| evaluation of dna fragments covering the entire genome of a monopartite begomovirus for induction of viral resistance in transgenic plants via gene silencing. | tomato-infecting begomoviruses, a member of whitefly-transmitted geminivirus, cause the most devastating virus disease complex of cultivated tomato crops in the tropical and subtropical regions. numerous strategies have been used to engineer crops for their resistance to geminiviruses. however, nearly all have concentrated on engineering the replication-associated gene (rep), but not on a comprehensive evaluation of the entire virus genome. in this study, tomato leaf curl taiwan virus (tolctwv), ... | 2011 | 21597979 |
| infection with a plant virus modifies vector feeding behavior. | vector infection by some animal-infecting parasites results in altered feeding that enhances transmission. modification of vector behavior is of broad adaptive significance, as parasite fitness relies on passage to a new host, and vector feeding is nearly always essential for transmission. although several plant viruses infect their insect vectors, we have shown that vector infection by a plant virus alters feeding behavior. here we show that infection with tomato spotted wilt virus (tswv), type ... | 2011 | 21606372 |
| high incidence of tobacco streak virus in the tobacco fields of iran. | currently the northern provinces of iran (mazandaran, golestan and gilan) are the main tobacco growing regions in the country and this crop has an special importance in the national economy. three tobacco types including flue-cured (mainly coker 347 and some virginia e1), burley (burley 21) and oriental (basma 178-2) are presently grown in these regions. epidemics of viral diseases have occurred during the recent years in many tobacco fields in these areas. the quality of tobacco products which ... | 2006 | 17390881 |
| detection, discrimination and absolute quantitation of tomato spotted wilt virus isolates using real time rt-pcr with taqman(®)mgb probes. | a quantitative real-time reverse transcription-polymerase chain reaction (rt-qpcr) procedure using a general primer set and three taqman(®)mgb probes was developed for general and genotype-specific detection and quantitation of the genomic m segment of tomato spotted wilt virus (tswv). standard curves using rna transcripts homologous to the three probes allowed reproducible quantitative assays with a wide dynamic range (10(3)-10(10) tswv m segment rna copies/ng of total rna) and high sensitivity ... | 2011 | 21635923 |
| complete genome sequence of a tomato spotted wilt virus isolate from china and comparison to other tswv isolates of different geographic origin. | tomato spotted wilt virus (tswv) is well established in most countries worldwide, while it is rarely reported in china. in this report, we have determined the complete nucleotide sequence of a tswv isolate named tswv-yn infecting tomato in yunnan province in southwestern china. the tripartite genome of tswv-yn was found to consist of l, m and s rnas of 8910, 4773 and 2970 nt, respectively. the complete genome sequence and the sequence of each genomic region of tswv-yn from china were compared to ... | 2011 | 21805095 |
| occurrence of viruses infecting pea in iran. | a survey was conducted to determine the incidence of alfalfa mosaic virus (amv), bean yellow mosaic virus (bymv), broad bean wilt virus-1 (bbwv), pea leafroll virus (plrv), pea enation mosaic virus (pemv), pea seed borne mosaic virus (psbmv), potato virus x(pvx), tomato mosaic virus (tomv), tomato spotted wilt virus (tswv) on pea (pisum sativum) in iran. a total of 1276 random and 684 symptomatic pea samples were collected during the spring and summer of 2002-2004 in tehran province of iran, whe ... | 2006 | 17390891 |
| arbuscular mycorrhizal symbiosis limits foliar transcriptional responses to viral infection and favors long-term virus accumulation. | tomato (solanum lycopersicum) can establish symbiotic interactions with arbuscular mycorrhizal (am) fungi, and can be infected by several pathogenic viruses. here, we investigated the impact of mycorrhization by the fungus glomus mosseae on the tomato spotted wilt virus (tswv) infection of tomato plants by transcriptomic and hormones level analyses. in tswv-infected mycorrhizal plants, the am fungus root colonization limited virus-induced changes in gene expression in the aerial parts. the virus ... | 2011 | 21899386 |
| evidence that the nonstructural protein of tomato spotted wilt virus is the avirulence determinant in the interaction with resistant pepper carrying the tsw gene. | all known pepper cultivars resistant to tomato spotted wilt virus (tswv) possess a single dominant resistance gene, tsw. recently, naturally occurring resistance-breaking (rb) tswv strains have been identified, causing major concerns. we used a collection of such strains to identify the specific genetic determinant that allows the virus to overcome the tsw gene in capsicum spp. a reverse genetic approach is still not feasible for tswv; therefore, we analyzed reassortants between wild-type (wt) a ... | 2007 | 17506332 |
| Antagonistic plant defense system regulated by phytohormones assists interactions among vector insect, thrips, and a tospovirus. | The western flower thrips (Frankliniella occidentalis) is a polyphagous herbivore that causes serious damage to many agricultural plants. In addition to causing feeding damage, it is also a vector insect that transmits tospoviruses such as Tomato spotted wilt virus (TSWV). We previously reported that thrips feeding on plants induces a jasmonate (JA)-regulated plant defense, which negatively affects both the performance and preference (i.e., host plant attractiveness) of the thrips. The antagonis ... | 2011 | 22180600 |
| modified expression of alternative oxidase in transgenic tomato and petunia affects the level of tomato spotted wilt virus resistance. | abstract: | 2011 | 22014312 |
| sequence determination and analysis of the nss genes of two tospoviruses. | the tospoviruses groundnut ringspot virus (grsv) and zucchini lethal chlorosis virus (zlcv) cause severe losses in many crops, especially in solanaceous and cucurbit species. in this study, the non-structural nss gene and the 5'utrs of these two biologically distinct tospoviruses were cloned and sequenced. the nss sequence of grsv and zlcv were both 1,404 nucleotides long. pairwise comparison showed that the nss amino acid sequence of grsv shared 69.6% identity with that of zlcv and 75.9% identi ... | 2011 | 22187101 |
| the nucleoprotein of tomato spotted wilt virus as protein tag for easy purification and enhanced production of recombinant proteins in plants. | upon infection, tomato spotted wilt virus (tswv) forms ribonucleoprotein particles (rnps) that consist of nucleoprotein (n) and viral rna. these aggregates result from the homopolymerization of the n protein, and are highly stable in plant cells. these properties feature the n protein as a potentially useful protein fusion partner. to evaluate this potential, the n protein was fused to the aequorea victoria green fluorescent protein (gfp), either at the amino or carboxy terminus, and expressed i ... | 2007 | 17532227 |
| The nucleocapsid protein of an enveloped plant virus, Tomato spotted wilt virus, facilitates long-distance movement of Tobacco mosaic virus hybrids. | To investigate the potential role(s) of the nucleocapsid (N) protein of Tomato spotted wilt virus (TSWV), the open reading frame for the N protein was expressed from a Tobacco mosaic virus (TMV)-based vector encoding only the TMV replicase proteins. In the absence of other TSWV-encoded proteins, the transiently expressed N protein facilitated long-distance movement of the TMV-based hybrids in transgenic Nicotiana benthamiana [NB-MP(+)] expressing movement protein of TMV, thus providing the funct ... | 2011 | 22020361 |
| an integrated genetic linkage map of cultivated peanut (arachis hypogaea l.) constructed from two ril populations. | construction and improvement of a genetic map for peanut (arachis hypogaea l.) continues to be an important task in order to facilitate quantitative trait locus (qtl) analysis and the development of tools for marker-assisted breeding. the objective of this study was to develop a comparative integrated map from two cultivated × cultivated recombinant inbred line (ril) mapping populations and to apply in mapping tomato spotted wilt virus (tswv) resistance trait in peanut. a total of 4,576 simple s ... | 2011 | 22072100 |
| biological and molecular analyses of the acibenzolar-s-methyl-induced systemic acquired resistance in flue-cured tobacco against tomato spotted wilt virus. | tomato spotted wilt virus (tswv) is an economically important virus of flue-cured tobacco. activation of systemic acquired resistance (sar) by acibenzolar-s-methyl (asm) in flue-cured tobacco was studied under greenhouse conditions by challenge inoculation with a severe isolate of tswv. asm restricted virus replication and movement, and as a result reduced systemic infection. activation of resistance was observed within 2 days after treatment with asm and a high level of resistance was observed ... | 2008 | 18943196 |
| salicylic acid is involved in the basal resistance of tomato plants to citrus exocortis viroid and tomato spotted wilt virus. | tomato plants expressing the nahg transgene, which prevents accumulation of endogenous salicylic acid (sa), were used to study the importance of the sa signalling pathway in basal defence against citrus exocortis viroid (cevd) or tomato spotted wilt virus (tswv). the lack of sa accumulation in the cevd- or tswv-infected nahg tomato plants led to an early and dramatic disease phenotype, as compared to that observed in the corresponding parental money maker. addition of acibenzolar-s-methyl, a ben ... | 2016 | 27893781 |
| dynamics of tomato spotted wilt virus replication in the alimentary canal of two thrips species. | abstract transmission of tomato spotted wilt virus (tswv) is dependent on virus uptake in the midgut prior to virus movement to the salivary glands. replication of tswv in the alimentary canal of tobacco thrips (tt, frankliniella fusca) and western flower thrips (wft, f. occidentalis) was investigated by immunolocalization of the nonstructural protein (nss) encoded by the small rna of tswv and fluorescence microscopy. analysis of cohorts during development from larva to adults following virus ac ... | 2002 | 18943268 |
| acquisition of tomato spotted wilt virus by adults of two thrips species. | abstract only larval thrips that acquire tomato spotted wilt virus (tswv), or adults derived from such larvae, transmit the virus. nonviruliferous adults can ingest virus particles while feeding on tswv-infected plants, but such adult thrips have not been shown to transmit tswv. immunofluorescence microscopy was used to show that thrips 1, 5, 10, and 20 days after adult emergence (dae) fed on tswv-infected plants acquired tswv with virus replication and accumulation occurring in both epithelial ... | 2004 | 18944108 |
| proteomic analysis of frankliniella occidentalis and differentially expressed proteins in response to tomato spotted wilt virus infection. | tomato spotted wilt virus (tswv) is transmitted by frankliniella occidentalis in a persistent propagative manner. despite the extensive replication of tswv in midgut and salivary glands, there is little to no pathogenic effect on f. occidentalis. we hypothesize that the first-instar larva (l1) of f. occidentalis mounts a response to tswv that protects it from pathogenic effects caused by virus infection and replication in various insect tissues. a partial thrips transcriptome was generated using ... | 2012 | 22696645 |
| the tomato spotted wilt virus genome is processed differentially in its plant host arachis hypogaea and its thrips vector frankliniella fusca. | thrips-transmitted tospoviruses are economically important viruses affecting a wide range of field and horticultural crops worldwide. tomato spotted wilt virus (tswv) is the type member of the tospovirus genus with a broad host range of more than 900 plant species. interactions between these viruses and their plant hosts and insect vectors via rnai pathways are likely a key determinant of pathogenicity. the current investigation, for the first time, compares biogenesis of small rnas between the ... | 2016 | 27656190 |
| non-structural proteins of arthropod-borne bunyaviruses: roles and functions. | viruses within the bunyaviridae family are tri-segmented, negative-stranded rna viruses. the family includes several emerging and re-emerging viruses of humans, animals and plants, such as rift valley fever virus, crimean-congo hemorrhagic fever virus, la crosse virus, schmallenberg virus and tomato spotted wilt virus. many bunyaviruses are arthropod-borne, so-called arboviruses. depending on the genus, bunyaviruses encode, in addition to the rna-dependent rna polymerase and the different struct ... | 2013 | 24100888 |
| tsw gene-based resistance is triggered by a functional rna silencing suppressor protein of the tomato spotted wilt virus. | as a result of contradictory reports, the avirulence (avr) determinant that triggers tsw gene-based resistance in capsicum annuum against the tomato spotted wilt virus (tswv) is still unresolved. here, the n and nss genes of resistance-inducing (ri) and resistance-breaking (rb) isolates were cloned and transiently expressed in resistant capsicum plants to determine the identity of the avr protein. it was shown that the nss(ri) protein triggered a hypersensitive response (hr) in tsw-containing ca ... | 2013 | 23360130 |
| the functional analysis of distinct tospovirus movement proteins (nsm) reveals different capabilities in tubule formation, cell-to-cell and systemic virus movement among the tospovirus species. | the lack of infectious tospovirus clones to address reverse genetic experiments has compromised the functional analysis of viral proteins. in the present study we have performed a functional analysis of the movement proteins (nsm) of four tospovirus species bean necrotic mosaic virus (benmv), chrysanthemum stem necrosis virus (csnv), tomato chlorotic spot virus (tcsv) and tomato spotted wilt virus (tswv), which differ biologically and molecularly, by using the alfalfa mosaic virus (amv) model sy ... | 2017 | 27697453 |
| the movement protein (nsm) of tomato spotted wilt virus is the avirulence determinant in the tomato sw-5 gene-based resistance. | the avirulence determinant triggering the resistance conferred by the tomato gene sw-5 against tomato spotted wilt virus (tswv) is still unresolved. sequence comparison showed two substitutions (c118y and t120n) in the movement protein nsm present only in tswv resistance-breaking (rb) isolates. in this work, transient expression of nsm of three tswv isolates [rb1 (t120n), rb2 (c118y) and non-resistance-breaking (nrb)] in nicotiana benthamiana expressing sw-5 showed a hypersensitive response (hr) ... | 2014 | 24690181 |
| resistance breaking tomato spotted wilt virus isolates on resistant pepper varieties in italy. | in spring 2012, resistance breaking (rb) isolates of tomato spotted wilt virus (tswv) that overcome the resistance conferred by the tsw gene in different pepper hybrids have been recovered in different locations in southern italy (campania and apulia regions) in protected cultivation, about one month after transplant. the percentage of symptomatic plants was 5-10% and, only in particular cases of advanced stage of cultivation, it reached 30-50% at the end of cycle. all tswv isolates induced simi ... | 2013 | 25151838 |
| thrips resistance in pepper and its consequences for the acquisition and inoculation of tomato spotted wilt virus by the western flower thrips. | abstract different levels of thrips resistance were found in seven capsicum accessions. based on the level of feeding damage, host preference, and host suitability for reproduction, a thrips susceptible and a resistant accession were selected to study their performance as tomato spotted wilt virus (tswv) sources and targets during thrips-mediated virus transmission. vector resistance did not affect the virus acquisition efficiency in a broad range of acquisition access periods. inoculation effic ... | 2003 | 18944162 |
| genome-wide identification and expression profiling of tomato hsp20 gene family in response to biotic and abiotic stresses. | the hsp20 genes are involved in the response of plants to environment stresses including heat shock and also play a vital role in plant growth and development. they represent the most abundant small heat shock proteins (shsps) in plants, but little is known about this family in tomato (solanum lycopersicum), an important vegetable crop in the world. here, we characterized heat shock protein 20 (slhsp20) gene family in tomato through integration of gene structure, chromosome location, phylogeneti ... | 2016 | 27582749 |
| simultaneous detection of tomato spotted wilt virus, dahlia mosaic virus and chrysanthemum stunt viroid by multiplex rt-pcr in dahlias and their distribution in japanese dahlias. | tomato spotted wilt virus (tswv), dahlia mosaic virus (dmv) and chrysanthemum stunt viroid (csvd) are economically important viruses and viroid that infect cultivated dahlias. prior to this investigation, no multiplex rt-pcr assay for the detection of dahlia virus and viroid infections existed. in this study, we report the development of a multiplex rt-pcr that simultaneously detects tswv, dmv and csvd infections in dahlias. in addition, a simple rt-pcr method that does not require rna extractio ... | 2015 | 25976592 |
| development of reverse transcription thermostable helicase-dependent dna amplification for the detection of tomato spotted wilt virus. | a protocol for the reverse transcription-helicase-dependent amplification (rt-hda) of isothermal dna was developed for the detection of tomato spotted wilt virus (tswv). specific primers, which were based on the highly conserved region of the n gene sequence in tswv, were used for the amplification of virus's rna. the lod of rt-hda, reverse transcriptase-loop-mediated isothermal amplification (rt-lamp), and reverse transcriptase-polymerase chain reaction (rt-pcr) assays were conducted using 10-f ... | 2016 | 27538747 |
| comparative analysis of chrysanthemum transcriptome in response to three rna viruses: cucumber mosaic virus, tomato spotted wilt virus and potato virus x. | the chrysanthemum is one of popular flowers in the world and a host for several viruses. so far, molecular interaction studies between the chrysanthemum and viruses are limited. in this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including cucumber mosaic virus (cmv), tomato spotted wilt virus (tswv) and potato virus x (pvx). a chrysanthemum 135k microarray derived from expressed sequence tags was successfully applied for the expression ... | 2015 | 25904110 |
| coat protein mutations in an attenuated cucumber mosaic virus encoding mutant 2b protein that lacks rna silencing suppressor activity induces chlorosis with photosynthesis gene repression and chloroplast abnormalities in infected tobacco plants. | in tobacco plants, the cucumber mosaic virus (cmv) pepo strain induces mosaic symptoms, including pale green chlorosis and malformed tissues. here, we characterized the involvement of 2b protein and coat protein (cp) in the development of mosaic symptoms. a 2b mutant (r46c) that lacks viral suppressor of rna silencing (vsr) activity showed an asymptomatic phenotype with low levels of virus accumulation. tomato spotted wilt virus nss protein did not complement the virulence of the r46c, although ... | 2014 | 24889248 |
| simultaneous detection of the seven main tomato-infecting rna viruses by two multiplex reverse transcription polymerase chain reactions. | cucumber mosaic virus, tomato spotted wilt virus, tomato mosaic virus, tomato chlorosis virus, pepino mosaic virus, torrado tomato virus and tomato infectious chlorosis virus cause serious damage and significant economic losses in tomato crops worldwide. the early detection of these pathogens is essential for preventing the viruses from spreading and improving their control. in this study, a procedure based on two multiplex rt-pcrs was developed for the sensitive and reliable detection of these ... | 2012 | 22939978 |
| viruses of pepper crops in the mediterranean basin: a remarkable stasis. | compared to other vegetable crops, the major viral constraints affecting pepper crops in the mediterranean basin have been remarkably stable for the past 20 years. among these viruses, the most prevalent ones are the seed-transmitted tobamoviruses; the aphid-transmitted potato virus y and tobacco etch virus of the genus potyvirus, and cucumber mosaic virus member of the genus cucumovirus; and thrips-transmitted tospoviruses. the last major viral emergence concerns the tospovirus tomato spotted w ... | 2012 | 22682167 |
| isolation and analysis of small rnas from virus-infected plants. | in this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small rnas (vsrnas). these methods have been successfully used for four plant viruses: cucumber mosaic virus (cmv), including the cmv y-satellite, turnip mosaic virus (tumv), potato leaf roll virus (plrv), and tomato spotted wilt virus (tswv) from inoculated arabidopsis thaliana plants (fusaro et al. embo rep 7:1168-1175, 2006; curtin et al. febs lett 582:2753-2760, 2008). the pro ... | 2012 | 22678580 |
| homology modeling and molecular dynamics provide structural insights into tospovirus nucleoprotein. | tospovirus is a plant-infecting genus within the family bunyaviridae, which also includes four animal-infecting genera: hantavirus, nairovirus, phlebovirus and orthobunyavirus. compared to these members, the structures of tospovirus proteins still are poorly understood. despite multiple studies have attempted to identify candidate n protein regions involved in rna binding and protein multimerization for tospovirus using yeast two-hybrid systems (y2hs) and site-directed mutagenesis, the tospoviru ... | 2016 | 28105914 |
| development of a microarray for simultaneous detection and differentiation of different tospoviruses that are serologically related to tomato spotted wilt virus. | tospoviruses, the plant-infecting genus in the family bunyaviridae, are thrips borne and cause severe agricultural losses worldwide. based on the serological relationships of the structural nucleocapsid protein (np), the current tospoviruses are divided into six serogroups. the use of np-antisera is convenient for virus detection, but it is insufficient to identify virus species grouped in a serogroup due to the serological cross-reaction. alternatively, virus species can be identified by the n ... | 2017 | 28081705 |
| analysis of tospovirus nss proteins in suppression of systemic silencing. | rna silencing is a sequence-specific gene regulation mechanism that in plants also acts antiviral. in order to counteract antiviral rna silencing, viruses have evolved rna silencing suppressors (rss). in the case of tospoviruses, the non-structural nss protein has been identified as the rss. although the tomato spotted wilt virus (tswv) tospovirus nss protein has been shown to exhibit affinity to long and small dsrna molecules, its ability to suppress the non-cell autonomous part of rna silencin ... | 2015 | 26275304 |
| genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus tospovirus. | chrysanthemum stem necrosis virus (csnv) is a member of a tentative tospovirus species. in this study, the complete genomic sequence of the japanese csnv isolate tcch07a was determined. the l rna is 8960 nt long and encodes the 331.0-kda rna-dependent rna polymerase. the m rna is 4828 nt long and encodes the 34.1-kda movement protein (nsm) and the 127.7-kda glycoprotein precursor (gn/gc). the s rna is 2949 nt long and encodes the 52.4-kda silencing suppressor protein (nss) and the 29.3-kda nucle ... | 2015 | 25427981 |
| emergence of groundnut ringspot virus and tomato chlorotic spot virus in vegetables in florida and the southeastern united states. | groundnut ringspot virus (grsv) and tomato chlorotic spot virus (tcsv) are two emerging tospoviruses in florida. in a survey of the southeastern united states, grsv and tcsv were frequently detected in solanaceous crops and weeds with tospovirus-like symptoms in south florida, and occurred sympatrically with tomato spotted wilt virus (tswv) in tomato and pepper in south florida. tswv was the only tospovirus detected in other survey locations, with the exceptions of grsv from tomato (solanum lyco ... | 2015 | 25317844 |
| analysis of tomato spotted wilt virus nss protein indicates the importance of the n-terminal domain for avirulence and rna silencing suppression. | recently, tomato spotted wilt virus (tswv) nonstructural protein nss has been identified unambiguously as an avirulence (avr) determinant for tomato spotted wilt (tsw)-based resistance. the observation that nss from two natural resistance-breaking isolates had lost rna silencing suppressor (rss) activity and avr suggested a link between the two functions. to test this, a large set of nss mutants was generated by alanine substitutions in nss from resistance-inducing wild-type strains (nss(ri) ), ... | 2014 | 24103150 |
| broad-spectrum transgenic resistance against distinct tospovirus species at the genus level. | thrips-borne tospoviruses cause severe damage to crops worldwide. in this investigation, tobacco lines transgenic for individual wlm constructs containing the conserved motifs of the l rna-encoded rna-dependent rna polymerase (l) gene of watermelon silver mottle virus (wsmov) were generated by agrobacterium-mediated transformation. the wlm constructs included: (i) translatable wlm in a sense orientation; (ii) untranslatable wlmt with two stop codons; (iii) untranslatable wlmts with stop codons a ... | 2014 | 24811071 |
| development of a molecular assay for the general detection of tospoviruses and the distinction between tospoviral species. | a luminex xtag-based assay for plant-infecting tospoviruses was developed. the test enables the detection of tospoviruses in general and the differentiation of the four important member species of this genus: tomato spotted wilt virus, impatiens necrotic spot virus, the proposed 'capsicum chlorosis virus' and watermelon silver mottle virus. the generic tospovirus primers used in this method are also applicable for detection of tospoviruses by basic rt-pcr. we also describe an economic alternativ ... | 2017 | 28190200 |
| an efficient and high fidelity method for amplification, cloning and sequencing of complete tospovirus genomic rna segments. | tospoviruses (genus tospovirus, family bunyaviridae) are responsible for major losses in an extensive range of crops worldwide. new species of these single-stranded, ambisense rna viruses regularly emerge and have been shown to maintain heterogeneous populations with individual isolates having quite variable biological and virulence characteristics. most tospovirus phylogenetic studies have focused on analysis of a single gene, most often the nucleocapsid protein gene. complete genomic rna segme ... | 2017 | 28082165 |
| development of apple latent spherical virus-based vaccines against three tospoviruses. | apple latent spherical virus (alsv) is characterized by its relatively broad host range, latency in most host plants, and ability to induce gene silencing in host plants. herein, we focus on the above characteristic of alsv and describe our development of alsv vector vaccines against three tospoviruses, namely, impatiens necrotic spot virus (insv), iris yellow spot virus (iysv), and tomato spotted wilt virus (tswv). dna fragments of 201 nt of three tospovirus s-rnas (silencing suppressor (nss) a ... | 2013 | 23850843 |
| development of a real-time fluorescent quantitative pcr assay for detection of impatiens necrotic spot virus. | impatiens necrotic spot virus (insv) is an important plant virus that can cause severe disease in various ornamental and agricultural crops. several species of thrips transmit insv, of which the western flower thrip (frankliniella occidentalis) is the most important. in this study, primers and taqman probes based on insv non-structural protein gene sequences were designed, and a technique was developed for detecting insv using fluorescent quantitative rt-pcr. the reproducibility, specificity and ... | 2013 | 23466630 |
| impeded thrips transmission of defective tomato spotted wilt virus isolates. | two defective rna-containing isolates (pe-1 and 16-2) and an envelope-deficient (env ) isolate of tomato spotted wilt virus (tswv) were tested for their transmissibility by frankliniella occidentalis. the pe-1 isolate contained a truncated l rna segment that barely interfered with symptom expression and replication of the wild-type (wt) l rna segment. this isolate was transmitted with an efficiency of 51%, a value comparable to that found for wt tswv (54%). isolate 16-2, which contained a genuin ... | 2000 | 18944549 |
| virus diseases in lettuce in the mediterranean basin. | lettuce is frequently attacked by several viruses causing disease epidemics and considerable yield losses along the mediterranean basin. aphids are key pests and the major vectors of plant viruses in lettuce fields. lettuce mosaic virus (lmv) is probably the most important because it is seed-transmitted in addition to be transmissible by many aphid species that alight on the crop. tomato spotted wilt virus (tswv) is another virus that causes severe damage since the introduction of its major vect ... | 2012 | 22682170 |
| oxylipin biosynthesis genes positively regulate programmed cell death during compatible infections with the synergistic pair potato virus x-potato virus y and tomato spotted wilt virus. | one of the most severe symptoms caused by compatible plant-virus interactions is systemic necrosis, which shares common attributes with the hypersensitive response to incompatible pathogens. although several studies have identified viral symptom determinants responsible for systemic necrosis, mechanistic models of how they contribute to necrosis in infected plants remain scarce. here, we examined the involvement of different branches of the oxylipin biosynthesis pathway in the systemic necrosis ... | 2013 | 23487466 |
| population genetic analysis of tomato spotted wilt virus on peanut in north carolina and virginia. | exploring the genetic diversity and evolutionary history of plant viruses is critical to understanding their ecology and epidemiology. in this study, maximum-likelihood and population genetics-based methods were used to investigate the population structure, genetic diversity, and sources of genetic variation in field isolates of tomato spotted wilt virus (tswv) from peanut in north carolina and virginia. selected regions of the nucleocapsid, movement, and rna-dependent rna polymerase genes were ... | 2011 | 20839960 |
| matrix attachment regions increase the efficiency and stability of rna-mediated resistance to tomato spotted wilt virus in transgenic tobacco. | matrix attachment regions (mars) are dna elements that can increase and stabilize transgene expression. we investigated the effect of the rb7 mar on transgenic virus resistance. constructs for resistance to tomato spotted wilt virus (tswv) with and without flanking rb7 mars were used to transform tobacco and produce homozygous lines. the population with the mar construct had a significantly higher percentage of tswv resistant plants in the r1 generation than the nonmar population. each resistant ... | 2005 | 16022390 |
| differential identification of three species of curtovirus using loop-mediated isothermal amplification. | rapid and sensitive detection methods for three species of curtovirus were developed using a loop-mediated isothermal amplification (lamp) technique. a universal primer set for detecting the three main species of curtovirus at the same time, and three kinds of species-specific primer sets were designed and used for lamp reactions. results from the lamp reactions were visualized both by color changes after adding sybr green i staining dye and by dna laddering on agarose gel electrophoresis. the o ... | 2014 | 24957721 |
| requirements for er-arrest and sequential exit to the golgi of tomato spotted wilt virus glycoproteins. | the envelope glycoproteins gn and gc are major determinants in the assembly of tomato spotted wilt virus (tswv) particles at the golgi complex. in this article, the er-arrest of singly expressed gc and the transport of both glycoproteins to the golgi upon coexpression have been analyzed.while preliminary results suggest that the arrest of gc at the er (endoplasmic reticulum) did not appear to result from improper folding, transient expression of chimeric gc, in which the transmembrane domain (tm ... | 2009 | 19302268 |
| major tomato viruses in the mediterranean basin. | tomato (solanum lycopersicum l.) originated in south america and was brought to europe by the spaniards in the sixteenth century following their colonization of mexico. from europe, tomato was introduced to north america in the eighteenth century. tomato plants show a wide climatic tolerance and are grown in both tropical and temperate regions around the world. the climatic conditions in the mediterranean basin favor tomato cultivation, where it is traditionally produced as an open-field plant. ... | 2012 | 22682165 |
| cell death triggering and effector recognition by sw-5 sd-cnl proteins from resistant and susceptible tomato isolines to tomato spotted wilt virus. | only a limited number of dominant resistance genes acting against plant viruses have been cloned, and further functional studies of these have been almost entirely limited to the resistance genes rx against potato virus x (pvx) and n against tobacco mosaic virus (tmv). recently, the cell-to-cell movement protein (nsm ) of tomato spotted wilt virus (tswv) has been identified as the avirulence determinant (avr) of sw-5b-mediated resistance, a dominant resistance gene which belongs to the class of ... | 2016 | 27271212 |
| binding of tomato spotted wilt virus to a 94-kda thrips protein. | abstract using protein blot assays, a 94-kda thrips protein was identified that exhibited specific binding to tomato spotted wilt virus (tswv) particles. renaturation of the 94-kda protein, which is conserved among the two major vector species of tswv, frankliniella occidentalis and thrips tabaci, was crucial for its virus-binding properties, whereas under the same conditions no specific binding was observed with aphid (myzus persicae) proteins. the 94-kda protein species was present in all deve ... | 1998 | 18945001 |
| odor, not performance, dictates bemisia tabaci's selection between healthy and virus infected plants. | although, insect herbivores are generally thought to select hosts that favor the fitness of their progeny, this "mother-knows-best" hypothesis may be challenged by the presence of a plant virus. our previous study showed that the whitefly, bemisia tabaci, the obligate vector for transmitting tomato yellow leaf curl virus (tylcv), preferred to settle and oviposit on tylcv-infected rather than healthy host plant, datura stramonium. the performances of b. tabaci larvae and adults were indeed improv ... | 2017 | 28360861 |
| the movement proteins (nsm) of distinct tospoviruses peripherally associate with cellular membranes and interact with homologous and heterologous nsm and nucleocapsid proteins. | tospovirus is the only genus containing virus species which infect plants in the bunyaviridae family. the aims of this study were to understand the in vivo membrane association of the movement protein (nsm) of the tospovirus species bean necrotic mosaic virus, chrysanthemum stem necrosis virus, tomato chlorotic spot virus and tomato spotted wilt virus and the homologous and heterologous interactions among nsm and nucleocapsid protein (n). the results obtained by bimolecular fluorescence compleme ... | 2015 | 25705793 |
| movement and nucleocapsid proteins coded by two tospovirus species interact through multiple binding regions in mixed infections. | negative-stranded tospoviruses (family: bunyaviridae) are among the most agronomically important viruses. some of the tospoviruses are known to exist as mixed infections in the same host plant. iris yellow spot virus (iysv) and tomato spotted wilt virus (tswv) were used to study virus-virus interaction in dually infected host plants. viral genes of both viruses were separately cloned into binary psite-bifc vectors. bifc results showed that the n and nsm proteins of iysv interact with their count ... | 2015 | 25666522 |
| plant 4/1 protein: potential player in intracellular, cell-to-cell and long-distance signaling. | originally isolated as a result of its ability to interact with the movement protein of tomato spotted wilt virus in a yeast two-hybrid system, the 4/1 protein is proving to be an excellent tool for studying intracellular protein trafficking and intercellular communication. expression of 4/1 in vivo is tightly regulated, first appearing in the veins of the cotyledon and later in the vasculature of the leaf and stem in association with the xylem parenchyma and phloem parenchyma. structural studie ... | 2014 | 24611067 |
| distribution of tomato spotted wilt virus in dahlia plants. | tomato spotted wilt virus (tswv) causes significant losses in the production of the ornamental plant dahlia variabilis in japan. the purpose of this study was to examine the distribution of tswv in dahlia plants and identify plant parts that can be used in the selection of tswv-free plants. the distribution of tswv was investigated using reverse transcriptional polymerase chain reaction (rt-pcr) and tissue blot immunoassay. the detection rate of tswv in latent infected compound leaves was the hi ... | 2017 | 28129432 |
| differential proteomics analysis of frankliniella occidentalis immune response after infection with tomato spotted wilt virus (tospovirus). | tomato spotted wilt virus (tswv) is mainly vectored by frankliniella occidentalis pergande, and it potentially activates the vector's immune response. however, molecular background of the altered immune response is not clearly understood. therefore, using a proteomic approach, we investigated the immune pathways that are activated in f. occidentalis larvae after 24 h exposure to tswv. two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2d-ief/sds/page) ... | 2017 | 27810283 |
| divergent evolution of multiple virus-resistance genes from a progenitor in capsicum spp. | plants have evolved hundreds of nucleotide-binding and leucine-rich domain proteins (nlrs) as potential intracellular immune receptors, but the evolutionary mechanism leading to the ability to recognize specific pathogen effectors is elusive. here, we cloned pvr4 (a potyvirus resistance gene in capsicum annuum) and tsw (a tomato spotted wilt virus resistance gene in capsicum chinense) via a genome-based approach using independent segregating populations. the genes both encode typical nlrs and ar ... | 2017 | 27612097 |
| identification of major qtls underlying tomato spotted wilt virus resistance in peanut cultivar florida-ep(tm) '113'. | spotted wilt caused by tomato spotted wilt virus (tswv) is one of the major peanut (arachis hypogaea l.) diseases in the southeastern united states. occurrence, severity, and symptoms of spotted wilt disease are highly variable from season to season, making it difficult to efficiently evaluate breeding populations for resistance. molecular markers linked to spotted wilt resistance could overcome this problem and allow selection of resistant lines regardless of environmental conditions. florida-e ... | 2016 | 27600750 |
| host-specific accumulation and temperature effects on the generation of dimeric viral rna species derived from the s-rna of members of the tospovirus genus. | polygonum ringspot virus (polrsv) is a recently characterized tospovirus reported in italy. northern blot analyses of polrsv infections in nicotiana benthamiana and tomato plants showed that a viral rna species with nearly twice the length of the small genomic rna (s-rna) accumulated abundantly in the former host, but was not detected in the latter. additional assays confirmed that biogenesis of this novel rna species was common to all polrsv isolates tested and also to an isolate of tomato spot ... | 2016 | 27600541 |
| the genus tospovirus: emerging bunyaviruses that threaten food security. | the genus tospovirus is unique within the family bunyaviridae in that it is made up of viruses that infect plants. initially documented over 100 years ago, tospoviruses have become increasingly important worldwide since the 1980s due to the spread of the important insect vector frankliniella occidentalis and the discovery of new viruses. as a result, tospoviruses are now recognized globally as emerging agricultural diseases. tospoviruses and their vectors, thrips species in the order thysanopter ... | 2016 | 27578436 |
| manipulation of frankliniella occidentalis (thysanoptera: thripidae) by tomato spotted wilt virus (tospovirus) via the host plant nutrients to enhance its transmission and spread. | earlier studies have shown that tomato spotted wilt virus (tswv) influences the biology, performance, and behavioral patterns of its vector frankliniella occidentalis pergande. in this study, using capsicum annuum l. as the host plant, we aimed to determine the manipulation of f. occidentalis by tswv through switching of the diet (+ or -tswv) during vector's development. behavioral patterns, fitness, as well as vector performance were evaluated. the specific parameters investigated included long ... | 2016 | 27566527 |
| mapping quantitative trait loci of resistance to tomato spotted wilt virus and leaf spots in a recombinant inbred line population of peanut (arachis hypogaea l.) from sunoleic 97r and nc94022. | peanut is vulnerable to a range of diseases, such as tomato spotted wilt virus (tswv) and leaf spots which will cause significant yield loss. the most sustainable, economical and eco-friendly solution for managing peanut diseases is development of improved cultivars with high level of resistance. we developed a recombinant inbred line population from the cross between sunoleic 97r and nc94022, named as the s-population. an improved genetic linkage map was developed for the s-population with 248 ... | 2016 | 27427980 |
| seehabitat: a server on bioinformatics applications for tospoviruses and other species. | plant viruses are important limiting factors in agricultural productivity. tospovirus is one of the severe plant pathogens, causing damage to economically important food and ornamental crops worldwide through thrips as vectors. database application resources exclusively on this virus would help to design better control measures, which aren't available. seehabitat is a unique and exclusive web based server providing work bench to perform computational research on tospoviruses and its species. see ... | 2016 | 27354938 |
| antiviral rna silencing suppression activity of tomato spotted wilt virus nss protein. | in addition to regulating gene expression, rna silencing is an essential antiviral defense system in plants. triggered by double-stranded rna, silencing results in degradation or translational repression of target transcripts. viruses are inducers and targets of rna silencing. to condition susceptibility, most plant viruses encode silencing suppressors that interfere with this process, such as the tomato spotted wilt virus (tswv) nss protein. the mechanism by which nss suppresses rna silencing a ... | 2016 | 27323202 |
| resistance to tospoviruses in vegetable crops: epidemiological and molecular aspects. | during the past three decades, the economic impact of tospoviruses has increased, causing high yield losses in a variety of crops and ornamentals. owing to the difficulty in combating thrips vectors with insecticides, the best way to limit/prevent tospovirus-induced diseases involves a management strategy that includes virus resistance. this review briefly presents current tospovirus taxonomy, diversity, molecular biology, and cytopathology as an introduction to a more extensive description of t ... | 2016 | 27296139 |
| predictive models for tomato spotted wilt virus spread dynamics, considering frankliniella occidentalis specific life processes as influenced by the virus. | several models have been studied on predictive epidemics of arthropod vectored plant viruses in an attempt to bring understanding to the complex but specific relationship between the three cornered pathosystem (virus, vector and host plant), as well as their interactions with the environment. a large body of studies mainly focuses on weather based models as management tool for monitoring pests and diseases, with very few incorporating the contribution of vector's life processes in the disease dy ... | 2016 | 27159134 |
| sequence characterization, molecular phylogeny reconstruction and recombination analysis of the large rna of tomato spotted wilt virus (tospovirus: bunyaviridae) from the united states. | tomato spotted wilt virus (tswv; tospovirus: bunyaviridae) has been an economically important virus in the usa for over 30 years. however the complete sequence of only one tswv isolate pa01 characterized from pepper in pennsylvania is available. | 2016 | 27038777 |
| salivary gland morphology, tissue tropism and the progression of tospovirus infection in frankliniella occidentalis. | tomato spotted wilt virus (tswv) is transmitted by thrips in a propagative manner; however, progression of virus infection in the insect is not fully understood. the goal of this work was to study the morphology and infection of thrips salivary glands. the primary salivary glands (psg) are complex, with three distinct regions that may have unique functions. analysis of tswv progression in thrips revealed the presence of viral proteins in the foregut, midgut, ligaments, tubular salivary glands (t ... | 2016 | 26999025 |
| clustering and cellular distribution characteristics of virus particles of tomato spotted wilt virus and tomato zonate spot virus in different plant hosts. | tomato spotted wilt virus (tswv) and tomato zonate spot virus (tzsv) are the two dominant species of thrip-transmitted tospoviruses, cause significant losses in crop yield in yunnan and its neighboring provinces in china. tswv and tzsv belong to different serogroup of tospoviruses but induce similar symptoms in the same host plant species, which makes diagnostic difficult. we used different electron microscopy preparing methods to investigate clustering and cellular distribution of tswv and tzsv ... | 2016 | 26786326 |
| identification of three new isolates of tomato spotted wilt virus from different hosts in china: molecular diversity, phylogenetic and recombination analyses. | destructive diseases caused by tomato spotted wilt virus (tswv) have been reported associated with many important plants worldwide. recently, tswv was reported to infect different hosts in china. it is of value to clone tswv isolates from different hosts and examine diversity and evolution among different tswv isolates in china as well as worldwide. | 2016 | 26762153 |
| new mexico and the southwestern us are affected by a unique population of tomato spotted wilt virus (tswv) strains. | tomato spotted wilt virus (tswv) is an important pathogen of many ornamental, greenhouse and agronomic crops worldwide. tswv also causes sporadic problems in a number of crops in new mexico (nm). nucleocapsid gene sequences obtained from six different crop species across the state over four different years were used to characterize the nm tswv population. this analysis shows that nm is affected by a unique tswv population that is part of larger independent population present in the southwestern ... | 2016 | 26721573 |
| evaluation of alternatives to carbamate and organophosphate insecticides against thrips and tomato spotted wilt virus in peanut production. | thrips are important pests of peanut. they cause severe feeding injuries on peanut foliage in the early season. they also transmit tomato spotted wilt virus (tswv), which causes spotted wilt disease. at-plant insecticides and cultivars that exhibit field resistance to tswv are often used to manage thrips and spotted wilt disease. historically, peanut growers used the broad-spectrum insecticides aldicarb (irac class 1a; temik) and phorate (irac class 1b; thimet) for managing thrips and thereby re ... | 2016 | 26637534 |
| development and utilization of indel markers to identify peanut (arachis hypogaea) disease resistance. | peanut diseases, such as leaf spot and spotted wilt caused by tomato spotted wilt virus, can significantly reduce yield and quality. application of marker assisted plant breeding requires the development and validation of different types of dna molecular markers. nearly 10,000 ssr-based molecular markers have been identified by various research groups around the world, but less than 14.5% showed polymorphism in peanut and only 6.4% have been mapped. low levels of polymorphism limit the applicati ... | 2015 | 26617627 |
| grafting on a non-transgenic tolerant tomato variety confers resistance to the infection of a sw5-breaking strain of tomato spotted wilt virus via rna silencing. | rna silencing controls endogenous gene expression and drives defensive reactions against invasive nucleic acids like viruses. in plants, it has been demonstrated that rna silencing can be transmitted through grafting between scions and silenced rootstocks to attenuate virus and viroid accumulation in the scions. this has been obtained mostly using transgenic plants, which may be a drawback in current agriculture. in the present study, we examined the dynamics of infection of a resistance-breakin ... | 2015 | 26496695 |
| diversity of thrips species and vectors of tomato spotted wilt virus in tomato production systems in kenya. | thrips have been recognized as primary vectors of tomato spotted wilt virus (tswv) with frankliniella occidentalis (pergande) reported as the most important and efficient vector, while other species such as thrips tabaci lindeman also include populations that can vector the virus. a study was undertaken to establish the diversity of thrips and presence of vectors for tswv in four major tomato production areas in kenya. the cytochrome oxidase 1 (co1) gene was used to generate sequences from thrip ... | 2015 | 26470099 |
| identification and validation of reference genes for normalization of transcripts from virus-infected arabidopsis thaliana. | real-time quantitative polymerase chain reaction (qpcr) of complementary dna is now a standard method for studies of gene expression. however, qpcr can identify genuine variation only when transcript quantities are accurately normalized to an appropriate reference. to identify the most reliable reference genes for transcript quantification by qpcr, we describe a systematic evaluation of candidate reference genes of arabidopsis thaliana ecotype columbia-0 (col-0). twelve genes were selected for t ... | 2011 | 21091160 |
| explaining loss caused by tomato spotted wilt virus on tobacco with boreal winter weather: a bayesian approach. | in north carolina, tomato spotted wilt virus (tswv) has regularly been reported since 1997, with incidence being the highest in 2002. at the end of each season, a questionnaire is sent to the county agents to report disease losses. tswv reported losses in 1993 to 2007 from 58 counties were available. a county-year combination was considered a case and, in total, 494 cases were analyzed. the winter months' temperature and precipitation significantly explained the reported tswv loss (r(2) = 0.82). ... | 2011 | 21091184 |
| effect of spinosad resistance on transmission of tomato spotted wilt virus by the western flower thrips (thysanoptera: thripidae). | tomato spotted wilt virus (tswv) is transmitted by frankliniella occidentalis (pergande) in a persistent-propagative manner. we previously observed significant results in terms of feeding behavior of spinosad-susceptible (ivf03) and -resistant (spin-r) strains of f. occidentalis using electrical penetration graph. tswv transmission by the two strains was compared in the present study. the results showed that the titer of tswv-n rna (a part of s rna of tswv and encoding the nucleocapsid protein) ... | 2016 | 26377766 |
| thrips transmission of tospoviruses. | one hundred years ago, the disease tomato spotted wilt was first described in australia. since that time, knowledge of this disease caused by tomato spotted wilt virus (tswv) and transmitted by thrips (insects in the order thysanoptera) has revealed a complex relationship between the virus, vector, plant host, and environment. numerous tospoviruses and thrips vectors have been described, revealing diversity in plant host range and geographical distributions. advances in characterization of the t ... | 2015 | 26340723 |
| first complete genome sequence of a tomato spotted wilt virus isolate from the united states and its relationship to other tswv isolates of different geographic origin. | we report the first complete nucleotide sequence of a tomato spotted wilt virus (genus tospovirus, family bunyaviridae) isolate from the united states. the tripartite genome of pa01 consisted of l, m and s rnas of 8914, 4765 and 2984 nt, respectively. similarity percentages in nucleotide and amino acid sequence among pa01 and previously characterized tswv isolates are provided here. phylogenetic analysis on the rna-dependent rna polymerase (rdrp) gene placed pa01 in a different clade from an iso ... | 2015 | 26329831 |
| the silencing suppressor (nss) protein of the plant virus tomato spotted wilt virus enhances heterologous protein expression and baculovirus pathogenicity in cells and lepidopteran insects. | in this work, we showed that cell death induced by a recombinant (vacnss) autographa californica multiple nucleopolyhedrovirus (acmnpv) expressing the silencing suppressor (nss) protein of tomato spotted wilt virus (tswv) was enhanced on permissive and semipermissive cell lines. the expression of a heterologous gene (firefly luciferase) during co-infection of insect cells with vacnss and a second recombinant baculovirus (vagppolhfluc) was shown to increase when compared to single vagppolhfluc in ... | 2015 | 26323262 |
| effects of thrips density, mode of inoculation, and plant age on tomato spotted wilt virus transmission in peanut plants. | spotted wilt caused by tomato spotted wilt virus (tswv; family bunyaviridae; genus tospovirus) is a serious disease of peanut (arachis hypogaea l.) in the southeastern united states. peanut genotypes with field resistance to tswv are effective in suppressing spotted wilt. all commercially available genotypes with field resistance to tswv were developed through conventional breeding. as a part of the breeding process, peanut genotypes are regularly screened under field situations. despite numerou ... | 2015 | 26308816 |
| stable reference gene selection for rt-qpcr analysis in nonviruliferous and viruliferous frankliniella occidentalis. | reverse transcriptase-quantitative polymerase chain reaction (rt-qpcr) is a reliable technique for measuring and evaluating gene expression during variable biological processes. to facilitate gene expression studies, normalization of genes of interest relative to stable reference genes is crucial. the western flower thrips frankliniella occidentalis (pergande) (thysanoptera: thripidae), the main vector of tomato spotted wilt virus (tswv), is a destructive invasive species. in this study, the exp ... | 2015 | 26244556 |
| small rna profiles of wild-type and silencing suppressor-deficient tomato spotted wilt virus infected nicotiana benthamiana. | tospoviruses are plant-infecting viruses belonging to the family bunyaviridae. we used a collection of wild-type, phylogenetically distinct tomato spotted wilt virus isolates and related silencing-suppressor defective mutants to study the effects on the small rna (srna) accumulation during infection of nicotiana benthamiana. our data showed that absence of a functional silencing suppressor determined a marked increase of the total amount of viral srnas (vsrnas), and specifically of the 21 nt cla ... | 2015 | 26047586 |
| complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato sw-5 resistance-breaking and pepper tsw resistance-breaking) from spain. | tomato spotted wilt virus (tswv) occurs worldwide and causes production losses in many important horticultural crops such as tomato and pepper. breeding resistant cultivars has been the most successful method so far for tswv disease control, but only two genes have been found to confer resistance against a wide spectrum of tswv isolates: sw-5 in tomato and tsw in pepper. however, tswv resistance-breaking isolates have emerged in different countries a few years after using resistant cultivars. in ... | 2015 | 26026956 |
| untranslatable tospoviral nss fragment coupled with l conserved region enhances transgenic resistance against the homologous virus and a serologically unrelated tospovirus. | tospoviruses cause severe damages to important crops worldwide. in this study, nicotiana benthamiana transgenic lines carrying individual untranslatable constructs comprised of the conserved region of the l gene (denoted as l), the 5' half of nss coding sequence (nss) or the antisense fragment of whole n coding sequence (n) of watermelon silver mottle virus (wsmov), individually or in combination, were generated. a total of 15-17 transgenic n. benthamiana lines carrying individual transgenes wer ... | 2015 | 25721329 |
| structure and function analysis of nucleocapsid protein of tomato spotted wilt virus interacting with rna using homology modeling. | the nucleocapsid (n) protein of tomato spotted wilt virus (tswv) plays key roles in assembling genomic rna into ribonucleoprotein (rnp), which serves as a template for both viral gene transcription and genome replication. however, little is known about the molecular mechanism of how tswv n interacts with genomic rna. in this study, we demonstrated that tswv n protein forms a range of higher ordered oligomers. analysis of the rna binding behavior of n protein revealed that no specific oligomer bi ... | 2015 | 25540203 |
| genetic dissection of novel qtls for resistance to leaf spots and tomato spotted wilt virus in peanut (arachis hypogaea l.). | peanut is an important crop, economically and nutritiously, but high production cost is a serious challenge to peanut farmers as exemplified by chemical spray to control foliar diseases such as leaf spots and thrips, the vectors of tomato spotted wilt virus (tswv). the objective of this research was to map the quantitative trait loci (qtls) for resistance to leaf spots and tswv in one recombinant inbred line (ril) mapping population of "tifrunner × gt-c20" for identification of linked markers fo ... | 2017 | 28197153 |
| erratum to: complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato sw-5 resistance-breaking and pepper tsw resistance-breaking) from spain. | | 2017 | 27981408 |
| thrips developmental stage-specific transcriptome response to tomato spotted wilt virus during the virus infection cycle in frankliniella occidentalis, the primary vector. | tomato spotted wilt virus (tswv) is transmitted by frankliniella occidentalis in a circulative-propagative manner. little is known about thrips vector response to tswv during the infection process from larval acquisition to adult inoculation of plants. whole-body transcriptome response to virus infection was determined for first-instar larval, pre-pupal and adult thrips using rna-seq. tswv responsive genes were identified using preliminary sequence of a draft genome of f. occidentalis as a refer ... | 2017 | 27835811 |
| supervised learning and prediction of spatial epidemics. | parameter estimation for mechanistic models of infectious disease can be computationally intensive. nsoesie et al. (2011) introduced an approach for inference on infectious disease data based on the idea of supervised learning. their method involves simulating epidemics from various infectious disease models, and using classifiers built from the epidemic curve data to predict which model were most likely to have generated observed epidemic curves. they showed that the classification approach cou ... | 2014 | 25457597 |
| evidence of a tomato spotted wilt virus resistance-breaking strain originated through natural reassortment between two evolutionary-distinct isolates. | a tsw resistance-breaking tomato spotted wilt virus field isolate (tswv-p331) found in northern italy originated via reassortment from two evolutionary distinct tswv strains, as revealed by recombination and phylogenetic analysis. compared to the closest isolate present in the database, p331 nss protein carries an unusually high number of amino acid substitutions, but no differences in the nucleocapsid protein. despite these substitutions, p331 nss is a potent silencing suppressor. as shown by p ... | 2015 | 25433286 |
| small rna profiles from virus-infected fresh market vegetables. | functional small rnas, such as short interfering rnas (sirnas) and micrornas (mirnas), exist in freshly consumed fruits and vegetables. these sirnas can be derived either from endogenous sequences or from viruses that infect them. symptomatic tomatoes, watermelons, zucchini, and onions were purchased from grocery stores and investigated by small rna sequencing. by aligning the obtained small rna sequences to sequences of known viruses, four different viruses were identified as infecting these fr ... | 2014 | 25389086 |
| phylogenetic analysis of tomato spotted wilt virus (tswv) nss protein demonstrates the isolated emergence of resistance-breaking strains in pepper. | resurgence of tomato spotted wilt virus (tswv) worldwide as well as in hungary causing heavy economic losses directed the attention to the factors contributing to the outbreak of this serious epidemics. the introgression of tsw resistance gene into various pepper cultivars seemed to solve tswv control, but widely used resistant pepper cultivars bearing the same, unique resistance locus evoked the rapid emergence of resistance-breaking (rb) tswv strains. in hungary, the sporadic appearance of rb ... | 2015 | 25331341 |
| the first complete genome sequences of two distinct european tomato spotted wilt virus isolates. | tomato spotted wilt virus (tswv) represents a major constraint to the production of important vegetable and ornamental crops in several countries around the world, including those in europe. in spite of their economic importance, european tswv isolates have only been partially characterized, and a complete genome sequence has not been determined yet. in this study, we completed the whole genome sequence of two distinct tswv isolates from italy, p105 and p202/3wt. the sequences of the l and m seg ... | 2015 | 25326756 |