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characterization of four viral species belonging to the family potyviridae isolated from ranunculus asiaticus.abstract four different viral species were isolated from diseased ranunculus asiaticus plants growing in imperia province (italian riviera-liguria region). infected plants exhibited mosaic symptoms and growth abnormalities. the viruses were mechanically inoculated to a range of herbaceous hosts and differentiated biologically. long flexuous particles were present in leaf dip extracts observed by electron microscopy. a general protocol for the amplification of potyvirus genome fragments through r ...200618943173
transmission efficiency of papaya ringspot virus by three aphid species.the transmission efficiency of papaya ringspot virus (prsv) by three aphid vectors (i.e., aphis gossypii, a. craccivora, and myzus persicae) was studied. efficiency was measured by single-aphid inoculation, group inoculation (using five aphids), duration of virus retention, and the number of plants following a single acquisition access period (aap) to which the aphids could successfully transmit the virus. single-aphid inoculation studies indicated that m. persicae (56%) and a. gossypii (53%) we ...200818943221
host range and characterization of sunflower mosaic virus.abstract sunflower mosaic is caused by a putative member of the family potyviridae. sunflower mosaic virus (sumv) was characterized in terms of host range, physical and biological characteristics, and partial nucleotide and amino acid sequence. cells infected with sumv had cytoplasmic inclusion bodies typical of potyviruses. of 74 genera tested, only species in helianthus, sanvitalia, and zinnia, all asteraceae, were systemic hosts. commercial sunflower hybrids from the united states, europe, an ...200218943264
transmission efficiency of cucumber mosaic virus by aphids associated with virus epidemics in snap bean.cucumber mosaic virus (cmv) is a major component of the virus complex that has become more pronounced in snap bean in the midwestern and northeastern united states since 2001. multiple-vector-transfer tests were done to estimate the cmv transmission efficiencies (p) of the main aphid species identified in commercial snap bean fields in new york and pennsylvania. the four most efficient vectors (p > 0.05) were aphis gossypii, a. glycines, acyrthosiphon pisum, and therioaphis trifolii, which were ...200818943413
hairy nightshade as a potential potato leafroll virus (luteoviridae: polerovirus) inoculum source in pacific northwest potato ecosystems.hairy nightshade, solanum sarrachoides, is a solanaceous weed found abundantly in pacific northwest potato ecosystems. it serves as a reservoir for one of the important potato viruses, potato leafroll virus (plrv) (luteoviridae: polerovirus), and its most important vector, the green peach aphid, myzus persicae (homoptera: aphididae). laboratory research indicated an increased green peach aphid settling and performance on s. sarrachoides than on potato. it also revealed that green peach aphids tr ...200818943736
identification and characterization of a novel whitefly-transmitted member of the family potyviridae isolated from cucurbits in florida.abstract a novel whitefly-transmitted member of the family potyviridae was isolated from a squash plant (cucurbita pepo) with vein yellowing symptoms in florida. the virus, for which the name squash vein yellowing virus (sqvyv) is proposed, has flexuous rod-shaped particles of approximately 840 nm in length. the experimental host range was limited to species in the family cucurbitaceae, with the most dramatic symptoms observed in squash and watermelon, but excluded all tested species in the fami ...200718944369
aphid acquisition and cellular transport of potato leafroll virus-like particles lacking p5 readthrough protein.abstract lepidopteran cells (spodoptera frugiperda) produced isometric virus-like particles (vlp) when infected with a recombinant baculovirus ac61 that contained the potato leafroll virus (plrv) coat protein gene modified with an n-terminal histidine tag (p3-6h). cells infected with acfl, a recombinant baculovirus that expressed cdna copies of the plrv genome rna, did not produce virus-like particles (vlp). in cell lines doubly infected with ac61 and acfl, vlp were formed that contained plrv-rn ...200018944480
binding of tomato spotted wilt virus to a 94-kda thrips protein.abstract using protein blot assays, a 94-kda thrips protein was identified that exhibited specific binding to tomato spotted wilt virus (tswv) particles. renaturation of the 94-kda protein, which is conserved among the two major vector species of tswv, frankliniella occidentalis and thrips tabaci, was crucial for its virus-binding properties, whereas under the same conditions no specific binding was observed with aphid (myzus persicae) proteins. the 94-kda protein species was present in all deve ...199818945001
pathogenic fungi and parasitoids of aphids present in air captures of migratory alates in the low-latitude plateau of yunnan, china.to survey fungal pathogens and parasitoids of aphids in the low-latitude plateau of yunnan, southwest china, 3,553 migratory alates of brevicoryne brassicae, lipaphis erysimi, and myzus persicae were attracted to a yellow-plus-plant trap from air during a full-year period and individually reared on cabbage leaves for 7-14 d at 18-22 degrees c and l:d 12:12. among the trapped alates, 19.2% were killed by 10 species of aphid-pathogenic fungi after survival of 2.3 d (range, 1-7 d). another 2.8% wer ...200819036206
testing nicotine tolerance in aphids using an artificial diet experiment.plants may upregulate the production of many different seconday metabolites in response to insect feeding. one of these metabolites, nicotine, is well know to have insecticidal properties. one response of tobacco plants to herbivory, or being gnawed upon by insects, is to increase the production of this neurotoxic alkaloid. here, we will demonstrate how to set up an experiment to address this question of whether a tobacco-adapted strain of the green peach aphid, myzus persicae, can tolerate high ...200819066588
testing the physiological barriers to viral transmission in aphids using microinjection.potato loafroll virus (plrv), from the family luteoviridae infects solanaceous plants. it is transmitted by aphids, primarily, the green peach aphid. when an uninfected aphid feeds on an infected plant it contracts the virus through the plant phloem. once ingested, the virus must pass from the insect gut to the hemolymph (the insect blood ) and then must pass through the salivary gland, in order to be transmitted back to a new plant. an aphid may take up different viruses when munching on a plan ...200819066589
investigation of carbohydrate binding property of a fungal lectin from xerocomus chrysenteron and potential use on myzus persicae aphid.in recent years encoding insecticidal lectins have been suggested as one of the promising methods against insect pests and have been engineered successfully into a variety of crops including wheat, rice, tobacco and potatoes. xerocomus chrysenteron lectin (xcl) has a high hemagglutinating activity and results obtained from sugar specificity assay showed to have specific affinity to galactose and n-acetylgalactosamine (galnac). in previous studies, xcl was shown to have negative effects on some i ...200819226804
integrating chemical and biological control of european corn borer in bell pepper.using multiple locations and a series of field trials over 2 yr, we evaluated an integrated pest management program for ostrinia nubilalis (hübner) (lepidoptera: crambidae) management in peppers involving biorational chemistries, inundative releases of trichogramma ostriniae (pang & chen), and conservation of generalist predators. in small plot trials, three biorational insecticides (spinosad, indoxacarb, and methoxyfenozide) provided comparable control of o. nubilalis as two broad-spectrum conv ...200919253647
a comparison of alternative plant mixes for conservation bio-control by native beneficial arthropods in vegetable cropping systems in queensland australia.cucurbit crops host a range of serious sap-sucking insect pests, including silverleaf whitefly (slw) and aphids, which potentially represent considerable risk to the australian horticulture industry. these pests are extremely polyphagous with a wide host range. chemical control is made difficult due to resistance and pollution, and other side-effects are associated with insecticide use. consequently, there is much interest in maximising the role of biological control in the management of these s ...201019323854
a simple, rapid and inexpensive method for localization of tomato yellow leaf curl virus and potato leafroll virus in plant and insect vectors.a simple, rapid, inexpensive method for the localization of virus transcripts in plant and insect vector tissues is reported here. the method based on fluorescent in situ hybridization using short dna oligonucleotides complementary to an rna segment representing a virus transcript in the infected plant or insect vector. the dna probe harbors a fluorescent molecule at its 5' or 3' ends. the protocol: simple fixation, hybridization, minimal washing and confocal microscopy, provides a highly specif ...200919406154
tracking the global dispersal of a cosmopolitan insect pest, the peach potato aphid.global commerce and human transportation are responsible for the range expansion of various insect pests such as the plant sucking aphids. high resolution dna markers provide the opportunity to examine the genetic structure of aphid populations, identify aphid genotypes and infer their evolutionary history and routes of expansion which is of value in developing management strategies. one of the most widespread aphid species is the peach-potato aphid myzus persicae, which is considered as a serio ...200919432979
influence of the potato leafroll virus and virus-infected plants on the arrestment of the aphid, myzus persicae.a series of experiments was conducted using membrane sachets containing mp148 diet or phosphate-buffered sucrose with and without purified potato leafroll virus to determine if direct encounter with the virus would arrest the aphid, myzus persicae (sulzer) (homoptera: aphididae). in only two out of 36 tests were there significantly more aphids settled on sachets containing the virus. in all other tests, there were either significantly fewer aphids on sachets containing virus or there were no dif ...200619537970
esterase-based resistance in the tobacco-adapted form of the green peach aphid, myzus persicae (sulzer) (hemiptera: aphididae) in the eastern united states.organophosphates and carbamates represent alternative insecticides in managing the tobacco-adapted form of the green peach aphid (tgpa), myzus persicae (sulzer), a major pest of tobacco in the united states and around the world. general esterases that detoxify these insecticides were assessed in green, red, and orange morphs of field-collected m. persicae. a total of 136 aphid colonies were collected from 2004 though 2007 and screened for total esterase activity. the green morphs had lower ester ...200919557739
quantitative estimation of plum pox virus targets acquired and transmitted by a single myzus persicae.the viral charge acquired and inoculated by single aphids in a non-circulative transmission is estimated using plum pox virus (ppv). a combination of electrical penetration graph and taqman real-time rt-pcr techniques was used to establish the average number of ppv rna targets inoculated by an aphid in a single probe (26,750), approximately half of the acquired ones. this number of ppv targets is responsible for a systemic infection of 20% on the inoculated receptor plants. no significant differ ...200919597934
plants under attack: multiple interactions with insects and microbes.to defend themselves, plants activate inducible defense mechanisms that are effective against the invader that is encountered. there is partial overlap in the defense signaling pathways that are induced by insect herbivores and microbial pathogens that may result in cross-resistance. we have previously shown that infestation by tissue-chewing pieris rapae larvae induces resistance in arabidopsis thaliana against subsequent attack by the microbial pathogens pseudomonas syringae pv. tomato (pst), ...200719704549
effect of commercially available plant-derived essential oil products on arthropod pests.plant-derived essential oil products, in general, are considered minimum-risk pesticides and are exempt from environmental protection agency registration under section 25(b) of the federal insecticide fungicide and rodenticide act. however, many of the plant-derived essential products available to consumers (homeowners) have not been judiciously evaluated for both efficacy and plant safety. in fact, numerous plant-derived essential oil products labeled for control of arthropod pests have not bee ...200919736770
complementation analysis of triple gene block of potato virus s (pvs) revealed its capability to support systemic infection and aphid transmissibility of recombinant potato virus x.triple gene block (tgb) sequences derived from isolates of ordinary potato virus s (pvs-o) and chenopodium-systemic (pvs-cs) were analyzed. although the tgb sequences did not reveal any specific difference within the 7k protein, some specific differences within the 25k and 12k orfs were found. in order to investigate a possible functional divergence of pvs-o and pvs-cs tgb variants, these genes were propagated in chimeric potato virus x (pvx). both pvs tgb variants partly complemented pvx tgb in ...200919748533
a strain of the bacterial symbiont regiella insecticola protects aphids against parasitoids.aphids commonly harbour facultative bacterial endosymbionts and may benefit from their presence through increased resistance to parasitoids. this has been demonstrated for hamiltonella defensa and serratia symbiotica, while a third common endosymbiont, regiella insecticola, did not provide such protection. however, this symbiont was recently detected in a highly resistant clone of the peach-potato aphid, myzus persicae, from australia. to test if resistance was indeed conferred by the endosymbio ...201019776066
parasitized aphids are inferior prey for a coccinellid predator: implications for intraguild predation.discussions of intraguild predation (igp) have assumed that the nutritional quality of intraguild (ig) prey is similar to that of any other prey available to the ig predator. it has been suggested therefore that generalist predators do not distinguish between healthy and parasitized aphids and thus function as facultative predators of parasitoids. more recent studies have shown that predators may selectively ingest prey of various nutritional qualities and that predators may have higher body nit ...200919791609
specific loops d, e and f of nicotinic acetylcholine receptor beta1 subunit may confer imidacloprid selectivity between myzus persicae and its predatory enemy pardosa pseudoannulata.one nicotinic acetylcholine receptor non-alpha subunit was cloned from the pond wolf spider, pardosa pseudoannulata, an important predatory enemy of some insect pests with agricultural importance, such as the green peach aphid myzus persicae. the subunit shows high amino acid identities to insect beta1 subunits (74-78%), and was denoted as ppbeta1. although high identities are found between ppbeta1 and insect beta1 subunits, amino acid differences are found within loops d, e and f, important seg ...200919818849
changes in green peach aphid responses to potato leafroll virus-induced volatiles emitted during disease progression.previous research has shown that green peach aphids, myzus persicae (sulzer), preferentially settle on leaflets of potato plants (solanum tuberosum l.) infected with potato leafroll virus (plrv) compared with sham-inoculated controls, at least in part because of aphid responses to volatile cues from the plants. the prior work used plants 4 wk after inoculation. in this study, aphid emigration from the vicinity of leaflets of plrv-infected plants at 2, 4, 6, 8, and 10 wk after inoculation was com ...200919825298
capsid protein of cowpea chlorotic mottle virus is a determinant for vector transmission by a beetle.cowpea chlorotic mottle virus (ccmv) is a bromovirus transmitted by species of chrysomelid beetles, including the spotted cucumber beetle, diabrotica undecimpunctata howardii barber. an experimental system was set up to identify the viral determinant(s) of the beetle transmission of ccmv. nicotiana clevelandii was selected as an experimental plant host because it supports the replication and accumulation of both ccmv and a second member of the family bromoviridae, cucumber mosaic virus (cmv). us ...201019828763
design, synthesis and biological activity of new strobilurin derivatives with a 1h-pyrazol-5-oxy side chain.a series of new strobilurin derivatives with a substituted pyrazole side chain (5 and 6) were designed and synthesized. the new derivatives were synthesized from substituted phenones as starting material via esterification, cyclization and condensation reactions. all compounds were identified by 1h nmr and ir spectral and elemental analyses. preliminary biological evaluation showed that some of the compounds had good fungicidal activity against cucumber downy mildew (cdm) and wheat powdery milde ...200919831032
reproductive performance of asexual clones of the peach-potato aphid, ( myzus persicae, homoptera: aphididae), colonising scotland in relation to host plant and field ecology.the population of peach-potato aphid, myzus persicae (sulzer), in scotland comprises large numbers of a few superclones with much smaller numbers of other clones, and the reason for their differential success has yet to be elucidated. in the current study, the reproduction of lineages derived from these clones was measured by counting the numbers of offspring produced by a one-day-old nymph after 15 days. this was measured on four plant species, including local agricultural hosts and at two diff ...201019941675
metabolic enzyme(s) confer imidacloprid resistance in a clone of myzus persicae (sulzer) (hemiptera: aphididae) from greece.previous studies have reported varying levels of resistance against imidacloprid in several insect species, including populations of the peach-potato aphid, myzus persicae (sulzer). these cases of resistance have been attributed to either target-site resistance or enhanced detoxification. in this study, a clone of m. persicae originating from greece revealed a 60-fold resistance factor to imidacloprid.201019950404
construction of beauveria bassiana t-dna insertion mutant collections and identification of thermosensitive and osmosensitive mutants.this study was to identify genes involved in thermotolerance and osmotolerance of beauveria bassiana.200920069875
deceptive chemical signals induced by a plant virus attract insect vectors to inferior hosts.previous studies have shown that vector-borne pathogens can alter the phenotypes of their hosts and vectors in ways that influence the frequency and nature of interactions between them, with significant implications for the transmission and spread of disease. for insect-borne pathogens, host odors are particularly likely targets for manipulation, because both plant- and animal-feeding insects use volatile compounds derived from their hosts as key foraging cues. here, we document the effects of a ...201020133719
evaluation of the biocontrol potential of various metarhizium isolates against green peach aphid myzus persicae (homoptera: aphididae).twenty-three isolates of metarhizium anisopliae (metschnikof) sorokin and m. acridum (driver & milner) jf bischoff, rehner & humber from non-aphid host insects around the globe were evaluated for their aphid biocontrol potential, which is not well known.201020201034
a peptide that binds the pea aphid gut impedes entry of pea enation mosaic virus into the aphid hemocoel.development of ways to block virus transmission by aphids could lead to novel and broad-spectrum means of controlling plant viruses. viruses in the luteoviridae enhanced are obligately transmitted by aphids in a persistent manner that requires virion accumulation in the aphid hemocoel. to enter the hemocoel, the virion must bind and traverse the aphid gut epithelium. by screening a phage display library, we identified a 12-residue gut binding peptide (gbp3.1) that binds to the midgut and hindgut ...201020223498
monitoring for imidacloprid resistance in the tobacco-adapted form of the green peach aphid, myzus persicae (sulzer) (hemiptera: aphididae), in the eastern united states.imidacloprid is the primary insecticide for controlling the tobacco-adapted form of the green peach aphid (tgpa), myzus persicae (sulzer), a major pest of tobacco worldwide. this study used leaf-dip bioassays to assess tgpa resistance to imidacloprid in the eastern united states from 2004 through 2007.201020309995
evaluation of systemic insecticides for green peach aphid control on tobacco. 197120333851
relation of aphicidal activity with cuticular degradation by beauveria bassiana sfb-205 supernatant incorporated with polyoxyethylene-(3)-isotridecyl ether.the application of beauveria bassiana sfb-205 supernatant incorporated with polyoxyethylene-(3)-isotridecyl ether (tde-3) significantly reduced the population of two species of aphids including cotton aphid, aphis gossypii, and green peach aphid, myzus persicae, much higher in cotton aphid, compared with supernatant incorporated with tween 80, which allows the relationship of aphicidal activity with the degradation of aphid cuticles to be determined. overall, the degradation of the cuticles indu ...201020372019
binding properties of the n-acetylglucosamine and high-mannose n-glycan pp2-a1 phloem lectin in arabidopsis.phloem protein2 (pp2) is a component of the phloem protein bodies found in sieve elements. we describe here the lectin properties of the arabidopsis (arabidopsis thaliana) pp2-a1. using a recombinant protein produced in escherichia coli, we demonstrated binding to n-acetylglucosamine oligomers. glycan array screening showed that pp2-a1 also bound to high-mannose n-glycans and 9-acyl-n-acetylneuraminic sialic acid. fluorescence spectroscopy-based titration experiments revealed that pp2-a1 had two ...201020442276
comparative analysis of the acyrthosiphon pisum genome and expressed sequence tag-based gene sets from other aphid species.to study gene repertoires and their evolution within aphids, we compared the complete genome sequence of acyrthosiphon pisum (reference gene set) and expressed sequence tag (est) data from three other species: myzus persicae, aphis gossypii and toxoptera citricida. we assembled ests, predicted coding sequences, and identified potential pairs of orthologues (reciprocical best hits) with a. pisum. pairwise comparisons show that a fraction of the genes evolve fast (high ratio of non-synonymous to s ...201020482638
induced defense by bemisia tabaci biotype b (hemiptera: aleyrodidae) in tobacco against myzus persicae (hemiptera: aphididae).myzus persicae and bemisia tabaci are serious pests of tobacco and can occur simultaneously on the same plant. we found that tobacco plants infested by whiteflies had fewer aphids than those without whiteflies. to determine whether b. tabaci feeding could induce plant defense against aphids locally and systemically, we determined the effects of b. tabaci on several biological parameters of m. persicae on tobacco. infestation of b. tabaci nymphs reduced survival rates of m. persicae by 30.0%. in ...201020550802
beet poleroviruses: close friends or distant relatives?summary taxonomy: there are three members of the genus polerovirus (family luteoviridae) that induce yellowing of sugar beet: beet mild yellowing virus (bmyv), beet chlorosis virus (bchv) and beet western yellows virus-usa (bwyv-usa, fig. 1). non-beet-infecting isolates of bwyv found particularly within europe have now been re-named turnip yellows virus (tuyv). species-specific antibodies are unavailable, but the viruses can be distinguished by rt-pcr using primers specifically designed to the 5 ...200520565633
turnip mosaic virus and the quest for durable resistance.summary taxonomy: turnip mosaic virus (tumv) is a member of the genus potyvirus (type species potato virus y) in the family potyviridae. to date, tumv is the only potyvirus known to infect brassicas. there are potyvirus isolates that appear serologically similar to tumv when tested with polyclonal antisera that do not readily infect brassicas (lesemann and vetten, 1985). physical properties: virions are approximately 720 x 15-20 nm flexuous rods (fig. 1) and are composed of 95% coat protein (cp) ...200220569337
elevated co2 shifts the focus of tobacco plant defences from cucumber mosaic virus to the green peach aphid.elevation in co(2) concentration broadly impacts plant physiological characteristics, which influences herbivores and biotrophic pathogens, which in turn regulate the plant defensive response. in this study, responses of tobacco plants to stress in the form of the green peach aphid, myzus persicae (sulzer), or cucumber mosaic virus (cmv), or both aphid and cmv combined were investigated in open-top chambers under ambient and elevated co(2) concentrations. we measured aboveground biomass and foli ...201020626646
the helper-component protease transmission factor of tobacco etch potyvirus binds specifically to an aphid ribosomal protein homologous to the laminin receptor precursor.potyviruses are plant pathogens transmitted by aphids in a non-persistent manner. during transmission, the virus-encoded factor helper-component protease (hcpro) is presumed to act as a molecular bridge, mediating the reversible retention of virions to uncharacterized binding sites in the vector mouthparts. whilst the predicted interaction between hcpro and the coat protein (cp) of virions has been confirmed experimentally, the characterization of putative hcpro-specific receptors in aphids has ...201020631085
uv-b impact on aphid performance mediated by plant quality and plant changes induced by aphids.plants face various abiotic and biotic environmental factors and therefore need to adjust their phenotypic traits on several levels. uv-b radiation is believed to impact herbivorous insects via host plant changes. plant responses to abiotic challenges (uv-b radiation) and their interaction with two aphid species were explored in a multifactor approach. broccoli plants [brassica oleracea l. convar. botrytis (l.), brassicaceae] were grown in two differently covered greenhouses, transmitting either ...201020636911
fungi associated with the hemlock woolly adelgid, adelges tsugae, and assessment of entomopathogenic isolates for management.fungi associated with the hemlock wooly adelgid, adelges tsugae annand (hemiptera: adelgidae), were collected throughout the eastern usa and southern china. twenty fungal genera were identified, as were 79 entomopathogenic isolates, including: lecanicillium lecanii (zimmermann) (hypocreales: insertae sedis), isaria farinosa (holm: fries.) (cordycipitaceae), beauveria bassiana (balasamo) (hyphomycetes), and fusarium spp (nectriaceae). the remaining fungal genera associated with insect cadavers we ...201020672977
thirty-seven transcription factor genes differentially respond to a harpin protein and affect resistance to the green peach aphid in arabidopsis.the harpin protein hrpn ea induces arabidopsis resistance to the green peach aphid by activating the ethylene signalling pathway and by recruiting ein2, an essential regulator of ethylene signalling, for a defence response in the plant. we investigated 37 ethylene-inducible arabidopsis transcription factor genes for their effects on the activation of ethylene signalling and insect defence. twenty-eight of the 37 genes responded to both ethylene and hrpn ea, and showed either increased or inhibit ...201020826953
biological characterization and complete genomic sequence of apium virus y infecting celery.a celery isolate of apium virus y (apvy-ce) from diseased plants in a commercial field in california was characterized. the experimental host range of the virus included 13 plant species in the families apiaceae, chenopodiaceae and solanaceae. almost all infected plant species showed foliar chlorosis and distortion or severe stunting and systemic chlorosis. apvy-ce was transmitted to all 10 host species in the apiaceae by green peach aphids. it reacted with the potyvirus group antibody and celer ...201120833213
effects of manduca sexta allatostatin and an analogue on the peach-potato aphid myzus persicae (hemiptera: aphididae) and degradation by enzymes in the aphid gut.the oral toxicity of the c-type allatostatin, manduca sexta allatostatin (manse-as) and the analogue δr³δr⁵manse-as, where r residues were replaced by their d-isomers, were tested against the peach-potato aphid myzus persicae by incorporation into an artificial diet. both peptides had significant dose-dependent effects on mortality, growth, and fecundity compared with control insects. the analogue, δr³δr⁵manse-as, had an estimated lc₅₀ of 0.31 µg/µl diet and was more potent than manse-as (estima ...201020936640
flufenerim, a novel insecticide acting on diverse insect pests: biological mode of action and biochemical aspects.a new chemical compound was tested for its insecticidal activity against several major insect pests. the compound, called "flufenerim", has a core pyrimidine structure and an unknown mode of action and showed potent activity against the sweet potato whitefly bemisia tabaci (gennadius), the green peach aphid myzus persicae (sulzer), and the african cotton leafworm spodoptera littoralis (boisduval); however, it did not show any activity against two thrips species: western flower thrips frankliniel ...201020958045
the invasion route for an insect pest species: the tobacco aphid in the new world.biological invasions are rapid evolutionary events in which populations are usually subject to a founder event during introduction followed by rapid adaptation to the new environment. molecular tools and bayesian approaches have shown their utility in exploring different evolutionary scenarios regarding the invasion routes of introduced species. we examined the situation for the tobacco aphid, myzus persicae nicotianae, a recently introduced aphid species in chile. using seven microsatellite loc ...201020958814
bt-toxin uptake by the non-target herbivore, myzus persicae (hemiptera: aphididae), feeding on transgenic oilseed rape in laboratory conditions.the potential non-target effects of genetically modified crops are some of the more debated topics within applied biotechnologies in agriculture and environmental risk assessment. the objective of the present research was to study the potential bt-toxin uptake by the non-target herbivore myzus persicae sulzer (hemiptera: aphididae) feeding on transgenic oilseed rape plants (brassica napus cv. 'westar' lines gt 2-4) expressing the cry1ac endotoxin. a specific aim was to replicate our previous exp ...201021034523
resistance of wild solanum accessions to aphids and other potato pests in quebec field conditions.two experiments were done to determine the susceptibility of six wild potato accessions to the aphids macrosiphum euphorbiae (thomas) (hemiptera: aphididae) and myzus persicae (sulzer). densities of aphid colonies were compared between caged solanum pinnatisectum dunal (solanales: solanaceae), s. polyadenium greenmam, s. tarijense hawkes, s. infundibuliforme philippi, s. oplocense hawkes, and s. stoloniferum schlechted and bouché, and the commercially cultivated potato plant s. tuberosum l. cv. ...201021054161
release of hormones from conjugates: chloroplast expression of β-glucosidase results in elevated phytohormone levels associated with significant increase in biomass and protection from aphids or whiteflies conferred by sucrose esters.transplastomic tobacco (nicotiana tabacum) plants expressing β-glucosidase (bgl-1) show modified development. they flower 1 month earlier with an increase in biomass (1.9-fold), height (1.5-fold), and leaf area (1.6-fold) than untransformed plants. trichome density on the upper and lower leaf surfaces of bgl-1 plants increase by 10- and 7-fold, respectively, harboring 5-fold more glandular trichomes (as determined by rhodamine b staining), suggesting that bgl-1 lines produce more sugar esters th ...201021068365
a functional genomics approach identifies candidate effectors from the aphid species myzus persicae (green peach aphid).aphids are amongst the most devastating sap-feeding insects of plants. like most plant parasites, aphids require intimate associations with their host plants to gain access to nutrients. aphid feeding induces responses such as clogging of phloem sieve elements and callose formation, which are suppressed by unknown molecules, probably proteins, in aphid saliva. therefore, it is likely that aphids, like plant pathogens, deliver proteins (effectors) inside their hosts to modulate host cell processe ...201021124944
toward a mechanistic understanding of competition in vascular-feeding herbivores: an empirical test of the sink competition hypothesis.recent evidence suggests that competitive interactions among herbivores are mostly indirect and mediated by plant responses to herbivory. most studies, however, emphasize chewing insects and secondary chemistry, thus ignoring the diverse group of vascular-parasites that may be more likely to compete through induced changes in phytonutrients. using an aboveground phloem-feeding aphid (myzus persicae) and a belowground gall-forming nematode (meloidogyne incognita) on tobacco plants, we assessed th ...201021181415
insecticidal activity of rhamnolipid isolated from pseudomonas sp. ep-3 against green peach aphid (myzus persicae).microorganisms capable of growth on oils are potential sources of biopesticides, as they produce complex molecules such as biosurfactants and lipopeptides. these molecules have antimicrobial activity against plant pathogens, but few data are available on their insecticidal activity. the present study describes the insecticidal activity of a rhamnolipid isolated from diesel oil-degrading pseudomonas sp. ep-3 (ep-3). the treatment of cell-free supernatants of ep-3 grown on glucose-mineral medium f ...201021192722
harpin-induced expression and transgenic overexpression of the phloem protein gene atpp2-a1 in arabidopsis repress phloem feeding of the green peach aphid myzus persicae.treatment of plants with hrpnea, a protein of harpin group produced by gram-negative plant pathogenic bacteria, induces plant resistance to insect herbivores, including the green peach aphid myzus persicae, a generalist phloem-feeding insect. under attacks by phloem-feeding insects, plants defend themselves using the phloem-based defense mechanism, which is supposed to involve the phloem protein 2 (pp2), one of the most abundant proteins in the phloem sap. the purpose of this study was to obtain ...201121226963
molecular, ultrastructural, and biological characterization of pennsylvania isolates of plum pox virus.plum pox virus (ppv) was identified in pennsylvania in 1999. the outbreak was limited to a four-county region in southern pennsylvania. initial serological and molecular characterization indicated that the isolates in pennsylvania belong to the d strain of ppv. the pennsylvania isolates were characterized by sequence analysis, electron microscopy, host range, and vector transmission to determine how these isolates related to their previously studied european counterparts. genetically, pennsylvan ...201121261466
novel nicotinic action of the sulfoximine insecticide sulfoxaflor.the novel sulfoximine insecticide sulfoxaflor is as potent or more effective than the neonicotinoids for toxicity to green peach aphids (gpa, myzus persicae). the action of sulfoxaflor was characterized at insect nicotinic acetylcholine receptors (nachrs) using electrophysiological and radioligand binding techniques. when tested for agonist properties on drosophila melanogaster da2 nachr subunit co-expressed in xenopus laevis oocytes with the chicken ß2 subunit, sulfoxaflor elicited very high am ...201121296156
controlling myzus persicae with recombinant endophytic fungi chaetomium globosum expressing pinellia ternata agglutinin: using recombinant endophytic fungi to control aphids.sap-sucking insect pests have become the major threats to many crops in recent years; however, only a few biopesticides have been developed for controlling those pests. here, we developed a novel pest management strategy, which uses endophytes to express anti-pest plant lectins.201121414115
hrpn ea-induced deterrent effect on phloem feeding of the green peach aphid myzus persicae requires atgsl5 and atmyb44 genes in arabidopsis thaliana.in arabidopsis thaliana (arabidopsis) treated with the harpin protein hrpn ea, resistance to the green peach aphid myzus persicae, a generalist phloem-feeding insect, develops with induced expression of the atmyb44 gene. special glucan synthesis-like (gsl) genes and beta-1,3-glucan callose play an important role in plant defence responses to attacks by phloem-feeding insects. here we report that atgls5 and atmyb44 are both required for hrp ea-induced repression of m. persicae feeding from the ph ...201121451254
overexpression of a harpin-encoding gene hrf1 in rice enhances drought tolerance.harpin proteins are well known as eliciters that induce multiple responses in plants, such as systemic acquired resistance, hypersensitive response, enhancement of growth, resistance to the green peach aphid, and tolerance to drought. overexpression of harpin-encoding genes enhances plant resistance to diseases in tobacco, rice, rape, and cotton; however, it is not yet known whether the expression of harpin-encoding genes in vivo improves plant tolerance to abiotic stresses. the results of this ...201121527628
the influence of virus-induced changes in plants on aphid vectors: insights from luteovirus pathosystems.plant virus infection can alter the suitability of host plants for their aphid vectors. most reports indicate that virus-infected plants are superior hosts for vectors compared to virus-free plants with respect to vector growth rates, fecundity and longevity. some aphid vectors respond preferentially to virus-infected plants compared to virus-free ones, while others avoid infected plants that are inferior hosts. thus, it appears vectors can exploit changes in host plant quality associated with v ...201121549769
minimising losses caused by zucchini yellow mosaic virus in vegetable cucurbit crops in tropical, sub-tropical and mediterranean environments through cultural methods and host resistance.between 2006 and 2009, 10 field experiments were done at kununurra, carnarvon or medina in western australia (wa) which have tropical, sub-tropical and mediterranean climates, respectively. these experiments investigated the effectiveness of cultural control measures in limiting zymv spread in pumpkin, and single-gene resistance in commercial cultivars of pumpkin, zucchini and cucumber. melon aphids (aphis gossypii) colonised field experiments at kununurra; migrant green peach aphids (myzus pers ...201121549770
antipredator behaviour of myzus persicae affects transmission efficiency of broad bean wilt virus 1.biological control has the potential to limit the population growth of arthropod vectors and consequently may be expected to reduce plant virus spread in a crop. however, reduction of vector abundance is not the only effect of biological control. natural enemies might induce antipredator behaviour that affects feeding and dispersal of vectors, and therefore virus spread. here we test the effect of two natural enemies on dispersal of the aphid vector myzus persicae and transmission of broad bean ...201121549771
transgenic tetraploid isatis indigotica expressing bt cry1ac and pinellia ternata agglutinin showed enhanced resistance to moths and aphids.co-expression of multiple genes encoding different kinds of insect resistant proteins has been developed to confer a broader spectrum of pest control. tetraploid isatis indigotica fort was transformed with a plasmid, p3300bp, containing bacillus thuringiensis cry1ac gene (bt) and pinellia ternata agglutinin gene (pta) and the selectable marker herbicide resistance gene (bar) driven by the camv35s promoter via agrobacterium tumefaciens-mediated transformation. the integration and expression of in ...201121559837
within plant distribution of potato virus y in hairy nightshade (solanum sarrachoides): an inoculum source affecting pvy aphid transmission.potato virus y (pvy) is vectored by several potato-colonizing and non-colonizing aphid species in a non-persistent manner and has a wide host range. it occurs naturally in several plant families. myzus persicae and macrosiphum euphorbiae are the most efficient potato-colonizing aphid vectors of pvy. rhopalosiphum padi, a cereal aphid that migrates in large numbers through potato fields during the middle of the growing season, does not colonize potato plants but can transmit pvy. hairy nightshade ...201121601597
aphids preserved in propylene glycol can be used for reverse transcription-polymerase chain reaction detection of potato virus y.the effectiveness of propylene glycol on the retention of rna target of potato virus y (pvy), an aphid stylet-borne virus, in myzus persicae was investigated in comparison to ethanol and liquid nitrogen/-80°c. reverse transcription-polymerase chain reaction (rt-pcr) was used to detect the pvy targets from the propylene glycol/ethanol/liquid nitrogen preserved single aphids after a 5min acquisition period from infected potato plants. in the liquid nitrogen/-80°c and 70% ethanol treatments, 55.6% ...201121621556
presence of a functional (ttagg)(n) telomere-telomerase system in aphids.the structure of the telomeres of four aphid species (acyrthosiphon pisum, megoura viciae, myzus persicae and rhopalosiphum padi) was evaluated by southern blotting and fluorescent in situ hybridization, revealing that each chromosomal end consists of a (ttagg)(n) repeat. the presence of a telomerase coding gene has been verified successively in the a. pisum genome, revealing that aphid telomerase shares sequence identity ranging from 12% to 18% with invertebrate and vertebrate homologues, and p ...201121667174
Biosynthesis and defensive function of Nd-acetylornithine, a jasmonate-induced Arabidopsis metabolite.Since research on plant interactions with herbivores and pathogens is often constrained by the analysis of already known compounds, there is a need to identify new defense-related plant metabolites. The uncommon nonprotein amino acid N(d)-acetylornithine was discovered in a targeted search for Arabidopsis thaliana metabolites that are strongly induced by the phytohormone methyl jasmonate (MeJA). Stable isotope labeling experiments show that, after MeJA elicitation, Arg, Pro, and Glu are converte ...201121917546
[effects of beauveria bassiana on myzus persicae and its two predaceous natural enemies].a beauveria bassiana strain bb21 was isolated from naturally infected green peach aphid myzus persicae (hemiptera: aphididae). the effects of the strain on m. persicae and its two predaceous natural enemies chrysoperla carnea (neuroptera: chrysopidae) and harmonia axyridis (coleoptera: coccinellidae) were investigated under laboratory conditions. bb21 had strong pathogenicity to m. persicae, with the ld50 of 97 conidia x mm(-2) (45-191, 95% confidence interval), but was less pathogenic to the se ...201122126056
pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, lepidopteran insects, bacterial and viral pathogens.broad spectrum protection against different insects and pathogens requires multigene engineering. however, such broad spectrum protection against biotic stress is provided by a single protein in some medicinal plants. therefore, tobacco chloroplasts were transformed with the agglutinin gene from pinellia ternata (pta), a widely cultivated chinese medicinal herb. pinellia ternata agglutinin (pta) was expressed up to 9.2% of total soluble protein in mature leaves. purified pta showed similar hemag ...201122077160
ecological responses to uv radiation: interactions between the biological effects of uv on plants and on associated organisms.solar ultraviolet (uv)-b radiation (280-315 nm) has a wide range of effects on terrestrial ecosystems, yet our understanding of how uv-b influences the complex interactions of plants with pest, pathogen and related microorganisms remains limited. here, we report the results of a series of experiments in lactuca sativa which aimed to characterize not only key plant responses to uv radiation in a field environment but also consequential effects for plant interactions with a sap-feeding insect, two ...201122150399
Assessing aphids potato virus Y-transmission efficiency: A new approach.In order to develop an alternative method to optimize the relative efficiency factor (REF) assessment, the efficiency of transmission of Potato virus Y (PVY) by seven aphid species was examined. In vitro micropropagated potato plantlets were used to experiment on phenotypically and genetically homogeneous material. Species-specific acquisition access period (AAP) on a PVY-infected plantlet was assessed for each aphid species using electrical penetration graph (EPG) technique. Aphid probing behav ...201121884729
purification of a new fungal mannose-specific lectin from penicillium chrysogenum and its aphicidal properties.several ascomycetes fungi are commonly used in bio-industries and provide available industrial residues for lectin extraction to be valuable. a lectin from penicillium chrysogenum, named pecl, was purified from a fungal culture using gel-filtration chromatography column. pecl was found to be a mannose-specific lectin by haemagglutination activity towards rabbit erythrocyte cells and was visualised on sds-page gel. purified pecl fraction was delivered via artificial diet to myzus persicae aphid a ...201122036288
(e)-β-farnesene synthase genes affect aphid (myzus persicae) infestation in tobacco (nicotiana tabacum).aphids are major agricultural pests which cause significant yield losses of the crop plants each year. (e)-β-farnesene (eβf) is the alarm pheromone involved in the chemical communication between aphids and particularly in the avoidance of predation. in the present study, two eβf synthase genes were isolated from sweet wormwood and designated as aaβfs1 and aaβfs2, respectively. overexpression of aaβfs1 or aaβfs2 in tobacco plants resulted in the emission of eβf ranging from 1.55 to 4.65 ng/day/g ...201121847661
Cage and field assessments of Beauveria bassiana-based Mycoinsecticides for Myzus persicae Sulzer (Hemiptera: Aphididae) control in cabbage.The efficiency of formulated Beauveria bassiana-based mycoinsecticides to control Myzus persicae (Sulzer) in cabbage was assessed under field conditions. Aqueous conidial suspensions (0.01% Tween 80 + 0.01% v/v Agral) of three fungal isolates were sprayed twice at different dates, each with 2.0 x 10(9) viable conidia per potted plant using screened cages. The number of nymphs and adults of M. persicae per leaf was significantly reduced in plots treated with isolates CG 864 and PL 63, with contro ...201121952964
cucumber mosaic virus and its 2b rna silencing suppressor modify plant-aphid interactions in tobacco.the cucumber mosaic virus (cmv) 2b protein not only inhibits anti-viral rna silencing but also quenches transcriptional responses of plant genes to jasmonic acid, a key signalling molecule in defence against insects. this suggested that it might affect interactions between infected plants and aphids, insects that transmit cmv. we found that infection of tobacco with a 2b gene deletion mutant (cmvδ2b) induced strong resistance to aphids (myzus persicae) while cmv infection fostered aphid survival ...201122355702
aphid feeding behavior in relation to potato virus y (pvy) acquisition.potato virus y (pvy) is transmitted by aphids in a nonpersistent manner and aphid species differ in their ability to transmit pvy. during host selection, aphids will land and probe on nonhost plant species and this behavior is an important component of the epidemiology of many aphid-transmitted viruses. in this study, we hypothesized that host selection behavior varies between aphid species and the host or nonhost status of the plant and this behavior will modulate their ability to acquire pvy. ...201223356052
effects of simulated heat waves on an experimental plant-herbivore-predator food chain.greater climatic variability and extreme climatic events are currently emerging as two of the most important facets of climate change. predicting the effects of extreme climatic events, such as heat waves, is a major challenge because they may affect both organisms and trophic interactions, leading to complex responses at the community level. in this study, we set up a simple three-level food chain composed of a sweet pepper plant, capsicum annuum; an aphid, myzus persicae; and a ladybeetle, col ...201223504840
an in vitro study of esterases, hydrolysing non-specific substrates, of an op-resistant strain of the green peach aphid, myzus persicae (sulz.). 20164714903
[ultrastructural aspects of the salivary glands in the aphid myzus persicae (suiz.) homoptera aphididae]. 20154463419
aphids alter host-plant nitrogen isotope fractionation.plant sap-feeding insects and blood-feeding parasites are frequently depleted in (15)n relative to their diet. unfortunately, most fluid-feeder/host nitrogen stable-isotope studies simply report stable-isotope signatures, but few attempt to elucidate the mechanism of isotopic trophic depletion. here we address this deficit by investigating the nitrogen stable-isotope dynamics of a fluid-feeding herbivore-host plant system: the green peach aphid, myzus persicae, feeding on multiple brassicaceous ...201121646532
water stress and aphid feeding differentially influence metabolite composition in arabidopsis thaliana (l.).little is known about how drought stress influences plant secondary metabolite accumulation and how this affects plant defense against different aphids. we therefore cultivated arabidopsis thaliana (l.) plants under well-watered, drought, and water-logged conditions. two aphid species were selected for this study: the generalist myzus persicae (sulzer) and the crucifer specialist brevicoryne brassicae (l.). metabolite concentrations in the phloem sap, which influence aphid growth, changed partic ...201223144921
arabidopsis thaliana-aphid interaction.aphids are important pests of plants that use their stylets to tap into the sieve elements to consume phloem sap. besides the removal of photosynthates, aphid infestation also alters source-sink patterns. most aphids also vector viral diseases. in this chapter, we will summarize on recent significant findings in plant-aphid interaction, and how studies involving arabidopsis thaliana and myzus persicae (sülzer), more commonly known as the green peach aphid (gpa), are beginning to provide importan ...201222666177
suppression of plant defenses by a myzus persicae (green peach aphid) salivary effector protein.the complex interactions between aphids and their host plant are species-specific and involve multiple layers of recognition and defense. aphid salivary proteins, which are released into the plant during phloem feeding, are a likely mediator of these interactions. in an approach to identify aphid effectors that facilitate feeding from host plants, eleven myzus persicae (green peach aphid) salivary proteins and the groel protein of buchnera aphidicola, a bacterial endosymbiont of this aphid speci ...201424654979
groel from the endosymbiont buchnera aphidicola betrays the aphid by triggering plant defense.aphids are sap-feeding plant pests and harbor the endosymbiont buchnera aphidicola, which is essential for their fecundity and survival. during plant penetration and feeding, aphids secrete saliva that contains proteins predicted to alter plant defenses and metabolism. plants recognize microbe-associated molecular patterns and induce pattern-triggered immunity (pti). no aphid-associated molecular pattern has yet been identified. by mass spectrometry, we identified in saliva from potato aphids (m ...201424927572
infection of host plants by cucumber mosaic virus increases the susceptibility of myzus persicae aphids to the parasitoid aphidius colemani.plant viruses can profoundly alter the phenotypes of their host plants, with potentially far-reaching implications for ecology. yet few studies have explored the indirect, host-mediated, effects of plant viruses on non-vector insects. we examined how infection of cucurbita pepo plants by cucumber mosaic virus (cmv) impacted the susceptibility of aphids (myzus persicae) to attack by the parasitoid wasp aphidius colemani. in semi-natural foraging assays, we observed higher rates of aphid parasitis ...201526043237
comparative analysis of rnai-based methods to down-regulate expression of two genes expressed at different levels in myzus persicae.with the increasing availability of aphid genomic data, it is necessary to develop robust functional validation methods to evaluate the role of specific aphid genes. this work represents the first study in which five different techniques, all based on rna interference and on oral acquisition of double-stranded rna (dsrna), were developed to silence two genes, aly and eph, potentially involved in polerovirus transmission by aphids. efficient silencing of only eph transcripts, which are less abund ...201627869783
nitrogen hurdle of host alternation for a polyphagous aphid and the associated changes of endosymbionts.low proportion of essential amino acids (eaas) is one of the barriers for animals to use phloem as a diet. endosymbionts with eaas synthesis functions are considered crucial for ameliorating the lack of eaas in insects' diets. in this study, we transferred the insects from a cabbage-reared myzus persicae population onto 3 new plant species including eggplant, tobacco and spinach. the performance on these plants was evaluated and the dynamics of endosymbionts in relation to this host alternation ...201627094934
two odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (e)-ß-farnesene and structural analogues.aphids are agricultural pests of great economical interest. alternatives to insecticides, using semiochemicals, are of difficult applications. in fact, sex pheromones are of little use as aphids reproduce partenogenetically most of the time. besides, the alarm pheromone, (e)-ß-farnesene for a great number of species, is difficult to synthesize and unstable in the environment. the search for novel semiochemicals to be used in population control can be efficiently approached through the study of t ...201222427877
resistance of arabidopsis thaliana to the green peach aphid, myzus persicae, involves camalexin and is regulated by micrornas.· small rnas play important roles in resistance to plant viruses and the complex responses against pathogens and leaf-chewing insects. · we investigated whether small rna pathways are involved in arabidopsis resistance against a phloem-feeding insect, the green peach aphid (myzus persicae). we used a 2-wk fecundity assay to assess aphid performance on arabidopsis rna silencing and defence pathway mutants. quantitative real-time polymerase chain reaction was used to monitor the transcriptional ac ...201323528052
the potato aphid salivary effector me47 is a glutathione-s-transferase involved in modifying plant responses to aphid infestation.polyphagous aphid pests cause considerable economic damage to crop plants, primarily through the depletion of photoassimilates and transfer of viruses. the potato aphid (macrosiphum euphorbiae) is a notable pest of solanaceous crops, however, the molecular mechanisms that underpin the ability to colonize these hosts are unknown. it has recently been demonstrated that like other aphid species, m. euphorbiae injects a battery of salivary proteins into host plants during feeding. it is speculated t ...201627536306
molecular responses to aphid feeding in arabidopsis in relation to plant defense pathways.little is known about molecular responses in plants to phloem feeding by insects. the induction of genes associated with wound and pathogen response pathways was investigated following green peach aphid (myzus persicae) feeding on arabidopsis. aphid feeding on rosette leaves induced transcription of two genes associated with salicylic acid (sa)-dependent responses to pathogens (pr-1 and bgl2) 10- and 23-fold, respectively. induction of pr-1 and bgl2 mrna was reduced in npr1 mutant plants, which ...200111161062
major signaling pathways modulate arabidopsis glucosinolate accumulation and response to both phloem-feeding and chewing insects.plant responses to enemies are coordinated by several interacting signaling systems. molecular and genetic studies with mutants and exogenous signal application suggest that jasmonate (ja)-, salicylate (sa)-, and ethylene (et)-mediated pathways modulate expression of portions of the defense phenotype in arabidopsis (arabidopsis thaliana), but have not yet linked these observations directly with plant responses to insect attack. we compared the glucosinolate (gs) profiles of rosette leaves of 4-w ...200515923339
dynamics of membrane potential variation and gene expression induced by spodoptera littoralis, myzus persicae, and pseudomonas syringae in arabidopsis.biotic stress induced by various herbivores and pathogens invokes plant responses involving different defense mechanisms. however, we do not know whether different biotic stresses share a common response or which signaling pathways are involved in responses to different biotic stresses. we investigated the common and specific responses of arabidopsis thaliana to three biotic stress agents: spodoptera littoralis, myzus persicae, and the pathogen pseudomonas syringae.201223118859
uv-b irradiation changes specifically the secondary metabolite profile in broccoli sprouts: induced signaling overlaps with defense response to biotic stressors.only a few environmental factors have such a pronounced effect on plant growth and development as ultraviolet light (uv). concerns have arisen due to increased uv-b radiation reaching the earth's surface as a result of stratospheric ozone depletion. ecologically relevant low to moderate uv-b doses (0.3-1 kj m(-2) d(-1)) were applied to sprouts of the important vegetable crop brassica oleracea var. italica (broccoli), and eco-physiological responses such as accumulation of non-volatile secondary ...201222773681
discrimination of arabidopsis pad4 activities in defense against green peach aphid and pathogens.the arabidopsis (arabidopsis thaliana) lipase-like protein phytoalexin deficient4 (pad4) is essential for defense against green peach aphid (gpa; myzus persicae) and the pathogens pseudomonas syringae and hyaloperonospora arabidopsidis. in basal resistance to virulent strains of p. syringae and h. arabidopsidis, pad4 functions together with its interacting partner enhanced disease susceptibility1 (eds1) to promote salicylic acid (sa)-dependent and sa-independent defenses. by contrast, dissociate ...201222353573
loss of function of fatty acid desaturase7 in tomato enhances basal aphid resistance in a salicylate-dependent manner.we report here that disruption of function of the ω-3 fatty acid desaturase7 (fad7) enhances plant defenses against aphids. the suppressor of prosystemin-mediated responses2 (spr2) mutation in tomato (solanum lycopersicum), which eliminates the function of fad7, reduces the settling behavior, survival, and fecundity of the potato aphid (macrosiphum euphorbiae). likewise, the antisense suppression of lefad7 expression in wild-type tomato plants reduces aphid infestations. aphid resistance in the ...201222291202
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