| chronic exposure to the parasite enteromyxum leei (myxozoa: myxosporea) modulates the immune response and the expression of growth, redox and immune relevant genes in gilthead sea bream, sparus aurata l. | the myxosporean parasite enteromyxum leei invades the intestine of gilthead sea bream producing a slow-progressing disease, which may end in the death of fish. the present work aimed to better know the host immune response and the underlying molecular mechanisms, which may help to understand why some individuals seem to be refractory to the disease. three main aspects involved in fish health and welfare (immune, growth and redox status) were studied in fish exposed to e. leei-contaminated efflue ... | 2008 | 18343157 |
| [aquaporins in gametogenesis of vertebrate animals]. | a review of the data on the presence, localization, and supposed role of aquaporin water channels in oocytes of xenopus laevis, oogenesis and maturation of teleosts sparus auratus and oncorhynchus mykiss, oogenesis and oocyte maturation of rats and mice, and spermatogenesis of several mammalians. | 2008 | 18409379 |
| monospecies and multispecies probiotic formulations produce different systemic and local immunostimulatory effects in the gilthead seabream (sparus aurata l.). | the effects of the oral administration of heat-inactivated lactobacillus delbrüeckii ssp. lactis and bacillus subtilis, individually or combined, on gilthead seabream immune responses were investigated both systemically and locally in the gut. in a first experiment, seabream (65 g) were fed for 3 weeks different diets supplemented with 1 x 10(7)cfu g(-1)lactobacillus, 1 x 10(7)cfu g(-1)bacillus, or 0.5 x 10(7)cfu g(-1)lactobacillus plus 0.5 x 10(7)cfu g(-1)bacillus. controls were fed non-supplem ... | 2008 | 18442923 |
| ultrastructure of enteromyxum leei (diamant, lom, & dyková, 1994) (myxozoa), an enteric parasite infecting gilthead sea bream (sparus aurata) and sharpsnout sea bream (diplodus puntazzo). | the ultrastructure of the developmental stages of the myxozoan enteromyxum leei parasitizing gilthead seabream (sparus aurata) intestine and sharpsnout sea bream (diplodus puntazzo) intestine and gallbladder are described. the earliest stage observed was a small dense trophozoite located among enterocytes. proliferative stages, observed intercellularly in the epithelium of the intestine and gallbladder as well as in the lumen, possessed the typical cell-in-cell configuration throughout their dev ... | 2008 | 18460155 |
| effects on growth and biochemical responses in juvenile gilthead seabream 'sparus aurata' after long-term dietary exposure to low levels of dioxins. | the farmed fish gilthead seabream (sparus aurata) were fed with a dry feed spiked with a low level (23 ng who-teq/kg of feed) polychlorinated dibenzo-p-dioxins and dibenzofurans (pcdd/fs) mixture in order to assess bioaccumulation of these contaminants in the muscle and liver tissues after long-term exposure (approximately 390 days). furthermore, effects on fish growth, feeding and on the response of some biochemical markers (induction of the cyp1a dependent erod activity, the conjugating enzyme ... | 2008 | 18472132 |
| molecular characterization of interleukin-6 in the gilthead seabream (sparus aurata). | a cdna clone, designated sbil-6 (seabream interleukin-6), was obtained from a cdna library of enriched immune-stimulated sequences from gilthead seabream. the deduced sbil-6 protein corresponds to a 225-amino acid protein with a putative 24-amino acid signal peptide, four conserved alpha helices and one n-linked glycosylation site. at the amino acid level sbil-6 shares 23-26% identity with mammalian il-6 sequences and 30-51% identity with other fish il-6 sequences. the structure of the sbil-6 ge ... | 2008 | 18513800 |
| the expression profile of tlr9 mrna and cpg odns immunostimulatory actions in the teleost gilthead seabream points to a major role of lymphocytes. | the potential effects of synthetic unmethylated oligodeoxynucleotides (odn) containing cpg motifs, mimicking bacterial dna, has never been evaluated on the immune response in the teleost fish gilthead seabream (sparus aurata), the most important fish species in mediterranean aquaculture. first, binding and competition studies have demonstrated that binding is saturated and promiscuous, suggesting the participation of several receptors. moreover, leucocyte cytotoxic (ncc) activity, production of ... | 2008 | 18535781 |
| a novel alternatively spliced transcript of cytosolic alanine aminotransferase gene associated with enhanced gluconeogenesis in liver of sparus aurata. | increased alanine aminotransferase (alt) activity is associated with insulin resistance and the development of type 2 diabetes. the aim of this study was to characterize the modulation of cytosolic alt expression in liver of gilthead sea bream (sparus aurata) under conditions associated with increased gluconeogenesis and in streptozotocin (stz)-treated fish. rt- and race-pcr assays allowed us to isolate a novel alt isozyme (calt2) generated from alternative splicing of calt gene in s. aurata. he ... | 2008 | 18582594 |
| histopathological changes caused by enteromyxum leei infection in farmed sea bream sparus aurata. | histological examinations were carried out on the stomach, pyloric caeca and 4 different parts of the intestine, as well as the rectum, hepatopancreas, gall bladder and spleen of 52 sea bream sparus aurata spontaneously infected by enteromyxum leei. fifteen fish from a non-infected farm were included as a control. clinical signs appeared only in extensively and severely infected fish. we observed enteromyxum leei almost exclusively in the intestinal tract, and very rarely in the intrahepatic bil ... | 2008 | 18589998 |
| cardicola aurata sp. n. (digenea: sanguinicolidae) from mediterranean sparus aurata l. (teleostei: sparidae) and its unexpected phylogenetic relationship with paradeontacylix mcintosh, 1934. | a new sanguinicolid trematode, cardicola aurata sp. n., is described from gilthead seabream sparus aurata l., from off the spanish mediterranean coast. the morphology of c. aurata sp. n. generally agrees with the diagnosis of the genus, however, in contrast to all other reported cardicola spp. the male pore is located sub-medially at the posterior end of the body instead of sinistrally before the posterior end of the body. based on a comparison of the morphology as well as partial 28s and its2 r ... | 2008 | 18639649 |
| nk-like and oxidative burst activities are the main early cellular innate immune responses activated after virus inoculation in reservoir fish. | viral diseases are a major problem in fish farming and a deeper knowledge of the immunological mechanisms playing a part in the antiviral defence is still important. moreover, fish farming practices (high densities, new areas of culture and egg/larvae/adult transport) are significantly increasing the spread of viruses and the number of susceptible or reservoir fish species. in this last point, no studies have focused on the immunological mechanisms playing a part in the antiviral responses in re ... | 2008 | 18657621 |
| effects of the organochlorines p,p'-dde and lindane on gilthead seabream leucocyte immune parameters and gene expression. | toxicological effects of pesticides in water-living animals are very important, especially when these animals are for human consumption. thus, in vitro tests were performed to evaluate the immunotoxicological impact of two organochlorines, lindane and p,p'-dde, on gilthead seabream (sparus aurata l.) leucocytes, an economically important fish-farmed species in the mediterranean area. leucocyte viability (apoptosis and necrosis), innate immune parameters (phagocytosis, respiratory burst and cell- ... | 2008 | 18757214 |
| seasonal trends and tissue distribution of organochlorine pollutants in wild and farmed gilthead sea bream (sparus aurata) from the western mediterranean sea and their relationship with environmental and biological factors. | seasonal trends and tissue distribution of organochlorine pesticides (ocps) and polychlorinated biphenyls (pcbs) in gilthead sea bream (sparus aurata) samples from the western mediterranean were investigated. hexane extracts of white and red muscle, liver, and gills from wild and cultured specimens were purified by normal-phase high-performance liquid chromatography, and selected ocps and pcbs were determined by gas chromatography coupled to mass spectrometry as indicators of contamination. the ... | 2009 | 18779940 |
| polymerase chain reaction amplification of repetitive intergenic consensus and repetitive extragenic palindromic sequences for molecular typing of pseudomonas anguilliseptica and aeromonas salmonicida. | the aim of this study was to evaluate the use of two molecular techniques, repetitive extragenic palindromic polymerase chain reaction (rep-pcr) and repetitive intergenic consensus pcr (eric-pcr), as epidemiological tools with which to discriminate among genetically distinct strains within two bacterial fish pathogens, pseudomonas anguilliseptica and aeromonas salmonicida. a total of 30 a. salmonicida and 52 p. anguilliseptica were analyzed. for p. anguilliseptica, three different major fingerpr ... | 2008 | 18783128 |
| application of in situ detection techniques to determine the systemic condition of lymphocystis disease virus infection in cultured gilt-head seabream, sparus aurata l. | immunohistochemistry (ihc) and in situ hybridization (ish) techniques have been used for the detection of lymphocystis disease virus (lcdv) in formalin-fixed, paraffin-embedded tissues from gilt-head seabream, sparus aurata l. diseased and recovered fish from the same population were analysed. ihc was performed with a polyclonal antibody against a 60-kda viral protein. a specific digoxigenin-labelled probe, obtained by pcr amplification of a 270-bp fragment of the gene coding the lcdv major caps ... | 2009 | 18803582 |
| structural and functional divergence of two fish aquaporin-1 water channels following teleost-specific gene duplication. | teleost radiation in the oceans required specific physiological adaptations in eggs and early embryos to survive in the hyper-osmotic seawater. investigating the evolution of aquaporins (aqps) in these vertebrates should help to elucidate how mechanisms for water homeostasis evolved. the marine teleost gilthead sea bream (sparus aurata) has a mammalian aquaporin-1 (aqp1)-related channel, termed aqp1o, with a specialized physiological role in mediating egg hydration. however, teleosts have an add ... | 2008 | 18811940 |
| effect of in vitro exposure to vibrio vulnificus on hydroelectrolytic transport and structural changes of sea bream (sparus aurata l.) intestine. | the everted gut sac technique has been used to investigate the effect of vibrio vulnificus on water and electrolyte (na(+), k(+), cl(-), hco(3)(-)) transport on the intestine of sea bream (sparus aurata l.). both the anterior and the posterior intestine were incubated in a medium containing 10(8) v. vulnificus cells ml(-1) at 25 degrees c for 2 h. the presence of v. vulnificus resulted in a significant reduction (p < 0.05) of water absorption in the anterior intestine, while sodium absorption in ... | 2009 | 18825505 |
| viral encephalopathy and retinopathy of dicentrarchus labrax and sparus aurata farmed in tunisia. | viruses belonging to the nodaviridae family cause disease worldwide among a large number of species of marine fish, and have been described in all continents. in the present study, a total of 69 farmed tunisian sea bass (dicentrarchus labrax) and 24 sea bream (sparus aurata) samples were tested monthly for the detection of betanodavirus. the virus was identified in both species using indirect immunofluorescence assays (ifat) and rt-pcr. in addition sequence analysis of part of the coat protein g ... | 2009 | 18982421 |
| oral delivery of live yeast debaryomyces hansenii modulates the main innate immune parameters and the expression of immune-relevant genes in the gilthead seabream (sparus aurata l.). | microorganisms isolated from fish can be used as prophylactic tools for aquaculture in the form of probiotic preparations. the purpose of this study was to evaluate the effects of dietary administration of the live yeast debaryomyces hansenii cbs 8339 on the gilthead seabream (sparus aurata l.) innate immune responses. seabream were fed control or d. hansenii-supplemented diets (10(6) colony forming units, cfu g(-1)) for 4 weeks. humoral (seric alternative complement and peroxidase activities), ... | 2008 | 19004644 |
| some organochlorine pesticide residues in fish species in konya, turkey. | the levels of organochlorine pesticides were measured in 18 fish species from konya markets, turkey. these species were selected on the basis of their importance to local human fish consumption. the extracted residues were analyzed on a micro capillary gas chromatograph equipped with an electron capture detector. total 14 different organochlorine pesticides were determined. these residues were detected in all fish species, except in trout, horse mackerel and bonito. ddt and its metabolites and h ... | 2009 | 19103455 |
| adhesive properties of environmental vibrio alginolyticus strains to biotic and abiotic surfaces. | the ability of vibrio alginolyticus strains isolated from a bathing and fishing area (khenis, centre of tunisia) to adhere to both biotic and abiotic surfaces was evaluated in the present work. the biochemical, physiological and enzymatic activities of all strains was also investigated. three morphotypes of v. alginolyticus were obtained on congo red agar and only 14 strains produced black colonies. the majority of strains were able to degrade the skin mucus of both sparus aurata and dicentrarch ... | 2008 | 19123304 |
| biochemical response in gilthead seabream (sparus aurata) to in vivo exposure to a mix of selected pahs. | in our previous studies we assessed the biochemical response of juvenile seabream to the exposure to three selected polycyclic aromatic hydrocarbons (pahs) (phenanthrene, pyrene and fluorene) in short-term laboratory experiments. since in the analyses conducted in the field it is much more natural to expect the presence of multiple contaminants at the same time, the objective of this study was to analyse the reaction of seabream to the exposure to a mix of the three pahs tested previously. the p ... | 2009 | 19167757 |
| high potential of adhesion to abiotic and biotic materials in fish aquaculture facility by vibrio alginolyticus strains. | the ability of vibrio alginolyticus strains isolated from sparus aurata and dicentrarchus labrax nursery to adhere to epithelial cell lines (hep-2 and caco-2), fish mucus and their ability to form a biofilm on different surfaces (glass, polystyrene, polyethylene and polyvinyl-chloride) was investigated in this study. | 2009 | 19245411 |
| a yeast assay based on the gilthead sea bream (teleost fish) estrogen receptor beta for monitoring estrogen mimics. | a yeast (saccharomyces cerevisiae)-based assay was developed and tested with steroids and chemicals (mostly pesticides). the induction of beta-galactosidase activity was strictly dependent on the presence of seabream (sparus aurata) betaa estrogen receptor (sberbetaa) and substances known to have estrogenic activity. 17beta-estradiol (e(2)) and diethylstilbestrol (des), both agonists, were most active and the antagonist tamoxifen (tam) was 14-fold less active than e(2). among the chemicals teste ... | 2009 | 19303142 |
| description of enterovibrio nigricans sp. nov., reclassification of vibrio calviensis as enterovibrio calviensis comb. nov. and emended description of the genus enterovibrio thompson et al. 2002. | eleven strains of halophilic, facultative anaerobes isolated from healthy and diseased dentex dentex and sparus aurata (bony fishes) cultured in spanish mediterranean fisheries have been studied by a polyphasic approach that included a wide phenotypic characterization, dna-dna hybridization and phylogenetic analysis using 16s rrna, reca and rpod gene sequences. all strains were phylogenetically related to enterovibrio species and vibrio calviensis. on the basis of sequence analysis and dna-dna h ... | 2009 | 19329591 |
| isolation and characterization of toll-like receptor 9 in half-smooth tongue sole cynoglossus semilaevis. | toll-like receptors (tlrs) are considered as key sensors to trigger the host's innate immune system and adaptive immune responses by recognizing various pamps and initiating signal transduction. tlr9, as a member of tlr family, mediates the recognition of unmethylated cpg dinucleotide motifs commonly found in both bacterial and viral genomes. in the current study, the tlr9 gene was isolated from one of flatfish species, half-smooth tongue sole (cynoglossus semilaevis). in the 4588 bp genomic seq ... | 2009 | 19340952 |
| profiling of infection specific mrna transcripts of the european seabass dicentrarchus labrax. | the european seabass (dicentrarchus labrax), one of the most extensively cultured species in european aquaculture productions, is, along with the gilthead sea bream (sparus aurata), a prospective model species for the perciformes which includes several other commercially important species. massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. revealing transcripts involved in immune response and studying their relative expression enhances the und ... | 2009 | 19361338 |
| comparison of biochemical composition of three aqua cultured fishes (dicentrarchus labrax, sparus aurata, dentex dentex). | the moisture, ash, protein and fat content and amino acid, fatty acid and mineral compositions in the flesh of three commercially important fish species (dicentrarchus labrax, sparus aurata, dentex dentex) from the aqua culture of turkey were evaluated. ranges of moisture, ash, protein and fat contents were 69.68-76.42%, 1.49-1.95%, 18.21-21.70% and 2.29-8.10%, respectively. among them, those occurring in the highest proportions were aspartic acid (3238.91-5204.71 mg/100 g), glutamic acid (5256. ... | 2008 | 19382346 |
| gyrodactylus orecchiae sp. n. (monogenea: gyrodactylidae) from farmed populations of gilthead seabream (sparus aurata) in the adriatic sea. | gyrodactylus orecchiae sp. n. (monogenea, gyrodactylidae) is described from the skin, fins, eyes and gills of juvenile sparus aurata l. (gilthead seabream) following two outbreaks of gyrodactylosis amongst stocks held in inshore floating cages on the adriatic coast of albania and croatia. fish were heavily infected (1000+ gyrodactylids/fish) with g. orecchiae which reportedly resulted in approximately 2-10% mortality amongst the infected stock. morphologically, the haptoral hooks of g. orecchiae ... | 2009 | 19391328 |
| evaluation of candidate reference genes for qpcr during ontogenesis and of immune-relevant tissues of european seabass (dicentrarchus labrax). | the expression level of mrna can vary significantly in different experimental conditions, such as stress, infection, developmental stage or tissue. suitable reference genes are expected to exhibit constant expression levels. however no single gene is constitutively expressed in all cell types and under all experimental conditions. it has become clear that expression stability of the intended reference gene has to be examined before each experiment. for expression studies using quantitative real- ... | 2009 | 19398033 |
| heavy metal bioaccumulation and metallothionein content in tissues of the sea bream sparus aurata from three different fish farming systems. | the distribution and potential bioaccumulation of dietary and waterborne cadmium and lead in tissues of sea bream (sparus aurata), a major aquaculture species, was studied in relation to three different fish farming systems. metallothionein levels in fish tissues were also evaluated. results demonstrate that metal concentrations in various tissues significantly vary among fish culture systems. different tissues show different capacity for accumulating heavy metals. the content of both cadmium an ... | 2010 | 19444632 |
| stimulation of gut immune system by early administration of probiotic strains in dicentrarchus labrax and sparus aurata. | early feeding (started during gut metamorphosis and establishment of the hypothalamic-pituitary-interrenal axis) with probiotic-supplemented diets, besides modifying the intestinal microflora, evoked profound effects on the physiology of fish larvae. using rotifers and artemia as living vectors, the autochthonous bacterium lactobacillus delbrueckii delbrueckii or a multispecies probiotic formulation (autochthonous lactobacillus fructivorans+lactobacillus plantarum from human feces) were orally a ... | 2009 | 19456355 |
| invasion and survival of photobacterium damselae subsp. piscicida in non-phagocytic cells of gilthead sea bream, sparus aurata l. | fluorescence microscopy and gentamicin protection assays were used to investigate the ability of four photobacterium damselae subsp. pisicida (phdp) strains to adhere to and to invade the fish epithelial cell line, saf-1, derived from sparus aurata. all strains tested were detected intracellularly using both techniques, although internalization levels varied among strains. treatment with cytochalasin d and experiments carried out at 4 degrees c demonstrated that a functional host cell cytoskelet ... | 2009 | 19460085 |
| coordination of deiodinase and thyroid hormone receptor expression during the larval to juvenile transition in sea bream (sparus aurata, linnaeus). | to test the hypothesis that ths play an important role in the larval to juvenile transition in the marine teleost model, sea bream (sparus auratus), key elements of the thyroid axis were analysed during development. specific rt-pcr and taqman quantitative rt-pcr were established and used to measure sea bream iodothyronine deiodinases and thyroid hormone receptor (tr) genes, respectively. expression of deiodinases genes (d1 and d2) which encode enzymes producing t3, trs and t4 levels start to inc ... | 2010 | 19549532 |
| evidence for the involvement of aquaporins in sperm motility activation of the teleost gilthead sea bream (sparus aurata). | the expression of aquaporins in the spermatozoa of the marine teleost gilthead sea bream (sparus aurata) and their involvement in the motility activation process were investigated. sperm motility was activated by a hyperosmotic shock, but it was completely inhibited by 10 microm hgcl(2), such inhibition being partially recovered by beta-mercaptoethanol (me). conventional rt-pcr using primers specific for s. aurata aquaglyceroporin (glp) and aquaporin 1a (aqp1a) demonstrated the presence of both ... | 2009 | 19571262 |
| experimental transmission of sparicotyle chrysophrii (monogenea: polyopisthocotylea) to gilthead seabream (sparus aurata) and histopathology of the infection. | the polyopisthocotylean sparicotyle chrysophrii (van beneden et hesse, 1863) was experimentally transmitted to gilthead seabream (sparus aurata l.) by exposure to eggs (egt) and by cohabitation with naturally parasitized fish (ct). in egt trials, the infection was successfully transmitted by introducing containers with monogenean eggs in the fish tanks, with the highest infection level (85.7% prevalence, 3.3 mean intensity) achieved at 6 weeks post exposure (p.e.) to the infection dose of 650 eg ... | 2009 | 19606789 |
| evaluation of the possible transmission of bse and scrapie to gilthead sea bream (sparus aurata). | in transmissible spongiform encephalopathies (tses), a group of fatal neurodegenerative disorders affecting many species, the key event in disease pathogenesis is the accumulation of an abnormal conformational isoform (prp(sc)) of the host-encoded cellular prion protein (prp(c)). while the precise mechanism of the prp(c) to prp(sc) conversion is not understood, it is clear that host prp(c) expression is a prerequisite for effective infectious prion propagation. although there have been many stud ... | 2009 | 19636413 |
| metals in tissues of fish from yelkoma lagoon, northeastern mediterranean. | this study was performed to investigate the metal concentrations in muscle, liver, gonad, and gill of gilthead seabream (sparus aurata l., 1758), european seabass (dicentrarchus labrax l., 1758), and keeled mullet (liza carinata valenciennes, 1836) from yelkoma lagoon, northeastern mediterranean region. so, the levels of cadmium, cobalt, chromium, copper, iron, manganese, nickel, lead, and zinc in tissues of specimens from the lagoon were determined by inductively coupled plasma optical emission ... | 2010 | 19680760 |
| anandamide and am251, via water, modulate food intake at central and peripheral level in fish. | the endocannabinoid system is a major regulator of food intake in many animal species. studies conducted so far have mostly focused on mammals, and, therefore, in this study, the role of the endocannabinoid system in food intake in the sea bream sparus aurata was investigated. the effect of different doses of the endocannabinoid anandamide (aea), administered via water, was evaluated after different exposure times (30, 60 and 120 min) at both physiological and molecular levels. the results obtai ... | 2010 | 19800340 |
| investigation of several virulence properties among vibrio alginolyticus strains isolated from diseased cultured fish in tunisia. | we analysed 34 vibrio alginolyticus strains isolated from gilthead sea bream sparus aurata l. and sea bass dicentrarchus labrax l. cultured in fish farms on the tunisian mediterranean coast for the presence of several virulence properties such as extracellular products (ecp) production, growth in iron-limiting conditions and survival in fish serum. the results obtained with different substrates showed that ecp of v. alginolyticus were hydrolytic. the virulence was correlated with the ability of ... | 2009 | 19899346 |
| multiple cu-atpase genes are differentially expressed and transcriptionally regulated by cu exposure in sea bream, sparus aurata. | copper (cu) is an essential metal, although in excess is highly toxic due to its redox properties and, therefore intracellular cu homeostasis is a highly regulated process. cu-atpases are pivotal regulatory, proteins of intracellular and bodily cu homeostasis. two cu-atpases, atp7a and atp7b with distinct, functions are found in mammals and herein we report the structure and expression under cu stress of, homologues of atp7a and atp7b in gilthead sea bream (sparus aurata), the first such report ... | 2010 | 20044148 |
| cloning of wap65 in sea bass (dicentrarchus labrax) and sea bream (sparus aurata) and expression in sea bass tissues. | the complementary dna encoding wap65 protein was cloned from the liver of two fish species sea bass (dicentrarchus labrax) and sea bream (sparus aurata). full-length cdna sequences were obtained from reverse transcribed total rna, followed by 5' and 3' rapid amplification of cdna end (race) experiments. the full-length cdna sequence of d. labrax is 1709bp and the coding sequence is flanked by a 67bp 5'-utr and a 358bp 3'-utr. the full-length cdna sequence of s. aurata is 1599bp, and the coding s ... | 2010 | 20085823 |
| lectinhistochemical detection of terminal carbohydrate residues in the enteric myxozoan enteromyxum leei parasitizing gilthead seabream sparus aurata (pisces: teleostei): a study using light and transmission electron microscopy. | the presence of terminal carbohydrate residues in enteromyxum leei (diamant, lom et dyková, 1994) palenzuela, redondo et alvarez-pellitero, 2002 stages in gilthead seabream intestines was studied at light microscopy (lm) and transmission electron microscopy (tem) level using lectin histochemical techniques. abundant mannose and/or glucose residues were demonstrated by the intense staining caused by binding of biotinylated concanavalin a (con a), at both lm and tem. a clear positivity was also ob ... | 2009 | 20128238 |
| identification of vibrio harveyi as a causative bacterium for a tail rot disease of sea bream sparus aurata from research hatchery in malta. | a bacterial disease was reported from gilthead sea bream (sparus aurata) within a hatchery environment in malta. symptoms included complete erosion of tail, infection in the eye, mucous secretion and frequent mortality. a total of 540 strains were initially isolated in marine agar from different infected body parts and culture water sources. subsequently 100 isolates were randomly selected, identified biochemically and all were found to be vibrio harveyi-related organisms; finally from 100 isola ... | 2010 | 20129765 |
| crtac1 homolog proteins are conserved from cyanobacteria to man and secreted by the teleost fish pituitary gland. | cartilage acidic protein 1 (crtac1) gene expression is used as a marker for chondrocyte differentiation in stem cell-based tissue engineering. it is also transcribed outside the skeleton where at least two different transcripts are expressed in lung and brain. in the pituitary gland of the teleost fish sea bream sparus auratus, we have found a transcript with a high degree of sequence identity to crtac1 family members but lacking the egf-like calcium-binding domain encoding sequence of crtac1 an ... | 2010 | 20171266 |
| molecular cloning and characterization of adult sparus aurata hemoglobin genes. | among teleosts, sparus aurata occupies a prominent place in the gastronomic and economic fields of the mediterranean basin and other geographic districts. the knowledge of its molecular structures and functional features, such as hemoglobin, may be helpful to understand the adaptive biochemical mechanisms that allow this fish to live under extreme conditions, including fish farming. in sparus aurata red blood cells two different alpha and one beta hemoglobin genes have been identified. the alpha ... | 2010 | 20210659 |
| the essential and toxic elements in tissues of six commercial demersal fish from eastern mediterranean sea. | the essential elements and contaminants (pb and cd) were determined seasonally in the muscle and liver of six demersal fish species sparus aurata, chelidonichthys lucernus, upeneus molluccensis, solea solea, merluccius merluccius and saurida undosquamis from the iskenderun bay, eastern mediterranean sea. the concentrations in muscles of fish species were 474-1534 mg/kg for na, 574-3374 mg/kg for k, 72.7-496 m/kg ca, 94.1-210 mg/kg for mg. muscle accumulated the lowest levels of elements. trace e ... | 2010 | 20214948 |
| sparicotyle chrysophrii (van beneden and hesse 1863) (monogenea: polyopisthocotylea) parasite of cultured gilthead sea bream sparus aurata (linnaeus 1758) (pisces: teleostei) from corsica: ecological and morphological study. | sparicotyle chrysophrii infections were highlighted during a parasitological monitoring carried out on cultured gilthead sea bream sparus aurata linnaeus 1758 from aquaculture sites in corsica. factors and biological mechanisms regulating parasites populations, particularly controlling the infestation and survival of monogeneans have been studied. variations in infection support the hypotheses that the main factor determining the infection of s. chrysophrii was temperature and that the parasite ... | 2010 | 20422218 |
| accumulation of some heavy metals in hysterothylacium aduncum (nematoda) and its host sea bream, sparus aurata (sparidae) from north-eastern mediterranean sea (iskenderun bay). | the sea bream's nematode and sparus aurata, sampled from the iskenderun bay, mediterranean, in march 2008 were analyzed by inductively coupled plasma-atomic emission spectrometry for their some heavy metal (cd, cr, cu, fe, hg, mn, mg, pb, and zn) levels. the metal concentrations of the parasites were compared to different organs (liver, muscle, gill, intestine, and skin) of the fish hosts. there were significant differences in cd, cr, cu, fe, mn, zn, hg, mg, and pb concentrations in tissues of f ... | 2011 | 20422284 |
| effect of ivermectin on the liver of gilthead sea bream sparus aurata: a proteomic approach. | gilthead sea bream sparus aurata is the most commercialized mediterranean aquacultured fish species. ivermectin has recently (experimentally) started to be used to control ectoparasitic infestations in mediterranean cultured marine fish. the potential hepatotoxicity of ivermectin was investigated in gilthead sea bream juveniles (35g) following oral administration at the recommended dose of 0.2 mgkg(-1) fish for 10d. difference gel electrophoresis technology (dige) was used to study the effect of ... | 2010 | 20451238 |
| identification of a novel transforming growth factor-beta (tgf-beta6) gene in fish: regulation in skeletal muscle by nutritional state. | the transforming growth factor-beta (tgf-beta) family constitutes of dimeric proteins that regulate the growth, differentiation and metabolism of many cell types, including that of skeletal muscle in mammals. the potential role of tgf-betas in fish muscle growth is not known. | 2010 | 20459854 |
| phylogenetic consistencies among chondrichthyan and teleost fishes in their bioaccumulation of multiple trace elements from seawater. | multi-tracer experiments determined the accumulation from seawater of selected radioactive trace elements (mn-54, co-60, zn-65, cs-134, am-241, cd-109, ag-110m, se-75 and cr-51) by three teleost and three chondrichthyan fish species to test the hypothesis that these phylogenetic groups have different bioaccumulation characteristics, based on previously established contrasts between the carcharhiniform chondrichthyan scyliorhinus canicula (dogfish) and the pleuronectiform teleost psetta maxima (t ... | 2010 | 20471062 |
| [assessment of seawater quality along the asinara gulf. note 1: preliminary results of seawater monitoring using dicentrarchus labrax and sparus aurata as bioindicators]. | in recent years, physical-chemical, chemical and microbiological testing systems to water's control was matched by the use of "biomarkers" such as algae, nematodes, anellidi, porifera, molluscs and arthropods (crustaceans), although these are phylogenetically distant from humans and they differ in methods of recruitment, toxico-kinetics and metabolism of xenobiotics. that is why today the predatory fishes (tuna, mackerel, sea bream, sea bass and swordfish) are among the most widely used in biomo ... | 2010 | 20476660 |
| a new genotype of lymphocystivirus isolated from cultured gilthead seabream, sparus aurata l., and senegalese sole, solea senegalensis (kaup). | | 2010 | 20487141 |
| correlated expression profile of extracellular matrix-related molecules during the inflammatory response of the teleost fish gilthead seabream. | extracellular matrix (ecm) components, in addition to their structural functions, interact with cell surface receptors and intracellular components to modulate the transduction of signals for cell growth, differentiation, migration, proliferation, polarization, apoptosis and inflammation. our previous findings in the gilthead seabream (sparus aurata l.), a marine seasonal hermaphrodite teleost fish, have shown that both endocrine and immune stimuli modulate the expression of matrix metalloprotea ... | 2010 | 20488200 |
| carbohydrate patterns in the digestive tract of sparus aurata l. and psetta maxima (l.) (teleostei) parasitized by enteromyxum leei and e. scophthalmi (myxozoa). | the influence of enteromyxum spp. infections on the carbohydrate patterns of the digestive tract of gilthead sea bream (gsb) sparus aurata l. and turbot (tb) psetta maxima (l.) has been studied. histochemical stainings to differentiate the types of mucins and lectin-binding assays to detect terminal carbohydrate residues were applied to histological sections of gsb and tb uninfected or infected by enteromyxum leei and e. scophthalmi, respectively. the number of intestinal gc decreased in severel ... | 2010 | 20601108 |
| absence of anisakid larvae in farmed european sea bass (dicentrarchus labrax l.) and gilthead sea bream (sparus aurata l.) in southeast spain. | in the present study, a total of 871 farmed fish, 612 gilthead sea bream (sparus aurata l.) and 259 european sea bass (dicentrarchux labrax l.), were examined for the presence of anisakid larvae. two diagnostic methods were applied, visual inspection and artificial digestion based on the degradation of fish soft tissue in an acidified pepsin enzyme solution. none of the samples examined in this study contained any anisakid parasite. the results suggest that consumption of these farmed fish speci ... | 2010 | 20615348 |
| replacement of dietary fish oil by vegetable oils affects humoral immunity and expression of pro-inflammatory cytokines genes in gilthead sea bream sparus aurata. | commercial gilthead sea bream feeds are highly energetic, fish oil traditionally being the main lipid source. but the decreased fish oil production together with the increased prices of this oil encourages its substitution by vegetable oils, imposing new nutritional habits to aquaculture species. partial replacement of fish oil by vegetable oils in diets for marine species allows good feed utilization and growth but may affect fish health, since imbalances in dietary fatty acids may alter fish i ... | 2010 | 20817101 |
| prolactin-induced production of reactive oxygen species and il-1β in leukocytes from the bony fish gilthead seabream involves jak/stat and nf-κb signaling pathways. | prolactin (prl), a peptide hormone produced by the pituitary gland, was shown to play an important role in the modulation of the immune system of lower and higher vertebrates. to further investigate the effects of prl on the activation of professional phagocytes of bony fish, we stimulated head kidney leukocytes and purified macrophages from the gilthead seabream (sparus aurata l.) with various physiological concentrations of native salmon prl for 2 and 16 h and analyzed the respiratory burst ac ... | 2010 | 20826756 |
| scarcity of parasite assemblages in the adriatic-reared european sea bass (dicentrarchus labrax) and sea bream (sparus aurata). | the shaping forces of parasite community structure still is the main subject in the ecological parasitology whilst community predictability and repeatability showed that hardly a generally applicable role is ever going to be assessed. defining and describing parasite communities can be very useful from the epizootiological point, in order to help in the assessment of the medical and economical impact of certain parasitosis, moreover when hosts are economically valuable species. since parasite as ... | 2010 | 20837380 |
| influence of moraxella sp. colonization on the kidney proteome of farmed gilthead sea breams (sparus aurata, l.). | abstract: | 2010 | 20939867 |
| quantitative trait loci for resistance to fish pasteurellosis in gilthead sea bream (sparus aurata). | fish pasteurellosis is a bacterial disease causing important losses in farmed fish, including gilthead sea bream, a teleost fish of great relevance in marine aquaculture. we report in this study a qtl analysis for resistance to fish pasteurellosis in this species. an experimental population of 500 offspring originating from eight sires and six dams in a single mass-spawning event was subjected to a disease challenge with photobacterium damselae subsp. piscicida (phdp), the causative agent of fis ... | 2010 | 20946317 |
| the nutritional background of the host alters the disease course in a fish-myxosporean system. | the aim of the present work was to determine if a practical plant protein-based diet containing vegetable oils (vo) as the major lipid source could alter the disease course when challenged with the myxosporean enteromyxum leei, a wide-spread parasite in the mediterranean basin causing heavy economic losses. gilthead sea bream (sparus aurata) fed for 9 months either a fish oil (fo) diet or a blend of vos at 66% of replacement (66vo diet) were challenged by exposure to parasite-contaminated water ... | 2010 | 20947256 |
| changes in complement responses in gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) under crowding stress, plus viral and bacterial challenges. | gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) were subjected to either experimental infection with photobacterium damselae subsp. piscicida or nodavirus after a period of 2 weeks of crowding in which fish were subjected to a 5-fold increase in density (10-50 kg/m(3)). samples were obtained before the crowding period (0 h or control) and at 24h and 72 h after crowding from both groups of infected fish. the complement haemolytic activity and the expression of the c3 ... | 2010 | 20951810 |
| novel horizontal transmission route for enteromyxum leei (myxozoa) by anal intubation of gilthead sea bream sparus aurata. | the aim of the present study was to determine whether enteromyxum leei, one of the most threatening parasitic diseases in mediterranean fish culture, could be transmitted by peranal intubation in gilthead sea bream sparus aurata l. fish were inoculated either orally or anally with intestinal scrapings of infected fish in 3 trials. oral transmission failed, but the parasite was efficiently and quickly transmitted peranally. prevalence of infection was 100% at 60 d post inoculation (p.i.) in trial ... | 2010 | 21166314 |
| microbial ecology of fish species ongrowing in greek sea farms and their watery environment. | the present study focuses on the bacteriological profile of both watery ecosystem and fishes from different north and central west greek fish-farms ongrowing euryhaline fish species. the natural microflora of the fish and the water of their ongrowing units in selected farms were studied for a period of 20 months. the analyzed samples were mainly sea bream (sparus aurata) 61.3% and sea bass (dicentrarchus labrax) 24%. in most of the watery ecosystems coming from the different sampling areas, tota ... | 2011 | 21421068 |
| differential effects on proliferation of gh and igfs in sea bream (sparus aurata) cultured myocytes. | primary culture of gilthead sea bream skeletal muscle cells was used to examine the effects of growth hormone (gh) and insulin-like growth factors (igfs) in fish muscle proliferation and growth. proliferation was measured as the percentage of positive cells expressing the proliferating cell nuclear antigen (pcna) analyzed by immunocytochemistry. first, the effects of gh from two different origins (mammals and fish) were tested. gh from human (hgh) did not stimulate proliferation except at 3h at ... | 2011 | 21458458 |
| molecular and functional characterization of the gilthead seabream β-defensin demonstrate its chemotactic and antimicrobial activity. | antimicrobial peptides (amps) are important mediators of the innate immune response against bacteria and viruses. we have found a β-defensin (bd) gene searching the expressed sequence tags (ests) of the teleost fish gilthead seabream (sparus aurata). the clone contains an open reading frame of 201bp mrna that encodes a putative seabream β-defensin (sabd) propeptide of 66 amino acids containing the six conserved cysteines as the main signature of this amp. the phylogenetic tree shows that sabd, a ... | 2011 | 21497909 |
| first report of streptococcus iniae in red porgy (pagrus pagrus, l.). | this work describes the first isolation of streptococcus iniae in red porgy, pagrus pagrus (l.), and the first european isolation of this pathogen in gilthead seabream, sparus aurata (l.). in both farmed fish species, infection resulted in lethargy, anorexia, abnormal swimming, exophthalmia and sudden death, with mortality rates of over 25% in red porgy and 10% in gilthead seabream. beta-haemolytic gram-positive cocci, catalase negative and oxidase negative, were isolated in pure culture from in ... | 2010 | 21504081 |
| gilthead seabream (sparus aurata) carrying antibiotic resistant enterococci. a potential bioindicator of marine contamination? | antibiotic resistance in bacteria is a growing problem that is not only restricted to the clinical setting but also to other environments such as marine species that harbor antibiotic resistant bacteria and therefore may serve as reservoirs for antibiotic-resistance genetic determinants. the aim of this study was to evaluate antibiotic resistance phenotypes in enterococci isolated from fecal samples of gilthead seabream and the associated mechanisms of resistance. a collection of 118 samples wer ... | 2011 | 21511306 |
| mx expression in gilthead sea bream (sparus aurata l.) in response to poly i:c, bacterial lps and chromosomal dna: preliminary study. | | 2011 | 21575726 |
| molecular characterization and expression analysis of six peroxiredoxin paralogous genes in gilthead sea bream (sparus aurata): insights from fish exposed to dietary, pathogen and confinement stressors. | the aim of this work was to underline the physiological role of the antioxidant peroxiredoxin (prdx) family in gilthead sea bream (sparus aurata l.), a perciform fish extensively cultured in the mediterranean area. first, extensive blast searches were done on the gilthead sea bream cdna database of the aquamax european project (www.sigenae.org/iats), and six contigs were unequivocally identified as prdx1-6 after sequence completion by rt-pcr. the phylogenetic analysis evidenced three major clade ... | 2011 | 21640832 |
| gilthead seabream (sparus aurata) as carriers of shv-12 and tem-52 extended-spectrum beta-lactamases-containing escherichia coli isolates. | abstract in recent years, bacterial resistance to beta-lactam antibiotics has risen dramatically in escherichia coli isolated from animals that could pass through the food chain to humans. one hundred eighteen fecal samples of sparus aurata were tested for extended-spectrum beta-lactamase (esbl)-containing e. coli recovery. susceptibility to 16 antimicrobial agents was performed by disk diffusion. esbl-phenotypic detection was carried out by double-disk test, and the presence of genes encoding t ... | 2011 | 21657938 |
| analysis of the genetic diversity of the lymphocystis virus and its evolutionary relationship with its hosts. | lymphocystis disease virus (lcdv) is the causative agent of lymphocystis disease. in this study, the mcp gene of lcdv and the cyt b gene of the host fish were selected as molecular markers, and the phylogenetic relationships between lcdv and its host were analyzed. the 25 lcdv isolates examined in this study were attributed to seven lcdv genotypes: genotype i (lcdv-1), genotype ii (lcdv-cn, etc.), genotype iii (lcdv-rf), genotype iv (lcdv-rc and lcdv-sb), genotype v (lcdv-cb), genotype vi (lcdv- ... | 2011 | 21805162 |
| molecular profiling of the gilthead sea bream (sparus aurata l.) response to chronic exposure to the myxosporean parasite enteromyxum leei. | the aim of the present work was to investigate the transcriptome response of gilthead sea bream (sparus aurata) after challenge with the myxosporean enteromyxum leei, a wide-spread enteric parasite causing heavy economic losses in mediterranean sparid farms. this parasite causes severe desquamative enteritis which usually leads to death of the fish, and there are no preventative or curative treatments for this enteromyxosis. after 113 days of exposure to parasite-contaminated effluent, fish were ... | 2011 | 21803425 |
| immune response of gilthead seabream (sparus aurata) following experimental infection with aeromonas hydrophila. | the gram-negative bacteria aeromonas hydrophila is a heterogeneous organism that causes the disease known as motile aeromonad septicaemia, which is responsible for serious economic loss in seabream culture due to bacterial infections. however, the immune mechanisms involved in this disease in fish are still poorly understood. for the purpose of this study, gilthead seabream (sparus aurata l.) specimens received a double intraperitoneal injection of bacterial inoculums: a primary infection with 1 ... | 2011 | 21767651 |
| infiltration and activation of acidophilic granulocytes in skin lesions of gilthead seabream, sparus aurata, naturally infected with lymphocystis disease virus. | light, ultrastructural and immunocytochemical investigations were carried out on the skin of gilthead seabream, sparus aurata l., naturally infected with lymphocystis iridovirus, to assess pathology and host cellular responses. of 220,000 young seabream examined, 32,400 (14.7%) had clinical signs of lymphocystis and within 6months of disease appearance, 45% of clinically affected fish had died. a subsample of 20 s. aurata (80.0±12.5mm total length, mean±s.d.), including 10 with lymphocystis on t ... | 2011 | 21762724 |
| influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (sparus aurata) and intestinal contents in goldfish (carassius auratus). | fish intestinal microbiota changes with the diet and this effect is of particular interest considering the increasing substitution of fish meal by plant protein sources. the objective of this work was to study the effects of partial substitution of fish meal with lupin and rapeseed meals on gut microbiota of the gilthead sea bream (sparus aurata) and in goldfish (carassius auratus). faecal, gastrointestinal and intestinal contents were characterized using culture-based and molecular methods. vib ... | 2011 | 21692817 |
| gyrodactylus longipes n. sp. (monogenea: gyrodactylidae) from farmed gilthead seabream (sparus aurata l.) from the mediterranean. | gyrodactylus longipes n. sp. (monogenea, gyrodactylidae) is described from the gills of farmed juvenile gilthead seabream (sparus aurata l.) from two sites located in italy and bosnia-herzegovina and represents the second species of gyrodactylus to be described from s. aurata. gyrodactylus orecchiae paladini, cable, fioravanti, faria, di cave et shinn, 2009 was the first gyrodactylid to be described from s. aurata, from populations cultured in albania and croatia. in the current study, g. longip ... | 2011 | 21723959 |
| gilthead sea bream (sparus auratus) and european sea bass (dicentrarchus labrax) expressed sequence tags: characterization, tissue-specific expression and gene markers. | the gilthead sea bream, sparus auratus, and the european sea bass, dicentrarchus labrax, are two of the most important marine species cultivated in southern europe. this study aimed at increasing genomic resources for the two species and produced and annotated two sets of 30,000 expressed sequence tags (est) each from 14 normalized tissue-specific cdna libraries from sea bream and sea bass. clustering and assembly of the ests formed 5268 contigs and 12,928 singletons for sea bream and 4573 conti ... | 2010 | 21798212 |
| accumulation of ivermectin in the brain of sea bream, sparus aurata after intraperitoneal administration. | ivermectin, which is widely used in veterinary and human, has been considered safe due to its inability to penetrate into the central nervous system of higher vertebrates. this paper presents data on the ability of the drug to cross the blood-brain barrier of the marine teleost sea bream, sparus aurata and accumulate in the brain. the concentration of the drug in the brain and the serum of the fish was assessed by the use of a direct competitive elisa commercial kit. our results showed a rapid u ... | 2004 | 21782707 |
| Differential antiviral activity of Mx1, Mx2 and Mx3 proteins from gilthead seabream (Sparus aurata) against Infectious Pancreatic Necrosis Virus (IPNV). | Mx proteins are crucial effectors of the innate antiviral response mediated by the interferon type I signalling pathway. Recently, three Mx proteins, named SauMx1, SauMx2 and SauMx3, corresponding to three different genes, have been identified in the cultured marine species gilthead seabream (Sparus aurata). In this study, the three SauMx cDNAs were cloned into expression vectors and used for transfection of CHSE-214 cells. Monoclonal cell populations stably expressing each recombinant protein h ... | 2011 | 21872932 |
| fish polar lipids retard atherosclerosis in rabbits by down-regulating paf biosynthesis and up-regulating paf catabolism. | platelet activating factor (paf) has been proposed as a key factor and initial trigger in atherosclerosis. recently, a modulation of paf metabolism by bioactive food constituents has been suggested. in this study we investigated the effect of fish polar lipid consumption on paf metabolism. | 2011 | 22087726 |
| Post-larval development of the microcotylid monogenean Sparicotyle chrysophrii (Van Beneden and Hesse, 1863): comparison with species of Microcotylidae and Heteraxinidae. | The chronology of post-larval development in S. chrysophrii, a polyopisthocotylean monogenean parasite of the gilthead seabream (Sparus aurata L.), was experimentally studied. It is compared with other species within the Microcotylidae and the Heteraxinidae, including an analysis of the changes in attachment and the growth rate. Gilthead seabreams infected by larvae of S. chrysophrii were killed periodically in order to collect the different developmental stages. Parasite total body length, hapt ... | 2011 | 22015332 |
| Discovery of novel biopreservation agents with inhibitory effects on growth of food-borne pathogens and their application to seafood products. | Selection of protective cultures is relevant in order to biopreserve and improve the functional safety of food products, mainly through inhibition of spoilage and/or pathogenic bacteria. Accordingly, the present study investigated potential applications of lactic acid bacteria (LAB) in the biopreservation of fish and shellfish products. For this purpose, a collection of 84 LAB strains isolated from sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) was identified and characterized for ... | 2011 | 22041547 |
| immunocytochemical and ultrastructural characterization of coexistence of pancreatic polypeptide and glucagon-like immunoreactivity in the pancreatic endocrine cells of sparus auratus l. (teleostei). | coexistence of pancreatic polypeptide (pp)- and glucagon-like immunoreactivity was demonstrated in the pancreatic endocrine cells of the teleost fish sparus auratus. an immunofluorescence double-staining method revealed coexistence of glucagon- and pp-like immunoreactivity in endocrine cells of small and intermediate islets. in contrast to small islets, the intermediate ones also contained a variable number of glucagon-immunoreactive cells next to cells having both immunoreactivities. coexistenc ... | 2011 | 3131188 |
| cyclic histological changes in the pituitary gland of sparus aurata in correlation to the gonadal cycle. | | 2012 | 3410381 |
| an immunocytochemical and ultrastructural study of the endocrine pancreas of sparus auratus l. (teleostei). | the pancreatic endocrine cells of sparus auratus (gilthead sea bream) are concentrated in two or three principal islets, or brockmann bodies, and numerous smaller islets embedded in the exocrine tissue. insulin-, glucagon-, somatostatin-, and pancreatic polypeptide (pp)-immunoreactive cells were identified in all pancreatic islets of s. auratus using an indirect immunocytochemical (pap) method. insulin-immunoreactive cells were found in the central region of the islets. glucagon-immunoreactive c ... | 2012 | 3549431 |
| microbiological evolution of gilthead sea bream (sparus aurata) in canary islands during ice storage. | this study analyses the microbiological changes with traditional methods for total mesophilic aerobic, psychrotrophic, aeromonas sp., pseudomonas sp., shewanella putrefaciens, enterobacteriaceae, sulfide-reducing clostridium and photobacterium phosphoreum in muscle, skin and gills of whole ungutted gilthead sea bream (sparus aurata) stored in ice during 18 days. the muscle tissue showed the minor grade of contamination, followed by the skin and the gills, with statistic significance (p < 0.001). ... | 2013 | 25745228 |
| preliminary investigation on the use of allyi isothiocyanate to increase the shelf-life of gilthead sea bream (sparus aurata) fillets. | the aim of this work is to evaluate the activity of allyl isothiocyanate (aitc) against fish spoilage bacteria (specific spoilage organisms; ssos) as well as its possible use in gilthead sea bream (sparus aurata) fillets to extend their shelf-life. in this regard, in vitro tests are carried out in order to evaluate the inhibitory activity of aitc and its vapours on several strains of ssos. the aitc effect on the shelf-life of sea bream fillets was made by putting them in plastic trays hermetical ... | 2015 | 27800402 |
| characterization of muscle-regulatory gene, myod, from flounder (paralichthys olivaceus) and analysis of its expression patterns during embryogenesis. | specification and differentiation of skeletal muscle cells are driven by the activity of genes encoding members of the myogenic regulatory factors (mrfs). in vertebrates, the mrf family includes myod, myf5, myogenin, and mrf4. the mrfs are capable of converting a variety of nonmuscle cells into myoblasts and myotubes. to better understand their roles in fish muscle development, we isolated the myod gene from flounder (paralichthys olivaceus) and analyzed its structure and patterns of expression. ... | 2006 | 16362809 |
| pept1 mrna expression levels in sea bream (sparus aurata) fed different plant protein sources. | the expression and regulation of intestinal oligopeptide transporter (pept)-1 when vegetable sources are used as a substitute for fish meal in the diet of marine fish has not yet been explored. in the present study, as part of our ongoing work on elucidating pept1 gene expression in relation to different dietary treatments, we have now isolated and deposited in genbank database (accession no. gu733710) a cdna sequence representing the pept1 in the sea bream (sparus aurata). the "de novo" predict ... | 2013 | 23449729 |
| caspase-1 and il-1β processing in a teleost fish. | interleukine-1β (il-1β) is the most studied pro-inflammatory cytokine, playing a central role in the generation of systemic and local responses to infection, injury, and immunological challenges. in mammals, il-1β is synthesized as an inactive 31 kda precursor that is cleaved by caspase-1 generating a 17.5 kda secreted active mature form. the caspase-1 cleavage site strictly conserved in all mammalian il-1β sequences is absent in il-1β sequences reported for non-mammalian vertebrates. recently, ... | 2012 | 23226286 |
| survival of vibrio parahaemolyticus and aeromonas hydrophila in sea bream (sparus aurata) fillets packaged under enriched co(2) modified atmospheres. | the ability to survive of two pathogens (vibrio parahaemolyticus and aeromonas hydrophila) spread over sea bream fillets packaged under different modified atmospheres (maps) was studied at 0°c and 4°c under refrigerated storage. the atmospheres used were 60% co2/40% n2, 70% co2/30% n2 and 80% co2/20% n2 and a control batch packaged in air. head space gas analyses, microbial counts and confirming test of pathogenic bacteria were carried out during 16days. the results obtained showed that all the ... | 2013 | 23860282 |
| effects of enteromyxum leei (myxozoa) infection on gilthead sea bream (sparus aurata) (teleostei) intestinal mucus: glycoprotein profile and bacterial adhesion. | the intestinal myxosporean parasite enteromyxum leei causes severe desquamative enteritis in gilthead sea bream (sparus aurata) (teleostei) that impairs nutrient absorption causing anorexia and cachexia. in fish, as in terrestrial vertebrates, intestinal goblet cells are responsible for the adherent mucus secretion overlying epithelial cells, which constitutes a first line of innate immune defense against offending microorganisms but serves also as substrate and nutrient source for the commensal ... | 2013 | 23086443 |
| preliminary study on the in vitro and in vivo effects of asparagopsis taxiformis bioactive phycoderivates on teleosts. | several compounds from marine organisms have been studied for their potential use in aquaculture. among the red algae, asparagopsis taxiformis is considered one of the most promising species for the production of bioactive metabolites with numerous proposed applications. here, the in vitro antibacterial activity, the easy handling and the absence of adverse effects on marine fish species are reported. depending on the seasonal period of sampling, ethanol extracts of a. taxiformis exhibited signi ... | 2016 | 27826246 |
| lipopolysaccharides isolated from aeromonas salmonicida and vibrio anguillarum show quantitative but not qualitative differences in inflammatory outcome in sparus aurata (gilthead seabream). | in fish, the defence system recognises pathogenic microorganisms via pathogen recognition receptors (prrs) that sense particular structures of the pathogens; the so-called pathogen associated molecular patterns (pamps) such as bacterial lipopolysaccharides (lpss). the result of the pamp-prr interactions leads to complex and orchestrated immune responses. in this study, sparus aurata (gilthead seabream) were intraperitoneally injected with purified lipopolysaccharide (lps) from aeromonas salmonic ... | 2014 | 24954838 |
| stress and humoral innate immune response of gilthead seabream sparus aurata cultured in sea cages. | innate and acquired immune responses of gilthead seabream sparus aurata was studied under normal culture and short-term stressful conditions for 18 months in offshore sea cages in alghero bay, italy. every 45 d, 50 fish were sampled and divided into two groups: fish in the first group (normal culture conditions) were bled after harvesting; fish in the second group were put into a tank under stressful conditions (crowding and confinement) and bled after 2 h. innate humoral immunity, such as compl ... | 2016 | 27485027 |
| detection and characterization of antimicrobial resistance and putative virulence genes in aeromonas veronii biovar sobria isolated from gilthead sea bream (sparus aurata l.). | the purposes of this study were to investigate the antimicrobial resistance of aeromonas veronii biovar sobria isolated from gilthead sea bream and to characterize the virulence-implicated genes. fish samples (n=365) were collected from wholesale and retail markets in aljouf, saudi arabia between 2013 and 2014. a total of 45 a. veronii biovar sobria isolates (12.3%) from those samples were tested for resistance to a range of antimicrobial agents. all strains exhibited 100% resistances to nalidix ... | 2015 | 26218714 |
| draft genome sequence of aliivibrio fischeri strain 5lc, a bacterium retrieved from gilthead sea bream (sparus aurata) larvae reared in aquaculture. | to shed light on the putative host-mediated lifestyle of the quintessential marine symbiont aliivibrio fischeri, and on the symbiosis versus potentially pathogenic features of bacteria associated with farmed fish, we report the draft genome sequence of a. fischeri strain 5lc, a bacterium retrieved from gilthead sea bream (sparus aurata) larvae. | 2015 | 26044435 |