| the non-canonical tomato yellow leaf curl virus recombinant that displaced its parental viruses in southern morocco exhibits a high selective advantage in experimental conditions. | recombination events are frequently inferred from the increasing number of sequenced viral genomes, but their impact on natural viral populations has rarely been evidenced. tylcv-is76 is a recombinant (begomovirus,geminiviridae) between the israel strain of tomato yellow leaf curl virus (tylcv-il) and the spanish strain of tomato yellow leaf curl sardinia virus (tylcsv-es) that was generated most probably in the late 1990s in southern morocco (souss). its emergence in the 2000s coincided with th ... | 2016 | 27902403 |
| suppressor of rna silencing encoded by tomato yellow leaf curl virus-israel. | the israeli isolate of tomato yellow leaf curl geminivirus (tylcv-is) is a major tomato pathogen, causing extensive crop losses both in the new and old world. surprisingly, however, little is known about the molecular mechanisms of tylcv-is interactions with tomato cells. here, we have identified a tylcv-is protein, v2, which acts as a suppressor of rna silencing and which is unrelated to presently known viral suppressors. specifically, v2, but not other proteins of tylcv-is, inhibited rna silen ... | 2007 | 16979684 |
| permeabilized mammalian cells as an experimental system for nuclear import of geminiviral karyophilic proteins and of synthetic peptides derived from their nuclear localization signal regions. | the plant-infecting geminiviruses deliver their genome and viral proteins into the host cell nucleus. members of the family geminiviridae possess either a bipartite genome composed of two approximately 2.6 kb dnas or a monopartite genome of approximately 3.0 kb dna. the bipartite genome of bean dwarf mosaic virus (bdmv) encodes several karyophilic proteins, among them the capsid protein (cp) and bv1 (nuclear shuttle protein). a cp is also encoded by the monopartite genome of tomato yellow leaf c ... | 2006 | 16894212 |
| a sensitive method for the quantification of virion-sense and complementary-sense dna strands of circular single-stranded dna viruses. | circular single-stranded dna (ssdna) viruses are the smallest viruses known to infect eukaryotes. high recombination and mutation rates have conferred these viruses with an evolutionary potential that has facilitated their emergence. their damaging effects on livestock (circoviruses) and crops (geminiviruses and nanoviruses), and the ubiquity of anelloviruses in human populations and other mammalian species, have resulted in increased interest in better understanding their epidemiology and infec ... | 2014 | 25241765 |
| begomovirus genetic diversity in the native plant reservoir solanum nigrum: evidence for the presence of a new virus species of recombinant nature. | we examined the native plant host solanum nigrum as reservoir of genetic diversity of begomoviruses that cause the tomato yellow leaf curl disease (tylcd) emerging in southern spain. presence of isolates of all the species and strains found associated with tylcd in this area was demonstrated. mixed infections were common, which is a prerequisite for recombination to occur. in fact, presence of a novel recombinant begomovirus was demonstrated. analysis of an infectious clone showed that it result ... | 2006 | 16580040 |
| mutation of three cysteine residues in tomato yellow leaf curl virus-china c2 protein causes dysfunction in pathogenesis and posttranscriptional gene-silencing suppression. | the nuclear localized c2 protein of the monopartite begomovirus tomato yellow leaf curl virus-china (tylcv-c) contributes to viral pathogenicity. here, we have investigated tylcv-c c2 protein domains that play a role in the phenotype. alignment of the c2 protein with 67 homologues from monopartite and bipartite begomoviruses revealed that a putative zinc-finger motif c36-x1-c38-x7-c46-x6-h53-x4-h58c59 and four potential phosphorylation sites (t52, s61, y68, and s74) are highly conserved. when ex ... | 2002 | 11952122 |
| the involvement of heat shock proteins in the establishment of tomato yellow leaf curl virus infection. | tomato yellow leaf curl virus (tylcv), a begomovirus, induces protein aggregation in infected tomatoes and in its whitefly vector bemisia tabaci. the interactions between tylcv and hsp70 and hsp90 in plants and vectors are necessity for virus infection to proceed. in infected host cells, hsp70 and hsp90 are redistributed from a soluble to an aggregated state. these aggregates contain, together with viral dna/proteins and virions, hsps and components of the protein quality control system such as ... | 2017 | 28360921 |
| flupyradifurone effectively manages whitefly bemisia tabaci med (hemiptera: aleyrodidae) and tomato yellow leaf curl virus (tylcv) in tomato. | the cotton whitefly bemisia tabaci (gennadius) is among the most important pests of numerous crops and a vector of >100 plant viruses, causing significant crop losses worldwide. managing the pest as well as inhibiting the transmission of major viruses such as tomato yellow leaf curl virus (tylcv) is of utmost importance for sustainable yields. the efficacy on both whiteflies and virus transmission of the novel systemic butenolide insecticide flupyradifurone, was investigated in this study. | 2017 | 28345196 |
| insecticidal effects on the spatial progression of tomato yellow leaf curl virus and movement of its whitefly vector in tomato. | commercial management of whitefly-transmitted tomato yellow leaf curl virus (tylcv) typically relies on insecticide control of whitefly vectors as a first line of defense. we quantified this effect in crop tunnel studies, with validation in a tomato field setting. tomato yellow leaf curl virus-infected and bemisia tabaci (gennadius)-infested source plants were planted at the beginning of tunneled rows to serve as inoculum source, so that movement of whiteflies and tylcv symptoms could be tracked ... | 2017 | 28334339 |
| genetic diversity of tomato-infecting tomato yellow leaf curl virus (tylcv) isolates in korea. | epidemic outbreaks of tomato yellow leaf curl virus (tylcv) diseases occurred in greenhouse grown tomato (solanum lycopersicum) plants of busan (tylcv-bus), boseong (tylcv-bos), hwaseong (tylcv-hwas), jeju island (tylcv-jeju), and nonsan (tylcv-nons) in korea during 2008-2009. tomato disease by tylcv has never occurred in korea before. we synthesized the full-length genomes of each tylcv isolate from the tomato plants collected at each area and determined their nucleotides (nt) sequences and ded ... | 2010 | 20963475 |
| the spread of tomato yellow leaf curl virus from the middle east to the world. | the ongoing global spread of tomato yellow leaf curl virus (tylcv; genus begomovirus, family geminiviridae) represents a serious looming threat to tomato production in all temperate parts of the world. whereas determining where and when tylcv movements have occurred could help curtail its spread and prevent future movements of related viruses, determining the consequences of past tylcv movements could reveal the ecological and economic risks associated with similar viral invasions. towards this ... | 2010 | 21060815 |
| comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in nicotiana benthamiana and their correlation with the levels of developmental mirnas. | abstract: background: whitefly-transmitted geminiviruses (begomoviruses) are a major limiting factor for the production of numerous dicotyledonous crops throughout the world. begomoviruses differ in the number of components that make up their genomes and association with satellites, and yet they cause strikingly similar phenotypes, such as leaf curling, chlorosis and stunted plant growth. micrornas (mirnas) are small endogenous rnas that regulate plant growth and development. the study described ... | 2011 | 21592402 |
| clathrin-mediated endocytosis is involved in tomato yellow leaf curl virus transport across the midgut barrier of its whitefly vector. | tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted by the whitefly bemisia tabaci. the circulative translocation of the virus in the insect is known in its broad line. however, transit of tylcv from the digestive tract into the haemolymph is poorly understood. we studied the involvement of clathrin in this process by disrupting the clathrin-mediated endocytosis and the endosome network using inhibitor feeding, antibody blocking and dsrna silencing. we monitored the quantities of ... | 2017 | 28056414 |
| a jasmonate-inducible defense trait transferred from wild into cultivated tomato establishes increased whitefly resistance and reduced viral disease incidence. | whiteflies damage tomatoes mostly via the viruses they transmit. cultivated tomatoes lack many of the resistances of their wild relatives. in order to increase protection to its major pest, the whitefly bemisia tabaci and its transmitted tomato yellow leaf curl virus (tylcv), we introgressed a trichome-based resistance trait from the wild tomato solanum pimpinellifolium into cultivated tomato, solanum lycopersicum. the tomato backcross line bc5s2 contains acylsucrose-producing type-iv trichomes, ... | 2016 | 27920785 |
| elevated o₃ and tylcv infection reduce the suitability of tomato as a host for the whitefly bemisia tabaci. | the effects of elevated atmospheric ozone (o₃) levels on herbivorous insects have been well studied, but little is known about the combined effects of elevated o₃ and virus infection on herbivorous insect performance. using open-top chambers in the field, we determined the effects of elevated o₃ and tomato yellow leaf curl virus (tylcv) infection on wild-type (wt) tomato and 35s tomato (jasmonic acid (ja) defense-enhanced genotype) in association with whitefly, bemisia tabaci gennadius biotype b ... | 2016 | 27916792 |
| the contrasting effects of elevated co2 on tylcv infection of tomato genotypes with and without the resistance gene, mi-1.2. | elevated atmospheric co2 typically enhances photosynthesis of c3 plants and alters primary and secondary metabolites in plant tissue. by modifying the defensive signaling pathways in host plants, elevated co2 could potentially affect the interactions between plants, viruses, and insects that vector viruses. r gene-mediated resistance in plants represents an efficient and highly specific defense against pathogens and herbivorous insects. the current study determined the effect of elevated co2 on ... | 2016 | 27881989 |
| ongoing geographical spread of tomato yellow leaf curl virus. | tomato yellow leaf curl virus (tylcv) seriously impacts tomato production throughout tropical and sub-tropical regions of the world. it has a broad geographical distribution and continues to spread to new regions in the indian and pacific oceans including australia, new caledonia and mauritius. we undertook a temporally-scaled, phylogeographic analysis of all publicly available, full genome sequences of tylcv, together with 70 new genome sequences from australia, iran and mauritius. this reveale ... | 2016 | 27619929 |
| the whitefly bemisia tabaci knottin-1 gene is implicated in regulating the quantity of tomato yellow leaf curl virus ingested and transmitted by the insect. | the whitefly bemisia tabaci is a major pest to agricultural crops. it transmits begomoviruses, such as tomato yellow leaf curl virus (tylcv), in a circular, persistent fashion. transcriptome analyses revealed that b. tabaci knottin genes were responsive to various stresses. upon ingestion of tomato begomoviruses, two of the four knottin genes were upregulated, knot-1 (with the highest expression) and knot-3. in this study, we examined the involvement of b. tabaci knottin genes in relation to tyl ... | 2016 | 27455309 |
| the salicylic acid-mediated release of plant volatiles affects the host choice of bemisia tabaci. | the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) causes serious crop losses worldwide by transmitting viruses. we have previously shown that salicylic acid (sa)-related plant defenses directly affect whiteflies. in this study, we applied exogenous sa to tomato plants in order to investigate the interaction between sa-induced plant volatiles and nonviruliferous b. tabaci b and q or b- and q-carrying tomato yellow leaf curl virus (tylcv). the results showed that exogenous sa caused ... | 2016 | 27376280 |
| the autophagy pathway participates in resistance to tomato yellow leaf curl virus infection in whiteflies. | macroautophagy/autophagy plays an important role against pathogen infection in mammals and plants. however, little has been known about the role of autophagy in the interactions of insect vectors with the plant viruses, which they transmit. begomoviruses are a group of single-stranded dna viruses and are exclusively transmitted by the whitefly bemisia tabaci in a circulative manner. in this study, we found that the infection of a begomovirus, tomato yellow leaf curl virus (tylcv) could activate ... | 2016 | 27310765 |
| analysis of the mild strain of tomato yellow leaf curl virus, which overcomes ty-2 gene-mediated resistance in tomato line h24. | in tomato line h24, an isolate of the mild (mld) strain of tomato yellow leaf curl virus (tylcv-mld [jr:kis]) overcomes ty-2 gene-mediated resistance and causes typical symptoms of tomato yellow leaf curl disease (tylcd). no systemic infection with visible symptoms or accumulation of viral dna in the upper leaves was observed in h24 challenged with another isolate, tylcv-il (tylcv-il [jr:osaka]), confirming that h24 is resistant to the il strain. to elucidate the genomic regions that cause the b ... | 2016 | 27231006 |
| corrigendum: tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperatures. | | 2016 | 27166264 |
| complex recombination patterns arising during geminivirus coinfections preserve and demarcate biologically important intra-genome interaction networks. | genetic recombination is an important process during the evolution of many virus species and occurs particularly frequently amongst begomoviruses in the single stranded dna virus family, geminiviridae. as in many other recombining viruses it is apparent that non-random recombination breakpoint distributions observable within begomovirus genomes sampled from nature are the product of variations both in basal recombination rates across genomes and in the over-all viability of different recombinant ... | 2011 | 21949649 |
| chromosomal rearrangements between tomato and solanum chilense hamper mapping and breeding of the tylcv resistance gene ty-1. | tomato yellow leaf curl disease, a devastating disease of solanum lycopersicum (tomato), is caused by a complex of begomoviruses generally referred to as tomato yellow leaf curl virus (tylcv). almost all breeding for tylcv resistance has been based on the introgression of the ty-1 resistance locus derived from solanum chilense la1969. knowledge about the exact location of ty-1 on tomato chromosome 6 will help in understanding the genomic organization of the ty-1 locus. in this study, we analyze ... | 2011 | 21883550 |
| eugenol confers resistance to tomato yellow leaf curl virus (tylcv) by regulating the expression of slper1 in tomato plants. | tomato yellow leaf curl virus (tylcv) is one of the most devastating plant diseases, and poses a significant agricultural concern because of the lack of an efficient control method. eugenol is a plant-derived natural compound that has been widely used as a food additive and in medicine. in the present study, we demonstrated the potential of eugenol to enhance the resistance of tomato plants to tylcv. the anti-tylcv efficiency of eugenol was significantly higher than that of moroxydine hydrochlor ... | 2016 | 26776605 |
| tomato yellow leaf curl virus (tylcv-il): a seed-transmissible geminivirus in tomatoes. | tomato yellow leaf curl virus (tylcv) is one of the most well-known tomato-infecting begomoviruses and transmitted by bemisia tabaci. seed transmission has previously been reported for some rna viruses, but tylcv has not previously been described as a seed-borne virus. in 2013 and 2014, without whitefly-mediated transmission, tylcv was detected in young tomato plants germinated from fallen fruits produced from tylcv-infected tomato plants in the previous cultivation season. in addition, tylcv-is ... | 2016 | 26743765 |
| advanced engineering of lipid metabolism in nicotiana benthamiana using a draft genome and the v2 viral silencing-suppressor protein. | the transient leaf assay in nicotiana benthamiana is widely used in plant sciences, with one application being the rapid assembly of complex multigene pathways that produce new fatty acid profiles. this rapid and facile assay would be further improved if it were possible to simultaneously overexpress transgenes while accurately silencing endogenes. here, we report a draft genome resource for n. benthamiana spanning over 75% of the 3.1 gb haploid genome. this resource revealed a two-member nbfad2 ... | 2012 | 23300750 |
| adaptive evolution by recombination is not associated with increased mutation rates in maize streak virus. | single-stranded (ss) dna viruses in the family geminiviridae are proving to be very useful in real-time evolution studies. the high mutation rate of geminiviruses and other ssdna viruses is somewhat mysterious in that their dna genomes are replicated in host nuclei by high fidelity host polymerases. although strand specific mutation biases observed in virus species from the geminivirus genus mastrevirus indicate that the high mutation rates in viruses in this genus may be due to mutational proce ... | 2012 | 23268599 |
| tomato yellow leaf curl virus confronts host degradation by sheltering in small/midsized protein aggregates. | tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted by the whitefly bemisia tabaci to tomato and other crops. tylcv proteins are endangered by the host defenses. we have analyzed the capacity of the tomato plant and of the whitefly insect vector to degrade the six proteins encoded by the tylcv genome. tomato and whitefly demonstrated the highest proteolytic activity in the fractions containing soluble proteins, less-in large protein aggregates; a significant decrease of tylcv prot ... | 2016 | 26654789 |
| crispr/cas9-mediated viral interference in plants. | the crispr/cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. recently, crispr/cas9 has been used for targeted genome editing in diverse eukaryotic species. | 2015 | 26556628 |
| insecticides promote viral outbreaks by altering herbivore competition. | while the management of biological invasions is often characterized by a series of single-specieg decisions, invasive species exist within larger food webs. these biotic interactions can alter the impact of control/eradication programs and may cause suppression efforts to inadvertently facilitate invasion spread and impact. we document the rapid replacement of the invasive bemisia middle east-asia minor i (meam1) cryptic biotype by the cryptic mediterranean (med) biotype throughout china and dem ... | 2015 | 26552266 |
| temporal effects of a begomovirus infection and host plant resistance on the preference and development of an insect vector, bemisia tabaci, and implications for epidemics. | persistent plant viruses, by altering phenotypic and physiological traits of their hosts, could modulate the host preference and fitness of hemipteran vectors. a majority of such modulations increase vector preference for virus-infected plants and improve vector fitness, ultimately favouring virus spread. nevertheless, it remains unclear how these virus-induced modulations on vectors vary temporally, and whether host resistance to the pathogen influences such effects. this study addressed the tw ... | 2015 | 26529402 |
| monitoring the dynamics of emergence of a non-canonical recombinant of tomato yellow leaf curl virus and displacement of its parental viruses in tomato. | recombinant viruses are increasingly being reported but the dynamics of their emergence is rarely documented. a new recombinant tomato yellow leaf curl virus (tylcv-is76) was detected for the first time in 2010 in southern morocco (souss). an original diagnostic tool was needed to fit its unusual recombination profile. although is76 was detected following the appearance of tylc symptoms on tolerant tomato plants, symptoms could not be associated to is76 or to a synergy with criniviruses. accordi ... | 2015 | 26519598 |
| effectiveness of cyantraniliprole for managing bemisia tabaci (hemiptera: aleyrodidae) and interfering with transmission of tomato yellow leaf curl virus on tomato. | cyantraniliprole is the second xylem-systemic active ingredient in the new anthranilic diamide class. greenhouse (2006), growth chamber (2007), and field studies (2009-2010) were conducted to determine the efficacy of cyantraniliprole for managing bemisia tabaci (gennadius) biotype b and in interfering with transmission of tomato yellow leaf curl virus (tylcv) by this whitefly. cyantraniliprole applied as soil treatments (200 sc) or foliar sprays (100 od) provided excellent adult whitefly contro ... | 2015 | 26470209 |
| manipulation of host quality and defense by a plant virus improves performance of whitefly vectors. | pathogen-mediated interactions between insect vectors and their host plants can affect herbivore fitness and the epidemiology of plant diseases. while the role of plant quality and defense in mediating these tripartite interactions has been recognized, there are many ecologically and economically important cases where the nature of the interaction has yet to be characterized. the bemisia tabaci (gennadius) cryptic species mediterranean (med) is an important vector of tomato yellow leaf curl viru ... | 2015 | 26470098 |
| a novel route controlling begomovirus resistance by the messenger rna surveillance factor pelota. | tomato yellow leaf curl virus (tylcv) is a devastating disease of tomato (solanum lycopersicum) that can be effectively controlled by the deployment of resistant cultivars. the tylcv-resistant line ty172 carries a major recessive locus for tylcv resistance, designated ty-5, on chromosome 4. in this study, the association between 27 polymorphic dna markers, spanning the ty-5 locus, and the resistance characteristics of individual plants inoculated with tylcv in 51 segregating recombinant populati ... | 2015 | 26448569 |
| tomato yellow leaf curl virus differentially influences plant defence responses to a vector and a non-vector herbivore. | plants frequently engage in simultaneous interactions with diverse classes of biotic antagonists. differential induction of plant defence pathways by these antagonists, and interactions between pathways, can have important ecological implications; however, these effects are currently not well understood. we explored how tomato yellow leaf curl virus (tylcv) influenced the performance of its vector (bemisia tabaci) and a non-vector herbivore (tetranychus urticae) occurring separately or together ... | 2016 | 26436779 |
| evaluating weeds as hosts of tomato yellow leaf curl virus. | bemisia tabaci (gennadius) biotype b transmits tomato yellow leaf curl virus (tylcv), which affects tomato production globally. prompt destruction of virus reservoirs is a key component of virus management. identification of weed hosts of tylcv will be useful for reducing such reservoirs. the status of weeds as alternate hosts of tylcv in florida remains unclear. in greenhouse studies, b. tabaci adults from a colony reared on tylcv-infected tomato were established in cages containing one of four ... | 2015 | 26314055 |
| in silico analysis of gene content in tomato genomic regions mapped to the ty-2 resistance gene. | tomato yellow leaf curl virus is one of the main diseases affecting tomato production worldwide. previous studies have shown that ty-2 is an important resistance gene located between molecular markers c2_at2g28250 (82.3 cm) and t0302 (89.0 cm), and exhibits strong resistance to tomato yellow leaf curl virus in asia. in this study, ty-2 candidate genes were subjected to bioinformatic analysis for the sequenced tomato genome. we identified 69 genes between molecular markers c2_at2g28250 and t0302, ... | 2015 | 26214476 |
| replication of tomato yellow leaf curl virus in its whitefly vector, bemisia tabaci. | tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted exclusively by the whitefly bemisia tabaci in a persistent, circulative manner. replication of tylcv in its vector remains controversial, and thus far, the virus has been considered to be nonpropagative. following 8 h of acquisition on tylcv-infected tomato plants or purified virions and then transfer to non-tylcv-host cotton plants, the amounts of virus inside whitefly adults significantly increased (>2-fold) during the first fe ... | 2015 | 26178995 |
| synthesis and biological activity evaluation of novel amino acid derivatives as potential elicitors against tomato yellow leaf curl virus. | disease caused by tomato yellow leaf curl virus (tylcv) brings serious production losses of cultivated tomato worldwide. in our previous study, two novel amino acid derivatives exerted satisfactory antiviral activities against tylcv. in this study, the variation of tylcv, the transcriptional expression level of ty-1 and the enzyme activities of pod and ppo in tomato were monitored after treatment with two amino acid derivatives to illustrate the antiviral mechanism. the results showed the sympto ... | 2015 | 26162434 |
| assessing the genetic variation of ty-1 and ty-3 alleles conferring resistance to tomato yellow leaf curl virus in a broad tomato germplasm. | tomato yellow leaf curl virus (tylcv) hampers tomato production worldwide. our previous studies have focussed on mapping and ultimately cloning of the tylcv resistance genes ty-1 and ty-3. both genes are derived from solanum chilense and were shown to be allelic. they code for an rna-dependent rna polymerase (rdr) belonging to the rdrγ type defined by a dfdgd catalytic domain. in this study, we first fine-mapped the tylcv resistance in s. chilense la1932, la1960 and la1971. results showed that c ... | 2017 | 26028987 |
| transmission of tomato yellow leaf curl virus by bemisia tabaci as affected by whitefly sex and biotype. | bemisia tabaci is a serious pest of vegetables and other crops worldwide. the most damaging and predominant b. tabaci biotypes are b and q, and both are vectors of tomato yellow leaf curl virus (tylcv). previous research has shown that q outperforms b in many respects but comparative research is lacking on the ability of b and q to transmit viruses. in the present study, we tested the hypothesis that b and q differ in their ability to transmit tylcv and that this difference helps explain tylcv o ... | 2015 | 26021483 |
| recessive resistance derived from tomato cv. tyking-limits drastically the spread of tomato yellow leaf curl virus. | the tomato yellow leaf curl disease (tylcd) causes severe damage to tomato (solanum lycopersicum l.) crops throughout tropical and subtropical regions of the world. tylcd is associated with a complex of single-stranded circular dna plant viruses of the genus begomovirus (family geminiviridae) transmitted by the whitefy bemisia tabaci gennadius (hemiptera: aleyrodidae). the tomato inbred line tx 468-rg is a source of monogenic recessive resistance to begomoviruses derived from the hybrid cv. tyki ... | 2015 | 26008699 |
| tomato plant cell death induced by inhibition of hsp90 is alleviated by tomato yellow leaf curl virus infection. | to ensure a successful long-term infection cycle, begomoviruses must restrain their destructive effect on host cells and prevent drastic plant responses, at least in the early stages of infection. the monopartite begomovirus tomato yellow leaf curl virus (tylcv) does not induce a hypersensitive response and cell death on whitefly-mediated infection of virus-susceptible tomato plants until diseased tomatoes become senescent. the way in which begomoviruses evade plant defences and interfere with c ... | 2016 | 25962748 |
| the tomato yellow leaf curl virus (tylcv) v2 protein interacts with the host papain-like cysteine protease cyp1. | the v2 protein of tomato yellow leaf curl geminivirus (tylcv) is an rna-silencing suppressor that counteracts the innate immune response of the host plant. however, this anti-host defense function of v2 may include targeting of other defensive mechanisms of the plant. specifically, we show that v2 recognizes and directly binds the tomato cyp1 protein, a member of the family of papain-like cysteine proteases which are involved in plant defense against diverse pathogens. this binding occurred both ... | 2012 | 22827939 |
| comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and tylcv infection. | tomato yellow leaf curl virus (tylcv) is a member of the family geminiviridae, genus begomovirus. the virus is a widespread plant virus that causes important economic losses in tomatoes. genetic engineering strategies have increasingly been adopted to improve the resistance of tomatoes to tylcv. | 2016 | 27436092 |
| association of an alphasatellite with tomato yellow leaf curl virus and ageratum yellow vein virus in japan is suggestive of a recent introduction. | samples were collected in 2011 from tomato plants exhibiting typical tomato leaf curl disease symptoms in the vicinity of komae, japan. pcr mediated amplification, cloning and sequencing of all begomovirus components from two plants from different fields showed the plants to be infected by tomato yellow leaf curl virus (tylcv) and ageratum yellow vein virus (ayvv). both viruses have previously been shown to be present in japan, although this is the first identification of ayvv on mainland japan; ... | 2014 | 24424499 |
| recent evolution of a novel begomovirus causing tomato leaf curl disease in the al-batinah region of oman. | for last two decades, begomoviruses (family geminiviridae) have been a major constraint for tomato production in oman, particularly in the al-batinah region, the major agricultural area of oman. farms in the al-batinah region were surveyed during january-march and november-december in 2012 and january-february in 2013. leaf samples of tomato plants showing typical leaf curl disease symptoms were collected and analyzed for begomoviruses. out of fifteen begomovirus clones sequenced, seven were sho ... | 2014 | 24052149 |
| autophagy functions as an antiviral mechanism against geminiviruses in plants. | autophagy is an evolutionarily conserved process that recycles damaged or unwanted cellular components, and has been linked to plant immunity. however, how autophagy contributes to plant immunity is unknown. here we reported that the plant autophagic machinery targets the virulence factor βc1 of cotton leaf curl multan virus (clcumuv) for degradation through its interaction with the key autophagy protein atg8. a v32a mutation in βc1 abolished its interaction with nbatg8f, and virus carrying βc1( ... | 2017 | 28244873 |
| tomato yellow leaf curl virus resistance by ty-1 involves increased cytosine methylation of viral genomes and is compromised by cucumber mosaic virus infection. | tomato yellow leaf curl virus (tylcv) and related begomoviruses are a major threat to tomato production worldwide and, to protect against these viruses, resistance genes from different wild tomato species are introgressed. recently, the ty-1 resistance gene was identified, shown to code for an rna-dependent rna polymerase and to be allelic with ty-3. here we show that upon tylcv challenging of resistant lines carrying ty-1 or ty-3, low virus titers were detected concomitant with the production o ... | 2014 | 25136118 |
| major tomato viruses in the mediterranean basin. | tomato (solanum lycopersicum l.) originated in south america and was brought to europe by the spaniards in the sixteenth century following their colonization of mexico. from europe, tomato was introduced to north america in the eighteenth century. tomato plants show a wide climatic tolerance and are grown in both tropical and temperate regions around the world. the climatic conditions in the mediterranean basin favor tomato cultivation, where it is traditionally produced as an open-field plant. ... | 2012 | 22682165 |
| management of whitefly-transmitted viruses in open-field production systems. | whiteflies are a key pest of crops in open-field production throughout the tropics and subtropics. this is due in large part to the long and diverse list of devastating plant viruses transmitted by these vectors. open-field production provides many challenges to manage these viruses and in many cases adequate management has not been possible. diseases caused by whitefly-transmitted viruses have become limiting factors in open-field production of a wide range of crops, i.e., bean golden mosaic di ... | 2014 | 25410102 |
| new insecticides for management of tomato yellow leaf curl, a virus vectored by the silverleaf whitefly, bemisia tabaci. | greenhouse studies using a randomized complete block design were carried out to evaluate the effect of six insecticides on transmission of tomato yellow leaf curl virus (tylcv) by the silverleaf whitefly, bemisia tabaci biotype b gennadius (hemiptera: aleyrodidae) to tomato, lycopersicon esculentum (miller) (solanales: solanaceae), seedlings that were inoculated with whiteflies from a tylcv colony in cages 3, 7, or 14 d after treatment with insecticide. the purpose was to reveal differences in r ... | 2014 | 25368089 |
| a rapid and efficient method for construction of an infectious clone of tomato yellow leaf curl virus. | tomato yellow leaf curl virus (tylcv), a member of the genus begomovirus, is responsible for one of the most devastating viral diseases in tomato-growing countries and is becoming a serious problem in many subtropical and tropical countries. the climate in korea is getting warmer and developing subtropical features in response to global warming. these changes are being accompanied by tylcv, which is now becoming a large problem in the korean tomato industry. the most effective way to reduce dama ... | 2014 | 25289018 |
| effects of plant virus and its insect vector on encarsia formosa, a biocontrol agent of whiteflies. | in this study, we investigated the tritrophic interactions among a persistently transmitted plant virus, tomato yellow leaf curl virus (tylcv), its insect vector, the sweetpotato whitefly bemisia tabaci, and a parasitoid, encarsia formosa gahan, one of the most extensively used biological control agents. as an emerging invasive pest worldwide, the two most damaging whiteflies are b. tabaci b and q cryptic species. on healthy tomato plants, parasitoid-induced mortality was significantly higher in ... | 2014 | 25096549 |
| tomato yellow leaf curl virus benefits population growth of the q biotype of bemisia tabaci (gennadius) (hemiptera: aleyrodidae). | plant viruses can directly influence their insect vectors, and indirectly through their shared host plant, altering their behavior and performance in a mutualistic or rather antagonistic manner. one of the most studied begomovirus, tomato yellow leaf curl virus (tylcv), may also facilitate the expansion of its vector, the whitefly bemisia tabaci (gennadius). considering the likely expansion of the disease and its major vector, we studied the direct and the indirect effects of a mediterranean iso ... | 2014 | 27193818 |
| three-way interactions between the tomato plant, tomato yellow leaf curl virus, and bemisia tabaci (hemiptera: aleyrodidae) facilitate virus spread. | plant defense responses can greatly affect plant viruses and their herbivore vectors. the current article reports on plant defense responses involving jasmonic acid (ja), salicylic acid (sa), and proteinase inhibitor (pi) in the three-way interaction between tomato plants, tomato yellow leaf curl virus, and the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae). the results showed that feeding by viruliferous b. tabaci increases the longevity and fecundity of nonviruliferous b. tabaci ... | 2014 | 25026648 |
| water balance, hormone homeostasis, and sugar signaling are all involved in tomato resistance to tomato yellow leaf curl virus. | vacuolar water movement is largely controlled by membrane channels called tonoplast-intrinsic aquaporins (tip-aqps). some tip-aqp genes, such as tip2;2 and tip1;1, are up-regulated upon exposure to biotic stress. moreover, tip1;1 transcript levels are higher in leaves of a tomato (solanum lycopersicum) line resistant to tomato yellow leaf curl virus (tylcv) than in those of a susceptible line with a similar genetic background. virus-induced silencing of tip1;1 in the tomato resistant line and th ... | 2014 | 24989233 |
| genetic diversity, host range, and distribution of tomato yellow leaf curl virus in iran. | tomato yellow leaf curl virus (tylcv) is considered one of the most important tomato pathogens in tropical and subtropical regions including iran. during the years 2007 to 2009, a total number of 510 symptomatic and asymptomatic vegetable, ornamental and weed samples were collected from fields and greenhouses in ten provinces of iran. symptoms included stunting, yellowing, leaf curl and flower senescence. pcr with specific primers showed tylcv infection in 184 samples (36%) such as cucumber, pep ... | 2014 | 24957717 |
| bemisia tabaci q carrying tomato yellow leaf curl virus strongly suppresses host plant defenses. | the concurrence of tomato yellow leaf curl virus (tylcv) with the spread of its vector bemisia tabaci q rather than b in china suggests a more mutualistic relationship between tylcv and q. here, we investigated the hypothesis that viruliferous b and q have different effects on plant defenses. we found the fecundity of nonviruliferous b, nonviruliferous q, viruliferous q and viruliferous b was 11.080, 12.060, 10.760, and 11.220 respectively on plants previously attacked by the other biotype, howe ... | 2014 | 24912756 |
| upregulation of probing- and feeding-related behavioural frequencies in bemisia tabaci upon acquisition of tomato yellow leaf curl virus. | the behaviour of insect vectors can be altered by the acquisition of plant viruses. bemisia tabaci, which is the vector of tomato yellow leaf curl virus (tylcv), causes damage to susceptible tomato cultivars. here, the frequencies of several behavioural characteristics related to probing and feeding that are exhibited by non-viruliferous (nv) and tylcv-viruliferous (v) adult b. tabaci were compared using a sandwich-type parafilm cage. | 2014 | 24831051 |
| differential identification of three species of curtovirus using loop-mediated isothermal amplification. | rapid and sensitive detection methods for three species of curtovirus were developed using a loop-mediated isothermal amplification (lamp) technique. a universal primer set for detecting the three main species of curtovirus at the same time, and three kinds of species-specific primer sets were designed and used for lamp reactions. results from the lamp reactions were visualized both by color changes after adding sybr green i staining dye and by dna laddering on agarose gel electrophoresis. the o ... | 2014 | 24957721 |
| the tomato yellow leaf curl virus resistance genes ty-1 and ty-3 are allelic and code for dfdgd-class rna-dependent rna polymerases. | tomato yellow leaf curl virus disease incited by tomato yellow leaf curl virus (tylcv) causes huge losses in tomato production worldwide and is caused by different related begomovirus species. breeding for tylcv resistance has been based on the introgression of multiple resistance genes originating from several wild tomato species. in this study we have fine-mapped the widely used solanum chilense-derived ty-1 and ty-3 genes by screening nearly 12,000 plants for recombination events and generati ... | 2013 | 23555305 |
| rapid spread of a recently introduced virus (tomato yellow leaf curl virus) and its vector bemisia tabaci (hemiptera: aleyrodidae) in liaoning province, china. | in liaoning province, china, tomato yellow leaf curl virus (tylcv) was first detected in 2009 and in only four counties. to quantify the spread of tylcv and to identify potential factors influencing its spread in liaoning province, we assayed for tylcv within 1,055 whiteflies (bemisia tabaci (gennadius) complex) from 74 populations and 29 counties in 2011. the b. tabaci species of these individuals was determined based on molecular markers. tylcv was found in 13 counties (donggang, liaoyang, kaz ... | 2014 | 24665690 |
| frequency-dependent assistance as a way out of competitive exclusion between two strains of an emerging virus. | biological invasions are the main causes of emerging viral diseases and they favour the co-occurrence of multiple species or strains in the same environment. depending on the nature of the interaction, co-occurrence can lead to competitive exclusion or coexistence. the successive fortuitous introductions of two strains of tomato yellow leaf curl virus (tylcv-mld and tylcv-il) in réunion island provided an ideal opportunity to study the invasion of, and competition between, these worldwide emergi ... | 2014 | 24598426 |
| molecular variability and evolution of a natural population of tomato yellow leaf curl virus in shanghai, china. | tomato yellow leaf curl virus (tylcv), belonging to the genus begomovirus of the family geminiviridae, is emerging as the most destructive pathogen of tomato plants. since the first report of tylcv in shanghai, china in 2006, tylcv has spread rapidly to 13 provinces or autonomous regions of china. in this study, the molecular variability and evolution of tylcv were monitored in shanghai from its first upsurge in 2006 until 2010. full-length genomic sequences of 26 isolates were obtained by rolli ... | 2014 | 24510706 |
| a review of the mechanisms and components that determine the transmission efficiency of tomato yellow leaf curl virus (geminiviridae; begomovirus) by its whitefly vector. | begomoviruses are a group of icosahedral single stranded dna viruses exclusively transmitted by the sweet potato whitefly bemisia tabaci in a persistent, circulative manner. in this mode of transmission, begomoviruses are acquired by their insect vector as intact virions from the plant phloem, move along the food canal, foregut and esophagus and reach the midgut where they are absorbed into the hemolymph via the filter chamber. the filter chamber is the site where most of the ingested food is fi ... | 2014 | 24508344 |
| expression and functional characterisation of a soluble form of tomato yellow leaf curl virus coat protein. | tomato yellow leaf curl virus (tylcv), a member of the genus begomovirus within the family geminiviridae, is an important pathogen of tomato in many tropical, subtropical and temperate regions. tylcv is exclusively transmitted by the whitefly bemisia tabaci in a circulative manner. the viral coat protein (cp) has been assumed to play important roles in the entry of tylcv into the insect midgut cells. | 2014 | 24488592 |
| degradation mechanisms of the tomato yellow leaf curl virus coat protein following inoculation of tomato plants by the whitefly bemisia tabaci. | tomato yellow leaf curl virus (tylcv) is a begomovirus infecting tomato cultures worldwide. tylcv is transmitted to plants by the whitefly bemisia tabaci. once in the plant, the virus is subjected to attack by the host-plant defences, which may include sequestration in aggregates, proteolysis, ubiquitination, 26s proteasome degradation and autophagy. elucidating how the virus avoids destruction will make it possible to understand infection and possibly devise countermeasures. | 2014 | 24464776 |
| a bead-based suspension array for the multiplexed detection of begomoviruses and their whitefly vectors. | bead-based suspension array systems enable simultaneous fluorescence-based identification of multiple nucleic acid targets in a single reaction. this study describes the development of a novel approach to plant virus and vector diagnostics, a multiplexed 7-plex array that comprises a hierarchical set of assays for the simultaneous detection of begomoviruses and bemisia tabaci, from both plant and whitefly samples. the multiplexed array incorporates genus, species and strain-specific assays, offe ... | 2014 | 24388931 |
| lamium amplexicaule (lamiaceae): a weed reservoir for tomato yellow leaf curl virus (tylcv) in korea. | after the first identification of tomato yellow leaf curl virus (tylcv) in the southern part of korea in 2008, tylcv has rapidly spread to tomato farms in most regions of korea. from 2008 to 2010, a survey of natural weed hosts that could be reservoirs of tylcv was performed in major tomato production areas of korea. about 530 samples were collected and identified as belonging to 25 species from 11 families. pcr and southern hybridization were used to detect tylcv in samples, and replicating for ... | 2014 | 24327090 |
| facultative symbiont hamiltonella confers benefits to bemisia tabaci (hemiptera: aleyrodidae), an invasive agricultural pest worldwide. | bacterial symbionts infect most insect species, including important pests such as whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae), and often exert important effects on host ecology. the facultative symbiont hamiltonella is found at high frequencies in the b. tabaci med (type: mediterranean-med) in china. the prevalence of this symbiont in natural populations suggests beneficial effects of infection or manipulation of host reproduction. to date, however, no empirical studies on the ... | 2013 | 24280594 |
| complete genome sequence of a new begomovirus associated with yellow mosaic disease of hemidesmus indicus in india. | the complete dna a genome of a virus isolate associated with yellow mosaic disease of a medicinal plant, hemidesmus indicus, from india was cloned and sequenced. the length of dna a was 2825 nucleotides, 35 nucleotides longer than the unit genome of monopartite begomoviruses. comparison of the nucleotide sequence of dna a of the virus isolate with those of other begomoviruses showed maximum sequence identity of 69 % to dna a of ageratum yellow vein china virus (ayvcnv; aj558120) and 68 % with to ... | 2014 | 24276235 |
| v2 of tomato yellow leaf curl virus can suppress methylation-mediated transcriptional gene silencing in plants. | tomato yellow leaf curl virus (tylcv) is a dna virus belonging to the genus begomovirus. tylcv replicates using double-stranded dna intermediates that can become the target of plant transcriptional gene silencing (tgs). here, we show that the v2 protein of tylcv can suppress tgs of a transcriptionally silenced green fluorescent protein (gfp) transgene in nicotiana benthamiana line 16-tgs. through bisulfite sequencing and chop-pcr, we demonstrated that the tylcv v2 can reverse gfp transgene silen ... | 2014 | 24187017 |
| tomato yellow leaf curl virus alters the host preferences of its vector bemisia tabaci. | bemisia tabaci, the whitefly vector of tomato yellow leaf curl virus (tylcv), seriously reduces tomato production and quality. here, we report the first evidence that infection by tylcv alters the host preferences of invasive b. tabaci b (middle east-minor asia 1) and q (mediterranean genetic group), in which tylcv-free b. tabaci q preferred to settle on tylcv-infected tomato plants over healthy ones. tylcv-free b. tabaci b, however, preferred healthy tomato plants to tylcv-infected plants. in c ... | 2013 | 24096821 |
| phylogenetic relationships, recombination analysis, and genetic variability among diverse variants of tomato yellow leaf curl virus in iran and the arabian peninsula: further support for a tylcv center of diversity. | the discovery of five strains of tylcv in iran, including the most well-known and widespread, tylcv-il, spurred a detailed study of the full-length genomes of additional tylcv field isolates and an in-depth analysis of phylogenetic relationships, extent of recombination, and genetic variability of tylcv isolates within iran and throughout the arabian peninsula. phylogenetic analysis of complete genome sequences of tylcv isolates from iran and other countries revealed four monophyletic clusters c ... | 2014 | 24068582 |
| recruitment of the host plant heat shock protein 70 by tomato yellow leaf curl virus coat protein is required for virus infection. | a functional capsid protein (cp) is essential for host plant infection and insect transmission of tomato yellow leaf curl virus (tylcv) and other monopartite begomoviruses. we have previously shown that tylcv cp specifically interacts with the heat shock protein 70 (hsp70) of the virus insect vector, bemisia tabaci. here we demonstrate that during the development of tomato plant infection with tylcv, a significant amount of hsp70 shifts from a soluble form into insoluble aggregates. cp and hsp70 ... | 2013 | 23894631 |
| pcr-rflp-based typing for differentiation of tomato yellow leaf curl virus (tylcv) genotypes from infected host plants in korea. | a polymerase chain reaction (pcr) using two sets of primers designed from published tomato yellow leaf curl virus (tylcv) genomes was developed to distinguish from the tylcv-il groups. the specificity of the two sets of primers was proven by testing against control tylcv genomes and the symptomatic leaves of 34 different tomato cultivars naturally infected with tylcv in greenhouses. one set for tylcv-il strain-specific primers (tylcv-uni-f and tylcv-uni-r) amplified full-length genome fragments ... | 2013 | 23884784 |
| virus infection of a weed increases vector attraction to and vector fitness on the weed. | weeds are important in the ecology of field crops, and when crops are harvested, weeds often become the main hosts for plant viruses and their insect vectors. few studies, however, have examined the relationships between plant viruses, vectors, and weeds. here, we investigated how infection of the weed datura stramonium l. by tomato yellow leaf curl virus (tylcv) affects the host preference and performance of the tylcv vector, bemisia tabaci (gennadius) q. the results of a choice experiment indi ... | 2013 | 23872717 |
| differential effects of an exotic plant virus on its two closely related vectors. | concurrent spread of tomato yellow leaf curl virus (tylcv) with invasion of bemisia tabaci q rather than b in china suggests a more mutualistic relationship between tylcv and q than b. to assess this hypothesis, we quantified the impacts of tylcv on the performance and competitiveness of b and q in the laboratory. the results showed that relative to their non-infected counterparts feeding on cotton (a non-host for tylcv), infected b exhibited significant reductions in life-history traits, wherea ... | 2013 | 23864010 |
| eugenol enhances the resistance of tomato against tomato yellow leaf curl virus. | tomato yellow leaf curl virus disease (tylcvd) causes severe to economic losses in tomato crops in china. the control of tylcvd is based primarily on the use of synthetic insecticide to control its vector whitefly (bemisia tabaci). to look for an alternative method for disease control, we investigated the effect of eugenol on controlling tylcvd. the potential of eugenol to trigger systemic acquired resistance (sar) in tomato (jiangsu 14) plants against tylcv was also investigated. | 2014 | 23852671 |
| highly sensitive serological methods for detecting tomato yellow leaf curl virus in tomato plants and whiteflies. | tomato yellow leaf curl virus (tylcv) is a member of the genus begomovirus in the family geminiviridae, which causes severe losses in tomato production in tropic and subtropic regions. | 2013 | 23647724 |
| within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants. | tomato yellow leaf curl virus (tylcv) is a highly damaging begomovirus native to the middle east. tylcv has recently spread worldwide, recombining with other begomoviruses. recent analysis of mixed infections between tylcv and tomato leaf curl comoros begomovirus (tolckmv) has shown that, although natural selection preserves certain co-evolved intra-genomic interactions, numerous and diverse recombinants are produced at 120 days post-inoculation (dpi), and recombinant populations from different ... | 2013 | 23472190 |
| insect symbiont facilitates vector acquisition, retention, and transmission of plant virus. | tomato yellow leaf curl virus (tylcv) was first detected in china in 2006, following the introduction of bemisia tabaci q into china in 2003. since then, the incidence of tylcv in tomato fields in china has greatly increased as has the abundance and distribution of q whiteflies containing the bacterial symbiont hamiltonella with high frequency. this suggested that the symbiont hamiltonella might associate with the transmission efficiency of tylcv by the whitefly vector. here we report the first ... | 2013 | 23455639 |
| involvement of the hexose transporter gene leht1 and of sugars in resistance of tomato to tomato yellow leaf curl virus. | | 2013 | 23430051 |
| multiple forms of vector manipulation by a plant-infecting virus: bemisia tabaci and tomato yellow leaf curl virus. | for many insect-vectored plant viruses, the relationship between feeding behavior and vector competence may prove integral to an understanding of the epidemiology of the resulting plant disease. while plant-infecting viruses are well known to change host plant physiology in a way that makes them more attractive to vectors, viral manipulation of the vectors themselves has only recently been reported. previous research suggested that the rapid spread of tomato yellow leaf curl virus (tylcv) throug ... | 2013 | 23408638 |
| biological invasions of geminiviruses: case study of tylcv and bemisia tabaci in reunion island. | in the last 20 years, molecular ecology approaches have proven to be extremely useful to identify and assess factors associated with viral emerging diseases, particularly in economically and socially important tropical crops such as maize (maize streak disease) and cassava (cassava mosaic disease). molecular ecology approaches were applied in reunion island to analyze the epidemic of tomato yellow leaf curl disease, which has been affecting the island since the end of the 1990s. before the invas ... | 2012 | 23235470 |
| progressive aggregation of tomato yellow leaf curl virus coat protein in systemically infected tomato plants, susceptible and resistant to the virus. | tomato yellow leaf curl virus (tylcv) coat protein (cp) accumulated in tomato leaves during infection. the cp was immuno-detected in the phloem associated cells. at the early stages of infection, punctate signals were detected in the cytoplasm, while in the later stages aggregates of increasing size were localized in cytoplasm and nuclei. sedimentation of protein extracts through sucrose gradients confirmed that progress of infection was accompanied by the formation of cp aggregates of increasin ... | 2013 | 23099086 |
| molecular identification of a new begomovirus associated with mosaic disease of jatropha curcas l. in nigeria. | we report the complete nucleotide sequence of dna-a of a begomovirus naturally infecting jatropha curcas l. in nigeria. symptoms observed on infected plants were severe mosaic, mottling and blistering of leaves. the virus, which we provisionally name "jatropha mosaic nigeria virus" (jmnv), has a monopartite genome of 2,779 to 2,789 nucleotides. pairwise comparisons of dna-a sequences showed that jmnv had maximum nucleotide sequence identity (72%) with a strain of tomato yellow leaf curl virus. s ... | 2013 | 23074041 |
| whitefly population dynamics and evaluation of whitefly-transmitted tomato yellow leaf curl virus (tylcv)-resistant tomato genotypes as whitefly and tylcv reservoirs. | sweetpotato whitefly, bemisia tabaci (gennadius), and whitefly-transmitted tomato yellow leaf curl virus (tylcv) are major threats to tomato production in the southeastern united states. tylcv was introduced to florida from the caribbean islands and has spread to other southern states of the united states. in georgia, in recent years, the incidence of tylcv has been steadily increasing. studies were conducted to monitor population dynamics of whiteflies in the vegetable production belt of georgi ... | 2012 | 22928328 |
| whitefly-mediated transmission of cotton leaf curl multan betasatellite: evidence for betasatellite encapsidation in coat protein of helper begomoviruses. | cotton leaf curl multan betasatellite (clcumb) is responsible for symptom expression of a devastating disease of cotton in the indian subcontinent. clcumb depends on helper virus replication-associated protein for its replication and on viral coat protein (cp) for its encapsidation. however, no direct evidence of encapsidation of clcumb in viral cp has been available. in the present study, non-viruliferous whiteflies were placed on tomato plants that had been agroinoculated with infectious clone ... | 2013 | 22923008 |
| a developmentally regulated lipocalin-like gene is overexpressed in tomato yellow leaf curl virus-resistant tomato plants upon virus inoculation, and its silencing abolishes resistance. | to discover genes involved in tomato resistance to tomato yellow leaf curl virus (tylcv), we previously compared cdna libraries from susceptible (s) and resistant (r) tomato lines. among the genes preferentially expressed in r plants and upregulated by tylcv infection was a gene encoding a lipocalin-like protein. this gene was termed solanum lycopersicum virus resistant/susceptible lipocalin (slvrslip). the slvrslip structural gene sequence of r and s plants was identical. slvrslip was expressed ... | 2012 | 22843056 |
| upregulation of temperature susceptibility in bemisia tabaci upon acquisition of tomato yellow leaf curl virus (tylcv). | acquisition of plant viruses has various effects on physiological mechanisms in vector insects. bemisia tabaci is the only known vector of tomato yellow leaf curl virus (tylcv), which is a serious virus affecting tomato cultivars. in this study, the lifespan of q1 biotype was compared between non-viruliferous (nv) and tylcv-viruliferous (v) whiteflies. total lifespan from egg to adult death of nv whiteflies was 62.54 days but 10.64 days shorter in v whiteflies. we investigated the temperature su ... | 2012 | 22841829 |
| inhibition of binding of tomato yellow leaf curl virus rep to its replication origin by artificial zinc-finger protein. | previously we demonstrated that inhibition of replication-associated protein (rep) binding to its replication origin by artificial zinc-finger proteins (azps) is a powerful method to prevent plant virus infection in vivo. in the present study, we applied the azp technology to tomato yellow leaf curl virus (tylcv), which is a limiting factor in tomato cultivation worldwide. first, we determined 5'-atcggtgt atcggtgt-3' in the 195-bp intergenic region of the tylcv-israel strain, a strain reported f ... | 2013 | 22576255 |
| rapid spread of tomato yellow leaf curl virus in china is aided differentially by two invasive whiteflies. | tomato yellow leaf curl virus (tylcv) was introduced into china in 2006, approximately 10 years after the introduction of an invasive whitefly, bemisia tabaci (genn.) b biotype. even so the distribution and prevalence of tylcv remained limited, and the economic damage was minimal. following the introduction of q biotype into china in 2003, the prevalence and spread of tylcv started to accelerate. this has lead to the hypothesis that the two biotypes might not be equally competent vectors of tylc ... | 2012 | 22514670 |
| association of tomato leaf curl sudan virus with leaf curl disease of tomato in jeddah, saudi arabia. | tomato is an important vegetable crop and its production is adversely affected by leaf curl disease caused by begomovirus. leaf curl disease is a serious concern for tomato crops caused by begomovirus in jeddah, kingdom of saudi arabia. tomato leaf curl disease has been shown to be mainly caused either by tomato leaf curl sudan virus or tomato yellow leaf curl virus as well as tomato leaf curl oman virus. many tomato plants infected with monopartite begomoviruses were also found to harbor a symp ... | 2016 | 27366765 |
| molecular characterization of a naturally occurring intraspecific recombinant begomovirus with close relatives widespread in southern arabia. | tomato leaf curl sudan virus (tolcsdv) is a single-stranded dna begomovirus of tomato that causes downward leaf curl, yellowing, and stunting. leaf curl disease results in significant yield reduction in tomato crops in the nile basin. tolcsdv symptoms resemble those caused by tomato yellow leaf curl virus, a distinct and widespread begomovirus originating in the middle east. in this study, tomato samples exhibiting leaf curl symptoms were collected from gezira, sudan. the associated viral genome ... | 2014 | 24890736 |
| fine mapping of the tomato yellow leaf curl virus resistance gene ty-2 on chromosome 11 of tomato. | resistances to begomoviruses, including bipartite tomato mottle virus and monopartite tomato yellow leaf curl virus (tylcv), have been introgressed to cultivated tomato (solanum lycopersicum) from wild tomato accessions. a major gene, ty-2 from s. habrochaites f. glabratum accession "b6013," that confers resistance to tylcv was previously mapped to a 19-cm region on the long arm of chromosome 11. in the present study, approximately 11,000 plants were screened and nearly 157 recombination events ... | 2014 | 25076841 |
| the role of corchorus in spreading of tomato yellow leaf curl virus on tomato in jeddah, saudi arabia. | corchorus (corchorus capsularis l. and corchorus olitorius l.) is one of the most important fiber crops grown in tropical and subtropical regions throughout the world. field survey was conducted and naturally infected leaf samples were collected from corchorus and tomato plants in jeddah, saudi arabia. the causal virus was transmitted by whiteflies to tomato plants and begomovirus infection was confirmed by polymerase chain reaction. the complete viral genome and associated betasatellites were a ... | 2016 | 26925440 |