| contamination of bone marrow products with an actinomycete resembling microbacterium species and reinfusion into autologous stem cell and bone marrow transplant recipients. | bacterial contamination of bone marrow or peripheral blood stem cell transplant products typically occurs with skin flora or, rarely, gram-negative organisms. we describe a clonal outbreak of contamination in transplant products caused by contamination with an aerobic actinomycete that occurred at our institution during the summer of 2001. from 1 july through 12 september 2001, 73 peripheral blood or bone marrow stem cell products were obtained from 39 patients, and 34 products were found to be ... | 2003 | 12746790 |
| identifying the bacterial community on the surface of intralox belting in a meat boning room by culture-dependent and culture-independent 16s rdna sequence analysis. | we examined the bacterial community present on an intralox conveyor belt system in an operating lamb boning room by sequencing the 16s ribosomal dna (rdna) of bacteria extracted in the presence or absence of cultivation. rflp patterns for 16s rdna clone library and cultures were generated using haeiii and mspi restriction endonucleases. 16s rdna amplicons produced 8 distinct rflp pattern groups. rflp groups i-iv were represented in the clone library and rflp groups i and v-viii were represented ... | 2006 | 16488497 |
| sediment microbes of deep-sea bioherms on the northwest shelf of australia. | the northwest shelf of australia is a region with known petroleum reserves. recent geological investigations have revealed carbonate knolls postulated to be of biological origin. sediment microbial populations at three sites on the northwest shelf, pee shoal, mermaid reef, and scott reef, were investigated for the presence of hydrocarbon-degrading bacteria. from two sampling trips, 246 bacterial strains were purified, and 182 of these strains displayed preferential growth on hydrocarbon-selectiv ... | 2003 | 12739077 |
| the microbacteria ii. : the growth of microbacterium lacticum in a chemically defined medium. | | 1949 | 16561650 |
| diversity of 746 heterotrophic bacteria isolated from microbial mats from ten antarctic lakes. | microbial mats, growing in antarctic lakes constitute unique and very diverse habitats. in these mats microorganisms are confronted with extreme life conditions. we isolated 746 bacterial strains from mats collected from ten lakes in the dry valleys (lakes hoare and fryxell), the vestfold hills (lakes ace, druzhby, grace, highway, pendant, organic and watts) and the larsemann hills (lake reid), using heterotrophic growth conditions. these strains were investigated by fatty acid analysis, and by ... | 2002 | 12583721 |
| microbiology of acute otitis externa. | to isolate and characterize bacteria and fungi from acute otitis externa (aoe) and to obtain susceptibility profiles on each bacterial isolate. | 2002 | 12169893 |
| microbacterium aerolatum sp. nov., isolated from the air in the 'virgilkapelle' in vienna. | three rod-shaped, gram-positive strains were isolated from the air of the chapel 'virgilkapelle' in vienna. a representative of these three strains, strain v-73t, shared the highest 16s rdna sequence similarities with members of the genus microbacterium, in particular microbacterium foliorum, microbacterium testaceum, microbacterium esteraromaticum, microbacterium keratanolyticum and microbacterium arabinogalactanolyticum. the strains displayed almost identical biochemical and physiological char ... | 2002 | 12148633 |
| the hydantoin transport protein from microbacterium liquefaciens. | the gene hyup from microbacterium liquefaciens aj 3912 with an added his6 tag was cloned into the expression plasmid pttq18 in an escherichia coli host strain. the transformed e. coli showed transport of radioisotope-labeled 5-substituted hydantoins with apparent k(m) values in the micromolar range. this activity exhibited a ph optimum of 6.6 and was inhibited by dinitrophenol, indicating the requirement of energy for the transport system. 5-indolyl methyl hydantoin and 5-benzyl hydantoin were t ... | 2006 | 16621827 |
| catheter-related microbacterium bacteremia identified by 16s rrna gene sequencing. | we describe the application of 16s rrna gene sequencing in defining two cases of catheter-related microbacterium bacteremia. in the first case, a gram-positive bacillus was isolated from both the blood culture and central catheter tip of a 39-year-old woman with chronic myeloid leukemia. the api coryne system identified the isolate as 98.9% aureobacterium or corynebacterium aquaticum. in the second case, a gram-positive bacillus was recovered from five sets of blood cultures from both central ca ... | 2002 | 12089308 |
| rapid analysis of two food-borne microbial communities at the species level by fourier-transform infrared microspectroscopy. | the species composition of microbial communities in natural habitats may be extremely complex and therefore a quantitative analysis of the fraction each species contributes to the consortium has proven to be difficult. during recent years, the identification of bacterial pure cultures based on their infrared spectra has been established. fourier-transform infrared microspectroscopy now proceeds a step further and allows identification of microorganisms directly plated from community dilutions. i ... | 2006 | 16623742 |
| characterization of the novel hch-degrading strain, microbacterium sp. itrc1. | a gram-positive microbacterium sp. strain, itrc1, that was able to degrade the persistent and toxic hexachlorocyclohexane (hch) isomers was isolated and characterized. the itrc1 strain has the capacity to degrade all four major isomers of hch present in both liquid cultures and aged contaminated soil. dna fragments corresponding to the two initial genes involved in gamma-hch degradative pathway, encoding enzymes for gamma-pentachlorocyclohexene hydrolytic dehalogenase (linb) and a 2,5-dichloro-2 ... | 2006 | 16315057 |
| enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms. | most biofilms in their natural environments are likely to consist of consortia of species that influence each other in synergistic and antagonistic manners. however, few reports specifically address interactions within multispecies biofilms. in this study, 17 epiphytic bacterial strains, isolated from the surface of the marine alga ulva australis, were screened for synergistic interactions within biofilms when present together in different combinations. four isolates, microbacterium phyllosphaer ... | 2006 | 16751497 |
| biofilm formation characteristics of bacterial isolates retrieved from a reverse osmosis membrane. | high-quality water purification systems using reverse osmosis (ro) membrane separation have faced a major challenge related to biofilm formation on the membrane surface, or biofouling. to understand this issue, the biofilm formation characteristics of four bacterial isolates previously retrieved from an ro membrane treating potable water were investigated. biofilm formation of all four isolates occurred to different extents in microtiter plates and could be related to one or more cell properties ... | 2005 | 16245826 |
| coaggregation between acinetobacter johnsonii s35 and microbacterium esteraromaticum strains isolated from sewage activated sludge. | the extent and nature of intergeneric coaggregations among non-flocculating sludge bacteria were studied through examination of the coaggregation abilities of acinetobacter johnsonii s35 with two other strains of non-flocculating sludge bacteria (microbacterium esteraromaticum s38 and m. esteraromaticum s51). at first, the effect of electrolyte concentration as well as the addition of edta and proteases on coaggregation were studied. changes in electrolyte concentration had little effect on the ... | 2003 | 16233476 |
| microbacterium xylanilyticum sp. nov., a xylan-degrading bacterium isolated from a biofilm. | a novel xylan-degrading bacterium, s3-e(t), was isolated from the biofilm of a membrane bioreactor. the cells of this strain were gram-positive, non-motile, non-spore-forming rods, produced primary branches and formed yellow colonies on nutrient agar. the strain had chemotaxonomic markers that were consistent with classification in the genus microbacterium, i.e. mk-12, mk-11 and mk-13 as the major menaquinones, predominant iso- and anteiso-branched cellular fatty acids, glucose and galactose as ... | 2005 | 16166712 |
| [bacterial conversion of sulfur-and phosphorous-compounds and microbial diversity in sediments from a near-shore marine-cultural region]. | the h2s-producing bacteria and the phosphorous-cycling bacteria in sediments from near-shore marine-cultural region were investigated. results indicate that the bacterial h2s production in aerobic condition is the dominating process to produce h2s in the sediment of cultural pond. the total counts of h2s-producing bacteria utilizing cysteine and na2s2o3 were 1.6 x 10(6) and 4.35 x 10(3) cells g(-1) respectively. the counts of sulfate-reducing bacteria in the sediments were very little, only 2.5 ... | 2005 | 16004320 |
| biodesulfurization of dibenzothiophene and other organic sulfur compounds by a newly isolated microbacterium strain zd-m2. | a new bacterial strain capable of selectively desulfurizing dibenzothiphene (dbt) was isolated from sludge. this organism, designated zd-m2, clustered most closely with members of the genus microbacterium, as determined by 16s rrna gene sequence analysis. metabolites produced by dbt desulfurization were identified by gc-ms, and two sulfur-free products, 2-methoxybiphenyl (2-mbp) and biphenyl, were detected in addition to 2-hydroxybiphenyl (2-hbp). this strain can also totally degrade 4,6-dimethy ... | 2005 | 15927746 |
| purification and properties of a new type of protease produced by microbacterium liquefaciens. | a bacterium, identified as microbacterium liquefaciens mim-cg-9535-i, was isolated from a soil sample taken from the industrial site of a gelatin manufacturer. a new type of protease, which restrictively decomposes gelatin at one or two positions, was purified from the bacterial culture. the molecular mass of the purified enzyme was 21 kda by sds-polyacrylamide gel electrophoresis. the purified enzyme specifically degraded the alpha-chain of gelatin with a molecular weight of 100 kda into two pe ... | 2005 | 15914910 |
| genome characterization, analysis of virulence and transformation of microbacterium nematophilum, a coryneform pathogen of the nematode caenorhabditis elegans. | a coryneform bacterium designated microbacterium nematophilum has previously been reported to act as a pathogen for caenorhabditis elegans. this bacterium is able to colonize the rectum of infected worms and cause localized swelling, constipation and slowed growth. additional isolates and analysis of this bacterium are described here. tests of pathogenicity on other caenorhabditis nematodes show that m. nematophilum infection is lethal to most species in the genus, in contrast to its relatively ... | 2006 | 17010162 |
| reduction of cr(vi) by immobilized cells of desulfovibrio vulgaris ncimb 8303 and microbacterium sp. ncimb 13776. | hexavalent chromium, a carcinogen and mutagen, can be reduced to cr(iii) by desulfovibrio vulgaris ncimb 8303 and microbacterium sp. ncimb 13776. this study examined cr(vi) reduction by immobilized cells of the two strains in a common solution matrix using various entrapment matrices. chitosan and pva-borate beads did not retain integrity and supported low or no reduction of cr(vi) by the cells. a commercial preparation (lentikats) was stable but also did not support cr(vi) reduction. k-carragee ... | 2005 | 15818565 |
| purification and properties of a keratinolytic metalloprotease from microbacterium sp. | this study was developed to purify and to characterize a keratinolytic protease from the bacterium microbacterium sp. strain kr10. | 2006 | 17105556 |
| purification and characterization of hydantoin racemase from microbacterium liquefaciens aj 3912. | a hydantoin racemase that catalyzed the racemization of 5-benzyl-hydantoin was detected in a cell-free extract of microbacterium liquefaciens aj 3912, a bacterial strain known to produce l-amino acids from their corresponding dl-5-substituted-hydantoins. this hydantoin racemase was purified 658-fold to electrophoretic homogeneity by serial chromatography. the n-terminal amino acid sequence of the enzyme showed homology with known hydantoin racemases from other microorganisms. the apparent molecu ... | 2005 | 15784981 |
| molecular diversity studies of bacterial communities of oil polluted microbial mats from the etang de berre (france). | the biodiversity of microbial mats inhabiting the oil-contaminated lagoon etang de berre was determined by molecular approaches. the fingerprint of denaturing gradient gel electrophoresis (dgge) and automatic ribosomal intergenic spacer analysis (arisa) of mats exposed to different pollution levels showed specific microbial communities for each site but similar diversity richness. species composition of the mats were compared by constructing 16s rrna libraries. amplified rdna restriction analysi ... | 2006 | 17117996 |
| microbacterium natoriense sp. nov., a novel d-aminoacylase-producing bacterium isolated from soil in natori, japan. | a rod-shaped, gram-positive bacterium, strain tnjl143-2(t), having n-acyl-d-amino acid amidohydrolase (d-aminoacylase) activity, was isolated from a soil sample from natori, japan. it was a non-spore-forming, strictly aerobic bacterium without motility, showing a temperature optimum for growth of 30 degrees c and a ph optimum for growth of 5-7. the 16s rrna gene sequence of the strain showed the highest similarities to members of the genus microbacterium, in particular, microbacterium aerolatum, ... | 2005 | 15774640 |
| characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria. | this study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (eps). | 2007 | 17184342 |
| chilling and cultivar type affect the diversity of bacterial endophytes colonizing sweet pepper (capsicum anuum l.). | a climate chamber experiment was conducted to assay the effect of low temperatures (chilling) on the diversity of bacteria colonizing the endospheres of two thermophilic sweet pepper (capsicum anuum l.) cultivars, milder spiral and ziegenhorn bello. structural diversity was analyzed by 16s rrna-based terminal restriction fragment length polymorphism (t-rflp) analysis and by the generation of 16s rrna gene libraries to determine dominant community members in t-rflp profiles. cultivable community ... | 2006 | 17215894 |
| a new microbacterium species isolated from the blood of a patient with fever: microbacterium pyrexiae sp. nov. | a gram-positive bacterium, smc-a8265(t), which was isolated from the blood of a patient with fever but could not be identified by a conventional microbiologic method, was finally characterized by performing phenotypic and genotypic analyses. 16s rrna gene sequence analysis revealed that the strain smc-a8265(t) belonged to the genus microbacterium, but it did not correspond to any of the previously described microbacterium spp. biochemical tests and cellular fatty acid composition analysis also c ... | 2007 | 17240108 |
| identification of diazotrophs in the culturable bacterial community associated with roots of lasiurus sindicus, a perennial grass of thar desert, india. | lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the thar desert of rajasthan, india. analysis of 16s rrna coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of l. sindicus, showed predominance of gram-negative over gram-positive bacteria. according to comparative sequence analysis of 16s rdna sequence data, gram-positive bacteria with low gc content (staphylococcus warneri and bacillus ... | 2007 | 17264993 |
| heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in ni-rich serpentine soil and in the rhizosphere of alyssum murale. | forty-six bacterial cultures, including one culture collection strain, thirty from the rhizosphere of alyssum murale and fifteen from ni-rich soil, were tested for their ability to tolerate arsenate, cadmium, chromium, zinc, mercury, lead, cobalt, copper, and nickel in their growth medium. the resistance patterns, expressed as minimum inhibitory concentrations, for all cultures to the nine different metal ions were surveyed by using the agar dilution method. a large number of the cultures were r ... | 2007 | 17276484 |
| 17beta-estradiol-degrading bacteria isolated from activated sludge. | fourteen phylogenetically diverse 17beta-estradiol-degrading bacteria (strains kc1-14) were isolated from activated sludge of a wastewater treatment plant. these isolates widely distributed among eight different genera--aminobacter (strains kc6 and kc7), brevundimonas (strain kc12), escherichia (strain kc13), flavobacterium (strain kc1), microbacterium (strain kc5), nocardioides (strain kc3), rhodococcus (strain kc4), and sphingomonas (strains kc8-kc11 and kc14)--of three phyla: proteobacteria, ... | 2007 | 17310711 |
| caenorhabditis elegans mutants resistant to attachment of yersinia biofilms. | the detailed composition and structure of the caenorhabditis elegans surface are unknown. previous genetic studies used antibody or lectin binding to identify srf genes that play roles in surface determination. infection by microbacterium nematophilum identified bus (bacterially unswollen) genes that also affect surface characteristics. we report that biofilms produced by yersinia pestis and y. pseudotuberculosis, which bind the c. elegans surface predominantly on the head, can be used to identi ... | 2007 | 17339204 |
| [evaluation of api coryne system, version 2.0, for diphteroid gram-positive rods identification with clinical relevance]. | the ability of the api coryne system, version 2.0, to identify 178 strains of gram-positive rods was evaluated. seventy eight isolates belonged to genus corynebacterium and one hundred to related genera, all strains were isolated from clinical samples at the laboratory of bacteriology, hospital de clínicas josé de san martin (uba) between 1995 and 2004. the isolates were identified according to von graevenitz and funke's scheme. one hundred and sixty two out of 178 strains (91%) were correctly i ... | 2006 | 17370571 |
| microbacterium oleivorans sp. nov. and microbacterium hydrocarbonoxydans sp. nov., novel crude-oil-degrading gram-positive bacteria. | a taxonomic study of two crude-oil-degrading, gram-positive bacterial strains, designated bas69(t) and bnp48(t), revealed that they represent two novel microbacterium species. 16s rrna gene sequence similarity to their closest phylogenetic neighbours was 98.5 % for bas69(t) (microbacterium paraoxydans dsm 15019(t) and microbacterium saperdae dsm 20169(t)) and 99 % for bnp48(t) (microbacterium luteolum dsm 20143(t)). levels of dna-dna relatedness to the closest phylogenetic neighbours of both str ... | 2005 | 15774639 |
| metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake. | in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ... | 2007 | 17406774 |
| [reaction of microorganisms to the digestive fluid of the earthworms]. | the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ... | 2007 | 17410875 |
| culture-independent analysis of bacterial diversity in a child-care facility. | child-care facilities appear to provide daily opportunities for exposure and transmission of bacteria and viruses. however, almost nothing is known about the diversity of microbial contamination in daycare facilities or its public health implications. recent culture-independent molecular studies of bacterial diversity in indoor environments have revealed an astonishing diversity of microorganisms, including opportunistic pathogens and many uncultured bacteria. in this study, we used culture and ... | 2007 | 17411442 |
| isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. cv. br29 of bangladesh. | a total of 30 bacteria were isolated from the rhizoplane of rice cv. br29 cultivated in mymensingh, bangladesh and from the seedlings obtained from surface-sterilized seeds of br29. upon screening, 6 isolates showed varying levels of phosphate solubilizing activity in both agar plate and broth assays using national botanical research institute's phosphate medium. the bacterial isolates were identified based on their phenotypic and 16s rrna genes sequencing data as acinetobacter sp. br-12, klebsi ... | 2007 | 17425114 |
| microbacterium halotolerans sp. nov., isolated from a saline soil in the west of china. | a gram-positive short rod isolated from a saline soil in china was characterized using a polyphasic approach. this actinobacterium grew over a wide salinity range [0-15 % nacl, 0-20 % kcl and 0-30 % (mgcl2).6h2o (w/v); optimum concentrations for growth were 5 % nacl, 5-10 % kcl, 10 % (mgcl2).6h2o]. the optimum growth temperature and ph were 28-30 degrees c and 7.0-8.0, respectively. chemotaxonomic features (peptidoglycan-type b2beta with glycolyl residues; major menaquinones mk-10 and mk-11; pre ... | 2005 | 15653854 |
| effects of ph amendment on metal working fluid wastewater biological treatment using a defined bacterial consortium. | the aim of this study was to determine whether ph amendment of a highly alkaline metal working fluid (mwf) wastewater would improve biological treatment in a bioreactor system following introduction of a bacterial inoculum (comprised of the following strains: agrobacterium radiobacter, comamonas testosteroni, methylobacterium mesophilicum, microbacterium esteraromaticum, and microbacterium saperdae). the ph values tested were 6, 7, 8, and 9. three replicate batch mode bioreactors inoculated with ... | 2005 | 15625673 |
| isolation and characterization of a xanthan-degrading microbacterium sp. strain xt11 from garden soil. | isolation and characterization of the xanthan-degrading microbacterium sp. xt11. | 2007 | 17448171 |
| synthesis, solution behavior, thermal stability, and biological activity of an fe(iii) complex of an artificial siderophore with intramolecular hydrogen bonding networks. | previously, an artificial siderophore complex, the iron(iii) complex with tris[2-[(n-acetyl-n-hydroxy)glycylamino]ethyl]amine (tage), was constructed in order to understand the effect of intramolecular hydrogen bonding interaction on the siderophore function, and its structural characterization in the solid state was reported (inorg. chem. 2001, 40, 190). in this paper, the solution behavior of the m(iii)-tage (m = fe, ga) system has been investigated using (1)h nmr, uv-vis, and fab mass spectro ... | 2004 | 15606204 |
| antimicrobial properties of two purified skin peptides from the mink frog (rana septentrionalis) against bacteria isolated from the natural habitat. | numerous peptides exhibiting antimicrobial properties have been isolated from the skins of many amphibian species. these peptides offer an innate chemical defense system against various microbial agents that exist in the amphibian's environment. amphibian skin peptides are typically tested for antimicrobial activity against microbial strains that are pathogenic to humans, but not on potential pathogenic or opportunistic bacteria that exist in the organism's habitat. two peptides, a brevinin-2-re ... | 2007 | 17499556 |
| effect of levan's branching structure on antitumor activity. | levan produced from microbacterium laevaniformans kctc 9732 (m-levan) was isolated and treated with an inulinase to modify its branching structure. the chemical structures of levans were characterized, and the modified levans were applied on animal tumor cells to evaluate their antitumor activity. the gc-ms analysis indicated that beta-(2,1)-linked branches of m-levan were specifically hydrolyzed. as the ratio of applied inulinase to levan increased, the branching degree decreased proportionally ... | 2004 | 15225991 |
| papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host-endophyte interaction in vitro and in vivo. | fourteen distinct bacterial clones were isolated from surface-sterilized shoot tips (approximately 1 cm) of papaya (carica papaya l. 'surya') planted on murashige and skoog (ms)-based papaya culture medium (23/50 nos.) during the 2-4 week period following in vitro culturing. these isolates were ascribed to six gram-negative genera, namely pantoea (p. ananatis), enterobacter (e. cloacae), brevundimonas (b. aurantiaca), sphingomonas, methylobacterium (m. rhodesianum), and agrobacterium (a. tumefac ... | 2007 | 17538647 |
| microbacterium koreense sp. nov., from sea water in the south sea of korea. | microbacterium strains js53-2t and js53-5 were isolated from sea water in the south sea of korea and subjected to phenotypic, chemotaxonomic and genetic characterization. the cells were found to be gram-positive. these strains contained mk-11 and mk-12 as the main respiratory quinones and anteiso-c15:0, anteiso-c17:0 and iso-c16:0 as the major cellular fatty acids. the dna g+c content was 68 mol%. the cell-wall sugars of the isolates were galactose and xylose, and the diamino acid in the cell-wa ... | 2006 | 16449451 |
| effects of ultraviolet radiation on the gram-positive marine bacterium microbacterium maritypicum. | although extensive information is available on the effect ultraviolet (uv) radiation has on gram-negative marine bacteria, there is a scarcity of data concerning uv radiation and gram-positive marine bacteria. the focus of this paper is on microbacterium maritypicum, with the gram-negative vibrio natriegens being used as a standard of comparison. m. maritypicum exhibited growth over a nacl range of 0-1000 mm: , with optimum growth occurring between 0 and 400 mm: nacl. in contrast, v. natriegens ... | 2007 | 17551790 |
| molecular cloning, expression and characterization of a chitosanase from microbacterium sp. | a gene encoding a chitosanase (mschito) was cloned from microbacterium sp. ou01. the orf consists of 801 bp which encoded a polypeptide of 266 amino acid residues. the deduced amino acid sequence shows 98% identity to that of the chitosanase reported in pseudomonas sp. a-01. in addition, the fusion protein containing mschito was expressed in e. coli and purified using ni-nta affinity chromatography. the purified rmschito protein degraded the chitosan (the degree of deacetylation of 99%) and prod ... | 2007 | 17563859 |
| improved production of bioactive glucosylmannosyl-glycerolipid by sponge-associated microbacterium species. | the marine microbacterium species hp2 (dsm 12583), isolated from the sponge halichondria panicea, is able to produce a glucosylmannosyl-glycerolipid when grown on a complex medium with glucose. optimizing the carbon sources in shake flask experiments has shown that glycerol affords the highest specific glycoglycerolipid production. the product yield approached 300 mg/l or 25 mg/g biomass upon scaling up in a 40-l bioreactor volume. the native diglycosyl-glycerolipid ggl.2 strongly inhibited grow ... | 2004 | 15085410 |
| purification and characterization of a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents, from dimethylsulfoxide-tolerant brachybacterium sp. strain lb25. | a bacterium that secretes maltooligosaccharide-forming amylase in a medium containing 12.5% (vol/vol) dimethylsulfoxide (dmso) was isolated and identified as brachybacterium sp. strain lb25. the amylase of the strain was purified from the culture supernatant, and its molecular mass was 60 kda. the enzyme was stable at ph 7.0-8.5 and active at ph 6.0-7.5. the optimum temperature at ph 7.0 was 35 degrees c in the presence of 5 mm cacl(2). the enzyme hydrolyzed starch to produce maltotriose primari ... | 2007 | 17619813 |
| the crystal structure of a (-) gamma-lactamase from an aureobacterium species reveals a tetrahedral intermediate in the active site. | the structure of the recombinant (-) gamma-lactamase from an aureobacterium species has been solved at 1.73a resolution in the cubic space group f23 with unit cell parameters a=b=c=240.6a. the trimeric enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor free haloperoxidases. the structure has been solved in complex with a covalently bound ligand originating from the host cell and also in the unligated form. the associated density in the former structure has been interprete ... | 2004 | 15081810 |
| isolation of bacterial endophytes from germinated maize kernels. | the germination of surface-sterilized maize kernels under aseptic conditions proved to be a suitable method for isolation of kernel-associated bacterial endophytes. bacterial strains identified by partial 16s rrna gene sequencing as pantoea sp., microbacterium sp., frigoribacterium sp., bacillus sp., paenibacillus sp., and sphingomonas sp. were isolated from kernels of 4 different maize cultivars. genus pantoea was associated with a specific maize cultivar. the kernels of this cultivar were ofte ... | 2007 | 17668041 |
| interstitial pulmonary inflammation due to microbacterium sp. after heart transplantation. | a coryneform bacterium was isolated from the bronchoalveolar aspirate of a patient with interstitial pulmonary inflammation. commercial systems identified the isolate as corynebacterium sp. or aureobacterium sp./corynebacterium aquaticum, but 16s rrna gene analysis unequivocally attributed it to the genus microbacterium. this represents the first documented case of microbacterium pulmonary infection. | 2006 | 16476799 |
| microbacterium paludicola sp. nov., a novel xylanolytic bacterium isolated from swamp forest. | a xylanolytic bacterium, us15t, was isolated from swamp forest soil in ulsan, korea. the cells of the novel strain were gram-positive, non-motile, short-rod-shaped and showed chemotaxonomic properties that were consistent with its classification in the genus microbacterium. chemotaxonomic results showed mk-12 and mk-11 as major menaquinones, predominating iso- and anteiso-branched cellular fatty acids, glucose, galactose and mannose as cell-wall sugars, peptidoglycan-type b2beta with glycolyl re ... | 2006 | 16514023 |
| production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses. | twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ... | 2007 | 17701035 |
| planktonic replication is essential for biofilm formation by legionella pneumophila in a complex medium under static and dynamic flow conditions. | legionella pneumophila persists for a long time in aquatic habitats, where the bacteria associate with biofilms and replicate within protozoan predators. while l. pneumophila serves as a paradigm for intracellular growth within protozoa, it is less clear whether the bacteria form or replicate within biofilms in the absence of protozoa. in this study, we analyzed surface adherence of and biofilm formation by l. pneumophila in a rich medium that supported axenic replication. biofilm formation by t ... | 2006 | 16597995 |
| [effect of pentachlorophenol (pcp) on bacterial community of an aerobic granular sludge estimated by t-rflp]. | effect of pentachlorophenol (pcp) on cod and ammonium removal rates was investigated, and also changes of bacterial community within aerobic granular sludge were monitored by terminal restriction fragment length polymorphism (t-rflp) technique. compared with the removal rate of cod, the rate of ammonium removal is more sensitive to pcp. as the ammonium and cod removal rates decreased consistently, the bacterial genera within the aerobic granular sludge also decrease with the increase of pcp conc ... | 2006 | 16768009 |
| genomic clusters, putative pathogen recognition molecules, and antimicrobial genes are induced by infection of c. elegans with m. nematophilum. | the interaction between the nematode caenorhabditis elegans and a gram-positive bacterial pathogen, microbacterium nematophilum, provides a model for an innate immune response in nematodes. this pathogen adheres to the rectal and post-anal cuticle of the worm, causing slowed growth, constipation, and a defensive swelling response of rectal hypodermal cells. to explore the genomic responses that the worm activates after pathogenic attack we used microarray analysis of transcriptional changes indu ... | 2006 | 16809667 |
| vitamin requirements of microbacterium lacticum orla-jensen. | | 1947 | 17797253 |
| diversity of microorganisms in decaying maize stalks revealed by a molecular method. | microbial diversity in decaying maize stalk was characterized by constructing and analyzing rrna gene clone library. total 47 otus were obtained from 82 bacterial clones, including proteobacteria (64.6%), actinobacteria (30.5%), bacteroidetes (2.4%) and firmicutes (2.4%). most proteobacterial clones were members of rhizobium, pseudomonas and stenotrophomonas. eighty-four percent of actinobacteria was related to microbacterium. only 14 otus were identified from 124 fungal clones, including ascomy ... | 2007 | 17846593 |
| purification and characterization of two types of chitosanase from a microbacterium sp. | two extracellular chitosanases (chix and chin) were extracted from microbacterium sp. ou01 with mr values of 81 kda (chix) and 30 kda (chin). chin was optimally active at ph 6.2 and 50 degrees c and chix at ph 6.6 and 60 degrees c (assayed over 15 min). both the activities increased with the degree of deacetylation (dda) of chitosan. chin hydrolyzed oligomers of glucosamine (glcn) larger than chitopentaose, and chitosan with 62-100% dda; but chix acted on chitosan and released glcn. hydrolysis o ... | 2006 | 16820974 |
| substrate induction and statistical optimization for the production of chitosanase from microbacterium sp. ou01. | the chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by microbacterium sp. ou01 in shake-flask. a significant influence of (nh(4))(2)so(4), mgso(4).7h(2)o and initial ph on chitosanase production was noted with plackett-burman design. it was then revealed with the method of steepest ascent and response surface methodology (rsm) that 19.0g/l (nh(4))(2)so(4), 1.3g/l mgso(4) and an initial ph of 2.0 were optim ... | 2007 | 16930999 |
| microbacterium deminutum sp. nov., microbacterium pumilum sp. nov. and microbacterium aoyamense sp. nov. | three novel bacterial strains were isolated from a soil sample collected in japan by culture on a gpm agar plate supplemented with superoxide dismutase and catalase. the strains were gram-positive, catalase-positive, non-motile bacteria with l-ornithine as a diagnostic diamino acid of the peptidoglycan. the acyl type of the peptidoglycan was n-glycolyl. the major menaquinones were mk-12, 13 and 14. mycolic acids were not detected. g+c contents of the dna were in the range 69-71 mol%. comparative ... | 2006 | 16957107 |
| culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits. | during cold storage after milk collection, psychrotrophic bacterial populations dominate the microflora, and their extracellular enzymes, mainly proteases and lipases, contribute to the spoilage of dairy products. the diversity, dynamics, and enzymatic traits of culturable psychrotrophs in raw milk from four farms were investigated over a 10-month period. about 20% of the isolates were found to be novel species, indicating that there is still much to be learned about culturable psychrotrophs in ... | 2007 | 17890340 |
| diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of burkholderia cepacia complex isolates. | bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in brazil. endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16s rrna gene sequence analysis revealed that the select ... | 2007 | 17905875 |
| growth and colour development of some surface ripening bacteria with debaryomyces hansenii on aseptic cheese curd. | the growth of five bacteria isolated from red-smear cheeses, brevibacterium aurantiacum, corynebacterium casei, corynebacterium variabile, microbacterium gubbeenense and staphylococcus saprophyticus in mixed cultures with debaryomyces hansenii on aseptic model cheese curd at 10 and 14 degrees c was investigated. at both temperatures, c. casei and micro. gubbeenense had a longer lag phase than c. variabile, brevi. aurantiacum and staph. saprophyticus. in all cultures, lactose was utilised first a ... | 2006 | 16978429 |
| growth characteristics of brevibacterium, corynebacterium, microbacterium, and staphylococcus spp. isolated from surface-ripened cheese. | the growth characteristics of five bacteria, brevibacterium aurantiacum 1-16-58, corynebacterium casei dpc 5298(t), corynebacterium variabile dpc 5310, microbacterium gubbeenense dpc 5286(t), and staphylococcus saprophyticus 4e61, all of which were isolated from the surface of smear cheese, were studied in complex and chemically defined media. all of the coryneforms, except m. gubbeenense, grew in 12% salt, while b. aurantiacum and s. saprophyticus grew in 15% salt. all five bacteria assimilated ... | 2007 | 17921266 |
| degradation of environmental endocrine disruptor di-2-ethylhexyl phthalate by a newly discovered bacterium, microbacterium sp. strain cq0110y. | in this study di-2-ethylhexyl phthalate (dehp)-degradation strain cq0110y was isolated from activated sludge. according to the biophysical/biochemical characteristics and analysis of 16s rdna, the strain was identified as microbacterium sp. the results of this study showed the optimal ph value and optimal temperature which influenced the degradation rate in wastewater: ph 6.5-7.5, 25-35 degrees c. kinetics of degradation reaction had been performed at different initial concentrations and differe ... | 2007 | 17089119 |
| interactions with microbial pathogens. | a wide variety of bacterial pathogens, as well as several fungi, kill c. elegans or produce non-lethal disease symptoms. this allows the nematode to be used as a simple, tractable model host for infectious disease. human pathogens that affect c. elegans include gram-negative bacteria of genera burkholderia, pseudomonas, salmonella, serratia and yersinia; gram-positive bacteria enterococcus, staphylococcus and streptococcus; and the fungus cryptococcus neoformans. microbes that are not pathogenic ... | 2005 | 18050390 |
| degradation of phenanthrene on plant leaves by phyllosphere bacteria. | the activity of phyllosphere bacteria in the degradation of phenanthrene was investigated as a mechanism for the removal of atmospheric phenanthrene after its deposition on plant leaves. initially, leaf samples of six plant species were collected from two roadsides in bangkok to determine the presence of phenanthrene-degrading bacteria. the numbers of phenanthrene-degrading phyllosphere bacteria were varied and ranged from 3.5 x 10(4) to 1.95 x 10(7) cfu/g, in which the highest number was found ... | 2007 | 18057816 |
| activities and survival of endophytic bacteria in white clover (trifolium repens l.). | in this study, the genera, abundance, and activities of endophytic bacteria in field-grown white clover (trifolium repens) and the fate of introduced antibiotic-tolerant bacteria in white clover tissues were investigated. pseudomonas, pantoea, and corynebacterium were the most frequently isolated endophytic bacteria genera, whereas xanthomonas, microbacterium, and cellulomonas occurred less frequently. the average bacterial populations in stolons and roots were approximately 100,000 colony-formi ... | 2006 | 17110977 |
| [microbial diversity in scorpion intestine (buthus martensii karsch)]. | scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ... | 2007 | 18062268 |
| effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation. | spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ... | 2008 | 18083857 |
| the growth of microbacterium lacticum in a chemically defined medium. | | 1949 | 18108881 |
| infections caused by coryneform bacteria in pediatric oncology patients. | invasive infections caused by coryneform bacteria are uncommon but have been reported with increasing frequency in recent decades, especially in immunocompromised persons. because pediatric experience is limited, we examined the epidemiology and clinical characteristics of these infections in children undergoing cancer therapy. | 2008 | 18174873 |
| a novel recombinant system for functional expression of myonecrotic snake phospholipase a(2) in escherichia coli using a new fusion affinity tag. | a novel recombinant expression system in escherichia coli was developed using conger eel galectin, namely, congerin ii, as an affinity tag. this system was applied for the functional expression of myotoxic lysine-49-phospholipase a(2) ([lys(49)]pla(2)), termed bpii and obtained from protobothrops flavoviridis (pf) venom. recombinant pf bpii fused with a congerin tag has been successfully expressed as a soluble fraction and showed better quantitative yield when folded correctly. the solubility of ... | 2008 | 18207418 |
| microbacterium ginsengisoli sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field. | strain gsoil 259(t), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the republic of korea and characterized in order to determine its taxonomic position. cells were gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16s rrna gene sequence analysis revealed that strain gsoil 259(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens ifo 3750(t) (98.5 %) and microbacterium imperiale ifo 1261 ... | 2008 | 18218944 |
| virulence of plant pathogenic bacteria attenuated by degradation of fatty acid cell-to-cell signaling factors. | diffusible signal factor (dsf) is a fatty acid signal molecule involved in regulation of virulence in several xanthomonas species as well as xylella fastidiosa. in this study, we identified a variety of bacteria that could disrupt dsf-mediated induction of virulence factors in xanthomonas campestris pv. campestris. while many bacteria had the ability to degrade dsf, several bacterial strains belonging to genera bacillus, paenibacillus, microbacterium, staphylococcus, and pseudomonas were identif ... | 2008 | 18257682 |
| assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches. | microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ... | 2008 | 18280146 |
| mos1 mutagenesis reveals a diversity of mechanisms affecting response of caenorhabditis elegans to the bacterial pathogen microbacterium nematophilum. | a specific host-pathogen interaction exists between caenorhabditis elegans and the gram-positive bacterium microbacterium nematophilum. this bacterium is able to colonize the rectum of susceptible worms and induces a defensive tail-swelling response in the host. previous mutant screens have identified multiple loci that affect this interaction. some of these loci correspond to known genes, but many bus genes [those with a bacterially unswollen (bus) mutant phenotype] have yet to be cloned. we em ... | 2007 | 17151260 |
| microbacterium isolates from the vicinity of a radioactive waste depository and their interactions with uranium. | three oligotrophic bacterial strains were cultured from the ground water of the deep-well monitoring site s15 of the siberian radioactive waste depository tomsk-7, russia. they were affiliated with actinobacteria from the genus microbacterium. the almost fully sequenced 16s rrna genes of two of the isolates, s15-m2 and s15-m5, were identical to those of cultured representatives of the species microbacterium oxydans. the third isolate, s15-m4, shared 99.8% of 16s rrna gene identity with them. the ... | 2007 | 17381522 |
| characterization of a cryptic plasmid from a greenland ice core arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high g+c gram-positive recipients. | over 60 greenland glacial isolates were screened for plasmids and antibiotic resistance/sensitivity as the first step in establishing a genetic system. sequence analysis of a small, cryptic, 1,950 bp plasmid, p54, from isolate gic54, related to arthrobacter agilis, showed a region similar to that found in theta replicating rhodococcus plasmids. a 6,002 bp shuttle vector, psvj21, was constructed by ligating p54 and puc18 and inserting a chloramphenicol acetyl transferase (cat) cassette conferring ... | 2008 | 18335166 |
| phylogenetic and enzymatic diversity of deep subseafloor aerobic microorganisms in organics- and methane-rich sediments off shimokita peninsula. | "a meta-enzyme approach" is proposed as an ecological enzymatic method to explore the potential functions of microbial communities in extreme environments such as the deep marine subsurface. we evaluated a variety of extra-cellular enzyme activities of sediment slurries and isolates from a deep subseafloor sediment core. using the new deep-sea drilling vessel "chikyu", we obtained 365 m of core sediments that contained approximately 2% organic matter and considerable amounts of methane from offs ... | 2008 | 18368287 |
| genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants. | the genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in cuba was analysed by two primers-ramdom amplified polymorphic dna fingerprinting (tp-rapd) and 16s rrna gene sequencing. the strains were distributed into 17 groups on the basis of their tp-rapd patterns, and a representative strain from each group was subjected to 16s rrna gene sequencing. analysis of these sequences showed that the is ... | 2008 | 18383223 |
| chemostat selection of a bacterial community able to degrade s-triazinic compounds: continuous simazine biodegradation in a multi-stage packed bed biofilm reactor. | using a successive transfer method on mineral salt medium containing simazine, a microbial community enriched with microorganisms able to grow on simazine was obtained. afterwards, using a continuous enrichment culture procedure, a bacterial community able to degrade simazine from an herbicide formulation was isolated from a chemostat. the continuous selector, fed with a mineral salt medium containing simazine and adjuvants present in the commercial herbicide formulation, was maintained in opera ... | 2008 | 18392868 |
| microbacterium terricolae sp. nov., isolated from soil in japan. | the taxonomic positions of two novel strains isolated from a soil sample collected in japan using glucose-peptone-meat extract (gpm) agar plates supplemented with superoxide dismutase or superoxide dismutase plus catalase were investigated based on the results of chemotaxonomic, phenotypic and genotypic characteristics. strains were gram-positive, catalase-positive, non-motile bacteria with l-ornithine as a diagnostic diamino acid of the peptidoglycan. the acyl type of the peptidoglycan was n-gl ... | 2007 | 17429156 |
| identification of cis-regulatory elements from the c. elegans t-box gene mab-9 reveals a novel role for mab-9 in hypodermal function. | we have identified conserved non-coding elements (cnes) in the regulatory region of caenorhabditis elegans and caenorhabditis briggsae mab-9, a t-box gene known to be important for cell fate specification in the developing c. elegans hindgut. two adjacent cnes (a region 78 bp in length) are both necessary and sufficient to drive reporter gene expression in posterior hypodermal cells. the failure of a genomic mab-9::gfp construct lacking this region to express in posterior hypodermis correlates w ... | 2008 | 18402933 |
| stability of the biodiversity of the surface consortia of gubbeen, a red-smear cheese. | a total of 1,052 bacteria and 828 yeasts were isolated from the surface flora of 6 batches of gubbeen cheese made in 1996-1997 and 2002-2003. stability of the microflora was evaluated over time and also during ripening at 4, 10, and 16 d (batches 4, 5, and 6) or at 4, 16, 23, and 37 d (batches 1, 2, and 3). bacteria were identified using pulsed-field gel electrophoresis, repetitive extragenic palindromic-pcr, and 16s rrna gene sequencing, and yeasts were identified by fourier transform infrared ... | 2007 | 17430918 |
| microbacterium aquimaris sp. nov., isolated from seawater. | strains js54-2(t) and js63-1 were isolated from seawater at jeju, korea. cells of these strains were gram-positive, non-motile, non-spore-forming, short rods and formed yellow-pigmented colonies on tryptic soy agar. the strains had chemotaxonomic markers that were consistent with their classification in the genus microbacterium, i.e. mk-11, mk-12 and mk-10 as the major menaquinones, fatty acids that were predominantly iso- and anteiso-branched, galactose and glucose as the cell-wall sugars, pept ... | 2008 | 18599704 |
| microbacterium luticocti sp. nov., isolated from sewage sludge compost. | strain sc-087b(t), isolated from sewage sludge compost during a study of bacterial diversity in composts, was characterized. the isolate was a gram-positive, short rod that was motile, catalase- and oxidase-negative and able to grow at 27-45 degrees c, ph 5.5-9.7 and in up to 10 % nacl. the peptidoglycan was of the b2beta type, containing the characteristic amino acids ornithine, homoserine and hydroxyglutamic acid. the muramic acid residues of the peptidoglycan were partially glycolylated. the ... | 2008 | 18599719 |
| probing the critical residues for intramolecular fructosyl transfer reaction of a levan fructotransferase. | levan fructotransferase (lftase) preferentially catalyzes the transfructosylation reaction in addition to levan hydrolysis, whereas other levan-degrading enzymes hydrolyze levan into a levan-oligosaccharide and fructose. based on sequence comparisons and enzymatic properties, the fructosyl transfer activity of lftase is proposed to have evolved from levanase. in order to probe the residues that are critical to the intramolecular fructosyl transfer reaction of the microbacterium sp. al-210 lftase ... | 2008 | 18600048 |
| treatment of bromoamine acid wastewater using combined process of micro-electrolysis and biological aerobic filter. | the wastewater originated from the production of bromoamine acid was treated in a sequential system of micro-electrolysis (me) and biological aerobic filter (baf). decolorization and cod(cr) removal rate of the proposed system was investigated with full consideration of the influence of two major controlling factors such as organic loading rate (olr) and hydraulic retention time (hrt). the removal rate of cod(cr) was 81.2% and that of chrominance could be up to 96.6% at an olr of 0.56 kg m(-3)d( ... | 2009 | 18621476 |
| identities of arthrobacter spp. and arthrobacter-like bacteria encountered in human clinical specimens. | after the initial description of arthrobacter spp. isolated from clinical specimens in the mid-1990s, very few further reports on arthrobacter spp. have appeared in the clinical microbiology literature. the aim of the present study was to elucidate the distribution of arthrobacter spp. and arthrobacter-like bacteria encountered in clinical specimens by studying 50 consecutively isolated or received strains of large-colony-forming, whiteish-grayish, non-cheese-like-smelling, nonfermentative gram- ... | 2008 | 18650355 |
| culture-independent nested pcr method reveals high diversity of actinobacteria associated with the marine sponges hymeniacidon perleve and sponge sp. | a culture-independent nested polymerase chain reaction (pcr) technique was used to investigate the diversity of actinobacteria communities associated with the sponges hymeniacidon perleve and sponge sp. the phylogenetic affiliation of sponge-derived actinobacteria was then assessed by 16s rrna sequencing of cloned dna fragments. a total of 196 positive clones were screened by restriction fragment length polymorphism (rflp) analysis; 48 unique operational taxonomic units (otus) were selected for ... | 2008 | 18670903 |
| chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading. | a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. complete cr(vi) reduction in aerobic cultures was achieved at a high concentration of 200 mg/l after incubation for only 65 hours. under anaerobic conditions up to 150 mg, cr(vi)/l was completely removed after incubating for 130 to 155 hours, still higher t ... | 2008 | 18701792 |
| coevolution between attine ants and actinomycete bacteria: a reevaluation. | we reassess the coevolution between actinomycete bacteria and fungus-gardening (attine) ants. actinomycete bacteria are of special interest because they are metabolic mutualists of diverse organisms (e.g., in nitrogen-fixation or antibiotic production) and because pseudonocardia actinomycetes are thought to serve disease-suppressing functions in attine gardens. phylogenetic information from culture-dependent and culture-independent microbial surveys reveals (1) close affinities between free-livi ... | 2008 | 18752608 |
| chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading: brits culture (south africa). | a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. cr(vi) reduction rate up to 8 times higher than the rate in previous cultures was achieved by the brits culture under aerobic conditions. near complete cr(vi) reduction was observed in batches under initial concentrations up to 200mg cr(vi)/l after incubati ... | 2008 | 18760438 |
| screening and distributing features of bacteria with hydantoinase and carbamoylase. | hydantoinase and carbamoylase are key biocatalysts for the production of optically pure amino acids from dl-5-substituted hydantoins (ssh). out of 364 isolated strains with hydantoinase and carbamoylase at 45 degrees c, 24 strains showed efficient hydantoinase and carbamoylase activities. among them, 17 produced l-amino acids, and 7 produced d-amino acids from both aromatic dl-5-benzylhydantoin and aliphatic dl-5-isopropylhydantoin. most of the strains that were able to form l-amino acid belonge ... | 2009 | 17498938 |
| conversion of puerarin into its 7-o-glycoside derivatives by microbacterium oxydans (cgmcc 1788) to improve its water solubility and pharmacokinetic properties. | microbacterium oxydans strain nj 6 isolated from soil samples converted puerarin into two novel compounds, puerarin-7-o-glucoside and puerarin-7-o-isomaltoside, via an unreported o-glycosylation of the phenolic hydroxyl group at the 7-position of puerarin. sucrose, maltotriose, and maltose could be used as glucosyl donors for glycosylation of puerarin, but uridine-diphosphate glucose, glucose, fructose, lactose, cyclodextrin, and starch could not. regardless of the position of b-ring in the (iso ... | 2008 | 18795283 |
| identities of microbacterium spp. encountered in human clinical specimens. | in the present study, 50 strains of yellow-pigmented gram-positive rods that had been isolated from human clinical specimens and collected over a 5-year period were further characterized by phenotypic and molecular genetic methods. all 50 strains belonged to the genus microbacterium, and together they represented 18 different species. microbacterium oxydans (n = 11), m. paraoxydans (n = 9), and m. foliorum (n = 7) represented more than half of the strains included in the present study. the isola ... | 2008 | 18799696 |
| a novel dps-type protein from insect gut bacteria catalyses hydrolysis and synthesis of n-acyl amino acids. | a novel type of a microbial n-acyl amino acid hydrolase (aah) from insect gut bacteria was purified, cloned and functionally characterized. the enzyme was obtained from microbacterium arborescens se14 isolated from the foregut of larvae of the generalist herbivore spodoptera exigua. the substrates of aah are n-acyl-glutamines previously reported to elicit plant defence reactions after introduction into the leaf during feeding. the isolated aah catalyses the hydrolysis of the amide bond (k(m) = 3 ... | 2007 | 17504494 |