| an immunoperoxidase technic for the demonstration of the hepatitis b surface antigen in human livers. | a sensitive method for demonstrating the site of hepatitis b surface antigen (hbsag) in fixed tissues embedded in either paraffin or araldite is described. the method employs the peroxidase-rabbit antiperoxidase linkage through goat antirabbit to rabbit anti-hbsag. in staining hepatitis antigen in agar, comparison of fixation (using three common fixatives) with unfixed precipitation arcs revealed no recognizable differences in antigenicity induced by fixation. the method allows confirmation of p ... | 1976 | 57716 |
| specificity of antisera produced against bleomycin. | the specificity of antisera produced in a goat or rabbit for use in bleomycin radioimmunoassay has been examined using competitive experiments employing several bleomycin analogs. these studies demonstrate that the antiserum distinguished alterations in both the nucleus and the terminal structure of the molecule. removal of the terminal structure or addition of an aminosugar moiety in the bleomycin nucleus resulted in a significant decrease in immunoreactivity. antisera produced in goat and rabb ... | 1977 | 72602 |
| studies in immunodermatology. vi. if studies of autoantibodies to the stratum corneum and of in vivo fixed igg in stratum corneum of psoriatic lesions. | indirect immunofluorescent (if) tests on sections of normal human skin reveal the presence of antibodies to the stratum corneum in most normal human sera. sera absorbed with hyperkeratotic scales gave negative reactions. these reactions appeared to correspond to those of the stratum corneum antibodies which were first detected by krough and tonder by immune adherence, though this remains to be documented since the conjugate alone stained the stratum granulosum in a pattern comparable to that of ... | 1975 | 46852 |
| outer-membrane protein and lipopolysaccharide serotyping of neisseria meningitidis by inhibition of a solid-phase radioimmunoassay. | a new procedure involving inhibition of a solid-phase radioimmunoassay was developed for specific determination of the outer-membrane protein and the lipopolysaccharide (lps) serotypes of meningococci. antigen was allowed to bind to the wells of a polyvinyl microtiter plate and then reacted with a limiting amount of homologous antibody which had been preincubated with buffer or a standard concentration of inhibiting antigen. the amount of antibody bound per well was quantitated by incubation wit ... | 1977 | 72735 |
| multihormonal induction of hepatic alpha2u-globulin mrna as measured by hybridization to complementary dna. | a procedure is presented for the preparation of a (3)h-labeled complementary dna (cdna) specific for the mrna coding for alpha(2u)-globulin, a male rat liver protein under multihormonal control that represents approximately 1% of hepatic protein synthesis. rat liver polysomes are incubated with monospecific rabbit antiserum to alpha(2u)-globulin, which binds to the nascent alpha(2u)-globulin chains on the polysomes. these antibody-polysome complexes are then adsorbed to goat antiserum to rabbit ... | 1977 | 73184 |
| serum-derived immunosuppressive substances. iii. regulation of the immune response by human serum alpha-globulin fractions: the dose-response relationship. | suppression of the plaque-forming cell response in mice following injection of substances fractionated from pooled normal serum alpha-globulin was investigated. the dose-response relationship for fractions obtained by ion-exchange chromatography show that a single preparation has both enhancing and suppressive activities which are revealed at different doses. whether this observation reflects the sum of activities of a number of molecular species remains to be determined. the immune responses to ... | 1977 | 73512 |
| species specificity in thermostable and ethanol insoluble tissue antigens. ii. further immunization of rabbits with bovine kidney preparation. | | 1977 | 74202 |
| species specificity in thermostable and ethanol insoluble tissue antigens. iii. further immunization of ruminants with antigens from other ruminants and whale (balaenoptera acutorostrata). | | 1977 | 74203 |
| differential antigenicity of human salivary histidine-rich polypeptides in goats and rabbits. | | 1977 | 74246 |
| induction of a t-cell specific antigen on bone marrow lymphocytes with thymus rna. | expression of a rabbit t-cell specific antigen can be induced on bone marrow lymphocytes following exposure to an rna extract obtained from the thymuses of young rabbits. the presence of the antigen was demonstrated using goat anti-rabbit t-cell serum in a complement-dependent cytotoxicity assay. the t-cell antigen first appeared 3 h after addition of the thymus rna to bone marrow cell cultures and the maximum number of cells expressing the t-cell antigen was observed within 24 h. rna obtained f ... | 1978 | 75176 |
| solid-phase radioimmunoassay method for determination of escherichia coli enterotoxin. | the development of a solid-phase radioimmunoassay procedure for the determination of escherichia coli enterotoxin(s) is described. radioiodinated e. coli enterotoxin with about three radioiodine atoms per toxin molecule is, by the criterion of electrophoresis, identical to the unlabeled toxin. goat anti-e. coli-enterotoxin antibody was coupled to polystyrene tubes and served as a solified toxin binder in the reported procedure. various conditions necessary for the optimization and standardizatio ... | 1978 | 75844 |
| immunohistochemical study of a large molecular fragment of thyroglobulin in parafollicular cells. | thyroglobulin-like immunoreactivity of the parafollicular cells was studied by an immunoperoxidase bridge technique using antisera against dog thyroglobulin fragments. 1. the dog parafollicular cells were specifically stained by anti-peak i (27s and larger components fraction) antiserum absorbed with peak ii (19s fraction). by this method, they were easily distinguishable from the non-reactive follicular cells and colloid droplets. more sensitive staining of the parafollicular cells was possible ... | 1978 | 76516 |
| human alpha-fetoprotein in the epidermal cells of bowen's disease. | human alpha-fetoprotein (hafp) was demonstrated in clusters of atypical cells of an extensive bowen's disease involving the pubic area. frozen sections were studied by indirect immunofluorescence using monospecific rabbit anti-hafp antiserum or purified rabbit anti-hafp antibody followed by staining with fluorescein isothiocyanate (fitc)-labeled goat anti-rabbit gamma-globulin and by a direct technique using fitc-labeled rabbit anti-hafp fab2 fragments. sections of fetal liver served as positive ... | 1978 | 76665 |
| [the animal as a symbol]. | | 1978 | 77068 |
| complement-mediated release of histamine from human leukocytes. | activation of either the alternative or classical pathway of complement generated a factor which induced release of histamine from both non-allergic and allergic human basophils. this factor probably is derived from the complement system since 1) its formation was associated with loss of c3 activity in human serum, 2) chemotactic factor, probably also a complement product, was generated simultaneously, 3) heat inactivation blocked its formation, 4) anti-c3 and anti-c5 blocked formation of the fa ... | 1975 | 46261 |
| rabies vaccination. | | 1978 | 77143 |
| quantitative measurement of mouse igg subclasses with the use of heteroantisera: the importance of allotype considerations. | double antibody radioimmunoassays have been used to determine the quantities of igg1, igg2a and igg2b in samples of normal serum igg from balb/cj, akr/j and c57bl/6j inbred mice. the assays employed subclass-specific goat antisera which had been prepared with balb/c myeloma proteins as immunogens and as immunoabsorbents. 125i-labeled balb/c myeloma proteins were used as probes. results indicate that partial resolution of mouse igg subclasses was achieved by ion exchange chromatography on deae-se ... | 1977 | 66274 |
| a rapid radioimmunoassay for human alpha-fetoprotein. | our previous double antibody radioimmunoassay for human afp was run at 4 degrees c and required 48 h for completion. a method is described which permits the assay to be performed within 5 h due to an increased incubation temperature and the use of polyethylene glycol to precipitate the antigen-antibody complex. the modified radioimmunoassay was standardized against an international reference preparation of human afp. characteristics of this assay are discussed. | 1978 | 77746 |
| immunochemical analyses of a major antigen of mycobacterium szulgai. | unheated culture filtrate of mycobacterium szulgai and a homologous goat antiserum were prepared. immunoelectrophoretic analysis demonstrated a dominant anodal antigen in the culture filtrate. by the use of diethylaminoethyl-cellulose chromatography, a fraction designated msp, which was rich in this anodal antigen, was recovered. the major antigen of msp was demonstrated to have partial identity with reference mycobacterial antigen 6, and evidence was obtained for separate shared and specific an ... | 1977 | 67158 |
| rift valley fever in egypt, 1978. | | 1978 | 80678 |
| affinity chromatographic purification of the pregnancy zone protein. | an immunospecific affinity chromatographic method for purification of human pregnancy zone protein (pzp) directly from serum is described. highly purified goat-anti-human pzp-immunoglobulin was applied as a ligand. recovery of pzp varied from 56--75%. the impurities constituted maximally 5--10% of the total protein in the eluate. the purification factor was approximately 100. | 1978 | 81241 |
| [use of heterologous antistaphylococcal gamma-globulin in treating suppurative mastitis]. | the effectiveness of heterologic antistaphylococcal gammaglobulin was studied in the clinic in 67 cases of acute lactogenous mastitis. the activity of the preparation was 200 a. e/ml. the bezredko method of injection was used--l ml once a day during 5 days. the treatment resulted in the decrease of intoxication, purification of the surgical wound, normalization on non-specific protection factors, rise of antistaphylococcal antitoxin titers and decrease of autosensitization phenomena. | 1978 | 81560 |
| isolation of specific antibody under conditions of low ionic strength. | specific rabbit and goat antibodies to tobacco mosaic virus, bovine serum albumin and human gamma globulin have been isolated by acid dissociation from the antigen in the absence of salt. maximum recovery of 96-100% of active antibody occurred at ph 2.8-2.9. the amount of antibody recovered within any given ph range was highly reproducible. the average antibody affinity was found to be correlated with the pth of dissociation and to decrease exponentially with the length of exposure to acid condi ... | 1977 | 68980 |
| b-lymphocytes activation by the fc region of igg. | strong stimulation of dna synthesis (up to 150-fold) and blast transformation can be induced in mouse spleen cells by fc fragments of human igg. the mitogenic response is optimal on day 5 of culture and is dependent on the concentration of fc fragments with a sedimentation rate of 3-5s. intact igg is also stimulatory, but only when modified by heat aggregation, and produces only a 10-fold increase in [3h]thymidine uptake. the stimulation by aggregated igg is dependent on the fc portion, since ag ... | 1977 | 68990 |
| idiotypic cross-reactivity between antibodies of different specificities. | cross-idiotypic specificity has been demonstrated between antibody populations of different specificities using antibodies directed toward human sickle cell hemoglobin (hbs). a site-specific antibody directed toward the beta6-position of hbs, anti-val, was used to elicit an anti-idiotypic response in rabbits. using this anti-idiotypic serum, idiotypic cross-reactivity was demonstrated between antibody populations that bind to human adult hemoglobin (hba). it was demonstrated that in the case of ... | 1978 | 82603 |
| eperythrozoon ovis. i. serological diagnosis of infection by the indirect immunoflurescent antibody test. | the indirect immunofluorescent antibody test (ifat) was evaluated for the detection of eperythrozoon ovis infection in sheep. the test first became positive, on the average, 10 days following exposure to e. ovis and remained positive for 24 months, the longest period checked. reciprocal titres in the first three days after becoming positive in the ifat were between 4 and 80, but rose to 640 within 9 weeks; in general titres were between 4 and 640, exceptionally titres of 1280 were obtained. nega ... | 1978 | 83044 |
| evolution of an idiotypic determinant: anti-val. | an antibody population which reacts only with human sickle cell hemoglobin (hbs) and not with normal human hemoglobin, has been isolated from goat, sheep, and guinea pig antisera. these antibody populations termed anti-val (val), isolated from an individual goat (no. 6) and sheep (no. 26) have been used to elicit anti-idiotypic responses in rabbits. these anti-idiotypic sera were used to study the idiotypic cross-reactions between the goat and sheep anti-val. strong cross-reactions were present ... | 1977 | 69001 |
| relationship between leukemia antigens and stimulation in mixed leukocyte culture. | leukemia cells from 13 of 27 patients stimulated lymphocytes of their hla-identical siblings in mixed leukocyte cultures (mlc). stimulation correlated with high background uptake of tritiated thymidine and high percentages of cells that stained with fluorescein-conjugated polyvalent goat antiserum against human immunoglobulin. results were similar to those reported with fractionated autologous lymphocyte subpopulations in normal individuals. investigation of leukemia-specific stimulation in mlc ... | 1977 | 69037 |
| broadly reacting precipitating and agglutinating antigen of leptospirae. | a saprophytic leptospira biflexa strain of equine origin was found which cross-reacts with immune rabbit antisera to 14 pathogenic leptospira interrogans serotypes. sera from goats experimentally inoculated with the saprophyte showed multiple low-level cross-agglutination reactions against a battery of live l. interrogans serotypes. sonically treated and saline-extracted suspensions of the l. biflexa strain and serotypes canicola, icterohaemorrhagiae, and pomona yielded a common precipitating pr ... | 1978 | 83327 |
| binding of protein to polystyrene in solid-phase immunoassays. | | 1977 | 69446 |
| legionnaires' serotypes. | | 1979 | 84117 |
| binding specificity of rabbit anti-guinea pig epidermal cell sera: comparison of their receptors with those of concanavalin a and pemphigus sera. | rabbit anti-guinea pig epidermal cell sera (aes) which were prepared by immunizing rabbits with enzymatically dispersed viable guinea pig epidermal cells were shown to react to the intercellular substances of stratified squamous epithelia of guinea pigs and monkeys in a pattern similar to that seen with concanavalin a (cona) or pemphigus sera (ps) by immunofluorescence. reciprocal blocking tests were performed on guinea pig lip mucosa after removal of the non-specific binding substances between ... | 1979 | 84486 |
| equine markers genes. polymorphism for group-specific component (gc). | polymorphism of equine gc protein was demonstrated by immunofixation electrophoresis with a goat anti-human gc antibody. three different phenotypes, f, fs and s, were found. family data supported the genetic theory of two autosomal codominant alleles, gcf and gcs. both alleles occurred in standardbred, thoroughbred and arabian horses and in shetland ponies. a frequency of 0.23 for gcs in the american standardbred horse indicates the system should be useful for problems of identification and pare ... | 1978 | 84540 |
| [animal experimental studies of hemolysis following extracorporeal blood irradiation]. | in experimental studies on animals, extracorporal blood irradiation was performed in 3 sheeps and 1 goat by means of 500 ci 137 cesium source. the sheep died of the sequelae of narcosis, with anaemia being only moderately marked. in the goat a transit dosis of 466566 rad could be applied before the animal died of the sequelae of haemolytic anaemia. | 1978 | 85590 |
| tissue distribution of human alpha1-microglobulin. | human alpha(1)-microglobulin was isolated from the urine of patients with tubular proteinuria, and its molecular weight was established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 33,000 daltons. the carbohydrate content was 21.7%. anti-alpha(1)-microglobulin serum was prepared and observed to react monospecifically in gel diffusion to purified alpha(1)-microglobulin, as well as to normal human serum and urine. sera from the domestic chicken, mouse, rat, rabbit, dog, calf, co ... | 1979 | 85635 |
| serological definition of the lentivirus group of retroviruses. | the major polypeptides of visna viruses and other lentiviruses have been isolated and shown to be closely related if not identical in radioimmunoassays. by this criterion the lentiviruses form a distinct group of retroviruses unrelated to spuma viruses, mammalian and avian retroviruses that cause tumors, and unclassified retroviruses of cattle and horses. two sera obtained from goats immunized with mason-pfizer monkey virus or squirrel monkey virus reacted with visna p30. additional data suggest ... | 1979 | 85722 |
| effects of anti-guinea-pig beta 2-microgloblin antibodies on lymphocyte transformation induced by specific antigens or mitogens. | the effects of a goat anti-guinea-pig beta 2-microglobulin antiserum (a beta 2m) on lymphocyte transformation, induced by specific antigens or mitogens, were studied. a beta 2m was found to exert inhibitory effects on antigen stimulation, with three different antigens (purified protein devivative, hen egg-white lysozyme, and ovalbumin), and on stimulation induced by the 't-cell mitogens', concanavalin a and phytohaemagglutinin, and the 'mixed mitogen', pokeweed mitogen. stimulation induced by th ... | 1979 | 86202 |
| immunochemical, serologic, and immunologic properties of major antigens isolated from the legionnaires' disease bacterium. observations bearing on the feasibility of a vaccine. | two antigens were isolated from each of three strains of the legionnaires' disease (ld) bacterium. one antigen was serotype-specific; the other cross-reacted with strains of ld bacteria of different serotypes. the serotypic antigens contained all the major branched-chain fatty acids characteristic of ld bacteria and were a lipid-protein-carbohydrate complex. electrophoresis resolved the serotypic antigen of knoxville 1 strain into four protein bands and one glycoprotein band with molecular weigh ... | 1979 | 86314 |
| binding properties of goat igm anti-dinitrophenyl antibodies. | goats immunized over 2 months with low doses of 1 mg/kg of dinitrophenylated salmonella typhimurium responded with low levels of anti-dnp antibodies restricted to the igm class. the purified antibodies show low association constants (ka between 10(4) of 10(5) l/m), a high degree of homogeneity (heterogeneity indices alpha between 0.7 and 0.9) and ten combining sites when tested against dinitrophenyl-lysine as ligand by equilibrium dialysis. these binding properties remained unchanged during the ... | 1978 | 86504 |
| a partial characterization of hepatitis b e antigen. | partially purified hepatitis b e antigen (hbeag) was prepared by ultracentrifugation, ammonium sulfate precipitation, and molecular sieve chromatography of sera obtained from asymptomatic carriers of hepatitis b surface antigen. the antigenic specificity of the hbeag preparations was investigated further with affinity chromatography. the results indicated that hbeag is distinct and separable from dna polymerase activity. columns coupled with either goat igg prepared from antiserum to human igg o ... | 1979 | 86589 |
| specificities of antibodies to acetylcholine receptors. | antisera to acetylcholine receptor purified from the electric organs of torpedo and electrophorus were highly species specific, but showed limited cross-reaction with receptors from all mammalian species tested. antisera to receptor purified from rat muscle cross-reacted extensively with receptor from the muscles of other mammals. antibodies to receptor from electrophorus cross-reacting with receptor from torpedo were affinity purified. subsequently these showed increased cross-reaction with rec ... | 1978 | 86951 |
| antibody-dependent cellular cytotoxicity against murine leukemia viral antigens: studies with human lymphoblastoid cell lines and human peripheral lymphocytes as effector cells comparing rabbit, goat, and mouse antisera. | a thymic lymphoblastoid cell line derived from a new zealand black mouse produces murine leukemia virus (mulv) and was used as a target in model systems for the in vitro study of antibody-dependent cellular cytotoxicity (adcc). several human lymphoblastoid cell lines were investigated as potential effector cells. the most promising (raji cells) bound to antibody-coated target cells but caused only modest levels of adcc at 25:1 effector-to-target cell ratio with substantial lysis in the absence o ... | 1977 | 70469 |
| antibody to influenza virus matrix protein detects a common antigen on the surface of cells infected with type a influenza viruses. | antisera to the type-specific internal influenza virus matrix (m) protein of a type a influenza virus were produced in goats. in the presence of complement, anti-m serum was cytotoxic for target cells which were infected with a variety of serologically distinct type a influenza viruses, but did not react with type b influenza virus-infected cells. absorption experiments indicated that anti-m serum detected a common antigen(s) on the surface of type a-infected cells. this serological cross-reacti ... | 1977 | 70496 |
| synergistic cytotoxicity. i. characterization of a heat labile plasma fraction that induces nonspecific cytotoxicity by human mononuclear cells. | the mechanism by which non-immune mononuclear cells recognize invading foreign material and are activated for cytotoxic attack was studied in a model system employing human mononuclear cells, fresh plasma, and 51cr-labeled xenogeneic target erythrocytes. in these experiments, fresh antibody-depleted plasma or mononuclear leukocytes alone were poorly cytotoxic to xenogenic erythrocytes. however, these target cells were rapidly lysed when both fresh antibody-depleted plasma and mononuclear cells w ... | 1979 | 87417 |
| the fine specificity of regulatory t cells. i. hen egg-white lysozyme-induced suppressor t cells in a genetically nonresponder mouse strain do not recognize a closely related immunogenic lysozyme. | | 1979 | 87419 |
| the activation of guinea pig t lymphocytes by anti-beta 2-microglobulin serum. | in order to study further the role of beta 2-m in the regulation of the immune response, we have examined the effects of a goat anti-guinea pig beta 2-m serum on a number of t lymphocyte functions in vitro. anti-beta 2-m serum produced a marked inhibition of the response of peritoneal exudate t cells to antigen and mitogen stimulation. surprisingly, a marked activation of lymph node t lymphocyte proliferation was observed in the absence of antigen or mitogen stimulation. this stimulatory effect ... | 1979 | 87432 |
| moloney virus-induced cell surface antigens and histocompatibility antigens are located on distinct molecules. | | 1979 | 87439 |
| the activation of the alternative complement pathway by fetal calf serum and mouse thymocytes. | mouse thymocytes activated the alternative complement pathway of mouse serum in the presence of heated fetal calf serum. the activation required c3 from the fetal calf serum but was independent of antibody either in the murine or bovine serum. no other murine cells tested, including erythrocytes, peripheral blood lymphocytes, lymph node cells, spleen cells, and various cultured cell lines, activated the alternative complement pathway as effectively as thymocytes. in addition, sera from species o ... | 1979 | 87451 |
| maturation of the lymphoid system. i. induction of tolerance in neonates with a t-dependent antigen that is an obligate immunogen in adults. | a/j mice displayed a striking ontogenetic difference in the capacity to respond to dnp-ficoll, a t-independent antigen, and to aggregated human gamma-globulin (ahgg), a t-dependent antigen. thus, whereas responses to dnp-ficoll of 4-day-old mice were similar in magnitude to those of adult animals, responses to ahgg did not become pronounced until mice were some 30 to 40 days of age. the inability of young animals to respond to ahgg was reflective of a negative consequence of lymphocyte/antigen i ... | 1979 | 87468 |
| macrophage-lymphocyte interaction in graves' disease and hashimoto's thyroiditis. | the formation of macrophage-lymphocyte rosettes was studied in lymphocyte cultures from patients with graves' disease; hashimoto's thyroiditis, other thyroid diseases and control subjects; the cultures were incubated with normal human thyroid and other non-specific antigens. at the end of incubation, the cell pellets were smeared on slides, stained with wright's stain and the number of rosettes determined under the microscope. the membrane immunofluorescence technique was employed to identify wh ... | 1979 | 88154 |
| tests on three antisera and subsequent development of radioimmunoassay for different regions of human parathyrin. | | 1979 | 88403 |
| a simple spot technique for thin layer immunoassays (tia) on plastic surfaces. | a simple and sensitive technique for visualization of antigen--antibody reactions is described. the property of many antigens to become adsorbed firmly on to a hydrophobic polystyrene surface while retaining their serological reactivity is taken advantage of. on a surface with adsorbed antigen the corresponding immune serum is applied spot-wise. the antigen--antibody reaction areas on the surface are characterized by a distinct hydrophilic condensation pattern when exposed to water vapour. the r ... | 1977 | 71329 |
| suppression and reversal of allergic encephalomyelitis in rhesus monkeys with basic protein and peptides. | we have extended earlier studies on the suppression of clinically evident experimental allergic encephalomyelitis (eae) in monkeys, repeated injections of human basis protein. the results confirm that after suppressive treatment, recovered animals remain clinically normal and do not show spontaneous recurrence of symptoms. however, recovered animals are susceptible to eae upon renewed challenge, and they develop the disease more rapidly and more severely than after the initial challenge; resuppr ... | 1979 | 88679 |
| fetal specific serum proteins of the indian buffalo. | fetal specific serum proteins of the indian buffalo were investigated using rabbit antibuffalo fetal serum, absorbed with lyophilised adult buffalo serum, and subjected to gel diffusion and immunoelectrophoretic analysis. afp was found to be the major fetal specific serum protein of the indian buffalo. a close antigenic relationship between the fetal specific serum proteins of the buffalo, sheep and goat and a distant relationship between these proteins of ruminants and man was found by immunolo ... | 1979 | 88787 |
| the spleen focus-forming virus (sffv)-specific neoantigen shares cross-reactive determinants with mink cell focus-inducing (mcf) virus gp70. | | 1979 | 88803 |
| cea-like antigens in different histologic types of human lung carcinoma. | comparative studies on the presence and immunological reactivity of cea and cea-like antigens in lung and digestive tract cancers were performed. specific goat and rabbit anti-cea immune sera were used. immunofluorescence studies revealed in histologically different types of the lung carcinomas the presence of the cross reacting antigen with classical cea. absorption procedures, and double diffusion tests have shown the existence of two at least antigenic determinants in cea molecule: 1. the det ... | 1979 | 89630 |
| human leukaemia-associated antigens expressed by acute myelocytic leukaemia cells and their detection by heterologous antisera. | antisera against human acute myelocytic leukaemias were tested in complement-dependent in-vitro cytotocity tests against leukaemia cells and normal cells as targets. after absorption with erythrocytes and spleen cells from allogeneous donors the antisera reacted with leukaemia cells, but not with leukocytes from bone marrow and the peripheral blood of children in remission, lymphocytes from healthy donors, enriched b-lymphocytes, enriched t-lymphocytes, pha-induced blasts and cord blood lymphocy ... | 1979 | 89982 |
| human leukaemia-associated antigens expressed by acute lymphocytic leukaemias and their detection with heterologous antisera to t, b-, and non-t-non-b subtype al blasts. | antisera from rabbits and goats against subtypes of acute lymphocytic leukaemia (all with t-cell markers, all with b-cell markers, non-t-non-b all) were tested for their specificity in complement-dependent in-vitro cytotoxicity testing. after absorption of the fivefold diluted antisera with erythrocytes and spleen cells of allogenous donors they reacted with all cells, but not with leukaemias of other types (aml, cll, cml), lymphocytes of healthy donors, enriched b-lymphocytes, enriched t-lympho ... | 1979 | 89983 |
| a radioimmunoassay for virus antibody using binding of 125i-labelled protein a. | an assay for virus antibodies using protein a from staphylococcus aureus is described. type b and type c rna tumour viruses adsorbed on to polystryrene microtitre plate wells were incubated with antiserum and then with 125i-labelled protein a (i-pa) and bound radioactivity was determined. technical details such as labelling, antigen concentration, storage of i-pa are reported. the specificity of the reaction was investigated in detail by competition experiments with purified unbound homologous v ... | 1979 | 90116 |
| analysis of antigens in mycobacterium paratubercuolsis. | | 1979 | 90456 |
| cross-neutralization tests on sheep pox, goat pox and contagious pustular dermatitis viruses. | in cross-neutralization tests in cell cultures, sheep pox and contagious pustular dermatitis (cpd) hyperimmune sera did not cross-react in the heterologous system. but goat pox hyperimmune serum neutralized both sheep pox and cpd viruses revealing a one-way serological relationship of goat pox virus with both sheep pox and cpd viruses. | 1979 | 90458 |
| purification of a new high activity form of glucose-6-phosphate dehydrogenase from rat liver and the effect of enzyme inactivation on its immunochemical reactivity. | a new form of cytoplasmic glucose-6-phosphate dehydrogenase (e.c.1.1.1.49) was purified from rat liver by protamine sulfate precipitation, ammonium sulfate fractionation, ion exchange chromatography with diethylaminoethyl cellulose, and affinity chromatography with cibacron blue agarose and nadp agarose. this form of the enzyme has a specific activity of over 600 units/mg of protein and gives essentially a single band by polyacrylamide gel electrophoresis. the form of the enzyme isolated by this ... | 1979 | 90677 |
| relationships between beta2-microglobulin and alloantigens coded for by the major histocompatibility complexes of the rabbit and the guinea pig. | treatment of rabbit and guinea pig lymphocytes with fab' fragments of anti-beta2-microglobulin completely inhibited the cytotoxic effects of alloantisera to rla or gpla antigens, respectively. aggregation of beta2-microglobulin on the lymphocyte surface by successive incubations with goat anti-beta2-microglobulin on the lymphocyte surface by successive incubations with goat anti-beta2-microglobulin and f(ab')2 fragments of rabbit anti-goat igg also made rabbit lymphocytes resistant to lysis by a ... | 1977 | 72407 |
| [study of the subclasses of immunoglobulin g. ii. qualitative and quantitative characteristics of igg subclasses using antisera systems]. | bispecific antisera, or "antisera-systems", containing class- and subclass-specific antibodies to igg were obtained from rabbits, goats and guinea pigs after brief courses of immunization with purified g1, g2, g3 and g4 paraproteins. after the elimination of antibodies to light chains by adsorption these antisera were tested in immunoelectrophoresis and radial immunodiffusion in gel with sera containing g paraproteins of different subclasses. in immunoelectrophoresis double lines and in radial i ... | 1979 | 91284 |
| acute hemorrhagic pancreatic necrosis in mice: lack of a pathogenetic role for complement. | feeding a choline-deficient diet containing 0.5% dl-ethionine induces an acute hemorrhagic pancreatitis in 100% of young female mice. evidence for deposition of the third component of complement (c3) on acinar cell plasma membranes was sought, during the inductive stages, by a sandwich immunofluorescence technique. such a localization could not be demonstrated even though the method is capable of detecting less than 8 x 10(-5) microgram of protein/mm2 of cell membrane. artifactual binding of imm ... | 1979 | 91291 |
| comparative morphology and mucus histochemistry of the ruminant cervix: differences between crypt and surface epithelium. | | 1979 | 91389 |
| presence of plasma cells binding autologous antibody during an immune response. | spleen and other lymphoid tissues of rabbits immunized with human serum albumin (hsa) and human lactoferrin (lf) were examined for the presence of cells forming anti-idiotype antibodies. to detect these cells, igg, f(ab')2, or fab' of specific antibodies were isolated, fluorochrome-tagged with tetramethylrhodamine isothiocyanate, and used as an idiotypic marker to detect splenic plasma cells that are producing anti-idiotypic antibody. by this procedure, we were able to demonstrate anti-idiotypic ... | 1979 | 91659 |
| xenoantisera to allotypic specificities of hla-a and b antigens. serological characterization. | by using the lysostrip and the blocking of cytotoxicity by fab2 fragments, hla-a and b antibodies were detected in sera from rabbits and goats immunized with human high-density lipoprotein (hdl)-associated hla antigens isolated from sera and from cultured lymphoid cells. after appropriate absorptions, the xenoantisera became specific for hla-a and b alloantigens, reacted with the same structures as alloantisera, and displayed the same patterns of cross-reactions as alloantisera; however, several ... | 1979 | 92080 |
| species specificity in the immunologic reactions and biological functions of transcobalamin ii. | | 1979 | 92789 |
| penicillin and cephalosporin immunogenicity in man. | whether cross-allergenicity exists between the penicillin and cephalosporin antibiotics is controversial. a solid-phase radioimmunoassay was adapted to detect drug-specific igg, igm and ige antibodies. penicillin and cephalothin-specific serum immunoglobulins were determined in patients' serum before and after a therapeutic course of sodium cephalothin. changes in igg, igm and ige antipenicillin and anticephalothin antibodies, respectively, occurred and were closely correlated to one another (r ... | 1979 | 92902 |
| metabolism of l-[u-14c; 2,3-3h] valine in perfused goat mammary glands [proceedings]. | | 1979 | 92967 |
| binding of bovine immunoglobulins to pituitary intermediate lobe cells. | basophilic granular cells of the bovine pituitary intermediate lobe and intraglandular colloid show an affinity for immunoglobulin igg, iga, and igm. the cells were identified by direct and indirect fluorescent staining methods using blocking tests as controls. it is suggested that these cells may play some role in the synthesis and release of immunoglobulins or their determinants to the intact ig molecule. | 1979 | 93045 |
| are vh framework antigenic determinants expressed on both rabbit b and t lymphocytes? | | 1979 | 93052 |
| cross-reactions between serum proteins and water soluble liver tissue antigens of the nine-banded armadillo (dasypus novemcinctus linn.) and man. | cross-reactions between serum proteins and water soluble liver antigens of the nine-banded armadillo (dasypus novemcinctus linn.) and man were studied by crossed immunoelectrophoresis (cie). armadillo serum tested with rabbit antiserum against human serum proteins gave twelve components in cie. nine of these cross-reacting proteins were identified and showed partial identity with the corresponding human proteins. the electrophoretic mobility of alpha 2-macroglobulin and gc-globulin differed in t ... | 1979 | 93527 |
| rauscher leukemia virus-induced tumor antigens: complete separation from gp70, p30 and h-2. | the possibility that some or all of the viral proteins, gp70, p30, and the histocompatibility antigen, h-2, function as the tumor-specific transplantation antigen (tsta) of the r-mulv-induced leukemia, rbl-5, and also in the secondary in vitro induction of cytotoxic t lymphocytes (ctl), was investigated. the antigen was obtained by isolating the plasma membranes of rbl-5 cells and solubilizing with sodium deoxycholate (doc) followed by gel filtration chromatography. a fraction containing excelle ... | 1979 | 93583 |
| detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay. | a micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). the test is performed by coating wells of microtiter plates with purified hsv-specified glycoproteins, vp123 and vp119, derived from hsv-1- and hsv-2-infected cells, respectively. the coated wells are then incubated with diluted unadsorbed sera and subsequently with radioiodinated goat anti-human igg reagent. because these m ... | 1979 | 94044 |
| [specific soluble cell-wall antigens of "candida tropicalis" demonstrated by crossed immunoelectrophoresis (author's transl)]. | in the present study, soluble cytoplasmic extracts of candida albicans and c. tropicalis were prepared and showed respectively 67 and 47 precipitates in crossed immunoelectrophoresis performed with a commerical goat anti-c. albicans immunserum. purified cell walls of c. tropicalis have been prepared, and corresponding antiserum was got in rabbits. although important cross-reacting antigens exist between soluble cytoplasmic extracts of c. albicans and c. tropicalis, the c. tropicalis cell wall an ... | 1979 | 94248 |
| [comparative study of various chlamydia strains--preliminary study (author's transl)]. | | 1979 | 94564 |
| mouse mammary tumor virus (mmtv) antigen(s) are present on b lymphocytes of balb/c mice. | immunofluorescence studies using a polyvalent anti-mmtv serum revealed the presence of mmtv antigen(s) on the surface of spleen cells from the low mammary tumor incidence mouse strain balb/ccrgl. upon separation of the splenocytes on nylon-wool columns the populations of cells exhibiting positive fluorescence were nylon-adherent. these cells were considered to be b lymphocytes by the following criteria: presence of surface immunoglobulin (slg), absence of thy-1 antigens, enhanced responses to b- ... | 1979 | 94593 |
| clearance and glomerular localization of preformed dnp anti-dnp immune complexes. | the in vivo behaviour of well-defined immune complexes in rats was studied using complexes derived from dnp-conjugated bovine thyroglobulin (dnp-btg) and purified specific goat anti-dnp igg. both clearance and glomerular localization were mainly dependent on the nature of the antigen. soluble immune complexes formed with dnp17-btg were cleared faster and showed a more marked localization in the glomerular mesangium than complexes formed with dnp3.4-btg. a slight increase in the antibody to antig ... | 1979 | 94694 |
| laboratory characteristics of four strains of goatpox virus. | significant differences were found between four strains of goatpox virus isolated from goats in egypt, iraq, iran and the u.s.s.r., namely in their growth behaviour in embryonated eggs, ceiling temperature, plaque characteristics in cell culture, heat resistance and in the degree of inhibition of their replication by actinomycin d and bromodeoxyuridine. all the strains studied had morphologically similar virions and induced type a v- inclusions. they were uncapable to produce haemagglutinins in ... | 1979 | 94766 |
| antigenic homology of feline and human beta-hexosaminidase. | the immunological characteristics of feline beta-hexosaminidase (beta-d-n-acetylglucosaminidase, ec 3.2.1.30) isoenzymes, hex a and hex b, were studied. immunization of rabbits and goats with either cat hex a or hex b produced antibodies which reacted with a common antigenic marker shared by both hex a and hex b. with properly absorbed antisera, a unique antigenic marker was demonstrated on cat hex a, but not on hex b. this antigenic profile is comparable to that of the human beta-hexosaminidase ... | 1979 | 94833 |
| [the heterogeneity of streptokinases]. | purified streptokinases of some strains of different types of strep. pyrogenes (group a) as well as streptokinases of a strain of group c or group g streptococci, resp., were studied in regard of their heterogeneity. differences of the streptokinases were found in their amino acid compositions and in isoelectric points. serological differences were detected by a quantitative precipitation reaction with fluorescein-coupled antistreptokinase antisera from the goat as well as by neutralization reac ... | 1979 | 94885 |
| immunochemical comparison of human and goat haptoglobin and alpha 2-macroglobulin. | 1. a non-haptoglobin alpha 2-globulin was observed in the plasma of goats (capra hircus) after inflammatory stresses. 2. this stress reaction protein exhibited an electrophoretic mobility and molecular weight similar to goat haptoglobin. 3. complete immunological cross-reactivity was demonstrated between the goat protein and human alpha 2-macroglobulin. 4. the functional and evolutionary significance of these observations are discussed. | 1977 | 95679 |
| biochemical and serological characteristics of reverse transcriptase from human spleen in a case of childhood myelofibrotic syndrome. | | 1977 | 95799 |
| detection of antibodies to mycobacterium johnei by counterimmunoelectrophoresis. | counterimmunoelectrophoresis (cie) was applied in the detection of antibodies to mycobacterium johnei in 110 sheep, 11 goat and 31 cattle sera and compared to immunodiffusion (id) test. one per cent noble agar, 7 ml per slide of 5 cm x 10 cm; barbitone-tris buffer, mu = 0.03, ph 8.6; a constant current of 5 ma per slide and m johnei protoplasmic antigen at 4 mg per ml were found to impart high sensitivity to cie and give rapid results. cie detected 97 sheep, 11 goat and 31 cattle positive sera, ... | 1978 | 96579 |
| [study of the antigenic relationship between animal and human immunoglobulins using antisera to human immunoglobulins]. | cross-reactivity of monospecific antisera to human immunoglobulins with animal sera of 10 species was studied by immunoelectrophoresis and radial immunodiffusion. antisera to igg were shown to reveal igg of all the species studied, antisera to igm and especially to iga cross reacted less extensively. the greatest number of cross reactions were given by the antisera obtained as a result of hyperimmunization. hyperimmune monospecific antisera to human igg, iga, and igm can be used for the identifi ... | 1978 | 96633 |
| leukocytic endogenous mediator. | | 1978 | 97380 |
| a comparison of three serological tests in the diagnosis of caprine brucellosis. | the serum agglutination test (sat), the rose bengal plate test (rbpt) and the milk ring test (mrt) were used in the diagnosis of caprine brucellosis. there was a close correlation between the sat and rbpt when both tests were negative but the rbpt failed to detect 79.82 per cent of sera in excess of 50 iu. also, owing to the relatively poor milking potential of the nigerian goat and the false positive results with the mrt, it is concluded that the sat offers a better serological diagnostic tool ... | 1978 | 97740 |
| prostaglandins and regulation of the pulmonary circulation in the perinatal period. | effects of indomethacin on pulmonary vasodilation caused by ventilation of the fetal lungs with air were evaluated in anesthetized, exteriorized fetal goats by means of an open-chest pump perfused lung preparation. when fetal lungs are ventilated with air, pulmonary vascular resistance decreases in two steps: 1) a rapid decrease in pulmonary vascular resistance during the first 30 seconds of ventilation, and 2) a slower decline which continues through the first 10-20 minutes of more of ventilati ... | 1977 | 98094 |
| the fetus, pregnancy and parturition. | | 1977 | 98095 |
| differences between the discriminatory activity of antisera raised against the total gonadotropins and the pr-beta-hcg-tt for neutralization of hcg and lh action. | | 1978 | 98289 |
| isolation of immunoglobulin from splenic lymphocytes of inbred rats by triton x-100 solubilization. | | 1978 | 98589 |
| age-associated changes in the blood picture of the goat. | | 1978 | 98936 |
| incomplete inhibition of the immunoglobulin g-binding behavior of nitrated protein a. | nitration of protein a with tetranitromethane incompletely blocks its fc-binding ability for immunoglobulin g when measured by more sensitive methods of immunoprecipitation and when tested in the enzyme-linked immunosorbent assay. in addition, binding of protein a and blockage by nitration is heterogeneous and depends on the species origin of the immunoglobulin g. | 1978 | 99370 |
| early detection of pregnancy-associated serum protein using antiserum to placental antigens. | antisera against human placental proteins were developed in goats and rabbits, using immunoadjuvants and a prolonged injection schedule. the antisera were absorbed with normal serum proteins and then tested in immunodiffusion against normal and pregnancy sera. two bands of precipitation due to pregnancy antigens were observed in pregnancy sera as early as 18 days after conception. detection of these antigens has possibilities for application as an early pregnancy test. | 1976 | 59699 |
| isolation and preliminary characterization of two varieties of low molecular weight immunoglobulin in the bullfrog, rana catesbeiana. | two varieties of low m.w. immunoglobulins have been isolated from the serum of rana catesbeiana frogs. they are highly cross-reactive, although each also contains unique antigenic determinants. since both low m.w. immunoglobulins were identified in the serum of 22 individual frogs, it was concluded that they are isotypic variants. the light chains of r. catesbeiana and mammalian high and low m.w. immunoglobulins are similar in electrophoretic mobility on polyacrylamide gels containing sodium dod ... | 1976 | 59776 |
| cross reactivity of human beta2-microglobulin with human granulocyte colony-stimulating activity. | effects of antisera to human beta2-microglobulin (beta2 m) on factors able to stimulate colony formation in culture by human granulopoietic progenitor cells were investigated. the colony-stimulating activity (csa) present in media conditioned by cultures of human peripheral leukocytes was suppressed by treatment with anti-beta2m. this inhibition was not due to a direct effect on the granulopietic progenitor cells; controls to test for cytotoxicity and for noncytotoxic inhibition of the progenito ... | 1976 | 59777 |
| modulation of cyclic amp in purified rat mast cells. iii. studies on the effects of concanavalin a and anti-ige on cyclic amp concentrations during histamine release. | changes in rat mast cell cyclic adenosine 3',5' monophosphate (camp) concentrations during stimulation of histamine release by concanavalin a (con a) and anti-ige were studied. con a caused an increase in camp with a mean peak level at 20 sec of 232% of control (range 164% to 365%). con a-stimulated cells demonstrated falls toward control levels after 20 sec, but generally remained above control for at least 5 min. by 10 min camp had returned to control values. the con a effect on camp occurred ... | 1976 | 60447 |
| appearance, properties, and origin of altered human hemoglobin in feces. | altered hemoglobin (hb) has been found in the feces as a sequel to an upper gastrointestinal bleed. active hb antigen of increased anodic mobility was detected on immunoelectrophoresis of melena stools using a goat anti-hb. the hb derivative was also identified in polyacrylamide gel electrophoresis using 412 nm. absorbance. the alteration could be simulated in vitro by incubation of hemolysate with duodenal juice or purified carboxypeptidase b alone, or by a mixture of carboxypeptidases a and b. ... | 1976 | 60507 |