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helicobacter pylori directs tolerogenic programming of dendritic cells.our laboratory has shown that helicobacter pylori infection in mice triggers an increase in the number of subepithelial lamina propria cd11c(+) dendritic cells with luminal projections. the physical characteristic of these cells is consistent with their ability to traverse epithelial tight junctions as reported by maria recigno.3 gastric cd103(+) dendritic cells, which are known to induce mucosal regulatory t cells, were also increased in number, raising the question whether h. pylori infection ...201021327041
species identification and molecular characterization of acinetobacter spp. blood culture isolates from norway.the study investigated the species distribution, antibiotic susceptibility patterns and genotypic resistance characteristics of 113 consecutive blood culture isolates of acinetobacter species collected between 2005 and 2007 throughout norway.201121393175
improved resistance against botrytis cinerea by grapevine-associated bacteria that induce a prime oxidative burst and phytoalexin production.bacteria such as pantoea agglomerans (pa-af2), bacillus subtilis (bs-271), acinetobacter lwoffii (al-113), and pseudomonas fluorescens (pf-ct2), originating from the vineyard, can induce defense responses and enhance resistance of grapevine against the fungal pathogen botrytis cinerea. the perception of these bacteria by plant cells or tissues in relation to their activities remains unknown. in this study, we examined the relationships between the activity of each bacterium to induce or prime so ...201121425931
contrasting effects of ultraviolet radiation on the growth efficiency of freshwater bacteria.in this study, we tested the hypothesis that the growth efficiency of freshwater bacteria is differentially affected by ultraviolet radiation (uvr, 280-400 nm) as mediated through changes in their production and respiration rates. five bacterial strains affiliated to alphaproteobacteria, betaproteobacteria, gammaproteobacteria, and actinobacteria were isolated from different freshwater habitats and exposed in the laboratory to photosynthetically active radiation (par) and par + uvr, or kept in t ...201121516253
bacteremia caused by laribacter hongkongensis misidentified as acinetobacter lwoffii: report of the first case in korea.laribacter hongkongensis is an emerging pathogen in patients with community-acquired gastroenteritis and traveler's diarrhea. we herein report a case of l. hongkongensis infection in a 24-yr-old male with liver cirrhosis complicated by wilson's disease. he was admitted to a hospital with only abdominal distension. on day 6 following admission, he complained of abdominal pain and his body temperature reached 38.6℃. the results of peritoneal fluid evaluation revealed a leukocyte count of 1,180/µl ...201121532861
evaluation of the impact of dna extraction methods on bac bacterial community composition measured by denaturing gradient gel electrophoresis.aims:  the impact of dna extraction methods on biological activated carbon (bac) dna yield and bacterial community was evaluated. methods and results:  three different dna extraction methods were compared: method a, method b and method c. method c with ultrasonic pretreatment improved cell lysis efficiency (from 34% to 87%) and dna yield [from 10·58 μg g(-1) (dry wt) of carbon to 21·42 μg g(-1) (dry wt) of carbon]. denaturing gradient gel electrophoresis profiles obtained by method c recovered t ...201121535041
biofilm formation by acinetobacter baumannii strains isolated from urinary tract infection and urinary catheters.fifty acinetobacter isolates were obtained from urinary tract infections (uti) and urinary catheter samples. analytical profile index (api) assays identified forty-seven isolates as a. baumannii and three as a. lwoffii. six a. baumannii isolates (a1-a6) displayed hydrophobicity indices >70 %. twenty isolates exhibited lectin activity. biofilm formation by these isolates was compared to those with low hi values (a45-a50). biofilms on different surfaces were confirmed by light microscopy, epifluor ...201121569125
clinical impact and molecular basis of antimicrobial resistance in non-baumannii acinetobacter.species of acinetobacter other than acinetobacter baumannii are involved in nosocomial infections. acinetobacter lwoffii, acinetobacter genomospecies 3 and acinetobacter genomospecies 13tu are found in community- and nosocomial-acquired infections as well as in neonatal intensive care units. the non-baumannii acinetobacter are normally highly susceptible to ciprofloxacin, ampicillin/sulbactam, gentamicin and tigecycline. carbepenems show good activity although resistant isolates have been report ...201121585259
[a case of acute phlegmonous gastritis causing gastroparesis and cured with medical treatment alone.]acute phlegmonous gastritis is an uncommon disease, often fatal condition characterized by suppurative bacterial infection of the gastric wall. it has a high mortality rate mainly because the diagnosis is usually made late. until recently, gastrectomy in combination with antibiotics was recommended. we had experienced a case of 66-year-old man presented with epigastric pain, nausea, vomiting, and hematemesis, followed by aspiration pneumonia. at upper gastrointestinal endoscopy, the gastric lume ...201121623140
epigenetic regulation in murine offspring as a novel mechanism for transmaternal asthma protection induced by microbes.bronchial asthma is a chronic inflammatory disease resulting from complex gene-environment interactions. natural microbial exposure has been identified as an important environmental condition that provides asthma protection in a prenatal window of opportunity. epigenetic regulation is an important mechanism by which environmental factors might interact with genes involved in allergy and asthma development.201121680015
whole-genome sequence of a multidrug resistant clinical isolate of acinetobacter lwoffii.acinetobacter lwoffii has been considered as opportunistic pathogen that can cause nosocomial infections in humans. here, we present the genome sequence of a clinical isolate, a. lwoffii wj10621, a multidrug resistant isolate that carries a plasmid with ndm-1 resistance gene.201121742884
a novel influenza a h1n1 clinical manifestations in patients at chiang mai university hospital.to describe the clinical manifestations of patients affected with a novel influenza a (h1n1 2009) during the pandemic.201121863671
reliability of kirby-bauer disk diffusion method for detecting meropenem resistance among non-fermenting gram-negative bacilli.meropenem is empirically used as a last resort for the treatment of infections by non-fermenting gram-negative bacilli (nfgnb). minimum inhibitory concentration (mic) determined using agar or broth dilution methods is widely used for testing meropenem resistance. however, it is not possible in resource-poor settings.201121934220
antimicrobial activity and cytotoxic effects of magnolia dealbata and its active compounds.multi-drug resistance is of great concern for public health worldwide and necessitates the search for new antimicrobials from sources such as plants. several magnolia (magnoliaceae) species have been reported to exert antimicrobial effects on sensitive and multidrug-resistant microorganisms. however, the antimicrobial properties of magnolia dealbata have not been experimentally evaluated. the antimicrobial effects of an ethanol extract of magnolia dealbata seeds (mde) and its active compounds ho ...201121922914
nonfermenting gram-negative bacilli infections in a tertiary care hospital in kolar, karnataka.aim: nonfermenting gram-negative bacilli (nfgnb), which are saprophytic in nature, have emerged as important healthcare-associated pathogens. they exhibit resistance not only to beta lactam and the other groups of antibiotics, but also to carbapenems. this study was undertaken to identify the nonfermenters isolated from various clinical samples, to assess their clinical significance, to know the type of healthcare-associated infections they caused, and to know their anti-microbial sensitivity pa ...200921938252
Acinetobacter community-acquired pneumonia in a healthy child.Acinetobacter is involved in a variety of infectious diseases primarily associated with healthcare. Recently there has been increasing evidence of the important role these pathogens play in community acquired infections. We report on the case of a previously healthy child, aged 28 months, admitted for fever, cough and pain on the left side of the chest, which on radiographic examination corresponded to a lower lobe necrotizing pneumonia. After detailed diagnostic work-up, community acquired Acin ...201121963110
identification of the naturally occurring genes encoding carbapenem-hydrolysing oxacillinases from acinetobacter haemolyticus, acinetobacter johnsonii, and acinetobacter calcoaceticus.clin microbiol infect abstract: carbapenem resistance is increasingly being reported among acinetobacter species, and results mostly from the expression of acquired carbapenem-hydrolysing oxacillinases (chdls). several acinetobacter species intrinsically possess chromosomal chdl genes: acinetobacter baumannii (bla(oxa-51) ), acinetobacter radioresistens (bla(oxa-23) ), and acinetobacter lwoffii (bla(oxa-134) ). we aimed to identify the progenitors of novel chdl-encoding genes for identification ...201122128805
genes encoding oxa-134 like enzymes are found in acinetobacter lwoffii/ genomic species 9 and a. schindleri and can be used for identification.genes encoding bla(oxa-134) and variants were sought in 21 species of acinetobacter and found in a. lwoffii, genomic species 9 (regarded as synonyms) and a. schindleri. sequencing revealed a 9 bp deletion in the gene in the type strain of genomic species 9 (atcc 9957) relative to that in the type strain of a. lwoffii (atcc 15309). primers based on the non-deleted gene gave specific amplification of 29/30 clinical isolates of a. lwoffii/genomic species 9.201122205817
suspected seminal vesiculitis in an asian elephant (elephas maximus).a 32-year-old male asian elephant (elephas maximus) underwent routine transrectal stimulation for semen collection as part of an artificial insemination program. the procedure consisted of a preinsemination semen collection followed by two consecutive days of semen collections for artificial insemination. the second day's sample contained large numbers of inflammatory cells, intracellular bacteria, and phagocytized sperm. semen was submitted for culture and sensitivity. culture revealed acinetob ...201122950323
the use of multiplex real-time pcr improves the detection of the bacterial etiology of community acquired pneumonia.community-acquired pneumonia (cap) is still a major cause of morbidity and mortality especially to children and compromised hosts, such as the old and those with underlying chronic diseases. knowledge of pathogens causing cap constitutes the basis for selection of antimicrobial treatment. previous data have shown that etiological agents can be identified in only up to 50% of patients, but this figure can be improved by using polymerase chain reaction (pcr). this study was designed to evaluate mu ...201122433882
antimicrobial and cytotoxic effects of mexican medicinal plants.the antimicrobial effects of the mexican medicinal plants guazuma ulmifolia, justicia spicigera, opuntia joconostle, o. leucotricha, parkinsonia aculeata, phoradendron longifolium, p. serotinum, psittacanthus calyculatus, tecoma stans and teucrium cubense were tested against several human multi-drug resistant pathogens, including three gram (+) and five gram (-) bacterial species and three fungal species using the disk-diffusion assay. the cytotoxicity of plant extracts on human cancer cell line ...201122312741
acinetobacter lwoffii peritonitis in a patient receiving continuous ambulatory peritoneal dialysis. 201223379502
bla(imp) and bla(vim) mediated carbapenem resistance in pseudomonas and acinetobacter species in india.the emergence and rapid spread of blaimp and blavim metallo-beta-lactamase (mbl) producing gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options.201223277500
functional metagenomics of spacecraft assembly cleanrooms: presence of virulence factors associated with human pathogens.strict planetary protection practices are implemented during spacecraft assembly to prevent inadvertent transfer of earth microorganisms to other planetary bodies. therefore, spacecraft are assembled in cleanrooms, which undergo strict cleaning and decontamination procedures to reduce total microbial bioburden. we wanted to evaluate if these practices selectively favor survival and growth of hardy microorganisms, such as pathogens. three geographically distinct cleanrooms were sampled during the ...201627667984
diversity and antibiograms of bacterial organisms isolated from samples of household drinking-water consumed by hiv-positive individuals in rural settings, south africa.diarrhoea is a hallmark of hiv infections in developing countries, and many diarrhoea-causing agents are often transmitted through water. the objective of the study was to determine the diversity and antibiotic susceptibility profiles of bacterial organisms isolated from samples of household drinking-water consumed by hiv-infected and aids patients. in the present study, household water stored for use by hiv-positive patients was tested for microbial quality, and isolated bacterial organisms wer ...201223082625
epidemiology of bacteremia caused by uncommon non-fermentative gram-negative bacteria.prevalence of bacteremia caused by non-fermentative gram-negative bacteria (nfgnb) has been increasing over the past decade. although many studies have already investigated epidemiology of nfgnb bacteremia, most focused only on common nfgnb including pseudomonas aeruginosa (pa) and acinetobacter baumannii (ab). knowledge of uncommon nfgnb bacteremia is very limited. our study aimed to investigate epidemiology and identify factors associated with uncommon nfgnb bacteremia.201323566113
identification of host-immune response protein candidates in the sera of human oral squamous cell carcinoma patients.one of the most common cancers worldwide is oral squamous cell carcinoma (oscc), which is associated with a significant death rate and has been linked to several risk factors. notably, failure to detect these neoplasms at an early stage represents a fundamental barrier to improving the survival and quality of life of oscc patients. in the present study, serum samples from oscc patients (n = 25) and healthy controls (n = 25) were subjected to two-dimensional gel electrophoresis (2-de) and silver ...201425272005
identification of new delhi metallo-β-lactamase 1 in acinetobacter lwoffii of food animal origin.to investigate the presence of metallo-β-lactamase (mbl) genes and the genetic environment of the new delhi metallo-β-lactamase gene bla(ndm-1) in bacteria of food animal origin.201222629360
gene-silencing antisense oligomers inhibit acinetobacter growth in vitro and in vivo.peptide-conjugated phosphorodiamidate morpholino oligomers (ppmos) are synthetic dna/rna analogues that silence expression of specific genes. we studied whether ppmos targeted to essential genes in acinetobacter lwoffii and acinetobacter baumannii are active in vitro and in vivo.201324130069
first report of wohlfahrtiimonas chitiniclastica isolation from a patient with cellulitis in the united states.we report the first documented isolation of wohlfahrtiimonas chitiniclastica from a human in the united states. initially misidentified as acinetobacter lwoffii by vitek-2, the isolate was subsequently identified as w. chitiniclastica by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) and 16s rrna gene sequencing. while the clinical significance of the isolate in this case is unclear, it highlights the superior performance of maldi-tof ms for bacterial ...201526378273
evaluation of a parchment document, the 13th century incorporation charter for the city of krakow, poland, for microbial hazards.the literature of environmental microbiology broadly discusses issues associated with microbial hazards in archives, but these publications are mainly devoted to paper documents. there are few articles on historical parchment documents, which used to be very important for the development of literature and the art of writing. these studies present a broad spectrum of methods for the assessment of biodeterioration hazards of the parchment document in question. they are based on both conventional m ...201626896133
novel plasmid and its variant harboring both a bla(ndm-1) gene and type iv secretion system in clinical isolates of acinetobacter lwoffii.the spread of the bla(ndm-1) gene is gaining worldwide attentions. this gene is usually carried by large plasmids and has been discovered in diverse bacteria since it was originally found in klebsiella pneumoniae. here we report the complete sequences of a bla(ndm-1)-bearing plasmid, pndm-bj01, and its variant, pndm-bj02, isolated from clinical acinetobacter lwoffii strains. the plasmid pndm-bj01 is 47.3 kb in size and cannot be classified into any known plasmid incompatibility group, thus repre ...201222290961
a case report of a multi-drug resistant bacterial infection in a diabetic patient treated in northeast brazil.diabetes mellitus is one of the most critical health conditions around the world, not only in terms of the number of affected people, disability, and premature mortality, but also in regards to the health care costs involved in controlling and treating its complications. among the most constant ailments the diabetic patient suffers is the diabetic foot, defined as any infection, ulceration, and/or necrosis of deep tissues associated with neurological abnormalities and various degrees of peripher ...201222745851
comparison between phenotypic and pcr for detection of oxa-23 type and metallo-beta-lactamases producer acinetobacter spp.resistance to carbapenems is developing around the world and can cause many problems for treatment of patients. production of metallo-beta-lactamase (mbl) is one of the main mechanism for this type of resistance. so, detection of mbl-producer microorganisms can prevent the spread of this type of resistance.201324327942
complete genome analysis of three acinetobacter baumannii clinical isolates in china for insight into the diversification of drug resistance elements.the emergence and rapid spreading of multidrug-resistant acinetobacter baumannii strains has become a major health threat worldwide. to better understand the genetic recombination related with the acquisition of drug-resistant elements during bacterial infection, we performed complete genome analysis on three newly isolated multidrug-resistant a. baumannii strains from beijing using next-generation sequencing technology.201323826102
detection of new delhi metallo-β-lactamase (encoded by blandm-1) in acinetobacter schindleri during routine surveillance.a carbapenem-resistant alcaligenes faecalis strain was isolated from a surveillance swab of a service member injured in afghanistan. the isolate was positive for bla(ndm) by real-time pcr. species identification was reevaluated on three identification systems but was inconclusive. genome sequencing indicated that the closest relative was acinetobacter schindleri and that bla(ndm-1) was carried on a plasmid that shared >99% identity with one identified in an acinetobacter lwoffii isolate. the iso ...201323554204
efficacy and safety of lomefloxacin on bacterial extraocular disease in the horse.lomefloxacin is a broad-spectrum fluoroquinolone antibiotic used for the treatment of bacterial extraocular disease. this study investigated the efficacy and safety of lomefloxacin eye drops for bacterial extraocular disease in horses. lomefloxacin ophthalmic solution (0.3%) was instilled three times daily for 2-5 days in 65 horses diagnosed with bacterial extraocular disease based on clinical findings. clinical observations and bacteriological examinations were performed at the start of treatme ...201525787926
pyogenic liver abscess caused by acinetobacter lwoffii: a case report.acinetobacter lwoffii is a gram negative aerobic non-fermenter bacilli. it is considered as an important emerging pathogen after acinetobacter baumannii in patients with impaired immune system and in nosocomial infections. here, we present a case of community acquired pyogenic liver abscess caused by acinetobacter lwoffii in a diabetic patient.201627504286
development and evaluation of species-specific pcr for detection of nine acinetobacter species.molecular methods have the potential to improve the speed and accuracy of acinetobacter species identification in clinical settings. the goal of this study is to develop species-specific pcr assays based on differences in the rna polymerase beta-subunit gene (rpob) to detect nine commonly isolated acinetobacter species including acinetobacter baumannii, acinetobacter calcoaceticus, acinetobacter pittii, acinetobacter nosocomialis, acinetobacter lwoffii, acinetobacter ursingii, acinetobacter bere ...201627312551
the ancient small mobilizable plasmid palwed1.8 harboring a new variant of the non-cassette streptomycin/spectinomycin resistance gene aada27.the small mobilizable plasmid palwed1.8 containing a novel variant of the streptomycin/spectinomycin resistance gene aada27 was isolated from the permafrost strains of acinetobacter lwoffii. the 4135bp plasmid carries mobа and mobc genes that mediate its mobilization by conjugative plasmids. the nucleotide sequences of mobа and mobc are similar to those of mobilization genes of the modern plasmid pray* and its variants, which contain aadb gene, and are widespread among the pathogenic strains of ...201726896789
a case of disseminated intravascular coagulation secondary to acinetobacter lwoffii and acinetobacter baumannii bacteremia.bacteremia is currently one of the infections with the highest mortality in hospitals [1]. acinetobacter lwoffii and acinetobacter baumannii are gram-negative bacteria and both represent opportunistic pathogens. in certain cases, the management can be challenging since these organisms can be highly resistant to antimicrobial agents. clinical illnesses associated with acinetobacter include pneumonia, meningitis, peritonitis, endocarditis and infections of the urinary tract and skin [1]. acinetoba ...201526793461
acinetobacter lwoffii an emerging pathogen in neonatal icu.acinetobacter species are ubiquitous in the environment and are important causative agent for nososcomial infection especially in immunocompromised patients. multi drug resistant acinetobacter lwoffii are emerging as a pathogen in neoanatal sepsis.201526307173
co-production of esbl and ampc β-lactamases in clinical isolates of a. baumannii and a. lwoffii in a tertiary care hospital from northern india.acinetobacter baumannii is an important cause of health care associated infections which are difficult to control and treat, because of widespread antimicrobial resistance which is possessed by this organism.201424959443
identification of 50 class d β-lactamases and 65 acinetobacter-derived cephalosporinases in acinetobacter spp.whole-genome sequencing of a collection of 103 acinetobacter strains belonging to 22 validly named species and another 16 putative species allowed detection of genes for 50 new class d β-lactamases and 65 new acinetobacter-derived cephalosporinases (adc). all oxacillinases (oxa) contained the three typical motifs of class d β-lactamases, stfk, (f/y)gn, and k(s/t)g. the phylogenetic tree drawn from the oxa sequences led to an increase in the number of oxa groups from 7 to 18. the topologies of th ...201424277043
multiplex pcr to detect the genes encoding naturally occurring oxacillinases in acinetobacter spp.bacteria of the genus acinetobacter are increasingly being isolated in hospitals and are recognized as emerging nosocomial pathogens. species identification is difficult and there is a need for simple molecular methods to differentiate between the species. naturally occurring oxacillinase genes (blaoxa) have been identified in several acinetobacter species and their detection by pcr can aid in species identification. the aim of this study was to develop a multiplex pcr to identify intrinsic blao ...201424288029
antioxidant, antimicrobial and antiverotoxic potentials of extracts of curtisia dentata.there is an increase in antimicrobial resistance and complexities arising from verotoxic related bacterial infections as well as rise in demand for application of natural antioxidants to combat oxidative damage by free radicals in many oxidative stress-mediated disease conditions such as cancer. thus the potential of curtisia dentata as antimicrobial, antioxidant and antiverotoxin against environmental isolates of escherichia coli and acinetobacter spp. as well as the presence of phytochemicals ...201222504170
blandm-1-carrying acinetobacter johnsonii detected in hospital sewage.this study screened hospital sewage for the presence of blandm.201323288403
identification of lama glama as reservoirs for acinetobacter lwoffii.south american camelids have an increasing relevance in local economies, worldwide. these animals are bred for their meat, fur and as companion and therapy animals. thus, their sanitary status should be well-established. according to the oie (world organization for animal health), respiratory infections mainly produced by pasteurella spp. have been reported for camelids. it has been stated that this microorganism causes a mild disease, although many authors report it is an important cause of mor ...201728303121
effects of biochars and mwnts on biodegradation behavior of atrazine by acinetobacter lwoffii dns32.whether the biodegradation of atrazine by acinetobacter lwoffii dns32 can have a difference in the presence of two representative carbon materials (cms), namely, biochars (bcs) and carbon nanotubes (cnts) is explored, through investigating the influence of cms on the biodegradation rate, the viability of bacteria and the expression of atrazine genes in aqueous medium. multi-walled carbon nanotubes (mwnts), biochars resulted from corn straws (c-bcs) and that made from banana peels (b-bcs) were ch ...201727802884
resistance of permafrost and modern acinetobacter lwoffii strains to heavy metals and arsenic revealed by genome analysis.we performed whole-genome sequencing of five permafrost strains of acinetobacter lwoffii (frozen for 15-3000 thousand years) and analyzed their resistance genes found in plasmids and chromosomes. four strains contained multiple plasmids (8-12), which varied significantly in size (from 4,135 to 287,630 bp) and genetic structure; the fifth strain contained only two plasmids. all large plasmids and some medium-size and small plasmids contained genes encoding resistance to various heavy metals, incl ...201627795957
detection of multi-drug resistant acinetobacter lwoffii isolated from soil of mink farm.there were 4 acinetobacter lwoffii obtained from soil samples. the antimicrobial susceptibility of the strains to 16 antimicrobial agents was investigated using k-b method. three isolates showed the multi-drug resistance. the presence of resistance genes and integrons was determined using pcr. the aada1, aac(3')-iic, aph(3')-vii, aac(6')-ib, sul2, cat2, flor, and tet(k) genes were detected, respectively. three class 1 integrons were obtained. the arr-3-aaca4 and blapse-1 gene cassette, which cau ...201627554122
degradation capacities of bacteria and yeasts isolated from the gut of dendroctonus rhizophagus (curculionidae: scolytinae).bark beetles (curculionidae: scolytinae) feed on the xylem and phloem of their host, which are composed of structural carbohydrates and organic compounds that are not easily degraded by the insects. some of these compounds might be hydrolyzed by digestive enzymes produced by microbes present in the gut of these insects. in this study, we evaluated the enzymatic capacity of bacteria (acinetobacter lwoffii, arthrobacter sp., pseudomonas putida, pseudomonas azotoformans, and rahnella sp.) and yeast ...201727544667
draft genome sequence and complete plasmid sequence of acinetobacter lwoffii f78, an isolate with strong allergy-protective properties.the hygiene hypothesis states that the tremendous increase in atopic diseases correlates significantly with less contact to microbes in childhood. here, we report the draft genome sequence of acinetobacter lwoffii f78, a rural cowshed isolate with strong allergy-protective properties that contains an 8,579-bp plasmid.201627445377
isolation and characterization of α-endosulfan degrading bacteria from the microflora of cockroaches.extensive applications of organochlorine pesticides like endosulfan have led to the contamination of soil and environments. five different bacteria were isolated from cockroaches living in pesticide contaminated environments. according to morphological, physiological, biochemical properties, and total cellular fatty acid profile by fatty acid methyl esters (fames), the isolates were identified as pseudomonas aeruginosa g1, stenotrophomonas maltophilia g2, bacillus atrophaeus g3, citrobacter amol ...201627281995
proteogenomic characterization of monocyclic aromatic hydrocarbon degradation pathways in the aniline-degrading bacterium burkholderia sp. k24.burkholderia sp. k24, formerly known as acinetobacter lwoffii k24, is a soil bacterium capable of utilizing aniline as its sole carbon and nitrogen source. genomic sequence analysis revealed that this bacterium possesses putative gene clusters for biodegradation of various monocyclic aromatic hydrocarbons (mahs), including benzene, toluene, and xylene (btx), as well as aniline. we verified the proposed mah biodegradation pathways by dioxygenase activity assays, rt-pcr, and lc/ms-based quantitati ...201627124467
bacterial and fungal contamination risks in human oocyte and embryo cryopreservation: open versus closed vitrification systems.to study the contamination risk in open and closed vitrification devices for oocyte/embryo cryopreservation by evaluating the contaminants present (bacteria and fungi) in the thaw medium and in liquid nitrogen (ln) storage containers.201627025548
new delhi metallo-β-lactamase-1-producing acinetobacter lwoffii of companion animal origin in china. 201526470987
domestic wastewater treatment in a novel sequencing batch biofilm filter.biological treatment of domestic sewage low c/n ratio was accomplished in a pilot-scale sequencing batch biofilm filter (sbbf). the novel hybrid bioreactor consisted of bio-band in the upper and anthracite filter media in the bottom, which combined a sequencing batch biofilm reactor (sbbr) with a biological filter. the average removal efficiency values of chemical oxygen demand (cod), ammonium nitrogen (nh4 (+)-n), total nitrogen (tn), and total phosphorus (tp) were 89.4, 83.3, 62.9, and 48.7%, ...201525967659
postcataract surgery endophthalmitis caused by acinetobacter lwoffii.acinetobacter lwoffii is a rare cause of endophthalmitis. we report a case of acute postoperative endophthalmitis in a female, who was treated successfully with pars plana vitrectomy and intravitreal antibiotics.201725949088
microbial contamination of glaucoma eyedrops used by patients compared with ocular medications used in the hospital.the aim of this study was to compare the percentage of contamination of multiuse eyedrops applied by glaucoma patients at home and by the medical personnel at the outpatient department, the ward, and the operating room of our department of ophthalmology. eyedrops were collected over a period of 11 months. samples were taken from the dropper tip (smear), drops, and the residual fluid inside the bottle and cultivated on blood agar. colony forming units were counted and identified by mass spectrome ...201525715262
inflammatory responses and potencies of various lipopolysaccharides from bacteria and cyanobacteria in aquatic environments and water supply systems.inflammatory substances derived from indigenous bacteria in aquatic environments or water systems are of great concern. lipopolysaccharides (lpss), one of the major inflammatory substances in water, are usually identified using limurus amoebocyte lysate (lal) assay on the basis of their endotoxic activity, but endotoxin levels do not accurately represent their inflammatory potency in humans. in this investigation, the cellular endotoxin contents of pure-cultured bacteria/cyanobacteria, which are ...201525666398
a novel new delhi metallo-β-lactamase variant, ndm-14, isolated in a chinese hospital possesses increased enzymatic activity against carbapenems.a novel new delhi metallo-β-lactamase (ndm) variant, ndm-14, was identified in clinical isolate acinetobacter lwoffii jn49-1, which was recovered from an intensive care unit patient at a local hospital in china. ndm-14, which differs from other existing enzymes by an amino acid substitution at position 130 (asp130gly), possesses enzymatic activity toward carbapenems that is greater than that of ndm-1. kinetic data indicate that ndm-14 has a higher affinity for imipenem and meropenem.201525645836
antibacterial, antioxidant, antifungal and anti-inflammatory activities of crude extract from nitraria schoberi fruits.this study is the first comprehensive investigation of the antibacterial, antioxidant, antifungal and anti-inflammatory activities of a crude extract from nitraria schoberi l. (zygophyllaceae) fruits. the extract was tested against four gram-negative (pseudomonas aeruginosa, enterobacter aerogenes, klebsiella pneumoniae and acinetobacter lwoffii) and one gram-positive (staphylococcus aureus) bacteria using the agar disc diffusion and microdilution methods. p. aeruginosa was inhibited the most (w ...201528324518
[intestine microbiota and allergic diseases].in industrialized countries an increased number of diseases due to immune system disorders including connected with allergy is noted. allergic diseases generally proceed against the background of various common inflammatory diseases arising in childhood. the role of intestine microflora in its interaction with immune system and defining factors in allergization of children are actively studied. a decrease of risk of allergy development later in life for children who had grown up in the countrysi ...201425286512
acinetobacter species in the skin microbiota protect against allergic sensitization and inflammation.the human commensal microbiota interacts in a complex manner with the immune system, and the outcome of these interactions might depend on the immune status of the subject.201425262465
mycotic abdominal aortic aneurysm caused by bacteroides thetaiotaomicron and acinetobacter lwoffii: the first case in korea.mycotic aneurysms are uncommon, but are fatal without appropriate management. previous reports have shown that anaerobes and gram-negative organisms are less common but more dangerous than other causative agents of mycotic aneurysm. we report the case of a 60-year-old man with poorly controlled diabetes mellitus and atherosclerosis in the aorta, and a 10-day of history of lower abdominal pain and fever. this man was diagnosed with an uncommon abdominal aorta mycotic aneurysm caused by bacteroide ...201424693472
acinetobacter harbinensis sp. nov., isolated from river water.a bacterial strain, hitli 7t, with nitrifying ability was isolated from the surface water of the songhua river in china. cells were gram-stain-negative, strictly aerobic, oxidase-negative, non-motile coccobacilli, capable of growth in mineral media with acetate as the sole carbon source and ammonia as the sole source of nitrogen. the cells did not grow at 37 °c, but did grow at 2 °c. the dna g+c content was 45.5 mol%. results of 16s rrna gene sequence analysis indicated a close relationship betw ...201424478215
complete sequences of a novel blandm-1-harbouring plasmid from providencia rettgeri and an fii-type plasmid from klebsiella pneumoniae identified in canada.emergence of plasmids harbouring bla(ndm-1) is a major public health concern due to their association with multidrug resistance and their potential mobility.201424275114
bacterial pleuritis with thickened mesothelial hyperplasia in a young beagle dog.a five-month-old male beagle dog suddenly became moribund. bloody fluid accumulated in the thoracic and abdominal cavities, and soft yellow flecks were floating in the thoracic fluid. the mediastinum and pericardium became dark reddish with villous thickening. other parietal and pulmonary pleurae were rough, and the organs adhered to each other. histologically, most mediastinal pleura formed papillary projections covered by a single layer of mesothelial cells. many macrophages and neutrophils in ...201324155565
bacteremia in patients with acute pancreatitis as revealed by 16s ribosomal rna gene-based techniques*.to define the characteristic of bacteremia in patients with acute pancreatitis and determine its possible association with the disease severity.201323863226
prevalence and bacteriology of bacteremia associated with cleft lip and palate surgery.the aim of the study was to determine the prevalence and bacteriology of bacteremia associated with cleft lip and palate (clp) surgery. three venous blood samples were obtained from 90 eligible subjects who presented for clp surgery: before surgical incision, 1 minute after placement of the last suture, and 15 minutes thereafter. the samples were injected into an oxoid signal blood culture and transported to the laboratory for gram-positive/negative and aerobic/anaerobic bacteria analysis. preva ...201323851754
bacteremia in the patients with acute pancreatitis as revealed by 16s ribosomal rna gene-based techniques.objectives:: to define the characteristic of bacteremia in the patients with acute pancreatitis and determine its possible association with the disease severity. design:: a prospective controlled study. setting:: icu of jinling hospital, china. patients:: a total of 48 patients with mild or severe acute pancreatitis were enrolled in this study. interventions:: none. measurements and main results:: samples of peripheral blood were collected from the patients at 4 or 5 and 9 or 10 days after acute ...201323764972
[comparison of the adaptation potential of arthrobacter oxydans and acinetobacter lwoffii isolated from permafrost sediments with their collection analogues]. 201323718046
[fitness costs of blandm-1 bearing plasmid pndm-bj01 in acinetobacter lwoffii].to investigate the fitness costs of the new delhi metallo-beta-lactamase-1 (biandm-1) bearing plasmid pndm-bj01 in acinetobacter lwoffii strain 10621, and to evaluate the potentials of the sustainahility and expansions among the acinetobacter lwoffii population.201323614246
cystic fibrosis transmembrane conductance regulator knockout mice exhibit aberrant gastrointestinal microbiota.the composition of the gastrointestinal microbiome is increasingly recognized as a crucial contributor to immune and metabolic homeostasis-deficiencies in which are characteristic of cystic fibrosis (cf) patients. the murine model (cftr (-/-) , cf), has, in previous studies, demonstrated characteristic cf gastrointestinal (gi) manifestations including slowed transit and significant upregulation of genes associated with inflammation. to determine if characteristics of the microbiome are associate ...201723060053
evaluation of potential environmental contamination sources for the presence of multidrug-resistant bacteria linked to wound infections in combat casualties.to determine whether multidrug-resistant (mdr) gram-negative organisms are present in afghanistan or iraq soil samples, contaminate standard deployed hospital or modular operating rooms (ors), or aerosolize during surgical procedures.201222869264
oxidative stress response in atrazine-degrading bacteria exposed to atrazine.rhodobacter sphaeroides w16 and acinetobacter lwoffii dns32 which were isolated from soil in cold area subjected to a long-term atrazine application in heilongjiang province (china) can degrade atrazine efficiently. the investigation of their antioxidant properties will be useful for bioremediation and engineering applications of atrazine-degrading bacteria. superoxide dismutase (sod) and catalase (cat) from two atrazine-degrading bacteria and one non-atrazine-degrading bacterium were tested for ...201222704773
bacterial biodegradation of crude oil using local isolates.an experimental study was undertaken to assess the efficiency of pseudomonas aeruginosa, bacillus subtilis, and acinetobacter lwoffi isolated from petroleum contaminated water and soil samples to degrade crude oil, separately and in a mixed bacterial consortium. capillary gas chromatography was used for testing the effect of those bacterial species on the biodegradation of crude oil. individual bacterial cultures showed less growth and degradation than did the mixed bacterial consortium. at temp ...201426904742
arsenic-resistant and plant growth promoting firmicutes and γ-proteobacteria species from industrially polluted irrigation water and corresponding cropland.the aim of the study was to explore irrigation water polluted with industrial waste and corresponding cropland to screen bacteria for as-detoxification and plant growth promotion.201728708308
effects of multi-walled carbon nanotubes with various diameters on bacterial cellular membranes: cytotoxicity and adaptive mechanisms.the effect of multi-walled carbon nanotubes (mwnts) with different diameters on the destruction degree toward cellular membranes of bacterial has been explored by investigating the viability of bacteria and the change of composition and surface properties in cellular membranes with the exposure of mwnts. the atrazine degrading bacteria acinetobacter lwoffii dns32 (dns32) is chosen as the model species and escherichia coli (e. coli) and bacillus subtilis (b. subtilis) are selected as the comparis ...201728692883
acute blindness in a dog with acinetobacter-associated postencephalitic hydrocephalus.a 10-month-old male welsh corgi with a history of acute blindness underwent neuro-ophthalmological testing and magnetic resonance imaging (mri). vision testing revealed complete visual deficits but the electroretinograph and pupillary light reflex were normal in both eyes. the motor and sensory functions of the eyelids and eyes were also normal. the mri revealed compression of the optic chiasm caused by severe ventriculomegaly in the lateral and third ventricles. such lesions are associated with ...201728855429
acinetobacter lwoffii peritonitis in a patient on automated peritoneal dialysis: a case report and review of the literature.acinetobacter lwoffii, a nonfermentative gram-negative aerobic bacillus, which presents in the normal flora of the oropharynx and skin, has recently been reported as a cause of human infection. herein, the authors present a case report of peritonitis related to automated peritoneal dialysis caused by a. lwoffii.201728815095
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