| [expression of individual proteins of mouse mammary gland cancer virus in tumors in mice of various strains]. | expression of the main structural proteins of mammary gland cancer virus, gp52 and p27, was studied with the immune sera to these proteins in homogenates of tumors from mice of low and high cancer lines. protein gp52 was found only in tumors of mice-carriers of mtv-s. in a fraction of purified type a particles from the cytoplasm of mammary gland cancer no gp52 was found, but with the antiserum to p27 this fraction reacted by a line identical to that with the antigen from c2hf mouse tumor. | 1976 | 188255 |
| [effect of rna on tumor growth in mice of lines with and without the milk factor]. | | 1977 | 188616 |
| steroid induction of mouse mammary tumor virus: effect upon synthesis and degradation of viral rna. | steroid hormones have been demonstrated to induce in tissue culture the production of mouse mammary tumor viral (mmtv) rna, proteins, and particles 10-fold compared with constitutive levels. however, previous data of increased viral rna levels did not distinguish between an increased rate of viral-specific rna synthesis and a slower rate of viral rna degradation. according to the recently developed assay of coffin et al. (1974) for measuring rates of viral rna synthesis, short-term labeling expe ... | 1977 | 189061 |
| application of freeze-drying intact cells to studies of murine oncornavirus morphogenesis. | using a method for freeze-drying intact cells, uninfected and murine leukemia virus (mulv)-infected jlsv9 cell surfaces, as well as murine mammary tumor virus (mumtv)-infected cell surfaces, were examined by electron microscopy. the 10-nm knobs of mulv and the 5-nm spikes of mumtv were clearly revealed on the surfaces of budding viruses and were also found dispersed over the cell surface. the mulv knobs are randomly arranged on the virus surface, whereas the mumtv spikes are much more ordered. b ... | 1977 | 189070 |
| cyclic amp regulation of mammary tumor virus production. | addition of dibutyryl cyclic amp (camp) and agents (isoproterenol and epinephrine) that stimulate the activities of adenylate cyclase enhance the stimulation of mammary tumor virus (mtv) production two- to threefold by glucocorticoid in short-term primary cultures of mammary tumors. this camp potentiation seems to depend on the stimulated level of mtv production by glucocorticoid alone, which increases mtv production 5- to 10-fold over basal level but varies greatly in absolute terms. when the ... | 1977 | 189095 |
| rna tumor viruses and breast cancer. | | 1976 | 189374 |
| immunology: breast cancer. | | 1976 | 189376 |
| infection of cultured rat hepatoma cells by mouse mammary tumor virus. | a continuous line of buffalo rat hepatoma (htc) cells has been successfully infected with mouse mammary tumor virus (mmtv) produced by the gr mammary tumor cell line. uniform infection required initial exposure of the htc cells to greater than 10(5) mmtv particles per cell. the resultant chronically infected cell population was found to have stably acquired 20-30 copies of mmtv dna. the infected cells contain viral rna and express viral antigens; however, very few mmtv particles are released int ... | 1977 | 189932 |
| mouse mammary tumor virus dna in infected rat cells: characterization of unintegrated forms. | mouse mammary tumor virus (mmtv) dna in chronically infected rat hepatoma cells is maintained in both the integrated and unintegrated state. fractionation of dna by the procedure of hirt (1967) as well as by sedimentation through alkaline sucrose suggests that about two thirds of the viral dna is associated with high molecular weight cell dna. the remainder of the viral dna is unintegrated and is present primarily as linear or open circular duplexes consisting of a genome-length strand complemen ... | 1977 | 189933 |
| inhibition of mammary tumors by incomplete t-cell depletion. | the effects of neonatal and perinatal thymectomy on mammary tumorigenesis in (c57bl x i)f1fc3h hybrid female mice were determined. when hybrid females were neonatally thymectomized by controlled suction, a procedure that removes thymic lobes completely, a large proportion of animals developed stigmas of a fulminant wasting disease and died before tumors developed. however, when hybrid females were subjected to neonatal thymectomy by continuous suction, a procedure that resulted in retention of t ... | 1977 | 190416 |
| antibody reacting with mouse mammary tumor virus in serum of breast carcinoma patients. | | 1976 | 190717 |
| structural components of mouse mammary tumor virus. i. polypeptides of the virion. | | 1977 | 190773 |
| replication of mouse mammary tumor virus in tissue culture. ii. kinetics of virus production and the effect of rna and protein inhibitors on viral synthesis. | | 1977 | 190785 |
| the role of leukocyte subpopulations in the indirect leukocyte adherence inhibition assay in the mammary tumor virus system. | a modification of the leukocyte adherence inhibition test for the detection of cellular immunologic reactivity of mice to the mammary tumor virus (mtv) has been described. it involves the transfer of the leukocyte adherence inhibition factor (laif) produced by spleen cells from immunized animals when cultured with antigen to indicator cells, for which peritoneal exudate cells from normal mice are used. the method proves to be sensitive and highly reproducible. by crude separation of leukocyte su ... | 1977 | 191259 |
| genetic transmission of mammary tumour virus by gr mice. | by immunodiffusion assay (id-test) milk samples of mice of several strains and of f1-hybrids of the gr strain were tested for the presence of mammary tumour virus (mtv) antigens. the results clearly demonstrated that the presence of viral antigens in the milk of the first lactation period is restricted to mice harbouring endogenous mtv-gr. viral antigens were detectable in about 50% of the milk samples collected during the first (occasionally the second) lactation periods of mice of the segregat ... | 1977 | 191409 |
| naturally occurring lymphocyte reactivity to purified mammary tumor virus antigens. | | 1977 | 191607 |
| natural antibody in mammary tumor virus-infected mice that reacts with intracytoplasmic a particles of mouse mammary tumors. | by an indirect immunofluorescence technique with prolonged serum incubation on murine mammary tumor (mt) slices, 179 of 424 mice examined were found to possess natural serum antibody (antibodies) that reacted with intracytoplasmic a particles (iap) of mt cells. the immunologic specificity of this antibody was supported by absorption and blocking experiments. furthermore, a strong similarity was seen between the mouse antibody reaction on various mt and the fluorescence pattern of rabbit anti-iap ... | 1977 | 191630 |
| isolation of the mouse mammary tumor virus sequences not transmitted as germinal provirus in the c3h and riii mouse strains. | radioactive 60-70s rna from the mouse mammary tumor virus (mmtv) produced by the c3h mouse mammary tumor cell line (mm5mt) hybridized to a greater extent, and at a lower cot1/2 value, to the dna of c3h mammary tumor cells than to the dna of c3h liver cells. the 125i-labeled mmtv (c3h) 60-40s rna was annealed to a vast excess of dna from c3h livers, and single-stranded rna was eluted from hydroxylapatite and recovered. this "recycled rna" did not hybridize to the dna of the apparently normal orga ... | 1977 | 191657 |
| procedures for radioimmunoassay of the mouse mammary tumor virus. | a procedure for radioimmunoassay of the major glycoprotein antigen derived from murine mammary tumor virus is described. the assay is sensitive to 0.05 ng of antigen and is highly reproducible. the antigen, gp55, has been found to be group specific and will detect viruses in 13 separate mouse strains, as wel .l as from continuous cell lines. factors affecting the assay have been examined. | 1977 | 192451 |
| plasma levels of a viral protein as a diagnostic signal for the presence of mammary tumor: the effect of tumor removal. | we have previously shown (1, 2) that mice with mammary tumors can always be identified by their very high plasma levels of gp52, a 52,000 mol wt glycoprotein of the mouse mammary tumor virus (mmtv). the present investigation demostrates that the tumor is the principal source of the plasma gp52 since surgical excision is invariably followed in the first 9 days by a sharp decreasing (10-100-fold) of the gp52 levels. control animals in which the tumors were left in place by a "sham" surgical proced ... | 1977 | 192830 |
| the role of oncogenic viruses in neoplasia. | | 1977 | 193432 |
| levels of mammary tumor virus in hormone-dependent and -independent mouse mammary tumor cells. | levels of mammary tumor virus particles (types a and b) and levels of the virus antigen were assayed in hormone-dependent and -independent mammary tumors of gr mice. various transplant generations of seven separate tumor lines were investigated. the results indicated that the tumors consisted of different cell clones, each of which exhibited a separate progressive expression and subsequent loss of the mammary tumor virus. when the tumors were transplanted, levels of b particles first declined in ... | 1977 | 193632 |
| immunoelectron microscopic studies of antibodies in mouse sera directed against mouse mammary tumor virus. | indirect immunoferritin and fixed immunofluorescence tests were carried out on (a) sera of mice hyperimmunized with isologous mouse mammary tumor virus (mmtv) particles or isologous mmtv-producing mammary tumor cells grown in tissue culture and (b) sera of mammary tumor-bearing and tumor-free mice of several inbred strains. sera were tested against mmtv produced by c3h/hej/tex tissue culture cells (mmt-1). mammary tumor-bearing a/dm, c3h/hetex, and riii/dm mice and apparently tumor-free a/dm mic ... | 1977 | 193633 |
| organ distribution of exogenous murine mammary tumour virus as determined by bioassay. | | 1977 | 193692 |
| the role of t and suppressor cells in mtv-directed cellular immunity. | | 1977 | 193694 |
| [microsomal n-acetyl-glucosaminyl-transferases activity in balb/c mice infected with mouse mammary tumor virus (m-mtv) (author's transl)]. | | 1977 | 193720 |
| expression of thy 1 antigen in normal and neoplastic mammary cells of mice. | the expression of thy 1.2 (theta c3h) antigen was measured on the membranes of normal and neoplastic riii and c3h mammary cells. competitive inhibition assays revealed that the average membrane content of thy 1.2 in mammary tissues was about equal to that of lymph node cells. higher percentages of thy 1.2-positive cells than mammary tumor virus (mumtv)-positive cells were observed by immunofluorescence, which suggested that not all the thy 1.2-positive cells recovered from tumors were also mumtv ... | 1977 | 194040 |
| bioactivity of c3h and riii mammary tumor viruses in virgin female balb/c mice. | the bioactivities of c3h and riii mammary tumor virus (mumtv) in virgin female balb/c mice differed. the average number of mammary hyperplastic alveolar nodules per mouse after noduligenic tests was 20.1 in balb/cfc3h and 10.7 in balb/cfriii females. spontaneous mammary tumor incidence after 20 months of observation was 47.5% in balb/cfc3h and 14.6% in balb/cfriii females (p less than 0.01). the frequency of lung metastases in mammary tumor-bearing mice was 63.1% in balb/cfc3h and 16.6% in balb ... | 1977 | 194047 |
| role of h-2 histocompatibility on generation of cell-mediated cytotoxicity against virus-induced mammary tumors in c3h mice. | | 1977 | 194369 |
| migration inhibition and blastogenic reactions with viral and tumor antigens in transplantable mammary tumor systems. | | 1976 | 194519 |
| differences in mouse mammary tumor viruses. relationship to early and late occurring mammary tumors. | the murine model has been used extensively to study the various factors involved in the etiology of mammary carcinoma. inbred mouse strains have been classically categorized into (i) high incidence stains with tumors occurring relatively early in the life of the animal, or (ii) low or moderate incidence strains with tumors occurring later on in life. we have radioactively labeled the rna genome of the mouse mammary tumor virus (mmtv) from each of several mouse strains. we report here, using the ... | 1977 | 194675 |
| biology of breast preneoplasia. | the literature dealing with mammary cancer and the presence of the identifiable preneoplastic lesions in the mammary gland of several species has been reviewed. a discrete morphologically identifiable lesion with a high malignant potential is considered preneoplastic. lobuloalveolar lesions have been identified in the human mammary gland which fit most of the criteria for preneoplasia. the lesions, hyperplastic atypical lobules, are multicentric, have a high statistical association with breast c ... | 1977 | 194676 |
| natural immunity to murine mammary tumor viruses. | | 1977 | 194748 |
| immunogenicity and mumtv-like antigenicity of human breast cancer tissues. | | 1977 | 194749 |
| the etiology of breast cancer. | | 1976 | 194756 |
| immunologic studies of the low molecular weight dna binding protein of murine oncornaviruses. | a low molecular weight, highly basic dna-binding protein was purified from several oncornaviruses by the sequential procedures of gel filtration in guanidine-hydrochloride, deae-cellulose chromatography and affinity chromatography on single-stranded dna sepharose. the binding protein from rauscher and woolly monkey type-c viruses was the fastest migrating of the virion proteins in sds-polyacrylamide gels and thus is designated p10 according to previous convention although our estimates of molecu ... | 1977 | 194848 |
| effect of immune manipulation on natural immune responses to murine mammary tumor antigens. | the virus-host relationship in balb/cfc3h virgin female mice neonatally infected with an oncogenic virus (mammary tumor virus) (mumtv) was significantly altered by a brief immunosuppressive treatment during young adult life. natural immune responses were augmented and changed. the ability of lymphoid cells to attack target cells expressing mumtv-associated antigens was significantly increased, and a new component, a non-t-cell reactivity, was added to the t-cell reactivity found in normal female ... | 1977 | 195069 |
| electrophoretic analysis of the molecular weight of murine mammary tumor virus rna. | molecular weight determinations of native and subunit rnas of murine mammary tumor virus (mumtv), a type b oncornavirus, were performed by polyacrylamide gel electrophoresis and compared with molecular weights of well-characterized avian cellular rnas and tobacco mosaic virus rna. from extrapolations of semilog plots of the molecular weights of the standard rnas versus relative electrophoretic mobilities and ferguson plots, the subunit and native rnas of mumtv were found to possess molecular wei ... | 1977 | 195092 |
| the major polypeptides of the murine-mammary-tumor virus isolated by plant-lectin affinity chromatography. | solubilized polypeptides of the murine mammary tumor virus (mumt virus) were chromatographed on a column of immobilised concanavalin a. the unbound viral material was rechromatographed on phosphocellulose, resulting in the isolation of the major proteins with a molecular weight of 28000 (p28) and 12000 (p12) respectively. the adsorbed glycopolypeptides after elution with methyl alpha-d-mannopyranoside were subjected to gel filtration. the major glycoprotein with a molecular weight of 52000 (gp52 ... | 1977 | 195815 |
| biological activities of murine mammary tumour virus in vitro. increased macromolcular synetheses in mouse and hamster kidney cells; production of b- and c-particles in the mouse cells. | | 1977 | 195818 |
| simultaneous chemical induction of mtv and mlv in vitro. | | 1977 | 195819 |
| identification of the structural proteins of the murine mammary tumor virus that are serologically related to the antigens of intracytoplasmic type-a particles. | | 1977 | 196403 |
| murine mammary tumor virus protein vaccine: induction of antivirus immunity and inhibition or acceleration of growth of transplanted mammary tumors. | | 1977 | 197247 |
| glucocorticoid-stimulated accumulation of mouse mammary tumor virus rna: increased rate of synthesis of viral rna. | glucocorticoid hormones specifically increase the intracellular concentration of mouse mammary tumor virus (mmtv) rna in a cultured cell line from a gr mouse mammary carcinoma (gr) and in an mmtv-infected rat hepatoma cell line (m1.19). in contrast, these steroids have no effect on the concentration of mmtv rna in a lymphoma line, s49, from a balb/c mouse. using a molecular hybridization procedure to detect newly synthesized rna, we have directly measured the effect of dexamethasone, a synthetic ... | 1977 | 197523 |
| pseudotypes of vesicular stomatitis virus with envelope antigens provided by murine mammary tumor virus. | | 1977 | 197701 |
| regulation of mammary tumor virus production by prolactin in balb/cfc3h mouse normal mammary epithelial cells in vitro. | hormonal regulation of mammary tumor virus (mtv) production, has been analyzed with normal mammary epithelial cells from chronically infected balb/cfc3h mice. the effect of prolactin in terms of increased mtv production was not reproducibly observed in cells cultured in tissue culture dishes, whereas the cells grown on floating collagen gels consistently responded to prolactin in a dose-dependent manner. of the three media tested. dulbecco's modified eagle's medium was found to be the best in te ... | 1977 | 198118 |
| factors interfering with cellular immunological responses to the murine mammary tumor virus in tumor-bearing mice. | t-cell-enriched spleen cell fractions from balb/c mice bearing virally induced mammary tumors become unresponsive with progressive tumor growth in the lymphocyte blastogenesis test, using purified mammary tumor virus (mtv) as an antigen. reactivity can be restored by mild trypsinization or extensive washing of the cells. when reactive cells from mice immunized with mtv were incubated with the wash fluid of the initially unresponsive cells. mtv-specific blastogenesis was inhibited. washings of no ... | 1977 | 198383 |
| comparison of mouse mammary tumor virus-specific dna in inbred, wild and asian mice, and in tumors and normal organs from inbred mice. | | 1977 | 198552 |
| elution of tumor-directed antibody from kidneys of mammary tumor-bearing mice. | immunoglobulin-containing eluates, prepared from kidneys of paris riii mammary tumor-bearing mice approximately 10 months old, were incubated with frozen sections of mouse mammary tumor and normal lactating mammary gland and examined by immunofluorescence. the eluates diffusely strained the tumor but did not stain the nonneoplastic lactating mammary gland. mammary tumor and normal lactating mammary gland were both stained by an antiserum to mammary tumor virus (mumtv). the euglobulin portion of ... | 1977 | 198566 |
| transmission of mammary tumor virus in mouse strain dd: further support for the uniqueness of strain gr. | outcrosses between high tumor mouse strain dd and low-incidence strains balb/c and c57bl to produce reciprocal f1 susceptible offspring of both types resulted in high incidences of mammary tumors in (dd female x balb/c male)f1 and (dd female x c57bl male)f1 females but not in the reciprocal hybrids with dd as the male parent. the finding that transmission of murine mammary tumor virus (mumtv) in dd was through the milk, as most often observed in mouse strains, further supported the uniqueness of ... | 1977 | 198570 |
| the major structural proteins of murine mammary tumor virus: techniques for isolation. | | 1977 | 199080 |
| phospholipids of the milk-fat-globule membrane and the mouse mammary-tumour virus isolated from the milk of infected mice [proceedings]. | | 1977 | 199491 |
| paternal transmission of mammary tumor virus from dd/tbr strain of mice by crossing with balb/c or c57bl/6j strains. | | 1977 | 199517 |
| development of a congeneic line of the gr mouse strain without early mammary tumours. | based on the previous observation that mtv-2 controls the appearance of pregnancy-dependent mammary tumours and the early expression of mammary tumour virus (mtv)-antigens in the milk, an attempt was made to develop a congeneic gr line, without this locus, by introducing genetic material of the c57bl/10 strain. the cross-intercross system was used for this purpose. the mice of the even-numbered generations were selected for the absence of the mtv-2 locus with the early maammary tumour (emt) test ... | 1977 | 199548 |
| considerations on the preneoplastic lesions of the mammary gland. | the general characteristics of the preneoplastic lesions of the human mammary gland, as they are known through histologic description, are outlined, and data obtained from the experimental analysis of mammary gland preneoplasia in five areas of endeavor are discussed. results obtained with transplantation procedures and aimed at defining the growth potential of hyperplastic outgrowths are reported. information derived from the study of events able to induce benign hyperplastic outgrowths or thei ... | 1977 | 200144 |
| radioimmunoassay for glycoprotein gp47 of murine mammary tumor virus in organs and serum of mice and search for related antigens in human sera. | murine mammary tumor virus main glycoprotein (gp47), prepared by diethylaminoethyl cellulose and hydroxyapatite chromatography of detergent-mercaptoethanol-kci-disrupted virion, was used as labeled antigen in a highly specific and reproducible radioimmunoassay. seven other (glyco) proteins of the virus were antigenically distinct from gp47. serum and organs of unifected c57bl mice did not contain gp47, but sera of infected swiss and riii mice did contain the antigen. despite the high content in ... | 1977 | 200346 |
| long-term effects of prenatal and neonatal administration of 5beta-dihydrotestosterone on normal and neoplastic mammary development in mice. | the long-term effects on mammary glands of 5beta-dihydrotestosterone, considered to be biologically inactive, were studied in female shn mice with mammary tumor, virus. 5beta-dihydrotestosterone was adminstered to mothers for 4 days from day 12 to day 15 of pregnancy (prenatal treatment) and to pups for 5 days of postnatal life (neonatal treatment) at daily doses of 1 mg and 200 mug, respectively. neonatal treatment resulted in marked stimulation of spontaneous mammary tumorigenesis; all neonata ... | 1977 | 200350 |
| relative importance of genotype and type of mammary tumor virus on mammary tumorigenesis in mice. | | 1977 | 200431 |
| evidence for an influence of mammary tumour virus on prolactin secretion in the mouse. | | 1977 | 200691 |
| mammary tumors and mammary tumor virus expression in hybrid mice of strains c57bl and gr. | mammary tumorigenesis in genetic crosses between the high mammary tumor incidence gr and the low incidence c57bl mouse strains is highly correlated with murine mammary tumor virus expression in milk. although the f1 and first backcross females had a mammary tumor incidence which was consistent with a single dominant gene segregation, the tumor incidence in the critical second backcross segregants disproved the single gene hypothesis. genetic factors were clearly involved in regulation of virus e ... | 1977 | 200697 |
| [increase in the frequency of occurrence of mammary neoplasms in rats infected with mouse bittner virus]. | | 1977 | 201438 |
| [studies on reproduction and pregnancy--dependent mammary tumors of gr/a mice (author's transl)]. | | 1977 | 201476 |
| immunopathology of breast cancer. | | 1977 | 201905 |
| cellular immune responsiveness of breast cancer patients to parsi human milk particles & mammary tumor virus: lymphocyte blastogenesis. | | 1977 | 202562 |
| oncogenicity of murine mammary tumor virus produced in tissue culture: brief communication. | murine mammary tumor virus (mumtv), produced from a glucocorticoid-stimulated c3h mouse mammary adenocarcinoma cell line, was free of murine leukemia virus and oncogenic for weanling balb/c mice. adenocarcinomas were induced by mumtv as early as 136 days post inoculation and with as low as 5 x 10(3) virus particles/mouse. tumor incidence did not correlate directly with virus dose; rather, it was low at higher mumtv concentrations (1.2 x 10(8) particles/mouse), reached and optimum at 1.3 x 10(5) ... | 1978 | 202721 |
| synthesis and processing of precursor polypeptides to murine mammary tumor virus structural proteins. | biosynthesis of murine mammary tumor virus (mumtv) proteins was studied in the chronically mumtv-infected epithelial cell line mumt-73 by using monospecific antisera to the major mumtv core protein p27 and the major envelope glycoprotein gp47. in pulse-labeling experiments using [35s]methionine, monospecific antisera to p27 precipitated a 75,000-molecular-weight protein as the major intracellular component. analysis of the same cellular extracts with monospecific antisera to gp47 revealed that t ... | 1978 | 202748 |
| quantitative determination of mammary tumor virus in individual samples of mouse milk. | the possibility to determine quantitatively the intact mammary tumor virus (mtv) in milk of mice carrying milk-transmitted mtv has been assayed by a method that allows direct comparison between individual milk samples. the method is based on (a) the measure of light scattering of partially purified mtv preparations, (b) the use of milk from genetically identical mtv free mice as blank and (c) the quantitative reference to the total protein content of whole milk. the sensitivity, specificity and ... | 1977 | 203070 |
| quantitative comparison of milk-released c3h and riii mammary tumor viruses in infected balb/c hosts. | the c3h and riii mammary tumor viruses (mtv) carried by balb/cfc3h and balb/cfriii breeding females have been quantified and compared in milk samples, after partial purification with a sucrose density gradient. the samples were collected at identical times during the first 3 lactation periods from individual mice (6 per strain), standardized for age at delivery and size of litter. milk samples from 6 mtv negative balb/c controls have also been analyzed. data for comparison are expressed in optic ... | 1977 | 203071 |
| hydrophobic interaction of retroviral dna polymerases with alkyl-agarose matrices. | | 1978 | 203279 |
| in situ embedding method of cells grown in beem capsules for immunoelectron microscopic studies of oncornaviruses. | a technique of in situ embedding of cells grown in beem capsules has been devised for immunoelectron microscopic studies of oncornaviruses. as compared to other immunoelectron microscopic procedures, this technique is less time and reagent-consuming. the quality and specificity of this method were tested on well-characterized mouse mammary tumor virus (type b virus) and murine sarcoma virus (type c virus particles). this method gave good results in labeling of the virus particles with ferritin o ... | 1978 | 203626 |
| suppressor cells in mice with murine mammary tumor virus-induced mammary tumors. i. inhibition of mitogen-induced lymphocyte stimulation. | suppressor cell activity was present in the glass-adherent fraction of spleen cells from c3h mice bearing murine mammary tumor virus-induced mammary tumors. these cells effectively suppressed the blastogenic response of syngeneic normal lymphocytes to concanavalin a (con a). suppression by the spleen cells from mammary tumor-bearing mice was not dependent on dna synthesis. removal of the suppressor cells from spleen cell suspensions of tumor-bearing mice was not dependent on dna synthesis. remov ... | 1978 | 203703 |
| in vitro transcription of mouse mammary tumor virus rna into dna. | the in vitro transcription of the b-type mouse mammary tumor virus rna into dna is similar to that obtained with certain c-type viruses: synthesis can be initiated on an rna primer molecule approximately the size of a trna, and a unique linkage (ra-dc) exists between this primer and the dna product. | 1978 | 203724 |
| interaction between viral and genetic factors in murine mammary cancer. | | 1978 | 204164 |
| characterisation of mammary tumour virus of strain icrc mouse. | | 1978 | 204490 |
| in vitro studies on splenocyte cytotoxicity against syngeneic mammary carcinomas of mice. 1. reactivity of splenocytes from mice bearing mammary tumors against the corresponding and against other neoplasms. | | 1978 | 204609 |
| alteration of sodium transport in mouse mammary epithelium associated with neoplastic transformation. | using electrophysiological techniques we have examined the apical membrane ionic permeabilities of primary cell cultures of the mouse mammary gland in the midpregnant, preneoplastic, and neoplastic states. membrane na+ permeability changed with tumorigenesis, whereas k+ and cl- permeabilities were unaltered. with tracer flux techniques the unidirectional efflux rate constant of 22na was found to be greater in tumor cells than it is in normal cells. this increase in 22na efflux was eliminated by ... | 1978 | 205364 |
| immunization of mice against murine mammary tumor virus infection and mammary tumor development. | formalin-inactivated whole murine mammary tumor virus (mumtv), vumtv membranes, the acid-soluble component of mumtv, and purified mumtv glycoprotein with a molecular weight of 55,000 (gp55; also designated as gp52) were used as vaccines in an attempt to identify the mumtv antigen(s) that can protect mice from exogenous mumtv infection and subsequent tumor development. formalin-inactivated whole mumtv, mumtv membranes, and purified mumtv gp55 were effective immunogens, whereas the acid-soluble co ... | 1978 | 205366 |
| the use of molecular hybridization to track the mode of transmission and distribution of murine mammary tumor viruses: a model for etiologic studies of human breast cancer. | | 1978 | 205905 |
| [carcinogenesis due to oncorna virus type c: biological, immunological and genetic aspects]. | | 1977 | 205925 |
| serological and biochemical characterization of the mouse mammary tumor virus with localization of p10. | | 1978 | 205999 |
| isolation of separate precursor polypeptides for the mouse mammary tumor virus glycoproteins and nonglycoproteins. | | 1978 | 206000 |
| [glucocorticoid receptors in mammary tumors in ps and c3h mouse strains (author's transl)]. | specific binding of glucocorticoids was characterized in mammary tumors and cells derived from c3h and ps mice. the binding protein satisfied all the general criteria of hormone receptors namely high affinity and binding specificity for glucocorticoids (kd congruent to 2.5 nm) for 3h dexamethasone and limited number of binding sites (congruent to 150 femtomoles/mg protein). the hormonal regulation of these receptors will be reported. the production of viral particles of type a and b was increase ... | 1977 | 206179 |
| synthesis of mouse mammary tumor virus ribonucleic acid in isolated nuclei from cultured mammary tumor cells. | glucocorticoid hormone treatment of gr cells, a cultured line derived from mouse mammary tumor tissue, selectively stimulates the rate of transcription of integrated proviral genes specifying mammary tumor virus (mtv). we have incubated isolated nuclei from these cells under conditions in which all three endogenous rna polymerases appear to be active. rna synthesized in vitro is distinguished from preexisting nuclear rna by labeling the in vitro products with [3h]ctp, and the level of mtv rna sy ... | 1978 | 206279 |
| radioimmunoassay for protein p28 of murine mammary tumor virus in organs and serum of mice and search for related antigens in human sera and breast cancer extracts. | the main protein of the core of murine mammary tumor virus, with a molecular weight of 28,000 (p28), was solubilized by deoxycholate treatment of the virus and purified by ultrogel aca-54 filtration and hydroxyapatite chromatography. this protein was used as labeled antigen in a highly specific and reproducible radioimmunoassay. organ extracts of uninfected c57bl mice did not contain p28, but organ extracts of infected riii mice did contain the antigen. despite the high content in the mammary gl ... | 1978 | 206358 |
| leukocyte migration inhibition and lymphocyte blastogenesis responses in breast carcinoma patients to mouse mammary tumor virus and to virion gp52 antigen and rauscher murine leukemia virus-kirsten sarcoma virus gp69/71 antigen. | | 1978 | 206704 |
| effect of a mouse mammary tumor virus-derived protein vaccine on primary tumor development in mice. | the vaccines used in this study were derived from purified murine mammary tumor virus (mumtv) preparations. approximately 60% of the protein fractions consisted of the major viral membrane glycoprotein gp52. inoculation sc of 10 microgram mumtv-s-derived vaccine significantly delayed the appearance of primary mammary tumors in gr and balb/cfc3h mice (strains with high incidences of mammary cancer); in balb/c and c3hf mice, which have a moderate tumor incidence at an advanced age, this treatment ... | 1978 | 206711 |
| genetic control of immune responses to moloney leukemia virus in rats. | when bn and lew rats were immunized with untreated or with inactivated moloney murine leukemia virus (m-mulv), bn rats produced high antibody responses to the p15, p30, and gp70 antigens of the virus, whereas lew rats were low responders to these antigens. bn rats also exhibited a high response and lew rats a low response when the two strains were immunized with purified p30. studies of (lew x bn)f1 and backcross rats suggested that factors associated with agb exerted major influences on respons ... | 1978 | 206712 |
| production of unintegrated mouse mammary tumor virus dna in infected rat hepatoma cells is a secondary action of dexamethasone. | dexamethasone, a synthetic glucocorticoid, selectively increased the rate of synthesis of mouse mammary tumor virus (mtv) rna in clonal isolates of chronically infected rat hepatoma tissue culture cells. this hormonal effect occurred extremely rapidly and appeared to be mediated directly by the glucocorticoid-specific receptor protein. in addition to the viral rna, unintegrated mtv dna was also detected in these cells. several lines of evidence are consistent with the idea that the unintegrated ... | 1978 | 206732 |
| detection of viral proteins in mouse mammary tumors by immunoperoxidase staining of paraffin sections. | an indirect immunoperoxidase method is described, which can readily detect viral antigens in paraffin sections of primary, transplanted, and metastatic mammary tumors of mice. in addition to having the obvious advantage of not being limited to fresh specimens, immunoperoxidase staining of paraffin sections proved to be superior in many respects when compared with immunofluorescence and frozen sections. immunoperoxidase staining of paraffin sections is permanent and provides the kind of histologi ... | 1978 | 206904 |
| detection in human breast carcinomas of an antigen immunologically related to a group-specific antigen of mouse mammary tumor virus. | an antigen immunologically related to a group-specific antigen (gp52, a 52,000-dalton glycoprotein) of the mouse mammary tumor virus has been identified in paraffin sections of human breast cancers by means of the indirect immunoperoxidase technique. the specificity of the reaction with antibody against mouse mammary tumor virus was examined by absorption of the igg with the following: (a) purified gp52; (b) a number of virus preparations (mouse mammary tumor virus, rauscher leukemia virus, simi ... | 1978 | 206905 |
| comparative effects of diphenylglioxal and its superoxide on experimental tumors. | | 1977 | 207230 |
| cellular immunity to autologous breast cancer and riii-murine mammary tumor virus preparations. | | 1978 | 207421 |
| detection of inapparent nodule-transformed cells in the mammary gland tissues of virgin female balb/cfc3h mice. | inapparent nodule-transformed cells were recovered from morphologically normal mammary tissue from virgin female balb/cfc3h/crgl (mouse mammary tumor-positive) mice before the appearance of hyperplastic alveolar nodules (han) or tumors, by means of the cell dissociation technique. mammary tissues were dissociated by means of collagenase (0.1%), hyaluronidase (0.1%), and pronase (1.25%). aliquots of 10(5) viable cells in 0.01 ml medium were injected into the gland-free mammary fat pads of 3-week- ... | 1978 | 207423 |
| hormonal control of mrna synthesis studied by nucleic acid hybridization. | the regulation of protein synthesis by steroids is thought to be due to hormonal effects primarily on mrna concentration. experimental evidence to support this conclusion has come largely from the use of dna probes complementary (cdna) to mrna molecules or by translation of the mrna in vitro. in this review the experimental procedures involved and the application to hormone action of cdna hybridization will be reviewed. (1) mrna concentrations can be assayed in tissue rna samples by hybridizatio ... | 1978 | 207603 |
| induction of mammary tumors in c57bl/haag female mice by a low oncogenic mammary tumor virus. | mammary tumor virus (mumtv)-negative mouse substrain c57bl/haag foster-nursed by strain dd becomes a line with a moderately high incidence of mammary tumors occurring in the females at a late age. electron microscopy revealed b particles in the tumors, providing additional evidence for the propagation and transmission of dd-mumtv in c57bl/haagfdd. since both albino strains dd and riii originated in europe, develop pregnancy-dependent mammary tumors, carry black and agouti genes (cc, bb, aa), and ... | 1978 | 207649 |
| mouse mammary tumor virus genes: regulation of expression by glucocorticoids and structural analysis with restriction endonucleases. | the production of mouse mammary tumor virus (mtv) is stimulated by treatment of mammary carcinoma cells with glucocorticoid hormones (e.g., dexamethasone). the reaction appears to be a primary and receptor-mediated phenomenon in which the rate of synthesis of mtv rna is selectively increased; the maximally induced rate is reached within 15 min after addition of the hormone. production of mtv rna is also stimulated by dexamethasone in some but not all clonal isolates of nonmurine cells infected b ... | 1978 | 207882 |
| structural components of mouse mammary tumor virus. ii. isolation and purification of virion polypeptides. | mouse mammary tumor virus (mmtv) glycoproteins and nonglycosylated polypeptides were purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. primary amino groups were labeled with fluorescamine to enable visualization of mmtv polypeptides in the gels. protein bands were sliced from the gels and eluted with 90 to 95% recovery. eight mmtv polypeptides, including three of the major viral components as well as five minor proteins, were routinely obtained. double diffusion ... | 1978 | 207897 |
| the relative hydrophobicity of oncornaviral structural proteins. | | 1978 | 208256 |
| coexistence of the mouse mammary tumor virus (mmtv) major glycoprotein and natural antibodies to mmtv in sera of mammary tumor-bearing mice. | | 1978 | 208275 |
| changes in mumtv dna and rna levels in balb/c mammary epithelial cells during malignant transformation by exogenous mutv and by hormones. | | 1978 | 208280 |