| brucella melitensis b115-based complement fixation test to detect antibodies induced by brucella rough strains. | to assess the efficiency of a brucella melitensis b115 rough strain, naturally devoid of anticomplementary activity, used as antigen in a complement fixation test (cft) to detect antibodies induced by brucella strains with rough phenotype, such as brucella abortus rb51, brucella ovis and brucella canis. | 2008 | 18355234 |
| bacterial and protozoal causes of pregnancy loss in the bitch and queen. | several bacterial species have been implicated in canine and feline pregnancy loss. brucella canis is one of the more important bacterial infectious agents that cause pregnancy loss in the bitch. brucella has been documented in the queen but in general infectious abortion from bacteria and protozoal agents is uncommon in the species. protozoal causes of pregnancy loss in the bitch and queen are less common than in other species. etiology, clinical signs, diagnosis and treatment of bacterial and ... | 2008 | 18495236 |
| comparison of agar gel immunodiffusion test, rapid slide agglutination test, microbiological culture and pcr for the diagnosis of canine brucellosis. | the performance of the rapid slide agglutination test, with and without 2-mercaptoethanol (rsat and 2me-rsat) and agar gel immunodiffusion test (agid) was evaluated for the diagnosis of brucellosis in naturally infected dogs. the microbiological culture, pcr and clinical parameters were used as reference. a total of 167 dogs were clinically examined and tested by blood culture, culture of semen/vaginal swab and pcr in blood and semen/vaginal swab. according to the results observed the 167 dogs w ... | 2009 | 18656213 |
| novel identification and differentiation of brucella melitensis, b. abortus, b. suis, b. ovis, b. canis, and b. neotomae suitable for both conventional and real-time pcr systems. | we describe the development of a novel pcr assay for the rapid detection of members of the brucella genus, and the differentiation between six recognized brucella species. the assay has proven to be highly specific with the additional advantage of being suitable for use with both conventional and real-time pcr. | 2008 | 18675856 |
| evaluation of a microplate agglutination test (mat) for serological diagnosis of canine brucellosis. | a microplate agglutination test (mat) was compared with the tube agglutinin test (tat), a standard test for the diagnosis of brucella canis, in terms of the sensitivity and specificity. the results showed that mat was more sensitive, simpler to perform and easier to read the results than tat. on top of that the mat allows us to handle a larger number of samples at once. using this method we conducted sero-surveillance of the prevalence of b. canis in dogs kept in an animal shelter located in kan ... | 2008 | 18685243 |
| [canine brucellosis in dogs in the city of buenos aires]. | canine brucellosis, caused by brucella canis, provokes epidydimitis, testicular atrophy and sterility in male dogs, while in females the major symptom is miscarriage. transmission to humans may be through contact with semen, urine and/or aborted fetuses of infected animals. our study, observational and cross-sectional, focused on dogs in lower class neighborhoods and slums with a high rate of unmet basic needs (ubn) in 8 areas of the city of buenos aires. we studied 219 dogs: 184 females and 35 ... | 2008 | 18786885 |
| canine brucellosis in a saskatchewan kennel. | canine brucellosis is rare in canada. this report describes an outbreak of brucella canis infection within a kennel, emphasizing diagnostic and pathologic findings. gender differences are described. the progestational, nongravid uterus, female spleen, and prostate gland are consistent sites of bacterial isolation. | 2008 | 18827848 |
| seroprevalence of canine herpesvirus-1 and brucella canis in finnish breeding kennels with and without reproductive problems. | we compared the serological status of brucella canis and canine herpesvirus-1 (chv-1) in finnish breeding kennels with and without reproductive problems. dogs from kennels with reproductive problems had significantly higher chv-1 titres than dogs from kennels having no reproductive problems (p < 0.001). in dogs from kennels with reproductive problems 100% (32/32) had positive titres, whereas in dogs from kennels without reproductive problems 65% (22/34) had positive titres. the median titre for ... | 2009 | 18992103 |
| enzyme-linked immunosorbent assay for screening of canine brucellosis using recombinant cu-zn superoxide dismutase. | brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. the diagnosis of canine brucellosis is based on bacteriological examination and serological methods including agglutination and gel diffusion tests. in this study, recombinant b. canis cu-zn superoxide dismutase (sod) was used as an antigen for the enzyme-linked immunosorbent assay (elisa). the recombinant sod showed a specific reaction with serum infected with b. canis in western blotting and eli ... | 2008 | 19122412 |
| analysis of ten brucella genomes reveals evidence for horizontal gene transfer despite a preferred intracellular lifestyle. | the facultative intracellular bacterial pathogen brucella infects a wide range of warm-blooded land and marine vertebrates and causes brucellosis. currently, there are nine recognized brucella species based on host preferences and phenotypic differences. the availability of 10 different genomes consisting of two chromosomes and representing six of the species allowed for a detailed comparison among themselves and relatives in the order rhizobiales. phylogenomic analysis of ortholog families show ... | 2009 | 19346311 |
| brucella canis endophthalmitis in 3 dogs: clinical features, diagnosis, and treatment. | to describe historical, clinical and diagnostic features of dogs with brucella canis endophthalmitis and the response to medical therapy. | 2009 | 19392878 |
| smooth brucella strains invade and replicate in human lung epithelial cells without inducing cell death. | inhalation is a common route for brucella infection. we investigated whether brucella species can invade and replicate within alveolar(a549) and bronchial (calu-6 and 16hbe14o-) human epithelial cells. the number of adherent and intracellular bacteria was higher for rough strains (brucella canis and brucella abortus rb51) than for smooth strains (b. abortus 2308 and brucella suis 1330). only smooth strains exhibited efficient intracellular replication (1.5-3.5 log increase at 24 h p.i.). a b. ab ... | 2009 | 19397873 |
| molecular typing for epidemiological evaluation of brucella abortus and brucella canis isolated in korea. | to investigate genotype relationships among regional groups of brucella isolates, variable-number tandem-repeat (vntr) analysis was conducted according to previously reported methods. field strains of brucella abortus and brucella canis were isolated from 9 provinces in the republic of korea during the years 1996-2006 and each of the isolates was classified by eight loci of hoof-prints. on the basis of the alleles, the 33 b. abortus and 21 b. canis field strains were divided into 22 and 18 disti ... | 2009 | 19463862 |
| a serological and bacteriological survey of dogs to detect brucella infection in lomas de zamora, buenos aires province. | canine brucellosis caused by brucella canis is a disease of the reproductive tract that may cause miscarriage in females, infection of the sexual organs in males and infertility in both sexes. the prevalence of brucellosis in dogs is unknown and little has been done to control the disease, except in certain breeds and some commercial dog kennels. in the course of a free neuter program in lomas de zamora, buenos aires province, prevalence of antibodies to brucella sp., bacteriological isolation a ... | 2009 | 19623899 |
| human brucella canis outbreak linked to infection in dogs. | the zoonotic risk of brucella canis has been considered fairly high for persons who handle breeding dogs in kennels or are exposed to infected animals. transmission to humans in other circumstances has been thought to be rare. we describe an uncommon outbreak of brucellosis caused by b. canis which, to the best of our knowledge, is the first reported in the literature. this outbreak involved six persons (three children and three adults), a bitch and three puppies which had close daily contact wi ... | 2010 | 19653929 |
| brucella canis causing infection in an hiv-infected patient. | from the blood culture of an hiv-positive patient with a febrile syndrome (cd4 count 385 cells/microl and viral load nondetectable), brucella canis was isolated. the patient was presumptively infected from his dogs, which tested positive, and showed good outcome after the therapy with doxycycline-ciprofloxacin, and the hiv infection would seem not to have been influenced by brucellosis. to our knowledge, no other case of b. canis in the setting of hiv infection has been reported in the literatur ... | 2010 | 19725766 |
| comparative performance of snp typing and 'bruce-ladder' in the discrimination of brucella suis and brucella canis. | two novel molecular assays, 'bruce-ladder' and snp typing, have recently been described designed to differentiate isolates of the genus brucella, causative organisms of the significant zoonotic disease brucellosis, at the species level. differentiation of brucella canis from brucella suis by molecular approaches can be difficult and here we compare the performance of 'bruce-ladder' and snp typing in correctly identifying b. canis isolates. both assays proved easy to perform but while 'bruce-ladd ... | 2010 | 19931322 |
| cholesterol, ganglioside gm1 and class a scavenger receptor contribute to infection by brucella ovis and brucella canis in murine macrophages. | the establishment of infection by brucella ovis and brucella canis in j774.a1 macrophages was found to be dependent upon cholesterol and ganglioside gm(1), two components of lipid rafts. this process also required a class a scavenger receptor of macrophages, and was not inhibited by smooth and rough lipopolysaccharides from brucella spp. in response to infection, both bacteria induced a weak degree of macrophage activation. these results demonstrate that b. ovis and b. canis use cell surface rec ... | 2010 | 20083220 |
| atp-binding cassette systems of brucella. | brucellosis is a prevalent zoonotic disease and is endemic in the middle east, south america, and other areas of the world. in this study, complete inventories of putative functional abc systems of five brucella species have been compiled and compared. abc systems of brucella melitensis 16m, brucella abortus 9-941, brucella canis rm6/66, brucella suis 1330, and brucella ovis 63/290 were identified and aligned. high numbers of abc systems, particularly nutrient importers, were found in all brucel ... | 2010 | 20169092 |
| immunoglobulin profiles in acute brucellosis experimentally induced by brucella canis in balb/c mice. | this study evaluated profiles of immunoglobulin (ig; iga, igg, igg1, and igg2a) response in experimental brucellosis induced with brucella canis in balb/c mice during an 8-week infection period. six- to 8-week-old balb/c mice (n = 36) were experimentally infected with 1 × 10(9) cfu of b. canis via the intraperitoneal route. serial serum samples were collected from the mice at 0, 3, 7, 14, 21, 28, 35, 42, 49, and 56 days after inoculation. the sera were tested by the rapid slide agglutination tes ... | 2010 | 20420532 |
| detection of brucella canis from inguinal lymph nodes of naturally infected dogs by pcr. | the aim of this study was to standardize and evaluate a pcr assay for the detection of brucella canis (b. canis) in lymph node samples of naturally infected dogs. the performance of the pcr was compared with the results of bacteriological culture as reference method. forty-eight inguinal lymph node samples were collected from 48 dogs (18 males and 30 females) that died in the city's pound in the years 2007-2008 and were examined by microbiological culture and the pcr assay. b. canis was isolated ... | 2010 | 20452003 |
| the feasibility of using antigens prepared with rough brucella strains for diagnosis of canine brucellosis. | clinical diagnosis of canine brucellosis is not sensitive enough and a negative blood culture cannot rule out the disease. indirect methods of serological testing such as agar gel immunodiffusion (agid), rapid slide agglutination test (rsat) and indirect enzyme linked immunoassay (ielisa) are preferred for routine diagnosis. since brucella canis shares antigenic components with the brucella ovis and brucella abortus rb51 strain, it would seem that either strain could be used as antigen. we prese ... | 2010 | 20461292 |
| human brucella canis infections diagnosed by blood culture. | | 2010 | 20587207 |
| comparison of a pcr assay in whole blood and serum specimens for canine brucellosis diagnosis. | the performance of a serum pcr assay was compared with that of a blood pcr assay for the diagnosis of canine brucellosis caused by brucella canis in 72 dogs. the dogs were classified into three groups (infected, non-infected and suspected brucellosis) according to the results of blood culture and serological tests. the sensitivities of blood pcr and serum pcr were, respectively, 97.14 per cent and 25.71 per cent. the specificities of both were 100 per cent. in the group of dogs with suspected br ... | 2010 | 20643887 |
| validation of an elisa method for the serological diagnosis of canine brucellosis due to brucella canis. | in the present study, the validation of an enzyme-linked immunosorbent assay (elisa) for serodiagnosis of canine brucellosis is described. two different antigenic extracts, obtained by heat or ultrasonic homogenization of microbial antigens from a wild isolate of brucella canis bacteria, were compared by elisa and western blot (wb). a total of 145 canine sera were used to define sensitivity, specificity and accuracy of the elisa as follows: (1) sera from 34 animals with natural b. canis infectio ... | 2011 | 20692004 |
| genomic island 2 is an unstable genetic element contributing to brucella lipopolysaccharide spontaneous smooth-to-rough dissociation. | brucella is a gram-negative bacterium that causes a worldwide-distributed zoonosis. the genus includes smooth (s) and rough (r) species that differ in the presence or absence, respectively, of the o-polysaccharide of lipopolysaccharide. in s brucellae, the o-polysaccharide is a critical diagnostic antigen and a virulence determinant. however, s brucellae spontaneously dissociate into r forms, a problem in antigen and s vaccine production. spontaneous r mutants of brucella abortus, brucella melit ... | 2010 | 20952568 |
| detection of brucella canis in a dog in italy. | brucella spp. is a worldwide zoonotic pathogen. infection by brucella canis in dogs is endemic in the southern usa and in central and south america, but it appears sporadically in other parts of the world, including europe. tissue samples from a dog with chronic prostatitis, discospondylitis and locomotor problems were subjected to clinical and laboratory examinations. b. canis was detected by pcr in biological fluids and tissues of the animal, while antibodies to b. canis were found in the seru ... | 2010 | 21213592 |
| detection of brucella canis-induced reproductive diseases in a kennel. | brucella spp. were isolated from an abortion case submitted for laboratory examination 8 months after the first clinical symptoms appeared in a kennel consisting of 31 dogs. pathological investigations revealed the parallel presence of necrotic placentitis and the strong immunostaining of trophoblast cells by immunohistochemistry (ihc) using hyperimmune rabbit anti-brucella canis primary antibodies. the rapid slide agglutination test was positive in 7 of 31 (23%) cases. the organism b. canis was ... | 2011 | 21217047 |
| brucella canis in a hiv-infected patient. | the true incidence of brucella canis in humans is unknown, but immunocompromised patients in direct contact with dogs are thought to be at a higher risk for infection. in this case report, we present a human case of b. canis in a patient infected with human immunodeficiency virus. | 2010 | 21306578 |
| genetic comparison of brucella canis isolates by the mlva assay in south korea. | the multiple-locus vntr analysis (mlva) assay is a method frequently employed as a molecular epidemiological tool for brucella genetic fingerprinting. the purpose of this study was to assess the genotyping of 77 b. canis isolates from 14 different dog breeding farms in korea by the mlva assay and to compare the epidemiological relationships between the korean isolates and foreign ones. simpson's diversity index for 17 loci showed a range from 0 to 0.846 in 77 b. canis isolates. b. canis isolates ... | 2011 | 21307620 |
| advanced multiplex pcr assay for differentiation of brucella species. | two new primer sets of a 766- and a 344-bp fragment were introduced into the conventional bruce-ladder pcr assay. this novel multiplex pcr assay rapidly and concisely discriminates brucella canis and brucella microti from brucella suis strains and also may differentiate all of the 10 brucella species. | 2011 | 21666028 |
| new bruce-ladder multiplex pcr assay for the biovar typing of brucella suis and the discrimination of brucella suis and brucella canis. | rapid and specific identification of brucella suis at the biovar level is necessary because some of the biovars that infect animals are pathogenic for humans. none of the molecular typing methods described so far are able to discriminate b. suis biovars in a single test and differentiation of b. suis from brucella canis by molecular approaches can be difficult. this article describes a new multiplex pcr assay, suis-ladder, for fast and accurate identification of b. suis at the biovar level and t ... | 2011 | 21782356 |
| seroprevalence of brucella abortus and b. canis in household dogs in southwestern nigeria: a preliminary report. | a preliminary serological study of 366 household dogs in lagos and ibadan, southwestern nigeria, was carried out to determine antibodies due to exposure to brucella abortus and b. canis, using the rose bengal test (rbt) and the rapid slide agglutination (rsa) test, respectively. results showed that 5.46 % (20/366) and 0.27 % (1/366) of the dogs screened were seropositive to b. abortus and b. canis, respectively. of all dogs, 36 had a history of being fed foetuses from cows and 11 (30.6 %) of the ... | 2011 | 21826840 |
| Reemergence and genetic comparison of Brucella canis in China, using a multiple-locus variable-number tandem-repeat assay. | | 2012 | 22024347 |
| [pet ownership and health status of pets from immunocompromised children, with emphasis in zoonotic diseases]. | to characterize pet ownership and pet health status in families of immunocompromised (is) children, with emphasis in zoonotic diseases. | 2011 | 21879144 |
| immunochemical characterization of antigens of brucella canis and their use in seroprevalence study of canine brucellosis. | to explore immunochemical characterization of antigens of brucella canis (b. canis), and the use in seroprevalence study of canine brucellosis. | 2011 | 22078946 |
| [investigation of brucella canis seropositivity by in-house slide agglutination test antigen in healthy blood donors]. | canine brucellosis which is due to brucella canis, is transmitted to man by infected dogs or their secretions. the symptoms of canine brucellosis are similar to the symptoms of brucellosis caused by other brucella species and endocarditis or meningitis may develop in untreated cases. there is limited data regarding b.canis infections in man and the current status of the disease is insufficiently evaluated in our country. serological diagnosis of brucellosis is classically based on standard slide ... | 2011 | 22090296 |
| a serological diagnostic survey for brucella canis infection in turkish patients with brucellosis-like symptoms. | the incidence of brucella canis infection in humans is unknown in turkey. in this study, we investigated the prevalence of b. canis infection in human sera obtained from six regions in turkey and comparatively evaluated the results obtained by agglutination-based techniques using standardized antigens made from b. canis. the patients (n = 1,746) presented with clinical symptoms that were similar to those of brucellosis. all patients who tested negative in the rose bengal test for the smooth ... | 2011 | 22116333 |
| oxidant and antioxidant parameters in patients with brucella canis. | objectives: the aim of this study was to evaluate the oxidative status in patient with brucella canis (b. canis) and healthy controls by measurement of total antioxidant capacity (tac) and total oxidant capacity (tos), and calculation of oxidative stress index (osi). design and methods: six patients with b. canis and 6 healthy control subjects were included in this study. diagnosis of b. canis was made by rapid slide agglutination test (rsat) and modified plate agglutination test (mpat). the ser ... | 2012 | 22266398 |
| the first detection of brucella canis in cattle in the republic of korea. | twenty mammary lymph node samples were collected from cattle on a farm in the republic of korea. these cattle were serologically negative for brucella by tube agglutination test (≤ 1:50) and serum agglutination test (≤ 1:50). out of 20 lymph node samples, two samples were positive for brucella growth on brucella agar as well as blood agar. tests for urease, hydrogen sulphide and reactions against monospecific sera a and m indicated that these two isolates (no. 15 and 16) belong to the genus bruc ... | 2011 | 22339983 |
| [canine brucellosiss: seroprevalence and risk factors in pets from eleven neighbourhoods in medellin, colombia]. | objective: determining brucella canis prevalence in domestic dogs (pets) from 11 districts in medellín. a survey of risk factors was also carried out. materials and methods: immunoassay was used for analysing441 dog serum samples and several risk factors regarding their owners and some related to the immediate environment were established. results: brucellosis prevalence was 2.76 % (1.11-4.42 95 %ci), being highestin the buenos aires (6.9 %) and villa hermosa districts (5.7%). seroprevalence was ... | 2012 | 23912517 |
| identification of the virb operon genes encoding the type iv secretion system, in colombian brucella canis isolates. | canine brucellosis is a zoonotic disease caused by brucella canis. the establishment of intracellular replicative niches of b. canis is mediated by proteins secreted by the type iv secretion system, which is encoded by the virb operon. the characterization of such genes has been conducted in other species of the genus, but not in b. canis. we report the design of a multiplex pcr test for the detection of the virb operon genes of b. canis. primers for each of the 12 genes were designed and evalua ... | 2012 | 23290573 |
| brucella canis infection in dogs attended in veterinary clinics from patos, parabaība state, brazil. | to determine the frequency of anti-brucella canis antibodies in dogs attended in veterinary clinics from patos, paraíba state, brazil, as well as to identify risk factors and to isolate and identify the agent, 193 dogs were used. agar gel immunodiffusion test (agid) was used to detect b. canis antibodies in sera. isolation of b. canis was carried out in blood and bone marrow from seropositive animals. six animals tested seropositive in agid, resulting in a frequency of 3.11%. b. canis was isolat ... | 2011 | 24031770 |
| serological trail of brucella infection in an urban slum population in brazil. | brucellosis is a re-emerging zoonosis with new cases reported each year in many latin american countries, but it is mostly under-recognized. this study presents a serological investigation of infection with brucella abortus and brucella canis in a poor urban community in the city of salvador, brazil. | 2012 | 23000868 |
| complete genome sequence of brucella canis strain hsk a52141, isolated from the blood of an infected dog. | brucella canis infection can be clinically inapparent in dogs, and when infection goes unnoticed, there is a chance for dog-to-human transmission. a new strain of b. canis was isolated from the blood of an infected dog in order to analyze the pathogenic mechanism, compare genetic properties, and develop new genetic tools for early diagnosis of canine brucellosis. herein, we report the complete genome sequence of the strain b. canis hsk a52141. this is the second complete genome sequence and biol ... | 2012 | 22933762 |
| the first case of brucella canis in sweden: background, case report and recommendations from a northern european perspective. | infection with brucella canis has been diagnosed in sweden for the first time. it was diagnosed in a three-year-old breeding bitch with reproductive disturbances. fifteen in-contact dogs were tested repeatedly and all of them were negative for b. canis. the source of infection could not be defined. the present article describes the case and the measures undertaken and gives a short review over b. canis. recommendations on how to avoid the infection in non-endemic countries are given. | 2012 | 22452858 |
| toxoplasmosis, leptospirosis and brucellosis in stray dogs housed at the shelter in umuarama municipality, paraná, brazil. | leptospirosis, toxoplasmosis and brucellosis are diseases with worldwide distribution. among stray dogs, these zoonoses are facilitated by direct contact with other animal species, by the habit of scavenging garbage and hunting in search of food, drinking standing water, smelling other animals' urine, licking female genitalia and the sexual act itself. the objective of this study was to detect antibodies anti-toxoplasma gondii, anti-leptospira spp., anti-brucella canis and anti-brucella abortus ... | 2013 | 24066949 |
| brucella canis is an intracellular pathogen that induces a lower proinflammatory response than smooth zoonotic counterparts. | canine brucellosis caused by brucella canis is a disease of dogs and a zoonotic risk. b. canis harbors most of the virulence determinants defined for the genus, but its pathogenic strategy remains unclear since it has not been demonstrated that this natural rough bacterium is an intracellular pathogen. studies of b. canis outbreaks in kennel facilities indicated that infected dogs displaying clinical signs did not present hematological alterations. a virulent b. canis strain isolated from those ... | 2015 | 26438796 |
| evaluation of the efficacy of outer membrane protein 31 vaccine formulations for protection against brucella canis in balb/c mice. | canine brucellosis is an infectious disease caused by the gram-negative bacterium brucella canis. unlike conventional control programs for other species of the genus brucella, currently there is no vaccine available against canine brucellosis, and preventive measures are simply diagnosis and isolation of infected dogs. new approaches are therefore needed to develop an effective and safe immunization strategy against this zoonotic pathogen. in this study, balb/c mice were subcutaneously immunized ... | 2014 | 25339409 |
| human brucella canis infection and subsequent laboratory exposures associated with a puppy, new york city, 2012. | human brucella canis infection incidence is unknown. most identified cases are associated with pet dogs. laboratory-acquired infections can occur following contact with brucella spp. we identified a paediatric b. canis case, the source and other exposed persons. a 3-year-old new york city child with fever and dyspnoea was hospitalized for 48 h for bronchiolitis. after her admission, blood culture grew b. canis, she was prescribed anti-microbials and recovered. b. canis was also isolated from blo ... | 2014 | 25363807 |
| identification of highly pathogenic microorganisms by matrix-assisted laser desorption ionization-time of flight mass spectrometry: results of an interlaboratory ring trial. | in the case of a release of highly pathogenic bacteria (hpb), there is an urgent need for rapid, accurate, and reliable diagnostics. maldi-tof mass spectrometry is a rapid, accurate, and relatively inexpensive technique that is becoming increasingly important in microbiological diagnostics to complement classical microbiology, pcr, and genotyping of hpb. in the present study, the results of a joint exercise with 11 partner institutions from nine european countries are presented. in this exercise ... | 2015 | 26063856 |
| serosurvey for selected pathogens in free-ranging american black bears (ursus americanus) in maryland, usa. | american black bears (ursus americanus) in maryland, usa, live in forested areas in close proximity to humans and their domestic pets. from 1999 to 2011, we collected 84 serum samples from 63 black bears (18 males; 45 females) in five maryland counties and tested them for exposure to infectious, including zoonotic, pathogens. a large portion of the bears had antibody to canine distemper virus and toxoplasma gondii, many at high titers. prevalences of antibodies to zoonotic agents such as rabies ... | 2014 | 25075540 |
| isolation of aureimonas altamirensis, a brucella canis-like bacterium, from an edematous canine testicle. | microbiological and histological analysis of a sample from a swollen testicle of a 2-year-old border collie dog revealed a mixed infection of the fungus blastomyces dermatitidis and the gram-negative bacterium aureimonas altamirensis. when subjected to an automated microbial identification system, the latter isolate was provisionally identified as psychrobacter phenylpyruvicus, but the organism shared several biochemical features with brucella canis and exhibited agglutination, albeit weakly, wi ... | 2014 | 25292192 |
| activation of bovine neutrophils by brucella spp. | brucellosis is a globally important zoonotic infectious disease caused by gram negative bacteria of the genus brucella. while many species of brucella exist, brucella melitensis, brucella abortus, and brucella suis are the most common pathogens of humans and livestock. the virulence of brucella is largely influenced by its ability to evade host factors, including phagocytic killing mechanisms, which are critical for the host response to infection. the aim of this study was to characterize the bo ... | 2016 | 27436438 |
| booster vaccination with safe, modified, live-attenuated mutants of brucella abortus strain rb51 vaccine confers protective immunity against virulent strains of b. abortus and brucella canis in balb/c mice. | brucella abortus attenuated strain rb51 vaccine (rb51) is widely used in prevention of bovine brucellosis. although vaccination with this strain has been shown to be effective in conferring protection against bovine brucellosis, rb51 has several drawbacks, including residual virulence for animals and humans. therefore, a safe and efficacious vaccine is needed to overcome these disadvantages. in this study, we constructed several gene deletion mutants (δcydc, δcydd and δpurd single mutants, and δ ... | 2015 | 26341622 |
| survey for infectious disease in the south american fur seal (arctocephalus australis) population at punta san juan, peru. | the peruvian population of the south american fur seal ( arctocephalus australis ) is a distinct evolutionarily significant unit that is endangered. one of the largest rookeries for this species in peru is located within the punta san juan marine protected area (15°22's, 75°12'w). to better understand the current health status of this population, exposure to 10 pinniped pathogens was evaluated in adult female fur seals (n=29) via serology and polymerase chain reaction (pcr) techniques in novembe ... | 2015 | 26056875 |
| parasitology and serology of free-ranging coyotes (canis latrans) in north carolina, usa. | coyotes (canis latrans) have expanded recently into the eastern us and can serve as a source of pathogens to domestic dogs (canis lupus familiaris), livestock, and humans. we examined free-ranging coyotes from central north carolina, us, for selected parasites and prevalence of antibodies against viral and bacterial agents. we detected ticks on most (81%) coyotes, with amblyomma americanum detected on 83% of those with ticks. fifteen (47%) coyotes were positive for heartworms (dirofilaria immiti ... | 2015 | 25984773 |
| advancement of knowledge of brucella over the past 50 years. | fifty years ago, bacteria in the genus brucella were known to cause infertility and reproductive losses. at that time, the genus was considered to contain only 3 species: brucella abortus, brucella melitensis, and brucella suis. since the early 1960s, at least 7 new species have been identified as belonging to the brucella genus (brucella canis, brucella ceti, brucella inopinata, brucella microti, brucella neotomae, brucella ovis, and brucella pinnipedialis) with several additional new species u ... | 2014 | 24981716 |
| a repa-based elisa for discriminating cattle vaccinated with brucella suis 2 from those naturally infected with brucella abortus and brucella melitensis. | the commonest ways of diagnosing brucellosis in animals include the rose-bengal plate agglutination test, the buffered plate agglutination test (bpa), the slide agglutination test, the complement fixation test, and the indirect enzyme linked immunosorbent assay (i-elisa). however, these methods cannot discriminate the brucella vaccine strain (brucella suis strain 2; b. suis s2) from naturally acquired virulent strains. of the six common brucella species, brucella melitensis, brucella abortus, an ... | 2014 | 24941369 |
| serological survey of brucella canis in dogs in urban harare and selected rural communities in zimbabwe. | a cross-sectional study was conducted in order to detect antibodies for brucella canis (b. canis) in dogs from urban harare and five selected rural communities in zimbabwe. sera from randomly selected dogs were tested for antibodies to b. canis using an enzyme-linked immunosorbent assay. overall, 17.6% of sera samples tested (57/324, 95% ci: 13.5-21.7) were positive for b. canis antibodies. for rural dogs, seroprevalence varied from 11.7% - 37.9%. rural dogs recorded a higher seroprevalence (20. ... | 2014 | 24830899 |
| serological survey of <i>brucella canis</i> in dogs in urban harare and selected rural communities in zimbabwe. | a cross-sectional study was conducted in order to detect antibodies for brucella canis (b. canis) in dogs from urban harare and five selected rural communities in zimbabwe. sera from randomly selected dogs were tested for antibodies to b. canis using an enzyme-linked immunosorbent assay. overall, 17.6% of sera samples tested (57/324, 95% ci: 13.5-21.7) were positive for b. canis antibodies. for rural dogs, seroprevalence varied from 11.7% - 37.9%. rural dogs recorded a higher seroprevalence (20. ... | 2014 | 28235300 |
| detection of brucella canis infection in dogs by blood culture and bacterial identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | brucella canis was recovered from dogs that were canine brucellosis suspect by blood culture using a modified lysis method. organism identity was established by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). the instrument-provided security library identified the isolates as brucella species. the isolates were further identified as b. canis with the help of phenotypic and genotypic characteristics. the mass spectral profiles from characterized b. can ... | 2017 | 28381135 |
| brucella canis infection in dogs from commercial breeding kennels in brazil. | canine brucellosis caused by brucella canis is a neglected zoonosis worldwide and is a leading cause of reproductive failure in dogs, often causing substantial economic losses in breeding kennels. this study aimed to investigate the occurrence of b. canis infection in dogs of commercial breeding kennels located in são paulo state, brazil. a total of 753 dogs (183 males and 570 females) from 38 commercial kennels were clinically examined, and blood samples were collected for brucellosis diagnosis ... | 2017 | 28296215 |
| genome sequences of three brucella canis strains isolated from humans and a dog. | brucella canis is a facultative intracellular pathogen that preferentially infects members of the canidae family. here, we report the genome sequencing of two brucella canis strains isolated from humans and one isolated from a dog host. | 2017 | 28232424 |
| polymeric antigen blsomp31 in aluminium hydroxide induces serum bactericidal and opsonic antibodies against brucella canis in dogs. | polymeric antigen blsomp31 is an immunogenic vaccine candidate that confers protection against brucella canis in mice. in this preliminary study, the immunogenicity and safety of blsomp31 adsorbed to aluminum hydroxide gel (blsomp31-ah) were evaluated in beagle dogs. in addition, the potential to elicit serum antibodies with complement-dependent bactericidal activity and/or to enhance phagocytosis by neutrophils were analyzed. dogs were immunized three times with blsomp31-ah by subcutaneous rout ... | 2017 | 28166930 |
| molecular assessment of the transplacental transmission of toxoplasma gondii, neospora caninum, brucella canis and ehrlichia canis in dogs. | given the fact that numerous microbial species can be detected in pregnant female dogs, the objective of this study was to assess the transplacental transmission of brucella canis, ehrlichia canis, neospora caninum and toxoplasma gondii in stillborn puppies. this study involved 41 stillborn puppies, 78.6% of which were positive for t. gondii, 52.4% for n. caninum and 59.5% for b. canis. e. canis was not detected in any of the analyzed puppies. pregnancy is an important physiological condition fo ... | 2016 | 27865263 |
| [a case of brucellosis with intermittent fever in a patient returning from france]. | we herein report on a 62-year-old man who presented with symptoms of intermittent fever that persisted after returning from a trip to france. during his trip, he had eaten natural cheese. although no bacteria could be isolated from blood culture, the serum agglutination test showed a positive antibody titer of 1 : 160 for brucella canis. the patient responded well to combination antibiotic therapy consisting of gentamicin, rifampicin, and doxycycline, and his symptoms improved. he became antibod ... | 2016 | 27197442 |
| one health in practice: a pilot project for integrated care of zoonotic infections in immunocompromised children and their pets in chile. | although pets provide physiological and psychological benefits to their owners, they are a potential source of zoonotic infections, especially for vulnerable individuals such as immunocompromised patients. during 1 year, we therefore performed a pilot project, which included 32 immunocompromised chilean children and their family pets (35 dogs and 9 cats) with the aim of detecting, treating and preventing zoonotic infections. children were examined by infectious diseases paediatricians and demogr ... | 2016 | 26684576 |
| evaluation of an immunochromatographic test to the diagnosis of canine brucellosis caused by brucella canis. | this study evaluated the performance of an immunochromatographic test (ict) for the diagnosis of canine brucellosis caused by brucella canis, comparing its results with that of the rapid slide agglutination test with and without the use of 2-mercaptoethanol and the agar gel immunodiffusion test (agid). the microbiological culture, pcr and clinical examination were used as reference. according to the results obtained in clinical examination, blood culture, culture of semen and vaginal swab and pc ... | 2015 | 26489371 |
| canid herpesvirus 1 (chv-1)-related disease in older puppies and chv-1 shedding in the vagina of adult pregnant dogs. | a large breeding kennel of bulldogs (n = 57) experienced several canid herpesvirus 1 (chv-1)-related diseases in older puppies (9 weeks of age) in arkansas. chv-1 has been repeatedly confirmed in the kennel in several animals for 3 years (january 2012-february 2015) using various virology tests. i was able to detect a partial sequence of chv dna (~120 bp) in archived formalin-fixed, paraffin-embedded tissue blocks after 3 years of storage. chv-1 is persistently circulating in this kennel in spit ... | 2015 | 26450840 |
| [brucella canis]. | | 2015 | 26065455 |
| comparison of brucella canis genomes isolated from different countries shows multiple variable regions. | brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years. in this study, we report the sequencing, annotation and analysis of the genome of brucella canis strain oliveri isolated from a dog in a breeding kennel in medellín, colombia, south america. whole genome shotgun sequencing was carried out using the roche 454 gs flx titanium technology at the national center for genomic sequencing-cnsg in medellin, colombia. the assembly procedure was ... | 2015 | 25820207 |
| identification of brucella spp. isolated from human brucellosis in malaysia using high-resolution melt (hrm) analysis. | molecular approaches have been investigated to overcome difficulties in identification and differentiation of brucella spp. using conventional phenotypic methods. in this study, high-resolution melt (hrm) analysis was used for rapid identification and differentiation of members of brucella genus. a total of 41 brucella spp. isolates from human brucellosis were subjected to hrm analysis using 4 sets of primers, which identified 40 isolates as brucella melitensis and 1 as brucella canis. the techn ... | 2015 | 25641125 |
| brucellosis outbreak in a swedish kennel in 2013: determination of genetic markers for source tracing. | brucellosis is a highly infectious zoonotic disease but rare in sweden. nonetheless, an outbreak of canine brucellosis caused by an infected dog imported to sweden was verified in 2013. in total 25 dogs were tested at least duplicated by the following approaches: real-time pcr for the detection of brucella canis, a brucella genus-specific real-time pcr, selective cultivation, and microscopic examination. the whole genome of b. canis strain sva13 was analysed regarding genetic markers for epidemi ... | 2014 | 25465667 |
| a new brucella canis species-specific pcr assay for the diagnosis of canine brucellosis. | brucellosis is a zoonotic disease that is transmitted from animals to humans, and the development of a rapid, accurate, and widely available identification method is essential for diagnosing this disease. in this study, we developed a new brucella canis species-specific (bcss) pcr assay and evaluated its specificity and sensitivity. a specific pcr primer set was designed based on the bcan_b0548-0549 region in chromosome ii of b. canis. the pcr detection for b. canis included amplification of a 3 ... | 2014 | 25128932 |
| whole-genome sequence of brucella canis strain sva13, isolated from an infected dog. | an outbreak of canine brucellosis in sweden was confirmed by the national veterinary institute (sva) in august 2013. the whole genome of the causative agent was sequenced, assembled, and analyzed. | 2014 | 25035330 |
| evidence for unapparent brucella canis infections among adults with occupational exposure to dogs. | human serological assays designed to detect brucellosis will miss infections caused by brucella canis, and low levels of periodic bacteremia limit diagnosis by blood culture. recent b. canis outbreaks in dogs and concomitant illnesses in caretakers suggest that unapparent human infections may be occurring. with more than a quarter of a million persons in occupations involving dogs, and nearly 80 million dog owners in the united states, this pathogen is an under-recognized human health threat. to ... | 2014 | 24751191 |
| genetic polymorphism characteristics of brucella canis isolated in china. | in china, brucellosis is an endemic disease typically caused by brucella melitensis infection (biovars 1 and 3). brucella canis infection in dogs has not traditionally recognized as a major problem. in recent years however, brucellosis resulting from brucella canis infection has also been reported, suggesting that infections from this species may be increasing. data concerning the epidemiology of brucellosis resulting from brucella canis infection is limited. therefore, the purpose of this study ... | 2014 | 24465442 |
| microplate agglutination test for canine brucellosis using recombinant antigen-coated beads. | brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. the diagnosis of canine brucellosis is based on bacteriological examination and serological methods, including agglutination and gel diffusion tests. in this study, four recombinant antigens, heat shock protein 60, rhizopine-binding protein, cu-zn superoxide dismutase, and hypothetical protein (ag 4), were constructed. these antigens were coated on latex beads and their usefulness in the serologic ... | 2014 | 27355048 |
| [detection of brucella canis by immunochromatography method in vague dogs captured in temuco city, chile, 2011]. | brucella canis is responsible for brucellosis in dogs, causing reproductive disorders and is considered a zoonoses, as described in several countries. the epidemiological data are scarce in our country. | 2013 | 24248108 |
| disease control through fertility control: secondary benefits of animal birth control in indian street dogs. | we sought to (1) survey sexually intact street dogs for a wide range of diseases in three cities in rajasthan, india and (2) evaluate links between the health of non-treated dogs and both the presence and duration of animal birth control (abc) programs. abc regimes sterilize and vaccinate stray dogs in an attempt to control their population and the spread of rabies. they are commonly suggested to improve the health of those dogs they serve, but here we provide evidence that these benefits also e ... | 2014 | 24239212 |
| [factors associated with brucella canis seropositivity in kennels of two regions of antioquia, colombia]. | the objectives of this study were to determine brucella canis seroprevalence in dogs and in humans living near kennels and to explore risk factors associated with seropositivity. twenty kennels were included in a serological survey with rsat-2me, and samples were collected from 428 dogs and 91 humans. an interview was applied to determine risk factors, and the data were analyzed using logistic regression. seroprevalence was 15% in dogs and 9% in humans. factors associated with current canine ser ... | 2013 | 24127092 |
| report of wood decay fungus inonotus tropicalis (phylum basidiomycota) from a dog with a granulomatous mediastinal mass. | a 75.9-kg, 3.5-year-old male irish wolfhound dog with a 2-3-week history of gagging and eating difficulties was referred to the university of florida veterinary medical hospital (gainesville, florida) for evaluation of a large cranial mediastinal mass suspected to be a thymoma or lymphosarcoma. the patient had 4 months of nearly 10 kg progressive weight loss with severe flank sensitivity and radiographically apparent lumbar vertebral changes interpreted as discospondylitis. lab work revealed hyp ... | 2013 | 23929678 |
| the vaccine candidate blsomp31 protects mice against brucella canis infection. | canine brucellosis represents a major reproductive problem worldwide and it is considered a zoonotic disease. new approaches are therefore urgently needed to develop an effective and safe immunization strategy against brucella canis. in the present study, balb/c mice were subcutaneously immunized with the recombinant chimera rblsomp31 formulated in different adjuvants. the different strategies induced a vigorous immunoglobulin g (igg) response, with high titers of igg1 as well as igg2. besides, ... | 2013 | 23906889 |
| brucella canis peritonitis. | although brucellosis is a common zoonotic disease worldwide, infection with brucella canis is rare and often difficult to diagnose. we describe the clinical and epidemiological aspects of a case of b. canis peritonitis. | 2014 | 23846518 |
| within-host evolution of brucella canis during a canine brucellosis outbreak in a kennel. | little is currently known about brucella evolution within the host during infection. the current study is the first to employ fine-scale genotyping on an isolate collection derived from a brucella canis outbreak. eight isolates of b. canis, cultured from different tissues of three dogs (female, stud dog, puppy of another female) from a single kennel over three months were genetically characterized with a 15-marker multi-locus, variable-number tandem repeat (vntr) analysis (mlva) to assess the ge ... | 2013 | 23587163 |
| [brucella canis endocarditis: first documented case in argentina]. | we herein present the case of an adult male patient who consulted for lower extremity edema, a 2- month history of fever and oppressive chest pain radiating to the left arm. he referred neither contact with breeding animals nor consumption of unpasteurized dairy products. a diagnosis of endocarditis was confirmed by cardiac studies. since the empirical treatment with cephalotin, ampicillin and gentamicin failed, the patient underwent aortic valve replacement. a total of four blood cultures were ... | 2015 | 23560789 |
| differential expression of iron acquisition genes by brucella melitensis and brucella canis during macrophage infection. | brucella spp. cause chronic zoonotic disease often affecting individuals and animals in impoverished economic or public health conditions; however, these bacteria do not have obvious virulence factors. restriction of iron availability to pathogens is an effective strategy of host defense. for brucellae, virulence depends on the ability to survive and replicate within the host cell where iron is an essential nutrient for the growth and survival of both mammalian and bacterial cells. iron is a par ... | 2012 | 22403618 |
| brucella canis in a dog in the uk. | | 2017 | 28408516 |
| genotyping brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (mlva) assay. | differentiation of brucella canis from other brucella species are mainly performed through pcr-based methods and multilocus variable-number tandem-repeat (vntr) analysis (mlva) procedures. both pcr-based and mlva methods are limited in discriminating b. canis strains. a new mlva-13bc method for b. canis genotyping was established by combining eight newly-developed vntrs with five published ones. during 2010 and 2016, 377 b. canis pcr-positives were identified from 6,844 canine blood samples from ... | 2017 | 28432327 |
| a safe and molecular-tagged brucella canis ghosts confers protection against virulent challenge in mice. | canine brucellosis, caused by brucella canis, is a persistent infectious reproductive disease in dogs. the absence of effective treatment to the intracellular pathogen and the irreversible consequence of infection makes the need of a specific vaccine urgent. bacterial ghosts (bgs) are the empty envelopes of bacteria with no genome content inside, which emerge as a proper vaccine candidate due to its intact outer antigen. it is generally derived from a genetically engineered strain, through the e ... | 2017 | 28532790 |
| bacterial reproductive pathogens of cats and dogs. | with the notable exception of brucella canis, exogenous bacterial pathogens are uncommon causes of reproductive disease in cats and dogs. most bacterial reproductive infections are endogenous, and predisposing factors for infection are important. this article reviews the etiology, pathogenesis, clinical presentation, diagnosis, treatment, and public health significance of bacterial reproductive pathogens in cats and dogs. | 2012 | 22482819 |
| immunogenic response of brucella canis virb10 and virb11 mutants in a murine model. | the virb locus, which encodes the type iv secretion system, is a major component of virulence in brucella. a non-polar virb10 mutant and a virb11 deletion mutant were constructed in brucella canis. in the mouse model, both mutants were cleared at day 21 post-infection, indicating reduced virulence in mice. after challenging with wild-type b. canis, the amounts of cfu recovered at day 15 were significantly lower in the group previously vaccinated with the virb10 mutant. levels of igg1, igg2a, igg ... | 2012 | 22919627 |
| recent trends in human brucella canis infection. | there is little information in the literature regarding the clinical progress of brucellosis in patients affected by other diseases. we report brucella canis human infection link to gaucher's disease and guillain barré syndrome and discuss complications observed in a case with infective endocarditis. the three cases described came from areas of socio-economic deprivation and scarce epidemiological information where the healthcare personnel did not even consider such diagnosis. the growth of larg ... | 2013 | 23040615 |
| first detection of brucella canis infections in a breeding kennel in austria. | brucella canis occurs almost worldwide and is a potential danger to the health of dogs and humans. the pathogen was detected in the placenta and fetuses of a standard poodle by direct culture and immunohistochemistry. further, brucellae were also isolated from the blood samples of two asymptomatic female medium poodles. the isolates were identified as b. canis by conventional microbiological methods and a novel bruce-ladder multiplex pcr. genotyping was performed by multiple locus variable numbe ... | 2012 | 23109021 |
| complete genome sequence of brucella canis strain 118, a strain isolated from canine. | brucella canis infects several species of animals, and canine is the preferred host. genome sequences of strains from different hosts are valuable for comparative analysis of host adaptation and microevolution. here, we report the genome sequence of brucella canis strain 118, a strain isolated from canine. | 2012 | 23144418 |
| second uk isolation of brucella canis. | | 2017 | 28646065 |
| complete genome sequence of brucella canis bcb018, a strain isolated from a human patient. | brucella canis is considered a rare cause of human brucellosis because of difficulties in presumptive diagnosis and underestimation of the incidence. here, we report the draft genome sequence of a brucella canis isolate, bcb018, isolated from a human patient, providing precious resources for comparative genomics analysis of brucella field strains. | 2012 | 23144429 |
| newly identified variability in brucella canis fatty-acid content is associated with geographical origin. | this study compared the fatty-acid profiles of brucella canis blood culture isolates obtained from infected dogs in the uk, germany, japan, south africa, peru, mexico, colombia, and argentina, and from a human clinical case in argentina, to a bank of isolates obtained from canine outbreaks in the usa. analysis of a total of 42 b. canis isolates and one reference strain found a marked variation within the species. fatty-acid analysis showed that only the isolates from argentina, colombia, and mex ... | 2013 | 23174310 |
| [investigation of brucella canis seroprevalence in brucellosis suspected cases]. | brucella canis which is the main etiologic agent of brucellosis in dogs, can be transmitted to man. it causes mild or asymptomatic infection in human compared with other brucella species. b.canis can be transmitted to man either by laboratory accidents or contact with infected dogs. since b.canis infections in humans are not routinely investigated in hospitals in turkey, the data are limited to reveal the current status of b.canis infections in people in our country. the purpose of this study wa ... | 2013 | 23390913 |