| a 15-hydroxyprostaglandin dehydrogenase specific for prostaglandin a in rabbit kidney. | examination of a soluble fraction derived from homogenates of rabbit kidney papilla revealed the existence of a 15-hydroxyprostaglandin dehydrogenase specific for a-type prostaglandins. prostaglandins of the e- and f-series were not substrates for this enzyme. in agreement with published data, the 15-hydroxyprostaglandin dehydrogenase(s) derived from the kidney cortex were found to degrade all prostaglandins examined (pge, pgf, pga) in the presence of added cofactor nad. thus it is evident that ... | 1976 | 4794 |
| the location of c-protein in rabbit skeletal muscle. | | 1976 | 4802 |
| electron microscopy of myosin molecules from muscle and non-muscle sources. | | 1976 | 4809 |
| azoaldolase photosensitivity. | | 1976 | 4845 |
| in vitro and in vivo studies of (1-sarcosine, 8-threonine) angiotensin ii. | the capacity of (1-sarcosine, 8-threonine) angiotensin ii to block the contractile and pressor effects of exogenous and endogenous angiotensin ii was examined. in isolated rabbit aorta, the pa2 value (from pa2 plots) for the analog was 8.75 +/- 0.11. both the maximum response and the slope of the dose-response curve to angiotensin ii were unchanged by the analog. in ganglion-blocked vagotomized rats, infusion of the analog produced a dose-dependent blockage of the angiotensin ii pressor effect. ... | 1976 | 4864 |
| alpha-adrenergic receptor identification by (3h)dihydroergocryptine binding. | a radioactively labeled alpha-adrenergic antagonist, 3hdihydroergocryptine, binds specifically to a site on rabbit uterine membranes. binding is rapid, reaching equilibrium in less than 17 minutes at 25 degrees c. adrenergic agonists compete for this binding site with an order of affinities identical to the pharmacological potency order of these agents as alpha-adrenergic agonists (epinephrine greater than norepinephrine greater than isoprotereonl). the (-) stereoisomers of epinephrine and norep ... | 1976 | 4894 |
| new concepts on the pathogenesis of atherosclerosis and its practical aspects. | | 1976 | 4928 |
| pharmacological action of triazolam. | | 1975 | 4945 |
| hormones, second messengers, and the mammalian spermatozoon. | | 1975 | 4967 |
| myocardial co2 buffering: role of transmembrane transport of h+ or hco3-ions. | isolated rabbit hearts were perfused with rabbit red cells suspended in ringer solution. a small volume of perfusate was recirculated for 10 min at pco2 of 33.4 +/- 0.9 or 150.8 +/- 7.5 mmhg. hypercapnia resulted in an increase in perfusate hco3- concentration that was smaller than that observed when isolated perfusate was equilibrated in vitro with the same co2 tensions (delta hco-3e = 1.6 mm, p less than 0.01). this difference is consistent with a net movement of hco3- into or h+ out of the my ... | 1976 | 4980 |
| evidence for dimethyltryptamine (dmt) as a naturally-occurring transmitter in mammalian brain. | | 1976 | 4987 |
| toxicological characteristics of ampicillin. | toxocity of ampicillin trihydrate was studied in acute and chronic experiments. it was shown that the antibiotic had low acute toxicity, did not cumulate and had no skin-irritating effect. on its inhalation in concentrations of 5 mg/m3 for 4 months, ampicillin induced allergization of albino rats, decreased their immunity. the general toxic effect of the drug was slightly pronounced. ampicillin in a concentration of 0.1 mg/m3 induced tension of the immunological reactivity of the organism. the m ... | 1975 | 5051 |
| gamma-glutamyl transpeptidase in the urine from an isolated rabbit kidney perfused with and without dmso. | | 1976 | 5095 |
| activation of the o-glucuronide of the carcinogen n-hydroxy-n-2-fluorenylacetamide by enzymatic deacetylation in vitro: formation of fluorenylamine-trna adducts. | | 1976 | 5097 |
| isolation of brain endopeptidases: influence of size and sequence of substrates structurally related to bradykinin. | two thiol-activated endopeptidases with ph optima near ph 7.5 were isolated from the supernatant fraction of rabbit brain homogenates by deae-cellulose chromatography, gel filtration and isoelectrofocusing. peptide bond hydrolysis was measured quantitatively by ion-exchange chromatography with an amino acid analyzer. brain kininase a hydrolyzes the phe5-ser6 peptide bond in bradykinin (bk), arg1-pro2-pro3-gly4-phe5-ser6-pro7-phe8-arg9. it is isoelectric near ph 5.2 and has a molecular weight of ... | 1976 | 5120 |
| metabolism of trans-stilbene in rabbits and rats. | the biotransformation of intramuscularly administered 14c-labeled trans-stilbene was investigated in both rabbits and rats. in rabbits radioactivity was excreted (78.8% in 17 days) almost exclusively in the urine. in rats, feces accounted for more than half of the total excretion (80.3% in 19 days). hydroxylated metabolites in urine and feces were identified by thin-layer chromatography and quantitated by liquid scintillation counting. they were 4-hydroxy and 4,4'-dihydroxystilbene, and the two ... | 1976 | 5260 |
| studies of the metabolism of parathion with an apparently homogeneous preparation of rabbit liver cytochrome p-450. | the metabolism of parathion has been examined by use of a reconstituted mixed-function oxidase enzyme system isolated from the livers of phenobarbital-pretreated rabbits. the cytochrome p-450 used in these studies was apparently homogeneous as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and by assay of the preparation for contaminating microsomal enzymes and enzyme activity. these studies revealed that the apparently homogeneous preparation of cytochrome p-450, in the ... | 1976 | 5264 |
| reconstituted liver microsomal enzyme system that hydroxylates drugs, other foreign compounds, and endogenous substrates. ix. the formation of a 455-nm metabolite-cytochrome p-450 complex. | the reconstituted liver microsomal hydroxylation system was used to study the formation of a metabolite-cytochrome p-450 complex absorbing maximally at 455 nm, with benzphetamine and n-hydroxyamphetamine as substrates. complex formation required the presence of nadph, substrate, nadph-cytochrome c reductase, lipid, and cytochrome p-450, indicating that metabolism of the substrate is essential. in the presence of fixed amounts of lipid and nadph-cytochrome c reductase, the rate of complex formati ... | 1976 | 5265 |
| isozymes of amp deaminase in red and white skeletal muscles. | | 1975 | 5297 |
| histamine effects on h+ permeability by isolated gastric mucosa. | this study examines the effects of histamine on mucosal permeability to acid by isolated stomach of rabbits. histamine (9 x 10(-5) m) decreased antral luminal acid loss which could not be accounted for by active h+ secretion or appearance of organic acid. histamine also increased antral electrical resistance and decreased the unidirectional luminal to serosal flux (jls) of (14)c-erythritol. histamine, theophylline, and n(6),o(2)-dibutyryl adenosine 3',5'-monophosphate did not significantly alter ... | 1976 | 5335 |
| developmental changes in the structure and kinetic properties of myosin adenosinetriphosphatase of rabbit skeletal fast muscle. | structural and functional changes in myosin of fast muscles during early post-natal development were studied to seek correlations with well-known physiological changes in the contraction rate. the findings were as follows: 1. it is known that fetal fast muscle myosin contains three kinds of light chains. it was confirmed that their molecular weights were the same as those of adult fast muscle myosin, but different from those of adult slow muscle myosin. the amount of the smallest light chain, g3 ... | 1975 | 5417 |
| control of rabbit liver fructose-1, 6-diphosphatase activity by magnesium ions. | edta at a concentration of 1 mum produced a threshold effect in the activation of purified rabbit liver fructose-1, 6-diphosphatase ec 3.1.3.11 in the presence of 5 mm mg2+ at ph 7.2. without edta, biphasic activation curves were produced by mg2+. a double-reciprocal plot of the data gave the km values corresponding to the two linear regions. they were 0.19 and 0.83 mm at ph 7.5, and 0.055 and 0.83 mm at ph 9.1. in the presence of 5mum edta a sigmoidal curve was obtained for mg2+ activation in t ... | 1975 | 5418 |
| rabbit skeletal muscle glycogen synthase. ii. enzyme phosphorylation state and effector concentrations as interacting control parameters. | the effects of several inhibitors (atp, adp, amp, udp, and p1) and activators (mg2+, glucose-6-p) of rabbit muscle glycogen synthase (udp-glucose:glycogen 4-alpha-glucosyltransferase, ec 2.4.1.11) were studied in relation to the phosphorylation state of the purified enzyme. all the modifiers had increasing effects with enzyme of increasing alkali-labile phosphate content. in experiments where combinations of effectors were present, it was apparent that (a) concentrations of modifiers in the phys ... | 1976 | 5435 |
| regulation of steady state level of phosphoenzyme and atp synthesis in sarcoplasmic reticulum vesicles during reversal of the ca2+ pump. | the role of the ca2+ concentration gradient in atp synthesis and membrane phosphorylation by pi was investigated in sarcoplasmic reticulum vesicles isolated from rabbit skeletal muscle. the pi concentration required to attain 50% of the maximal membrane phosphorylation varies significantly in the ph range of 5.5 to 4.5, the optimal being at ph 6.0. in the ph range of 6.0 to 7.0, this concentration of pi was 4- to 10-fold higher in empty vesicles than in vesicles loaded with calcium phosphate, i. ... | 1976 | 5437 |
| triglyceride, diglyceride, monoglyceride, and cholesterol ester hydrolases in chicken adipose tissue activated by adenosine 3':5'-monophosphate-dependent protein kinase. chromatographic resolution and immunochemical differentiation from lipoprotein lipase. | hormone-sensitive lipase and cholesterol ester hydrolase of chicken adipose tissue were markedly activated by adenosine 3':5'-monophosphate (camp)-dependent protein kinase (on the average, 235 to 275%; occasionally as much as 1000%). diglyceride and monoglyceride hydrolases were also activated, but to a lesser extent (60 to 87%). the activation of all four hydrolases was inhibited by protein kinase inhibitor and reversed by the addition of exogenous protein kinase. following activation by camp-d ... | 1976 | 5445 |
| affinity labeling of a previously undetected essential lysyl residue in class i fructose bisphosphate aldolase. | the affinity label n-bromoacetylethanolamine phosphate (bracnhetop) has been used previously at ph 6.5 to identify his-359 of rabbit muscle aldolase as an active site residue. we now find that the specificity of the reagent is ph-dependent. at ph 8.5, alkylation with 14c-labeled bracnhetop abolishes both fructose-1,6-p2 cleavage activity and transaldolase activity. the stoichiometry of incorporation, the kinetics of inactivation, and the protection against inactivation afforded by a competitive ... | 1976 | 5453 |
| biological inactivation of proteins by the maillard reaction. effect of mild heat on the tertiary structure of insulin. | | 1976 | 5473 |
| the binding of complement by complexes formed between a rabbit antibody and oligosaccharides of increasing size. | immune complexes formed between a homogeneous rabbit antibody to type iii pneumococcal polysaccharide and a series of oligosaccharides of varying size derived from it were prepared and tested for their ability to fix guinea pig hemolytic complement. antibody and either tetra-, hexa-, or octasaccharide formed only monomeric antibody-hapten complexes and did not show any complement binding. a dodecasaccharide and a 16-sugar residues oligomer formed dimer and trimer immune complexes. these complexe ... | 1976 | 5560 |
| pharmacological characterization of adrenergic receptors of a rabbit cerebral artery in vitro. | in the light of controversy over the functional significance of the abundant sympathetic innervation of large cerebral blood vessels, an in vitro analysis of the adrenergic receptors mediating contractile effects in the rabbit basilar artery was undertaken. beta adrenergic stimulation had no effect, and agents that block norepinephrine (ne) uptake did not alter contractile responses to l-ne. the l-ne dose-response curve could be resolved into two components s-shaped curves: the first had an ed50 ... | 1976 | 5597 |
| effects of droperiodol on cardiovascular system--experimental study. 1. alpha adrenergic blocking action of droperidol on the isolated aortic strip of the rabbit. | | 1976 | 5618 |
| effects of changing levels of glucocorticosteroids on heat exposure in rabbit. | rabbits exposed for one hour to a temperature of 40degrees cand 35-37% humidity showed elevated plasma osmolality and ph. most of the animals were not able to withstand the heat. dexamethasone-treated rabbits under the same conditions withstood the heat better and their plasma ph and osmolality remained constant. metopirone-treated rabbits withstood the heat and showed a rise in plasma osmolality and a slight change in plasma ph. the highest rise in rectal temperature was observed in the metopir ... | 1975 | 5621 |
| glucocorticoid potency and parachor. | | 1976 | 5630 |
| characterisation of eukaryotic ribosomal proteins. | a simple method of two-dimensional polyacrylamide gel electrophoresis is described which affords: (1) high resolution of eukaryotic ribosomal proteins; (2) good recovery of protein in the transfer from first to second dimension; and (3) characterisation of the separated proteins in terms of molecular weights and other electrophoretic properties. using this method, we have characterised 70 proteins in rabbit reticulocyte ribosomes, 30 from the small subunit and 40 from the large subunit. the mole ... | 1976 | 5665 |
| beta adrenergic blockade and the secretory activity of gastric mucosa. | | 1976 | 5714 |
| some biological activities of rabbit anti-interferon serum. | after prolonged immunization of rabbits with a semipurified mouse interferon preparation in freund's incomplete or al-span-oil adjuvant, a specific interferon-neutralizing immunoglobulin was obtained from antiserum with a capacity of neutralizing about 49000 mouse interferon units per ml. the specific activity of the antiserum and immunoglobulin was confirmed in tests in which the interaction of antibodies with the cell surface was ruled out. the antiserum (and the immunoglobulin) neutralized bo ... | 1976 | 5864 |
| characterization of an endotoxic lipopolysaccharide from coxiella burnetii. | phase i coxiella burnetii antigen isolated by phenol extraction from purified suspensions of c. burnetii in phase i is a complex lipopolysaccharide (lps) molecule containing substances typical of the bacterial lps. some endotoxic properties of this c. burnetii lps, namely pyrogenicity and skin epinephrine reaction in rabbits, hypothermia in white rats, lethal effect on chicken embryos or on actinomycin-d-treated mice are similar to those of lps isolated from other gram-negative bacteria. | 1976 | 5871 |
| electron microscopy of virus particles in ultrathin frozen sections. | a procedure with the use of polyethylene glycol (peg) 600 to support biological materials for ultracryotomy was developed. developing and mature virions were demonstrated by electron microscopy in negatively stained frozen sections of vaccinia virus-infected human diploid cells and of herpes simplex virus (hsv) type 1 infected primary rabbit kidney cells. approximate localisation of the virus particles in the cells was possible. | 1976 | 5872 |
| hypothermia and rewarming by peritoneal dialysis and temperature-controlled inhalate. | in 12 rabbits hypothermia and rewarming were induced with temperature-controlled circulating peritoneal dialysis in combination with temperature-controlled hypoxic and hypercapnic gas mixtures. the average cooling time necessary for the esophageal temperature to decrease from 37.7 degrees +/- 0.7 to 20.6 degrees +/- 1.0 degrees c was 81 +/- 34 minutes with a range of 41 to 150 minutes. the average warming time for esophageal temperature to increase from 20.6 degrees +/- 1.0 degrees c to 35.2 deg ... | 1976 | 5972 |
| suppression of renin release by timolol. | the beta-adrenergic blocking agent, timolol, administered to resting rabbits as an i.v. bolus (0.125 mg/kg) sustained by a 2-hr infusion at 0.0625 mg/kg/hr, caused significant depression of plasma renin activity (pra) to 49% of the control level. significant correlations emerged between the fall in mean blood pressure and changes in both heart rate and pra. timolol also antagonized isoprenaline-induced renin release. in anaesthetized normal rats, timolol (0.2 mg/kg i.p.) suppressed mean plasma r ... | 1976 | 5974 |
| drugs and pgo waves in the lateral geniculate body of the curarized cat. v. miscellaneous compounds. synopsis of the role of central neurotransmitters on pgo wave activity. | in the last part of this series we have studied the effects of various drugs on ponto-geniculo-occipital (pgo) waves induced by the benzoquinolizine derivative, ro 4-1284 (pgo(1284)), and by the inhibitor of trypotophan hydroxylase, p-chlorophenylalanine (pgo(pcpa)), and continuously recorder and counted in the lateral geniculate bodies (lgb) of unanaesthetized and immobilizedcats. the major aim of this study was to test the specificity of drug-induced alterations of the pgo wave activity sugges ... | 1976 | 5977 |
| evidence against the participation of the gamma-glutamyltransferase-gamma-glutamylcylclotransferase pathway in amino acid transport by rabbit erythrocytes. | the gsh concentration of rabbit erythrocytes was monitored under conditions of large net transport of alanine, phenylalane and lysine in the absence of glucose. in no case was there an appreciable alteration in gsh concentration during amino acid uptake. it is suggested that the gamma-glutamyltransferase-gamma-glutamylcyclotransferase pathway does not participate in amino acid transport by these cells. | 1975 | 6000 |
| kinetic studies on glucoamylase of rabbit small intestine. | the kinetic properties of a maltase-glucoamylase complex with a neutral ph optimum, purified to homogeneity from the brush borders of the rabbit small intestine, are described. it has a broad range of substrate specificity, hydrolysing di- and poly-saccharides with alpha-1,4 and alpha-1,6 linkages. the km and vmax, values of the enzyme for the various substrates were determined. starch and maltose were its best substrates. the kinetics of hydrolysis of two synthetic linear maltosaccharides, name ... | 1976 | 6006 |
| purification and some properties of acetyl-coenzyme a carboxylase from rabbit mammary gland. | 1. acetyl-coa carboxylase from lactating-rabbit mammary gland was purified to homogeneity by the criterion of polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. 2. use of phosphate buffer throughout the purification gave low recovery of enzyme. consequently, tris buffers were used in the extraction and in selected stages of the purification procedure. 3. the purified enzyme had a specific activity of 5.15 +/- 0.3 mumol of bicarbonate incorporated/min per mg of protein ... | 1976 | 6012 |
| tyrosine aminotransferase activity in the rabbit placenta. evidence for decreased inducibility by insulin and cortisol as a function of gestational age. | tyrosine aminotransferase is present at low activity in the rabbit placenta. the activity can be enhanced by injecting the pregnant mother with insulin or cortisol. this response decreases over the last half of gestation (14-28 days gestational age). | 1976 | 6019 |
| fructose-1-phosphate-6-sulfate as an alternative substrate for aldolase and fructose-1,6-diphosphatase. | | 1976 | 6025 |
| functional heterogeneity and ph-dependent dissociation properties of human transferrin. | human diferric transferrin was partially labeled with 59fe at low or neutral ph (chemically labeled) and by replacement of diferric iron previously donated to rabbit reticulocytes (biologically labeled). reticulocyte 59fe uptake experiments with chemically labeled preparations indicated that iron bound at near neutral ph was more readily incorporated by reticulocytes than iron bound at low ph. the ph-dependent iron dissociation studies of biologically labeled transferrin solutions indicated that ... | 1976 | 6057 |
| biochemical studies of proacrosin and acrosin from hamster cauda epididymal spermatozoa. | | 1976 | 6084 |
| effect of noradrenaline on the electrical and contractile properties of smooth muscle cells in the pulmonary artery. | experiments were performed on the smooth muscle cells of rabbit a. pulmonalis using the microelectrode technique. no spontaneous electrical or mechanical activity was recorded in normal krebs solution. the current-voltage relation in these smooth muscle cells showed marked rectification. no changes in the isometric tension were observed due to the anodal or cathodal stimulating currents. strong depolarization of the muscle cells produced only local potentials on the cathelectrotone which never d ... | 1975 | 6091 |
| activity of nad- and nadp-dependent malate dehydrogenase isoenzymes in the myocardium of rabbits with alloxan diabetes. | isoenzymes nad-and nadp mdh were detected in the cardiac muscle of rabbits by disc electrophoresis in polyacrylamide gel. alloxan diabetes proved to be accompanied by a significant reduction in the activity of mitochondrial nadp mdh (in the reaction of malic decarboxylation) and its increase in cytozol. the activity of nad-mdh (in the reaction of oxyacetate reduction) was also decreased in various isoenzymes in the myocardium (particularly in the mitochondria) in diabetes. insulin restored the c ... | 1975 | 6094 |
| cyproheptadine as an inhibitor of the effects of bradykinin. | a study was made of the influence of ciproheptadin, serotonin and histamine inhibitor, on the effects of exo- and endogenous bradykinin. ciproheptadin inhibited the effect of bradykinin on a section of a guinea pig intestine in vitro, eliminated the hypotensive effect of bradykinin in rats in vivo, and also blocked the constriction of the vessels of the ear in rabbit and a fall of the blood kininogen level caused by the administration of pyrogenal. | 1975 | 6095 |
| the effect of ph on rabbit atrial response to histamine. | the chorontropic response of isolated rabbit atria in normal tyrode's medium increases monotonically with increasing doses of histamine (9 x 10-7 -9 x 10-4 m). plots of the inverse of response against the inverse of concentration were linear; and from these plots were derived values fro the theoretical maximum response at 'infinite' dose and for ph histamine concentration required to evoke a half maximum response. alteration of ph by changing (hco3-) at a constant pco2, (na) and osolality did no ... | 1976 | 6137 |
| biochemical properties of some microsomal xenobiotic-metabolizing enzymes in rabbit small intestine. | comparison of xenobiotic-metabolizing enzymes in rabbit small intestinal and hepatic microsomal fractions showed mainly quantitative differences; most of the activities were two to seven times higher in liver than in intestine. however, udp-glucuronyltransferase activity was higher in intestine than in liver. the apparent absence of benzene hydroxylase in small intestine was the only qualitative difference noticed. aniline hydroxylase, aminopyrine n-demethylase, and aryl hydrocarbon dydroxylase ... | 1976 | 6224 |
| oxisuran reduction by rabbit tissue preparations. | oxisuran, 2-((methylsulfinyl)acetyl)pyridine is reduced to alpha-((methylsulfinyl)methyl-2-pyridinemethanol, oxisuranol, by cytoplasmic enzymes from rabbit liver, kidney, brain, intestine, and lung. the cytoplasmic enzyme from liver is dependent on nadph as cofactor and has an optimal ph of 6.0. enzymatic activity is also present in liver mitochondria but at a lower specific activity. the cytoplasmic extracts catalyze the formation of two oxisuranol products, presumably the diastereoisomers desc ... | 1976 | 6228 |
| effects of beta-adrenoreceptor blocking drugs on adrenergic transmission. | the peripheral actions of beta-adrenoreceptor antagonists on adrenergic transmitter mechanisms have been reviewed. in addition to receptor blockade, beta-adrenoreceptor antagonists may in high concentrations inhibit neuronal uptake of noradrenaline; inhibit monoamine oxidase; inhibit the uptake of noradrenaline into transmitter storage vesicles and inhibit the extraneuronal uptake of noradrenaline. high concentrations of beta-adrenoreceptor antagonists (threshold about 30 mum) also release norad ... | 1976 | 6246 |
| the role of renin in the antihypertensive action of beta-adrenoreceptor blocking agents. | since the original reports suggesting that the antihypertensive action of beta-adrenoreceptor blocking drugs is related to their inhibitory action on renin release, much evidence has been put forward both to refute and support this hypothesis. our studies of the acute and chronic effects of treatment with propranolol in hypertensive patients showed that the antihypertensive action of the drug was of later onset than the initial cardio-depressant and renin-suppressive effects and had little relat ... | 1976 | 6247 |
| purification and properties of d-glyceraldehyde-3-phosphate dehydrogenase from an extreme thermophile, thermus thermophilus strain hb 8. | 1. d-glyceraldehyde-3-phosphate dehydrogenase from an extreme thermophile, t. thermophilus strain hb8, was purified and crystallized. 2. the enzyme was found to possess remarkable heat stability, being slowly inactivated at 90 degrees c. 3. basic kinetic constants and ph profile are reported. the enzyme was activated 25-fold by 90 mm nh4cl, and also by ethanol up to 5-fold at 30 degrees c. 4. the enzyme was found to be far more resistant to urea or sodium dodecylsulfate than the rabbit enzyme. 5 ... | 1976 | 6270 |
| properties of prostaglandin synthetase of rabbit kidney medulla. | the formation in vitro of prostaglandins e2, d2, and f2alpha from arachidonic acid by rabbit kidney medulla homogenate or microsomal fraction is markedly affected by the composition of the incubation medium employed. optimal biosynthesis is obtained in 0.1 m potassium phosphate buffer, with the optimum ph being 8.0--8.8. under these conditions prostaglandin formation is linear up to arachidonic acid concentration of 30 mum. the initial rate of formation of prostaglandin e2 + prostaglandin d2 is ... | 1976 | 6277 |
| alpha-adrenoceptors in the ventricular myocardium: clonidine, naphazoline and methoxamine as partial alpha-agonists exerting a competitive dualism in action to phenylephrine. | tha alpha-sympathomimetic agonists, clonidine, naphazoline, methoxamine, oxymetazoline and phenylephrine were used to further characterize the alpha-adrenoceptors mediating the positive inotropic effect in the isolated papillary muscle of the rabbit heart. the maximal inotropic effects of these amines were compared with the effect of isoprenaline and it was examined whether or not these amines compete for alpha-adrenoceptors. on the papillary muscle stimulated at 0.5 hz, phenylephrine showed a h ... | 1976 | 6287 |
| effect of 1,4-benzodiazepine derivatives on the toxic action of oxygen under increased pressure. | the 1,4-benzodiazepine derivatives (diazepam, nitrazepm, lorazepam, clonazepam and two newly synthetized compounds of this series) increase the resistance of albino rats and rabbits to the toxic effect of oxygen under high pressure (7 and 5.5 atm respectively). the compounds under study are apt to avert over a long period the development of metabolic acidosis which appears following the action of high-pressure oxygen on the body. | 1975 | 6302 |
| comparative characteristics of fluorasizine metabolism in different species of animals and man. | the process of phthoracizine (10-(beta-diethylaminopropiniol)-2-triphthormethylphenothiazine hydrochloride) transformation proceeding in various animal species and in man is similar in grosso modo to biotransformation of chlorpromazine and of compounds related to the latter, including sulphoxidation, dealkylation in the side chain nitrogen, hydroxylation, glucoronoconjugation and the formation of products with the side chain degradation. at the same time, one can note a difference which finds it ... | 1975 | 6307 |
| effect of adrenergic compounds on the brain potentials evoked by stimulation of the dental pulp. | intraventriculare injections have brough evidence that alpha-adrenomimetic norepinephrine and beta-adrenomimetic isopropylnorepinephrine augment in the senso-motor region of the cortex the amplitude of primary responses evoked through a dental pulp stimulation. the alpha-adrenoblocking agents--dihydroergotoxin and phentolamine and the beta-edrenoblocking agent propranolol force down the amplitude of primary responses. the sphere of the drugs action is presumed to be the thalamic nuclei. | 1975 | 6308 |
| effect of izadrin, inderal and netalid on the periodic activity of the rabbit myometrium. | the contractility of an isolated rabbit uterus was studied. it was found that when used in low concentrations (1.10(-9)-1.10(-7) g/ml) isoprenaline, while stimulating the beta-adrenoreceptors of the uterus, inhibits its contractions, whereas in high concentrations (1.10(6)-1.10(-5) g/ml) it is capable of intensifying uterine contractions owing to stimulation of the uterine alpha-adrenoreceptors. dihydroergotoxin (1.10(-6) g/ml), a blocking agent of alpha-adrenoreceptors, averts and eliminates th ... | 1976 | 6311 |
| a lipoxygenase in rabbit reticulocytes which attacks phospholipids and intact mitochondria. | | 1975 | 6318 |
| ovulatory failure: clinical aspects. | | 1976 | 6339 |
| mechanisms of pial artery responses in hypo- and hypertension. | simultaneous study of the pial arterial responses and quantitative changes of the systemic arterial pressure, as well as of the po2, ph and pco2 on the brain surface, showed no correlation between the values and time of changes of the above parameters in rabbits. the vascular responses are concluded not to be caused by the direct action of either the changes of the intravascular pressure, or of the mentioned metabolites on the arterial walls, but rather to be due to another, probably nervous, fe ... | 1976 | 6343 |
| the clinical significance of the concentration of 2,3-diphosphoglycerate (dpg) in erythrocytes (2). | | 1976 | 6400 |
| pathways of the eye's response to topical nitrogen mustard. | we studied the effect of prior corneal herpes simplex infection with its resultant corneal hypesthesia on the irritative response of the rabbit eye to topical nitrogen mustard. both the miosis and the breakdown of the blood-aqueous barrier that follow the application of topical nitrogen mustard were diminished in eyes infected three weeks previously with herpes simplex virus. nonspecific corneal scarring did not affect the response. this suggests again that an axon reflex requiring intact sensor ... | 1976 | 6401 |
| the adrenergic receptors of the intraocular muscles of the human eye. | the adrenergic receptors in man were analyzed using isolated sphincter, dilator, and ciliary muscle strips, dissected from eyebank eyes. the dilator is mainly alpha, the sphincter both alpha and beta, and the ciliary muscle predominantly beta adrenergic. | 1976 | 6403 |
| antibody-dependent cell-mediated cytotoxicity in selected autoimmune diseases. | antibody-dependent cell-mediated cytotoxicity mediated by peripheral blood lymphocytes was studied in patients with systemic lupus erythematosus, polyarteritis nodosa. sjogren's syndrome, and rheumatoid arthritis. the target cells were chicken erythrocytes coated with rabbit anti-chicken erythrocyte antibody. antibody-dependent cell-mediated cytotoxic activity was normal in sjogren's syndrome and rheumatoid arthritis but significantly decreased (p is less than 0.001) in active systemic lupus ery ... | 1976 | 6490 |
| fine structure of three different anti-fluorescein combining sites: induced circular dichroism of hapten bound to autologous and heterologous recombinants. | we have previously reported the sequential appearance in hyperimmunized rabbits of three distinct types of antifluorescein-combining sites that can be distinguished by the characteristic induced circular dichroism (cd) of the bound hapten, fluorescein. such induced cd depends on the configuration of the surrounding residues, and, in the case of anti-fluorescein, can be localized to the configuration of the sub-site which binds the hydroxyxanthenone moiety of fluorescein. in the present investiga ... | 1976 | 6594 |
| the absorption and elimination of metoclopramide in three animal species. | the absorption and elimination of metoclopramide have been studied in the rat, rabbit and dog. thin-layer chromatography followed by photodensitometry was used for the analysis of the unchanged drug and its metabolites. n-de-ethylation is an important phase i metabolic reaction and conjugation with glucoronic acid and sulphate is a major route of metabolism, particularly in the rabbit. the pharmacokinetic parameters after intravenous administration showed little interspecies variation. first ord ... | 1976 | 6647 |
| release of prostaglandin e-like material from perfused mesenteric blood vessels of rabbits. | infusions of noradrenaline (1-3 mug ml(-1) min(-1)) into the mesenteric vascular preparation of the rabbit caused a 2 to 5 fold rise in perfusion pressure and a release of prostaglandin e-like material (3.23 +/- 0.65 (s.e.) ng pge2 equivalents ml(-1)). indomethacin (3 mug ml(-1)) prevented whereas arachidonic acid (0.2 mug ml(-1)) augmented, the noradrenaline-evoked release of a prostaglandin e-like material. the walls of arterioles or precapillary vessels are the proposed site of prostaglandin ... | 1976 | 6648 |
| studies with the international pyrogen standard on the sensitivity and reproducibility of pharmacopoeial pyrogen testing. | rabbits, 27 or 36 in each experiment, were injected with the international pyrogen standard (i.p.st.) in different seasons. the maximum temperature rises were registered, randomized and interpreted according to the requirements of the b.p. (1973), u.s.p. (1970), p. hung. (1970) and p. nord. (1962). although the dose of 3.5 ng kg(-1) i.p.st. proved to be non-pyrogenic as tested in summer, when tested in winter the same dose was qualified pyrogenic (to be rejected) by up to one third of the combin ... | 1976 | 6669 |
| some pharmacological properties of the venom, venom fractions and pure toxin of the yellow-bellied sea snake pelamis platurus. | the effects of the crude venom, four partially purified venom fractions and pure toxin (pelamis toxin alpha) from yellow-bellied sea snake, pelamis platurus, on respiration, blood pressure, heart and skeletal muscle of rabbits have been examined. results indicated that crude venom, a partially purified toxic fraction and pelamis toxin alpha caused initial respiratory stimulant effects followed by respiratory paralysis. in most cases, respiratory paralysis occurred before a profound fall in arter ... | 1976 | 6673 |
| dimethyl sulphoxide, a pyrogen potentiating agent. | | 1976 | 6675 |
| quipazine: a serotoninergic hyperthermic agent in the rabbit. | | 1976 | 6684 |
| a sensitive biological assay for prostaglandin e and acetylcholine. | superfused hamster stomach strip has been used in the bioassay of prostaglandin e2 (pge2) and acetylcholine. the preparation proved very stable and had no spontaneous movements. the sensitivity to pge2 (and pge1) was in the range of 10(-9) g ml(-1) and to acetylcholine 10(-12) g ml(-1). after treatment with physostigmine the sensitivity to acetylcholine was 10(-15) g ml(-1). in acetylcholine determination the preparation is more sensitive than any other method. | 1976 | 6690 |
| mass spectrometric determination of prostaglandin e2, f2alpha and a2 in the cortex and medulla of the rabbit kidney. | | 1976 | 6731 |
| the discovery of uptake of adrenaline in 1930-1933 and the development of the adrenergic fibre from a cholinergic fibre. | | 1976 | 6737 |
| sympathomimetic bronchodilators and animal models for assessing their potential value in asthma. | | 1976 | 6741 |
| modern views on the pathogenesis of fever and the mode of action of antipyretic drugs. | | 1976 | 6743 |
| the break-up time of artificial pre-ocular films on the rabbit cornea. | | 1976 | 6758 |
| further studies on angiotensin tachyphylaxis. | rabbit aorta and rat fundus do not display tachyphylaxis to angiotensin ii (asp1)angiotensin ii) at 37 degrees c. these tissues do, however, display tachyphylaxis to sar1angiotensin ii (n-methylglycine 1 angiotensin ii) and dimethylglycine angiotensin ii, respectively, at this temperature. at 22 degrees c, both tissues displayed rapid tachyphylaxis to asp1angiotensin ii which was reversed on prolonged incubation in peptide-free medium. at 37 degrees c, but at a lower than normal ph (ph 6.5 inste ... | 1976 | 6785 |
| studies on adrenal phenylethanolamine n- methyltransferase (pnmt) with s k & f 64139, a selective inhibitor. | sk&f 64139 is a potent, reversible inhibitor of phenylethanolamine n-methyltransferase; its ic50 concentration in our standard assay system was 1 x 10(-7) m. kinetically, the compound is a competitive inhibitor with respect to norepinephrine but is uncompetitive when s-adenosylmethionine is the variable substrate. in contrast to a previously reported compound (sk&f 7698), the drug is only a weak alpha receptor antagonist (kb = 6 x 10(-6) m). in both the rat and squirrel monkey, sk&f 64139 produc ... | 1976 | 6786 |
| the potentialities of some antihistaminics in preanaesthetic medication. | a detailed study has been carried out on the potentialities of antazoline and chlorpheneramine (c.p.m.) in preanaesthetic medication. it included a determination of their sedative action, effects on cardiovascular system and any possible anticholinergic and antiarrhythmic properties. both drugs depressed spontaneous activity in mice. antazoline produced hypotension in chloralosed dogs and spinal cats (a peripheral action). both produced a negative inotropic action on isolated rabbit's heart. on ... | 1975 | 6857 |
| the influence of neuromediators injected into nucleus caudatus on bioelectrical seizure activity of amygdala. | the influence of electrical and chemical stimulation of nucleus caudatus (nc) on bioelectrical seizure activity of amygdala (am) was studied in rabbits. the electrical stimulation of nc inhibits seizures in am induced by the administration of picrotoxin into this nucleus. dopamine (da) and cholinomimetics-metacholine and neostygmine-applied into nc inhibit seizures in am. noradrenaline (na) acts biphasically, first potentiating and then inhibiting seizures in am. serotonin (5-ht) and glutamic ac ... | 1976 | 6950 |
| observations on ouabain binding and membrane phosphorylation by the sodium pump. | | 1976 | 6964 |
| mechanisms and regulation of biosynthesis of saturated fatty acids. | | 1976 | 6981 |
| is there a sleep transmitter? | | 1976 | 6995 |
| the effects of an alpha blocking agent on the respiratory distress syndrome with disseminated intravascular coagulation in the newborn infants and experimental animals. | | 1976 | 7028 |
| effect of atropine sulfate, papaverin hc1 and antihistaminic preparations on isolated organs of various experimental animals. | | 1975 | 7039 |
| species and phenobarbitone-induced differences in the kinetic constants of liver microsomal harmine o-demethylation. | 1. the apparent kinetic constants for the o-demethylation of harmine to harmol by 10 000 g supernatant fractions from livers of mice, rats, guinea-pigs, rabbits, cats and cows have been determined. the km values were 10-39 mum and vmax 0-25 and 1-65 nmol/mg protein/min. 2. optimal conditions of incubation time and nadp requirements differed between species. in all species except cat and cow the rate of o-demethylation of harmine was linear for 5 min, but in the latter species was linear for 15 m ... | 1976 | 7043 |
| thrombocyte function in bromcarbamide poisoning. | | 1975 | 7051 |
| treatment of intestinal paresis by intravenous administration of gutimine. | | 1976 | 7067 |
| blood regeneration in sensitized animals after blood loss on the background of aseptic inflammation. | | 1976 | 7070 |
| combination of r3365 (piritramide) and dehydrobenzperidol in animals. respiratory effects and analgesia. | 1 -- respiratory depression has been studied in the no anaesthetized rabbit by analysis of the blood gases after an injection of piritramide (r 3365) and dehydrobenzperidol. 2 -- the respiratory depression induced by piritramide alone is less important after an injection of dehydrobenzperidol; the most interessant cases are: -- mixture dehydrobenzperidol 400 mug/kg. pirittramide 1000 mug/kg; --dehydrobenzperidol (400 mug/kg) injected 15 minutes before piritramide (1000 mug/kg). 3 -- the addition ... | 1975 | 7163 |
| central noradrenergic control of blood pressure. | | 1976 | 7183 |
| toxicology of inhalation anesthetics and metabolites. | | 1976 | 7186 |
| cyclic nucleotide phosphodiesterases of rabbit renal cortex. characterization of brush border membrane activities. | | 1976 | 7201 |
| biosynthesis of prostaglandins in rabbit kidney medulla. properties of prostaglandin synthase. | a simple radioactive-substrate assay for prostaglandin synthase (ec 1.14.99.1), which uses t.l.c. to measure simultaneously different prostaglandins synthesized from one precursor substrate, was developed. rabbit kidney-medulla prostaglandin synthase catalyses the formation of prostaglandin e2, prostaglandin f2alpha and prostaglandin d2 from arachidonic acid. fractionation of crude homogenates indicated that the microsomal fraction possessed the highest specific activity of prostaglandin synthas ... | 1976 | 7235 |