| chromosomal sublocalization of human c-myb and c-fes cellular onc genes. | the transforming genes of acutely transforming retroviruses are derived from conserved cellular genes (c-onc genes) which are believed to be important in normal cell growth and differentiation. recent studies indicate that altered expression of c-onc genes, for example, by insertion of viral genomes, gene amplification or chromosomal translocation, can lead to development of malignant diseases in man and animals. c-myb and c-fes are homologues of the transforming genes of avian myeloblastosis vi ... | 1983 | 6866112 |
| experimental anterior uveitis after subcutaneous injection of feline sarcoma virus. | feline sarcoma virus (fesv) is a naturally occurring virus that causes spontaneous tumors in cats. the immunologic and morphologic characteristics of these tumors have been studied extensively. it was recently observed in experiments undertaken to induce systemic malignancy with this virus, that severe uveitis and clinical blindness occurred. an investigation of the ophthalmologic changes was undertaken. a fulminent anterior uveitis was produced in cats by a series of subcutaneous injections of ... | 1983 | 6874270 |
| retrovirus-encoded transformation-specific polyproteins: expression coordinated with malignant phenotype in cells from different germ layers. | a transformation-associated polyprotein designated "gag-x" was previously shown to be induced by the feline sarcoma virus (fesv) after the nonproductive transformation of rat or mink cells. we found that this protein was also expressed in cells derived from the native species (cat) with or without the production of feline leukemia helper virus (felv) and that cats could mount a humoral antibody response to the transformation-specific (x) portion of the molecule. such antisera also reacted with t ... | 1981 | 6973761 |
| feline sarcoma virus p115-associated protein kinase phosphorylates tyrosine. identification of a cellular substrate conserved during evolution. | the gardner strain of feline sarcoma virus (fesv) has previously been shown to encode as its major translational product, a phosphoprotein of molecular weight (mr) = 115,000 (p115) with an associated protein kinase activity and probable transforming function. in the present study, we demonstrate that this enzyme specifically phosphorylates tyrosine and that gardner fesv-transformed mink and rat cells exhibit constitutively higher levels of phosphotyrosine than nontransformed control cells. in ad ... | 1980 | 7430166 |
| v-onc mutation associated with host cell growth in retroviral tumors. | in sarcomagenesis in rats infected neonatally with feline sarcoma virus (st-fesv), v-fes product (p85) was previously shown by us to be a predictive and preventive determinant. in order to explore the part played by p85 in tumor suppression, dna was extracted from precancerous granulomas and from slow or rapid growing sarcomas induced by neonatal injection of the virus. the v-fes signal from extracted dna was analyzed by pcr-sscp. the prototype v-fes gene signal was detected in most lesions and ... | 1995 | 7475323 |
| oncogenic activation of v-kit involves deletion of a putative tyrosine-substrate interaction site. | the transforming gene of the hardy-zuckerman-4 strain of feline sarcoma virus, v-kit, arose by transduction of the cellular c-kit gene, which encodes the receptor tyrosine kinase (rtk) p145c-kit. to gain insight into the molecular basis of the v-kit transforming potential, we characterized the feline c-kit by cdna cloning. comparison of the feline v-kit and c-kit sequences revealed, in addition to deletions of the extracellular and transmembrane domains, three additional mutations in the v-kit o ... | 1995 | 7530827 |
| malignant transformation of a human fibroblast cell strain by transfection of a v-fes oncogene but not by transfection of a gag-human c-fes construct. | to determine whether the human c-fes gene, a homologue of the feline v-fes oncogene, can play a role in the malignant transformation of human fibroblasts, we transfected a near-diploid, infinite life span, growth factor-independent, human fibroblast cell strain, msu-1.2, with plasmids carrying a fes gene along with a selectable marker. the fes gene was either the v-fes oncogene from the gardner-arnstein strain of feline sarcoma virus or a chimeric construct in which 835 base pairs representing e ... | 1994 | 7696187 |
| conversion of human fibroblasts to tissue macrophages by the snyder-theilen feline sarcoma virus (st:fesv(felv)): productive infection by leishmania major. | the decisive role of macrophages in t-cell differentiation is best exemplified by cutaneous leishmaniasis. during infection, leishmania attach to macrophages, the only site of replication for the parasite. we have recently demonstrated the conversion of human fibroblasts to tissue macrophages (tm) by transduction with the snyder-theilen feline sarcoma virus (st:fesv-(felv)). since leishmania have tropism only for macrophages, we have used the parasite to ascertain the functional phenotype of the ... | 1994 | 7721332 |
| structural alterations in the carboxyl-terminal domain of the bcrabl gene product activate its fibroblastic transforming potential. | activation of the c-abl protooncogene occurs in abelson murine leukemia virus, hardy-zuckerman-2 feline sarcoma virus, and during the chromosomal translocation that generates the bcrabl fusion gene. the three genes exhibit varying degrees of transforming activity; the two viral genes transform nih-3t3 cells in vitro, whereas the bcrabl gene is incapable of transforming these cells. to determine whether genetic alterations can enhance the transforming potential of the bcrabl gene, we employed gen ... | 1994 | 8106523 |
| suppression of retroviral tumors in hosts immune to viral oncogene product. | feline sarcoma virus of snyder-theilen strain (st-fesv) induces sarcomas in wistar/ma rats following neonatal virus injection. induced tumors express the viral oncogene product (p85) and elicit in hosts the specific serum anti-p85 antibody detectable by western blot analysis. syngeneic adult female rats were immunized with an st-fesv induced sarcoma that was 100% transplantable to syngeneic adult rats. newborns from immunized rats (vaccinated rats) were found to carry anti-p85 in their sera at b ... | 1994 | 8152290 |
| epidermal growth factor (egf) modulation of feline sarcoma virus fms tyrosine kinase activity, internalization, degradation, and transforming potential in an egf receptor/v-fms chimera. | the feline sarcoma virus oncogene v-fms has significantly contributed to the dissection of peptide growth factor action since it encodes the transmembrane tyrosine kinase gp140v-fms, a transforming version of colony-stimulating factor 1 receptor, a member of the growth factor receptor tyrosine kinase family. in this study, the functional significance of structural differences between distinct tyrosine kinase types, in particular between cellular receptors and viral transforming proteins of disti ... | 1994 | 8254751 |
| conversion of human fibroblasts to tissue macrophages by the snyder-theilen sarcoma virus (st:fesv): tumouricidal effects on k-562 and daudi tumour cell lines in monolayers and in agar suspensions. | we have previously demonstrated the conversion of human fibroblasts (hf) to tissue macrophages by transduction with the snyder-theilen feline sarcoma virus (st:fesv) [1-3]. the st:fesv-induced tm have been characterized both phenotypically and functionally, including their tumouricidal potential against colon adenocarcinoma (ls 180) cells. the present results show that st:fesv-induced tm produced significant lysis of k-562 tumour cells, but essentially no lysis of the daudi tumour cells. lysis o ... | 1993 | 8268416 |
| characterization of the transforming domain of a feline sarcoma virus encoding a fgr-related tyrosine kinase. | we investigated the transforming domain of a recently isolated feline sarcoma virus (tp1-fesv) which encodes a fgr-related tyrosine kinase expressed as a gag-fgr fusion protein. the gag portion was removed and replication-competent expression vectors (rcas) with inserted v-fgr sequences were established. chicken embryo fibroblasts (cef) were transfected and monitored for replication, integration and transcription of the proviral constructs. we demonstrated that transfected cells display morpholo ... | 1994 | 8279950 |
| use of immunohistochemistry and polymerase chain reaction for detection of oncornaviruses in formalin-fixed, paraffin-embedded fibrosarcomas from cats. | to determine whether there was intralesional infection or expression of felv or feline sarcoma virus in suspected vaccine-associated fibrosarcomas in cats. | 1996 | 8756876 |
| differentiation of human fibroblasts to tissue macrophages by the snyder-theilen feline sarcoma virus (st:fesv): growth modulation of human tumor cell lines in agar. | snyder-theilen feline sarcoma virus (st:fesv)-transduced human fibroblasts differentiate into tissue macrophages(1-9). the st:fesv-induced macrophages demonstrate macrophage-mediated cytotoxicity (mtc) and antibody-dependent cellular cytotoxicity (adcc), including growth modulation of tumor cells in agar (3,4,6). | 1997 | 9252687 |
| the role of v-fgr myristoylation and the gag domain in membrane binding and cellular transformation. | the v-fgr oncogene encodes a chimeric oncoprotein composed of feline sarcoma virus (fesv)-derived gag and cellular-derived actin and c-fgr sequences. v-fgr is myristoylated and membrane bound, two criteria which must be met for src kinases to induce cellular transformation. although inhibition of myristoylation resulted in a decreased ability of v-fgr to sediment with membranes from an nih-3t3 p100 fraction, deletion of the gag domain caused nearly all of the protein to remain unbound and cytoso ... | 1998 | 9740771 |
| the use of immunohistochemistry and the polymerase chain reaction for detection of feline leukemia virus and feline sarcoma virus in six cases of feline ocular sarcoma. | ocular sarcoma was diagnosed by light microscopic examination in enucleated globes (n = 4), orbital tissue biopsy (n = 1) and ocular evisceration contents (n = 1) from six cats. to determine if feline leukemia virus (felv) or a replication-defective felv, feline sarcoma virus (fesv), was present in these ocular sarcomas, immunohistochemistry (ihc) and polymerase chain reaction (pcr) for felv were utilized. immunohistochemical staining for felv glycoprotein 70 (gp70) was performed on all six form ... | 1998 | 11397230 |
| use of nested polymerase chain reaction (pcr) for detection of retroviruses from formalin-fixed, paraffin-embedded uveal melanomas in cats. | thirty-six formalin-fixed, paraffin-embedded enucleated globes from cats with a diagnosis of diffuse anterior uveal melanoma were obtained. sections of tumor were excised, deparaffinized, and subjected to nested polymerase chain reaction (pcr) to identify proviral dna sequences from the feline leukemia virus (felv)-feline sarcoma virus (fesv; 36 eyes), and the feline immunodeficiency virus (fiv; 18 eyes). all samples tested were negative for fiv dna. three samples were positive for felv-fesv dna ... | 1999 | 11397251 |
| c-cbl associates directly with the c-terminal tail of the receptor for the macrophage colony-stimulating factor, c-fms, and down-modulates this receptor but not the viral oncogene v-fms. | the receptor for the macrophage colony-stimulating factor (csf-1, also termed m-csf), the tyrosine kinase c-fms, was originally determined to be the oncogene product of the mcdonough strain of feline sarcoma virus, v-fms. the structural difference between c-fms and v-fms amounts to only five point mutations in the extracellular domain, two mutations in the cytoplasmic domain, and the replacement of 50 amino acids by 14 unrelated amino acids at the c-terminal tail. here, we have identified c-cbl ... | 2002 | 11847211 |
| lack of detection of feline leukemia and feline sarcoma viruses in diffuse iris melanomas of cats by immunohistochemistry and polymerase chain reaction. | diffuse iris melanoma was confirmed by light-microscopic examination in 10 formalin-fixed, paraffin-embedded globes from 10 cats. to determine if feline leukemia virus or a replication defective feline leukemia virus, feline sarcoma virus, was present in these anterior uveal melanomas, immunohistochemistry and polymerase chain reaction for feline leukemia virus were utilized. immunohistochemical staining for feline leukemia virus glycoprotein 70 was performed on all 10 tumors using an avidin-bio ... | 2002 | 12152818 |
| multicentric fibrosarcoma in a cat and a review of the literature. | a case of multicentric fibrosarcoma in a ten month old domestic short-haired cat is presented and discussed. tumor tissue was found to involve the right distal forepaw, right shoulder area and a popliteal lymph node. this anaplastic neoplasm was concentrated primarily in subcutaneous tissues but also extended to muscle, bone and lung. the cat was found to be positive for feline leukemia virus by the elisa test. based on these findings, it is likely that the lesions in this case result from an in ... | 1984 | 17422403 |
| feline vaccine-associated fibrosarcoma induced by aluminium compound in two cats: short communication. | two cases of feline vaccine-associated fibrosarcoma (fvaf) are reported. the excised tumours were both characterised as well circumscribed, subcutaneous, firm and white with central necrosis. histopathologically, they consisted of well-differentiated and variably sized and shaped anaplastic cells, characterised by marked nuclear and cellular pleomorphism including giant cells. the mitotic activity was low. aluminium was demonstrated in the central necrosis and giant cells. neoplastic cells were ... | 2008 | 18401961 |
| effects of the cfms kinase inhibitor 5-(3-methoxy-4-((4-methoxybenzyl)oxy)benzyl)pyrimidine-2,4-diamine (gw2580) in normal and arthritic rats. | the cfms (cellular homolog of the v-fms oncogene product of the susan mcdonough strain of feline sarcoma virus) (proc natl acad sci u s a 83:3331-3335, 1986) kinase inhibitor 5-(3-methoxy-4-((4-methoxybenzyl)oxy)benzyl)pyrimidine-2,4-diamine (gw2580) inhibits colony-stimulating factor (csf)-1-induced monocyte growth and bone degradation in vitro and inhibits csf-1 signaling through cfms kinase in 4-day models in mice (proc natl acad sci u s a 102:16078, 2005). in the present study, the kinase se ... | 2008 | 18434589 |
| prediction of kit mutation in gastrointestinal stromal tumors by the immunoprofile of the tumor cells. | human kit protooncogene is the cellular homolog of v-kit from the hardy-zuckerman 4 feline sarcoma virus, and encodes a 145-kda type iii tyrosine kinase growth factor receptor that is often mutated in gastrointestinal stromal tumors (gist). standardized mutation analysis is not available in many countries; therefore, we aimed to determine if the presence of kit mutation in gist can be predicted by the immunoprofile of the tumor cells. | 2010 | 20123583 |
| viral gene mutation as determinant of low grade malignancy of lesions induced by perinatal retrovirus infection. | of 108 wistar/ma rats perinatally injected with snyder-theilen feline sarcoma virus, 75 developed tumors within 60 days (group a), and 25 after more than 117 days (group b). metastases were detected in 59.1% and 11.8% in group a and b respectively. antibody to v-fes gene product was found to occur before tumor development in group b and after tumor development in group a. v-ses was found to be down-regulated and extensively mutated in tumors of group b. these results suggest viral gene mutation ... | 1997 | 21533410 |
| differentiation of human fibroblasts to tissue macrophages by the snyder-theilen feline sarcoma virus (st:fesv). | we have previously reported the conversion of human fibroblasts (hf) to tissue macrophages (tm) by transduction with the snyder-theilen feline sarcoma virus [st:fesv(felv)]. here, we have demonstrated macrophage mediated tumor cytotoxicity (mtc) of human teratocarcinoma cells (ntera-2) by st:fesv-induced tm. inclusion of the ntera-2 specific antibody (ssea-4), substantially increased antibody-dependent cellular cytotoxicity (adcc) of the tumor cells by st:fesv-induced tm. tumor cell lysis by st: ... | 1996 | 21544403 |
| differentiation of human fibroblasts to tissue macrophages by the snyder-theilen feline sarcoma virus is accompanied by nuclear accumulation of the tumor suppressor p53. | snyder-theilen feline sarcoma virus (st:fesv)-transduced human fibroblasts differentiate into tissue macrophages with many of the properties of normal macrophages. these cells express high levels of the gag-fes tyrosine kinase fusion protein, p85(v-fes), and exhibit an elevated level of tyrosine phosphorylation. expression of the macrophage phenotype is accompanied by increased levels of dna-binding activity and nuclear accumulation of wild-type p53. the dna-binding activity of the transcription ... | 1996 | 21544404 |
| differentiation of primary human fibroblasts into tissue macrophages by the snyder-theilen strain of the feline sarcoma virus is associated with endoreduplication, a 1,9 translocation, phenotypic stability, and apoptosis. | we have previously reported the conversion of human fibroblasts to tissue macrophages by transduction with the snyder-theilen feline sarcoma virus [st:fesv (felv)]. the st:fesv-induced macrophages were largely configured as spherical cells, each with an extensive network of microvilli. nuclear alterations included the appearance of three sub-populations within these mononucleated macrophages consisting of diploid (19%), tetraploid (43%), and octaploid (38%) cells. a karyotypic analysis revealed ... | 1996 | 21544487 |
| v-onc product expression in early phase post perinatal retrovirus infection predictive of malignancy of induced lesions. | sixteen sarcomas developed in rats within 40 days post perinatal infection with feline sarcoma virus of snyder-theilen strain were examined to elucidate the association of the viral oncogene product (p85) expression with malignancy of developed sarcomas. of these, 6 grew up to index-finger-tip-size, consisted of spindle shaped sarcoma cells and had no metastasis (group a), and 10 grew over thumb-tip-size, consisted of polygonal shaped sarcoma cells and had frequent metastasis (group b). one grou ... | 1997 | 21590093 |
| isolation of a transformation-defective mutant of the mcdonough strain of feline sarcoma virus exhibiting tyrosine kinase activity in vitro but not in vivo. | nrk cells transformed by the mcdonough strain of feline sarcoma virus (sm-fesv) were mutagenized by the use of 5'-azacytidine. four cell lines expressing different transformation-defective phenotypes were isolated. superinfection of these cell lines with simian sarcoma-associated virus (ssav) led in three instances to the recovery of transforming virus particles carrying an intact fms gene. a nonconditional transformation-defective virus, designated td26-sm-fesv (ssav), was isolated from one of ... | 2009 | 2835515 |
| isolation of an rd-114 related type-c virus from feline sarcoma virus-transformed baboon cells. | | 2014 | 4131819 |
| cellular regulation of mammalian sarcoma virus expression: a gene regulation model for oncogenesis. | investigations aimed at defining cellular functions required for expression of transformation by mammalian sarcoma viruses have led to the isolation of a class of revertants that contain biologically active feline sarcoma virus, yet possess in vitro and in vivo properties of normal cells. the block to expression of the transformed state in these cellular revertants was spontaneously reversible at low frequency. moreover, infection with certain helper viruses reversed the block at very high effic ... | 1978 | 222469 |
| annotated features of domestic cat - felis catus genome. | domestic cats enjoy an extensive veterinary medical surveillance which has described nearly 250 genetic diseases analogous to human disorders. feline infectious agents offer powerful natural models of deadly human diseases, which include feline immunodeficiency virus, feline sarcoma virus and feline leukemia virus. a rich veterinary literature of feline disease pathogenesis and the demonstration of a highly conserved ancestral mammal genome organization make the cat genome annotation a highly in ... | 2014 | 25143822 |
| body mass index is directly associated with biomarkers of angiogenesis and inflammation in children and adolescents. | childhood obesity is associated with an increased risk of atherosclerosis, which can be mediated by an increase in angiogenesis and inflammation. the objective was to investigate the association between body mass index (bmi) and circulating biomarkers of angiogenesis, inflammation, and cardiac dysfunction in children and adolescents. | 2012 | 22113066 |
| tyrosine 807 of the v-fms oncogene product controls cell morphology and association with p120rasgap. | expression of the v-fms oncogene of feline sarcoma virus in fibroblasts causes surface exposure of an activated receptor tyrosine kinase, v-fms, that is autophosphorylated at multiple sites within its cytoplasmic domain. cellular proteins interacting with this part of v-fms modulate the mitogenic activity and morphology of the cells. we show here that the tyrosine residue in position 807 (y-807) of the v-fms molecule constitutes a major autophosphorylation site. the replacement of this residue b ... | 1995 | 7666506 |
| influence of tyrosine residues y705 and y807 on the transforming potency of the v-fms oncogene product of feline sarcoma virus. | cell transformation is characterized by overt changes in growth control and cell morphology. to study the role of tyrosine residues y705 and y807 of v-fms of the mcdonough strain of feline sarcoma virus in cell transformation we replaced them individually with phenylalanine residues. cells expressing the mutant genes showed mitogenic properties similar to wild-type v-fms transformed cells. however, the morphology of cells expressing the y807f mutant remained the same as nontransformed cells. fou ... | 1995 | 7646343 |
| the actin domain of gardner-rasheed feline sarcoma virus inhibits kinase and transforming activities. | the transforming gene product, p70gag-actin-fgr, of gardner-rasheed feline sarcoma virus (gr-fesv) is a single polypeptide composed of regions derived from cellular and viral genes. gamma actin and c-fgr genes are the two known cellular components of the gr-fesv genome. in the present study, sequences representing each cell-derived gene were deleted and the resulting constructs were tested for transforming activity by transfection of nih 3t3 cells. constructs lacking a portion of the c-fgr proto ... | 1989 | 2538651 |
| the tp1 isolate of feline sarcoma virus encodes a fgr-related oncogene lacking gamma actin sequences. | we have isolated a new feline sarcoma virus, tp1-fesv. the virus encodes a myristilated 83 kd gag-onc fusion protein displaying tyrosine kinase activity. we have established nonproducer cell lines lacking the tp1-fesv associated helper virus (felv) and tp1-fesv transfected nih cell lines. southern blot analysis of genomic dna and northern blot analysis of rna isolated from these cell lines revealed that the oncogene of the tp1-fesv isolate is related to the fgr oncogene of the gr-fesv, but shows ... | 1989 | 2539576 |
| differential requirements of gag and gamma-actin domains for transforming potential of gardner-rasheed feline sarcoma virus. | the oncogene of gardner-rasheed feline sarcoma virus (gr-fesv) encodes the 70-kilodalton protein containing gag(p15), gamma-actin, and fgr domains. to determine the role of these domains in the biological activity of p70gag-actin-fgr, we have constructed in-frame deletion and insertion mutants of gr-fesv. we found, first, that the gamma-actin region could be deleted without affecting the transforming ability of these constructs, although an insertion mutant in the middle of the gamma-actin domai ... | 1989 | 2536827 |
| colony-stimulating factor-1 receptor (c-fms). | the macrophage colony-stimulating factor, csf-1 (m-csf), is a homodimeric glycoprotein required for the lineage-specific growth of cells of the mononuclear phagocyte series. apart from its role in stimulating the proliferation of bone marrow-derived precursors of monocytes and macrophages, csf-1 acts as a survival factor and primes mature macrophages to carry out differentiated functions. each of the actions of csf-1 are mediated through its binding to a single class of high-affinity receptors e ... | 1988 | 2852667 |
| a recessive cellular mutation in v-fes-transformed mink cells restores contact inhibition and anchorage-dependent growth. | a contact-inhibited revertant of mink cells transformed by the gardner-arnstein strain of feline sarcoma virus was isolated by fluorescence-activated sorting of cells stained with the mitochondria-specific dye rhodamine 123. the revertant cell line exhibited a decrease in its proliferative rate and saturation density and a complete loss of its capacity for anchorage-independent growth, but it remained tumorigenic when inoculated into nude mice. the revertant cells retained a rescuable gardner-ar ... | 1988 | 3261387 |
| correlation between csf-1 responsiveness and expression of (csf-1 receptor) c-fms in purified murine granulocyte-macrophage progenitor cells (cfu-gm). | the gene product of the viral proto-oncogene v-fms, associated with the feline sarcoma virus (sm-fesv), is a glycoprotein of 170 kd with associated tyrosine kinase activity. the murine c-fms proto-oncogene produces a similar glycoprotein of 165 kd and has been implicated as a similar, if not identical, molecule to the cell surface receptor of the hematopoietic growth factor csf-1. employing a v-fms probe in an in situ hybridization assay we examined the expression of c-fms transcripts in a popul ... | 1988 | 2962881 |
| correlation between csf-1 responsiveness and expression of (csf-1 receptor) c-fms in purified murine granulocyte-macrophage progenitor cells (cfu-gm). | the gene product of the viral proto-oncogene v-fms, associated with the feline sarcoma virus (sm-fesv), is a glycoprotein of 170 kd with associated tyrosine kinase activity. the murine c-fms proto-oncogene produces a similar glycoprotein of 165 kd and has been implicated as a similar, if not identical, molecule to the cell surface receptor of the hematopoietic growth factor csf-1. employing a v-fms probe in an in situ hybridization assay we examined the expression of c-fms transcripts in a popul ... | 1988 | 2826201 |
| structural analysis of a variant clone of snyder-theilen feline sarcoma virus. | a variant clone of snyder-theilen feline sarcoma virus (st-fesv) encoding a polyprotein with a molecular weight of approximately 104 kda (p104) was compared to the p85 encoding prototype clone of st-fesv. analysis of chimeric genes constructed with the viral oncogenes of the two clones indicated that the variant clone coded for a larger polyprotein than the prototype clone because of genetic differences in its 3' portion. comparative dna sequence analysis revealed that one nucleotide just upstre ... | 1987 | 2829460 |
| a new acute transforming feline retrovirus and relationship of its oncogene v-kit with the protein kinase gene family. | a new acute transforming feline retrovirus, the hardy-zuckerman 4 feline sarcoma virus (hz4-fesv), has been isolated from a feline fibrosarcoma. the viral genome of hz4-fesv contains a new oncogene designated v-kit, has the structure 5' delta gag-kit-delta pol-delta env 3' and specifies a gag-kit polyprotein of relative molecular mass 80,000. the predicted kit amino-acid sequence displays partial homology with tyrosine-specific protein kinase oncogenes. hz4-fesv appears to have been generated by ... | 2009 | 3007997 |
| cell surface expression of the mcdonough strain of feline sarcoma virus fms gene product (gp 140fms). | the unique oncogene carried by the mcdonough strain of feline sarcoma virus (sm-fesv), called v-fms, directs the synthesis of a set of related glycoproteins, called gp 180gag-fms, gp 140fms, and gp 120fms. we have prepared antibodies to these proteins and used indirect immunofluorescence techniques on viable sm-fesv transformed cells to demonstrate that fms-specific determinants are expressed on the external surface. the fms-specific fluorescence co-localized with clathrin and was detectable in ... | 1984 | 6094011 |
| subcellular localization of glycoproteins encoded by the viral oncogene v-fms. | the mcdonough strain of feline sarcoma virus encodes a polyprotein that is cotranslationally glycosylated and proteolytically cleaved to yield transforming glycoproteins specified by the viral oncogene v-fms. the major form of the glycoprotein (gp120fms) contains endoglycosidase h-sensitive, n-linked oligosaccharide chains lacking fucose and sialic acid, characteristic of glycoproteins in the endoplasmic reticulum. kinetic and steady-state measurements showed that most gp120fms molecules were no ... | 1984 | 6381756 |
| gene product of v-fgr onc: hybrid protein containing a portion of actin and a tyrosine-specific protein kinase. | the nucleotide sequence of the region of gardner-rasheed feline sarcoma virus (gr-fesv) encoding its primary translation product, p70gag-fgr, has been determined. from the nucleotide sequence, the amino acid sequence of this transforming protein was deduced. computer analysis indicates that a portion of p70gag-fgr has extensive amino acid sequence homology with actin, a eukaryotic cytoskeletal protein. a second region of p70gag-fgr is closely related to the tyrosine-specific kinase gene family. ... | 1984 | 6318314 |
| biochemical characterization of protein kinase activities associated with transforming gene products of the snyder-theilen and gardner-arnstein strains of feline sarcoma virus. | | 1982 | 6278734 |
| decreased uptake and retention of rhodamine 123 by mitochondria in feline sarcoma virus-transformed mink cells. | a reduce uptake and retention of the mitochondria-specific membrane potential probe rhodamine 123 by feline sarcoma virus (fesv)-transformed mink fibroblasts (64f3) has been detected. the decreased accumulation of rhodamine 123 by 64f3 mitochondria is not due to abnormal plasma membrane dye permeability, since after microinjection of the dye these cells are still unable to retain the dye at levels comparable to the untransformed parental cells, ccl 64. nigericin, an ionophore that mediates an el ... | 1982 | 6279315 |
| gene products of mcdonough feline sarcoma virus have an in vitro-associated protein kinase that phosphorylates tyrosine residues: lack of detection of this enzymatic activity in vivo. | the primary translational product of the mcdonough (sm) strain of feline sarcoma virus (fesv) is a 180,000-dalton molecule, sm p180, that contains the p15-p12-p30 region of the felv gag gene-coded precursor protein and a sarcoma virus-specific polypeptide. in addition, cells transformed by sm-fesv express a 120,000-dalton molecule, sm p120, that is highly related to the non-helper virus domain of sm p180. both sm-fesv gene products were found to be intimately associated with the membrane fractio ... | 1981 | 6275118 |
| transformation with subgenomic fragments of feline sarcoma virus proviral dna. | | 1981 | 6266138 |
| transformation-defective mutants of snyder-theilen feline sarcoma virus lack tyrosine-specific protein kinase activity. | four phenotypically normal mink cell clones, each containing a transformation-defective provirus of the snyder-theilen strain of feline sarcoma virus (st-fesv), synthesized an 85,000-dalton viral polyprotein (p85) indistinguishable in size and antigenic complexity from that encoded by wild-type transforming st-fesv. an additional transformation-defective, st-fesv-containing flat cell clone produced a polyprotein of 88,000 daltons (p88). the viral polyproteins immunoprecipitated from cytoplasmic ... | 1981 | 6168771 |
| snyder-theilen feline sarcoma virus p85 contains a single phosphotyrosine acceptor site recognized by its associated protein kinase. | cells nonproductively transformed by a variant of the snyder-theilen strain of feline sarcoma virus (fesv) expressed an 85,000-dalton polyprotein (p85) with associated tyrosine-specific protein kinase activity. we identified within this polyprotein a single tyrosine acceptor site for its enzyme activity. this acceptor site, as well as two serine phosphorylation sites localized with the p12 structural component of snyder-theilen fesv p85, was phosphorylated in cells nonproductively transformed by ... | 1981 | 6165840 |
| a transfection assay for transformation by feline sarcoma virus proviral dna. | | 1980 | 6245507 |
| isolation and characterization of a feline sarcoma virus-coded precursor polyprotein. competition immunoassay for nonstructural components. | | 1978 | 721821 |