vp6 gene diversity in 11 brazilian strains of porcine group c rotavirus. | porcine group c rotavirus (rvc) is recognised as an enteric pathogen in piglets worldwide. the vp6 gene of rvc is divided into seven i-genotypes. genotypes i2 and i3 are found in human and bovine strains, respectively; the porcine strains are divided into the other five genotypes (i1, i4-i7). in this study, molecular analysis of nearly the full length of the vp6 gene was performed in 11 brazilian wild-type porcine rvc strains identified in diarrhoeic faecal samples, which were collected from eig ... | 2015 | 25331342 |
nucleotide correlations between rotavirus c isolates in clinical samples from outbreaks and in sewage samples. | rotavirus c (rvc) is detected in both sporadic cases and outbreaks of gastroenteritis worldwide. however, the epidemic dynamics of rvc in populations remain poorly understood because the detection rate is low. in this study, raw sewage samples were collected from a wastewater treatment plant in yokohama, japan, over 5 years, in 12-month period from september to august, to identify the rvc strains in these samples and compare them with the rvc strains circulating in the population. rvc strains we ... | 2015 | 25475764 |
genetic analysis of human rotavirus c: the appearance of indian-bangladeshi strain in far east asian countries. | rotaviruses c (rvcs) circulate worldwide as an enteric pathogen in both humans and animals. most studies of their genetic diversity focus on the vp7 and vp4 genes, but the complete genomes of 18 human rvcs have been described in independent studies. the genetic background of the far east asian rvcs is different than other human rvcs that were found in india and bangladesh. recently, a rvc detected in 2010 in south korea had genetic background similar to the indian-bangladeshi rvcs. this study wa ... | 2016 | 27071530 |
detection of enteric viruses in fecal specimens from nonbacterial foodborne gastroenteritis outbreaks in tokyo, japan between 1966 and 1983. | we investigated the prevalence of 5 enteric viruses (norovirus [nov], sapovirus, rotavirus, astrovirus, and adenovirus) in archived stool specimens collected from 70 foodborne gastroenteritis outbreaks in tokyo, japan, which occurred from 1966 to 1983, and genetically characterized these viruses. nov was detected in 48 (68.6%) outbreaks, while sav, group c rotavirus (rvc), and astrovirus were detected in 1 (1.4%) outbreak each. based on the partial capsid sequences, the detected novs were classi ... | 2017 | 27357976 |
multiplex real-time pcr assays for the detection of group c rotavirus, astrovirus, and subgenus f adenovirus in stool specimens. | group c rotavirus (gcrv), astrovirus (astv), and adenovirus (subgenus f adenov) are etiologic agents of acute nonbacterial gastroenteritis, which often represents community outbreaks. for the efficient detection of gcrv, astv, and subgenus f adenov in stool specimens, a multiplex real-time pcr assay was developed to detect these three viruses simultaneously, with high sensitivity and specificity. in total, 8404 clinical specimens were collected between april 2008 and march 2011 and tested for gc ... | 2013 | 23159674 |
rotavirus in adults, brazil, 2004-2011: g2p[4] dominance and potential impact on vaccination. | the aim of this study was to monitor rotavirus (rv) infections in adults >18 years with acute gastroenteritis during 2004-2011 national brazilian rv surveillance. in addition, to characterize the rv group a (rva) strains in order to gain insight into the supposed vaccine selective pressure imposed to brazilian children population. | 2014 | 24076114 |
epidemiological and genetic analysis of human group c rotaviruses isolated from outbreaks of acute gastroenteritis in yokohama, japan, between 2006 and 2012. | group c rotavirus (gcrv) infection has been described in several parts of the world, predominantly as sporadic cases of acute gastroenteritis. little is known about the yearly changes in the gcrv strains from diarrheal outbreaks. stool samples collected from outbreaks of acute gastroenteritis in yokohama, japan, between 2006 and 2012 that were negative for norovirus, sapovirus, and group a rotavirus, were screened for gcrv using a reverse passive hemagglutination method. the gcrv strains were ch ... | 2014 | 24154950 |
prevalence of rotaviruses groups a and c in egyptian children and aquatic environment. | the objective of this study is to compare the prevalence of rotaviruses groups a and c in egyptian children and aquatic environment. from 110 stool specimens of children with acute diarrhea and using rt-pcr, 35 samples (31.8 %) were positive for human rotavirus group a and 15 samples (13.6 %) were positive for human rotavirus group c. from 96 samples collected from zenin wastewater treatment plant over a 2-year period (november 2009-october 2011) and using rt-pcr, rotavirus group a was detected ... | 2015 | 25665825 |
modeling of the rotavirus group c capsid predicts a surface topology distinct from other rotavirus species. | rotavirus c (rvc) causes sporadic gastroenteritis in adults and is an established enteric pathogen of swine. because rvc strains grow poorly in cell culture, which hinders generation of virion-derived rvc triple-layered-particle (tlp) structures, we used the known rotavirus a (rva) capsid structure to model the human rvc (bristol) capsid. comparative analysis of rva and rvc capsid proteins showed major differences at the vp7 layer, an important target region for vaccine development due to its an ... | 2016 | 26524514 |
genetic heterogeneity of the vp6 gene and predominance of g6p[5] genotypes of brazilian porcine rotavirus c field strains. | the aim of this study was to investigate the genetic diversity of the vp6, vp7, and vp4 genes of 15 brazilian wild-type porcine rvc strains identified in diarrheic fecal samples. the vp6 gene analysis demonstrated heterogeneity between the 15 rvc strains, which clustered in three distinct genotypes (i1, i5, and i6). in the vp7 and vp4 gene analysis, the genotype combination g6p[4] was detected in only one strain (uel-77), while g6p[5] was the most commonly (n = 14) detected in rvc strains identi ... | 2016 | 26758732 |
characterization of a genetically heterogeneous porcine rotavirus c, and other viruses present in the fecal virome of a non-diarrheic belgian piglet. | next-generation sequencing (ngs) technologies are becoming increasingly accessible, leading to an expanded interest in the composition of the porcine enteric virome. in the present study, the fecal virome of a non-diarrheic belgian piglet was determined. although the virome of only a single piglet was analyzed, some interesting data were obtained, including the second complete genome of a pig group c rotavirus (rvc). this belgian strain was only distantly related to the only other completely cha ... | 2016 | 27184192 |
etiological study of enteric viruses and the genetic diversity of norovirus, sapovirus, adenovirus, and astrovirus in children with diarrhea in chongqing, china. | enteric viruses are a major cause of diarrhea in children, especially those <5 years old. identifying the viral agents is critical to the development of effective preventive measures. this study aimed to determine the prevalence of common enteric viruses in children <5 years old presented with diarrhea to the children's hospital of chongqing medical university. | 2013 | 24004442 |
development of a multiplex real-time pcr assay for detection of human enteric viruses other than norovirus using samples collected from gastroenteritis patients in fukui prefecture, japan. | there are many varieties of gastroenteritis viruses, of which norovirus (nov) accounts for over 90% of the viral food poisoning incidents in japan. however, protocols for rapidly identifying other gastroenteritis viruses need to be established to investigate nov-negative cases intensively. in this study, a multiplex real-time pcr assay targeting rotavirus a, rotavirus c, sapovirus, astrovirus, adenovirus, and enterovirus was developed using stool samples collected from gastroenteritis patients b ... | 2017 | 28845896 |
distantly related rotaviruses in common shrews, germany, 2004-2014. | we screened samples from common shrews (sorex araneus) collected in germany during 2004-2014 and identified 3 genetically divergent rotaviruses. virus protein 6 sequence similarities to prototype rotaviruses were low (64.5% rotavirus a, 50.1% rotavirus c [tentative species k], 48.2% rotavirus h [tentative species l]). shrew-associated rotaviruses might have zoonotic potential. | 2019 | 31742508 |
reassortant human group c rotaviruses in hungary. | | 2015 | 25958136 |
whole-genome sequences of two rare human group c rotavirus strains isolated from two cases of acute gastroenteritis. | this is a report of the complete genomic sequences of two rare group c rotavirus strains rvc/sz94/chn/2011 and rvc/sz272/chn/2011, isolated from two cases of acute gastroenteritis in shenzhen, southern china, in 2011. these two strains display a close genetic relationship to 2007 chinese strain ynr001 and 2008 japanese strain bk0830. | 2015 | 26358598 |
phylogenetic analysis of human group c rotavirus in hospitalized children with gastroenteritis in belém, brazil. | group c rotavirus (rvc) is potentially an important pathogen associated with acute gastroenteritis (ag), especially in outbreaks. this study aims to detect and molecularly characterize rvc in hospitalized children with ag in belém, brazil. from may 2008 to april 2011, 279 stools were subjected to reverse-transcription polymerase chain reaction targeting vp7, vp6, vp4, and nsp4 genes. rvc positivity rate was 2.1% (6/279) and phylogenetic analysis of positive samples yields genotype g4-p[2]-i2-e2. ... | 2016 | 26369400 |
complete genome sequence of rotavirus group c isolated in south korea. | rotavirus group c is the major etiological agent associated with acute gastroenteritis in all human age groups. here, we report the complete genome sequence of human group c rotavirus (gpc-rv) isolated in south korea. | 2015 | 26404599 |
first genetic characterization of rotavirus c in russia. | rotaviruses c (rvc) cause sporadic cases and outbreaks of diarrhea in humans and animals worldwide. the aim of this study was to monitor rvc during a surveillance study of sporadic cases of viral gastroenteritis in the novosibirsk and omsk regions of russia from 2006 to 2011. a total of 2144 stool samples from children and adults hospitalized with acute gastroenteritis were tested for rvc by rt-pcr. sixteen rvc-positive stool samples were detected at a rate of 0.6% (13/2037) in children and 2.8% ... | 2016 | 26773827 |
detection of astrovirus, rotavirus c, and hepatitis e viral rna in adult and juvenile farmed mink (neovison vison). | mink astrovirus (miastv) is known to play a major role in mink pre-weaning diarrhea, and rotavirus and hepatitis e virus (hev) are both considered potentially zoonotic agents. these viruses are not monitored in commercial mink, and the role of these viral infections in mink health is not well understood. this study assessed the prevalence of mink astrovirus, rotavirus c, mink hev and swine hev in 527 pooled healthy adult female mink and mink kit fecal samples from 50 canadian mink farms in two s ... | 2018 | 29974054 |
kobuvirus detection in the critically endangered pygmy hog (porcula salvania), india. | pygmy hogs (porcula salvania) are the smallest and rarest wild suid. it is categorized as a critically endangered species as per the red list of the international union for conservation of nature. this study reports the first detection of a single-stranded rna virus species, aichivirus c, belonging to the genus kobuvirus (kobv) and the family picornaviridae, in pygmy hogs. kobv species are identified as a cause of acute gastroenteritis among children in india. as of now, there exists no report o ... | 2021 | 33827197 |