| radical scavenging and reducing ability of tilapia (oreochromis niloticus) protein hydrolysates. | enzymatically hydrolyzed fish protein hydrolysates could be used as a source of antioxidative nutraceuticals. in our current work, we have investigated alkali-solubilized tilapia ( oreochromis niloticus) protein hydrolysates for their ability to scavenge reactive oxygen species (ros) and for their reducing power. tilapia protein isolate was prepared by an alkaline solubilization technique and used as a substrate for enzyme hydrolysis. cryotin, protease a 'amano' 2, protease n 'amano', neutrase a ... | 2008 | 18828605 |
| trace metal concentrations in nile tilapia (oreochromis niloticus) in three catchments, sri lanka. | samples of the muscle and liver of the nile tilapia (oreochromis niloticus) were obtained from a single reservoir in each of three sri lankan catchments (kaudulla, rajanganaya, and udawalawe reservoirs in the mahaweli, kala oya, and walawe ganga river basins, respectively) in 2002. the concentrations of 12 elements were consistently detected in the tilapia muscle and liver (ca, cd, cu, fe, hg, k, mg, mn, na, p, sr and zn). however, a three factorial principal components analysis suggested that t ... | 2009 | 18949439 |
| accumulation of heavy metals in freshwater fish in cage aquaculture at cirata reservoir, west java, indonesia. | freshwater fish demand in west java is supported by cage aquaculture in reservoirs. cirata reservoir is one of three cascading hydropower reservoirs built along the citarum river that receives domestic and industrial wastes. water pollution by heavy metals, such as copper and lead, increases the health risk of humans who consume the fish. the study was aimed to evaluate the concentration of copper and lead in fish, specifically on cyprinus carpio, that are widely cultivated in the cirata reservo ... | 2008 | 18991927 |
| aspects of the natural history and virulence of s. agalactiae infection in nile tilapia. | streptococcus agalactiae is an emerging pathogen in nile tilapia (oreochromis niloticus) worldwide. to investigate aspects of the epidemiology, transmission and virulence of s. agalactiae infections, nine outbreaks of meningoencephalitis and septicemia in nile tilapia farms in brazil were analyzed. records from the outbreaks revealed large variation in the weight of fish affected, high mortality, and disease occurrence at water temperatures above 26 degrees c. s. agalactiae was isolated from dis ... | 2009 | 19042097 |
| time-dependent protective efficacy of trolox (vitamin e analog) against microcystin-induced toxicity in tilapia (oreochromis niloticus). | microcystins (mcs), hepatotoxins from cyanobacteria, induce oxidative stress and pathological changes in fish that can be ameliorated with chemoprotectants such as vitamin e (vit e). this study investigated the time period after mcs exposure in which trolox, a vitamin e analog, is effective against oxidative and histological damage in different organs of tilapia (oreochromis niloticus). fish were fed trolox supplement (700 mg/kg diet) for 7 days, or received only commercial fish food, and then w ... | 2009 | 19051281 |
| reproductive strategy of oreochromis niloticus (pisces: cichlidae) in opa reservoir, ile-ife, nigeria. | the fish family cichlidae has a large diversity and dominates african freshwater bodies, with over 200 species reported in inland waters. sampling for the fish oreochromis niloticus (linnaeus) in opa reservoir, nigeria, started in october 1997 and extended until february 2000. the fishing methods employed for collecting the 1 430 specimens were cast netting and gillnetting. egg diameter varied between 2.12 mm and 2.69 mm with a mean of 2.47+/-0.02. female gonadosomatic index was 1.34+/-0.01 (0.1 ... | 2007 | 19069769 |
| induction of cytochrome p450 1a1 as a biomarker of benzo-a-pyrene pollution in egyptian fresh water fish. | the activity of ethoxyresorufin-o-dealkylase (erod) in the liver of oreochromis niloticus and clarias gariepinus was evaluated as a response to experimental and natural contamination of water with benzo-a-pyrene and/or cadmium. the activity was measured fluorimetrically in the hepatic s9 fraction while the content of the enzyme was measured by elisa. the response appeared as early as six hours post exposure. this study also reveled that oreochromis niloticus exhibits higher values of erod activi ... | 2007 | 19069910 |
| effects of dietary selenium on the oxidative stress and pathological changes in tilapia (oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom. | the present study investigates the role of selenium (se) supplementation (as sodium selenite) on the oxidative stress and histopathological changes induced by cyanobacterial cells containing microcystins (mcs) in tilapia fish (oreochromis niloticus). variation in lipid peroxidation (lpo) levels and carbonyl groups content, reduced glutathione/oxidized glutathione (gsh/gssg) ratio, and catalase (cat), superoxide dismutase (sod), glutathione reductase (gr), glutathione peroxidase (gpx) and glutath ... | 2009 | 19073206 |
| occurrence of a novel acetylated amino acid, n(alpha)-acetylhistidine, in skeletal muscle of freshwater fish and other ectothermic vertebrates. | the occurrence of n(alpha)-acetylhistidine (nah) in skeletal muscle of 91 species of freshwater fish and 9 species of other ectothermic vertebrates was investigated, with consideration of phylogenetic relationships. of the 91 freshwater fish species examined, 13 species (7 cichlids, 5 anabantids, and 1 catfish) contained considerable amounts (>1 micromol/g) of nah in their skeletal muscles. the highest level (10.37 micromol/g) of nah was found in the tissue of betta splendens (siamese fighting f ... | 2009 | 19100335 |
| potential of psidium guajava supplemented fish diets in controlling aeromonas hydrophila infection in tilapia (oreochromis niloticus). | fourteen herbs were extracted in water, 95% ethanol, and ether, and tested in vitro for antimicrobial activities against aeromonas hydrophila, a fish pathogen causing motile aeromonas septicemia. using swab paper disc assays and minimal inhibitory concentration (mic) determinations, we noted that the ethanol extract of psidium guajava leaf exhibited the strongest antimicrobial activity. the extract allowed growth-inhibited a. hydrophila cells to regrow in fresh bhi broth indicating a bacteriosta ... | 2008 | 19111636 |
| angling sport fishing in lobo-broa reservoir (itirapina, sp, brazil). | the objective of this paper was to study angling from september, 2002 to september, 2004 in the lobo-broa reservoir, itirapina, sp (22 degrees 15' s and 47 degrees 49' w). interviews (total 1,027) with sport fishers were accomplished in the three main fishing sites (horto, píer and praia). this fishing was practiced with a simple fishing rod and reel, mainly in horto, where the catches and fishing effort were higher. the catches were mainly composed of cichlidae (geophagus brasiliensis, oreochro ... | 2008 | 19197489 |
| usage of putative zebrafish u6 promoters to express shrna in nile tilapia and shrimp cell extracts. | we conducted in vitro transcription activities of the three zebrafish u6 putative promoters across species in cell extracts prepared from nile tilapia (oreochromis niloticus) and shrimps. the transcription efficiency of these putative u6 promoters in nile tilapia cell extracts was similar to that of zebrafish cell extracts. in addition, all three zebrafish u6 snrna promoters were able to express the shrna in cell extracts prepared from two shrimp species, penaeus monodon and litopenaeus vannamei ... | 2009 | 19225901 |
| age and growth of the tilapia, oreochromis niloticus (perciformes: cichlidae) from a tropical shallow lake in mexico. | age and growth of nile tilapia (oreochromis niloticus) were determined using 1039 specimens collected in a tropical shallow lake of mexico, from january to december 1993. standard length of females ranged from 9.0 to 16.5 cm and males from 8.9 to 14.8 cm. the standard length-weight relationships for all individuals was bw=0.1207 sl2469. the age data, derived from opercular bone readings, were used to estimate the growth parameters of the von bertalanffy equation: lx=17.88 cm, k=0.3409, t0=-1.543 ... | 2008 | 19256450 |
| effects of dietary n-acetylcysteine on the oxidative stress induced in tilapia (oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom. | fish can be exposed to toxic cyanobacterial cells in natural waters and fish farms and suffer from oxidative damage. the present study investigates the effects of n-acetylcysteine (nac), a glutathione (gsh) precursor, on the oxidative stress induced by microcystis cyanobacterial cells containing microcystins (mcs) in tilapia fish (oreochromis niloticus). variation in lipid peroxidation (lpo) levels, carbonyl group content, reduced glutathione to oxidized glutathione ratio (gsh:gssg), and catalas ... | 2009 | 19290682 |
| micrococcus luteus and pseudomonas species as probiotics for promoting the growth performance and health of nile tilapia, oreochromis niloticus. | micrococcus luteus and pseudomonas species were isolated from the gonads and intestine of nile tilapia, oreochromis niloticus. m. luteus and ps. species antagonized aeromonas hydrophila with inhibition zone of 4 and 9 cm diameter, respectively. both microorganisms were added to artificial basal diet with 30% crude protein to evaluate their efficacy on the growth-performance and survival rate, besides some blood-parameters and chemistry. two hundred and forty o. niloticus with average body-weight ... | 2009 | 19361560 |
| effect of treatment with probiotics as water additives on tilapia (oreochromis niloticus) growth performance and immune response. | a feeding trial was conducted for 40 days to delineate the effect of treatment with probiotics as water additives on tilapia (oreochromis niloticus) growth performance and immune response. about 360 juveniles were randomly distributed into four treatment groups, each with three replicates. different probiotics (t-1, bacillus subtilis b10; t-2, bacillus coagulans b16; t-3, rhodopseudomonas palustris g06) were added to the water of tanks at final concentration of 1 x 10(7) cfu ml(-1) every 2 days, ... | 2010 | 19363655 |
| amino acid requirement studies in oreochromis niloticus by application of principles of the diet dilution technique. | data analysis utilized four growth experiments with mixed diets limiting in lysine, in threonine, and in methionine respectively. all male juvenile orechromis niloticus [12 g average body weight instead of average (bw) at start, four repetition tanks per diet, 56 days experimental period] provided the database for application of an exponential n-utilization model. imposing amino acid efficiency data were utilized for modelling of amino acid requirements depending on the level of daily protein de ... | 2009 | 19364385 |
| effects of dietary cyanobacteria of two different sources on growth and recovery of hybrid tilapia (oreochromis niloticus x o. aureus). | a 115 days feeding trial was conducted to evaluate the effect of dietary cyanobacteria on growth, microcystins (mcs) accumulation in hybrid tilapia (oreochromis niloticusxo. aureus) and the recovery when the fish were free of cyanobacteria. three experimental diets were formulated: the control (cyanobacteria free diet); one test diet with cyanobacteria from lake taihu (amt, 80.0 microg mcs g(-1) diet) and one with cyanobacteria from lake dianchi (amd, 410.0 microg mcs g(-1) diet). each diet was ... | 2009 | 19374913 |
| prophylactic effect of andrographis paniculata extracts against streptococcus agalactiae infection in nile tilapia (oreochromis niloticus). | six herbs were assessed for their antimicrobial activity against streptococcus agalactiae, a major fish pathogen causing streptococcosis. each herb was extracted with 3 solvents: water, 95% ethanol, and methanol. using swab paper disc assays, aqueous extracts of andrographis paniculata and allium sativum produced the largest (27.5 mm) and smallest (10.3 mm) inhibition zones, respectively. determination of minimal inhibitory concentration (mic) of herb extracts against s. agalactiae showed that t ... | 2009 | 19393561 |
| human streptococcus agalactiae isolate in nile tilapia (oreochromis niloticus). | streptococcus agalactiae, the lancefield group b streptococcus (gbs) long recognized as a mammalian pathogen, is an emerging concern with regard to fish. we show that a gbs serotype ia multilocus sequence type st-7 isolate from a clinical case of human neonatal meningitis caused disease and death in nile tilapia (oreochromis niloticus). | 2009 | 19402966 |
| diplostomiasis in cultured and wild tilapia oreochromis niloticus in guerrero state, mexico. | this paper is a comparative study of diplostomum (austrodiplostomum) compactum (lutz, 1928) in nile tilapia oreochromis niloticus (linneo) from two fish farms and two nearby coastal lagoons in guerrero state, mexico. the higher infections levels in cultured tilapia than wild tilapia is attributed to higher fish densities in the culture systems and higher abundance of the snail biomphalaria cf. havanensis (pteiffer), first intermediate host of this parasite in freshwater and brackish water system ... | 2009 | 19452167 |
| lack of constitutive and inducible ethoxyresorufin-o-deethylase activity in the liver of suckermouth armored catfish (hypostomus affinis and hypostomus auroguttatus, loricariidae). | we investigated the presence and inducibility of cyp1a in suckermouth catfish (hypostomus affinis and hypostomus auroguttatus, loricariidae), tilapia (oreochromis niloticus, cichlidae) and mice (mus musculus, muridae). alkoxyresorufin-o-dealkylases (erod, mrod, prod and brod) were detected and proved to be inducible (beta-naphthoflavone, bnf or dimethylbenz[a]anthracene, dmba, 50 mg/kg bw ip) in liver microsomes from tilapia and mice. in loricariids, alkoxyresorufin-o-dealkylases were either und ... | 2009 | 19460462 |
| sediment genotoxicity in the tietê river (são paulo, brazil): in vitro comet assay versus in situ micronucleus assay studies. | the in vitro comet assay with the permanent fish cell line rtl-w1 and the in situ micronucleus assay using erythrocytes from indigenous tilapia (oreochromis niloticus) were used to detect genotoxicity in tietê river sediments (são paulo, brazil). either test was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. discharges from são paulo city have ... | 2009 | 19477010 |
| orexin-b-like immunoreactivity localized in both luteinizing hormone- and thyroid-stimulating hormone-containing cells in the nile tilapia (oreochromis niloticus) pituitary. | immunohistochemical techniques were employed to examine orexin-like immunoreactivities in the pituitary of the nile tilapia (oreochromis niloticus). rabbit anti-orexin-a serum and mouse anti-orexin-b monoclonal antibodies were used as primary antibodies. orexin-b immunoreactive cells corresponded to luteinizing hormone (lh)- or thyroid-stimulating hormone (tsh)-containing cells, and all lh- and tsh-containing cells were immunoreactive for orexin-b. however, we found no orexin-a immunoreactive ce ... | 2009 | 18656218 |
| outbreak of betanodavirus infection in tilapia, oreochromis niloticus (l.), in fresh water. | a betanodavirus associated with a massive mortality was isolated from larvae of tilapia, oreochromis niloticus, maintained in fresh water at 30 degrees c. histopathology revealed vacuolation of the nervous system, suggesting an infection by a betanodavirus. the virus was identified by indirect fluorescent antibody test in the ssn1 cell line and further characterized by sequencing of a pcr product. sequencing of the t4 region of the coat protein gene indicated a phylogenetic clustering of this is ... | 2009 | 19500206 |
| [the influence of branchial parasitism by monogenoid trematodes on the development of nile tilapia (oreochromis niloticus) linnaeus, 1757 bred in net-pond systems in capivara dam, pr]. | tilapias are fish originally from africa which nowadays are commercially bred in almost 100 countries, being one of the most commercially bred species in the world. in this work the trematode population of the monogenoidea group present in the branchiae of nile tilapias bred in 4 net-ponds with volume of 4 m3 each, was monitored during 5 months. the juvenile fish, presenting initial average weight of 37.65 g originated from other piscicultures, were stocked in the density of 250 animals.m(-3) an ... | 2009 | 19500461 |
| francisella sp., an emerging pathogen of tilapia, oreochromis niloticus (l.), in costa rica. | francisella sp. is an emergent bacterial pathogen that causes acute to chronic disease in warm and cold water cultured and wild fish species. during the past 3 years, the bacterium has been detected in tilapia, oreochromis niloticus, cultured in costa rica. infected fish presented non-specific clinical signs, such as erratic swimming, anorexia, anaemia, exophthalmia and high mortality. upon macroscopic and microscopic examination, several internal organs (mainly spleen and kidney) were enlarged ... | 2009 | 19515205 |
| kinetics of lactobacillus plantarum 44a in the faeces of tilapia (oreochromis niloticus) after its intake in feed. | to study the kinetic passage of lactobacillus plantarum 44a from feed to faeces of tilapia in order to calculate the number of lactobacillus excreted. | 2009 | 19523147 |
| first isolation and characterization of lactococcus garvieae from brazilian nile tilapia, oreochromis niloticus (l.), and pintado, pseudoplathystoma corruscans (spix & agassiz). | lactococcus garvieae infection in cultured nile tilapia, oreochromis niloticus (l.), and pintado, pseudoplathystoma corruscans (spix & agassiz), from brazil is reported. the commercial bacterial identification system, biolog microlog, confirmed the identity of l. garvieae. infectivity trials conducted in nile tilapia using brazilian nile tilapia l. garvieae isolates resulted in a median lethal dose-50 of 1.4 x 10(5) colony-forming units (cfu)/fish. this is the first evidence of the presence of t ... | 2009 | 19531061 |
| saxitoxins accumulation by freshwater tilapia (oreochromis niloticus) for human consumption. | the accumulation of saxitoxins (stxs) in fish from freshwater aquaculture was investigated for the first time in the present study. cyanotoxins have been monitored in liver and muscle samples of oreochromis niloticus by chromatographic methods, both before and after the depuration process. the results show that tilapia can accumulate stxs. our findings suggest that depuration with clean water is an alternative process to eliminate stxs from fish and, therefore, improve the safety of tilapia for ... | 2009 | 19560484 |
| antagonism of aeromonas hydrophila by propolis and its effect on the performance of nile tilapia, oreochromis niloticus. | propolis, a resinous substance collected by apis mellifera bees from various plant sources and mixed with secreted beeswax, is a multifunctional material used by bees in the construction, maintenance, and protection of their hives. the collected propolis sample, from high egypt, was dark-green with olive-odor. the minimal inhibition concentration (mic) of propolis-ethanolic-extract, against aeromonas hydrophila, was 80 microg propolis-ethanolic-extract and crude propolis (1%) were added to artif ... | 2009 | 19563896 |
| identification of (l)-fucose-binding proteins from the nile tilapia (oreochromis niloticus l.) serum. | lectins are carbohydrate-binding proteins with many biological functions including cellular recognition and innate immunity. in this study, a major l-fucose-binding lectin from the serum of nile tilapia (oreochromis niloticus l.), designated as tfbp, was isolated by l-fucose-bsa sepharose cl6b affinity chromatography. the sds-page (10%) analysis of tfbp revealed a major band of approximately 23 kda with an n-terminal amino acid sequence of dqtetagqqsxpqdihavlrel which did not give significant si ... | 2009 | 19563899 |
| assessment of cyanobacteria toxins in freshwater fish: a case study of murchison bay (lake victoria) and lake mburo, uganda. | there is little information on the distribution of microcystins (mcs) in oreochromis niloticus (on) and lates niloticus (ln) obtained from l. mburo and murchison bay of l. victoria. these fishes are harvested and sold both for local human consumption and for export. the presence of mc-rr, mc-lr and mc-yr in different organs (gut, muscle and liver) was determined using liquid chromatography coupled with a mass spectrometry detector (lc/ms/ms). the total mcs in on gut, muscle and liver were 1312.0 ... | 2010 | 19646467 |
| identification of some skeletal deformities in freshwater teleosts raised in egyptian aquaculture. | this study examines different forms of skeletal deformities detected in fish species collected from two egyptian aquaculture facilities during two harvest seasons in 2008. various patterns of skeletal deformities were observed in 19 of 959 fish collected. deformities were diagnosed using a number of techniques including clinical, radiographic, sonographic and histopathological evaluations. observed deformities included: lordosis and kyphosis in african catfish (clarius gariepenius); lateral proj ... | 2009 | 19647852 |
| cloning and molecular characterization of the glucose transporter 1 in tilapia (oreochromis niloticus). | facilitative glucose transporters (gluts) are responsible for passively transporting monosaccharides across the plasma membrane. we sequenced and characterized the nile tilapia (oreochromis niloticus) glut-1 (tglut-1) cdna and genomic dna. using rapid amplification of the cdna ends (race), two tglut-1 transcripts were detected differing in the length of the 3' untranslated region, 2851 and 4577 bp. translated tglut-1 is a 490 amino acid product, which shares 74% homology with that of humans. com ... | 2010 | 19651126 |
| genotyping of streptococcus agalactiae strains isolated from fish, human and cattle and their virulence potential in nile tilapia. | streptococcus agalactiae (lancefield group b; gbs) is a pathogen that causes meningoencephalitis in fish, mastitis in cows, and neonatal sepsis in humans. the objective of this study was to characterize s. agalactiae isolated from fish (n=27), cows (n=9), and humans (n=10) using pulsed-field gel electrophoresis (pfge) and to investigate the virulence of the identified strains in nile tilapia (oreochromis niloticus). the pfge types were determined by dendogram analyses and the in vivo virulence w ... | 2010 | 19726142 |
| protective effect of clove oil-supplemented fish diets on experimental lactococcus garvieae infection in tilapia. | the essential oils extracted from the four herbs, cinnamon (cinnamomum verum), clove (syzygium aromaticum), ginger (zingiber officinale) and holy basil (ocimum sanctum), were investigated for their antimicrobial activity and mode of action against lactococcus garvieae, a fish pathogenic bacteria causing lactococcosis. of all the tested oils, clove oil had the strongest inhibitory effect and exhibited a bactericidal mode of action against the pathogenic bacterium. when an intraperitoneal infectio ... | 2009 | 19734665 |
| enhanced mortality in nile tilapia oreochromis niloticus following coinfections with ichthyophthiriasis and streptococcosis. | ichthyophthirius multifiliis fouquet (ich) and streptococcus iniae are 2 major pathogens of cultured nile tilapia oreochromis niloticus (l). currently there is no information available for the effect of coinfection by ich and s. iniae on fish. the objective of this study was to determine the effects of parasite load and ich development size on fish mortality following s. iniae infection. low mortality (< or =20%) was observed in tilapia exposed to ich or s. iniae alone. mortalities increased fro ... | 2009 | 19750806 |
| ichthyophthirius multifiliis infection induces apoptosis in different species of tilapia. | the parasitic ciliate ichthyophthirius multifiliis (ich) is one of the most important protozoan pathogens of freshwater fish worldwide. examination of 4 species of tilapia from the river nile recorded highest rate infection in oreochromis niloticus, followed by oreochromis aureus, sarotherodon galilaeus and tilapia zilli. by electron microscopy, apoptotic cells collected from tilapia species infected with "ich" showed an aggregation on the apical and basal parts of the ciliated and non-ciliated ... | 2009 | 19795773 |
| genotoxicity of two pathogenic strains of zoosporic fungi (achlya klebsiana and aphanomyces laevis) on erythrocytes of nile tilapia oreochromis niloticus niloticus. | in the present work we have described the genotoxic potential of two pathogenic strains of zoosporic fungi (achlya klebsiana and aphanomyces laevis) on erythrocytes of nile tilapia oreochromis niloticus niloticus for the first time by three complementary tests: micronucleus test (mn), nuclear lesions (nl) and comet assay (ca). the groups exposed to the zoosporic fungi subjected to the mn and nl test showed statistically significant differences in mn and nl frequencies with respect to the control ... | 2010 | 19811832 |
| [experimental establishment of life cycle of clonorchis sinensis]. | to establish and maintain the life cycle of clonorchis sinensis in laboratory. | 2009 | 19856506 |
| antioxidant enzyme activity and lipid peroxidation in liver and gill tissues of nile tilapia (oreochromis niloticus) following in vivo exposure to domoic acid. | domoic acid (da) is a neurotoxicant produced by pseudo-nitzschia from diatomeae. although the neurotoxic and genotoxic effects of da have been well documented, the number of in vivo studies regarding the oxidative stress inducing effects of da is quite limited. in this study, in vivo toxic effects of da were investigated on fish oreochromis niloticus (fam: cichlidae), using oxidative stress biomarkers. fish were exposed to three different concentrations (1, 5 and 10 microg/g body weight) of da v ... | 2010 | 19931299 |
| attenuation of the fish pathogen francisella sp. by mutation of the iglc* gene. | fish francisellosis is an emergent disease caused by gram-negative facultative intracellular bacteria of the genus francisella. different strains of the bacterium have caused high mortalities in warmwater and coldwater fish species. francisella sp. isolates from fish have been found to share more than 97% identity to the human pathogen francisella tularensis upon 16s ribosomal rna sequence comparison. homologue genes of the f. tularensis intracellular growth locus (igla*, iglb*, iglc*, and igld* ... | 2009 | 20043398 |
| effects of zinc and cadmium on erythrocyte antioxidant systems of a freshwater fish oreochromis niloticus. | in this work to determine the effects of metals exposure of oreochromis niloticus on erythrocyte antioxidant systems, fish were exposed to 5.0 mg/l zn, 1.0 mg/l cd, and 5.0 mg/l zn + 1.0 mg/l cd mixtures for 7 and 14 days and reduced glutathione (gsh) level, catalase (cat), and glucose-6-phosphate dehydrogenase (g6pd) activities were investigated. in addition, zn or cd levels in whole blood were studied. erythrocyte gsh level and cat and g6pd enzyme activities increased in response to single and ... | 2010 | 20143450 |
| interaction of francisella asiatica with tilapia (oreochromis niloticus) innate immunity. | members of the genus francisella are facultative intracellular bacteria that cause important diseases in a wide variety of animals worldwide, including humans and fish. several genes that are important for intramacrophage survival have been identified, including the iglc gene, which is found in the iglabcd operon in the francisella sp. pathogenicity island (fpi). in the present study, we examined the interaction of wild-type francisella asiatica and a delta iglc mutant strain with fish serum and ... | 2010 | 20160018 |
| first report of methicillin-resistant staphylococcus aureus from cage-cultured tilapia (oreochromis niloticus). | swabs from the brain, eyes and kidneys of tilapia from 11 farms were collected for a period of 2 years. they were grown on blood agar before cultures of suspected staphylococcus aureus were subjected to abi staph detection kit and pcr for identification. they were then grown on oxacillin resistance screening agar base (orsab) and subjected to pcr using the mrsa 17 kb forward and reverse primers to identify the methicillin-resistant s. aureus (mrsa). a total of 559 isolates of staphylococcus spp. ... | 2010 | 20189324 |
| protection against heterologous streptococcus iniae isolates using a modified bacterin vaccine in nile tilapia, oreochromis niloticus (l.). | streptococcus iniae is a significant pathogen impacting aquaculture production worldwide. the objectives of this study were to determine whether a developed modified s. iniae (ars-98-60) bacterin vaccine is efficacious in nile tilapia, oreochromis niloticus (l.), against challenge with heterologous isolates from diverse geographical locations and to evaluate protein and antigenic variability among the isolates tested. two groups of tilapia (approximately 5 g) were intraperitoneally (ip) vaccinat ... | 2010 | 20298447 |
| the effect of pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (oreochromis niloticus). | aim: to assess pediococcus acidilactici as a dietary supplement for on-growing red tilapia (oreochromis niloticus). methods and results: tilapia were fed either a control diet or control diet supplemented with ped. acidilactici at 10(7) cfu g(-1) for 32 days. ped. acidilactici colonized the intestinal tract and significantly affected the intestinal microbial communities. pcr-dgge revealed direct antagonism of gastric ped. acidilactici with an endogenous uncultured bacterium during a period of re ... | 2010 | 20353430 |
| effect of dietary supplementation of inulin and vitamin c on the growth, hematology, innate immunity, and resistance of nile tilapia (oreochromis niloticus). | the in vivo activities of inulin and ascorbic acid were evaluated experimentally via using 450 nile tilapia (oreochromis niloticus) that were distributed into 3 equal groups (each of three replicates). fish of the first group served as a control and received a balanced diet free from inulin and vitamin c. the second fed on balanced diet supplemented with inulin (5 g kg(-1)), whereas, the third one received a balanced diet supplemented with vitamin c (500 mg kg(-1)). the survival and growth perfo ... | 2010 | 20371294 |
| first description of the adult stage of clinostomum cutaneum paperna, 1964 (digenea: clinostomidae) from grey herons ardea cinerea l. and a redescription of the metacercaria from the nile tilapia oreochromis niloticus niloticus (l.) in kenya. | the combined use of morphological and molecular studies allowed for the first time the recognition and description of the adult stage of clinostomum cutaneum paperna, 1964 from the grey heron ardea cinerea l. in kenya. a redescription of the metacercaria that infect nile tilapia oreochromis niloticus niloticus (l.) from the same aquatic environment is also presented. c. cutaneum differs from all other species of clinostomum leidy, 1856 in the shape of its uterus. sequencing the rrna confirmed th ... | 2010 | 20401577 |
| a commercial rapid optical immunoassay detects streptococcus agalactiae from aquatic cultures and clinical specimens. | the biostar strep b optical immunoassay (strep b oia) (biostar oia strep b assay kit; biostar incorporation, louisville, co, usa), commonly used for diagnosis of human maternal group b streptococcus (gbs) colonization, was evaluated for its diagnostic and analytical sensitivity and specificity to aquatic animal gbs isolates, cross-reactivity, and diagnosis and recovery of gbs directly from clinically- infected fish swabs. strep b oia identified 25 known fish and dolphin gbs isolates. thirteen no ... | 2010 | 20430538 |
| oxidative stress and antioxidant enzymes in liver and white muscle of nile tilapia juveniles in chronic ammonia exposure. | oxidative stress and antioxidant enzyme activities in liver and white muscle of nile tilapia (oreochromis niloticus) juveniles (10+/-1.2g) in chronic exposure to sublethal total ammonia nitrogen (tan) were studied. the fish were exposed to the tan concentrations, 5 mg l(-1) (low) or 10 mg l(-1) (high) for consecutive 70 days at 26+/-0.5 degrees c temperature. at the end of experimental period, lipid peroxidation and protein carbonylation levels and the activities of xanthine oxidase (xo), aldehy ... | 2010 | 20434782 |
| evaluation of genotoxicity from nilufer stream (bursa/turkey) water using piscine micronucleus test. | in the present study, the in vivo micronucleus (mn) test in fish erythrocytes was used to evaluate the genotoxic potentials of water samples collected from four different sites along the nilufer stream which empties into the marmara sea on the north-west of turkey. nilufer stream receives discharges of industrial and domestic wastes resulting from industrialization and urbanization activities in bursa city. nile tilapias (oreochromis niloticus) were exposed to collected water samples under labor ... | 2010 | 20538035 |
| antibacterial effect (in vitro) of moringa oleifera and annona muricata against gram positive and gram negative bacteria. | antibacterial effects of aqueous and ethanolic extracts of seeds of moringa (moringa oleifera) and pods of soursop (annona muricata) in the concentration of 1:5 and 1:10 in volumes 50, 100, 150 and 200 microl were examined against staphylococcus aureus, vibrio cholerae, escherichia coli (isolated from the organism and the aquatic environment) and salmonella enteritidis. antibacterial activity (inhibition halo > 13 mm) against s. aureus, v. cholerae and e. coli isolated from the whiteleg shrimp, ... | 2010 | 20602021 |
| live recombinant lactococcus lactis vaccine expressing aerolysin genes d1 and d4 for protection against aeromonas hydrophila in tilapia (oreochromis niloticus). | to evaluate a live recombinant lactococcus lactis vaccine expressing aerolysin genes d1 (lac-d1ae) and/or d4 (lac-d4ae) in protection against aeromonas hydrophila in tilapia (oreochromis niloticus). | 2010 | 20602654 |
| analytical approach to the metallomic of nile tilapia (oreochromis niloticus) liver tissue by srxrf and faas after 2d-page separation: preliminary results. | an investigation was made into calcium, iron and zinc in protein spots in samples of nile tilapia (oreochromis niloticus) liver tissue obtained after protein separation by two-dimensional polyacrylamide gel electrophoresis (2d-page) and subsequent qualitative and quantitative evaluation by synchrotron radiation x-ray fluorescence (srxrf) and flame atomic absorption spectrometry (faas). an analysis of the fluorescence spectra indicated the presence of calcium, iron and zinc in 12, 6 and 8 liver p ... | 2010 | 20678667 |
| genotoxic analysis in aquatic environment under influence of cyanobacteria, metal and radioactivity. | the micronucleus (mn) and nuclear abnormality (na) tests were employed to evaluate the genotoxic potential of the lucrecia dam (rn, brazil) located in a semi-arid region and influenced by crop irrigation and irregular rainfall. the analyses of these water samples demonstrated the presence of several cyanobacteria as well as metals and radioactivity. the nile tilapia (oreochromis niloticus) was used for micronucleus and nuclear abnormality assays and analysis of metal concentrations in gills and ... | 2010 | 20691461 |
| the biological role of pituitary adenylate cyclase-activating polypeptide (pacap) in growth and feeding behavior in juvenile fish. | to date, many technologies have been developed to increase efficiency in aquaculture, but very few successful biotechnology molecules have arrived on the market. in this context, marine biotechnology has an opportunity to develop products to improve the output of fish in aquaculture. published in vivo studies on the action of the pituitary adenylate cyclase-activating polypeptide (pacap) in fish are scarce. recently, our group, for the first time, demonstrated the biological role of this neurope ... | 2010 | 20853308 |
| response of antioxidant system of freshwater fish oreochromis niloticus to acute and chronic metal (cd, cu, cr, zn, fe) exposures. | antioxidant systems are known to be sensitive to metal exposures and are suggested to use in predicting sublethal metal toxicity. in this study, several antioxidant enzymes, such as catalase (cat), superoxide dismutase (sod), glutathione s-transferase (gst), glutathione peroxidase (gpx) and glutathione reductase (gr) were measured in the liver and kidney of oreochromis niloticus exposed to sublethal concentrations of metals (cd, cu, cr, zn and fe), using an acute (20 μm, 48 h) or subchronic (10 ... | 2010 | 20870289 |
| [centrocestus formosanus (opisthorchiida: heterophyidae) as a cause of death in gray tilapia fry oreochromis niloticus (perciforme: cichlidae) in the dry pacific of costa rica]. | centrocestusformosanus is a zoonotic trematode from asia and has been mainly associated as cause of death of cultured fish. to identify pathogen trematode species in tilapia fry (oreochromis niloticus) and to determine mollusks hosting these parasites, freshwater mollusks were collected from tilapia cultured ponds and experimental infections were carried out with tilapia fries and different mollusk species. a total of 907 freshwater mollusks were obtained from tilapia ponds and were identified t ... | 2010 | 21250483 |
| streptococcus dysgalactiae as an agent of septicaemia in nile tilapia, oreochromis niloticus (l.). | | 2011 | 21306592 |
| an outbreak of disease caused by francisella sp. in nile tilapia oreochromis niloticus at a recirculation fish farm in the uk. | this study details the first diagnosis of francisella sp. in tilapia in the united kingdom. losses of tilapia fry at a recirculation fish farm in england were investigated, giving a presumptive positive diagnosis of infection with francisella sp. by histopathological examination. most fish sampled showed moderate to marked pathology of the major organs, with lesions being present in most tissues. the most obvious host response was granuloma formulation. a subsequent follow-up visit provided furt ... | 2010 | 21387995 |
| experimental model of microcystin accumulation in the liver of oreochromis niloticus exposed subchronically to a toxic bloom of microcystis sp. | although accumulation of the liver toxin microcystin in phytoplanktivorous fish has been demonstrated in captive fish and in natural ecosystems, the relation between microcystin in ingested algae and the pattern of buildup of microcystin in fish is poorly known. in this month-long study performed at a brazilian fish farm, 45 mature oreochromis niloticus were fed daily with fresh seston periodically dominated by toxic microcystis sp. microcystin was measured daily in the food and every 5 days in ... | 2011 | 21392496 |
| heavy metals in eight edible fish species from two polluted tributaries (aik and palkhu) of the river chenab, pakistan. | concentration of heavy metals (lead (pb), cadmium (cd), chromium (cr), and copper (cu)) was determined in the liver, gills, kidneys, and muscles of eight edible fish species (channa punctata, cirrhinus reba, labeo rohita, heteropneustes fossilis, mystus cavasius, oreochromis niloticus, puntius sophore, and wallago attu) from upstream and downstream zones of the nullah aik and palkhu tributaries of the river chenab located in the sialkot district known for its tanning industries worldwide. the pa ... | 2011 | 21424780 |
| accumulation of microcystins in nile tilapia, oreochromis niloticus l., and effects of a complex cyanobacterial bloom on the dietetic quality of muscles. | the aim of this study was to investigate the kinetics of accumulation and elimination of microcystins in the tissues of nile tilapia (oreochromis niloticus) and to evaluate the effect of cyanobacterial exposure on fish muscle quality (levels of total fat and ash, protein, dry matter and the composition of saturated, monounsaturated and polyunsaturated fatty acids). fish were exposed for 28 days to a natural cyanobacterial bloom with total microcystin concentration around 1,200 μg g(-1) biomass d ... | 2011 | 21533573 |
| modulation of intestinal morphology and immunity in nile tilapia (oreochromis niloticus) by lactobacillus rhamnosus gg. | the use of lactic acid bacteria from human origins as a potential probiotic supplementation in aquaculture feed is now widely accepted. here, we examined some of the properties and mechanisms of the action of lactobacillus rhamnosus gg, originating from humans, on growth performance, gut mucosal immunity and humoral and cellular immune response in tilapia (oreochromis niloticus). the results suggested that supplementation of l. rhamnosus gave an advantage in promoting the intestinal structure an ... | 2011 | 21536310 |
| pathogenicity and oxidative stress in nile tilapia caused by aphanomyces laevis and phoma herbarum isolated from farmed fish. | identified (n = 17) and unidentified (n = 1) fish-pathogenic fungal species from 10 genera of oomycetes and soil fungi were isolated from 40 infected freshwater fish samples of the species oreochromis niloticus niloticus (nile tilapia) and clarias gariepinus (african catfish). samples were collected from various fish farms in the nile delta, egypt. nile tilapia were tested in aquaria for their susceptibility to the commonest oomycetes species, aphanomyces laevis and achlya klebsiana, and also ag ... | 2011 | 21553565 |
| divergence in cis-regulatory sequences surrounding the opsin gene arrays of african cichlid fishes. | abstract: background: divergence within cis-regulatory sequences may contribute to the adaptive evolution of gene expression, but functional alleles in these regions are difficult to identify without abundant genomic resources. among african cichlid fishes, the differential expression of seven opsin genes has produced adaptive differences in visual sensitivity. quantitative genetic analysis suggests that cis-regulatory alleles near the sws2-lws opsins may contribute to this variation. here, we s ... | 2011 | 21554730 |
| immunoproteomic analysis of the antibody response obtained in nile tilapia following vaccination with a streptococcus iniae vaccine. | streptococcus iniae is one of the most economically important gram-positive pathogens in cultured fish species worldwide. the usda-ars aquatic animal health research unit developed a modified (contains concentrated culture supernatant) s. iniae bacterin that has been demonstrated to be efficacious, and protection is mediated by specific anti-s. iniae antibodies. although effective, the specific vaccine components important for efficacy are not known. in the present study, an immunoproteomic appr ... | 2011 | 21601381 |
| identification and expression profile of multiple genes in nile tilapia in response to formalin killed streptococcus iniae vaccination. | twenty-eight expressed sequence tags (ests) were isolated from a nile tilapia (oreochromis niloticus) vaccinated vs non-vaccinated subtractive library at 12-h post injection of a formalin killed streptococcus iniae ars-98-60 vaccine. the 28 ests were classified in terms of their putative functions. half of the ests identified were unknown proteins. of the remaining half ests, 17% have putative functions in protein biosynthesis and 11% have putative functions in immunity, energy production, and s ... | 2011 | 21640389 |
| identification and expression profiles of multiple genes in nile tilapia in response to bacterial infections. | to understand the molecular mechanisms involved in response of nile tilapia (oreochromis niloticus) to bacterial infection, suppression subtractive cdna hybridization technique was used to identify upregulated genes in the posterior kidney of nile tilapia at 6h post infection with aeromonas hydrophila. a total of 31 unique expressed sequence tags (ests) were identified from 192 clones of the subtractive cdna library. quantitative pcr revealed that nine of the 31 ests were significantly (p<0.05) ... | 2011 | 21840065 |
| acute effects of pure cylindrospermopsin on the activity and transcription of antioxidant enzymes in tilapia (oreochromis niloticus) exposed by gavage. | the cyanobacterial toxin cylindrospermopsin (cyn) is a widely distributed contaminant of freshwater systems with the consequent risk for human and wildlife, particularly fish. however, cyn toxicity data on fish are still scarce. it is known that cyn inhibits glutathione synthesis and this could contribute to oxidative damage. in the present work tilapia (oreochromis niloticus) were exposed by gavage to 200 and 400-á++g/kg bw of pure cyn and sacrificed after 24-áh. the activity and relative mrna ... | 2011 | 21695509 |
| the protective role of ascorbic acid (vitamin c) against chlorpyrifos-induced oxidative stress in oreochromis niloticus. | ability of ascorbic acid (vitamin c) to attenuate oxidative damage was evaluated in liver and brain tissues of oreochromis niloticus (o. niloticus) experimentally exposed to sublethal concentrations of chlorpyrifos (cpf). o. niloticus was exposed to sublethal concentrations of cpf at 12-á++g/l (cpf1) and 24-á++g/l (cpf2) for 96-áh. the fish of vitamin c (vit c) and cpf2-á+-ávit c groups were fed with vit c supplemented diet (200-ámg vit c/100-ág feed). a significant increase in thiobarbituric ac ... | 2011 | 21818541 |
| diet supplemented with probiotic for nile tilapia in polyculture system with marine shrimp. | the aim of this study was to assess the effect of a probiotic (lactobacillus plantarum) supplemented diet on nile tilapia (oreochromis niloticus) in a polyculture system with marine shrimp (litopenaeus vannamei) as regards culture performance, hematology, and gut bacterial microbiota. ten 20-m² pens were arranged in one earthen pond and stocked with 2 fish (41.9 g) m(-2) and 10 shrimp (2.3 g) m(-2), in total of 40 nile tilapias and 200 shrimp per experimental unit. tilapia groups in five of the ... | 2011 | 21347844 |
| subchronic effects of cyanobacterial cells on the transcription of antioxidant enzyme genes in tilapia (oreochromis niloticus). | the increasing occurrence of toxic cyanobacterial blooms in eutrophic water bodies is nowadays of worldwide concern due to their ability to produce toxins such as microcystins (mcs). these cyanobacterial toxins have been shown to affect aquatic organisms such as fish, resulting in oxidative stress. among the antioxidant enzymes, glutathione peroxidase (gpx) and soluble glutathione-s-transferases (sgst) play an important role in the detoxification of mcs. in the present work tilapia (oreochromis ... | 2011 | 21279735 |
| francisella asiatica as the causative agent of piscine francisellosis in cultured tilapia (oreochromis sp.) in the united states. | francisella asiatica is a gram-negative, pleomorphic, facultative intracellular, bacterial pathogen that causes acute to chronic disease in a wide variety of warm-water cultured and wild fish species. outbreaks of francisellosis in warm water fish have been documented in taiwan, japan, united kingdom, hawaii, and latin america (including costa rica) but the organism has only been reported from the united states on one occasion from hybrid striped bass in california. in 2010, the bacterium was de ... | 2011 | 21908332 |
| linking fearfulness and coping styles in fish. | consistent individual differences in cognitive appraisal and emotional reactivity, including fearfulness, are important personality traits in humans, non-human mammals, and birds. comparative studies on teleost fishes support the existence of coping styles and behavioral syndromes also in poikilothermic animals. the functionalist approach to emotions hold that emotions have evolved to ensure appropriate behavioral responses to dangerous or rewarding stimuli. little information is however availab ... | 2011 | 22140511 |
| streptococcus agalactiae, an emerging pathogen for cultured ya-fish, schizothorax prenanti, in china. | streptococcus agalactiae (group b streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. s. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. recently, a very serious infectious disease of unknown aetiology broke out in ya-fish (schizothorax prenanti) farms in sichuan province. a gram-positive, chain-forming coccus wa ... | 2011 | 22146014 |
| Fish as a possible reservoir for zoonotic Giardia duodenalis assemblages. | Giardiasis is a re-emerging infectious disease of worldwide significance caused by Giardia duodenalis. This study investigated the occurrence of zoonotic G. duodenalis assemblages in fish to explore the possible role of fish in the epidemiology of human giardiasis. For this purpose, 92 fish (Tilapia nilotica and Mugil cephalus) collected from (fish farms and Nile River) at different governorates in Egypt were examined for the presence of G. duodenalis in their feces by using enzyme linked immuno ... | 2011 | 22160281 |
| laboratory exposure of oreochromis niloticus to crude microcystins (containing microcystin-lr) extracted from egyptian locally isolated strain (microcystis aeruginosa kützing): biological and biochemical studies. | cyanobacterial blooms exert negative impacts on fisheries and water management authorities. recently, it has gained global attention, as elevated earth warming and environmental pollution are accelerating algal growth. oreochromis niloticus (o. niloticus) is a worldwide and the most commonly cultured fish in egypt. the biological interaction of the living organisms to the surrounding environment must continuously be assessed to predict future effects of the ongoing hazards on fish. the study was ... | 2011 | 22095249 |
| Vaccination of sex reversed hybrid tilapia (Oreochromis niloticus × O. aureus) with an inactivated Vibrio vulnificus vaccine. | Vibrio vulnificus causes disease in economically important aquaculture raised fish and is an opportunistic human pathogen. This study reports on the isolation of V. vulnificus from diseased hybrid tilapia (Oreochromis niloticus × O. aureus) cultured in a North American water reuse facility. Our objectives were to characterize the isolate using biochemical and molecular methods, develop a disease challenge model, and determine the ability of a formalin inactivated whole-cell vaccine to protect ag ... | 2011 | 21982852 |
| autohydrolysed tilapia nilotica fish viscera as a peptone source in bacteriocin production. | fish processing generates large amounts of solid and liquid wastes. many different by-products have been produced from fish processing wastes. studies on solubilization of bolti fish (tilapia nilotica) viscera by endogenous enzymes at different phs are described. hydrolysis reactions were conducted with freshly thawed viscera utilizing an initial temperature gradient and terminated at various time points by heat inactivation of the enzymes. various peptones obtained from hydrolysed visceral homo ... | 2011 | 22654160 |
| gut microbial status induced by antibiotic growth promoter alters the prebiotic effects of dietary dvaqua® on aeromonas hydrophila-infected tilapia: production, intestinal bacterial community and non-specific immunity. | the purpose of the present study was to investigate whether dietary antibiotic-induced changes in the fish intestinal microbiota altered host physiological responses to the infection with aeromonas hydrophila in hybrid tilapia (oreochromis niloticus ♀ × o. aureus ♂). after an 8-week induction period with an antibiotic-supplemented or antibiotic-non-supplemented diet, 160 hybrid tilapias in 16 tanks were each injected with phosphate buffered saline (pbs) or a. hydrophila at a dose of one-half of ... | 2010 | 21146333 |
| oxidative stress in nile tilapia (oreochromis niloticus) and armored catfish (pterygoplichthys anisitsi) exposed to diesel oil. | considering that diesel oil is one of the most common aquatic contaminants, we compare the oxidative stress between two species of fish with different habitats (pterygoplichthys anisitsi, benthic and oreochromis niloticus, nektonic) exposed to diesel oil. malondialdehyde concentrations (mda) and the activities of ethoxyresorufin-o-deethylase (erod), glutathione s-transferase (gst), catalase (cat), superoxide dismutase (sod), and glutathione peroxidase were all analyzed in the fishes' livers and ... | 2010 | 21108001 |
| impact of microcystin containing diets on physiological performance of nile tilapia (oreochromis niloticus) concerning detoxification. | nile tilapia (oreochromis niloticus) were fed by diets supplemented with cyanobacteria containing in part the cyanotoxin microcystin-lr (mc-lr) to determine the potential impacts on detoxification. four different diets were prepared based on a commercial diet: (1) control, (2) mc-5% (containing 5% dried microcystis sp. biomass with 4.92 μg mc-lr g(-1) diet), (3) mc-20% (containing 20% dried microcystis sp. biomass with 19.54 μg mc-lr g(-1) diet), and (4) arthr-20% (containing 20% dried arthrospi ... | 2010 | 20949194 |
| the food of the larvivorous fish gambusia affinis (baird and girard) and oreochromis (formerly tilapia) niloticus (linnaeus) in gezira irrigation canals. | the food of both gambusia affinis and oreochromis niloticus was studied. organisms eaten by both species of fish are tabulated, together with the amounts eaten during the various months of the year. g. affinis larger than 25 mm are carnivorous and become more so with age. food selection by g. affinis depends on the availability of food items rather than choice. it showed a marked preference for mosquito larvae. o. niloticus smaller than 150 mm were markedly carnivorous, but this decreased with a ... | 2010 | 2863390 |
| oxidative stress response in nile tilapia (oreochromis niloticus) exposed to textile mill effluent. | textile mill effluent is an important pollution factor because of its organic and inorganic substances content. in this study, toxic effects of textile mill effluent on fish oreochromis niloticus were investigated by using antioxidant enzymes and lipid peroxidation (lpo) responses. superoxide dismutase (sod), catalase (cat) enzyme activities and lipid peroxidation by means of thiobarbituric reactive substances (tbars) level were measured in liver and gill tissues of fish. the fish were exposed t ... | 2011 | 20858649 |
| metabolism of three stereoisomers of astaxanthin in the fish, rainbow trout and tilapia. | in rainbow trout (salmo gairdneri), the dietary astaxanthin diesters were mostly absorbed and accumulated in their integuments keeping their configurations, and partially metabolized to (3r, 3'r)-zeaxanthin (major) and/or (3r, 3's)-zeaxanthin (medium) and/or (3s,3's)-zeaxanthin (minor). in tilapia (tilapia nilotica), the three stereoisomers of astaxanthin diesters were promptly metabolized to only (3s,3's)-astaxanthin, and subsequently to (3r,3'r)-zeaxanthin and/or (3r,3's)-zeaxanthin and/or (3s ... | 2013 | 3829622 |
| identification and pathogenicity of aeromonas sobria on tail-rot disease in juvenile tilapia oreochromis niloticus. | thirty-six strains, numbered from py01 to py36, were isolated from six moribund oreochromis niloticus. the biochemical characteristics of all strains conformed to the species description of aeromonas sobria on the basis of api 20e and biolog gn system. furthermore, gyrb sequence of strain py36 was sequenced and showed high similarity (99.8%) with a. sobria in genbank. antibiotic-resistance of strain py36 was assessed by the kirby-bauer disk diffusion method, and the results showed it was suscept ... | 2011 | 20853167 |
| effects of dietary antibiotic growth promoter and saccharomyces cerevisiae fermentation product on production, intestinal bacterial community, and nonspecific immunity of hybrid tilapia (oreochromis niloticus female x oreochromis aureus male). | to investigate the effects of a dietary antibiotic growth promoter (florfenicol) and a saccharomyces cerevisiae fermentation product (dvaqua) on growth, g:f, daily feed intake, intestinal bacterial community, and nonspecific immunity of hybrid tilapia (oreochromis niloticus ♀ × oreochromis aureus ♂), a 16-wk feeding trial was conducted in a recirculating aquaculture system. four feeding regimens were evaluated: control, dietary florenicol (0.02 g/kg; 16 wk), dietary dvaqua (0.5 g/kg; 16 wk), and ... | 2011 | 20852079 |
| comparative sequence analysis of a multidrug-resistant plasmid from aeromonas hydrophila. | aeromonas hydrophila is a pathogenic bacterium that has been implicated in fish, animal, and human disease. recently, a multidrug resistance (mdr) plasmid, pr148, was isolated from a. hydrophila obtained from a tilapia (oreochromis niloticus) farm in thailand. pr148 is a 165,906-bp circular plasmid containing 147 coding regions showing highest similarity to pndm-1_dok1, an mdr plasmid isolated from a human pathogen. pr148 was also very similar to other inca/c plasmids isolated from humans, anima ... | 2013 | 23070174 |
| myo-inositol phosphate synthase expression in the european eel (anguilla anguilla) and nile tilapia (oreochromis niloticus): effect of seawater acclimation. | a single mips gene (isyna1/ino1) exists in eel and tilapia genomes with a single myo-d-inositol 3-phosphate synthase (mips) transcript identified in all eel tissues, although two mips spliced variants [termed mips(s) and mips(l)] are found in all tilapia tissues. the larger tilapia transcript [mips(l)] results from the inclusion of the 87-nucleotide intron between exons 5 and 6 in the genomic sequence. in most tilapia tissues, the mips(s) transcript exhibits much higher abundance (generally >10- ... | 2016 | 27252471 |
| identification and characterization of micrornas in ovary and testis of nile tilapia (oreochromis niloticus) by using solexa sequencing technology. | micrornas (mirnas) are endogenous non-coding small rnas which play important roles in the regulation of gene expression by cleaving or inhibiting the translation of target gene transcripts. thereinto, some specific mirnas show regulatory activities in gonad development via translational control. in order to further understand the role of mirna-mediated posttranscriptional regulation in nile tilapia (oreochromis niloticus) ovary and testis, two small rna libraries of nile tilapia were sequenced b ... | 2014 | 24466258 |
| neurokinin b-related peptide suppresses the expression of gnrh i, kiss2 and tac3 in the brain of mature female nile tilapia oreochromis niloticus. | neurokinin b (nkb) and neurokinin b related peptide (nkbrp) belong to tachykinin peptide family. theyact as a neurotransmitter and/or neuromodulator. mutation of nkb and/or its cognate receptor, nk3r resulted in hypogonadotropic hypogonadism in mammals, implying a strong involvement of nkb/nk3r system in controlling mammalian reproduction. teleosts possess nkbrp as well as nkb, but their roles in fish reproduction need to be clarified. in this study, nkb and nkbrp coding gene (tac3) was cloned f ... | 0 | 27294210 |
| integrating cytogenetics and genomics in comparative evolutionary studies of cichlid fish. | the availability of a large number of recently sequenced vertebrate genomes opens new avenues to integrate cytogenetics and genomics in comparative and evolutionary studies. cytogenetic mapping can offer alternative means to identify conserved synteny shared by distinct genomes and also to define genome regions that are still not fine characterized even after wide-ranging nucleotide sequence efforts. an efficient way to perform comparative cytogenetic mapping is based on bac clones mapping by fl ... | 2012 | 22958299 |
| effects of the antibiotic growth promoters flavomycin and florfenicol on the autochthonous intestinal microbiota of hybrid tilapia (oreochromis niloticus ♀ × o. aureus ♂). | the 16s rdna pcr-dgge and rpob quantitative pcr (rq-pcr) techniques were used to evaluate the effects of dietary flavomycin and florfenicol on the autochthonous intestinal microbiota of hybrid tilapia. the fish were fed four diets: control, dietary flavomycin, florfenicol and their combination. after 8 weeks of feeding, 6 fish from each cage were randomly chosen for the analysis. the total number of intestinal bacteria was determined by rq-pcr. the results showed that dietary antibiotics signifi ... | 2010 | 20844867 |
| development of a pcr assay based on a single-base pair substitution for the detection of aeromonas caviae by targeting the gyrb gene. | aeromonas caviae is a bacterial pathogen that causes various infectious diseases in both humans and animals. to facilitate its detection, we developed species-specific primer sets targeting polymorphisms in the gyrb gene for use in a pcr assay. the technique was able to detect 100% (29/29) of the a. caviae strains tested using either of two sets of primers (designated acf1-acr and acf3-acr), which produced 293-bp and 206-bp amplicons, respectively. another set of primers (designated acf2-acr) yi ... | 2015 | 26223267 |
| identification and virulence of aeromonas dhakensis, pseudomonas mosselii and microbacterium paraoxydans isolated from nile tilapia, oreochromis niloticus, cultivated in mexico. | to identify bacterial pathogens of diseased niie tilapia oreochromis niloticus and determine their virulence. | 2013 | 23758410 |
| impact of microcystin containing diets on physiological performance of nile tilapia (oreochromis niloticus) concerning stress and growth. | diets containing microcystis with considerable amounts of the cyanotoxin microcystin-lr (mc-lr) were fed to determine their impact on the physiological performance of the omnivorous nile tilapia (oreochromis niloticus) with regard to stress and growth performance. four different diets were prepared based on a commercial diet (control, mc-5% [containing 5% dried microcystis biomass], mc-20% [containing 20% dried microcystis biomass], and arthrospira-20% [containing 20% dried arthrospira sp. bioma ... | 2010 | 20821479 |
| use of asiatic pennywort centella asiatica aqueous extract as a bath treatment to control columnaris in nile tilapia. | to develop antibiotic-free and chemical-free aquaculture, it is necessary to have natural substances to control diseases of aquatic animals. the aim of this study was to find an herb having therapeutic effect against columnaris, a fish disease caused by the bacterium flavobacterium columnare. of all tested herbs (including kalmegh andrographis paniculata, candle bush cassia alata, asiatic pennywort centella asiatica, mangosteen garcinia mangostana, pomegranate punica granatum, and guava psidium ... | 2010 | 20575361 |