| identification of lymphoid cells in cultured of murine leukocytes and thymus. | several culture conditions and media were studied in an effort to establish long-term cultures of murine lymphoid cells from blood and thymus. cultures vessels included small glass bottles and rubber-stoppered tubes. media such as roswell park memorial institute 1640, 1700, 1701, 1715, gem 1717, nctc, fetal calf and horse serum supplements, and conditioned medium were tried. lymphoid cells in mouse leukocyte cultures survived as long as eight months before dying out. however, lymphoid cells in ... | 1975 | 46778 |
| inhibition by derivatives of phloretin of anaphylactic histamine release from human lung tissue and of prostaglandin f2alpha-induced bronchoconstriction. | derivatives of phloretin (25-1,000 mug/ml) among them polyphloretin phosphate (ppp), inhibited in a dose-dependent manner anaphylactic (birch pollen or horse dander) histamine release from human lung tissue passively sensitized with reaginic serum. pretreatment with ppp of lung tissue sensitized both to birch pollen and horse dander counteracted to a similar extent the release of histamine induced by either allergen administered in sequence. the phloretin derivatives also antagonized the constri ... | 1975 | 46848 |
| slow encephalopathies, inflammatory responses and arachis oil. | there is much interest in the possibility that diets rich in some plant oils may be of prophylactic and curative value in certain clinical conditions. this is generally attributed to the immunosuppressive effects of polyunsaturated fatty acids. arachis (peanut) oil injections can increase the incubation period of experimental scrapie in mice. in the late 1950s several workers showed that arachis and some other oils contained very potent anti-inflammatory components quite apart from the polyunsat ... | 1975 | 47424 |
| [equine infectious anemia in bulgaria]. | | 1975 | 47661 |
| [morphology and the morphological diagnosis of equine infectious anemia]. | | 1975 | 47662 |
| [synchronization of estrus in heifers by means of gestagenic preparations]. | studied were the synchronizing effect, the course of treatment, the combination of the gestagens cap and mga with pms as well as their effect on the conception rate in heifers. a total of 143 heifers of the bulgarian brown breed (89 test and 59 control), aged 17--18 months, weighing 350--360 kg on an average, were used in the experiment, divided into three test and three control groups. i test group. twenty-five animals were given daily one tablet of synchrorosyn-peach, the tablet containing 10 ... | 1975 | 48304 |
| inhibition by serum of encephalitogenic activity of myelin basic protein. | basic protein of myelin from bovine brain (b-bpm) in freund's complete adjuvant (fca) is highly encephalitogenic for the guinea pig. however, when b-bpm is mixed with serum from various species it loses its encephalitogenic effect but not its immunogenic properties. when the synthetic tryptophan peptide matching residue 115-126 of human bpm is mixed with normal human serum it loses both its encephalitogenic and immunogenic effects. the time of exposure to serum necessary for complete inhibition ... | 1975 | 48507 |
| group-specific component (gc) proteins bind vitamin d and 25-hydroxyvitamin d. | group-specific component (gc) proteins are human plasma proteins for which a worldwide polymorphism exists. as yet no functional role has been assigned this protein. we show that the products of both gc alleles, proteins gc 1 and gc 2 (distinguished electrophoretically), bind substantial quantities of vitamin d and 25-hydroxyvitamin d. three lines of evidence are reported: (1) polyacrylamide gel electrophoresis and autoradiography of serum labeled with (14-c)vitamin d3 revealed patterns of radio ... | 1975 | 49052 |
| observations on the t wave of the equine electrocardiogram. | the paper describes changes observed in the t wave and t vectorcardiogram in horse after various periods of exercise. using radiotelemetry and a bipole lead all horses showed negative t waves immediately after exercise. in some of them this was followed by a markedly positive t deflection. possible reasons for these changes are briefly discussed. | 1975 | 49267 |
| multiple specificities of mammalian blood group substances comparatively studied with human isoagglutinins and fractionated anti-h lectins. | purified blood group-active substances derived from different pig, horse, baboon, rhesus monkey and human tissues were quantitatively studied for their haemagglutination inhibiting potency with: (1) human igm anti-a and anti-b; (2) human anti-lea and anti-leb; (3) ulex europaeus extracts separated into lectin fractions with respective l-fucose-inhibitable ('anti-hf') and chitobiose-cellobiose-inhibitable ('anti-hc') combining sites. irrespective of species origin, a and b blood group activity pe ... | 1975 | 49294 |
| attenuation and immunogenicity of ts mutants of eastern encephalitis virus for mice. | preliminary experiments were intiated to test the attenuation for mice of chemically induced temperature-sensitive (ets) mutants of a virulents strain of eastern encephalitis (e) virus, and the potential of such mutants as live virus vaccines for mice. the reversion frequencies of eight mutants to temperature insensitivity were measured and the defects in their biosynthesis at the non-permissive temperature were studied. all eight mutants were less virulent for mice, but the extent of avirulence ... | 1975 | 49393 |
| t-mycoplasmas: a study of the morphology, ultrastructure and mode of division of some human strains. | the morphology of 10 strains of t-mycoplasma was studied in wet preparations of viable cells by darkfield, phase-contrast and interference microscopy, and in fixed preparations by various techniques of electron microscopy. mycoplasma-like artefacts in the horse-serum component of the medium were eliminated by filtration. all 10 strains were similar. individual cells were spherical, 0-25-1-0 mum in size, with a bounding trilaminar membrane, 10 nm thick and containing 7-5-12-5-nm particles, and a ... | 1975 | 49431 |
| drug-induced pseudolupus. | of fifteen patients with pseudolupus (a syndrome characterised by recurrent fever, myalgia, arthralgia, pleuritis, pulmonary infiltrates, pericarditis, myocarditis, and by mitochondrial antibodies in the absence of nuclear antibodies), all had been treated with "venocuran", one of a great number of drugs used for venous diseases. this drug, available in twenty countries under various names, contains phenopyrazone, horse-chestnut extract, and cardiac glycosides extracted from various plants. furt ... | 1975 | 49743 |
| letter: increased potency of killed vee (tc-83) vaccine in mice previously infected with eee virus. | | 1975 | 49960 |
| ventricular septal defect in a 7-year-old gelding. | | 1975 | 50309 |
| long-lasting in vitro immune response to a distinct antigenic determinant of a bacterial protein. cyclic changes of antibody titer and affinity. | long-lasting (60 days or more) antibody responses in vitro by rabbit lymph node fragments to a distinct determinant of escherichia coli beta-d-galactosidase were obtained by supplementing culture medium with fetal calf and horse serum. antibodies released in the supernatant were removed every 3rd to 5th day together with the spent medium, without pooling to minimize intermixing of molecules synthesized far apart in time. antibody titer, association constant, and heterogeneity index were measured ... | 1975 | 50342 |
| [does lead contribute to the pathogenesis of spine disease?]. | statistical data of a public insurance office of west germany show a drastic increase of the carriage impairments in juveniles. a plausible explanation of this phenomenon is presented by the lead quantities in auto exhaust. present levels of environmentall lead produce a measurable biochemical alteration in the population of industrialized countries (inhibition of the activity of the enzyme alad). by disturbing the synthesis of nucleics acids and porteins lead impairs fundamental processes of ... | 1975 | 50674 |
| presence of "receptors" for growth hormone on membranes of rat thymocytes. | growth hormone conjugated to sepharose-4 b was found to mimic the action of the free hormone in the stimulation of the uptake and incorporation of radioactive uridine into rna in thymocytes in vitro. the integrity of the cellular structure was essential for the stimulating effect of the hormone. the bonding of the immunologically reactive determinants of the hormone on membranes of thymocytes was demonstrated by electron micrographs of preparations incubated sequentially with rabbit antigrowth ... | 1975 | 50701 |
| the interaction of concanavalin a and the surface coat of stratified squamous epithelium. | the interaction of concanavalin a (con a) and the surface membranes of the epithelial cells of human oral mucosa, epidermis and guinea-pig lip was studied. two methods were used: fluorescent con a and con a in combination with horse radish peroxidase. with both methods the surface coat of epithelial cells was stained. the specificity of the staining methods could be demonstrated. the interaction between pemphigus antibodies and the surface membranes of epithelial cells could be blocked by con a. | 1975 | 50858 |
| hormones in canine gynecology. a review. | | 1975 | 51807 |
| the potentiality of antibody-producing cells. ii. evidence for two antibody molecules of different specificities secreted by micromanipulated bispecific mouse spleen cells. | bispecific pfc appearing on the 4th day after immunization of mice with sheep erythrocytes conjugated with trinitrophenyl hapten (tnp-srbc) were identified by their capacity to lyse native srbc and tnp-conjugated horse erythrocytes (tnp--horbc) simultaneously in an open carboxymethylcellulose medium. individual pfc thus detected, were micromanipulated into two successive media containing the indicators srbc and tnp--horbc. out of 103 transferred double cells ninety-two (89 per cent) remained dou ... | 1975 | 52617 |
| antigenic relationship between the surface antigens of avian and equine influenze viruses. | influenza virus equine 1 (a/equine/prague/56) has a hemagglutinin which is antigenically related to the hemagglutinin of fowl plague virus strain rostock (fpv) and a neuraminidase which cross-reacts with the enzyme of virus n (a/chick/germany/49). after a single injection of chickens with equine 1 virus no hemagglutination inhibiting (hi) and neutralizing antibodies against fpv can be demonstrated, although the birds are fully protected against a lethal dose of fpv. hi and neutralizing antibodie ... | 1975 | 53781 |
| [potency determination of horse anti-human-lymphocyte serum (als) by means of the lymphocyte resistance test, a modification of schroder's leukocyte resistance test]. | the leukocyte resistance test of schröder was used for the purpose of modifying the in vitro determination of cytotoxic potency of als during the course of immunization. in contrast to the original method, where the test was carried out with whole blood, the incubation was made with pure lymphocyte cell suspensions. the results obtained show that the extensive procedure of examination enables the cytotoxic effectiveness of antilymphocytic antibodies to be evaluated. | 1975 | 54295 |
| quantitative assay of diphtherial toxin and of immunologically cross-reacting proteins by reversed passive hemagglutination. | a reversed passive hemagglutination (rpha) assay for diptherial toxin has been developed. antitoxic antibodies were isolated from commercially available equine diptherial antitoxin by immunoabsorption using highly purified diphtherial toxin covalently linked to sepharose 4b. formalinized, tanned sheep erythrocytes sensitized with the purified antitoxic antibodies are specifically agglutinated by diphtherial toxin but are not agglutinated by extracellular antigens of corynebacterium diptheriae th ... | 1975 | 54339 |
| [determination of arbovirus group affiliation in the indirect hemagglutination reaction]. | a dry polyvalent erythrocyte immunoglobulin diagnostic preparation for arboviruses of group b may be prepared to be used in the indirect hemagglutination test. the preparation detects antigens of arboviruses of this group in the tissue culture fluid and in suckling mouse brain tissue treated with sucrose-acetone. the test with group b arbovirus antigens is inhibited specifically by the homologous polyvalent immune gamma globulin. inhibition of hemagglutination by species-specific immune globulin ... | 1975 | 54999 |
| immunologic release of histamine from bovine polymorphonuclear leukocytes. | blood from calves sensitized to horse serum was fractionated to yield a suspension containing 94% granulocytes. these cells released up to 90% of their total histamine when exposed to the antigen. this is a noncytotoxic reaction similar to that observed in human beings, rabbits, and guinea pigs. higher concentrations of antigen caused inhibition of histamine release. this immunologic reaction may be important in bovine hypersensitivity, especially in the lungs, where intravascular aggregations o ... | 1975 | 55087 |
| [dry erythrocytic diagnostic agent for the determination of antiglobulins]. | dry erythrocytic diagnostic agents were obtained under experimental conditions for determination of antiglobulins forming in the organism of man and animals under the effect of serum preparations from the blood of horses and homologoum immunoglobulins. a study was made of the sera of 100 patients with tick-borne encephalitis treated with heterologous and homologous immunoglobulins of directed action; in response to the administration of horse gamma-globulin antiglobulins (in titres below 1 : 100 ... | 1975 | 56112 |
| antigenic and morphologic comparisons of ibaraki and bluetongue viruses. | bluetongue disease virus, type 10, and ibaraki disease virus, which causes a bluetongue-like disease of cattle, were compared to determine whether they are the same or different viruses. they were similar in morphology, but neutralization tests, complement-fixation tests, and ferritin tagging indicated that they have antigenic differences. therefore, they should be considered as different viruses. two other viruses of this group, african horsesickness and equine encephalosis, were included to sh ... | 1975 | 56211 |
| a long-term comparative study of the mammalian larynx, based on whole organ serial sectioning. | the paper includes brief details of a long term three-dimensional study into the fine structure of the mammalian larynx using whole organ serial sections. differential staining, including glees silver technique, is essential if maximum information is to be gained. a specially designed punch card will enable any future investigator to rapidly retrieve relevant information and it is expected that specimens of all available mammalian larynges will be included. illustrations are included in this pap ... | 1976 | 57693 |
| cardiac monitoring during exercise tests in the horse. 3. changes in the electrocardiogram during and after exercise. | changes that occur in the equine ecg during and after exercise have been described and compared with resting ecg's obtained from the same horses. when the speed of work equals or is greater than "three-quarter pace" (i.e. "even time" or 200 metres in 15 seconds) the high heart rates developed cause the loss of ecg waveforms that are readily discernible at rest. although differences in the waveforms between horses with normal and abnormal resting ecg's have been observed, the more definitive usef ... | 1976 | 57770 |
| specific in vitro lymphocyte transformation with venezuelan equine encephalitis virus. | | 1976 | 57835 |
| suppression of antibody responses in allogeneic mice by products of lymphoid tissue. ii. lack of antigenic specificity and immunogenetic requirements of allogeneic suppressive factor (asf). | mice were irradiated, infused with thymocytes and immunized with a variety of antigens, i.e., sheep or horse red blood cells (srbc or hrbc), diphtheria toxoid (dt) or bovine gamma-globulin (bgg). the spleen cells (t.spleen cells) were harvested 5 days later and cellfree extracts were prepared. the extracts contained an allogeneic suppressive factor (asf) that was capable of inhibiting igm antibody responses of allogeneic or semi-allogeneic unirradiated mice. asf had to be injected within 24 hr o ... | 1976 | 58047 |
| variations in the susceptiblility of field and laboratory populations of culex tarsalis to experimental infection with western equine encephalomyelitis virus. | four field populations and four laboratory colonies of culex tarsalis from california were evaluated for their susceptibility to infection with western equine encephalomyelitis (wee) virus by intrathoracic inoculation and by feeding on pledgets soaked with a virus-blood-sucrose mixture. all mosquito strains were uniformly susceptible to infection by intrathoracic inoculation, but 50% infective doses for field and colonized strains varied as much as 1000- to 1500-fold, respectively, by pledget fe ... | 1976 | 58557 |
| antithymocyte globulin in renal transplant recipients. report of a prospective randomized controlled trial. | horse antihuman thymocyte globulin (hahtg) combined with prednisone and azathioprine (lmuran) was used as immunosuppressive therapy in a randomized controlled sutdy in 50 renal allograft recipients. side effects of hahtg administration given intravenously were mostly mild. in the treated group, four patients out of 26 died of infectious complications, whereas in the control group, three patients out of 24 died of infectious complications (chi2 = .01,p greater than .05). the graft survival at 18 ... | 1976 | 58651 |
| morphological components of herpesvirus. ii. preservation of virus during negative staining procedures. | the disruption of envelopes and the fragmentation of capsids of equine herpes-virus type i observed in negatively stained samples were attributed to viral dehydration on carbon films during preparation for electron microscopy. prior fixation of virus with oso4 or glutaraldehyde and subsequent application of negative stain before drying minimized envelope disruption and virtually eliminated the occurrence of capsomere sheets and broken capsids. this sample procedure significantly improves electro ... | 1976 | 58962 |
| chemical mediators of anaphylaxis (histamine, 5-ht, and srs-a) released from horse lung and leukocytes in vitro. | horses were sensitized to bovine plasma in freund's complete adjuvant. leukocytes, separated from venous blood, yielded histamine upon incubation with bovine plasma. ioslated lung fragments incubated with bovine plasma liberated histamine and 5-ht, but not srs-a. pulmonary veins obtained from the same animals contracted to histamine, 5-ht and to antigen (schultz-dale reaction). histamine and 5-ht probably contribute to immediate-type hypersensitivity in horses whereas the role of srs-a is not pr ... | 1976 | 59377 |
| the effect of neuraminidase on the sensitivity of tumor cells toward lysis by antibody and complement or by sensitized lymphocytes. | the effect of neuraminidase treatment on the antigenicity of sarcoma i (sal) and mastocytoma p-815-x2 cells was compared, both in terms of their reaction with various antisera (c57bl/6 anti-sai, c57bl/6 anti-mastocytoma, rabbit and horse als) and complements (guinea pig, rabbit) or with sensitized lymphocytes (c57bl/6 anti-sai, c57bl/6 anti-mastocytoma, a anti-mastocytoma). with sensitized lymphocytes the reactivity of neuraminidase-treated tumor cells was similar to that of nontreated cells. in ... | 1976 | 59438 |
| serological studies and isolations of serotype hardjo and leptospira biflexa strains from horses of argentina. | three pathogenic leptosipras and 12 saprophytic leptospira biflexa strains were isolated from 72 apparently normal horse kidneys collected at an abattoir in argentina. cross-agglutination reaction patterns of the pathogens showed that they were antigenically homologous with members of the hebdomadis group. when one of the strains was compared to hebdomadis serotypes in reciprocal agglutination-absorption tests, it was found to be serologically homologous to serotype hardjo. this is the first kno ... | 1976 | 59735 |
| [fab immunoglobulin fragments. i. the comparative characteristics of the serological and virus-neutralizing properties of a gamma globulin against tick-borne encephalitis and of the fab fragments isolated from it]. | a comparative study was made of the serological properties and virus-neutralizing activity of antiencephalitis gamma-globulin and fab-fragments isolated from it by gel-filtration. horse immunoglobulins against the autumno-summer tick-borne encephalitis virus could be disintegrated with the aid of papaine to monovalent fab-fragments which (according to the complement fixation reaction, the test of suppression of the complement fixation, and the hait) retained the serological activity whose level ... | 1976 | 60019 |
| production of an equine anti-bovine leukocyte serum. | a method is described for production of an equine anti-bovine leukocyte serum (eabls). leukocytes were harvested from the milk of cow's udders which had been irritated with endotoxin. the washed leukocytes as antigens were administered to 2 horses in a series of subcutaneous and intravenous injections. there was a variably progressive increase in total serum proteins and a decrease in albumin/blobulin ratios, but the most pronounced change was an increase in beta2-globulins. accompanying these c ... | 1976 | 60071 |
| [contribution to the antigenic study of influenza viruses in animals. i.--neuraminidase of the equine influenza viruses (author's transl)]. | from the revised nomenclature of who, the fowl influenza virus a/duck/ukraine/63 (hav7 neq2) has the same neuraminidase as the equine virus a/equi 2/miami/63 (heq2 neq2); the a/chicken germany "n"/49 virus has the same neuraminidase as the equine virus a/equi 1/prague/56. a comparative study of the antigenic specificities confirms that the neq2 neuraminidases are closely connected, whatever their animal origin, and that the fowl strain hav7 neq2 can be used for the titration of anti neq2 antibod ... | 1975 | 60076 |
| inhibition by 5-hydroxytryptamine of anaphylactic histamine release from bovine granulocytes. | a suspension of granulocytes from calves sensitised to horse plasma released histamine when exposed to the antigen. histamine release was strongly inhibited by 5-hydroxytryptamine in a dose-related fashion. 5-ht is known to be released from bovine lungs in vitro, and may regulate the release of histamine in vivo during anaphylaxis. | 1976 | 60245 |
| an electromyographic study of uterotubal activity in the ewe. | the electrical activity of the oviduct and uterus was automatically registered on a potentiometric pen recorder in ewes fitted with an extracellular multielectrode assembly. the duration and amplitude of local non-propagating activity of the uterus increased and became progressively grouped into phases as natural oestrus progressed. phases of contractions lasting 5-6 min were initially propagated at a rate of 2/hr. their frequency increased within 24 hr of the decline in plasma progesterone leve ... | 1976 | 60491 |
| development of immuneeoglobulin and antibody-forming cells in different stages of the immun response. | after primary immunization of mice, rats and rabbits with antigens (horse radish peroxidase, bovine serum albumin and muchroom tyrosinase) emulsified in complete or incomplete freund's adjuvant, both cells synthesizing immunoglobulin without detectable antibody function and antibody-producing cells were detected. the first cells which appeared were synthesizing and secreting igg and igm immunoglobulins without antibody function. these cells were progressively replaced by cells synthesizing and s ... | 1976 | 60908 |
| equine infectious anemia virus: evidence favoring classification as a retravirus. | equine infectious anemia virus (eiav) has a density of 1.154 g/cm3 in sucrose a high-molecular-weight rna similar in size to rauscher murine leukemia virus, and an internal virion reverse transcriptase that utilizes the synthetic rna template poly(ra) but not the synthetic dna template poly(da), both with (dt)12 as primer. although capable of utilizing manganese at low concentrations (approximately 0.1 mm), eiav reverse transcriptase showed highest activity in the presence of 9 mm magnesium. the ... | 1976 | 61283 |
| cumulation and elimination of horse-anti-dog lymphocyte and normal horse gammaglobulin in dogs. | two groups of dogs received daily intravenous doses of 20 mg/kg 131-i-labelled horse-anti-dog lymphocyte globulin or normal horse gammaglobulin respectively over a period of 11 days. horse-anti-dog lymphocyte globulin showed a significantly higher eleimination rate than normal horse gammaglobulin. in contrary to the continuous increase in serum radioactivity during normal horse gammaglobulin treatment, there was a plateau after the 5th day in the horse-anti-dog lymphocyte globulin group. the xen ... | 1976 | 62375 |
| detection of immunologically active zones in equine growth hormone. | peptide fragments, obtained from equine growth hormone by cyanogen bromide cleavage and further chemical treatment, were isolated and identified. their immunological reactivities were tested by hemagglutination and complement fixation methods using rabbit antisera against native hormone. antigenic determinants were detected in the fragments comprising amino acid sequences 5-72 and 73-123, this last one being predominant. fragment 124-178 had very low reactivity. nitration of peptide 73-123 did n ... | 1976 | 62669 |
| study of human t and b lymphocytes with heterologous antisera. ii. partial characterization of a human t lymphocyte antigen. | using a horse anti-human thymocyte serum made specific for t lymphocytes by absorption (anti-htla serum), a material reacting with it has been extracted from detergent-lysed peripheral blood lymphocytes. acid elution and gel-filtration analysis allowed recovery of two peaks containing htla activity: one of 30,000-40,000 daltons, the other being excluded by sephadex g-200 and likely to be more than 300,000 daltons. htla appeared to be released by lymphocytes incubated at 37 degrees, but not at 4 ... | 1976 | 62713 |
| absence of thymus-derived lymphocyte markers in myelogenous leukemia (ph1+) cell line k-562. | the myelogenous leukemia cell line k-562 with a ph1+chromosome, derived from a patient with chronic myelogenous leukemia in terminal blastic crisis, is not a bone marrow-derived lymphoblastic cell line, because the cells neither produce immunoglobulins nor possess complement receptors. since it has been suspected that blasts found in some patients with chronic myelogenous leukemia in blastic crisis might be thymus-derived cells, we have studied several parameters to demonstrate that k-562 cells ... | 1976 | 63324 |
| rocking-horse paediatric weighing-machine. | | 1976 | 63854 |
| [preparative isolation of alpha 2-macroglobulin, transferrin, albumin and study of their nonspecific gamma-inhibitory activity]. | profiles of distribution of non-specific gamma-inhibitors of influenza a2/victoria/35/72 in donkey and horse sera were established by gel chromatography in sephadex g-200. high and low molecular inhibitors were found in 19s and 4s serum fractions. highly purified preparations of a2-macroglobulin, transferrine and albumin were isolated by a combination of methods of salt precipitation, gel chromatography on sephadex g-100, g-200 and ion exchange on deae-sephadex a-50. heating sera resulted in a c ... | 1976 | 64024 |
| iron absorption in gastric and duodenal pathology in patients with iron deficiency anaemias. | absorption of radioactive iron was studied in 87 patients with different types of iron deficiency anaemias and in 23 healthy subjects. the subjects were given 1...2muci of radiactive iron in the form of feso4 together with 5 mg of nonradioactive iron as a carrier and 100 to 150 g of white bread, radioactivity on the whole body being studied with a big liquid scintillation counter 4 pi (blsc-2). in clinical observations and in single experiments on volunteers there was no conformity of the values ... | 1976 | 64433 |
| cardiac arrhythmias in the horse. | electrocardiograms were obtained from normal horses and from horses with cardiac or other organic disease that affected the cardiac rhythm. tracings were obtained from a base-apex bipolar monitor lead, with the negative electrode attached to the skin in the right jugular furrow and the positive electrode attached to the skin on the ventral medline, beneath the apex of the heart. each arrhythmia was discussed relative to importance and probable cause. | 1977 | 64466 |
| virus-replicating t cells in the immune response of mice. i. virus plaque assay of the lymphocytes reactive to sheep erythrocytes. | virus plaque-forming cell assay with vesicular stomatitis virus (vsv), which had been originally introduced by bloom and his colleagues as a tool for the enumeration of activated lymphocytes, was first applied to the immune response of mice to a widely used antigen, i.e. sheep red blood cells (srbc). when spleen cells taken from mice previously primed with srbc were cultured in the presence of the antigen, lymphocytes capable of replicating vsv (antigen-induced virus plaque-forming cells, ag-v-p ... | 1977 | 64584 |
| [avidity criteria in assessing the functional activity of antigens, antibodies and non-specific serum inhibitors on a model of the kinetic reaction of hemagglutination suppression with arboviruses]. | the functional activity of some arboviruses of groups a and b, of the antibodies and serum inhibitors was studied on a model of the kinetic hemagglutination inhibition test (hai) by different avidity criteria (velocity, completeness and stability of formation of a neutral complex). the avidity indices of the antigens, antibodies and the inhibitors proved to depend on the group, species and strain peculiarities of the arboviruses, the method of preparation of the antigen, the biological species o ... | 1976 | 65077 |
| effect of d-glucosamine on the growth pattern of rna viruses [proceedings]. | | 1976 | 65993 |
| interaction of mouse peritoneal macrophages with different arboviruses in vitro [proceedings]. | | 1976 | 66026 |
| relationship between e receptors and a t-specific surface antigen on human t cells. | the aim of this study was the delineation of different antigenic surface determinants on the surface of adult peripheral t cells by means of a specific horse anti-human t-cell serum (ats). it was shown that this serum reacts both with e receptors and (an) additional t antigen(s). while e receptors showed the already known susceptibility to trypsin, t antigens (as demonstrated in cytotoxicity tests) were resistant to trypsinization even at high concentration. incubation of the trypsinized periphe ... | 1977 | 66193 |
| oncogenicity in marmosets of hl-23v, a type c oncornavirus isolated from human leukemic cells, and comparison with simian sarcoma virus type 1 (ssv-1/ssav-1). | type c virus produced by dog thymus cells (a7573) that were infected with virus (hl-23v), isolated from cultured leukocytes of an acute myelogenous leukemia patient, transformed marmoset and horse cells in vitro and induced virus-producing fibromas in marmosets. the tumors and transformed foci were indistinguishable morphologically from those induced by simian sarcoma virus, type 1 (ssv-1/ssav-1). hl-23v was indistinguishable from ssv-1/ssav-1 by immunofluorescence and neutralization tests, and ... | 1977 | 66319 |
| studies on an adrenal antigen common to man and different animals. | precipitating adrenal antibodies, originally described by andrada et al., are often associated with patients suffering from the moniliasis-polyendocrinopathy syndrome. the syndrome may include contemporarily several organ-specific autoimmune diseases such as hypoparathyroidism. addison's disease, thyroiditis, pernicious anaemia, gastritis and ovarian failure and often combined with moniliasis and alopecia. these antibodies seem to differ from those demonstrable by immunofluorescence (ifl) and co ... | 1977 | 66422 |
| [ultrastructural identifaction of human t lymphocytes using a peroxidase-conjugated anti-htla serum]. | a horse antiserum rendered specific for human t lymphocytes was conjugated with peroxidase (po) and used for identification of tonsilar t lymphocytes in electron microscopy. this conjugate stained nearly 50% of tonsilar lymphocytes, 96% of the t of the t cell-enriched population and 4% of the b cell-enriched population. the staining was only found on the cytoplasmic membrane as irregular small spots all over the cell surface. this conjugate was used for identification of the lymphocyte sub-popul ... | 1977 | 66907 |
| a unique property of fetal bovine serum: high levels of protein-glutathione mixed disulfides. | fetal bovine serum has been reported to delay or inhibit "spontaneous" neoplastic transformation in vitro as compared with all other sera tested. the present results indicate that fetal bovine serum is also unique in containing high levels of protein-glutathione mixed disulfides (3 to 7 microng glutathione as mixed disulfide per ml serum). the level of mixed disulfide appears to vary in accordance with the period of gestation of the fetal calves used to prepare the serum, decreasing below detect ... | 1977 | 67079 |
| physicochemical characterization of two serologically unrelated equine rhinoviruses. | the physicochemical properties of two serologically distinct equine rhinoviruses have been examined. each virus sedimented at approximately 160s but co-centrifugation of the two viruses in a sucrose gradient revealed a small difference in their sedimentation coefficients. the two viruses also have different buoyant densities in cesium chloride. the equine rhinovirus type 1 equilibrated as a sharp peak at 1.45 g/ml whereas the type 2 virus equilibrated as a heterogeneous band with a peak at 1.44 ... | 1977 | 67105 |
| rna-dependent dna polymerase associated with equine infectious anemia virus. | equine infectious anemia (eiav) is shown to have an associated rna-instructed dna polymerase similar in its cofactor requirements and reaction conditions to the rna tumor virus dna polymerases. demonstrating this dna polymerase activity requires a critical concentration of a nonionic detergent, all four deoxyribonucleoside triphosphates, and a divalent metal ion. the reaction is sensitive to rnase, and a substantial fraction of the fna synthesized is complementary to viral rna. the detection of ... | 1977 | 67219 |
| intrathecal antitetanus serum (horse) in the treatment of tetanus. | in a two-year study of 322 conservatively treated, consecutive cases of tetanus in a rural hospital (all over twelve months old), intrathecal administration of 200 units of antitetanus serum (a.t.s.) (horse) reduced the overall mortality of 4-5% (5/110) compared with 14-5% (16/111) in the control series. 200 units intrathecal a.t.s. (horse) gave better results than 1500 units a.t.s. (horse). the results with lumbar and cisternal administration did not differ. it is suggested that tetanus is a po ... | 1977 | 67468 |
| a heterophile carbohydrate moiety common to mammalian igm and erythrocytes detected by chicken igm antibody. | sequential treatment of chicken lymphocytes with normal sheep serum (nss), chicken antiserum against sheep erythrocytes (se) and formaldehyde results in rosette formation with se. the identity of reactive components and the mechanism of rosette formation has been elucidated. the binding activity of nss to chicken lymphocytes was ascribed to "natural" igm anti-species antibodies. subsequently, chicken igm anti-se antibody reacted with sheep igm bound to the lymphocyte surface, and, following form ... | 1977 | 67955 |
| the effect of heterologous antisera on embryonic development. xiii. lack of effect of antisera to alpha fetoprotein. | this investigation was carried out to determine whether heterologous antisera to alpha fetoprotein (afp) are embryotoxic to developing rat embryos. homogeneous rat afp was isolated and antisera directed against this glycoprotein were produced in rabbits, horse and goat. the effect of the antisera on embryonic development was examined by injecting the antisera intraperitoneally into pregnant rats on the ninth, eleventh and thirteenth days of gestation. the results demonstrated that there was no e ... | 1977 | 68673 |
| estrus, ovulation and conception following timed insemination in romney ewes treated with progestagen and gonadotropins. | | 1977 | 68895 |
| antigenic relationships among thiol-activated cytolysins. | cereolysin, streptolysin o, and perfringolysin o formed precipitin lines that completely fused when reacted with horse antitetanolysin by ouchterlony immunodiffusion and formed precipitin lines that showed either partial or complete fusion when diffused against horse antistreptolysin o or antiperfringolysin o. | 1977 | 68932 |
| carrier-directed anti-hapten responses by b-cell subsets. | the capacity of the trinitrophenyl (tnp) haptenic group, coupled to a series of chemically dissimilar carriers, to cross-stimulate putative t- dependent and t-independent murine b-cell subpepulations was determined by using an in vitro limiting dilution technique to generate primary igm responses. it was found that tnp-ficoll and tnp-dextran, two t- independent antigens with little or no polyclonal mitogenicity, stimulate the same population of anti-tnp precursors, which is distinct from the pre ... | 1977 | 68986 |
| purification of equine infectious anemia virus antigen by affinity chromatography. | affinity chromatography was performed to obtain highly purified antigen from equine infectious anemia (eia) virus. after crude antigen was concentrated by polyethylene glycol precipitation of culture fluids from equine dermal cells persistently infected with eia virus, and after the virus was disrupted with ether, it was added to a column of cyanogen bromide-activated sepharose 4b to which eia-specific antibody had been conjugated. the antigen was effectively released from the column with 5m mgc ... | 1977 | 69631 |
| improvement of fertility in post-partum beef cows by treatment with pmsg and progestagen. | | 1977 | 69709 |
| characterization of a retravirus isolated from squirrel monkeys. | a new retravirus (smrv) isolated from a squirrel monkey, saimiri sciureus, has an mg2+-dependen reverse transcriptase and a buoyant density of 1.17 g/cm3 in sucrose and 1.21 g/cm3 in cesium chloride, similar to the mouse mammary tumor virus and the mason-pfizer monkey virus. the polypeptide patter of smrv as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was distinct from the reported polypeptide patterns of known retraviruses. four major polypeptides of molecular weight ... | 1977 | 69728 |
| preparation of anti-cea and anti-goat gamma-globulin sera for radioimmunologic assay of carcinoembryonic antigen. | goats were immunized with purified carcinoembryonic antigen, and the suitability of the antisera for clinical assays of carcinoembryonic antigen was characterized. reactivity of equine sera to goat gamma-globulin as a precipitating factor in the radioimmunologic double antibody technique was also evaluated. | 1977 | 70197 |
| immunogenic contaminants in mouse nerve-growth factor. | experiments are described that confirm the presence of gamma-globulin in standard preparations of mouse nerve growth factor (7-s complex and beta subunit) and show that antibodies to this protein are present in horse antisera to the growth factor. these antibodies may be partially removed by adsorption with soluble antigen and totally removed by affinity chromatography on columns of insolubilised antigen. these procedures do not affect the potency of the antiserum in vitro or in vivo. contaminat ... | 1977 | 70351 |
| antibody response to horse gamma-globulin in recipients of renal allografts: relationship with transplant crises and transplant survival. | anti-antilymphocyte globulin (alg) antibody response was measured every day during and after alg treatment in 52 recipients of renal allografts. igm antibodies became detectable in 37 patients, usually at day 8 and igg antibodies appeared 3 days after the igm in 21 of 37 cases. of 30 transplant crises recorded between days 6 and 11, 20 coincided with the onset of the antibody response, and the incidence of crises during this period was higher among antibody producers than among nonproducers. in ... | 1977 | 70872 |
| characterization of extract of dog hair and dandruff from six different dog breeds by quantitative immunoelectrophoresis. identification of allergens by crossed radioimmunoelectrophoresis (crie). | an extract of mixed dog hair and dandruff from six different dog breeds (alsatian, boxer, collie, poodle, and long-haired and short-haired dachshund) was obtained by mild extraction, centrifugation, dialysis and freeze-drying. extract of hair and dandruff from the individual dog breeds was obtained in the same way, but the material was not freeze-dried. examination and characterization of the mixed extract by means of crossed immunoelectrophoresis revealed a precipitation pattern composed of 25 ... | 1977 | 70943 |
| structural studies on horse spleen apoferritin. | | 1976 | 71051 |
| methyl alcohol poisoning. iv. alterations of the morphological findings of the retina and optic nerve. | the ocular morphological findings of three methyl alcohol-intoxicated rhesus monkeys with optic disc swelling was investigated with light and electron microscopy in conjunction with intravascular horse radish peroxidase. alterations observed in the optic nerve head were confined to the axons and consisted of swelling and clustering of the mitochondria, disruption of the neurotubules, the formation of vesicles, and enlargement of the axon segments in the prelaminar region. swelling of the oligode ... | 1977 | 71893 |
| anaphylactoid reactions due to non-immune complex serum protein aggregates. | the infusion of aggregate-containing i.v. human gamma-globulin as well as human serum albumin can lead to severe anaphylactoid reactions with decrease in mean arterial pressure, increase in pulmonary artery pressure and decrease in cardiac output in unsensitized dogs, while the deaggregated solutions are well tolerated. during these anaphylactoid reactions, no significant changes in the serum complement activity of the dogs were observed. in clinical human serum albumin incompatibility, stimulat ... | 1977 | 72353 |
| [use of suspension adsorption methods for studying arboviruses]. | hemagglutinating antigens of western equine encephalomyelitis (wee) virus and 5 strains of tick-borne encephalitis (tbe) virus were charcaterized by their capacity to adsorb on inorganic (kaolin, bentonite, barium sulphate) and organic (chick embryo fibroblasts) adsorbents. the adsorption capacity of tbe virus was more marked than that of wee. the tbe virus strains 4072 and 1524 avid in serological tests adsorbed better on inorganic adsorbents than nonavid strains no. 80 and 1509. in contrast, n ... | 1977 | 72456 |
| cytochemical staining of equine blood and bone marrow cells. | | 1977 | 72660 |
| [fluorescence microscopic demonstration of eosinophile leucocytes with different fluorochromes (author's transl)]. | a screening program involving blood from men and horse resulted in about 80 fluorochromes admitting microscopic differentiation of eosinophile leucocytes (properly their granules) in blood smears. fluorescence distinguishes either selective, i. e. only eosinophile cells show fluorescence, or specific, that means, eosinophilic granules exhibit fluorescence differing from non-eosinophilic cells by colour and/or intensity. | 1977 | 73340 |
| histamine enhances anaphylactic histamine release from bovine lung and leukocytes via histamine h2-receptor. | chopped lung and isolated leukocytes of calves sensitized to horse plasma release histamine when incubated with the antigen. low concentrations (10(-9) and 10(-8) m) of exogenous histamine enhanced both spontaneous and antigen-induced histamine release. the enhancement of antigen-induced histamine release was inhibited by metiamide but not by mepyramine, whereas the enhancement of spontaneous histamine release was not significantly affected by either histamine antagonist. at higher concentration ... | 1978 | 73583 |
| aplastic anaemia: evidence for an immunological mechanism. | the soft agar culture assay (c.f.u.-c) has been used in vitro as a measure of haemopoietic capacity of bone-marrow. in a patient with aplastic anaemia pretreatment of the patient's bone-marrow with horse anti-human-thymocyte globulin and complement (a.t.g. + c) prior to culture led to a dramatic increase in ability to form colonies in the soft agar assay; and co-culturing marrow from a normal donor and from the patient resulted in a distinct reduction in the number of expected c.f.u.-c. these fi ... | 1976 | 73642 |
| histochemistry of the duodenal glands of the cat and horse. | the duodenal glands of cat and horse were studied using pas, alcian blue, dialysed iron, aldehyde fuchsin-alcian blue and high iron diamine stains. it was found that the duodenal glands of the horse reacted positively to alcian blue, dialysed iron stains and also took the alcian blue stain in the combined aldehyde fuchsin-alcian blue and high iron diamine-alcian blue stains. those of the cat gave negative results. these results suggest the presence of acidic groups in the mucosubstances secreted ... | 1976 | 74185 |
| studies on equine immunoglobulins--v. horse antibodies to donkey igga. | | 1977 | 74358 |
| increase of immunoglobulin t concentration in ponies as a response to experimental infection with the nematode strongylus vulgaris. | | 1978 | 75703 |
| antigenic relatedness of equine herpes virus types 1 and 3. | antiserums prepared in specific pathogen free (spf) ponies were used in direct and indirect immunofluorescence, immunodiffusion, complement fixation and serum neutralization procedures to study the interrelationships of the three types of equine herpes viruses (ehv-1, ehv-2, and ehv-3). equine cell cultures infected with each type virus fluoresced when stained with homologous conjugated antiserum. in reciprocal tests ehv-1 and ehv-3 cross-fluoresced, but ehv-2 did not cross-fluoresce. non-infect ... | 1978 | 75724 |
| special topics in clinical pathology. | the following topics are discussed in this presentation: a. recent advances in the use and interpretation and methodology of antibiotic susceptibility testing. b. improvements in sample submittal to obtain accurate results from your laboratory. c. staining blood, bone marrow, and cytology specimens in the office laboratory. d. pathogenesis and differential diagnosis of lipemia. e. differential diagnosis of abdominal effusions. | 1978 | 75783 |
| [antigen-elimination technic. an in vivo method for studies of the human immune response against horse gamma globulin]. | in 20 patients treated with antilymphocyte horse gamma-globulin (alg) the immune response was tested by means of the antigen elimination technique before, during and after alg-therapy. the comparison of this method with agargel diffusion test, indirect hemagglutination and skin tests showed that immunization of patients against horse protein is indicated earlier and more reliable with the antigen elimination. therefore this technique should be utilized during a therapy with foreign proteins espe ... | 1978 | 75829 |
| specificity of helper t cells for different antigens. | balb/c nude mice have been injected with 10(6) congenic thymus cells, a number which allows some, but not all mice to respond to any particular t-dependent antigen. these mice have been tested for their ability to respond to three bacteriophages, t4, t7 and phix, sheep and horse erythrocytes, and alloantigens of c3h and c57bl/6 mice. the number of mice able to respond to each of these antigens was of the same order of magnitude. sheep and horse erythrocytes showed cross-reactivity at the t cell ... | 1978 | 76566 |
| contagious equine metritis 1977. | | 1978 | 76943 |
| agglutinins to the causative organism of contagious equine metritis 1977 in human serum. | | 1978 | 76949 |
| [strongyloides westeri ihle, 1917 (nematoda: strongyloididae. ii. parasitological and haematological features of experimental infection (author's transl)]. | experimental infections using 600,000 infective larvae of strongyloides westeri were carried out in seven worm-free shetland ponies, four foals and thee yearlings. in the foals, the prepatent period varied from ten to fourteen days, the patent period ranging from forty-three to eighty-three days. within approximately two months after infection, a decrease in the concentration of haemoglobin and an increase in the beta-globulin fraction of the protein pattern of the serum, practically coinciding ... | 1978 | 77053 |
| [effectiveness of coumaphos against chorioptes bovis in a horse with foot-mange (author's transl)]. | a horse with foot-mange failed to recover following a number of courses of treatment with coumaphos. mites isolated from this horse were tested in vitro for their sensitivity to coumaphos and lindane. the mites were resistant to coumaphos and sensitive to lindane. following treatment with lindane, the horse recovered within four weeks. | 1978 | 77054 |
| primary immune response of guinea pig spleen cells in vitro. | spleen cells of the primed guinea pig, when cultured alone, failed to make a primary immune response against heterologous erythrocytes. when supplemented with lymphocytes obtained from mineral oil induced peritoneal exudate (pel), a primary immune response against sheep, horse and chicken rbc was obtained. the kinetics, dose response and requirement of pels for this response are reported. | 1978 | 77295 |
| epidemiological features of the mycotoxicoses. | although mycotoxicoses attract more and more attention, the epidemiology of these diseases is still not adequately studied. the author devotes his study to certain diseases. in addition to the diseases whose mycotoxic nature is in no doubt, he conditionally includes certain diseases whose mycotoxic causation needs further proof. the author has assigned stachybotryotoxicosis and alimentary toxic aleukia or fusariotoxicosis to the first category and endemic nephropathy and primary liver-cell carci ... | 1977 | 77650 |
| isolation of human tumour-specific antigens associated with beta2 microglobulin. | in the present study the tube lai assay was used to monitor the isolation of the tsa of 4 different types of human cancers. each tumour antigen was found to be specific for tumours arising in the organ from which the tsa was initially derived and which were histopathologically similar. immunochemical studies revealed that these molecules co-isolate with normal human hla antigens and are associated with beta2m. on sephadex g-150, the majority of the papain-solubilized tumour antigen eluted in the ... | 1978 | 77673 |
| serum antiproteases and respiratory diseases of the horse. | the serum antiprotease (aat) levels are reported in healthy horses and horses with respiratory diseases. of the methods used, only the stic test seemed to give useful results; this test showed variations in horses with respiratory diseases, especially in horses with acute alveolar pulmonary emphysema. | 1977 | 77829 |