| susceptibility to herpesvirus saimiri and antibody development in old and new world monkeys. | | 1973 | 4632986 |
| transplantation and cytogenetic studies of herpesvirus saimiri-induced disease in marmoset monkeys. | | 1973 | 4633909 |
| lymphoma induction and viral isolation from owl monkey (aotus trivirgatus) inoculated with lymphoma cells induced by herpesvirus saimiri. | | 1973 | 4634067 |
| effect of cytosine arabinoside, adenine arabinoside, tilorone, and rifamycin sv on multiplication of herpesvirus saimiri in vitro. | the multiplication of herpesvirus saimiri was inhibited in vitro by cytosine arabinoside, adenine arabinoside, and tilorone, but not by rifamycin sv. | 1972 | 4670435 |
| herpesvirus saimiri malignant lymphoma in spider monkeys. a new susceptible host. | | 1972 | 4680380 |
| herpesvirus saimiri. 3. plaque formation under multi agar, methyl cellulose and starch overlays. | | 1971 | 4995139 |
| morphological studies on herpesvirus saimiri in subhuman and human cell cultures. | | 1971 | 4997845 |
| oncogenicity of herpesvirus saimiri in marmoset monkeys. | | 1971 | 5001259 |
| herpesvirus saimiri induced malignant lymphoma: recovery of the viral agent from the fatally affected animals. | | 1970 | 5006226 |
| acute lymphocytic leukemia in owl monkeys inoculated with herpesvirus saimiri. | our study demonstrates for the first time that herpesvirus saimiri can induce acute lymphocytic leukemia in owl monkeys (aotus trivirgatus) and that malignant lymphoma can be induced in this species of nonhuman primates by the inoculation of the virus by various routes (intravenous, sub-cutaneous, and intradermal). | 1971 | 5544872 |
| propagation of herpesvirus saimiri in human cells. | | 1971 | 5570118 |
| [lymphocyte heterogeneity of the t system: characterization of an allotypic antiserum recognizing a sub-species antigenic determinant in non-human primates]. | to determine the membrane properties in t-lymphocyte population from saguinus primates or lymphoblastoid cell lines of the same origin, an allotypic antiserum was prepared inoculating two saguinus illigeri with 3.5 x 10(10) viable 70n2 cells obtained in-vitro, from a lymphoma induced by inoculation of herpesvirus saimiri in a saguinus oedipus. the 70n2 cells carried the virus genome in an episomal way; it did not produce infectious virus and it formed spontaneous rosettes (99%) with sheep erythr ... | 1982 | 6086023 |
| a single major immediate-early virus gene product is synthesized in cells productively infected with herpesvirus saimiri. | a major virus-specific immediate-early (ie) polypeptide with an apparent mol. wt. of 52 000 (52k) was synthesized immediately after the removal of a cycloheximide block applied to owl monkey kidney cell cultures at the time of infection with high multiplicities of herpesvirus saimiri (hvs). the ie 52k polypeptide was phosphorylated and accumulated rapidly and efficiently in the nucleus of infected cells. monoclonal antibodies to ie 52k and to a major non-structural delayed-early (de) dna-binding ... | 1984 | 6086813 |
| immediate-early transcription of herpesvirus saimiri. | transcription of herpesvirus saimiri was characterized during the initial phases of productive infection by northern blot analyses and hybridizations of radioactive cdna with cloned fragments of virion l-dna. under conditions of immediate-early transcription, e.g., blocking of viral protein synthesis by cycloheximide, a single cytoplasmic polyadenylated viral rna of 2.7 kilobases was found in infected cells. the sequence coding for this rna was between map units 0.89 and 0.93; it was transcribed ... | 1984 | 6086956 |
| transformation of owl monkey t cells in vitro with herpesvirus saimiri. | owl monkey peripheral blood mononuclear cells were treated with phytohemagglutinin and expanded in interleukin 2 (il-2)-containing medium. the cells were then exposed to herpesvirus saimiri (hvs strain s295c)-infected owl monkey kidney monolayer cells. four to 6 weeks later, the lymphocytes showed increased clumping and cell growth and the ability to grow in the absence of il-2. control lymphocyte cultures not exposed to hvs eventually died out at approximately 6-8 weeks, even in the presence of ... | 1984 | 6087333 |
| interaction of n-methyl-n'-nitro-n-nitrosoguanidine (mnng) with owl monkey kidney cells in enhancing the yields of herpesvirus saimiri (hvs) and its antigens. | pre- and posttreatment with n-methyl-n'-nitro-nitrosoguanidine (mnng) of owl monkey kidney (omk) cells infected with herpesvirus saimiri (hvs) resulted in one to three logs higher yields of virus, depending upon the dose of mnng. a higher percentage of cells also showed hvs early antigen (ea) and late antigen (la) by immunofluorescence when omk cells infected with hvs were fed with medium containing mnng. the high yields of hvs were also observed by electron microscopy. mnng did not induce hvs-e ... | 1984 | 6087360 |
| the antiviral spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine. | the antiviral activity spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) is not restricted to herpes simplex virus type 1 (hsv-1) and varicella-zoster virus (vzv) but also encompasses several other herpesviruses such as suid herpesvirus type 1 (shv-1), bovid herpesvirus type 1 (bhv-1), simian varicella virus (svv), herpesvirus saimiri, herpesvirus platyrrhinae, and the baculovirus trichoplusia ni multiple nuclear polyhedrosis virus. other herpesviruses such as herpes simplex virus type 2, ... | 1984 | 6092320 |
| mapping of rna transcribed from a region of the herpesvirus saimiri genome required for oncogenicity. | a region of the herpesvirus saimiri genome that is not essential for replication of the virus has recently been shown to be required for its oncogenicity in new world primates. we have examined the rnas derived from this region of the genome in permissively infected cells. two polyadenylated rnas, of 4.9 and 2.3 kilobases, were the major species coded for by this region of the genome. these two rnas, as well as a much less abundant rna of 6.5 kilobases, were specifically altered in two different ... | 1984 | 6092674 |
| isolation and characterization of monoclonal antibodies to structural and nonstructural herpesvirus saimiri proteins. | an improved screening procedure was applied to identify hybridomas secreting antibodies to herpesvirus saimiri-specified polypeptides among the products of fusions between sp2/0 myeloma cells and spleen cells from mice immunized with purified virus particles or virus-specific dna-binding proteins. twenty-four monoclonal antibodies were isolated with specificities for 13 different virus-specified polypeptides (or complexes of polypeptides), including the major capsid protein of the virus (150k), ... | 1984 | 6092717 |
| classification of herpesvirus saimiri into three groups based on extreme variation in a dna region required for oncogenicity. | the leftmost 7 kilobase pairs of unique sequence l-dna of herpesvirus saimiri was found to be highly variable among different strains as determined by restriction endonuclease analysis and blot hybridization. this region in one group of viruses (group a) showed only very weak hybridization with the dna of two other groups. similarly, a fragment of group b hybridized to dna of its own group much more strongly than to group a. no homology was detected within a 1.2-kilobase-pair region between stra ... | 1984 | 6092721 |
| comparative studies of interferon and three antiviral agents on neurotropic and oncogenic herpesviruses. | the antiviral effects of four compounds, phosphonoacetate, phosphonoformate, acycloguanosine, and purified human lymphoblastic interferon, were tested against two neurotropic herpesviruses, herpesvirus platyrrhine and herpes simplex virus, and two oncogenic herpesviruses, herpesvirus saimiri and herpesvirus ateles. all four compounds induced different degrees of inhibition of these herpesviruses. phosphonoacetate and phosphonoformate at concentrations of 50 micrograms/ml or greater showed powerf ... | 1980 | 6160810 |
| epstein-barr virus and herpesvirus saimiri: sensitivity to interferons and interferon inducers. | the in vitro sensitivity of oncogenic herpesviruses, epstein-barr virus (ebv), and herpesvirus saimiri (hvs) to human interferon produced by normal human leukocytes (le), lymphoblastoid cell lines (lyi), and diploid fibroblasts (fi) was studied. four virus strains were used: hvs s295c, the highly oncogenic hvs s396-o, the transforming b95-8 strain of ebv, and the nontransforming p3hr1 strain of ebv. all interferons were active when applied to the cells after absorption of hvs and p3hr1-ebv, alth ... | 1981 | 6165852 |
| specifically unmethylated cytidylic-guanylate sites in herpesvirus saimiri dna in tumor cells. | the restriction endonucleases mspi (ccgg), hpaii (ccgg), fnudii (cgcg), and haeiii (ggcc) were used to study the methylation of herpesvirus saimiri dna in tumor cells taken directly from tumor-bearing animals. no evidence was found for methylation of the 5' terminal c in the sequence ccgg or of the internal c in the sequence ggcc, but extensive methylation of cg was detected. fifteen hpaii sites and 17 fnudii sites were detected in the unique dna region of the h. saimiri strain used. twenty-eigh ... | 1982 | 6180177 |
| analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus. | a new type of defective parvovirus, tentatively designated as adeno-associated virus type 5 (aav-5), is characterized as far as its proteins, its helper dependence, and its seroepidemiology are concerned. the protein analysis of aav-5 in polyacrylamide gels demonstrated the presence of three structural polypeptides, corresponding to vp 1, vp 2, and vp 3 of other aav types. the preparation of monoclonal antibodies against aav-5 permitted the analysis of viral structural antigen expression by usin ... | 1984 | 6200995 |
| lymphokines produced by herpesvirus-transformed marmoset monkey lymphoid cell lines. i. characterization of a constitutively produced interferon. | conditioned media from cultures of marmoset monkey t-lymphoid cell lines transformed by herpesvirus saimiri or herpesvirus ateles were found to contain interferon (ifn) activity. titers between individual cell lines varied by a factor of 100; large amounts (up to 10(5) units/ml, assayed on human cells) were produced in one of the cell lines. ifn production was enhanced by the diterpene tumor promoters, tpa and mezerein, but not by classical t-cell mitogens. the ifn resembles human ifn-gamma by t ... | 1984 | 6206645 |
| cloning of herpesvirus saimiri dna fragments representing the entire l-region of the genome. | purified particles of herpesvirus saimiri, a potent tumor-eliciting virus of primates, contain genomic dna molecules (145-170 kb) consisting of a unique l-dna region (112 kb) which is flanked by variable stretches of repetitive sequences (h-dna). restriction fragments representing the entire l-dna of h. saimiri strain no. 11 were cloned in plasmid and bacteriophage vectors. the internal fragments of l-dna generated by the enzymes ecori and kpni were inserted into plasmid pacyc184, cosmid pjc81, ... | 1983 | 6229454 |
| rna from unique and repetitive dna sequences of herpesvirus saimiri. | | 1980 | 6243199 |
| antiviral cell-mediated immune responses and effect of chromosome polymorphism in herpesvirus saimiri-infected monkeys. | herpesvirus saimiri is a horizontally transmitted virus of squirrel monkeys (saimiri sciureus) which, when inoculated into owl monkeys (aotus trivirgatus), induces fatal malignant lymphoma in 75 to 80% of the animals. previous immunological and virological studies have not indicated why 20 to 25% of inoculated owl monkeys develop a chronic, disease-free h. saimiri infection. in the present study, we examined two parameters to explain why certain owl monkeys are resistant to h. saimiri-induced di ... | 1980 | 6247277 |
| herpesvirus saimiri-induced malignant lymphoma in inbred strain iii/j rabbits (oryctolagus cuniculus). | four young strain iii/j rabbits of both sexes were inoculated with a single dose of prototype partially purified herpesvirus saimiri (hvs) via iv and im routes. all inoculated animals had enlarged lymph nodes, and significant levels of antibodies to hvs early, late, and membrane antigens were detectable during the infection. the animals died or were killed and hvs was isolated from the peripheral blood mononuclear cells and the various lymph nodes but not from the kidney. microscopic examination ... | 1980 | 6260812 |
| proteins of herpesvirus saimiri: identification of two virus polypeptides released into the culture medium of productively infected cells. | two virus-induced polypeptides are preferentially released into the culture medium of owl monkey kidney cells productively infected with herpesvirus saimiri (hvs). these polypeptides have apparent mol. wt. of 160 x 10(3) and 28 x 10(3) and are the major virus-induced polypeptides in a membrane fraction from infected cells. both these excreted polypeptides co-migrate with virus structural proteins and are selectively released from enveloped virus particles by treatment with nonidet-p40. | 1980 | 6262446 |
| herpesvirus-transformed cytotoxic t-cell lines. | investigations of cellular cytotoxicity of the immune system are hampered by the lack of continuously growing, transformed cell lines which express a cytotoxic potential. here we describe cytotoxic cell lines from the cotton-topped marmoset monkey, transformed by herpesvirus ateles (hva) or herpesvirus saimiri (hvs), which can kill certain target cells in a short-term in vitro test. hva/hvs-transformed cells have earlier been classified as belonging to the t-cell lineage in contrast to epstein-b ... | 1981 | 6262648 |
| infection of multiple t-cell subsets and changes in lymphocyte functions associated with herpesvirus saimiri infection of owl monkeys. | we examined the association of herpesvirus saimiri with lymphocyte subsets and the functional integrity and distribution of these populations in owl monkeys with chronic, disease-free infections, in uninoculated, control animals, and in one monkey with h. saimiri-induced lymphoma. the lymphocyte subpopulations examined included total t cells, t cells with receptors for the fc portion of immunoglobulin g (the tgamma cells), t cells lacking this receptor (the tgamma(-) population), and non-t cells ... | 1981 | 6265360 |
| herpesvirus saimiri: protective effect of attenuated strain against lymphoma induction. | an attenuated strain of herpesvirus saimiri (a-hvs) induced a persistent, latent infection in common marmosets. virus was recovered from circulating lymphocytes of infected animals and antibody response to virus-specified antigens was demonstrated over 3-12 months pi. a-hvs-infected common marmosets and animals without previous a-hvs exposure were challenged with oncogenic hvs (125-825 pfu); marmosets without a-hvs infection developed malignant lymphoproliferative disease and died 3 weeks pi whe ... | 1980 | 6265382 |
| herpesvirus saimiri dna in tumor cells--deleted sequences and sequence rearrangements. | herpesvirus saimiri dna in continuous lymphoblastoid cell lines obtained from viral induced tumors in marmosets has been analyzed by gel electrophoresis of restricted dna. southern transfer to nitrocellulose filters, and hybridization to 32p-labeled viral dna or dna fragments. the viral dna fragments ecori-g, -h, -d, and -i, kpni-a, and bamhi-d and -e were not detected in southern transfers of dna from the nonproducing 1670 cell line. for each restriction endonuclease, a new fragment appeared, c ... | 1981 | 6268839 |
| retinoid-induced growth inhibition of herpesvirus-transformed marmoset lymphoblastoid cell lines. | the in vitro proliferation of herpesvirus-transformed marmoset lymphoblastoid cell lines (lcl) was inhibited by retinoic acid and retinyl acetate. both herpesvirus-ateles-and herpesvirus-saimiri-transformed lcl with t-cell characteristics and epstein-barr-transformed lcl with b-cell markers were more sensitive to retinoic acid than to retinyl acetate. inhibition of lcl proliferation was dependent on retinoid concentration and became apparent only after 3-4 days of exposure. in vitro assays for m ... | 1981 | 6272171 |
| herpesvirus ateles and herpesvirus saimiri transform marmoset t cells into continuously proliferating cell lines that can mediate natural killer cell-like cytotoxicity. | herpesvirus ateles (hva) and herpesvirus saimiri (hvs) have the capacity to transform cotton-topped marmoset t lymphocytes into continuously proliferating cell lines that retain some functions associated with cell-mediated immunity. in the present paper, we demonstrate that hva/hvs-transformed t cell lines are cytotoxic in a short-term 51cr release assay and that this killing resembles killing by marmoset natural killer (nk) cells. the relationship between nk cells and hva/hvs-transformed killer ... | 1981 | 6273869 |
| episomal and nonepisomal herpesvirus dna in lymphoid tumor cell lines. | tumor cell lines derived from herpesvirus saimiri (h. saimiri)- and herpesvirus ateles (h. ateles)-induced lymphomas of new world primates and rabbits contain multiple copies of viral genomes. partial denaturation mapping and blot hybridizations of episomal dna from lymphoid tumor cell line no. 1670 showed that a 12.5md-fragment is missing which represents the ecori d- and h-fragments of virion l-dna. however, the missing piece can be demonstrated in total cellular dna by reassociation kinetics, ... | 1981 | 6274746 |
| comparison of thymidine kinase activities indiced in cells productively infected with herpesvirus saimiri and herpes simplex virus. | the replication of unselected strains of herpesvirus saimiri (hvs) was sensitive to bromodeoxyuridine and bromovinyldeoxyuridine (bvdu) but insensitive to acycloguanosine (acg), in contrast to the growth of herpes simplex virus (hsv) which was sensitive to all three analogues. mutants of hvs resistant to bromodeoxyuridine and bvdu could be selected by growth in the presence of these inhibitors. productive infections of owl monkey kidney or vero cell cultures by unselected strains of hvs resulted ... | 1982 | 6278057 |
| herpesvirus saimiri strain variability. | herpesvirus saimiri was isolated from 22 squirrel monkeys by cocultivation of peripheral lymphocytes with permissive owl monkey kidney monolayer cells. comparison of virion dna fragments produced from restriction endonuclease digestion was used as a sensitive measure of strain variability. although all isolates contained similarities and common features, 19 of the 22 were readily distinguished. three of the isolates, however, were indistinguishable and possibly were related epidemiologically. di ... | 1982 | 6287011 |
| proteins specified by herpesvirus saimiri: purification and properties of a single polypeptide which elicits virus-neutralizing antibody. | a virus-specified polypeptide of 160,000 mol. wt. (160k) was purified more than 1000-fold from the soluble proteins present in the culture medium of cells productively infected with herpesvirus saimiri (hvs). purified preparations of the 160k polypeptide gave rise to high titre precipitating and neutralizing antibodies in immunized rabbits, which cross-neutralized independent isolates of hvs (neutralization rate constants, k, of between 3.8 and 4.8) and specifically precipitated the 160k polypep ... | 1982 | 6292340 |
| structure of nonintegrated, circular herpesvirus saimiri and herpesvirus ateles genomes in tumor cell lines and in vitro-transformed cells. | nonintegrated, circular dna molecules of herpesvirus saimiri and herpesvirus ateles were found in five lymphoid cell lines originating from tumor tissues or established by in vitro immortalization of t lymphocytes. the arrangement of unique (l) and repetitive (h) dna sequences in circular viral genomes was analyzed by partial denaturation mapping followed by visualization with an electron microscope. three types of circular viral dna structures were found. (i) the virus-producing cell line rlc, ... | 1982 | 6292492 |
| variations in the immune response to herpesvirus saimiri in squirrel and rhesus monkeys. | humoral and cell-mediated immunity (cmi) to herpesvirus saimiri (hvs), an oncogenic lymphotropic herpesvirus, was studied in squirrel and rhesus monkeys. natural antibody to hvs was found in five of six squirrel monkeys but there was no evidence of specific cmi directed against hvs. rhesus monkeys did not show natural antibody or cmi against hvs antigens. immunization with hvs, however, produced both antibody and specific cmi in the rhesus monkeys, but no cmi developed in the squirrel monkeys. t ... | 1982 | 6293754 |
| identification of normal and transformed lymphocyte subsets of nonhuman primates with monoclonal antibodies to human lymphocytes. | monoclonal antibodies (mab) that detect specific lymphocyte subsets of man were tested for their reactivity with peripheral blood lymphocytes (pbl) and established cell lines of owl monkeys (aotus trivirgatus) and marmosets (saguinus spp). results of these studies showed that certain determinants were conserved and that the pattern of reactivity observed in one genus (or even species) could not be used to predict the reactivity in another. on owl monkey pbl, reactivity equivalent to that on huma ... | 1983 | 6296233 |
| proteins specified by herpesvirus saimiri: identification and properties of virus-specific polypeptides in productively infected cells. | the synthesis and accumulation of more than 30 virus-induced polypeptides was detected after infection of permissive primate cells with high multiplicities of herpesvirus saimiri. these virus-induced polypeptides had apparent mol. wt. of from 12,000 (12k) to 250k and differed in molar abundance by up to two orders of magnitude. the majority of virus-induced polypeptides satisfied multiple criteria for their virus specificity. polypeptides of 110k, 76k, 51k and 31k to 29k were synthesized at maxi ... | 1983 | 6296297 |
| herpesvirus saimiri-induced proteins in lytically infected cells. i. time-ordered synthesis. | the addition of tpa (phorbol-12-myristate-13-acetate) to cultures during the lytic infection with herpesvirus saimiri led to an enhanced and accelerated production of polypeptides induced by h. saimiri and to a rapid shut-down of host cell protein synthesis and allowed a detailed analysis of the protein patterns. analysis of sequential protein synthesis in owl monkey kidney cells lytically infected with h. saimiri 11 permitted the identification of 31 virus-induced polypeptides. the use of the a ... | 1983 | 6296304 |
| natural killer cells in relation to herpesvirus saimiri-induced disease course and manifestations in owl monkeys. | infection of owl monkeys with herpesvirus saimiri (hvs) results in disease courses ranging from chronic infections to fatal cancers. malignant disease manifestations include the appearance of transformed t-cells, suppressor t-cells, and virus-carrying cells in the peripheral circulation. the reasons for the variability in disease course remain unknown. this study examined natural killer (nk) functions in relation to disease manifestations following hvs infection. the results from lymphocyte frac ... | 1983 | 6296516 |
| identification of a subset of herpesvirus saimiri polypeptides synthesized in the absence of virus dna replication. | the effects of phosphonoacetic acid on the synthesis of herpesvirus saimiri-specific polypeptides in productively infected cells were examined. at concentrations that inhibited virus dna synthesis (greater than or equal to 150 micrograms/ml), phosphonoacetic acid prevented the synthesis of the majority of virus-specific polypeptides while allowing the synthesis of a subset of virus proteins (i.e., 110,000 [110k], 76k, 72k, 51k, 48k, 29k, 24k, and 20k or 21k) and the protracted synthesis of host- ... | 1983 | 6298461 |
| characterization of herpesvirus saimiri and herpesvirus ateles structural proteins. | the structural proteins of herpesvirus saimiri strains 11 and 11 att and of herpesvirus ateles strains 73 and 810 were characterized by electrophoresis in sds-polyacrylamide gels. for h. saimiri 21 virus structural proteins could be identified with molecular weights ranging from 28,000 to 210,000 da. for h. ateles 810 and h. ateles 73, 20 polypeptides were characterized. using lactoperoxidase for iodination of surface proteins and immunoprecipitation, 5 polypeptides could be identified as envelo ... | 1983 | 6299009 |
| failure of herpesvirus saimiri to enhance atherogenesis in owl monkeys (aotus trivirgatus). | a retrospective study was performed to evaluate the potential of a mammalian oncogenic virus, herpesvirus saimiri, to cause atherosclerosis in owl monkeys (aotus trivirgatus). this was undertaken since an avian oncogenic herpesvirus, marek's disease virus, does so in chickens. data from earlier studies were reviewed and 3 groups of animals were selected. these included 23 animals infected with herpesvirus that died an average of 156 days later with malignant lymphoma; 11 infected an average of 2 ... | 1983 | 6301514 |
| evidence for a herpesvirus saimiri-specified dna polymerase activity which is aphidicolin-resistant and phosphonoacetate-sensitive. | phosphonoacetic acid (paa) effectively inhibited herpesvirus saimiri (hvs) replication and the onset of virus dna synthesis. a paa-resistant (pr) mutant of hvs was isolated which plaqued efficiently in the presence of concentrations of paa sufficient to reduce the growth of wild-type virus to less than 0.02% of control values. in contrast, virus growth and dna synthesis in cells infected with unselected strains of herpesvirus saimiri was highly resistant to concentrations of aphidicolin, an inhi ... | 1983 | 6302206 |
| immunoprecipitation as a tool for studying humoral immunity of natural and experimental hosts of herpesvirus saimiri. | herpesvirus saimiri strain 11 and attenuated h. saimiri strain 11 proteins synthesized during the lytic cycle of virus replication were used for immunoprecipitation with various sera from natural (saimiri sciureus) and experimental (saguinus nigricollis, saguinus fuscicollis, aotus trivirgatus, new zealand white rabbits) hosts. the analysis of the precipitates separated in sodium dodecyl sulfate:polyacrylamide gels revealed that in tumor-developing animals a specific set of viral polypeptides we ... | 1983 | 6303583 |
| characterization of proteins induced by herpesvirus saimiri: comparative immunoprecipitation and analysis of glycosylation. | | 1983 | 6305800 |
| structural proteins of herpesvirus saimiri. | herpesvirus saimiri particles were purified from productively infected owl monkey kidney cell cultures, and the virion polypeptides were analyzed by polyacrylamide gel electrophoresis. a total of 21 predominant proteins were found in lysates of h. saimiri 11 particles by coomassie blue staining or by [35s]methionine labeling and autoradiography; all proteins were between 160,000 and 12,000 daltons in size. they are most probably virion constituents, as most of them were precipitated by immune se ... | 1983 | 6312078 |
| virus-specific transcription in a herpesvirus saimiri-transformed lymphoid tumor cell line. | herpesvirus saimiri-transformed lymphoid tumor cell lines contain nonintegrated covalently closed circular viral dna molecules in high multiplicity. one of those cell lines, 1670, carries large viral dna circles (202 kilobase pairs) with two stretches of repetitive dna (70.8% g + c) that are interspersed between two segments of unique dna (36% g + c). since it was not known if there is any viral gene expression in h. saimiri-transformed cells, we initiated a study of transcription in cell line 1 ... | 1983 | 6312095 |
| dna-binding proteins specified by herpesvirus saimiri. | herpesvirus saimiri-specific proteins from the nuclear fractions of productively infected owl monkey kidney cells were dissociated from virus and host dna by treatment with 2 m-nacl or separation on urografin density gradients. empty virus capsids remained intact and could be separated from major non-structural proteins (110k, 51k and 48k) and from a subset of structural proteins (130k, 29k and 12k), either by urografin gradient sedimentation or differential centrifugation. the dna in such solub ... | 1983 | 6319554 |
| construction of replication-competent herpesvirus saimiri deletion mutants. | dna fragments derived from the left end of herpesvirus saimiri 11 l-dna were cloned in escherichia coli by using vector pbr322. deletions were introduced within a cloned 7.4-kilobase-pair sequence by using restriction endonucleases that cut once or twice within this sequence. permissive owl monkey kidney-cultured cells were transfected with parental strain 11 viral dna plus cloned dna with specific sequences deleted. by screening the progeny of these transfections with a limiting-dilution spot h ... | 1984 | 6319734 |
| herpesvirus saimiri dna in a lymphoid cell line established by in vitro transformation. | a lymphoid t-cell line (h1591) was established by infecting peripheral blood mononuclear cells from a cotton top marmoset with herpesvirus saimiri omi. analysis of these in vitro-immortalized cells revealed nonintegrated, covalently closed circular viral dna molecules in high multiplicities with substantial rearrangements and large deletions in their l-dna (unique) regions. one subline, designated h1591 er, contained circular viral dna with one stretch of h-dna (repetitive) and one of l-dna; the ... | 1984 | 6321774 |
| detection of circular and linear herpesvirus dna molecules in mammalian cells by gel electrophoresis. | a simple gel technique is described for the detection of large, covalently closed, circular dna molecules in eucaryotic cells. the procedure is based on the electrophoretic technique of eckhardt (t. eckhardt, plasmid 1:584-588, 1978) for detecting bacterial plasmids and has been modified for the detection of circular and linear extrachromosomal herpesvirus genomes in mammalian cells. gentle lysis of suspended cells in the well of an agarose gel followed by high-voltage electrophoresis allows sep ... | 1984 | 6321792 |
| deletion of dna sequence in a nononcogenic variant of herpesvirus saimiri. | the 110-kilobase-pair stretch of unique sequence dna of herpesvirus saimiri is flanked by highly repetitive dna. detailed restriction endonuclease mapping has localized the left junction of repetitive and unique dna to a 100-base-pair region. h. saimiri 11att, a replication competent nononcogenic variant of strain 11, has a deletion of 2.3 kilobase pairs of sequence information that spans this left junction of repetitive and unique dna. | 1984 | 6323769 |
| a cytotoxic t-lymphocyte line propagated from a rabbit infected with sheep associated malignant catarrhal fever. | the propagation of a cell line from a rabbit affected with the sheep associated form of malignant catarrhal fever is described. immunological and morphological characteristics of the cell indicated that it was a t-lymphocyte and the presence of electron dense cytoplasmic granules suggested that the cell could be further classified as a large granular lymphocyte. the cell line required a feeder layer and was cytotoxic to both primary cell cultures and cell lines, a characteristic of large granula ... | 1983 | 6601284 |
| interaction of herpesvirus ateles and hepesvirus saimiri with primate lymphocytes. i. selective adsorption of virus by lymphoid cells. | a semiquantitative infectious bioassay, described by sairenji and hinuma for the measurement of epstein-barr virus (ebv) receptors, was adapted to study the adsorption of the lymphotropic new world herpesvirus ateles (hva) to established lymphoid lines and lymphocytes of human versus marmoset origin. all marmoset t-cell lines [hva- or hvs (herpesvirus saimiri)-carrying] had a high adsorbing capacity. ebv-carrying marmoset or human b-cell lines, one ebv-negative human b-lymphoma line, and one hum ... | 1980 | 6766914 |
| a marmoset t lymphoma which functions as a human amplifier t cell. | a long-term in vitro grown t cell line derived from a cotton-topped marmoset (saguinus oedipus) infected with herpesvirus saimiri was fond to share surface antigens with human amplifier t cells and to augment the capacity of human b cells to secrete immunoglobulin. this is the first demonstration of t/b collaboration across such a large phylogenetic barrier and might have interesting implications for understanding the nature of molecular interactions mediating cell/cell cooperation. | 1981 | 6801144 |
| adult t-cell leukemia: antigen in an atl cell line and detection of antibodies to the antigen in human sera. | indirect immunofluorescence of certain human sera demonstrated an antigen(s) in the cytoplasm of 1--5% of the cells of a t-cell line, mt-1, from a patient with adult t-cell leukemia (atl), which is endemic in southwestern japan. the antigen was not detected in other human lymphoid cell lines, including six t-cell lines, seven b-cell lines, and four non-t non-b cell lines. the antigen did not show cross antigenicity with that of herpesviruses, including epstein--barr virus, herpes simplex virus, ... | 1981 | 7031654 |
| syncytium formation in aged umbilical cord blood macrophages. attempts to demonstrate an infectious etiology. | about 50% of cultures of mononuclear cells from human umbilical cord blood kept for more than 6 weeks in tissue culture reveal syncytium formation of adherent cells involving varying percentages of cells. syncytium formation usually occurs focally producing plaques of multinucleated giant cells followed by cell degeneration. occasionally, plaques of giant cell formation of adherent cells were also noted in macrophage cultures obtained from healthy adult blood donors. attempts to transfer cytopat ... | 1982 | 7099112 |
| downregulation of lck-mediated signal transduction by tip of herpesvirus saimiri. | a protein, called tip, of herpesvirus saimiri associates with lck in transformed t cells. to investigate the effects of complex formation on cellular signal transduction, we constructed human jurkat-t-cell lines expressing tip. the expression of tip in jurkat-t cells dramatically suppressed cellular tyrosine phosphorylation and surface expression of lymphocyte antigens. the expression of tip also blocked the induction of tyrosine phosphorylation by anti-cd3 stimulation. the expression of tip in ... | 1995 | 7494293 |
| immortalization of human t cells expressing t-cell receptor gamma delta by herpesvirus saimiri. | herpesvirus saimiri (hvs) has recently been shown to immortalize human cd4+ and cd8+ t cells expressing t-cell receptor alpha beta (tcr-alpha beta) with the maintenance of their original phenotypes and functional properties. however, the immortalization of human t cells expressing tcr-gamma delta by hvs has not been successful. here we report that hvs can also infect and immortalize human t cells expressing tcr-gamma delta. two human tcr-gamma delta+ t-cell clones, which continuously proliferate ... | 1995 | 7494332 |
| activation of a myelin basic protein-specific human t cell clone by antigen-presenting cells from rhesus monkeys. | this study addresses the capacity of peripheral blood mononuclear cells (pbmc) from rhesus monkeys (macaca mulatta) to present myelin basic protein (mbp), a candidate auto-antigen for multiple sclerosis, to mbp-specific human cd4+ t cell clones. mhc-restriction of the human t cell clones was determined with hla-dr-transfected l cells, and epitope specificity was established with a panel of overlapping 20-mer peptides. the mhc-dr region of the rhesus monkeys (mamu) was characterized serologically ... | 1995 | 7495756 |
| human il-17: a novel cytokine derived from t cells. | a cdna encoding human il-17 (hil-17) was cloned from a cd4+ t cell library. the predicted 155-amino acids sequence contains an n-terminal signal peptide and exhibits 72% amino acid identity with hvs13, an open reading frame from a t-lymphotropic herpesvirus saimiri, and 63% with murine ctla8. high levels of hil-17 were induced from primary peripheral blood cd4+ t cells upon stimulation. when expressed in cv1/ebna cells, recombinant hil-17 was secreted in both glycosylated and nonglycosylated for ... | 1995 | 7499828 |
| transformation of human t-cell clones by herpesvirus saimiri: intact antigen recognition by autonomously growing myelin basic protein-specific t cells. | herpesvirus saimiri has recently been shown to immortalize human t cells. it was unknown, however, whether herpesvirus saimiri transformation affects t-cell receptor (tcr) expression and signal transduction. in the present study, we have transformed cd4+ human t-cell clones specific for human myelin basic protein. the transformed t cells were grown in interleukin 2 and divided in the absence of antigen and antigen-presenting cells. they retained the membrane phenotype of activated t cells and se ... | 1993 | 7504291 |
| inhibition of complement-mediated cytolysis by the terminal complement inhibitor of herpesvirus saimiri. | herpesvirus saimiri (hvs) is a lymphotropic herpesvirus that induces t-cell transformation in vitro and causes lymphomas and leukemias in new world primates other than its natural host, the squirrel monkey. nucleotide sequence analysis of the hvs genome revealed two open reading frames with significant homology to genes for human complement regulatory molecules. one of these genes encodes a predicted protein (designated hvscd59) with 48% amino acid sequence identity to the human terminal complem ... | 1994 | 7507185 |
| polyclonal b cell activation induced by herpesvirus saimiri-transformed human cd4+ t cell clones. role for membrane tnf-alpha/tnf-alpha receptors and cd2/cd58 interactions. | we have shown that in vitro infection herpesvirus saimiri (hvs) can transform human cd4+ t cell clones with defined th1 or th2 cytokine profiles to continuous growth. we report here that transformation with hvs enabled both th1 and th2 clones to stimulate proliferation and ig production by autologous or allogeneic b cells in the absence of stimulants. the polyclonal b cell-activating property of hvs-transformed clones was not related to free virus or soluble cytokines, but rather was dependent o ... | 1994 | 7525717 |
| a cytotoxic cd40/p55 tumor necrosis factor receptor hybrid detects cd40 ligand on herpesvirus saimiri-transformed t cells. | the b cell activation molecule cd40 and the p55 tumor necrosis factor receptor (p55tnfr) belong to the same family of structurally conserved proteins. we constructed a chimeric receptor consisting of the cd40 extracellular and transmembrane domains and the p55tnfr intracellular domain. this receptor hybrid retained the biological activity and the ligand specificity of the respective wild-type receptor domains. thus it exerted a marked cytotoxic effect in three different transfected cell lines af ... | 1995 | 7531151 |
| identification of lck-binding elements in tip of herpesvirus saimiri. | a protein called tip (tyrosine kinase interacting protein) of herpesvirus saimiri associates with lck in virus-transformed human t cells and is an in vitro substrate for lck kinase. mutational analyses of a gst-tip fusion protein revealed that binding to lck requires putative sh3 binding sequences and a sequence homologous to the carboxyl terminus of src-related kinases. these sequences are referred to as sh3-binding (sh3b) and c-terminal src-related kinase homology (cskh) elements. peptide frag ... | 1995 | 7544793 |
| immortalization of human t cell clones by herpesvirus saimiri. signal transduction analysis reveals functional cd3, cd4, and il-2 receptors. | investigation of human activated t cells has been complicated by the need for periodic restimulation with ag/mitogen and accessory cells and by the limited life span of most human t cell clones. to overcome these problems, we have transformed established human t cell clones to permanent growth with herpesvirus saimiri, a lymphoma-inducing virus of nonhuman primates. three human cd4+ t cell clones were investigated in detail. they have been growing in the presence of exogenous il-2 but without re ... | 1993 | 7687626 |
| induction of a herpesvirus saimiri small rna au binding factor (aubf70) activity and lymphokine mrnas by t cell mitogens. | herpesvirus saimiri (h. saimiri) can transform t lymphocytes and cause lymphoid tumors in rabbits and new world monkeys. h. saimiri-immortalized t cells express il-2 and il-4. the putative oncogenes of a group c strain of h. saimiri have been mapped to a region of the unique l-dna which includes genes encoding four u-like small nuclear rnas (hsur1-hsur4). jurkat t cells express a 70 kd rna binding factor (aubf70) which binds hsur2. here we examined aubf70 expression in resting and mitogen-stimul ... | 1995 | 7733817 |
| identification and analysis of an alcelaphine herpesvirus 1 (ahv-1) cdna clone expressing a fusion protein recognized by ahv-1-neutralizing antisera. | rabbit antiserum to psoralen-inactivated alcelaphine herpesvirus 1 (ahv-1) virions was shown to react specifically with ahv-1-infected cells by indirect immunofluorescence. western blot analysis using this antiserum identified a 15-kd virion protein that was also detected in infected-cell proteins between 12 and 144 h p.i., and a 37-kd protein present in infected cells between 24 and 120 h p.i. a cdna library was constructed using mrna obtained from ahv-1-infected fetal mouflon sheep kidney (fms ... | 1995 | 7733826 |
| the complement control protein homolog of herpesvirus saimiri regulates serum complement by inhibiting c3 convertase activity. | the herpesvirus saimiri genome encodes a complement control protein homolog (ccph). stable mammalian cell transfectants expressing a recombinant transmembrane form of ccph (mccph) or a 5'flag epitope-tagged mccph (5'flagmccph) conferred resistance to complement-mediated cell damage by inhibiting the lytic activity of human serum complement. the function of ccph was further defined by showing that the mccph and the 5'flagmccph transfectants inhibited c3 convertase activity and effectively reduced ... | 1995 | 7745740 |
| expression of il-2 and il-4 in t lymphocytes transformed by herpesvirus saimiri. | herpesvirus saimiri (h. saimiri) is a highly oncogenic lymphotropic herpesvirus which can immortalize t lymphocytes and cause tumors in rabbits and new world monkeys. t cells infected with strain 484-77 of group c express four viral u-like small rnas (hsur1-4) and a 1.2-kb mrna which encodes open reading frames orf-1 and orf-2. orf-1 encodes a collagen-like oncoprotein. deletion mutation analysis showed that orf-1 and orf-2 are essential for il-2 independent growth of human t cells infected with ... | 1995 | 7747414 |
| the dna sequence of human herpesvirus-6: structure, coding content, and genome evolution. | the complete dna sequence was determined for strain u1102 of human herpesvirus-6, a cd4+ t-lymphotropic virus with disease associations in immunodeficient settings and a possible complicating factor in aids. the genome is 159,321 bp in size, has a base composition of 43% g + c, and contains 119 open reading frames. the overall structure is 143 kb bounded by 8 kb of direct repeats, drl (left) and drr (right), containing 0.35 kb of terminal and junctional arrays of human telomere-like simple repea ... | 1995 | 7747482 |
| identification of a highly specific surface marker of t-cell acute lymphoblastic leukemia and neuroblastoma as a new member of the transmembrane 4 superfamily. | five monoclonal antibodies detected a surface antigen expressed exclusively on t-cell acute lymphoblastic leukemia (t-all) in a panel of 45 human hematopoietic cell lines, including t-cell lines derived from adult t-cell leukemia and those established by immortalization with human t-cell leukemia virus type 1 or herpesvirus saimiri. peripheral blood mononuclear cells, including fresh and activated t cells, were also completely devoid of this antigen. we designated this antigen as talla-1 (from t ... | 1995 | 7768645 |
| a herpesvirus saimiri protein required for interleukin-2 independence is associated with membranes of transformed t cells. | a region of the herpesvirus saimiri genome encoding an mrna with two open reading frames (orfs) has been identified to be essential for transformation of t cells. deletion of either orf resulted in the loss of transforming ability. orf-1 has been shown to code for a collagen-like oncoprotein. this study shows for the first time that the bicistronic mrna can translate a 32-kda protein from orf-2. polyclonal serum to orf-2 was generated by using a glutathione fusion protein. using this antiserum, ... | 1995 | 7769710 |
| the dna sequence of equine herpesvirus 2. | the complete dna sequence of equine herpesvirus 2 (ehv-2) strain 86/67 was determined. the genome is 184,427 bp in size and has a base composition of 57.5% g + c. unusually for a herpesvirus, about a third of the sequence distributed in several large blocks appears not to encode proteins. the 79 open reading frames that were identified as probably polypeptide-coding are predicted to encode 77 distinct proteins. amino acid sequence comparisons confirmed that ehv-2 is a gamma-herpesvirus that is g ... | 1995 | 7783207 |
| glycoprotein b of bovine herpesvirus type 4: its phylogenetic relationship to gb equivalents of the herpesviruses. | in order to estimate the phylogenetic relationship of bhv-4 among the herpesviruses, we have cloned and sequenced its glycoprotein b (gb). the 2.6 kb open reading frame codes for a 874 amino acid long protein. the comparison of its deduced amino acid sequence with those of its counterparts in 19 distinct herpesviruses groups bhv-4 into the gamma-herpesvirinae. the calculation of an evolutionary tree emphasized that bhv-4 is more closely related to herpesvirus saimiri (hvs) than to epstein-barr v ... | 1994 | 7871762 |
| the product of the herpesvirus saimiri open reading frame 1 (tip) interacts with t cell-specific kinase p56lck in transformed cells. | subgroup c strains of herpesvirus saimiri, a leukemogenic virus of non-human primates, transform human t cells to permanent growth in culture. these cell retain their antigen specificity, and they are becoming widely used as a model for activated human t cells. though a variety of human cell types can be infected by h. saimiri, transformation appears to be specific for cd4+ and cd8+ t cells. our investigation of early signaling events in h. saimiri-transformed t cells revealed a novel 40-kda pho ... | 1995 | 7876245 |
| immortalization of human t cells by herpesvirus saimiri. | | 1995 | 7888066 |
| cytokine dependence of human to mouse graft-versus-host disease. | human peripheral blood leucocytes (pbl) induce chronic graft versus-host disease (gvhd) in non-conditioned severe combined immunodeficient mice. chronic gvhd was observed in such animals after transplantation of 6 x 10(7) human pbl per g body weight. however, acute xenogeneic gvhd results from grafting at least 2 x 10(7) human pbl per g body weight to heavily conditioned murine hosts. the large numbers of human pbl were thought to be required to produce above threshold amounts of certain cytokin ... | 1994 | 7913246 |
| htlv-ii non-integrated malignant lymphoma induction in japanese white rabbits following intravenous inoculation of htlv-ii-infected simian leukocyte cell line (si-iia). | lymphoma induction in rabbits by an unknown factor derived from an htlv-ii-producing simian (cynomolgus) leukocyte cell line (si-iia) is reported. thirteen of 17 male japanese white rabbits (76%) inoculated intravenously with si-iia cells developed malignant lymphoma including hodgkin-like lymphoma between 62 and 167 days after inoculation. histologically, there was extensive diffuse or nodular infiltration of either large cell type or mixed type lymphoma cells in many organs, frequently involvi ... | 1994 | 7928626 |
| virus-encoded cyclin. | herpesvirus saimiri contains an open reading frame called eclf2 with homology to the cellular type d cyclins. we now show that the eclf2 gene product is a novel virus-encoded cyclin (v-cyclin). the protein encoded by the v-cyclin gene of this oncogenic herpesvirus was found to have an apparent molecular size of 29 kda in transformed cells. v-cyclin protein was found to be associated with cdk6, a cellular cyclin-dependent kinase known to interact with cellular type d cyclins. cdk6/v-cyclin comple ... | 1994 | 7935438 |
| distinct functional domains of stp-c488 of herpesvirus saimiri. | the stp-c488 oncogene of herpesvirus saimiri has transforming activity independent of the rest of the viral genome. three distinct structural regions can be predicted from the stp-c488 sequence: an acidic amino terminal domain, a collagen domain, and a hydrophobic carboxyl-terminal domain. to study the importance and functional roles of these regions, 25 different mutant forms of stp-c488 were generated. net negative charge in the 17 amino acid amino-terminal domain was found to be important for ... | 1994 | 7941343 |
| epstein-barr virus sm protein. | the protein products of the epstein-barr virus (ebv) bmlf1 open reading frame have been characterized in the early productive cycle in b95-8 and akata cells. the sm protein derived from the spliced rna joining bslf2 to bmlf1 is much the most abundant protein. sm is a phosphoprotein in ebv-infected cells and can be phosphorylated in vitro with casein kinase ii (ckii). computer analysis of the sm protein sequence showed a c terminal section of sm to be related to genome positional homologues of fo ... | 1994 | 7975218 |
| identification of herpesvirus-like dna sequences in aids-associated kaposi's sarcoma. | representational difference analysis was used to isolate unique sequences present in more than 90 percent of kaposi's sarcoma (ks) tissues obtained from patients with acquired immunodeficiency syndrome (aids). these sequences were not present in tissue dna from non-aids patients, but were present in 15 percent of non-ks tissue dna samples from aids patients. the sequences are homologous to, but distinct from, capsid and tegument protein genes of the gammaherpesvirinae, herpesvirus saimiri and ep ... | 1994 | 7997879 |
| the molecular biology of leukocyte chemoattractant receptors. | leukocytes migrate from the blood to sites of inflammation in response to locally produced chemoattractants that activate specific cell surface receptors. the primary structures of leukocyte receptors for n-formyl peptides, c5a, platelet-activating factor, and 8 of the 18 known human chemokines (interleukin-8 and related molecules) have been deduced from cloned cdnas. all of these are seven-transmembrane-domain rhodopsin-like g protein-coupled receptors. biochemical and molecular genetic analysi ... | 1994 | 8011292 |
| herpesvirus saimiri small rna and interleukin-4 mrna auuua repeats compete for sequence-specific factors including a novel 70k protein. | a highly oncogenic strain of the lymphotropic tumour virus herpesvirus saimiri (hvs; strain 484-77) expresses four small rnas (hsur1 to 4) in high copy numbers in transformed t cells. in hsur1 and hsur2 the 5' terminal regions contain conserved auuua sequence repeats. the same auuua repeats occur in the 3' non-coding regions of growth factor, lymphokine and protooncogene mrnas, and the sequence is involved in rapid mrna degradation. we report here that by using a highly specific u.v. cross-linki ... | 1994 | 8077927 |
| molecular evolution of herpesviruses: genomic and protein sequence comparisons. | phylogenetic reconstruction of herpesvirus evolution is generally founded on amino acid sequence comparisons of specific proteins. these are relevant to the evolution of the specific gene (or set of genes), but the resulting phylogeny may vary depending on the particular sequence chosen for analysis (or comparison). in the first part of this report, we compare 13 herpesvirus genomes by using a new multidimensional methodology based on distance measures and partial orderings of dinucleotide relat ... | 1994 | 8107249 |
| expression of the collagen-like putative oncoprotein of herpesvirus saimiri in transformed t cells. | herpesvirus saimiri induces acute lymphomas and leukemias in new world primates and rabbits. previous work revealed that a highly oncogenic group c strain 484-77 encodes and expresses a bicistronic mrna in tumor-derived t cells and the first open reading frame (orf1) is highly homologous to collagen. with the aid of an antibody against a synthetic orf1 peptide, we now report that the orf1 collagen-like protein is expressed in rabbit tumor derived cell lines and in vitro transformed human and mon ... | 1993 | 8122395 |
| abnormal b cell helper activity by virus-infected human cd4+ t cells. | human cd4+ t cell clones infected in vitro with the human immunodeficiency virus (hiv), unlike their noninfected counterparts, induced both proliferation and immunoglobulin (ig) production by both autologous and allogeneic b cells through an antigen (ag)-nonspecific, mhc-unrestricted, contact-dependent mechanism. this was done apparently without expressing the cd40 ligand. interestingly, hiv-infected t cell clones, unlike their noninfected counterparts, constitutively expressed mrna for, and rel ... | 1993 | 8123798 |
| engagement of the cd4 receptor inhibits the interleukin-2-dependent proliferation of human t cells transformed by herpesvirus saimiri. | infection with herpesvirus saimiri, a tumor virus of non-human primates, transformed human cd4+ t cell clones to permanent interleukin (il)-2-dependent growth without need for restimulation with antigen and accessory cells. the il-2-dependent proliferation of these cells was dramatically inhibited by soluble anti-cd4 whole antibodies, f(ab')2 and fab fragments, and also by gp 120 of human immunodeficiency virus. the inhibition was not due to cell death and could be overcome by high concentration ... | 1994 | 8149955 |
| the analysis of polypeptides in the nuclei and cytoplasm of cells infected with murine herpesvirus 72. | twenty-six polypeptides in murine herpesvirus isolate 72 (mhv-72) were identified and the synthesis and accumulation of 21 virus-specific polypeptides in vero cells during the course of productive infection with mhv-72 were examined. five of the infected cell polypeptides accumulated within the nuclei and nine accumulated within the cytoplasm of mhv-72-infected cells. seven polypeptides were identified within the nuclei and cytoplasm in equivalent amounts. the major capsid protein was shown to h ... | 1994 | 8207392 |
| small rna expression from the oncogenic region of a highly oncogenic strain of herpesvirus saimiri. | herpesvirus saimiri induces acute lymphomas and leukemias in primates and rabbits. sequence divergence of the right end unique region of the genome classifies virus strains into three groups (a, b, and c), and previous studies have demonstrated correlation between dna grouping and oncogenicity. in order to relate different oncogenicity to the underlying molecular mechanisms, we reported earlier the expression of a bicistronic mrna from the oncogenic region in a highly oncogenic group c strain, a ... | 1994 | 8209420 |
| epithelial tumours induced by a herpesvirus oncogene in transgenic mice. | to investigate the role of herpesviral genes in tumourigenesis, transgenic mice were generated expressing stp-c, a transformation associated protein of the lymphoma inducing herpesvirus saimiri. epithelial tumours developed in the salivary gland, pancreas, thymus and liver of transgenic mice within the first weeks of life. thus, the target cells for tumour formation in the transgenic mice were surprisingly different from those of the herpesvirus from which the oncogene was derived. our results i ... | 1994 | 8302583 |