| an outbreak of foodborne illness among attendees of a wedding reception in wisconsin likely caused by arcobacter butzleri. | arcobacter species, primarily arcobacter butzleri, are widely distributed among animals, infrequently isolated from humans, and previously not associated with outbreaks of foodborne illness. we report results of an investigation of a foodborne outbreak that occurred among attendees of a wedding reception in wisconsin, united states, and was likely caused by a. butzleri. | 2013 | 23379282 |
| assessment of the prevalence and diversity of emergent campylobacteria in human stool samples using a combination of traditional and molecular methods. | this study aims to assess the diversity of campylobacteria (campylobacter and arcobacter) in human fecal samples from patients with diarrhea (n = 140) and asymptomatic controls (n = 116) in chile, using a combination of traditional culture and molecular methods. the culture methods detected campylobacteria in 10.7% of the patients with diarrhea and in 1.7% of the controls. in contrast, the molecular methods detected campylobacteria more often than the traditional culture, with a prevalence of 25 ... | 2013 | 23375869 |
| genetic diversity, antibiotic resistance and biofilm-forming ability of arcobacter butzleri isolated from poultry and environment from a portuguese slaughterhouse. | the genus arcobacter is an emerging pathogen associated with several clinical symptoms. this genus is widely distributed and has been isolated from environmental, animal, food and human samples, where poultry is considered the major source. in this study, forty three arcobacter butzleri strains isolated from poultry and environment of a portuguese slaughterhouse, were characterized by pulsed field gel electrophoresis (pfge) and assessed for antimicrobial susceptibility and ability to form biofil ... | 2013 | 23369730 |
| distinct interactions of na+ and ca2+ ions with the selectivity filter of the bacterial sodium channel na(v)ab. | rapid and selective ion transport is essential for the generation and regulation of electrical signaling pathways in living organisms. in this study, we use molecular dynamics simulations and free energy calculations to investigate how the bacterial sodium channel na(v)ab (arcobacter butzleri) differentiates between na(+) and ca(2+) ions. multiple nanosecond molecular dynamics simulations revealed distinct binding patterns for these two cations in the selectivity filter and suggested a high affi ... | 2013 | 23261433 |
| antimicrobial susceptibility of an emergent zoonotic pathogen, arcobacter butzleri. | | 2012 | 23068601 |
| efficacy of wastewater treatment on arcobacter butzleri density and strain diversity. | arcobacter butzleri is a suspected waterborne enteric pathogen that is ubiquitous in the environment, but the degree to which wastewater treatment prevents entry of a. butzleri into environmental waters and the risks posed are not well established. untreated and treated wastewater samples (n = 260) were collected weekly from the lethbridge and fort macleod wastewater treatment facilities (the two major municipal inputs in southwestern alberta, canada) from may 2008 to april 2009. untreated waste ... | 2016 | 27636152 |
| occurrence of emerging food-borne pathogenic arcobacter spp. isolated from pre-cut (ready-to-eat) vegetables. | given that changes in consumer food behaviours have led to an increase in the demand for pre-cut ready-to-eat (rte) vegetables, and that few data are currently available on the occurrence of arcobacter spp. in such foods, the aim of the present study was to assess the occurrence of arcobacter spp. that carry virulence-associated genes on pre-cut rte vegetables, using cultural and molecular methods. arcobacter was detected using biomolecular identification methods in 44/160 (27.5%) of the samples ... | 2016 | 27442848 |
| short communication: arcobacter butzleri and arcobacter cryaerophilus survival and growth in artisanal and industrial ricotta cheese. | ricotta cheese is a ready-to-eat product with properties (ph >6.0, aw >0.98-0.99) and moisture content (75-80%) that may pose a risk to public health due to postprocess contamination by several bacterial pathogens, including arcobacters. the objective of the study was to evaluate the behavior of arcobacter butzleri and arcobacter cryaerophilus in ricotta cheese during its shelf life assuming postprocessing contamination. two types of ricotta cheese, artisanal water buffalo (wb) and industrial co ... | 2015 | 26233450 |
| genetic characterization of arcobacter isolates from various sources. | arcobacter is getting more attention due to its detection from wide host-range and foods of animal origin. the objective of this study was to determine the prevalence of arcobacter spp. in various sources at farm level and beef retailed in markets in malaysia and to assess the genetic relatedness among them. a total of 273 samples from dairy cattle including cattle (n=120), floor (n=30), water (n=18) and milk (n=105) as well as 148 beef samples collected from retail markets were studied. the ove ... | 2012 | 22739058 |
| emerging pathogen arcobacter spp. in acute gastroenteritis: molecular identification, antibiotic susceptibilities and genotyping of the isolated arcobacters. | the aims of this study were as follows: (i) to isolate arcobacter spp. from the stool samples of patients with gastroenteritis; (ii) to identify them with molecular methods; (iii) to genotype them using enterobacterial repetitive intergenic consensus (eric)-pcr; and (iv) to determine their antibiotic susceptibilities. for the study, a total of 3287 diarrhoeal stool samples submitted to the microbiology laboratory of the kayseri training and research hospital, kayseri, turkey, between 2010 and 20 ... | 2012 | 22700547 |
| arcobacter lanthieri sp. nov., isolated from pig and dairy cattle manure. | a study was undertaken to determine the prevalence and diversity of species of the genus arcobacter in pig and dairy cattle manure, which led to the identification of strains af1440t, af1430 and af1581. initially identified as arcobacter butzleri based on colony morphology and initial pcr-confirmation tests, analyses of 16s rrna gene sequences of these strains confirmed that they belonged to the genus arcobacter and were different from all known species of the genus. the isolates formed a distin ... | 2015 | 25977280 |
| prevalence and antimicrobial resistance of arcobacter butzleri and arcobacter cryaerophilus isolates from retail meat in lower silesia region, poland. | arcobacter butzleri and a. cryaerophilus are considered potential foodborne pathogens. consumption of arcobacter-contaminated food is regarded the most likely source of human poisoning. we investigated the prevalence and antimicrobial resistance of arcobacter isolates in 210 retail meat samples. seventy-nine a. butzleri and 6 a. cryaerophilus were isolated from pork, beef and chicken meat. incidence ofa. butzleri was found to be the highest in chicken meat (83%). less of a. butzleri was isolated ... | 2015 | 25928911 |
| metagenomic analysis of bacterial community composition and antibiotic resistance genes in a wastewater treatment plant and its receiving surface water. | the presence of pathogenic bacteria and the dissemination of antibiotic resistance genes (args) may pose big risks to the rivers that receive the effluent from municipal wastewater treatment plants (wwtps). in this study, we investigated the changes of bacterial community and args along treatment processes of one wwtp, and examined the effects of the effluent discharge on the bacterial community and args in the receiving river. pyrosequencing was applied to reveal bacterial community composition ... | 2016 | 27340885 |
| modified isolation method of arcobacter spp. from different environmental and food samples. | this study provides information on the occurrence of arcobacter in several types of water and food products of animal origin in the czech republic. we processed 190 samples using the modified method, and the occurrence of arcobacter spp. was confirmed in 36.8 % of these. this total incidence consisted of arcobacter butzleri (27.3 %), arcobacter cryaerophilus (8.4 %) and arcobacter skirrowii (1.1 %). we newly described the common presence of arcobacter spp. in sewage water in the czech republic t ... | 2015 | 25912846 |
| fate of antibiotic resistance genes and their associations with bacterial community in livestock breeding wastewater and its receiving river water. | large amounts of antibiotics are currently used in livestock breeding, which is the main driving factor contributing to the occurrence, spread and proliferation of antibiotic resistant bacteria (arb) and antibiotic resistance genes (args) in the environment. in this study, high-throughput sequencing based metagenomic approaches were employed to characterize the tempo-spacial changes of antibiotic resistome, bacterial community and their correlations in pig farming wastewater and its receiving ri ... | 2017 | 28763642 |
| virulence gene profiles of arcobacter species isolated from animals, foods of animal origin, and humans in andhra pradesh, india. | this study aimed to detect putative virulence genes in arcobacter species of animal and human origin. | 2017 | 28717327 |
| characterization of the emerging zoonotic pathogen arcobacter thereius by whole genome sequencing and comparative genomics. | four arcobacter species have been associated with human disease, and based on current knowledge, these gram negative bacteria are considered as potential food and waterborne zoonotic pathogens. at present, only the genome of the species arcobacter butzleri has been analysed, and still little is known about their physiology and genetics. the species arcobacter thereius has first been isolated from tissue of aborted piglets, duck and pig faeces, and recently from stool of human patients with enter ... | 2017 | 28671965 |
| application of long amplicon propidium monoazide-pcr to assess the effects of temperature and background microbiota on pathogens in river water. | the decay rates of enteric waterborne pathogens were evaluated following the introduction of yersinia enterocolitica, salmonella enterica, campylobacter jejuni and arcobacter butzleri into river water at different temperatures (5, 15 and 25°c) for a period of 28 days. to improve the accuracy of the results a molecular viability assay, long amplicon propidium monoazide-polymerase chain reaction (pma-pcr), was used to quantify the viable cell concentration and results from pcr with and without pma ... | 2017 | 28598346 |
| insights in the pathogenesis and resistance of arcobacter: a review. | arcobacter genus currently comprises 18 recognized species, among which arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii have been associated with human and animal disease. although these organisms, with special emphasis a. butzleri, are emerging as clinical pathogens, several aspects of their epidemiology and virulence are only starting to be clarified. in vitro human and animal cell culture assays have been used to show that several arcobacter species can adhere to and in ... | 2016 | 25806423 |
| detection of arcobacter spp. in mytilus galloprovincialis samples collected from apulia region. | the aim of the study was to evaluate the occurrence of arcobacter spp. in 20 samples of mytilus galloprovincialis purchased at fish markets in apulia region. the detection of arcobacter spp. was performed, after selective enrichment, on modified charcoal cefoperazone deoxycholate (mccd) agar supplemented with cefoperazone, amphotericin b and teicoplanin (cat). in 6 out of the 20 tested samples the presence of arcobacter spp. was found and confirmed by genus-based polymerase chain reaction. all t ... | 2015 | 27800382 |
| multilocus sequence typing reveals genetic diversity of foodborne arcobacter butzleri isolates in the north of spain. | the emerging pathogen arcobacter butzleri is being increasingly isolated from different animal food products but the routes of its transmission to human are not well established yet. typing methods would be useful in gaining such knowledge. here we report the great genetic diversity observed among a. butzleri isolates from different food products. forty-five isolates were analyzed by multilocus sequence typing (mlst). a total of 157 alleles were identified across all seven loci, ranging from 16 ... | 2014 | 25261830 |
| co-occurrence of free-living protozoa and foodborne pathogens on dishcloths: implications for food safety. | in the present study, the occurrence of free-living protozoa (flp) and foodborne bacterial pathogens on dishcloths was investigated. dishcloths form a potentially important source of cross-contamination with flp and foodborne pathogens in food-related environments. first various protocols for recovering and quantifying flp from dishcloths were assessed. the stomacher technique is recommended to recover flagellates and amoebae from dishcloths. ciliates, however, were more efficiently recovered us ... | 2014 | 25260173 |
| arcobacter butzleri induces a pro-inflammatory response in thp-1 derived macrophages and has limited ability for intracellular survival. | recent case reports have identified arcobacter (a.) butzleri to be another emerging pathogen of the family campylobacteraceae causing foodborne diseases. however, little is known about its interaction with the human immune system. as macrophages act as first defense against bacterial infections, we studied for the first time the impact of a. butzleri on human macrophages using thp-1 derived macrophages as an in vitro infection model. our investigations considered the inflammatory response, intra ... | 2014 | 25245281 |
| genotypic and phenotypic features of arcobacter butzleri pathogenicity. | even though arcobacter butzleri has been implicated in some human disease as diarrhoea and bacteraemia, much of its pathogenesis and virulence factors remain unclear. in this work we have compared pathogenic and genotypic properties of six a. butzleri isolates from human and non-human sources. the tested isolates showed to be susceptible to tetracyclines and aminoglycosides, however non-human isolates were all resistant to quinolones. the ability to form biofilms was variable among the tested st ... | 2014 | 25218724 |
| arcobacter butzleri in sheep ricotta cheese at retail and related sources of contamination in an industrial dairy plant. | this study aimed to evaluate arcobacter species contamination of industrial sheep ricotta cheese purchased at retail and to establish if the dairy plant environment may represent a source of contamination. a total of 32 sheep ricotta cheeses (1.5 kg/pack) packed in a modified atmosphere were purchased at retail, and 30 samples were collected in two sampling sessions performed in the cheese factory from surfaces in contact with food and from surfaces not in contact with food. seven out of 32 samp ... | 2014 | 25192995 |
| prevalence and antimicrobial susceptibility of arcobacter species in cow milk, water buffalo milk and fresh village cheese. | in this study, the presence of arcobacter spp. was examined in cow milk (n=50), water buffalo (wb) milk (n=50) and fresh village cheese (n=50) samples. the 16s rdna-rflp method was used for the identification of arcobacter spp. the disc diffusion method was used to investigate the susceptibility of all strains identified to 18 different antimicrobial substances. the most commonly isolated arcobacter species were found to be arcobacter butzleri (38.89%), arcobacter cryaerophilus (22.23%) and arco ... | 2014 | 25064812 |
| arcobacter: comparison of isolation methods, diversity, and potential pathogenic factors in commercially retailed chicken breast meat from costa rica. | arcobacter species have been recognized as potential food- and waterborne pathogens. the lack of standardized isolation methods and the relatively scarce knowledge about their prevalence and distribution as emerging pathogens are due to the limitations in their detection and identification. this study aimed to determine the presence and the identification of arcobacter in chicken breast samples commercially retailed in san josé, costa rica, as well as to describe the adherence and invasive poten ... | 2014 | 24853508 |
| resveratrol against arcobacter butzleri and arcobacter cryaerophilus: activity and effect on cellular functions. | the frequent isolation of arcobacter butzleri and arcobacter cryaerophilus from food samples makes it imperative to search for potential compounds able to inhibit the development of these bacteria. taking this into consideration, this study focuses on the antimicrobial activity of resveratrol and its mechanism of action against a. butzleri and a. cryaerophilus. the activity of resveratrol was assessed by a microdilution method and time-kill curves. resveratrol effect on cellular functions was as ... | 2014 | 24786554 |
| arcobacter spp. isolated from untreated domestic effluent. | arcobacter butzleri and arcobacter cryaerophilus were isolated from samples of raw untreated domestic sewage influent from nine separate wastewater treatment facilities in cheshire, uk. this is the first report of arcobacter spp. from sewage in the uk and suggests that arcobacter spp. may be present in the human community. | 2014 | 24666283 |
| arcobacter species and their pulsed-field gel electrophoresis genotypes in finnish raw milk during summer 2011. | the aim of this study was to investigate the occurrence of arcobacter species in raw milk in finland. a total of 177 raw milk samples, each from a separate farm, were examined from june to august 2011. arcobacter species were isolated using an enrichment and selective detection procedure. overall, 26 (15 % ) of the 177 samples yielded arcobacter spp. samples from 25 farms were positive for arcobacter butzleri and from 1 farm for arcobacter cryaerophilus. moreover, both arcobacter butzleri and a. ... | 2013 | 23992510 |
| occurrence and genetic diversity of arcobacter butzleri in an artisanal dairy plant in italy. | the present study aimed to investigate the presence, distribution, and persistence of arcobacter spp. in an artisanal dairy plant and to test the isolates to determine their different genotypes in the processing plant and in foods. samples were collected in an artisanal cheese factory on four occasions between october and december 2012. food samples (raw milk, ricotta cheese, mozzarella cheese, and conditioning liquid), water samples, and environmental samples were analyzed by the culture method ... | 2013 | 23974135 |
| prevalence and genetic diversity of arcobacter in food products in the north of spain. | the bacterial contamination of food products can cause serious public health problems. interest in arcobacter contamination has increased due to the relationship between these bacteria and human enteritis. we studied the prevalence and genetic diversity of arcobacter species at the retail level in the province of alava in basque country, spain. the results showed a high genetic diversity and indicated the regular presence of the main arcobacter spp. associated with human enteric illness in food ... | 2013 | 23905804 |
| bacterial pathogens isolated from raw meat and poultry compared with pathogens isolated from children in the same area of rural thailand. | to better understand the epidemiology of bacterial food borne pathogens in children, in relation to pathogens in meats from a market in rural thailand, we collected 73 cultures samples from raw chicken, pork and fish at a local market where diarrheal disease surveillance was conducted. standard methods were employed to isolate, identify and serotype enteric pathogens from children and food samples. antibiotic susceptibility testing was performed. ninety-seven percent of food samples were contami ... | 2013 | 23691636 |
| presence of virulence genes, adhesion and invasion of arcobacter butzleri. | the pathogenic potential of arcobacter butzleri isolates was investigated by detecting the presence of putative virulence genes and analysing the adhesive and invasive capabilities in cell cultures of human cell lines. | 2013 | 23647690 |
| isolation and identification of zoonotic species of genus arcobacter from chicken viscera obtained from retail distributors of the metropolitan area of san josé, costa rica. | arcobacter is a genus of growing importance worldwide; some of its species are considered emerging enteropathogens and potential zoonotic agents. in costa rica, as well as in other countries, its isolation has been reported, so the objective of this project was to evaluate and identify the presence of arcobacter in chicken viscera sold in the metropolitan area of san josé, costa rica, as well as to determine the antimicrobial resistance patterns associated with it. one hundred fifty samples of c ... | 2013 | 23643133 |
| quantitative proteomic analysis of the exoelectrogenic bacterium arcobacter butzleri ed-1 reveals increased abundance of a flagellin protein under anaerobic growth on an insoluble electrode. | exoelectrogens have the ability to generate electricity in mediator-less microbial fuel cells (mfcs) by extracellular electron transfer to the anode. we investigate the anode-specific responses of arcobacter butzleri ed-1, the first identified exoelectrogenic epsilonproteobacterium. itraq and 2d-lc ms/ms driven proteomics were used to compare protein abundances in a. butzleri ed-1 when generating an electronegative potential (-225 mv) in an anaerobic half-cell - either growing as an electrogenic ... | 2013 | 23063721 |
| [electricity generation from lactate using microbial fuel cell and the distribution characteristics of anode microbial community]. | two-chamber microbial fuel cells (mfcs) were set up to understand the electrogenic capacity of mfcs fed with lactic acid to investigate the distribution characteristics of microflora in the anode biofilm, supernatant, and sediment. | 2012 | 22934355 |
| prevalence and distribution of arcobacter spp. in raw milk and retail raw beef. | a total of 106 beef samples which consisted of local (n = 59) and imported (n = 47) beef and 180 milk samples from cows (n = 86) and goats (n = 94) were collected from selangor, malaysia. overall, 30.2% (32 of 106) of beef samples were found positive for arcobacter species. imported beef was significantly more contaminated (46.80%) than local beef (16.9%). arcobacter butzleri was the species isolated most frequently from imported (81.8%) and local (60%) beef, followed by arcobacter cryaerophilus ... | 2012 | 22856572 |
| crystal structure of a voltage-gated sodium channel in two potentially inactivated states. | in excitable cells, voltage-gated sodium (na(v)) channels activate to initiate action potentials and then undergo fast and slow inactivation processes that terminate their ionic conductance. inactivation is a hallmark of na(v) channel function and is critical for control of membrane excitability, but the structural basis for this process has remained elusive. here we report crystallographic snapshots of the wild-type na(v)ab channel from arcobacter butzleri captured in two potentially inactivate ... | 2012 | 22678296 |
| local anesthetic inhibition of a bacterial sodium channel. | recent structural breakthroughs with the voltage-gated sodium channel from arcobacter butzleri suggest that such bacterial channels may provide a structural platform to advance the understanding of eukaryotic sodium channel gating and pharmacology. we therefore set out to determine whether compounds known to interact with eukaryotic na(v)s could also inhibit the bacterial channel from bacillus halodurans and nachbac and whether they did so through similar mechanisms as in their eukaryotic homolo ... | 2012 | 22641643 |
| on conduction in a bacterial sodium channel. | voltage-gated na⁺-channels are transmembrane proteins that are responsible for the fast depolarizing phase of the action potential in nerve and muscular cells. selective permeability of na⁺ over ca²⁺ or k⁺ ions is essential for the biological function of na⁺-channels. after the emergence of the first high-resolution structure of a na⁺-channel, an anionic coordination site was proposed to confer na⁺ selectivity through partial dehydration of na⁺ via its direct interaction with conserved glutamate ... | 2012 | 22496637 |
| persistence of arcobacter butzleri ccug 30484 on plastic, stainless steel and glass surfaces. | the persistence of a. butzleri ccug 30484 on various surfaces under 32% and 64% relative humidity suspended in physiological saline or nutrient broth to simulate relatively clean or soiled conditions was studied using various isolation techniques. our study revealed that a. butzleri ccug 30484 cells were able to survive for a considerable period of time, even after the droplet of suspending medium has been visibly dried. an extended survival on polypropylene coupons at both humidity levels was o ... | 2008 | 24031258 |
| functionalized fullerene targeting human voltage-gated sodium channel, hnav1.7. | mutations of hnav1.7 that cause its activities to be enhanced contribute to severe neuropathic pain. only a small number of hnav1.7 specific inhibitors have been identified, most of which interact with the voltage-sensing domain of the voltage-activated sodium ion channel. in our previous computational study, we demonstrated that a [lys6]-c84 fullerene binds tightly (affinity of 46 nm) to navab, the voltage-gated sodium channel from the bacterium arcobacter butzleri. here, we extend this work an ... | 2017 | 28586206 |
| susceptibility to 18 drugs and multidrug resistance of arcobacter isolates from different sources within the czech republic. | arcobacter spp. are considered to be potential foodborne pathogens, and consumption of contaminated food containing these bacteria could endanger human and animal health. arcobacter butzleri and arcobacter cryaerophilus are the species most frequently isolated from food of animal origin and from other samples. the aim of this study was to evaluate the susceptibility of arcobacters isolated in the czech republic. no information about antibiotic susceptibility and multidrug resistance of arcobacte ... | 2017 | 28400212 |
| comparative isolation and genetic diversity of arcobacter sp. from fish and the coastal environment. | arcobacter species are emerging food-borne and water-borne human pathogens associated mostly with food animals and their environment. the present study was aimed to isolate arcobacter species from fish, shellfish and coastal water samples using two methods and to determine their genetic diversity. of 201 samples of fish, shellfish and water samples analysed, 66 (32·8%) samples showed the presence of arcobacter dna from both arcobacter enrichment broth and bolton broth. arcobacters were isolated ... | 2017 | 28394467 |