| utilization of substrate analogs by mengovirus induced rna polymerase. | | 1969 | 4305637 |
| [induction of interferon synthesis by double-stranded rna. i. application to the study of the multiplication cycle of mengo virus]. | | 1969 | 4307590 |
| influence of statolon on resistance of mice to influenza. | various interferon inducers are known to elicit protection against lethal or infecting doses of certain viral agents. because of the relatively high morbidity rate of influenza and its seasonal occurrence, we wished to determine whether statolon-induced interferon might be effective in controlling this disease. mice were treated intraperitoneally with statolon and challenged with influenza a(2) virus by the intranasal route. although interferon was present in the serum at the time of virus admin ... | 1969 | 4308934 |
| mengovirus-induced cytopathic effect in l-cells: protective effect of interferon. | the effect of interferon on mengovirus-induced cytopathic effect (cpe) in l cells, the cut-off of host-cell protein synthesis, and production of mature virus were found to be dependent on the concentration of interferon. cpe and inhibition of host protein synthesis were not affected until the concentration of interferon was increased 100-fold over that required to reduce viral yields by 90%. | 1969 | 4309883 |
| competitive inhibition of uridine incorporation by 6-azauridine in uninfected and mengovirus-infected novikoff hepatoma cells. | | 1969 | 4311690 |
| host-dependent restriction of mengovirus replication. | mengovirus infection of a restrictive cell line, maden's bovine kidney (mdbk), results in a virus yield 1,000-fold less than that obtained from productively infected cell lines such as l cells or ehrlich ascites tumor cells (eat). cells of both types of host systems are infected with comparable efficiencies and are completely killed as a consequence of infection. infective center assays, coupled with the observation of total cell killing, suggest that comparable numbers of cells synthesize viral ... | 1969 | 4311796 |
| insensitivity of mitochondrial rna synthesis to mengovirus infection in cho cells. | | 1969 | 4311805 |
| the step in cellular dna synthesis blocked by newcastle disease or mengovirus infection. | | 1970 | 4312372 |
| [interferon-inducing capacity of double-stranded rna. ii. studies on mengo virus replicatative intermediate]. | | 1970 | 4312964 |
| [fluorescence serology studies on the effects of antiviral agents on virus replication. i. the action of beta-2-dl-thienyl alanine on the replication of mengo virus]. | | 1969 | 4313030 |
| effect of prehumidification on sampling of selected airborne viruses. | studies were undertaken to determine if a prewetting device (humidifier bulb) used in combination with an all glass impinger (agi-30) would increase the recovery of airborne mengovirus-37a, vesicular stomatitis virus (vsv), and the s-13 coliphage. suspensions of t3 coliphage with mengovirus-37a, vsv, or s-13 were aerosolized and collected by using the agi-30-humidifier bulb combination to sample the aerosols before and after shifts in relative humidities (rh). these studies revealed the followin ... | 1969 | 4313171 |
| the structural proteins of mengo virus variants. | | 1970 | 4314506 |
| polyacetal carboxylic acids: a new group of antiviral polyanions. | chlorite-oxidized oxypolysaccharides are polyacetal carboxylic acids. they inhibited the cytopathic effect of vesicular stomatitis virus in mouse embryo cell cultures challenged at low input multiplicity. after intraperitoneal injection of these compounds in mice, interferon appeared in the circulation. the compounds also protected mice against lethal mengovirus infection and against the development of experimental pox lesions on the tail. chlorite-oxidized oxyamylose was antiviral only when at ... | 1970 | 4314553 |
| antiviral activity of chlorite-oxidized oxyamylose, a polyacetal carboxylic acid. | intraperitoneal injection of chlorite-oxidized oxyamylose (coam) protected mice against mengo, vaccinia, semliki forest, and influenza apr8 viruses. topical administration in the eye of rabbits partially inhibited the development of experimental herpetic keratoconjunctivitis. coam resembled polyacrylic acid in many aspects, but it was markedly less toxic. for systemic administration, the therapeutic index was on the order of magnitude of 1:300 to 1:500. although the in vivo antiviral effect of c ... | 1970 | 4314554 |
| physical and chemical studies of mengo virus variants. 3. absorbance--temperature profiles, sedimentation in dextran sulfate gradients, and total-infectious particle ratios. | | 1970 | 4315866 |
| inhibition of synchronized cellular deoxyribonucleic acid synthesis during newcastle disease virus, mengovirus, or reovirus infection. | cultures of l cells were synchronized with respect to deoxyribonucleic acid (dna) synthesis with thymidine and 5-fluoro-2'-deoxyuridine (fudr) and infected with newcastle disease virus (ndv), mengovirus, or reovirus 3. inhibition of incorporation of (3)h-cytidine into the dna of synchronized cells is partially inhibited 2 hr after infection with ndv or mengovirus and nearly completely suppressed 4 hr after infection. with ndv and mengovirus, no evidence was obtained of differences in sensitivity ... | 1970 | 4317111 |
| effect of statolon on aerogenic immunization of mice with mengovirus-37a. | | 1970 | 4317263 |
| circular dichroism studies on mengo virus variants and their constituent ribonucleates. | | 1970 | 4318119 |
| studies of the protein subunit of ph-inactivated mengo virus variants. i. polypeptide composition. | | 1970 | 4318981 |
| mengovirus rna synthesis in productive and restrictive cell lines. | | 1970 | 4318986 |
| influence of blood clearance rates on interferon production and virulence of mengo virus plaque mutants in mice. | | 1970 | 4319089 |
| comparison of agar and agarose preparations for mengovirus plaque formation. | an agarose overlay yielded mengovirus plaques earlier and in greater size and number than overlays of chemically undefined agars with or without enhancers. marked variability in plaque-forming efficacy of commercial agarose preparations was noted. | 1970 | 4320349 |
| growth of mouse l cells in shaken culture and mengovirus plaque formation on l cells suspended in agar. | procedures are described that require a minimum of equipment and maintenance for growing mouse l cells suspended in liquid medium and for plaquing mengovirus on l cells suspended in agar. viability of l cells during storage for 1 to 2 hr at relatively high concentrations was better in media at 30 c than at 0 c, as measured by viable counts after growth for 24 hr at 35 c. the number and size of plaques increased with increasing concentration of nahco(3) in the agar layers, but the relative differ ... | 1970 | 4320864 |
| abnormal dna patterns in animal mitochondria: ethidium bromide-induced breakdown of closed circular dna and conditions leading to oligomer accumulation. | treatment of cultured mouse fibroblasts (l-cells) and hamster kidney cells with ethidium bromide was found to inhibit the incorporation of [(3)h]thymidine into mitochondrial closed-circular dna, but not into nuclear dna. the specific activity of nuclear dna in treated cells was higher than in control cells. treatment also led to breakdown of closed-circular mitochondrial dna, greatly enlarged mitochondrial profiles with few or no cristae, and a higher yield of mitochondrial protein per cell.a si ... | 1970 | 4321352 |
| effect of mengovirus replication on choline metabolism and membrane formation in novikoff hepatoma cells. | infection of novikoff rat hepatoma cells (subline nlsl-67) with mengovirus resulted in a two- to threefold increase in the rate of choline incorporation into membrane phosphatidylcholine at about 3 hr after infection, without affecting the rate of transport of choline into the cell or its phosphorylation. the time course of virus-stimulated phosphatidylcholine synthesis was compared with the time courses of other virus-induced processes during a single cycle of replication. the formation of vira ... | 1970 | 4322083 |
| [electron microscopic detection of mengovirus in l cells with ferritin-labeled antibodies]. | | 1970 | 4322839 |
| immunofluorescent studies on the effect of antiviral substances on virus multiplication. ii. determination of fluorescence intensity in mengo virus infected l cells treated with beta-2-dl-thienylalanine. | | 1971 | 4325975 |
| immunofluorescent studies on the effect of antiviral substances on virus multiplication. 3. effect of actidione on the multiplication of mengo virus. | | 1971 | 4325976 |
| [demonstration of the mengo virus antigen in the mouse brain by help of the immunofluorescence technique]. | | 1971 | 4327126 |
| [antiviral activity of imidazole derivatives. i. inhibition of multiplication of mengovirus in fl cells]. | | 1971 | 4329638 |
| synthesis of ribonucleic acid by mengovirus-induced rna polymerase in vitro: nature of products and of rnase-resistant intermediate. | | 1968 | 4329736 |
| [antiviral activity of imidazole derivates. 3. autoradiographic studies on the influence of 1-butyl-4(4'-allyloxyphenyl)-imidazole-hydrochloride on rna synthesis in mengovirus infected fl cells]. | | 1971 | 4330137 |
| system of double infection between vaccinia virus and mengovirus. | when l cells are simultaneously infected with vaccinia virus and mengovirus, double interference in the replication of both viruses is observed. superinfection of vaccinia virus-infected cells by mengovirus during the first 5 hr of infection reduces vaccinia virus yields to between 1 and 3% of controls. the yields of mengovirus are reduced to between 1 and 16% of controls, depending upon the time of superinfection. the replication of vaccinia deoxyribonucleic acid is not inhibited by mengovirus; ... | 1971 | 4330362 |
| studies of the protein subunit of ph-inactivated mengo virus variants. ii. physicochemical properties. | | 1971 | 4330368 |
| nucleotide pools in novikoff rat hepatoma cells growing in suspension culture. 3. effects of nucleosides in medium on levels of nucleotides in separate nucleotide pools for nuclear and cytoplasmic rna synthesis. | this study was undertaken to measure the absolute levels of nucleoside pools in novikoff rat hepatoma cells (subline n1s1-67) during growth in suspension culture in the presence of high concentrations of various nucleosides in the medium, and to obtain further evidence for the compartmentalization of the nucleotides in independent cytoplasmic and nuclear pools. the levels of nucleotide pools were measured by growing the cells in medium supplemented with inorganic phosphate-(32)p. the nucleotide ... | 1972 | 4331295 |
| repair replication of hela cell deoxyribonucleic acid in cells infected with newcastle disease virus or mengovirus or treated with puromycin. | we examined repair replication of hela cell deoxyribonucleic acid (dna) in cells infected with mengovirus or newcastle disease virus or treated with puromycin. cellular dna was damaged by ultraviolet light and then pulse-labeled with (3)h-thymidine. autoradiographic analysis of non-s-phase dna synthesis (repair replication) showed that there was no inhibition of this process at a time when overall cellular dna synthesis was severely inhibited by either virus infection or puromycin treatment. | 1971 | 4331658 |
| a comparison of the protective effect against mengo virus infection in mice of interferons induced by polyi:c and mengo virus. | | 1971 | 4333910 |
| in vitro translation of cardiovirus ribonucleic acid by mammalian cell-free extracts. | cell-free extracts prepared from ehrlich ascites and mouse l cells synthesize viral proteins in response to encephalomyocarditis virus, mouse elberfeld virus, and mengovirus ribonucleic acid. although hela cell extracts are inactive, their ribosomes are functional in the presence of heterologous supernatant fractions. synthesis depends upon the addition of adenosine triphosphate, guanosine triphosphate, an energy-generating system, and 4 mm mg(2+). initiation is completed during the first 10 to ... | 1972 | 4336562 |
| differential inhibition of nuclear rna polymerases in l cells infected with mengovirus. | | 1972 | 4338796 |
| rna polymerase complexes from mengovirus infected cells. | | 1972 | 4341300 |
| capsid polypeptides of mouse elberfeld virus. i. amino acid compositions and molar ratios in the virion. | the four major polypeptide chains (alpha, beta, gamma, delta) constituting the capsid protein of mouse elberfeld (me) virus were isolated by preparative electrophoresis on polyacrylamide gels, and the amino acid composition of each chain was determined. in addition, the molecular weights of the smallest chains of me virus, mengovirus, and poliovirus, which had previously been determined by gel electrophoretic methods, were redetermined by gel filtration chromatography in 6 m guanidine hydrochlor ... | 1972 | 4342047 |
| molcular species of interferon induced in mice by poly i:c and mengo virus. | | 1972 | 4343060 |
| response of cultured mammalian cells to diphtheria toxin. v. concurrent resistance to ribonucleic acid viruses in diphtheria toxin-resistant kb cell strains. | it was determined that kb-r cell strains, isolated from the kb cell line and resistant to diphtheria toxin, also resist infection by poliovirus, mengo virus, vesicular stomatitis virus, and newcastle disease virus. this resistance manifests itself by reduction in yields of progeny virus (a reduction of more than 2 logs in some cases), reduced production of viral-specific ribonucleic acid (rna), and delayed cytopathic effect. in three kb-r strains tested, resistance was related to a step which fa ... | 1972 | 4344025 |
| mechanism of mengo virus-induced cell injury in l cells: use of inhibitors of protein synthesis to dissociate virus-specific events. | l cells were infected with mengo virus in the presence of varying concentrations of protein synthesis inhibitors (azetidine-2-carboxylic acid, p-fluorophenylalanine, puromycin), and examined with respect to the effects of the inhibitors on several features of virus-induced cell injury. the virus-specific events in the cells could be dissociated into three groups, based on their sensitivity to the inhibitors: (i) viral ribonucleic acid (rna) synthesis, bulk viral protein synthesis, and infectious ... | 1972 | 4344257 |
| repair replication: a density-gradient analysis of dna from cells infected with mengovirus or newcastle disease virus. | | 1972 | 4344315 |
| initiation of picornavirus protein synthesis in ascites cell extracts. | the current model of picornavirus protein formation implies that initiation of protein synthesis occurs at a single site on the viral rna, and that the large polypeptide formed is later cleaved. a direct test of this model was made in vitro by studying the incorporation of [(35)s]methionine from rabbit liver met-trna(m) (met) and fmet-trna(f) (met) into encephalomyocarditis virus rna-coded proteins in extracts of ehrlich ascites cells. the incorporation of n-formylmethionine was complete within ... | 1972 | 4345505 |
| purification of mengovirus and identification of an a-rich segment in its ribonucleic acid. | | 1972 | 4346654 |
| effect of phleomycin on the replication of mengovirus in novikoff hepatoma cells. | | 1973 | 4349394 |
| stability and function of mengovirus rna in cell-free protein synthesis. | | 1973 | 4351139 |
| discrimination between messenger ribonucleic acids by a mammalian translation initiation factor. | a factor from rabbit-reticulocyte ribosome, which stimulates by 5-fold initiation of hemoglobin mrna translation in krebs ascites cell extracts, was purified to homogeneity. the factor, a protein of about 65,000 molecular weight in sodium dodecyl sulfate, discriminates between various mrnas: it stimulates translation of alpha globin mrna and of tobacco mosaic virus rna, but has only a small effect on beta globin mrna and no effect at all on mengo virus rna translation. | 1973 | 4352974 |
| correlation between the antiviral effect of interferon treatment and the inhibition of in vitro mrna translation in noninfected l cells. | when noninfected l-cell suspension cultures are treated with interferon (specific activities superior to 10(6) reference units per mg of protein), the cell-free cytoplasmic extracts obtained are inactive for the translation of exogenous natural mrnas. the dose-response curve shows that comparable amounts of interferon are required to produce a 50% reduction of mengo virus multiplication in vivo and mengo rna translation in vitro. with higher doses of interferon, mengo rna translation is complete ... | 1973 | 4355848 |
| in vitro translation of oogenetic messenger rna of sea urchin eggs and picornavirus rna with a cell-free system from sarcoma 180. | a cell-free protein-synthesizing system prepared from mouse sarcoma 180 was characterized by use of rna from mengo virus and sea urchin egg. in the presence of exogenous mammalian transfer rna, total sea urchin egg rna and mengo rna direct incorporation of [(3)h]leucine into acid-insoluble material. the system is extremely efficient in that a stimulation of 100-times over background can be obtained. studies with formylmethionyl-transfer rna, as well as with inhibitors of initiation, indicate tha ... | 1973 | 4357864 |
| alteration in trna methyltransferase activity in mengovirus infection: host range specificity. | the trna methyltransferase activity in mengovirus-infected l cells, hela cells, and maden derby bovine kidney cells has been examined during the course of infection. the first two cell lines yield a productive infection, but have different kinetics of inhibition of host rna synthesis, whereas the bovine kidney cells are a restrictive host. in infected l cells the enzymes show altered capacity and base specificity throughout the infection. in infected hela cells and in infected bovine kidney cell ... | 1973 | 4358158 |
| fate of mrna of l-cells infected with mengovirus. | mengovirus infection of l-cells results in an inhibition of host protein synthesis which is detectable in vivo by a decreased rate of incorporation of radioactive amino acids into acid-insoluble material and by a concomitant reduction in polysome content. the inhibition of host protein synthesis occurs early in the infection cycle, at a time when there is little synthesis of viral proteins. in this paper the stability of polyadenylic acid [poly(a)]-containing mrna of uninfected l-cells and cells ... | 1974 | 4362513 |
| "in vitro" binding of cellular, alpha-amanitin sensitive, rna polymerase to infectious, mengovirus-induced double-stranded rna. | | 1974 | 4363641 |
| formation of mengovirus-like particles in cell-free extracts from virus-infected cells. | | 1974 | 4363940 |
| double-stranded ribonucleic acid from mengo virus: production, characterization, and interferon-inducing and antiviral activities in comparison with polyriboinosinic-polyribocytidylic acid. | mengo virus double-stranded ribonucleic acid (dsrna) was obtained on a semi-industrial scale from infected cultures of bhk-21 cells grown in suspension. yield of the extraction and purification operations was small (about 22 mg from 10(11) cells in a 100-liter culture). physicochemical characterization of this dsrna gave an estimated molecular weight close to 4 x 10(6), a density of 1.59 (similar to that of the poliovirus dsrna), and a thermal transition midpoint of 94 c. this product was a litt ... | 1973 | 4364180 |
| enzymatic acylation of histidine to mengovirus rna. | | 1974 | 4364352 |
| translation of mengovirus rna in ehrlich ascites cell extracts. | | 1974 | 4364564 |
| responsiveness of lymphocytes from mengovirus treated mice to phytohaemagglutinin stimulation, in vitro. | | 1974 | 4365618 |
| cleavage of mengovirus polyproteins in vivo. | the synthesis of mengovirus-specific proteins in vivo was studied by labeling the viral proteins with radioactive amino acids under conditions in which host protein synthesis was almost completely inhibited. pulse-chase experiments enabled the kinetic analysis of the cleavages of certain viral protein precursors and the formation of others. the pattern of cleavages of mengovirus precursor polypeptides is similar to that of encephalomyocarditis virus. the major difference between the two viruses ... | 1974 | 4367902 |
| testing of antiviral compounds against mengo virus infection of mice. i. screening of compounds and statistical evaluation of their effects. | | 1974 | 4370429 |
| recovery of dna-dependent rna polymerase activities from l cells after mengovirus infection. | | 1974 | 4371761 |
| infectivity of mengovirus replicative form. relationship to cellular transcription. | | 1974 | 4372317 |
| antiviral 1,3,4-thiadiazoles. i. action on multiplication of mengo virus in fl cells. | | 1974 | 4374341 |
| polypeptides associated with the 250 s mengovirus-induced rna polymerase structure. | | 1974 | 4375446 |
| iron-binding catechols and virulence in escherichia coli. | a comparison of viral-induced unresponsiveness of phytohemagglutinin (pha)-induced deoxyribonucleic acid (dna) synthesis of mouse lymphocytes was made by culturing the cells under identical conditions in the presence of hepes buffer in a humid-air atmosphere. the degree of pha-induced dna synthesis was found to vary, depending upon the type of ribonucleic acid or dna virus treatment. myxovirus, paramyxoviruses, mengo virus, leukemic viruses, herpesvirus, and vaccinia virus caused a depression in ... | 1973 | 4576680 |
| the interaction of mengo virus ribonucleic acid with l, hela and human diploid cells: a comparative study. | | 1970 | 5438061 |
| transport as the rate-limiting step in the incorporation of uridine into mengovirus ribonucleic acid in novikoff rat hepatoma cells. | the incorporation of uridine into the nucleotide pool of actinomycin-treated, mengovirus-infected novikoff rat hepatoma cells in culture follows simple michaelis-menten kinetics, and the apparent v(max) and k(m) values are similar to those for uridine transport by uninfected cells. incorporation of uridine into mengovirus-specific ribonucleic acid (rna) also follows michaelis-menten kinetics, and the apparent k(m) (about 10 mum) is approximately the same as for uridine transport. inhibition of u ... | 1971 | 5543426 |
| effects of elevated temperatures on mengovirus ribonucleic acid synthesis and virus production in novikoff rat hepatoma cells. | the production of mengovirus in novikoff rat hepatoma cells is progressively reduced with an increase in incubation temperature of the cells from 34 to 40 c, in spite of the fact that about the same amounts of single-stranded and double-stranded viral ribonucleic acid (rna) are synthesized at 34, 37, and 40 c; the rate of overall protein synthesis is as high at 40 c as at 37 c. at 40 c, progeny viral rna accumulates in an undegraded form without being incorporated into virus particles. the resul ... | 1971 | 5543427 |
| delayed death associated with statolon treatment of mice infected with mengovirus. | | 1968 | 5718719 |
| ribosomes: effect of interferon on their interaction with rapidly labeled cellular and viral rna's. | rapidly labeled rna of mouse l cells and labeled rna of mengo virus, unlike cellular rna labeled under steady-state conditions, form detectable complexes with l-cell ribosomes. these ribosome-rna complexes formed in vitro appear analogous to those assembled during polysome formation in vivo. when ribosomes are prepared from l cells exposed to homologous interferon, their capacity to associate with cell messenger is preserved, while their ability to interact with viral rna is markedly reduced. th ... | 1967 | 6018644 |
| functional characterization of eukaryotic mrna cap binding protein complex: effects on translation of capped and naturally uncapped rnas. | we examined the effects of a eukaryotic mrna cap binding protein (cbp) complex purified by cap analogue affinity chromatography [edery, i., humebelin, m., darveau, a., lee, k.a. w., milburn, s., hershey, j.w.b., trachsel, h., & sonenberg, n. (1983) j. biol. chem. 258, 11398 11403], on translation of several capped and naturally uncapped mrnas in extracts prepared from poliovirus-infected or mock-infected hela cells. the cbp complex has activity that restores capped mrna (globin, tobacco mosaic v ... | 1984 | 6089873 |
| structure of the mengo virion. vii. crystallization and preliminary x-ray diffraction analysis. | crystals of mengo virions have been grown reproducibly and analyzed by x-ray diffraction. these crystals diffract to a resolution of 7.0 a. the unit cell exhibits cubic symmetry with a = 422 a. the space group is p23, with four virus particles situated on crystallographic threefold axes. picornavirions from three of the four recognized genera (study group on picornaviridae, intervirology 10, 165-180, 1978) have now been examined at low resolution by x-ray diffraction: poliovirus type 1 (j. t. fi ... | 1984 | 6093358 |
| potentiation of interferon's antiviral activity by the mutually synergistic interaction of muifn-alpha/beta and muifn-gamma. | the interaction of the interferons (ifns) that cooperate to potentiate the antiviral action of ifn was studied. serial dilutions of muifn-gamma and muifn-alpha/beta were employed separately and in combination to block virus replication in one-step, virus yield reduction experiments. to calculate the potentiation of ifn activity, protection levels obtained for each combination of muifn-gamma and muifn-alpha/beta were compared with those obtained for the separate ifns. potentiation levels increase ... | 1984 | 6096507 |
| picornaviruses of two different genera have similar structures. | crystals of mengo virus were used to collect three-dimensional x-ray diffraction data to 7 a resolution. a self-rotation function showed the precise orientation of the mengo particles in the crystal unit cell. a cross-rotation function against similar data of cubic rhinovirus crystals showed a peak when the orientations of these two icosahedral viruses were superimposed. this demonstrates similarity of capsid construction between two picornaviruses of different taxonomic genera. | 1984 | 6098687 |
| effect of n, n'-bis(methylisatin-beta-thiosemicarbazone)-2-methylpiperazine against virus-induced encephalitis in mice). | n,n'-bis(methylisatin-beta-thiosemicarbazone)-2-methylpiperazine (tski-vi) proved to be significantly effective against lethal vaccinia, pseudorabies and mengo virus-induced encephalitis in different strains of mice when administered subcutaneously (s.c.) in doses of 20 mg/kg body weight, twice daily, for a period of five days. the strongest effects occurred in vaccinia virus-infected mice, and the degree of protection was both dose- and virus-dependent. titres of vaccinia virus in brains of inf ... | 1980 | 6111204 |
| antiviral activity of hetarylhydrazones. | some hetarylhydrazones showed antiviral activity against mengo virus in vitro. the replication of influenza a and b viruses was affected neither in vitro (chick chorioallantoic membranes) nor in vivo (mice). based on dose response curves of the active hetarylhydrazones the compound z 98/69 appeared most effective in vitro. this compound neither inactivated the extracellular virus nor inhibited its adsorption and penetration; it reduced virus replication when added 1-3 hr after mengo virus inocul ... | 1982 | 6127013 |
| effect of quercetin on the course of mengo virus infection in immunodeficient and normal mice. a histologic study. | quercetin protects mice from lethal mengo m virus infection when given orally 12 and 1 hr before and 8, 24, 36, 48 and 56 hr after inoculation. no differences in the course of infection have been found between normal splenectomized or congenitally athymic mice. likewise the effect of drug treatment was similar in all three models. necrotic lesions in the main target organs (central nervous system, salivary and lacrimal glands, thymus, pancreas, kidneys and spleen) from both normal and immunodefi ... | 1982 | 6127014 |
| interferon in experimental viral infections in mice: tissue interferon levels resulting from the virus infection and from exogenous interferon therapy. | in mice given single intraperitoneal doses of interferon, serum interferon levels peaked at 1 h postinjection and were reduced to zero at about 8 h. the interferon concentrations in spleen, liver, and lungs were about 100-fold higher than could be expected from the amount of serum contained in these organs. in the brain only low levels of antiviral activity were detected. in mice infected intraperitoneally with mengo virus, viral replication in the brain occurred around day 4 and was accompanied ... | 1980 | 6160109 |
| recovery by interferon of mengovirus-induced shutoff of host protein synthesis. | | 1980 | 6165301 |
| mechanism of action of antiviral hetarylhydrazones in vitro. | three antiviral hetarylhydrazones showed a depression of 3h-uridine incorporation into rna of fl cells as a consequence of inhibition of uridine transport into the cells. by eliminating the action of the compounds on transport processes with the prelabelling method, only one compound showed a nearly 50% reduction of cellular rna synthesis during the whole incubation period, whereas the other two compounds had no influence during the first 6 hr. under prelabelling conditions all three compounds c ... | 1981 | 6166184 |
| attachment of avidin-coupled spheres to linear and circular forms of mengovirus double-stranded rna. | picornavirus-infected cells contain a double-stranded rna (replicative form [rf] rna), composed of viral genomic rna hydrogen bonded to crna of similar nucleotide length. mengovirus rf rna reacted with a succinimide ester of biotin was shown by electron microscopy to bind avidin-coupled polymethacrylate spheres. these binding sites are taken to indicate the presence of vpg protein molecular by methods previously applied to poliovirus rf rna (richards et al., proc. natl. acad. sci. u.s.a. 76:676- ... | 1981 | 6168770 |
| studies on an interferon-sensitive mutant of mengovirus: effects on host rna and protein syntheses. | interferon induces an activity which strongly inhibits the growth of is-1, an interferon-response mutant of mengovirus. this activity is not expressed in protected cells infected with either is+ (the wild-type parent) or vaccinia virus, or in cells infected with is-1 in the presence of actinomycin d. a failure of is-1 to shut off host rna and/or protein synthesis could explain these observations. the present paper, however, shows that is-1 and is+ are equally effective in suppressing host synthe ... | 1981 | 6169788 |
| novel circular forms of mengovirus-specific double-stranded rna detected by electron microscopy. | | 1982 | 6175078 |
| effect of interferon on transient shut-off of cellular rna and protein synthesis induced by mengo virus infection. | infection of mouse l929 cells with mengo virus resulted in a rapid shut-off of cellular rna synthesis followed within the first hours post infection by a gradual decrease in host protein synthesis. pretreatment of the cells with high doses of interferon, blocking viral multiplication, did not affect the virus-induced shut-off of host macromolecular synthesis. in these interferon-treated cells the 2',5'a-activated nuclease may account for the degradation of viral rna, soon after its replication. ... | 1981 | 6175273 |
| mechanism of antiviral action of quercetin against cardiovirus infection in mice. | oral treatment with quercetin protected abd2f1/jena mice significantly against intraperitoneal encephalomyocarditis, col, sk, mm, mengo m,l and mengom virus infections, but not against intracerebral challenge with mengom virus. enhanced resistance to mengom virus were induced in the genetically different dba 2/jena, c57bl/jena, c57bl/lati and abc2f1/jena mice, c57bl6/jena nu/nu mice were also protected, indicating that the thymus was non-essential to the protective effects of quercetin. in ab/je ... | 1981 | 6176184 |
| interferon induction by viruses. vii. mengovirus: "interferon-sensitive" mutant phenotype attributed to interferon-inducing particle activity. | | 1981 | 6180093 |
| protein synthesis in extracts from interferon-treated mengo virus infected cells. | | 1982 | 6182881 |
| genomic rna of mengovirus. vi. translation of its two cistrons in lysates of interferon-treated cells. | the addition of low levels (40 ng/ml) of the synthetic double-stranded polyribonucleotide poly i:c to lysates of interferon-treated l-cells resulted in a strong inhibition (70 to 75%) of the in vitro translation of mengovirus rna. under these conditions, the rates of incorporation of [35s]methionine or formyl-[35s]methionine were depressed to a comparable extent. the sequences of mengovirus rna recognized by ribosomes of interferon-treated cells at initiation of translation were compared with th ... | 1983 | 6187938 |
| antiviral activities directed against wild-type and interferon-sensitive mengovirus. | two distinct antiviral activities can be detected in l cells treated with low levels of interferon and infected with a one-step interferon-sensitive mutant of mengovirus (is-1). the first antiviral activity (ava-1) primarily delayed virus rna and protein synthesis and thereby lengthened the virus replication cycle. it did not prevent cell death. the second antiviral activity (ava-2) allowed the virus-induced inhibition of host macromolecular synthesis but inhibited all other virus functions. by ... | 1983 | 6190989 |
| the role of interferon induction in the interferon sensitivity of the mengovirus mutant, is-1. | an interferon-sensitive mutant of mengovirus has been shown to specifically induce interferon in infected cells. although this appears to account for the sensitivity to interferon observed by others in an l(y) cell line, it cannot account for the even greater sensitivity observed in our g3 line of mouse l cells. | 1984 | 6198449 |
| antiviral activity of a thymic factor in experimental viral infections. i. thymic hormonal effect on survival, interferon production and nk cell activity in mengo virus-infected mice. | a partially purified thymic factor, thymostimulin (ts), significantly increased the survival rate of adult, immune-intact mice infected with the neurotropic mengo virus. ts treatment was begun after virus inoculation by daily i.p. injections. in untreated c57bl/6 mice, ld50 was reached with 1 x 10(4) pfu, but 10-fold more virus (i.e., 1 x 10(5) pfu) was needed to reach ld50 in ts-treated animals. ts effect on survival, though, could be observed with several virus doses (1 x 10(3) to 1 x 10(6) pf ... | 1984 | 6202776 |
| transformation by murine sarcoma virus alters the sensitivity of clonal cells derived from nih/3t3 mouse fibroblasts to interferon. | in contrast to normal clonal cells (a5) derived from nih/3t3 mouse fibroblasts, another clone (a10) derived from the same source was found to be resistant to the anti-lytic-virus activity of ifn and to be deficient in the induction of (2'-5') oligoadenylate synthetase (2-5a synthetase) by ifn. following infection of either a5 or a10 cells with moloney murine sarcoma virus (msv), a few transformed colonies were isolated, expanded, and tested for their sensitivity to ifn. it is clearly demonstrate ... | 1984 | 6206644 |
| fragility of the rhinovirus type 14 genome to incubation at 60 degrees. | rhinovirus type 14 (rv14) genomic rna, but not mengovirus or coxsackievirus b3 genomic rna, fragmented to acid-insoluble rna species during incubation in aqueous buffers at 60 degrees. incubation of rv14 genomic rna in high concentrations of dimethyl sulfoxide at 60 degrees did not result in fragmentation, suggesting that fragmentation was not a result of pre-existing nicks. rv14rna that was treated with proteinase k prior to incubation at 60 degrees did not undergo fragmentation. the associatio ... | 1980 | 6244243 |
| inhibition of mengovirus rna-dependent rna polymerase by an isatinisothiosemicarbazone and a piperidine-thiocarbonyl-hydrazone derivative in a cell-free system. | the inhibitory action of two antiviral compounds used at the maximum tolerated dosis in a cellular system led to a complete suppression of the infectious mengovirus yield and to a 100% plaque reduction. the products of the rna polymerase reaction catalyzed by the microsomal-mitochondrial fraction of mengovirus-infected fl cells were analyzed by linear sucrose gradient centrifugation and polyacrylamid gel electrophoresis. the results showed that the inhibitors cause a general reduction of the syn ... | 1980 | 6250278 |
| the influence of the host cell on the inhibition of virus protein synthesis in cells double infected with vesicular stomatitis virus and mengovirus. | the ability of mengovirus to inhibit the synthesis of vesicular stomatitis virus (vsv) proteins and of vsv to inhibit the synthesis of mengovirus proteins during double infection in three different cell lines was investigated. although cellular protein synthesis was inhibited after infection of cells by each virus, the ability of one virus to decrease translation of the mrna species of the co-infecting virus varied with the cell type. superinfection of mengovirus-infected l-929 cells by vsv resu ... | 1980 | 6257824 |
| replacement of mouse lm fibroblast choline by a sulfonium analog. effects on membrane properties as determined by virus probes. | a sulfonium analog of choline ('sulfocholine', a natural phospholipid constituent of diatoms) was metabolically incorporated into mouse lm fibroblasts cultured in serum-free medium. subconfluent cultures of lm cells were able to utilize sulfocholine as sole choline source and to increase in cell number for 3 days of incubation; thereafter a decrease in cell number was observed. in contrast, cultures of lm cells seeded to confluency showed no decrease in cell number up to at least 10 days when ma ... | 1981 | 6260173 |
| [on the biological action of transition metal complexes. 1. the antiviral activity of palladium aminopyridin-complexes (author's transl)]. | some aminopyridine complexes of palladium and pdcl2 showed an antiviral in vitro activity against enveloped dna and rna viruses such as vaccinia virus, pseudorabies virus, ndv and fpv. in contrast, naked rna virus as mengovirus was not affected. the compounds were compatible for chicken embryo as well as fl cells in concentrations of 100-250 microm. the therapeutical index calculated from the maximally tolerated dose and the concentration causing a 50 per cent plaque reduction was determined wit ... | 1981 | 6267845 |
| translational control by messenger rna competition for eukaryotic initiation factor 2. | translation of globin mrna in a micrococcal nuclease-treated reticulocyte lysate was studied in the presence of increasing amounts of mengovirus rna, under conditions in which the number of translation initiation events remains constant as judged by the transfer of label from n-formyl[35s]methionyl-trnaf into protein. the translation of globin mrna is progressively inhibited by low concentrations of mengovirus rna, free of detectable traces of double-stranded rna, concomitant with the increasing ... | 1982 | 6274873 |