitis treated initially with vitrectomy and oral linezolid. | 2017 | 28848938 |
enterococcus casseliflavus septicaemia associated with hepatobiliary infection in a 75-year-old man. | enterococcus casseliflavus is a rare non-faecium, non-faecalis, vancomycin-resistant enterococcus (vre) that is responsible for up to 2% of all enterococcal infections. septicaemia with this bacterium is usually seen in older patients with multiple comorbidities who have had several previous hospitalisations. septicaemia with e. casseliflavus portends a poorer prognosis, and treatment usually involves administration of antienterococcal beta-lactams or anti-vre medications such as linezolid or da ... | 2017 | 28500123 |
environmental growth of enterococci and escherichia coli in feedlot catch basins and a constructed wetland in the absence of fecal input. | population structures of fecal indicator bacteria (fib) isolated from catch basins, a constructed wetland, and feces from a beef cattle feedlot were compared over a two-year period. enterococcus hirae accounted for 92% of the fecal isolates, whereas secondary environments were characterized by greater relative abundance of environmentally adapted species including enterococcus casseliflavus. while enterococci densities in the catch basins and wetland were similar under wet and drought conditions ... | 2017 | 28430425 |
draft genome sequence of enterococcus casseliflavus pavet15 obtained from the oviduct infection of the cattle tick (rhipicephalus microplus) in jiutepec, morelos, mexico. | enterococcus spp. are gram-positive lactic acid-producing bacteria found in the intestinal tracts of animals, like mammals, birds, and arthropods. enterococcus spp. may cause oportunistic infections in vertebrate and invertebrate hosts. we report here the draft genome sequence of enterococcus casseliflavus pavet15 containing 3,722,480 bp, with 80 contigs, an n50 of 179,476 bp, and 41.93% g+c content. | 2017 | 28428300 |
bloodstream infections caused by enterococcus spp: a 10-year retrospective analysis at a tertiary hospital in china. | in order to discover the risk factors for 30-day mortality in bloodstream infections (bsi) caused by enterococcus spp. strains, we explored the clinical and therapeutic profile of patients with enterococcus spp. bsi and the characteristics of this condition. a total of 64 patients with bsi caused by enterococcus spp. who were treated in our hospital between 2006 and 2015 were included in the study. the clinical features of patients, microbiology, and 30-day mortality were collected from the elec ... | 2017 | 28397040 |