| isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (bubalus bubalis) in argentina. | parainfluenza virus type 3 (piv3) was isolated from dairy buffaloes (bubalus bubalis) naturally affected with respiratory and reproductive clinical conditions. | 2012 | 22716217 |
| studies on the pathogenesis of a chinese strain of bovine parainfluenza virus type 3 infection in balb/c mice. | to date, three genotypes a, b, and c of bovine parainfluenza virus type 3 (bpiv3) have been isolated from cattle and only limited studies on the pathogenesis of the genotype a of bpiv3 infection in calves and laboratory animals have been conducted. the pathogenesis of the genotypes b and c of bpiv3 infection in calves and laboratory animals have not been reported. to alleviate the difficulties associated with sourcing suitable calves for infection studies, the establishment of bpiv3 infection mo ... | 2012 | 22391217 |
| phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from brazil. | genomic fragments of the hn and l genes from brazilian bovine parainfluenza 3 virus (bpiv-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (rt-pcr), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bpi3v. the brazilian isolates revealed a high degree of genomic when compared to sf4/32 prototype strain, within the recently proposed genotype a of bpiv- ... | 2011 | 24031776 |
| identification and genome characterization of genotype b and genotype c bovine parainfluenza type 3 viruses isolated in the united states. | bovine parainfluenza 3 viruses (bpi3v) are respiratory pathogens of cattle that cause disease singly but are often associated with bovine respiratory disease complex (brdc) in conjunction with other viral and bacterial agents. bovine vaccines currently contain bpi3v to provide protection against the virus, but there is no current information regarding the bpi3v strains that are circulating in the u.s. | 2015 | 25976921 |
| efficacy and safety of ban huang oral liquid for treating bovine respiratory diseases. | ban huang oral liquid was developed as a veterinary compound preparation by the lanzhou institute of husbandry and pharmaceutical sciences of the chinese academy of agricultural sciences (caas). the purpose of this study was to determine whether the oral liquid preparation of traditional chinese medicine, ban huang, is safe and effective for treating respiratory diseases in cattle. | 2017 | 28573221 |
| virus detection by pcr following vaccination of naive calves with intranasal or injectable multivalent modified-live viral vaccines. | we evaluated duration of pcr-positive results following administration of modified-live viral (mlv) vaccines to beef calves. twenty beef calves were randomly assigned to either group 1 and vaccinated intranasally with a mlv vaccine containing bovine alphaherpesvirus 1 (bohv-1), bovine respiratory syncytial virus (brsv), and bovine parainfluenza virus 3 (bpiv-3), or to group 2 and vaccinated subcutaneously with a mlv vaccine containing bovine viral diarrhea virus 1 and 2 (bvdv-1, -2), bohv-1, brs ... | 2017 | 28545321 |
| reciprocal complementation of bovine parainfluenza virus type 3 lacking either the membrane or fusion gene. | two defective bovine parainfluenza virus type 3 (bpiv3) strains were generated, one lacking the membrane (m) protein gene and expressing egfp (δm-egfp) and the other lacking the fusion (f) protein gene and expressing mstrawberry (δf-msb), by supplying deficient proteins in trans. when madin-darby bovine kidney (mdbk) cells were co-infected with δm-egfp and δf-msb at a multiplicity of infection (moi) of 0.1, complemented viruses were easily obtained. complemented viruses grew as efficiently as wi ... | 2017 | 28842134 |
| cholesterol-rich lipid rafts play a critical role in bovine parainfluenza virus type 3 (bpiv3) infection. | lipid rafts are specialized lipid domains enriched in cholesterol and sphingolipid, which can be utilized in the lifecycle of numerous enveloped viruses. bovine parainfluenza virustype3 (bpiv3) entry to cell is mediated by receptor binding and membrane fusion, but how lipid rafts in host cell membrane and bpiv3 envelope affect virus infection remains unclear. in this study, we investigated the role of lipid rafts in the different stages of bpiv3 infection. the mdbk cells were treated by methyl-β ... | 2017 | 28654867 |
| identification of candidate protein markers of bovine parainfluenza virus type 3 infection using an in vitro model. | bovine parainfluenza virus type 3 (bpi3v) infections are often asymptomatic, causing respiratory tissue damage and immunosuppression, predisposing animals to severe bacterial pneumonia, the leading cause of bovine respiratory disease (brd) mortality. as with many pathogens, routine bpi3v serology does not indicate the presence of damaged respiratory tissue or active infection. in vitro proteomic marker screening using disease relevant cell models could help identify markers of infection and tiss ... | 2017 | 28619153 |
| a single l288i substitution in the fusion protein of bovine parainfluenza virus type 3 enhances virus growth in semi-suitable cell lines. | the bovine parainfluenza virus type 3 bn-ce vaccine strain was obtained by serial passage of the bn-1 strain in chicken embryonic fibroblasts (cef). we previously identified a substitution (l288i) in the fusion (f) protein between the two strains. to examine the effect of the substitution on cef adaptation and attenuation, we generated a recombinant bn-1 strain with the l288i substitution in the f protein (f(l288i)-egfp). f(l288i)-egfp replicated more efficiently than a recombinant bn-1 strain ( ... | 2017 | 28451903 |
| genomic and antigenic characterization of bovine parainfluenza-3 viruses in the united states including modified live virus vaccine (mlv) strains and field strains from cattle. | this study investigated the genetic and antigenic characterization of parainfluenza-3 virus (pi3v) of cattle. using molecular tests including real time pcr and viral genome sequencing, pi3v strains could be separated into pi3v types, including pi3v a, pi3v b, and pi3v c. isolates from cattle with bovine respiratory disease clinical signs and commercial vaccines in the u.s. with mlv pi3v were typed using these molecular tests. all the mlv vaccine strains tested were pi3v a. in most cases pi3v fie ... | 2017 | 28416404 |
| a parainfluenza virus vector expressing the respiratory syncytial virus (rsv) prefusion f protein is more effective than rsv for boosting a primary immunization with rsv. | live-attenuated pediatric vaccines for intranasal administration are being developed for human respiratory syncytial virus (rsv), an important worldwide pediatric respiratory pathogen that lacks a licensed vaccine or suitable antiviral drug. we evaluated a prime-boost strategy in which primary immunization with rsv was boosted by secondary immunization with rsv or with a chimeric bovine/human parainfluenza virus type 3 (rb/hpiv3) vector expressing the rsv fusion f protein. the vector-expressed f ... | 2020 | 33115876 |
| comparative health assessment of urban and non-urban free-ranging mule deer (odocoileus hemionus) in southeastern british columbia, canada. | the provincial wildlife management agency, british columbia ministry of forests, lands, natural resource operations and rural development, performed a translocation to control the urban mule deer (odocoileus hemionus; umd) overpopulation and supplement the declining non-urban mule deer (numd) population in the kootenay region, british columbia, canada. the objectives of this cross-sectional study were to evaluate the health of the urban and numd populations by comparing pathogen exposure, body c ... | 2018 | 29942678 |
| health status of reintroduced wood bison ( bison bison athabascae): assessing the conservation value of an isolated population in northwestern canada. | a central goal for reintroduced populations of threatened wood bison ( bison bison athabascae) is to maintain them free of diseases of concern, particularly bovine tuberculosis (caused by mycobacterium bovis) and brucellosis (caused by brucella abortus). a wood bison population in southwestern yukon, canada was reintroduced into the wild in 1988, but no health assessment has been done since then. to provide an initial assessment of the health status and, hence, the conservation value of this pop ... | 2019 | 29953313 |
| mycoplasma bovis and viral agents associated with the development of bovine respiratory disease in adult dairy cows. | the etiology and pathologic findings of bovine respiratory disease (brd) in adult dairy cows (n = 35) from a commercial dairy herd in southern brazil were investigated. pulmonary samples were examined for histopathologic patterns and specific features within these patterns, while immunohistochemical (ihc) assays were designed to detect the intralesional antigens of viral infectious disease agents and mycoplasma bovis. pneumonia was diagnosed in 91.4% (32/35) of these cases; neither pneumonia nor ... | 2020 | 31232526 |
| diva metabolomics: differentiating vaccination status following viral challenge using metabolomic profiles. | bovine respiratory disease (brd) is a major source of economic loss within the agricultural industry. vaccination against brd-associated viruses does not offer complete immune protection and vaccine failure animals present potential routes for disease spread. serological differentiation of infected from vaccinated animals (diva) is possible using antigen-deleted vaccines, but during virus outbreaks diva responses are masked by wild-type virus preventing accurate serodiagnosis. previous work by t ... | 2018 | 29621258 |
| influenza d virus in respiratory disease in canadian, province of québec, cattle: relative importance and evidence of new reassortment between different clades. | influenza d virus (idv), a segmented single-stranded negative-sense ribonucleic acid (rna) virus, belongs to the new delta influenza virus genus of the orthomyxoviridae family. cattle were proposed as the natural reservoir of idv in which infection was associated with mild-to-moderate respiratory clinical signs (i.e. cough, nasal discharge and dyspnoea). | 2021 | 33764631 |
| the participation of a malignant catarrhal fever virus and mycoplasma bovis in the development of single and mixed infections in beef and dairy cattle with bovine respiratory disease. | the bovine respiratory disease (brd) complex is a multietiological and multifactorial disease associated with a wide range of viral and bacterial pathogens. this study evaluated the contribution of specific infectious disease agents in the development of brd in cattle from brazil and determined if a virus within the malignant catarrhal fever virus (mcfv) group and mycoplasma bovis, acting individually or in conjunction, can be associated with the development of brd. formalin-fixed paraffin-embed ... | 2021 | 34368279 |
| non-epidermidis coagulase-negative staphylococcus isolated from farm animals can inhibit the hemagglutinating activity of newcastle disease virus and bovine parainfluenza virus type 3. | the embp protein of staphylococcus epidermidis inhibits the hemagglutination of the h1n1 influenza virus and protects birds from a viral respiratory infection. several species of coagulase-negative staphylococcus (cons) are present in the respiratory cavity, particularly in nostrils. we hypothesize that non-epidermidis cons found in animals can have the same function as observed in s. epidermidis. thirty non-epidermidis cons isolates were obtained from poultry, sheep, goat, pig, and dairy cow no ... | 2021 | 33892436 |