| rapid purification of soybean mosaic virus from small quantities of tissue by immunoprecipitation. | a rapid, simple, and efficient method for purification of soybean mosaic virus (smv) was developed based on immunoprecipitation. traditional centrifugation-based methods for purification of smv and other potyviruses require long, complicated procedures and large quantities of infected tissue (100-500 g). the immunoprecipitation procedure described in this study allows the purification of intact smv virion particles in 4h from 0.5 g of tissue. the reliability of this procedure was demonstrated by ... | 2013 | 23567843 |
| detection and evolutionary analysis of soybean mirnas responsive to soybean mosaic virus. | micrornas (mirna) are a class of non-coding rnas that have important gene regulatory roles in various organisms. however, the mirnas involved in soybean's response to soybean mosaic virus (smv) are unknown. to identify novel mirnas and biotic-stress regulated small rnas that are involved in soybean's response to smv, two small rna libraries were constructed from mock-inoculated and smv-infected soybean leaves and sequenced. this led to the discovery of 179 mirnas, representing 52 families, among ... | 2013 | 23328897 |
| overexpression of a soybean nuclear localized type-iii dnaj domain-containing hsp40 reveals its roles in cell death and disease resistance. | heat-shock proteins such as hsp70 and hsp90 are important molecular chaperones that play critical roles in biotic and abiotic stress responses; however, the involvement of their co-chaperones in stress biology remains largely uninvestigated. in a screen for candidate genes stimulating cell death in glycine max (soybean), we transiently overexpressed full-length cdnas of soybean genes that are highly induced during soybean rust infection in nicotiana benthamiana leaves. overexpression of a type-i ... | 2013 | 23289813 |
| the hc-pro and p3 cistrons of an avirulent soybean mosaic virus are recognized by different resistance genes at the complex rsv1 locus. | the complex rsv1 locus in soybean plant introduction (pi) 'pi96983' confers extreme resistance (er) against soybean mosaic virus (smv) strain n but not smv-g7 and smv-g7d. both the smv helper-component proteinase (hc-pro) and p3 cistrons can serve as avirulence factors recognized by rsv1. to understand the genetics underlying recognition of the two cistrons, we have utilized two soybean lines (l800 and l943) derived from crosses between pi96983 (rsv1) and lee68 (rsv1) with distinct recombination ... | 2013 | 23051173 |
| evaluation of north american isolates of soybean mosaic virus for gain of virulence on rsv-genotype soybeans with special emphasis on resistance-breaking determinants on rsv4. | resistance to soybean mosaic virus (smv) in soybean is conferred by three dominant genes: rsv1, rsv3 and rsv4. over the years, scientists in the usa have utilized a set of standard pathotypes, smv-g1 to smv-g7, to study interaction with rsv-genotype soybeans. however, these pathotypes were isolated from a collection of imported soybean germplasm over 30 years ago. in this study, 35 smv field isolates collected in recent years from 11 states were evaluated for gain of virulence on soybean genotyp ... | 2012 | 22827506 |
| the requirement of multiple defense genes in soybean rsv1-mediated extreme resistance to soybean mosaic virus. | soybean mosaic virus (smv) is a major viral pathogen of soybean. among the three smv resistance genes, rsv1 mediates extreme resistance (er) against most smv strains, including the β-glucuronidase-tagged g2 isolate that was previously used in studies of rsv1. using virus-induced gene silencing (vigs), we screened 82 vigs constructs to identify genes that play a role in rsv1-mediated er to smv infection. the target genes included putative rsv1 candidate genes, soybean orthologs to known defense-s ... | 2012 | 22712511 |
| ultrasensitive determination of jasmonic acid in plant tissues using high-performance liquid chromatography with fluorescence detection. | an ultrasensitive and selective high-performance liquid chromatographic method for the volatile signaling hormone, jasmonic acid, has been developed based on precolumn derivatization with 1,3,5,7-tetramethyl-8-aminozide-difluoroboradiaza-s-indacene (bodipy-aminozide). the derivatization reaction was carried out at 60 °c for 30 min in the presence of phosphoric acid. the formed jasmonic acid derivative was eluted using a mobile phase of methanol/ph 6.50 ammonium formate buffer/tetrahydrofuran (67 ... | 2012 | 22551211 |
| divergence and allelomorphic relationship of a soybean virus resistance gene based on tightly linked dna microsatellite and rflp markers. | the use of genetically diverse resistance sources is important in breeding for durable disease resistance. detection and evaluation of resistance genes by conventional inheritance experiments, however, often require laborious screening and genetic testing. in the present study, a marker-assisted screening for resistance sources was initiated in soybean [glycine max (l.) merr] using one dna microsatellite and two rflp markers tightly linked to a soybean mosaic virus (smv) resistance gene (rsv1). ... | 1996 | 24166117 |
| genetic characteristics of two genes for resistance to soybean mosaic virus in pi486355 soybean. | soybean [glycine max (l.) merr.] pi486355 is resistant to all the identified strains of soybean mosaic virus (smv) and possesses two independently inherited resistance genes. to characterize the two genes, pi486355 was crossed with the susceptible cultivars 'lee 68' and 'essex' and with cultivars 'ogden' and 'marshall', which are resistant to smv-g1 but systemically necrotic to smv-g7. the f2 populations and f2∶3 progenies from these crosses were inoculated with smv-g7 in the greenhouse. the two ... | 1995 | 24169976 |
| inheritance of resistance to potyviruses in phaseolus vulgaris l. ii. linkage relations and utility of a dominant gene for lethal systemic necrosis to soybean mosaic virus. | a single dominant factor, hss, that conditions a rapid lethal necrotic response to soybean mosaic virus (smv) has been identified in phaseolus vulgaris l. cv. 'black turtle soup', line bt-1. inoculated plants carrying this factor developed pinpoint necrotic lesions on inoculated tissue followed by systemic vascular necrosis and plant death within about 7 days, regardless of ambient temperature. bt-1 also carries dominant resistance to potyviruses attributed to the tightly linked or identical fac ... | 1993 | 24193459 |
| robust rnai-mediated resistance to infection of seven potyvirids in soybean expressing an intron hairpin nib rna. | viral pathogens, such as soybean mosaic virus (smv), are a major constraint in soybean production and often cause significant yield loss and quality deterioration. engineering resistance by rnai-mediated gene silencing is a powerful strategy for controlling viral diseases. in this study, a 248-bp inverted repeat of the replicase (nuclear inclusion b, nib) gene was isolated from the smv sc3 strain, driven by the leaf-specific rbcs2 promoter from phaseolus vulgaris, and introduced into soybean. th ... | 2017 | 28840434 |
| rnai-mediated smv p3 cistron silencing confers significantly enhanced resistance to multiple potyvirus strains and isolates in transgenic soybean. | robust rnai-mediated resistance to multiple potyvirus strains and isolates, but not to secovirus bpmv, was conferred by expressing a short smv p3 hairpin in soybean plants. engineering resistance to multiple potyvirus strains is of great interest because of a wide variability of the virus strains, and mixed infections of multiple viruses or strains commonly associated with field grown soybean. in this study, rnai-mediated silencing of the soybean mosaic virus (smv) p3 cistron, which is reported ... | 2017 | 28756582 |
| fine-mapping and identification of a novel locus rsc15 underlying soybean resistance to soybean mosaic virus. | rsc15, a novel locus underlying soybean resistance to smv, was fine mapped to a 95-kb region on chromosome 6. the rsc15- mediated resistance is likely attributed to the gene gmpex14 , the relative expression of which was highly correlated with the accumulation of h 2 o 2 along with the activities of pod and cat during the early stages of smv infection in rn-9. soybean mosaic virus (smv) causes severe yield losses and seed quality deterioration in soybean [glycine max (l.) merr.] worldwide. a ser ... | 2017 | 28825113 |
| downregulation of genes coding for core rnai components and disease resistance proteins via corresponding micrornas might be correlated with successful smv infection in soybean. | plants protect themselves from virus infections by several different defense mechanisms. rna interference (rnai) is one prominent antiviral mechanism, which requires the participation of ago (argonaute) and dicer/dcl (dicer-like) proteins. effector-triggered immunity (eti) is an antiviral mechanism mediated by resistance (r) genes, most of which encode nbs-lrr family proteins. micrornas (mirnas) play important regulatory roles in plants, including regulation of host defenses. smv (soybean mosaic ... | 2017 | 28695996 |
| over-expression of gmsn1 enhances virus resistance in arabidopsis and soybean. | gmsn1 enhances virus resistance in plants most likely by affecting the expression of signal transduction and immune response genes. soybean mosaic virus (smv) infection causes severe symptom and leads to massive yield loss in soybean (glycine max). by comparative analyzing gene expression in the smv-resistant soybean cultivar rsmv1 and the susceptible cultivar ssmv1 at a transcriptome level, we found that a subgroup of gibberellic acid stimulated transcript (gast) genes were down-regulated in sm ... | 2017 | 28656325 |