| computer analysis between nucleotide and amino acid sequences of bean golden mosaic virus and those of maize streak, wheat dwarf, chloris striate mosaic, and beet curly top viruses. | bean golden mosaic virus (bgmv) dna 1 and 2 have little sequence homology with maize streak virus (msv), wheat dwarf virus (wdv), and chloris striate mosaic virus (csmv) dnas. bgmv dna 1 and beet curly top virus (bctv) dna are closely related, whereas bgmv dna 2 and bctv dna are not related. direct amino acid homologies of predicted proteins between bgmv orfs and msv orfs, wdv orfs or csmv orfs were 40-50%. bgmv 1l1 and bctv l1, and bgmv il3 and bctv l4 were highly conserved. the sequence taatat ... | 1989 | 2615677 |
| moloney murine leukemia virus tolerance in anti-cd4 monoclonal antibody-treated adult mice. | adult mice injected with moloney murine leukemia virus (m-mulv) 1 day after a short term treatment with anti-cd4 mab developed t cell lymphomas, or sarcomas when rechallenged with moloney murine sarcoma virus (m-msv). neoplastic development was correlated with virus spread, as thymic and peripheral t and b lymphocytes promptly expressed m-mulv-induced ag after virus introduction; no virus-specific ctl generation was detected in mltc. this failure, which was selective for m-mulv-induced ag, persi ... | 1991 | 1833453 |
| mutational analysis of the small intergenic region of maize streak virus. | maize streak virus (msv) is a leafhopper-transmitted geminivirus containing one molecule of circular single-stranded dna of about 2.7 kb. we have tested the infectivity of msv mutants. a deletion of 29 bases in the small intergenic region (sir) did not affect the infectivity of msv. mutants containing insertions of oligonucleotides of up to 32 bases at the asni site in sir were also infectious. however, the infection efficiency of the insertion mutants decreased as the size of the oligonucleotid ... | 1991 | 1853570 |
| dna transfer from agrobacterium to zea mays or brassica by agroinfection is dependent on bacterial virulence functions. | dna transfer from agrobacterium tumefaciens, a soil bacterium, to the non-host graminaceous monocotyle-donous plant zea mays, was analysed using the recently developed technique of agroinfection. agroinfection of z. mays with maize streak virus using strains of a. tumefaciens carrying mutations in the ptic58 virulence region showed an almost absolute dependence on the products of the bacterial virc genes. in contrast, agroinfection of the control host brassica rapa with cauliflower mosaic virus ... | 1989 | 2770696 |
| mouse xenotropic viruses can inhibit cell transformation. | mammalian type c retroviruses can be detected by their induction of foci of cell transformation in s+i - cells. we have noted that certain subtypes of the mouse xenotropic type c retrovirus (mulv) inhibit this cell alteration. this inhibition, associated with intact virions, is irreversible and gives the infected cells a phenotype of uninfected cells. in comparison to the transformed cells, the inhibited cultures showed primarily a decrease in murine sarcoma virus (msv) progeny production concom ... | 1987 | 2820140 |
| optimizing gene expression in bpv-transformed cells: effects of cell type on enhancer/promoter interaction. | we have compared several combinations of enhancers and promoters in expressing the chloramphenicol acetyl transferase gene in transient assays, in mouse c127, the most widely used host cell for the bovine papilloma virus (bpv) expression vector. of the various combinations tested, the unit comprised of the sv40 enhancer and adenovirus type 2 major late promoter (mlp) was the most active in bpv transformed c127 cells. we further demonstrate that untransformed and bpv transformed c127 cells respon ... | 1987 | 2821493 |
| human p45ia1 upstream regulatory sequences expressing the chloramphenicol acetyltransferase gene. effect of ha-msv enhancer and comparison of transient with stable transformation assays. | upstream sequences of the human p450ia1 gene were inserted into a promoterless expression vector (psvo-cat) containing the chloramphenicol acetyltransferase (cat) gene, with and without the harvey murine sarcoma virus (ha-msv) core enhancer, and either plasmid was transfected into human breast carcinoma mcf-7 and mda-231 and mouse hepatoma hepa-1 cell lines. in most instances constitutive and inducible cat activities in the transient cat expression assay were similar (within 3-fold) to those in ... | 1987 | 2825673 |
| host range and symptoms are determined by specific domains of the maize streak virus genome. | we have cloned two distinct symptomatic variants of the geminivirus streak virus from maize plants infected with the nigerian strain (msv-n). following "agroinoculation" to maize plants msv-nm produces narrow, mildly chlorotic discontinuous streaks, whereas msv-ns-infected tissue has wide, severely chlorotic streaks. symptom appearance is delayed following msv-nm inoculation. msv-nm has a narrow host range within the gramineae comprising a fraction of that of the wide host range isolate msv-ns. ... | 1991 | 1994579 |
| infectivity and complete nucleotide sequence of the genome of a south african isolate of maize streak virus. | the complete infectious genome of a south african isolate of the geminivirus maize streak (msv-s) has been cloned, characterized, and sequenced. using an a. tumefaciens ti plasmid delivery system, the cloned -2.7 kb single circular msv component was shown to be necessary and sufficient for infection of maize. based on sequence analysis of the infectious clone, msv-s is highly homologous to the previously characterized kenyan and nigerian isolates. while the genomic organization of msv-s has elem ... | 1988 | 2829117 |
| monoclonal antibody against ifn-gamma inhibits moloney murine sarcoma virus-specific cytotoxic t lymphocyte differentiation. | the role of autochthonous ifn- production was evaluated in immune reactions to moloney murine sarcoma virus (m-msv)-induced tumors which are characterized by spontaneous regression mainly caused by virus-specific ctl activity. a functional ifn- depletion, induced by repeated administration of mab anti-ifn- at the site of virus inoculation, prevented tumor regression in m-msv-injected mice. moreover, this antibody inhibited in vitro both proliferation and differentiation of m-msv-specific t lymph ... | 1988 | 2830339 |
| reduced protein kinase c activity in a ras-resistant cell line derived from ki-msv transformed cells. | we have examined phosphatidylinositol turnover and c-kinase distribution in a flat cellular ras-resistant cell line (c11) derived from kirsten murine sarcoma virus (ki-msv) transformed nih/3t3 cells (dt). this cell type has been shown to express high levels of the p21 ki-ras gene product yet is resistant to the transforming effects of this protein. our data indicate that c11 cells have reduced levels of total c-kinase activity when compared to nih/3t3 cells and do not retain the ability to phosp ... | 1987 | 2830575 |
| the in vitro and in vivo anti-retrovirus activity, and intracellular metabolism of 3'-azido-2',3'-dideoxythymidine and 2',3'-dideoxycytidine are highly dependent on the cell species. | cell lines derived from different species show striking differences in their sensitivity to the cytostatic and anti-retrovirus activity, as well as the intracellular metabolism, of 3'-azido-2',3'-dideoxythymidine (azddthd) and 2',3'-dideoxycytidine (ddcyd). azddthd and ddcyd are considerably more cytostatic to human (i.e. raji, molt/4f, ath8) cell lines than murine (i.e. l1210) cells. the intracellular levels of azddthd 5'-triphosphate and ddcyd 5'-triphosphate formed do not seem related to the ... | 1988 | 2830886 |
| increment of dna topoisomerases in chemically and virally transformed cells. | the activities of topoisomerases i and ii were assayed in subcellular extracts obtained from nontumorigenic balb/c 3t3 a31 and normal rat kidney (nrk) cell lines and from the same cells transformed by benzo[a]pyrene (bp-a31), moloney (m-msv-a31) and kirsten (k-a31) sarcoma viruses, and simian virus 40 (sv-nrk). the enzymatic activity of topoisomerase i was monitored by the relaxation of negatively supercoiled pbr322 dna and by the formation of covalent complexes between 32p-labeled dna and topoi ... | 1988 | 2831071 |
| kirsten murine sarcoma virus abolishes interferon gamma-induced class ii but not class i major histocompatibility antigen expression in a murine fibroblast line. | the effect of infecting fibroblasts with kirsten murine sarcoma virus/murine leukemia virus (ki-msv/mlv) on constitutive and ifn-gamma-induced h-2 antigen expression was investigated. the fibroblasts used were two established cell lines (c3h10t1/2 and balb/c3t3) and fresh embryo fibroblasts from c3h mice. class i antigens were expressed constitutively by balb/c3t3; infection with mlv, msv or the two together had little effect on this constitutive expression. class i antigens (h-2k, h-2d) were st ... | 1988 | 2831293 |
| development of an in vitro model of tumor progression using v-raf and v-raf/v-myc transformed rat liver epithelial cells: correlation of tumorigenicity with the downregulation of specific proteins. | a series of clonal cell lines were derived from rat liver epithelial cells after being infected with a defective retrovirus containing either v-raf (3611-msv) or v-raf/v-myc(j2) together with a helper virus. these clones exhibited a different morphology from the regular cuboid shape of the control cells, infected only with the helper virus. all of the infected cell lines contained at least one full length copy of appropriate proviral dna and expressed comparable levels of v-raf mrna, although on ... | 1990 | 2322386 |
| characterization of the sugarcane streak agent as a distinct geminivirus. | the relationship between maize streak virus and the geminivirus causing streak in sugarcane was investigated. the dna of sugarcane streak virus does not cross-hybridize detectably with that of maize streak virus and vice versa. restriction mapping of native replicative form viral dna (genome size 2.7 kb) and of cloned viral dna, combined with limited sequencing and estimated dna sequence divergence, showed that sugarcane streak virus is as unrelated to maize streak virus and digitaria streak vir ... | 1991 | 2016148 |
| functional studies of newly synthesized benzoic acid derivatives: identification of highly potent retinoid-like activity. | three newly synthesized benzoic acid derivatives (terephthalic acid anilides, chalcone carboxylic acid, and azobenzene carboxylic acid), with a certain structural similarity to retinoic acid, were examined for their retinoid-like bioactivity and their capacity to bind to cellular retinoid binding proteins. two in vitro systems were used to evaluate their retinoid-like bioactivity: inhibition of adipose conversion of st 13 murine preadipose cells and growth promotion of murine sarcoma virus (msv) ... | 1988 | 2836439 |
| in vitro differentiation of rhabdomyosarcomas induced by nickel or by moloney murine sarcoma virus. | in vitro cultures and clonal derivatives have been established from rat rhabdomyosarcomas induced by moloney-murine sarcoma virus (msv) or by nickel sulfide; differentiation ability has been studied as expression of desmin, embryonic and adult myosin isoforms, alpha-actin isoforms and cellular fusion. the two rhabdomyosarcoma models showed different levels of myogenic differentiation. multinucleated myotube-like structures were frequently observed in cultures derived from nickel-induced tumours. ... | 1991 | 2039698 |
| the intergenic region of maize streak virus contains a gc-rich element that activates rightward transcription and binds maize nuclear factors. | maize streak virus (msv) is transcribed bidirectionally from an intergenic region and rightward transcription produces an rna that encodes the coat protein. the intergenic region contains promoter elements required for rightward transcription including an upstream activating sequence (uas) which endows the promoter with full activity in a maize transient expression system. the uas contains two gc-rich repeats (gc boxes) and a long inverted repeat or hairpin with a loop harboring a taatattac sequ ... | 1990 | 2103478 |
| bifunctional activity of transforming growth factor type beta on the growth of nrk-49f cells, normal and transformed by kirsten murine sarcoma virus. | transformation of rat nrk-49f cells (49f) by kirsten murine sarcoma virus (ki-msv) renders these cells (ki-49f cells) capable of autonomous anchorage independent (ai) growth. as compared to nontransformed 49f cells, the transformation by ki-msv does not modify the cell response to transforming growth factor-beta (tgf-beta) in monolayer conditions, but alters it in a i growth conditions. the growth of nontransformed or ki-msv-transformed adherent 49f cells is slowed down by porcine tgf-beta, and ... | 1988 | 2840442 |
| increased formation of unintegrated viral dna in skin fibroblasts from patients with familial polyposis coli shortly after infection with kirsten murine sarcoma virus. | skin fibroblasts from patients with familial polyposis coli (fpc) exhibit increased frequency of morphological transformation, anchorage-independent growth, and enhanced production of viral rna and viral particles after infection with murine sarcoma virus(murine leukemia virus), msv(mlv). these fpc fibroblasts form greater amounts of unintegrated viral dna as compared to normal fibroblasts during the first 4 to 24 hr after infection with kirsten msv(kimlv), suggesting that increased susceptibili ... | 1988 | 2846448 |
| polykaryocytosis induced by amphotropic murine c-type virus (ap129) in sac-cells nonproductively transformed by moloney murine sarcoma virus (mo-msv). | nonproducer cells of stu mouse origin (sac) transformed with moloney murine sarcoma virus (mo-msv) and superinfected with the amphotropic murine c-type virus strain ap 129 unexpectedly formed polykaryocytes. the multinuclear giant cells appeared about 30 days after ap 129 infection as confirmed in three independently performed experiments. the majority of the sac-cells became involved in syncytium formation. the polykaryocytes disappeared during continued culture transfers. various cell lines ob ... | 1985 | 2859766 |
| retrovirus-induced changes in major histocompatibility complex antigen expression influence susceptibility to lysis by cytotoxic t lymphocytes. | retrovirus infection of murine fibroblasts was found to alter the expression of major histocompatibility complex (mhc) antigens. fibroblasts infected with moloney murine leukemia virus (m-mulv) exhibited up to a 10-fold increase in cell surface expression of all three class i mhc antigens. increases in mhc expression resulted in the increased susceptibility of m-mulv-infected cells to lysis by allospecific cytotoxic t lymphocytes (ctl). m-mulv appears to exert its effect at the genomic level, be ... | 1985 | 2411792 |
| potentiating effect of ribavirin on the in vitro and in vivo antiretrovirus activities of 2',3'-dideoxyinosine and 2',3'-dideoxy-2,6-diaminopurine riboside. | 2',3'-dideoxyinosine (ddi) and 2',3'-dideoxy-2,6-diaminopurine riboside (dddapr) are potent and selective inhibitors of human immunodeficiency virus (hiv) replication in mt-4 cells. they are also inhibitory to the transformation of c3h/3t3 cells by moloney murine sarcoma virus (msv). in vivo, they are only marginally effective in delaying msv-induced tumor formation, and mortality associated therewith in newborn nmri mice. when combined with ribavirin, ddi and dddapr become much more effective i ... | 1990 | 2123003 |
| characterization of fos-induced osteogenic tumours and tumour-derived murine cell lines. | osteogenic tumours from c-fos (mt-c-fos-ltr)-transgenic mice and from mice infected with the v-fos-bearing fbr murine osteosarcoma virus (fbr msv) showed close morphological and neoplastic similarities. fos mrna expression was elevated in both types of tumours, and expression of several genes characteristic of differentiated bone cells was either lower, enhanced, or not detectable in comparison to that in normal bone. tumour-derived cell lines showed variable levels of exogenous fos expression; ... | 1990 | 2178137 |
| characterization of chemically and virally transformed variants of madin-darby canine kidney (mdck) epithelial cells. | oncogenic derivatives of madin-darby canine kidney (mdck) cells were isolated in the nude mouse, and nononcogenic anchorage-independent transformants were isolated in vitro following chemical mutagenesis in vitro. these transformed cell lines as well as a moloney sarcoma virus (msv) transformed line were characterized with respect to their serum and anchorage requirements, growth rates, final saturation densities, and sensitivities to contact inhibition. none of these in vitro growth characteris ... | 1985 | 2981894 |
| thyroid hormone modulation of transformation induced by kirsten murine sarcoma virus. | we have investigated the effect of triiodothyronine (t3) on the transformation of normal rat kidney (nrk) cells by the kirsten strain of murine sarcoma virus (ki-msv). when nrk cells were grown and infected with ki-msv in medium lacking t3, the yield of transformed foci was about one-half that observed in the cultures supplemented with t3. individual foci appeared somewhat later in cells grown out in medium devoid of t3. the yield of ki-msv released from transformed nrk cells was lower when thes ... | 1985 | 2983886 |
| two oncogenes in avian carcinoma virus mh2: myc and mht. | the 5.2-kilobase (kb) rna genome of avian carcinoma virus mh2 has the genetic structure 5' - delta gag (0.2 kb)-mht (1.2 kb)-myc (1.4 kb)-c(0.4 kb)-poly (a) (0.2 kb)-3'. delta gag is a partial retroviral core protein, mht and myc are cell-derived mh2-specific sequences, and c is the 3'-terminal retroviral vector sequence. the following results were obtained from the complete nucleotide sequences of the mht and myc genes in mh2. (i) delta gag-mht forms a hybrid gene with a contiguous reading fram ... | 1985 | 2986522 |
| bidirectional transcription of maize streak virus dna and identification of the coat protein gene. | three rna transcripts encoded by maize streak virus dna were detected in polyadenylated rna from virus-infected maize leaves. two of the transcripts, a major 0.9 kb and a minor 1.05 kb rna, were mapped on the virion (+) sense dna and the other minor transcript of 1.2 kb was mapped on the complementary (-) sense dna, demonstrating that transcription of msv dna was bidirectional. the two virion sense transcripts were 3' coterminal at nucleotide 1114 but had 5' termini at nucleotides 2682 and 163 r ... | 1985 | 2414736 |
| mutational analyses of the moloney murine sarcoma virus enhancer. | retroviral enhancers seem to play an integral role in determining tissue tropism. in an attempt to define the sequences essential for moloney murine sarcoma virus (mo-msv) enhancer activity, we have undertaken deletion and insertion mutational analyses of the 72-bp repeats. we demonstrate that, in contrast to simian virus 40 (sv40), the proximal 19 bp of the 72-bp unit of mo-msv are not essential for enhancer function in mouse fibroblasts. our analysis further localizes the 5' boundary of the mo ... | 1985 | 2988891 |
| deletion of the gag region from fbr murine osteosarcoma virus does not affect its enhanced transforming activity. | both fbj murine osteosarcoma virus (fbj-msv) and fbr-msv induce transformation in tissue culture and osteogenic sarcomas in mice. in tissue culture, however, fbr-msv induces larger foci with a shorter latency than those induced by fbj-msv. transformation is dependent on expression of the fos oncogene in fbj-msv and of a gag-fos fusion protein in fbr-msv. we have determined that the gag sequences can be deleted from fbr-msv without affecting the high transforming activity of this virus in compari ... | 1985 | 2991577 |
| monoclonal antibody detects a common surface antigen on two independently established murine moloney sarcoma virus non-producer transformants. | monoclonal antibodies were raised against a cell surface glycoprotein (46k antigen) expressed by a moloney sarcoma virus (mo-msv)-transformed stu mouse cell line (sac). although non-productively transformed sac cells expressed the 46k antigen at their surface, transplantation of sac non-producer cells into immunocompetent mice failed to induce anti-46k antibody production. only transplantation of helper virus-superinfected sac cells led to the development of anti-46k antibodies in addition to an ... | 1985 | 2993478 |
| h-2ld antigen encoded by a recombinant retrovirus genome is expressed on the surface of infected cells. | a recombinant murine retrovirus was constructed which contains, within its genome, a truncated version of the gene encoding the murine h-2ld major histocompatibility antigen. the h-2ld gene, which was inserted 3' of the env splice acceptor site in the recombinant retrovirus msv-neo, lacked the 5' promoter and tata sequences and the 3' transcription termination and polyadenylate addition sites of the normal h-2ld gene. transfection of the msv-neo/h-2ld plasmid (pltv-11) into y-2 cells resulted in ... | 1985 | 2993861 |
| detection of the myristylated gag-raf transforming protein with raf-specific antipeptide sera. | the post-translational modifications of the gag-raf fusion proteins of the 3611 murine sarcoma virus (msv) have been examined by inhibiting glycosylation with tunicamycin and by in vivo labeling with [3h]myristic acid. the results show that p75gag-raf is myristylated but not glycosylated and that p90gag-raf is glycosylated but not myristylated (and is now termed gp90gag-raf). gp90gag-raf expression appeared to become lost during passage of the transformed cells, and consequently does not appear ... | 1985 | 2994296 |
| loss of epidermal growth factor receptors and release of transforming growth factors do not correlate with sarcoma virus-transformation in clonally-related nih/3t3-derived cell lines. | transformation of nih/3t3 cells by kirsten murine sarcoma virus (msv) caused a dramatic reduction in the number of cell-surface receptors for epidermal growth factor (egf). however, the number of egf receptors remained at a very low level in a non-tumourigenic revertant cell line isolated from the virus-transformed cells, indicating that an increase in egf receptors is not a requirement for the phenotypic reversion of kirsten msv-transformed 3t3 cells. serum-free conditioned medium from normal a ... | 1985 | 2994627 |
| autoregulation plus upstream positive and negative control regions associated with transcriptional activation of the mouse p1(450) gene. | 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) is known to interact with a cytosolic receptor and, in turn, activate transcription of the mouse p1(450) gene. various lengths of dna upstream of the p1(450) gene were inserted into the psv0-cat expression vector, with and without addition of the harvey murine sarcoma virus (ha-msv) 72-bp repeat enhancer element. the constructs were cotransfected with psv2-neo into mouse hepatoma wild-type cells and two variant cell lines. one variant is believed to res ... | 1985 | 2997746 |
| the effect of interferon treatment of targets on susceptibility to cytotoxic t-lymphocyte killing: augmentation of allogeneic killing and virus-specific killing relative to viral antigen expression. | the effect on ctl lysis of treatment of ctl targets with ifns has been investigated. treatment of targets for alloreactive ctl with either ifn-alpha beta or ifn-gamma markedly augmented cytotoxicity. cold competition experiments implied that ctl recognized the same target structure on both untreated and ifn-treated cells. this augmented lysis is presumably caused by ifn increasing expression of target mhc antigens. in the case of sfv-specific lysis of sfv-infected fibroblasts, ifn-alpha beta or ... | 1985 | 2416676 |
| detection and preliminary characterization of a transmissible agent inducing autoantibody against golgi-antigen. | a transmissible agent inducing autoantibody has been found in association with tumours induced in stu mice with the progressor strain of moloney sarcoma virus (mo-msv). the activity of the agent detectable in serum from tumour-bearing hosts was expressed by development of autoantibody against golgi-associated evolutionary conserved antigen (weiland et al. 1984). this agent was tentatively designated 'agia', anti-golgi inducing agent, on account of its most remarkable biological activity. concomi ... | 1985 | 2998658 |
| analysis of the cellular proto-oncogene mht/raf: relationship to the 5' sequences of v-mht in avian carcinoma virus mh2 and v-raf in murine sarcoma virus 3611. | the avian carcinoma virus mh2 contains a hybrid gene delta gag-mht with a contiguous open reading frame of 2682 base pairs as well as v-myc and avian helper virus-related sequences. delta gag is a partial retroviral core protein gene while v-mht and v-myc are cell-drived sequences. the v-mht sequence can be divided into two regions: the v-raf-related region at its 3' end contains 969 nucleotides which are 94% related as amino acid sequence to the onc-specific v-raf sequence of murine sarcoma vir ... | 1985 | 3002017 |
| class ii histocompatibility antigen expression in human melanocytes transformed by harvey murine sarcoma virus (ha-msv) and kirsten msv retroviruses. | human melanocytes infected with ki-msv or ha-msv, but not amphotropic mulv, undergo a series of transformation-related changes that are characteristic of malignant melanoma. these are (a) expression of ia antigens, in particular dp, dq, and dr class ii histocompatibility gene products, (b) a transformed morphology and ability to grow in soft agar, and (c) a 5-10-fold increase in the cell surface expression of gd3 ganglioside. however, other characteristics of melanoma, such as independence from ... | 1986 | 2430046 |
| characterization of hyaluronate binding proteins isolated from 3t3 and murine sarcoma virus transformed 3t3 cells. | a hyaluronic acid binding fraction was purified from the supernatant media of both 3t3 and murine sarcoma virus (msv) transformed 3t3 cultures by hyaluronate and immunoaffinity chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis resolved the hyaluronate affinity-purified fraction into three major protein bands of estimated molecular weight (mr,e) 70k, 66k, and 56k which contained hyaluronate binding activity and which were termed hyaluronate binding proteins (habp). hyaluro ... | 1987 | 2440472 |
| nucleotide sequence of exon i of the rat c-k-ras gene. | we have isolated a dna fragment containing exon i of rat cellular proto-oncogene of k-ras and sequenced the exon i. the obtained sequence was compared with that of the corresponding region in viral oncogene of kirsten strain of murine sarcoma virus (ki-msv). the results showed that the exon i sequences of cellular k-ras genes in rat and human cells could encode the same amino acid sequence, while the viral k-ras gene could code for two different amino acids corresponding to the 12th and 37th pos ... | 1986 | 3009041 |
| rescue of presumptive viral information from human cells by a helper oncovirus. | we have attempted to rescue presumptive human endogenous retrovirus(es) by using a competent animal oncovirus as a helper. human melanoma cells (line hmb2) were fused, using polyethylene glycol, with mouse nih-3t3 cells which had been infected and transformed by the harvey murine leukaemia and sarcoma virus complex (mlv and msv). the heteropolykaryons obtained were co-cultivated with fresh nih-3t3 cells; filtered (millipore 0.22 micron) medium from these was used to infect further nih-3t3 cells. ... | 1986 | 3016151 |
| 31p nmr spectra of rodent and avian fibroblasts transformed by rous or kirsten sarcoma viruses. | 31p nmr spectra of normal rodent and avian fibroblasts were compared to those of the same cells transformed either by the rous sarcoma virus (rsv) or by the kirsten sarcoma virus (ki-msv). under physiological conditions, the spectra of living or perchloric acid extracted chicken embryo fibroblasts, rat cell line fr3t3 and mouse cell line c127 did not differ from those of their counterparts transformed by rsv or ki-msv. however, in the case of fr3t3 cells, on shifting from 37 degrees c to 20 degr ... | 1986 | 3017336 |
| the interaction of retrovirus and chemical carcinogen in experimental colon carcinogenesis. | the isolation and characterization of oncogenes from human colon cancer and the recognition of their homology with the ras gene of the harvey and kirsten strain of murine sarcoma virus (msv) led us to investigate the effect of exogenous msv on 1,2 dimethylhydrazine (dmh)-induced colon carcinoma in rats. dmh, 20 mg base/kg, was injected weekly for 10 weeks into sprague-dawley rats. the moloney murine sarcoma virus (msv-m) was injected (200 focus-forming units) intraperitoneally into 15 rats 48 ho ... | 1987 | 3027910 |
| functional activity in vivo of effector t cell populations. iii. protection against moloney murine sarcoma virus (m-msv)-induced tumors in t cell deficient mice by the adoptive transfer of a m-msv-specific cytolytic t lymphocyte clone. | the functional activity of moloney murine sarcoma virus (m-msv)-specific t lymphocytes in vivo was assayed by the i.v. injection of virus-specific t lymphocytes into t cell-deficient "b mice". virus-specific t lymphocytes generated in mixed lymphocyte tumor cell cultures were transferred i.v. into syngeneic "b mice" injected simultaneously at a distant site with the virus. these experiments indicated that a low dose (1 x 10(6) cultured cells) of infused lymphocytes can afford protection. to defi ... | 1987 | 3030766 |
| murine retroviruses control class i major histocompatibility antigen gene expression via a trans effect at the transcriptional level. | moloney murine leukemia virus (m-mulv) and moloney murine sarcoma virus (m-msv) exert a regulatory effect on the class i genes of the murine major histocompatibility complex (mhc). we have previously shown that m-mulv infection of mouse fibroblasts results in a substantial increase in cell surface expression of h-2k, h-2d, and h-2l proteins, whereas m-msv, upon coinfection of the same cells, is apparently able to override the mulv-induced increase in h-2 expression. as a result of this modulatio ... | 1987 | 2441241 |
| biologic and molecular characterization of two newly isolated ras-containing murine leukemia viruses. | a murine sarcoma virus (msv) was recovered from an (nfs x ns.c58v-1) f1 mouse which developed splenic sarcoma and erythroleukemia 6 months after inoculation with a mink cell focus-inducing murine leukemia virus (mulv) isolated from an nfs mouse infected with a wild mouse ecotropic mulv. the msv, designated ns.c58 msv-1, induced foci of transformation in mouse and rat fibroblasts, and inoculation of mice of various strains 2 weeks of age or younger resulted in erythroleukemia and sarcomatous lesi ... | 1987 | 3035212 |
| human bronchial epithelial cells neoplastically transformed by v-ki-ras: altered response to inducers of terminal squamous differentiation. | many human bronchial adenocarcinomas have been shown to contain an activated ki-ras oncogene (rodenhuis et al., n. engl. j. med. 317 929-935, 1987). to test the hypothesis that activated ki-ras may be causally related to human bronchial carcinogenesis, v-ki-ras oncogene was transferred into an established human bronchial epithelial cell line, beas-2b, by infection with kirsten murine sarcoma virus (ki-msv) or by transfection with a plasmid containing the transforming region of ki-msv. these cell ... | 1988 | 3067190 |
| interaction between raf and myc oncogenes in transformation in vivo and in vitro. | 3611 msv, a raf-oncogene-transducing murine retrovirus, induces fibrosarcomas and erythroid hyperplasia in newborn mice after a latency of 4-8 wk. in contrast, new recombinant murine retroviruses carrying the myc oncogene (j-3, j-5 construct viruses) do not induce tumors before greater than 9 wk. a combination of both oncogenes in an infectious murine retrovirus (j-2) induces hematopoietic neoplasms in addition to less prominent fibrosarcomas and pancreatic adenocarcinoma 1-3 wk after inoculatio ... | 1986 | 3084503 |
| insulin-producing cells contain a cell-specific repressor activity that functions through multiple e-box sequences. | the cis-acting dna element known as the e box (consensus sequence caxxtg) plays an important role in the transcription of a number of cell-specifically expressed genes. the rat insulin i gene, for example, contains two such sequences (ieb1 and ieb2) that are recognized specifically by a characteristic beta cell nuclear factor insulin enhancer factor 1 (ief1). to define the role of these elements better, we tested for cooperative interactions between the ieb sequences. transfection experiments we ... | 1992 | 1388727 |
| [adducts of 3'-azido-2,3'-dideoxythymidine 5'-phosphate or 5'-phosphonate as inhibitors of cytopathic effect and transformation of cells under the influence of retroviruses in cell culture]. | inhibition of hiv-1- or hiv-2-induced cytopathicity and (moloney) murine sarcoma virus (msv)-induced cell transformation by amino acid and amino alcohol adducts of either 3'-azido-2',3'-dideoxythymidine 5'-monophosphate (aztmp) or 5'-hydrogenphosphonate (azthp) were investigated. both types of nucleotide adducts inhibited replication of hiv-1 and hiv-2 in mt-4 cells at a 1.5- to 3-fold higher ec50 (50% effective concentration) than azt; and, also, selectivity indexes of these adducts were approx ... | 1992 | 1470177 |
| effect of recombinant human interleukin 2 on the growth of a balb/c sarcoma induced by moloney murine sarcoma virus. | the effect of in vivo administration of recombinant interleukin 2 (ril2) on the growth of a primary female balb/c sarcoma induced by moloney murine sarcoma virus (m-msv) was studied. although low-dose administration of (6,000 ju/mouse x 14 days) ril2 had no effect on the growth of the tumors, high-dose (15,000-80,000 ju/mouse x 14 days) intraperitoneal inoculation of ril2 induced tumor regression, dose-dependently. tumors in mice which received 80,000 ju/mouse/day of ril2 regressed completely 2 ... | 1988 | 3141331 |
| in vitro and in vivo growth control of transformed lymphoid cells expressing plasma membrane galactosyltransferase. | the level of beta 1-4 galactosyltransferase activity was examined in a number of spontaneously, chemically, or virally transformed murine tumor cell lines. increased levels of enzyme activity were observed for the murine myeloma cell line k181 and in vivo mopc 104e. the maloney sarcoma virus (msv) transformed t-cell lymphoma, yc-8, also demonstrated elevated levels of enzyme activity when compared to a second independently msv transformed t stem-cell lymphoma, lstra. cell surface immunofluoresce ... | 1989 | 2510114 |
| myosin isoform expression in rat rhabdomyosarcoma induced by moloney murine sarcoma virus. | myosin isoform expression was analyzed in experimental rhabdomyosarcoma (rms) using monoclonal antibodies (mabs) and immunofluorescence techniques. tumors induced by inoculating newborn rats with moloney murine sarcoma virus (mo-msv) were examined 30-90 days after birth. nine tumors and two lymph node metastases were studied by direct, indirect, and double immunofluorescence assays using a panel of five anti-myosin mabs. the mab bf-45 was specifically reactive with embryonic myosin heavy chain ( ... | 1987 | 3305517 |
| defects in lens fiber differentiation are linked to c-mos overexpression in transgenic mice. | we describe three strains of transgenic mice derived by embryo microinjection of dna consisting of a long terminal repeat (ltr) of moloney murine sarcoma virus (mo-msv) linked to the murine c-mos coding sequences. southern analysis of the genomic dna of these strains suggested that in each case the transgene had integrated at a different chromosomal location. the strains were characterized by dominant changes in secondary lens fiber differentiation. shortly after birth, insufficient elongation o ... | 1987 | 3322940 |
| persistent suppression of virus-specific cytotoxic t cell responses after transient depletion of cd4+ t cells in vivo. | co-administration of soluble ag and anti-cd4 mab has been successfully used to induce long term ag-specific tolerance. the mechanisms underlying persistent immunologic unresponsiveness are unclear. we have now studied whether tolerance toward complex viral ag expressed on moloney sarcoma virus (msv)-transformed tumor cells can be induced when given at the time of severe helper cell depletion. although mice that had been injected with anti-cd4 mab at the time of immunization regained the ability ... | 1989 | 2524530 |
| structure and replication of geminivirus genomes. | the geminiviruses are a group of plant viruses containing single-stranded (ss) dna in particles comprising two quasi-icosahedral units. some are transmitted by whiteflies, others by leafhoppers. comparisons were made of the genome organization and expression of cassava latent virus (clv) and maize streak virus (msv) and beet curly top virus (bctv), each with distinct host range and insect vector species characteristics. from these studies, several indications as to the replication mechanism(s) a ... | 1987 | 3503890 |
| reversibility of lymphokine-induced nk-like activity in virus-specific cytotoxic t-lymphocyte clones. | a limiting dilution microculture system, supplemented with a source of interleukin-2 (il-2), was employed to evaluate the frequency of moloney-murine leukaemia/sarcoma virus (m-mulv/m-msv)-specific cytotoxic t-lymphocyte precursors (ctl-p) which also exhibited nk-like activity. spleen cells, obtained from m-mulv/m-msv regressor mice, were restimulated in bulk secondary mixed leucocyte-tumour cell cultures (mltc), and subsequently plated in a culture medium supplemented with two different superna ... | 1985 | 2578429 |
| neoplastic transformation of human epithelial cells in vitro. | efforts to investigate the progression of events that lead human cells of epithelial origin to become neoplastic in response to carcinogenic agents have been aided by the development of tissue culture systems for propagation of epithelial cells. we have recently developed an in vitro multistep model suitable for the study of human epithelial cell carcinogenesis. primary human epidermal keratinocytes acquired indefinite lifespan in culture but did not undergo malignant conversion in response to i ... | 1989 | 2686534 |
| [development and regression of tumors induced by m-msv virus in mice after administration of rutin and quercetin]. | | 1985 | 3831630 |
| evolutionary relationship of three southern african maize streak virus isolates. | the aligned restriction endonuclease maps of three sequenced maize streak virus isolates, three restriction-mapped southern african maize streak virus isolates, and two other sequenced geminiviruses were used as a means of calculating the sequence divergence between these viruses. the degree of divergence was used to construct a phylogenetic tree for the viruses; this tree agrees well with predictions from sequence comparisons, and so the method can be used to study the relationship of geminivir ... | 1989 | 2722470 |
| synthesis of specific transformation-associated proteins (taps) in two rat cell lines is closely related to the expression of the v-mos gene product of moloney murine sarcoma virus (mo-msv). | the 6m2 cell line was established by transformation of normal rat kidney cells with the ts110 mutant of moloney murine sarcoma virus (mo-msv). p85gag-mos was found to be the only known viral-transforming protein in this cell system. previously, we described the detection in 6m2 cells of rat-specific transformation-associated proteins (taps) using a monoclonal antibody (mc). in this study, we used mc to investigate further the expression of taps at different temperatures and the time course of tu ... | 1987 | 2828268 |
| dibutyryl camp inhibits expression of transformation-related properties in kirsten murine sarcoma virus transformed balb/c-3t3 cells despite continued presence of p21v-ki-ras. | we studied the effects of simultaneous treatment with 0.1 mm n6, o2'-dibutyryl camp (dbcamp) and 1 mm theophylline on several transformation-specific properties and on levels of the kirsten murine sarcoma virus (ki-msv) transforming gene product p21v-ki-ras, in a ki-msv-transformed mouse cell line (balb/c-3t3, clone a31; ka31). the rate of logarithmic growth, cell motility, and final saturation density were reduced in dbcamp-treated ka31 cultures. capabilities for anchorage-independent growth we ... | 1988 | 2835972 |
| the ras oncogene product p21 is not a regulatory component of adenylate cyclase. | harvey (ha-msv) and kirsten (ki-msv) murine sarcoma viruses induce tumours in animals and transform various cells in culture because of the expression of the ras oncogene product, p21 (ref. 1). proto-oncogenes homologous with these genes are highly conserved evolutionarily and activated ras oncogenes have been detected in many human cancers. whether c-ras oncogenes are directly responsible for human carcinogenesis is uncertain; however, it is clear that p21 mediates virus-induced transformation, ... | 1985 | 3929144 |
| construction and characterization of a retroviral vector demonstrating efficient expression of cloned cdna sequences. | we describe the construction and properties of a retroviral expression vector, designated pmv-7, designed to transfer unselected cdnas and produce their encoded proteins in recipient cells. the vector is flanked by the long terminal repeats (ltrs) of the moloney murine sarcoma virus (msv) and contains the selectable drug resistance gene neo under the regulation of the herpes simplex virus (hsv) thymidine kinase (tk) promoter. unique eco ri and hind iii sites facilitate the introduction of sequen ... | 1988 | 2836147 |
| delayed hypersensitivity reactions to virus-augmented syngeneic and allogeneic tumor associated antigens. | delayed hypersensitivity (dh) reactions to intact msv-induced tumor cells or their crude membranes (cm) were studied across the h-2 histocompatibility barrier, using the radioisotopic footpad assay. allogeneic as well as syngeneic tumor cells or their cm produced significant dh reactions in msv-immune mice. cm from these cells after infection with influenza virus induced a stronger tumor-specific dh reaction in immune mice than did cm from uninfected cells. these results indicate that h-2 histoc ... | 1988 | 2839400 |
| anti-retrovirus activity of 3'-fluoro- and 3'-azido-substituted pyrimidine 2',3'-dideoxynucleoside analogues. | the 3'-fluoro-and 3'-azido-substituted derivatives of 2',3'-dideoxythymidine (ddthd), 2',3'-dideoxyuridine (ddurd), 2',3'-dideoxy-5-ethyluridine (ddeturd) and 2',3'-dideoxycytidine (ddcyd) have been synthesized and evaluated for their anti-retrovirus activity [against human immunodeficiency virus (hiv) and murine moloney sarcoma virus (msv)]. based on their 50% effective doses the most potent inhibitors of hiv replication in human mt4 lymphocytes were: fddthd (0.001 microm), azddthd (0.004 micro ... | 1988 | 2840080 |
| sequence homology among the coat proteins of gemini viruses. | the coat proteins of the gemini viruses - african cassava latent virus, tomato golden mosaic virus and maize streak virus - are shown to have reasonable to good amino acid sequence homology. it is suggested that the maize streak virus genome is ancestral and the bipartite genomes of the other viruses evolved from it. | 1985 | 4026858 |
| marked in vivo antiretrovirus activity of 9-(2-phosphonylmethoxyethyl)adenine, a selective anti-human immunodeficiency virus agent. | 9-(2-phosphonylmethoxyethyl)adenine (pmea) is a potent and selective inhibitor of the replication of human immunodeficiency virus (hiv) in vitro in human t-lymphocyte mt-4, h9, and ath8 cells. pmea also inhibits moloney murine sarcoma virus (mo-msv)-induced transformation of murine c3h embryo fibroblasts. moreover, pmea causes a dose-dependent suppression of tumor formation and associated mortality in mice inoculated with mo-msv. at a dose of 50 or 20 mg/kg per day pmea effected a 90-100% protec ... | 1989 | 2911579 |
| functions of the mos oncogene family and associated gene products. | the mos oncogene present in moloney murine sarcoma virus is one of the oldest known oncogenes, yet the identification of its biochemical function both in transformation and as a cellular proto-oncogene has been elusive. only recently have low levels of c-mos transcripts been detected in a specific group of mouse tissues. the c-mos gene is implicated in tumorigenicity by its activation by the insertion of the intracisternal a particle genome in a mouse plasmacytoma. the murine c-mos gene is capab ... | 1986 | 2946405 |
| t-cell immunity to murine moloney sarcoma virus-induced tumours: l3t4+ t cells are necessary for resistance to primary sarcoma growth, but lyt-2+ t cells are required for resistance to secondary tumour cell challenge. | experimental protocols have been devised to deliniate the importance of t-cell subsets in immunity to moloney sarcoma virus-induced tumours using the surface antigens l3t4 and lyt-2 as markers of helper and cytotoxic cells, respectively. because the monoclonal antibodies used have been shown to deplete t-cell subsets in vivo, we have been able to study the role of l3t4+ and lyt-2+ t cells in the primary response to msv for the first time. the results clearly show that l3t4+ t cells are the most ... | 1987 | 2956180 |
| cell-mediated reaction against tumors induced by oncornaviruses. i. kinetics and specificity of the immune response in murine sarcoma virus (msv)-induced tumors and transplanted lymphomas. | | 1972 | 4128690 |
| antigens of tumours induced by naturally occurring murine sarcoma virus (msv-fbj). ii. detection of cell-surface antigens by indirect membrane immunofluorescence. | | 1974 | 4133783 |
| baboon virus isolate m-7 with properties similar to feline virus rd-114. | a virus (m-7) isolated from baboon placental tissue demonstrates many similarities to endogenous feline virus rd-114. immunodiffusion analysis shows a group-specific antigen (gs-1) line of identity between m-7 and rd-114. anti-rd-114 dna polymerase igg inhibits m-7 polymerase by 57% compared to 97% for rd-114. m-7 virus has helper activity as demonstrated by rescue of murine sarcoma virus (msv) from sarcoma-positive leukemia-negative human amnion cells. the host range of the rescued m-7 pseudoty ... | 1974 | 4134740 |
| expression of the major internal viral polypeptide in cells transformed by wild-type and temperature-sensitive murine sarcoma virus. | phenotypic expression of the murine intraspecies and interspecies antigenic determinants of the major type c viral structural 30,000-dalton polypeptide, p30, was measured by radioimmunoassay inhibition in cell lines from different species. uninfected normal rat kidney (nrk) cells did not contain detectable levels of murine intraspecies and interspecies p30 antigen, whereas rat cells transformed by and producing murine sarcoma virus (msv)-moloney leukemia virus (m-msv-mulv) contained high levels ... | 1974 | 4139289 |
| revertants of mouse cells transformed by murine sarcoma virus: flat variants without a rescuable sarcoma virus from a clone of balb/3t3 transformed by kirsten msv. | | 1974 | 4140213 |
| neoplastic transformation of mast cells by abelson-mulv: abrogation of il-3 dependence by a nonautocrine mechanism. | normal mast cells can be propagated in culture when medium is supplemented with interleukin-3 (il-3). we demonstrate that abelson-mulv (ab-mulv) infection of mast cells eliminates dependence on il-3 for growth. by contrast, harvey, balb, and moloney msv, which also productively infect mast cells, are unable to relieve il-3 dependence. ab-mulv-induced il-3-independent lines express the v-abl-specific transforming protein and have phenotypic characteristics of mast cells. these cells also possess ... | 1985 | 2988783 |
| presence of circulating antibodies against gag-gene mulv proteins in patients with autoimmune connective tissue disorders. | an immunoblotting procedure using viral proteins from purified murine sarcoma virus or msv-(mlv) has been developed to characterize antiviral antibodies in sera from patients with autoimmune connective tissue disorders. fifty-eight sera with anti-sm, anti-rnp, anti-ss-b (la), and other undefined specificities were found to react with several major viral polypeptide bands. most of them corresponded to gag-gene-encoded products: pr65gag, p40gag, p30, p15, p12 and p10. other bands with molecular we ... | 1985 | 2998055 |
| transformation of a cloned renal epithelial cell line by moloney murine sarcoma virus (m-msv). | | 1970 | 4142389 |
| abelson virus drives the differentiation of harvey virus-infected erythroid cells. | abelson murine leukemia virus (a-mulv) and harvey murine sarcoma virus (ha-msv) are retroviruses carrying unrelated onc genes. however, both of these viruses are capable of stimulating the growth and differentiation of erythroid precursor cells; the target cells for both appear at the same time during fetal development and follow a similar pattern throughout ontogeny. in addition, the colonies induced by each virus are morphologically similar and synthesize the adult form of hemoglobin. however, ... | 1986 | 3002634 |
| [ultrastructural studies of a murine sarcoma virus (msv)]. | | 1967 | 4295344 |
| the differential action of neonatal thymectomy in mice infected with murine sarcoma virus-harvey (msv-h). | | 1968 | 4302084 |
| susceptibility of praomys (mastomys) natalensis to the murine sarcoma virus-harvey (msv-h). | | 1968 | 4302086 |
| replication of murine sarcoma virus-harvey (msv-h) in tissue cultures of virus-induced sarcomas. | | 1968 | 4304098 |
| enhancing effect of the murine sarcoma virus (msv) on the replication of the mouse hepatitis virus (mhv) in vitro. | | 1969 | 4306053 |
| [antigenic properties of a "graffi" pseudotype of the murine rhabdomyosarcoma virus (msv)]. | | 1969 | 4306855 |
| [the role of immunity in the rejection of tumours induced by the msv virus. the effect of antithymocyte serums]. | | 1968 | 4307754 |
| biological activity and separation of a leukaemogenic virus from murine sarcoma virus-harvey (msv-h). | | 1969 | 4310639 |
| murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. | a continuous cell line of highly contact-inhibited cells (nih/3t3) has been developed from nih swiss mouse embryo cultures. its growth properties are similar to those of 3t3 and balb/3t3. although 3t3 is relatively insensitive to focus formation by murine sarcoma viruses, cloned lines of both nih/3t3 and balb/3t3 have been isolated that are highly sensitive to sarcoma virus focus formation and leukemia virus growth. the sensitivity and specificity are comparable to those found with primary embry ... | 1969 | 4311790 |
| homologous recognition of a promoter domain common to the msv ltr and the hsv tk gene. | we have partially purified, from rat liver, a nuclear protein fraction with sequence-specific affinity to a promoter domain shared by the herpesvirus tk gene and the moloney murine sarcoma virus ltr. for both promoters, the protein-binding domain occurs roughly 80 bp upstream of the mrna cap site and harbors the pentanucleotide sequence 5'-ccaat-3'. the msv ltr pentanucleotide occurs on the coding strand in an orientation pointing toward the retroviral transcription unit. the hsv tk pentanucleot ... | 1986 | 3004739 |
| host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. | murine leukemia virus strains fall into three categories with respect to their ability to propagate in cells of national institutes of health (nih) swiss and balb/c mouse embryos. cultures of nih cells are 100- to 1,000-fold more sensitive to "n-tropic" strains than balb/c cell cultures, but are 30- to 100-fold less sensitive to "b-tropic" strains. some virus strains (dually tropic or "nb-tropic") propagate equally well in both cells. m-msv pseudotypes show the host-range characteristics of the ... | 1970 | 4317349 |
| complementary nuclear rna's of murine sarcoma-leukemia virus complex in transformed cells. | the high molecular weight rna (s(20,w) = 69s) of murine sarcomaleukemia virus (msv-mlv) complex dissociated into 37s subunits when heated to 95 degrees c for 2 minutes. specific annealing tests revealed that the viral rna had complementarity with two heterogeneous rna species from the nuclei of cells (78 a1) transformed by and chronically infected with this virus complex. the two nuclear rna species that hybridized with the viral rna had sedimentation constants ranging from 31-36s and 18-22s, re ... | 1969 | 4318348 |
| [transplantable osteosarcoma caused by the "moloney strain of murine sarcoma virus (m-msv)" in osborne-mendel rats]. | | 1970 | 4319060 |
| immunity to murine sarcoma virus (msv)-induced tumors demonstrated by "in vivo" elimination of 51-chromium labeled tumor cells. | | 1970 | 4322224 |
| nucleic acid and proteins isolated from a strain of murine sarcoma virus (msv-o). | | 1971 | 4327113 |
| isolation of a rat-tropic helper virus from m-msv-o stocks. | | 1971 | 4328487 |
| structure and expression of mos sequences in spontaneous and moloney murine sarcoma virus-induced yolk sac carcinomas in rats. | facilitation of yolk-sac carcinoma (ysca) development in fetectomized rats by the moloney murine sarcoma virus/murine leukemia virus (mo-msv/mlv) complex was found to be closely associated with the presence of mo-msv sequences in the genomes of the ysca cells. the virus-induced yscas consisted of cells of mono- or oligoclonal origin which always contained in their genomes at least i randomly integrated mo-msv provirus. in yscas which developed in the absence of virus, no rearrangement or amplifi ... | 1987 | 3030942 |
| clonal isolation of murine sarcoma virus (msv): characterization of virus produced from transformed cells. | | 1971 | 4332977 |
| the murine sarcoma virus (msv). | | 1971 | 4333859 |