| expression of the c-fms proto-oncogene during human monocytic differentiation. | the mcdonough strain of the feline sarcoma virus contains a transforming gene (v-fms) which contains partial nucleotide homology with proto-oncogenes encoding tyrosine kinases. one of the v-fms-encoded products, gp140fms, is a cell surface transmembrane glycoprotein that may function as a growth factor receptor. although c-fms transcripts have been detected in placental trophoblasts and normal human bone marrow, the role of the c-fms gene product is unknown. we now report that induction of monoc ... | 1985 | 2409450 |
| a dominant suppressive mutation in a cellular gene restores the nontransformed phenotype to v-fms-transformed mink cells. | a nontransformed revertant subclone (gvr17) was isolated from mutagen-treated populations of mink epithelial cells which had been transformed by the susan mcdonough strain of feline sarcoma virus (sm-fesv). the revertant cells, although contact-inhibited for growth and unable to form colonies in semisolid medium, contained two copies of integrated sm-fesv proviral dna, expressed high levels of the v-fms oncogene product and its associated tyrosine kinase activity, and yielded rescuable viruses a ... | 1991 | 2052353 |
| histiocytic conversion of human adult skin fibroblasts by the snyder-theilen feline sarcoma virus. | oncogenes are apparently involved in the transformation/neodifferentiation of human cells. we now report a novel example of transformation/neodifferentiation: the specific conversion of cultured human adult skin fibroblasts (hsf) to histiocytes (tissue macrophages (tm)) by the snyder-theilen feline sarcoma virus (st: (fesv)). the de novo conversion of cultured hsf was demonstrated in a large fraction of st:fesv (felv)-transformed foci in the presence of dexamethasone (dx). identification of tiss ... | 1990 | 1966584 |
| chromosomal assignment of the human homologues of feline sarcoma virus and avian myeloblastosis virus onc genes. | retroviral transforming genes, v-onc genes, are derived from normal cellular sequences that are called cellular onc (c-onc) genes. dna from mouse-human somatic cell hybrids that have selectively lost human chromosomes was used in southern blots to map the chromosomal location of two human onc genes. cloned human homologues of retroviral onc genes were used as probes. because the human c-fes gene, which is homologous to feline sarcoma virus, segregates concordantly with human chromosome 15, and t ... | 1982 | 6289315 |
| v-onc product expression in early phase post perinatal retrovirus infection predictive of malignancy of induced lesions. | sixteen sarcomas developed in rats within 40 days post perinatal infection with feline sarcoma virus of snyder-theilen strain were examined to elucidate the association of the viral oncogene product (p85) expression with malignancy of developed sarcomas. of these, 6 grew up to index-finger-tip-size, consisted of spindle shaped sarcoma cells and had no metastasis (group a), and 10 grew over thumb-tip-size, consisted of polygonal shaped sarcoma cells and had frequent metastasis (group b). one grou ... | 1997 | 21590093 |
| isolation of human oncogene sequences (v-fes homolog) from a cosmid library. | to define the human homolog (or homologs) of transforming sequences (v-fes gene) common to gardner (ga) and snyder theilen (st) isolates of feline sarcoma virus (fesv), a representative library of human lung carcinoma dna in a cosmid vector system was constructed. three cosmid clones were isolated containing ga/st fesv v-fes homologous cellular sequences, within 32- to 42-kilobase cellular inserts representing 56 kilobases of contiguous human cellular dna. sequences both homologous to, and colin ... | 1982 | 6281890 |
| isolation and characterization of a human locus homologous to the transforming gene (v-fes) of feline sarcoma virus. | a single locus (designated c-fes) in the human genome which exhibits homology to the transformation-specific onc gene (v-fes) of snyder-theilen feline sarcoma virus was identified by the southern blot technique. recombinant clones containing 16- to 18-kilobase inserts of human dna including the c-fes locus were constructed. restriction endonuclease mapping of these clones verified their identity with native human c-fes and demonstrated the presence of at least two sequences in human c-fes interr ... | 1982 | 6277911 |
| biochemical characterization of cells transformed via transfection by feline sarcoma virus proviral dna. | murine fibroblasts transformed by transfection with dna from mink cells infected with the snyder-theilen strain of feline sarcoma virus and subgroup b feline leukemia virus were analyzed for the presence of integrated proviral dna and the expression of feline leukemia virus- and feline sarcoma virus-specific proteins. the transformed murine cells harbored at least one intact feline sarcoma virus provirus, but did not contain feline leukemia virus provirus. the transformed murine cells expressed ... | 1981 | 6264144 |
| annotated features of domestic cat - felis catus genome. | domestic cats enjoy an extensive veterinary medical surveillance which has described nearly 250 genetic diseases analogous to human disorders. feline infectious agents offer powerful natural models of deadly human diseases, which include feline immunodeficiency virus, feline sarcoma virus and feline leukemia virus. a rich veterinary literature of feline disease pathogenesis and the demonstration of a highly conserved ancestral mammal genome organization make the cat genome annotation a highly in ... | 2014 | 25143822 |
| involvement of a high-molecular-weight polyprotein translational product of snyder-theilen feline sarcoma virus in malignant transformation. | the previously described high-molecular-weight polyprotein major translational product of the snyder-theilen strain of feline sarcoma virus (fesv) was shown to possess protein kinase activity with specificity for tyrosine acceptor sites. cells transformed by snyder-theilen fesv exhibited constitutively elevated levels of phosphotyrosine and a concomitant reduction in epidermal growth factor (egf) binding sites. by endpoint cloning in microtiter plates, a number of transformation-defective (tf) m ... | 1981 | 6261011 |
| translational products encoded by newly acquired sequences of independently derived feline sarcoma virus isolates are structurally related. | polyproteins encoded by several independent isolates of feline sarcoma virus (fesv) were analyzed with respect to molecular weight, extent of phosphorylation, and tryptic peptide composition. as previously reported, cells nonproductively transformed by the gardner strain of fesv express a polyprotein which has a molecular weight of approximately 115,000 and contains feline leukemia virus p15, p12, and minor portion of p30. in addition, a major 72,000-dalton possible cleavage product can be ident ... | 1980 | 6245259 |
| cytotoxic immune response of puppies to feline sarcoma virus induced tumors. | the cytotoxic immune response of puppies to feline sarcoma virus induced tumors was studied. neonatal puppies were compared with adolescent dogs. three different types of cytotoxicity were investigated: complement dependent cytotoxicity, t-cell-mediated cytotoxicity and antibody dependent cellular cytotoxicity. the relationship between the spontaneous regression of the sarcoma and the development of the immune system of the puppies is discussed. | 1984 | 6093343 |
| type beta transforming growth factor from feline sarcoma virus-transformed rat cells. isolation and biological properties. | we have isolated a strongly mitogenic, type beta transforming growth factor (beta tgf) released by snyder-theilen feline sarcoma virus-transformed rat embryo (fesv-fre) cells that induces phenotypic transformation of normal nrk cells when they are concomitantly stimulated by analogues of epidermal growth factor (egf). molecule filtration chromatography separates beta tgf from an egf-like tgf (etgf) which is also present in acid extracts from medium conditioned by fesv-fre cells (j. massagué, (19 ... | 1984 | 6086646 |
| human gene (c-fes) related to the onc sequences of snyder-theilen feline sarcoma virus. | the onc gene (v-fes) of the acutely transforming feline sarcoma virus (snyder-theilen strain) has homologous cellular sequences (c-fes) in all vertebrate species, including humans. we isolated from a human dna library recombinant phages containing overlapping c-fes sequences. the human c-fes locus spans a region of 3.4 kilobases and contains 1.4 kilobases of dna homologous to the viral onc sequence interspersed with three intervening sequences. | 1982 | 6290870 |
| the parodi-irgens feline sarcoma virus and simian sarcoma virus have homologous oncogenes, but in different contexts of the viral genomes. | we have identified the oncogene and the putative transforming protein of the parodi-irgens feline sarcoma virus (pi-fesv). the pi-fesv is defective and needs a helper virus for its replication. the v-onc sequences in the pi-fesv were found to be related to the v-sis sequences of the simian sarcoma virus (ssv). pi-fesv nonproducer cells express two viral rnas, a 6.8-and a 3.3-kilobase rna. the 6.8-kilobase rna contains gag, sis, and env sequences but lacks the pol gene. the 3.3-kilobase rna, on t ... | 1983 | 6302323 |
| monoclonal antibodies to feline sarcoma virus gag and fes gene translational products. | a series of hybridomas have been isolated which produce monoclonal antibodies directed against polyprotein gene products of the gardner, snyder-theilen, and mcdonough strains of fesv. within these are representatives of several immunoglobulin classes including igg1, igg2a, igg2b, and igm. antibody produced by one hybridoma recognizes immunologic determinants localized within an felv gag gene structural component (p15) common to polyproteins encoded by all three fesv isolates whereas antibody pro ... | 1983 | 6308016 |
| molecular cloning of integrated gardner-rasheed feline sarcoma virus: genetic structure of its cell-derived sequence differs from that of other tyrosine kinase-coding onc genes. | gardner-rasheed feline sarcoma virus (gr-fesv) is an acute transforming retrovirus which encodes a gag-onc polyprotein possessing an associated tyrosine kinase activity. the integrated form of this virus, isolated in the charon 21a strain of bacteriophage lambda, demonstrated an ability to transform nih/3t3 cells at high efficiency upon transfection. foci induced by gr-fesv dna contained rescuable sarcoma virus and expressed gr-p70, the major gr-fesv translational product. the localization of lo ... | 1983 | 6312085 |
| antibodies of predetermined specificity detect two retroviral oncogene products and inhibit their kinase activities. | oligopeptides predicted from the nucleotide sequence of the oncogene v-fes of feline sarcoma virus (fesv) were synthesized chemically and used to generate specific antibodies. antisera against a 12-amino-acid-long oligopeptide (12-mer) located 42 residues from the carboxyl terminus of the v-fes coding sequence efficiently recognized the transforming proteins encoded by snyder-theilen (st) and gardner-arnstein (ga) strains of fesv. this 12-mer also contains 10 amino acid residues homologous in or ... | 1983 | 6572383 |
| epidermal growth factor-like transforming growth factor. i. isolation, chemical characterization, and potentiation by other transforming factors from feline sarcoma virus-transformed rat cells. | an acid-stable transforming growth factor (tgf) that interacts with epidermal growth factor (egf) receptors and is structurally related to egf was isolated from serum-free culture fluids of snyder-theilen feline sarcoma virus-transformed rat embryo (fesv-fre) cells. purification of this egf-like tgf (etgf) was achieved by molecular filtration chromatography and successive reverse-phase high pressure liquid chromatography steps on octadecyl support eluted with acetonitrile and 1-propanol gradient ... | 1983 | 6605968 |
| chromosomal sublocalization of human c-myb and c-fes cellular onc genes. | the transforming genes of acutely transforming retroviruses are derived from conserved cellular genes (c-onc genes) which are believed to be important in normal cell growth and differentiation. recent studies indicate that altered expression of c-onc genes, for example, by insertion of viral genomes, gene amplification or chromosomal translocation, can lead to development of malignant diseases in man and animals. c-myb and c-fes are homologues of the transforming genes of avian myeloblastosis vi ... | 1983 | 6866112 |
| malignant transformation of a human fibroblast cell strain by transfection of a v-fes oncogene but not by transfection of a gag-human c-fes construct. | to determine whether the human c-fes gene, a homologue of the feline v-fes oncogene, can play a role in the malignant transformation of human fibroblasts, we transfected a near-diploid, infinite life span, growth factor-independent, human fibroblast cell strain, msu-1.2, with plasmids carrying a fes gene along with a selectable marker. the fes gene was either the v-fes oncogene from the gardner-arnstein strain of feline sarcoma virus or a chimeric construct in which 835 base pairs representing e ... | 1994 | 7696187 |
| suppression of retroviral tumors in hosts immune to viral oncogene product. | feline sarcoma virus of snyder-theilen strain (st-fesv) induces sarcomas in wistar/ma rats following neonatal virus injection. induced tumors express the viral oncogene product (p85) and elicit in hosts the specific serum anti-p85 antibody detectable by western blot analysis. syngeneic adult female rats were immunized with an st-fesv induced sarcoma that was 100% transplantable to syngeneic adult rats. newborns from immunized rats (vaccinated rats) were found to carry anti-p85 in their sera at b ... | 1994 | 8152290 |
| conversion of human fibroblasts to tissue macrophages by the snyder-theilen sarcoma virus (st:fesv): tumouricidal effects on k-562 and daudi tumour cell lines in monolayers and in agar suspensions. | we have previously demonstrated the conversion of human fibroblasts (hf) to tissue macrophages by transduction with the snyder-theilen feline sarcoma virus (st:fesv) [1-3]. the st:fesv-induced tm have been characterized both phenotypically and functionally, including their tumouricidal potential against colon adenocarcinoma (ls 180) cells. the present results show that st:fesv-induced tm produced significant lysis of k-562 tumour cells, but essentially no lysis of the daudi tumour cells. lysis o ... | 1993 | 8268416 |
| differentiation of human fibroblasts to tissue macrophages by the snyder-theilen feline sarcoma virus (st:fesv): growth modulation of human tumor cell lines in agar. | snyder-theilen feline sarcoma virus (st:fesv)-transduced human fibroblasts differentiate into tissue macrophages(1-9). the st:fesv-induced macrophages demonstrate macrophage-mediated cytotoxicity (mtc) and antibody-dependent cellular cytotoxicity (adcc), including growth modulation of tumor cells in agar (3,4,6). | 1997 | 9252687 |
| c-cbl associates directly with the c-terminal tail of the receptor for the macrophage colony-stimulating factor, c-fms, and down-modulates this receptor but not the viral oncogene v-fms. | the receptor for the macrophage colony-stimulating factor (csf-1, also termed m-csf), the tyrosine kinase c-fms, was originally determined to be the oncogene product of the mcdonough strain of feline sarcoma virus, v-fms. the structural difference between c-fms and v-fms amounts to only five point mutations in the extracellular domain, two mutations in the cytoplasmic domain, and the replacement of 50 amino acids by 14 unrelated amino acids at the c-terminal tail. here, we have identified c-cbl ... | 2002 | 11847211 |
| effects of the cfms kinase inhibitor 5-(3-methoxy-4-((4-methoxybenzyl)oxy)benzyl)pyrimidine-2,4-diamine (gw2580) in normal and arthritic rats. | the cfms (cellular homolog of the v-fms oncogene product of the susan mcdonough strain of feline sarcoma virus) (proc natl acad sci u s a 83:3331-3335, 1986) kinase inhibitor 5-(3-methoxy-4-((4-methoxybenzyl)oxy)benzyl)pyrimidine-2,4-diamine (gw2580) inhibits colony-stimulating factor (csf)-1-induced monocyte growth and bone degradation in vitro and inhibits csf-1 signaling through cfms kinase in 4-day models in mice (proc natl acad sci u s a 102:16078, 2005). in the present study, the kinase se ... | 2008 | 18434589 |
| viral gene mutation as determinant of low grade malignancy of lesions induced by perinatal retrovirus infection. | of 108 wistar/ma rats perinatally injected with snyder-theilen feline sarcoma virus, 75 developed tumors within 60 days (group a), and 25 after more than 117 days (group b). metastases were detected in 59.1% and 11.8% in group a and b respectively. antibody to v-fes gene product was found to occur before tumor development in group b and after tumor development in group a. v-ses was found to be down-regulated and extensively mutated in tumors of group b. these results suggest viral gene mutation ... | 1997 | 21533410 |
| differentiation of human fibroblasts to tissue macrophages by the snyder-theilen feline sarcoma virus (st:fesv). | we have previously reported the conversion of human fibroblasts (hf) to tissue macrophages (tm) by transduction with the snyder-theilen feline sarcoma virus [st:fesv(felv)]. here, we have demonstrated macrophage mediated tumor cytotoxicity (mtc) of human teratocarcinoma cells (ntera-2) by st:fesv-induced tm. inclusion of the ntera-2 specific antibody (ssea-4), substantially increased antibody-dependent cellular cytotoxicity (adcc) of the tumor cells by st:fesv-induced tm. tumor cell lysis by st: ... | 1996 | 21544403 |
| differentiation of human fibroblasts to tissue macrophages by the snyder-theilen feline sarcoma virus is accompanied by nuclear accumulation of the tumor suppressor p53. | snyder-theilen feline sarcoma virus (st:fesv)-transduced human fibroblasts differentiate into tissue macrophages with many of the properties of normal macrophages. these cells express high levels of the gag-fes tyrosine kinase fusion protein, p85(v-fes), and exhibit an elevated level of tyrosine phosphorylation. expression of the macrophage phenotype is accompanied by increased levels of dna-binding activity and nuclear accumulation of wild-type p53. the dna-binding activity of the transcription ... | 1996 | 21544404 |
| isolation of a transformation-defective mutant of the mcdonough strain of feline sarcoma virus exhibiting tyrosine kinase activity in vitro but not in vivo. | nrk cells transformed by the mcdonough strain of feline sarcoma virus (sm-fesv) were mutagenized by the use of 5'-azacytidine. four cell lines expressing different transformation-defective phenotypes were isolated. superinfection of these cell lines with simian sarcoma-associated virus (ssav) led in three instances to the recovery of transforming virus particles carrying an intact fms gene. a nonconditional transformation-defective virus, designated td26-sm-fesv (ssav), was isolated from one of ... | 2009 | 2835515 |
| isolation of an rd-114 related type-c virus from feline sarcoma virus-transformed baboon cells. | | 2014 | 4131819 |
| isolation and characterization of a feline sarcoma virus-coded precursor polyprotein. competition immunoassay for nonstructural components. | | 1978 | 721821 |
| cellular regulation of mammalian sarcoma virus expression: a gene regulation model for oncogenesis. | investigations aimed at defining cellular functions required for expression of transformation by mammalian sarcoma viruses have led to the isolation of a class of revertants that contain biologically active feline sarcoma virus, yet possess in vitro and in vivo properties of normal cells. the block to expression of the transformed state in these cellular revertants was spontaneously reversible at low frequency. moreover, infection with certain helper viruses reversed the block at very high effic ... | 1978 | 222469 |
| body mass index is directly associated with biomarkers of angiogenesis and inflammation in children and adolescents. | childhood obesity is associated with an increased risk of atherosclerosis, which can be mediated by an increase in angiogenesis and inflammation. the objective was to investigate the association between body mass index (bmi) and circulating biomarkers of angiogenesis, inflammation, and cardiac dysfunction in children and adolescents. | 2012 | 22113066 |
| tyrosine 807 of the v-fms oncogene product controls cell morphology and association with p120rasgap. | expression of the v-fms oncogene of feline sarcoma virus in fibroblasts causes surface exposure of an activated receptor tyrosine kinase, v-fms, that is autophosphorylated at multiple sites within its cytoplasmic domain. cellular proteins interacting with this part of v-fms modulate the mitogenic activity and morphology of the cells. we show here that the tyrosine residue in position 807 (y-807) of the v-fms molecule constitutes a major autophosphorylation site. the replacement of this residue b ... | 1995 | 7666506 |
| influence of tyrosine residues y705 and y807 on the transforming potency of the v-fms oncogene product of feline sarcoma virus. | cell transformation is characterized by overt changes in growth control and cell morphology. to study the role of tyrosine residues y705 and y807 of v-fms of the mcdonough strain of feline sarcoma virus in cell transformation we replaced them individually with phenylalanine residues. cells expressing the mutant genes showed mitogenic properties similar to wild-type v-fms transformed cells. however, the morphology of cells expressing the y807f mutant remained the same as nontransformed cells. fou ... | 1995 | 7646343 |
| the actin domain of gardner-rasheed feline sarcoma virus inhibits kinase and transforming activities. | the transforming gene product, p70gag-actin-fgr, of gardner-rasheed feline sarcoma virus (gr-fesv) is a single polypeptide composed of regions derived from cellular and viral genes. gamma actin and c-fgr genes are the two known cellular components of the gr-fesv genome. in the present study, sequences representing each cell-derived gene were deleted and the resulting constructs were tested for transforming activity by transfection of nih 3t3 cells. constructs lacking a portion of the c-fgr proto ... | 1989 | 2538651 |
| the tp1 isolate of feline sarcoma virus encodes a fgr-related oncogene lacking gamma actin sequences. | we have isolated a new feline sarcoma virus, tp1-fesv. the virus encodes a myristilated 83 kd gag-onc fusion protein displaying tyrosine kinase activity. we have established nonproducer cell lines lacking the tp1-fesv associated helper virus (felv) and tp1-fesv transfected nih cell lines. southern blot analysis of genomic dna and northern blot analysis of rna isolated from these cell lines revealed that the oncogene of the tp1-fesv isolate is related to the fgr oncogene of the gr-fesv, but shows ... | 1989 | 2539576 |
| differential requirements of gag and gamma-actin domains for transforming potential of gardner-rasheed feline sarcoma virus. | the oncogene of gardner-rasheed feline sarcoma virus (gr-fesv) encodes the 70-kilodalton protein containing gag(p15), gamma-actin, and fgr domains. to determine the role of these domains in the biological activity of p70gag-actin-fgr, we have constructed in-frame deletion and insertion mutants of gr-fesv. we found, first, that the gamma-actin region could be deleted without affecting the transforming ability of these constructs, although an insertion mutant in the middle of the gamma-actin domai ... | 1989 | 2536827 |
| colony-stimulating factor-1 receptor (c-fms). | the macrophage colony-stimulating factor, csf-1 (m-csf), is a homodimeric glycoprotein required for the lineage-specific growth of cells of the mononuclear phagocyte series. apart from its role in stimulating the proliferation of bone marrow-derived precursors of monocytes and macrophages, csf-1 acts as a survival factor and primes mature macrophages to carry out differentiated functions. each of the actions of csf-1 are mediated through its binding to a single class of high-affinity receptors e ... | 1988 | 2852667 |
| a recessive cellular mutation in v-fes-transformed mink cells restores contact inhibition and anchorage-dependent growth. | a contact-inhibited revertant of mink cells transformed by the gardner-arnstein strain of feline sarcoma virus was isolated by fluorescence-activated sorting of cells stained with the mitochondria-specific dye rhodamine 123. the revertant cell line exhibited a decrease in its proliferative rate and saturation density and a complete loss of its capacity for anchorage-independent growth, but it remained tumorigenic when inoculated into nude mice. the revertant cells retained a rescuable gardner-ar ... | 1988 | 3261387 |
| correlation between csf-1 responsiveness and expression of (csf-1 receptor) c-fms in purified murine granulocyte-macrophage progenitor cells (cfu-gm). | the gene product of the viral proto-oncogene v-fms, associated with the feline sarcoma virus (sm-fesv), is a glycoprotein of 170 kd with associated tyrosine kinase activity. the murine c-fms proto-oncogene produces a similar glycoprotein of 165 kd and has been implicated as a similar, if not identical, molecule to the cell surface receptor of the hematopoietic growth factor csf-1. employing a v-fms probe in an in situ hybridization assay we examined the expression of c-fms transcripts in a popul ... | 1988 | 2962881 |
| correlation between csf-1 responsiveness and expression of (csf-1 receptor) c-fms in purified murine granulocyte-macrophage progenitor cells (cfu-gm). | the gene product of the viral proto-oncogene v-fms, associated with the feline sarcoma virus (sm-fesv), is a glycoprotein of 170 kd with associated tyrosine kinase activity. the murine c-fms proto-oncogene produces a similar glycoprotein of 165 kd and has been implicated as a similar, if not identical, molecule to the cell surface receptor of the hematopoietic growth factor csf-1. employing a v-fms probe in an in situ hybridization assay we examined the expression of c-fms transcripts in a popul ... | 1988 | 2826201 |
| structural analysis of a variant clone of snyder-theilen feline sarcoma virus. | a variant clone of snyder-theilen feline sarcoma virus (st-fesv) encoding a polyprotein with a molecular weight of approximately 104 kda (p104) was compared to the p85 encoding prototype clone of st-fesv. analysis of chimeric genes constructed with the viral oncogenes of the two clones indicated that the variant clone coded for a larger polyprotein than the prototype clone because of genetic differences in its 3' portion. comparative dna sequence analysis revealed that one nucleotide just upstre ... | 1987 | 2829460 |
| a new acute transforming feline retrovirus and relationship of its oncogene v-kit with the protein kinase gene family. | a new acute transforming feline retrovirus, the hardy-zuckerman 4 feline sarcoma virus (hz4-fesv), has been isolated from a feline fibrosarcoma. the viral genome of hz4-fesv contains a new oncogene designated v-kit, has the structure 5' delta gag-kit-delta pol-delta env 3' and specifies a gag-kit polyprotein of relative molecular mass 80,000. the predicted kit amino-acid sequence displays partial homology with tyrosine-specific protein kinase oncogenes. hz4-fesv appears to have been generated by ... | 2009 | 3007997 |
| cell surface expression of the mcdonough strain of feline sarcoma virus fms gene product (gp 140fms). | the unique oncogene carried by the mcdonough strain of feline sarcoma virus (sm-fesv), called v-fms, directs the synthesis of a set of related glycoproteins, called gp 180gag-fms, gp 140fms, and gp 120fms. we have prepared antibodies to these proteins and used indirect immunofluorescence techniques on viable sm-fesv transformed cells to demonstrate that fms-specific determinants are expressed on the external surface. the fms-specific fluorescence co-localized with clathrin and was detectable in ... | 1984 | 6094011 |
| subcellular localization of glycoproteins encoded by the viral oncogene v-fms. | the mcdonough strain of feline sarcoma virus encodes a polyprotein that is cotranslationally glycosylated and proteolytically cleaved to yield transforming glycoproteins specified by the viral oncogene v-fms. the major form of the glycoprotein (gp120fms) contains endoglycosidase h-sensitive, n-linked oligosaccharide chains lacking fucose and sialic acid, characteristic of glycoproteins in the endoplasmic reticulum. kinetic and steady-state measurements showed that most gp120fms molecules were no ... | 1984 | 6381756 |
| gene product of v-fgr onc: hybrid protein containing a portion of actin and a tyrosine-specific protein kinase. | the nucleotide sequence of the region of gardner-rasheed feline sarcoma virus (gr-fesv) encoding its primary translation product, p70gag-fgr, has been determined. from the nucleotide sequence, the amino acid sequence of this transforming protein was deduced. computer analysis indicates that a portion of p70gag-fgr has extensive amino acid sequence homology with actin, a eukaryotic cytoskeletal protein. a second region of p70gag-fgr is closely related to the tyrosine-specific kinase gene family. ... | 1984 | 6318314 |
| biochemical characterization of protein kinase activities associated with transforming gene products of the snyder-theilen and gardner-arnstein strains of feline sarcoma virus. | | 1982 | 6278734 |
| decreased uptake and retention of rhodamine 123 by mitochondria in feline sarcoma virus-transformed mink cells. | a reduce uptake and retention of the mitochondria-specific membrane potential probe rhodamine 123 by feline sarcoma virus (fesv)-transformed mink fibroblasts (64f3) has been detected. the decreased accumulation of rhodamine 123 by 64f3 mitochondria is not due to abnormal plasma membrane dye permeability, since after microinjection of the dye these cells are still unable to retain the dye at levels comparable to the untransformed parental cells, ccl 64. nigericin, an ionophore that mediates an el ... | 1982 | 6279315 |
| gene products of mcdonough feline sarcoma virus have an in vitro-associated protein kinase that phosphorylates tyrosine residues: lack of detection of this enzymatic activity in vivo. | the primary translational product of the mcdonough (sm) strain of feline sarcoma virus (fesv) is a 180,000-dalton molecule, sm p180, that contains the p15-p12-p30 region of the felv gag gene-coded precursor protein and a sarcoma virus-specific polypeptide. in addition, cells transformed by sm-fesv express a 120,000-dalton molecule, sm p120, that is highly related to the non-helper virus domain of sm p180. both sm-fesv gene products were found to be intimately associated with the membrane fractio ... | 1981 | 6275118 |
| transformation with subgenomic fragments of feline sarcoma virus proviral dna. | | 1981 | 6266138 |
| differentiation of primary human fibroblasts into tissue macrophages by the snyder-theilen strain of the feline sarcoma virus is associated with endoreduplication, a 1,9 translocation, phenotypic stability, and apoptosis. | we have previously reported the conversion of human fibroblasts to tissue macrophages by transduction with the snyder-theilen feline sarcoma virus [st:fesv (felv)]. the st:fesv-induced macrophages were largely configured as spherical cells, each with an extensive network of microvilli. nuclear alterations included the appearance of three sub-populations within these mononucleated macrophages consisting of diploid (19%), tetraploid (43%), and octaploid (38%) cells. a karyotypic analysis revealed ... | 1996 | 21544487 |
| a transfection assay for transformation by feline sarcoma virus proviral dna. | | 1980 | 6245507 |
| transformation-defective mutants of snyder-theilen feline sarcoma virus lack tyrosine-specific protein kinase activity. | four phenotypically normal mink cell clones, each containing a transformation-defective provirus of the snyder-theilen strain of feline sarcoma virus (st-fesv), synthesized an 85,000-dalton viral polyprotein (p85) indistinguishable in size and antigenic complexity from that encoded by wild-type transforming st-fesv. an additional transformation-defective, st-fesv-containing flat cell clone produced a polyprotein of 88,000 daltons (p88). the viral polyproteins immunoprecipitated from cytoplasmic ... | 1981 | 6168771 |
| snyder-theilen feline sarcoma virus p85 contains a single phosphotyrosine acceptor site recognized by its associated protein kinase. | cells nonproductively transformed by a variant of the snyder-theilen strain of feline sarcoma virus (fesv) expressed an 85,000-dalton polyprotein (p85) with associated tyrosine-specific protein kinase activity. we identified within this polyprotein a single tyrosine acceptor site for its enzyme activity. this acceptor site, as well as two serine phosphorylation sites localized with the p12 structural component of snyder-theilen fesv p85, was phosphorylated in cells nonproductively transformed by ... | 1981 | 6165840 |