| recent vesicular stomatitis virus infection detected by immunoglobulin m antibody capture enzyme-linked immunosorbent assay. | we developed an enzyme-linked immunosorbent assay (elisa) that was capable of detecting immunoglobulin m (igm) antibody to vesicular stomatitis virus (vsv) in the sera of experimentally and naturally infected cattle and horses. the detection of igm in the sera of these animals permitted an estimate of the recency of infection by vsv serotype new jersey. a vsv serotype new jersey epizootic strain isolated from a horse and passed once in an aedes albopictus cell line was used to infect a horse and ... | 1985 | 3001132 |
| site of suppression of banzi viral replication by an antiviral factor released from aedes albopictus cells persistently infected with banzi virus. | the ability of the antiviral factor present in culture medium of aedes albopictus cells persistently infected with the flavivirus, banzi virus, to inhibit the replication of banzi virus was examined. the anti-banzi viral factor did not inhibit the uncoating of the virion. levels of viral rna were markedly reduced in mosquito cells treated with the antiviral factor. syntheses of negative-strand and of positive-strand viral rna species were inhibited to approximately the same extent. this inhibiti ... | 1985 | 3002069 |
| study of receptors for vesicular stomatitis virus in vertebrate and invertebrate cells. | early interactions between vesicular stomatitis virus (vsv) and susceptible cells were examined in cell lines of mammalian (hela), bird (cer), piscine (epc) and arthropod (aedes albopictus) origin showing different permissiveness to vsv growth. the chemical nature of receptors was investigated either by modification of cell surfaces with different enzymes or by competition for vsv binding between extracted membrane components and whole cells. results obtained indicate that in all cell models, me ... | 1986 | 3018449 |
| susceptibility of indonesia colonies of aedes aegypti and aedes albopictus mosquitoes to experimental infection with chikungunya virus. | | 1986 | 3023740 |
| bacillus thuringiensis var. israelensis delta-endotoxin. cloning and expression of the toxin in sporogenic and asporogenic strains of bacillus subtilis. | a plasmid-borne gene from bacillus thuringiensis var. israelensis encoding a 27,340 mr insecticidal delta-endotoxin has been cloned on a bifunctional multicopy plasmid in a wild-type sporogenic strain and two asporogenic mutants of bacillus subtilis. the delta-endotoxin gene is expressed at a low level during vegetative growth in all three strains, but the synthesis of the toxin increases markedly during the third hour of stationary phase for both the sporogenic strain and an asporogenic mutant ... | 1986 | 3025453 |
| entry pathway of vesicular stomatitis virus into different host cells. | a biochemical and morphological investigation of the mechanism of entry of vesicular stomatitis virus (vsv) into host cells of mammalian (hela), avian (cer), piscine (epc) and arthropod (aedes albopictus) origin, is described. vsv was capable of infecting all cell lines tested by a endosome- and/or a lysosome-dependent step since ammonium chloride and amantadine blocked the early stages of infection. complement-dependent immune lysis of infected host cells provided evidence that in none of the f ... | 1987 | 3029282 |
| ultrastructural studies of kunjin virus-infected aedes albopictus cells. | ultrastructural changes in aedes albopictus cells infected with kunjin virus were characterized from 12 to 72 h post-infection. early in infection (16h), there were no prominent ultrastructural changes except for an increase in the number of vacuoles in the cytoplasm. as the infection progressed the rough endoplasmic reticulum appeared to lengthen and whorls of fibres were observed within some vacuoles. virus particles were observed in small numbers scattered in the cytoplasm between 24 to 30 h ... | 1987 | 3029292 |
| sindbis virus mutants resistant to mycophenolic acid and ribavirin. | previous work from this laboratory has demonstrated a correlation between the inhibition by ribavirin (rbv), mycophenolic acid (mpa), or 2-amino thiadiazole (tda) of sindbis virus replication in aedes albopictus mosquito cells and a reduction in cellular gtp levels. this reduction in gtp results from the inhibition by these drugs of inosine monophosphate dehydrogenase (impdh), the first enzyme specific for the de novo synthesis of gmp. by serial passage of sv in a. albopictus cells in the presen ... | 1987 | 3033882 |
| [pathogenicity of spiroplasma sabaudiense (mollicute) for the cells (c6/36) of aedes albopictus (insecta: diptera) in vitro]. | an initial stock of spiroplasma sabaudiense had been maintained in cell-free medium for 2-3 years. subsequent passages in leibovitz's medium l-15 in the presence of aedes albopictus (c6/36) cells dramatically increased the pathogenicity of s. sabaudiense towards these cells. cytopathogenicity included production of syncytia, an increase in the number of mitochondria and alteration of their morphology, vacuolisation and a reduction in the rate of growth. cell lysis was associated with cytadsorpti ... | 1988 | 3064943 |
| aedes albopictus infestation--united states, brazil. | | 1986 | 3090418 |
| aedes albopictus introduction into continental africa, 1991. | from april 15 through july 20, 1991, an epidemic of yellow fever (yf) occurred in delta state, nigeria. in september 1991, as part of a follow-up investigation, mosquito oviposition cups were deployed in four rural communities with yf, all within a 24-kilometer radius of the principal town of agbor. based on findings from the follow-up investigation, this report documents the first record of breeding populations of ae. albopictus--a competent yf virus vector--in continental africa. | 1991 | 1956370 |
| the surface charge of l-a9 cells and aedes albopictus cells infected with marituba (bunyaviridae) virus. | the surface charge of marituba virus infected l-a9 cells and aedes albopictus cells was estimated by direct measurement of their electrophoretic mobilities. uninfected l-a9 cells and a. albopictus cells have mean electrophoretic mobilities of -1.083 microns/s x cm/v and -1.019 microns/s x cm/v, respectively. in marituba virus infected l-a9 cells a progressive decline in the electrophoretic mobility was observed. in contrast, in marituba virus infected a. albopictus cells the electrophoretic mobi ... | 1987 | 3111119 |
| geographic origin of the us and brazilian aedes albopictus inferred from allozyme analysis. | a survey of genetic variation using allozymes was conducted on 57 populations of aedes albopictus, an asian mosquito that was introduced into the us and brazil in the mid-1980s. our objective was to quantify the patterns of genetic variation among its populations and to use that information to trace genetically the geographic origin of the us and brazilian populations. populations from the various regions were genetically distinct from one another. populations from within a region were genetical ... | 1991 | 1917554 |
| an orbivirus of mosquitoes which induces co2 sensitivity in mosquitoes and is lethal for rabbits. | an orbivirus, jkt-7400, isolated from culex mosquitoes in indonesia, replicated to a high titer and induced cytopathic effects in aedes albopictus cell cultures. the virus produced lethal sensitivity to carbon dioxide in culex and aedes mosquitoes as well as in drosophila melanogaster fruit flies but was not the agent of the hereditary sensitivity to carbon dioxide previously described for culex quinquefasciatus. when injected intravenously in high doses, jkt-7400 virus was lethal for rabbits, a ... | 1988 | 3136255 |
| vector competence of a houston, texas strain of aedes albopictus for rift valley fever virus. | | 1988 | 3193106 |
| a survey of mosquitoes breeding in used tire stockpiles in connecticut. | a larval survey of nine used tire disposal sites in connecticut, was conducted from june through october 1987. no larvae of aedes albopictus were uncovered indicating that this mosquito is not yet established in the state. however, eight other mosquito species in four genera were found. the most abundant and widely distributed species found breeding in permanent tire dumps were ae. atropalpus, ae. triseriatus and culex restuans. culex pipiens was the second most common culex species followed by ... | 1988 | 3199116 |
| effects of dengue-1 infection in aedes albopictus on its susceptibility to malathion. | | 1988 | 3199129 |
| arboviruses recovered from sentinel cattle using several virus isolation methods. | a group of 20 sentinel steers was bled weekly for 5 months in 1986 and the blood samples were examined for arboviruses by inoculation firstly into embryonated chicken eggs (ece), baby mice, aedes albopictus cells and bhk21 monolayers. a second group of cattle was similarly examined for virus in 1987, except that baby mice were not used. viruses were recovered from 26% of the 878 weekly bleeds. the viruses identified consisted of 14 types belonging to the bluetongue, epizootic haemorrhagic diseas ... | 1988 | 3218074 |
| survey of adult mosquitoes (diptera: culicidae) during irrigation development in the mahaweli project, sri lanka. | a survey of adult mosquitoes was done during 1986-1987 in an area undergoing irrigation development in the mahaweli project of sri lanka. the study encompassed two 12-mo phases of settlement and irrigation, respectively. diurnal human bait catches yielded 1,427 female mosquitoes in 27 species. nocturnal collections (human bait, cdc light traps, indoor resting catches) produced a total of 30,491 females in 67 species. comparisons with an earlier survey in the predevelopment forest showed that the ... | 1991 | 1678784 |
| [variations in the phenotypic expression of the western nile fever virus after reproduction in two aedes albopictus skuse, 1895 cell lines]. | | 1988 | 3231175 |
| aedes albopictus mosquitoes introduced into brazil: vector competence for yellow fever and dengue viruses. | | 1988 | 3232189 |
| [arbovirus infections on the island of nosy-be; serologic and entomologic findings]. | since 1977, the pasteur institute of madagascar has been studying, during six surveys, the arboviruses of nosy-be area, in the north-west of madagascar. 47.2 p. 100 out of 271 human sera and 11.3 p. 100 out of 150 animal sera (mostly from lemurs), tested for antibodies to 16 arboviruses by the haemagglutination inhibition test, are positive. the results show an important prevalence of flaviviruses. west-nile and dengue 1 viruses were probably circulating some years before the surveys. antibodies ... | 1988 | 3242421 |
| detection of dengue virus type 2 in aedes albopictus by nucleic acid hybridization with strand-specific rna probes. | a molecular hybridization technique with radiolabeled, strand-specific rna probes was developed to detect dengue virus type 2 rna in pools of infected aedes albopictus mosquitoes. one infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infectious doses. | 1988 | 3281980 |
| cytolytic activity and immunological similarity of the bacillus thuringiensis subsp. israelensis and bacillus thuringiensis subsp. morrisoni isolate pg-14 toxins. | the parasporal bodies of the mosquitocidal isolates of bacillus thuringiensis subsp. israelensis and b. thuringiensis subsp. morrisoni isolate pg-14 were compared with regard to their hemolytic and cytolytic activities and the immunological relatedness of the 28- and 65-kilodalton (kda) proteins that occur in both subspecies. the alkali-solubilized parasporal bodies of b. thuringiensis subsp. israelensis caused 50% lysis of human erythrocytes at 1.14 micrograms/ml, whereas those of b. thuringien ... | 1987 | 3300548 |
| dengue haemorrhagic fever in malaysia: a review. | the historical background, epidemiology and changing pattern of clinical disease as seen in malaysia is reviewed. the preliminary results of the longitudinal study of epidemiology of dengue in malaysia is also presented. studies led by rudnick et al. over some 18 years have established that the disease is endemically transmitted by both aedes aegypti and aedes albopictus causing illnesses ranging from mild febrile episodes through classical dengue fever, dengue haemorrhagic fever and the dengue ... | 1987 | 3324361 |
| characterization of the toxicity and cytopathic specificity of a cloned bacillus thuringiensis crystal protein using insect cell culture. | an insecticidal protein gene from bacillus thuringiensis var. aizawai was cloned in escherichia coli. the cloned gene expressed at a high level and the synthesized protein appeared as an insoluble, phase-bright inclusion in the cytoplasm. these inclusions were isolated by density gradient centrifugation, the isolated protein was activated in vitro by different proteolytic regimes and the toxicity of the resulting preparations was studied using insect cells grown in tissue culture. the inclusions ... | 1987 | 3330756 |
| genetics of aedes albopictus. | aedes albopictus is an important vector of dengue fever and dengue hemorrhagic fever in southeast asia. its distribution extends from madagascar to hawaii and is currently expanding. from its proposed origin in southeast asia, ae. albopictus has migrated as far as mauritius and madagascar to the west and korea, japan, guam, hawaii and other pacific islands to the east. in the continental united states, it was originally reported in the texas area in august 1985 and is now well established in sev ... | 1986 | 3333792 |
| leads from the mmwr. update: aedes albopictus infestation--united states. | | 1988 | 3336132 |
| svlm21, a mutant of sindbis virus able to grow in aedes albopictus cells in the absence of methionine, shows increased sensitivity to s-adenosylhomocysteine hydrolase inhibitors such as neplanocin a. | inhibition of s-adenosylhomocysteine (adohcy) hydrolase by compounds such as neplanocin a (npa) leads to the build-up of adohcy and the inhibition of methyltransferase enzymes. whether assayed by efficiency of plaquing or virus yield, svlm21, a mutant of sindbis virus resistant to methionine deprivation, was more sensitive to npa than was the standard virus (svstd) from which it was derived. for example, whereas 10 micrograms npa/ml depressed the yield of svlm21 by more than 30-fold, the yield o ... | 1988 | 3347999 |
| exposure of semliki forest virus-infected baby hamster kidney cells to low ph leads to a proton influx and a rapid depletion of intracellular atp which in turn prevents cell-cell fusion. brief report. | the conditions needed to trigger semliki forest virus (sfv) induced cell-cell fusion from within (ffwi) of baby hamster kidney (bhk) cells differs from some other cells (e.g., aedes albopictus cells). in contrast to such cells it is mandatory for bhk cells to be brought back to a neutral ph after a short exposure to mildly acidic ph for fusion to occur. this phenomenon can be explained by a sudden drop of the intracellular ph observed after clamping the extracellular ph below 6.2--the ph require ... | 1988 | 3355372 |
| mitochondrial dna variation within and among populations of the mosquito aedes albopictus. | a survey of restriction fragment polymorphism in mitochondrial dna (mtdna) of 17 populations of the mosquito aedes albopictus was undertaken. the mtdna size was estimated to be about 17.5 kbp. the level of polymorphism was low, with over 99% of the fragments being shared in common among the 17 populations. three populations, mauritius, singapore, and hong kong, contained individuals with both the ancestral and novel mtdna haplotypes. we conclude that the low level of mtdna polymorphism in a. alb ... | 1991 | 1676010 |
| effect of actinomycin d and cycloheximide on replication of sindbis virus in aedes albopictus (mosquito) cells. | production of sindbis virus in the presence of transcription and translation inhibitors was examined in three aedes albopictus cell lines. addition of cycloheximide to heat-resistant sindbis virus (svhr)-infected mosquito cells arrested viral rna synthesis completely, in contrast to the effects of this drug on virus-infected vertebrate cells. production of mature virus by both svhr (a variant commonly used as a wild-type virus) and sbamr (a mutant which is resistant to the effects of 18 h of pre ... | 1988 | 3392770 |
| survival of albumin, igg, igm, and complement (c3) in human blood after ingestion by aedes albopictus and phlebotomus papatasi. | the levels of albumin, igg, igm, and complement (c3) were determined by enzyme-linked immunosorbent assay in aedes albopictus and phlebotomus papatasi at regular intervals after feeding on human blood. albumin disappeared most rapidly; by 48 hr, detectable levels of albumin had decreased 100-fold. in contrast, igg and igm survived longer and were still detectable at low levels several days after ingestion. c3 was intermediate in its rate of degradation. in general, serum protein disappearance oc ... | 1988 | 3400799 |
| factors affecting polybrene-mediated transfection of cultured aedes albopictus (mosquito) cells. | the polycation 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) is superior to calcium phosphate for the introduction of purified dna into cultured aedes albopictus (mosquito) cells. adsorption of the polybrene-dna complex to mosquito cells was essentially linear for 6 h. however, the rate of adsorption of dna increased when the dna-polybrene mixture was preincubated for several hours prior to addition to cells. a recombinant plasmid carrying an inducible chloramphenicol acetyl ... | 1986 | 3462005 |
| variation in the vector competence of geographic strains of aedes albopictus for dengue 1 virus. | eight geographic strains of aedes albopictus from asia and north america and one north american strain of aedes aegypti were tested for their vector competence with dengue 1 virus. three groups of ae. albopictus were established based on their vector competence: a) the oahu laboratory strain, b) the three malaysian strains, and c) the tokyo and three north american strains. the three north american strains were similar to the strain of ae. aegypti from houston, texas in their ability to transmit ... | 1987 | 3504922 |
| the perspective of aedes albopictus from the administrative viewpoint. | mosquito control administrators should consider a newly introduced species in view of local conditions. aedes albopictus was discovered in harris county while surveying for aedes aegypti. recognizing a potential threat to public health, local and state officials were notified of the discovery. questions posed by the introduction of a foreign species requires a cooperative application of research resources. the centers for disease control (cdc) should serve as the coordinator of this effort. | 1986 | 3507521 |
| fusion of semliki forest virus infected aedes albopictus cells at low ph is a fusion from within. | herein, it is shown for the first time that the mechanism of fusion followed in aedes albopictus cells infected with semliki forest virus induced by low ph exposure is a "fusion from within". several parameters were studied disclosing that the development of the fusion capacity of the cells is directly related to the synthesis of viral specific products. these findings were further substantiated by utilizing various chemicals to inhibit viral specific events during infection, protein synthesis a ... | 1986 | 3521544 |
| use of the avidin-biotin-peroxidase complex immunocytochemical procedure for detection of rift valley fever virus in paraffin sections of mosquitoes. | the avidin-biotin-peroxidase complex (abc) immunocytochemical procedure was used to locate rift valley fever (rvf) virus antigen in infected aedes albopictus c6/36 cultured cells and in serial paraffin sections of intrathoracically-injected egyptian culex pipiens. fixation of the cultured cells in formaldehyde or periodate-lysine-paraformaldehyde, and fixation of whole mosquitoes with formaldehyde resulted in good preservation of morphology and excellent differential staining between uninfected ... | 1986 | 3532843 |
| isolation of a new rhabdovirus in australia related to tibrogargan virus. | a virus isolated from the blood of a healthy steer and designated dpp53 was shown to have rhabdovirus morphology. although dpp53 virus was antigenically related to tibrogargan virus by reciprocal immunofluorescence and neutralization tests, the viruses were distinguishable by neutralization tests. dpp53 virus contained rna and was sensitive to both ether and chloroform. the geographical distribution of neutralizing antibody to dpp53 virus in australian cattle corresponded to the distribution of ... | 1986 | 3548679 |
| aedes albopictus in north america: probable introduction in used tires from northern asia. | north american strains of aedes albopictus, an asian mosquito recently introduced into the western hemisphere, exhibit photoperiodic sensitivity and cold-hardiness characteristics similar to strains originating from temperate zone asia. trade statistics for used tire imports, the most likely mode of introduction, also indicate a north asian origin. aedes albopictus, an important vector of dengue and a potential vector of many other arboviral diseases, may therefore have the capability of infesti ... | 1987 | 3576225 |
| a study on susceptibility of indonesia colonies of aedes aegypti and aedes albopictus mosquitoes to experimental infection with dengue type 3 and chikungunya viruses. | | 1987 | 3586577 |
| an outbreak of dengue virus at rio de janeiro--1986. | dengue virus type 1 has been isolated in aedes albopictus cell strain, from sera of patients living in the nova iguaçu county, by rio de janeiro. the clinical picture was characterized by fever, headache, retrobulbar pain, backache, pains in the muscles and the joints and prostration. studies in paired sera confirmed the presence of recent infection by dengue virus type 1. the outbreak reached adjacent areas, including rio de janeiro city (may, 1986). | 1986 | 3587006 |
| semliki forest virus-induced polykaryocyte formation is an atp-dependent event. | infection of aedes albopictus cells with semliki forest virus (sfv) leads to polykaryocyte formation below ph 6.2. this syncytium formation is accompanied by a decrease of the cellular atp level. addition of inhibitors of oxidative phosphorylation leads to a rapid, total depletion of atp in infected cells at ph 6 and results in an inhibition of polykaryocyte formation. however, when cells were exposed for only a few minutes to ph 6 in the presence of the inhibitors and then kept at ph 7.2, the a ... | 1987 | 3592981 |
| multiplication of chikungunya virus in salivary glands of aedes albopictus (oahu strain) mosquitoes: an electron microscopic study. | aedes albopictus as well as aedes aegypti is an important vector of chikungunya and dengue viruses. electron microscopic observations on the salivary glands of ae. albopictus infected with chikungunya virus were performed in comparing with those of ae. aegypti infected with dengue virus. no virus budding from the cell surface of the chikungunya-infected mosquito's salivary glands was found as shown in dengue-infected ones, in contrast to the findings of the mammalian cells such as vero, kb, imr, ... | 1986 | 3599528 |
| a novel method for the detection of early events in cell-cell fusion of semliki forest virus infected cells growing in monolayer cultures. | semliki forest virus infected aedes albopictus cells were used to investigate virus induced cell-cell fusion. it was shown by a novel method that cell-cell fusion was completed within approximately 5 minutes after triggering the fusion event by low ph. this method consists of fixing fusing cells with glutaraldehyde and microinjecting the highly fluorescent and rapidly diffusing dye lucifer yellow. in contrast, polykaryon formation, the usually used criterion to measure cell-cell fusion occurred ... | 1987 | 3606395 |
| susceptibility of aedes albopictus c6/36 cells to viral infection. | the susceptibility of the c6/36 clone of aedes albopictus monolayer cell cultures was determined with 46 prototype viruses passed through three subcultures. viral growth was confirmed by titration of the passage material in other susceptible host systems. nineteen viruses demonstrated good growth in c6/36 cells: coxsackievirus group a type 10 and group b types 2, 3, 4, and 5; enterovirus 69; mumps virus; poliovirus types 1 to 3; reovirus types 1 to 3; vaccinia virus; dengue virus type 2; eastern ... | 1987 | 3611315 |
| sexual transmission of dengue viruses by aedes albopictus. | male aedes albopictus experimentally infected with dengue virus types 1, 2, 3, or 4 transmitted their infection sexually to female ae. albopictus. such transmission was enhanced if the females had taken a bloodmeal 2 to 7 days prior to mating. male ae. albopictus also transmitted dengue virus vertically to their f1 progeny. infected progeny were found among those derived from eggs laid greater than or equal to 73 hr after mating but not among those derived from eggs laid prior to that time. this ... | 1987 | 3661831 |
| in vitro cultivation of rickettsia-like-organisms from glossina spp. | a method is described for the in vitro cultivation of the rickettsia-like-organisms (rlo) from glossina spp. which are believed to be associated with susceptibility to trypanosome infection. cultures of rlo were established by infecting a mosquito cell line (aedes albopictus) with haemolymph taken from teneral flies. rlo from nine species of glossina have been isolated and maintained in continuous culture using this technique. | 1987 | 3662675 |
| effect of hypertonic medium on the protein synthesis in l-a9 and aedes albopictus cells infected with mayaro virus. | | 1991 | 1668789 |
| stable transformation of a mosquito cell line results in extraordinarily high copy numbers of the plasmid. | stable incorporation of high copy numbers (greater than 10,000 per cell) of a plasmid vector containing a gene conferring resistance to the antibiotic hygromycin was achieved in a cell line derived from the aedes albopictus mosquito. plasmid sequences were readily observed by ethidium bromide staining of cellular dna after restriction endonuclease digestion and agarose gel electrophoresis. the plasmid was demonstrated by in situ hybridization to be present in large arrays integrated in metaphase ... | 1992 | 1631052 |
| sequence analysis of the e2 gene of a hyperglycosylated, host restricted mutant of sindbis virus and estimation of mutation rate from frequency of revertants. | svap15/21, a strain of sindbis virus (sv) derived from our standard laboratory strain of sv (svstd) after repeated passage on aedes albopictus cells, grows normally in mosquito cells but is host restricted (hr) in vertebrate cells. it is also temperature sensitive (ts) and produces pinpoint plaques on vertebrate cells (sp). e2 glycoprotein of svstd differs from that of the more widely used svhr (from which svstd was derived) by an additional (i.e., third) n-linked glycan. the e2 of svap15/21, in ... | 1986 | 3750843 |
| dominance of the cpe(+) phenotype in hybrid aedes albopictus cells infected with sindbis virus. | the effect of sindbis virus (sv) infection was analyzed in hybrid aedes albopictus cells formed by fusing ouabain-resistant cpe(+) cells to cpe(-) alpha-amanitin resistant cells. although the 24-h yields of virus from the parental cpe(+) and cpe(-) clones were similar, the rates of viral rna synthesis and virus release at early times post-infection were higher in the cpe(+) cells. in all eight hybrid clones studied, the cpe(+) phenotype was dominant. in addition, the kinetics of viral rna synthe ... | 1986 | 3765819 |
| aedes albopictus, yellow fever, and the americas. | | 1987 | 3800201 |
| detection of chikungunya virus antigen in aedes albopictus mosquitoes by enzyme-linked immunosorbent assay. | double-antibody sandwich and modified sandwich systems of enzyme-linked immunosorbent assay for detecting chikungunya virus antigen present in female mosquitoes, aedes albopictus (oahu strain), were evaluated as simple and rapid methods of selection of a highly susceptible mosquito line. both assays were capable of detecting 3.9 x 10(1) ng (4.0 x 10(6) pfu) or more of the purified antigen. an inhibition system was less sensitive, and a direct system with adsorption of test specimens on the solid ... | 1985 | 3833871 |
| aedes albopictus introduction into continental africa, 1991. | | 1992 | 1567751 |
| titer distribution analysis of chikungunya virus in aedes albopictus (diptera: culicidae). | | 1986 | 3950931 |
| exclusion of superinfecting homologous virus by sindbis virus-infected aedes albopictus (mosquito) cells. | the infection of tissue-cultured aedes albopictus (mosquito) cells by an alphavirus ultimately results in a persistently infected cell population which can be maintained in the laboratory for years. one characteristic of this culture is that it will not support the replication of superinfecting homologous virus. we have previously shown that mosquito cells persistently infected with sindbis virus produce an antiviral agent which when applied to uninfected mosquito cells suppresses sindbis virus ... | 1986 | 3951024 |
| persistent infection of aedes albopictus c6/36 cells by bunyamwera virus. | two cell lines persistently infected with bunyamwera virus have been established from the c6/36 clone of aedes albopictus cells. the cells express bunyamwera virus antigens as detected by immunofluorescence and are resistant to superinfection with bunyamwera virus and other bunyaviruses, but not dugbe virus (nairovirus) nor vesicular stomatitis virus. the virus released from the persistently infected cells developed an altered cloudy or "bull's-eye" plaque morphology with increasing passage leve ... | 1986 | 3952984 |
| susceptibility of aedes albopictus to infection with eastern equine encephalomyelitis virus. | we examined susceptibility of a strain of aedes albopictus from houston, texas to experimental infection with eastern equine encephalomyelitis (eee) virus. after 15 days of extrinsic incubation, all ae. albopictus examined by the cell culture assay and fluorescent antibody staining were infected but only 57% (4/7) of the mosquitoes that refed transmitted virus by bite. data supported the concept of a salivary gland infection barrier to eee virus in ae. albopictus and the conclusion that virus re ... | 1990 | 2164563 |
| failure to detect natural transovarial transmission of dengue viruses by aedes aegypti and aedes albopictus (diptera: culicidae). | | 1985 | 4009622 |
| a new arbovirus from aedes albopictus, an asian mosquito established in the united states. | ten strains of a new arbovirus belonging to the bunyamwera group (bunyaviridae) were recovered from field-collected aedes albopictus mosquitoes in potosi, missouri. this evidence indicates that this species may serve as an arbovirus vector in the united states. the urban-suburban distribution, aggressive biting behavior, and broad viral susceptibility of ae. albopictus may lead to the transmission of viruses of known public health importance and perhaps of viruses hitherto not transmitted to hum ... | 1990 | 2270489 |
| conformational changes at ph 6 on the cell surface of semliki forest virus-infected aedes albopictus cells. | the mechanism of semliki forest virus-induced fusion from within at low ph was studied in aedes albopictus cells. the fusion was found to occur in at least two steps, namely, a fast initial step which is ph dependent and temperature independent, and a second slower process which is ph independent and temperature dependent. the initiation step induced by low ph exposure constitutes an irreversible conformational change of a protein probably of viral origin located at the cell surface. | 1985 | 4060582 |
| sindbis virus mutants able to replicate in methionine-deprived aedes albopictus cells. | previous work from this laboratory has shown that the replication of sindbis virus (sv) in aedes albopictus cells is sensitive to methionine withdrawal. this sensitivity is thought to reflect a diminished concentration of s-adenosylmethionine (ado met) resulting from methionine starvation. serial passage of sv on ae. albopictus cells maintained in low concentrations of methionine gave rise to a population of mutants whose replication in mosquito cells was resistant to methionine starvation. in v ... | 1985 | 4060595 |
| protein synthesis in cells infected by chilo iridescent virus (iridovirus, type 6). | synthesis of the polypeptides induced in civ-infected cells was studied using radiolabelled methionine in a permissive cell line of choristoneura fumiferana. analysis of labelled cell extracts by high resolution polyacrylamide gel electrophoresis (page) revealed the sequential appearance of at least 28 structural and non-structural polypeptides in permissive conditions. this was confirmed by analysis of protein synthesis in non-permissive cell lines of lymantria dispar, aedes albopictus or by th ... | 1985 | 4062562 |
| expression of recombinant vaccinia virus-derived alphavirus proteins in mosquito cells. | a recombinant vaccinia virus strain which contains and expresses a 26s cdna insert encoding sindbis virus structural proteins (vv:3s) was used to infect a continuous line of aedes albopictus mosquito cells. there were not visible cytopathic effects due to the virus infection and the cells continued to grow normally. however, examination of the proteins present in the cytoplasm of the infected cells with sindbis virus-specific antisera revealed that sindbis virus proteins were being synthesized a ... | 1985 | 4067584 |
| two monoclonal antibodies against la crosse virus show host-dependent neutralizing activity. | mammalian and arthropod cell cultures were used to assess the neutralizing activity of six monoclonal antibodies specific for the g1 glycoprotein of la crosse virus. four antibodies, two neutralizing and two non-neutralizing, showed no host-dependent differences, giving similar results when post-treatment infectivity was determined using either aedes albopictus cells or bhk-21 cells. for two other antibodies, however, dissimilar activities were observed between the vertebrate and invertebrate ce ... | 1985 | 4067586 |
| replication of mayaro virus in aedes albopictus cells: an electron microscopic study. | the replication of mayaro virus in aedes albopictus cells, was studied by electron microscopy at various times post-infection. in infected cells we observed the presence of cytoplasmic vesicles containing viral nucleocapsids and mature virus particles but at no time did we detect virus budding into such vacuoles. budding of virus through plasma membrane was rarely observed. our results are discussed considering the possibility of the release of virus particles to the extracellular space by exocy ... | 1989 | 2539797 |
| studies on the pathogenesis of bovine ephemeral fever in sentinel cattle. i. virology and serology. | twenty-two sentinel cattle were observed daily during an outbreak of ephemeral fever on a dairy farm in eastern australia in the summer of 1981-82. of the 22 cattle, 9 developed clinical ephemeral fever. none developed sub-clinical infection. the pattern of the epidemic was a single index case followed 10 days later by the main epidemic wave which lasted for 7 days. this wave stopped when there were still 14 uninfected susceptible animals remaining in the sentinel group, and when biting flies we ... | 1985 | 4095897 |
| morphological variants of sindbis virus obtained from infected mosquito tissue culture cells. | tissue-cultured aedes albopictus cells infected with morphologically homogeneous sindbis virus were found to produce progeny virions which could be divided into three classes based on size. the thickness of the envelope was constant on all three sizes of progeny virions suggesting that the variability in size rested with the viral nucleocapsid. it is suggested that the three classes of virions have icosahedral nucleocapsids composed of common subunits organized in decreasing triangulation number ... | 1973 | 4128381 |
| host-induced modification of dengue-2 virus surface antigens in aedes albopictus cells. | | 1973 | 4132537 |
| susceptibility of aedes albopictus mosquito cell line to some arboviruses. | | 1973 | 4147144 |
| replication of dengue virus type 2 in aedes albopictus cell culture. | the replication of type 2 dengue (d-2) virus in aedes albopictus (aal) mosquito cell cultures differed from that in vertebrate (llc-mk2) rhesus monkey kidney cells. virus readily replicated in aal cells at either 30 or 37 c, but had no apparent effect on the host cell. persistent infection was established with continual virus production for at least 6 months, although the virulence of progeny virus for both suckling mice and llc-mk2 cells became attenuated. density gradient analysis of infected ... | 1973 | 4201188 |
| protein kinase and phosphoproteins of vesicular stomatitis virus. | protein kinases of similar but not identical activity were found associated with vesicular stomatitis (vs) virions grown in mouse l cells, primary chicken embryo (ce) cells, and bhk-21 cells, as well as being present in vs virions grown in hela and aedes albopictus cells. the virion kinase preferentially phosphorylated the nucleocapsid ns protein in vitro and to a lesser extent the envelope m protein. other virion proteins were phosphorylated in vitro only after drastic detergent treatment. part ... | 1974 | 4359419 |
| low humidity water-retention ability in eretmapodites chrysogaster and aedes albopictus. | | 1967 | 4382866 |
| experimental studies with chikungunya virus in aedes aegypti and aedes albopictus. | | 1967 | 4383887 |
| meiosis in the male of culex pipiens and aedes albopictus and fertilization in the culex pipiens-complex. | | 1971 | 4398667 |
| [viral specific rna synthesis in sindbis infected "aedes albopictus" mosquito cells i. - evidence for a 18s rna species in infected cells (author's transl)]. | | 1974 | 4463758 |
| modulation in response to temperature of mayaro virus proteosynthesis in aedes albopictus cells. | incubation of aedes albopictus cells infected with mayaro virus at 37 degrees c causes inhibition of virus replication. during the first hour post infection (p.i.) incubation at 37 degrees c inhibited cellular and virus proteosynthesis. a preferential translation of heat shock proteins 82 kd and 70 kd was observed. after incubations longer than 1 hr at 37 degrees c, a switch to normal pattern of cell protein synthesis occurred without recovery of virus proteosynthesis. in addition, preferential ... | 1989 | 2570510 |
| effect of amino acids on growths of singh's aedes albopictus cells and japanese encephalitis virus. | | 1973 | 4587473 |
| semliki forest virus replication in cultured aedes albopictus cells: studies on the establishment of persistence. | | 1974 | 4610090 |
| clinical observations of mosquito bite reactions in man: a survey of the relationship between age and bite reaction. | to evaluate the mechanism of mosquito bite reaction in man, the reaction to aedes albopictus was observed in 162 subjects ranging in age between 1 to 68 years old. bite reactions were found to consist of both an immediate and a delayed reaction. the eruption and time course of the immediate reaction were consistent with type i hypersensitivity. the eruption and time course of the delayed reaction were consistent with cutaneous basophil hypersensitivity. positive rates of immediate reaction incre ... | 1989 | 2571626 |
| singh's aedes albopictus cell cultures as helper cells for the adaptation of obodhiang and kotonkan viruses of the rabies serogroup to some vertebrat cell cultures. | multiplication of rabies serogroup viruses, obodhiang and kotonkan, was induced in vertebrate cell cultures by using singh's aedes albopictus cell cultures as "helper cells." | 1973 | 4633481 |
| studies of interferon production in aedes albopictus mosquito cells. | | 1973 | 4683227 |
| prevalence of aedes aegypti and aedes albopictus and observations on the ecology of dengue haemorrhagic fever in several areas of thailand. | | 1973 | 4718121 |
| induction of an interferon-like substance in persistently infected aedes albopictus cells. | | 1973 | 4735533 |
| homologous viral interference in aedes albopictus cultures chronically infected with sindbis virus. | complete homologous interference is demonstrated in cultures of aedes albopictus cells chronically infected with sindbis virus. the interference occurred before there was any detectable rna synthesis by the superinfecting virus. | 1973 | 4735592 |
| update: aedes albopictus infestation--united states. | | 1986 | 3093836 |
| hybridization between aedes aegypti and aedes albopictus in malaysia. | | 1973 | 4749074 |
| field and laboratory observations on landing and biting periodicities of aedes albopictus (skuse). | | 1973 | 4749076 |
| aedes mascarensis macgregor on mauritius. i. ecology in relation to aedes albopictus (skuse) (diptera: culicidae). | | 1973 | 4779920 |
| temperature-sensitive virus from aedes albopictus cells chronically infected with sindbis virus. | cultures of aedes albopictus cells persistently infected with wild-type sindbis virus (sv-w) give rise to small plaque-forming mutants which are also temperature sensitive. these mutants, designated sv-c, are neutralized by antiserum produced against sv-w. mutant ts clones were isolated from sv-c by plaque purification. after serial undiluted passage in bhk or mosquito cells, each of the clones gave rise to ts(+) revertants which, however, remained mutant with respect to plaque morphology. ninet ... | 1974 | 4855741 |
| adaptation of singh's aedes albopictus (atc-15) cell line to media with calf and goat sera and their susceptibility to infection with some arboviruses. | | 1971 | 5103347 |
| cross-insemination frequencies between strains of aedes albopictus and members of the aedes scutellaris group. | | 1971 | 5118866 |
| 3. rna of uninfected and sindbis virus-infected aedes albopictus cells. | | 1971 | 5142328 |
| observations on the host range and feeding preferences of aedes albopictus (skuse). | | 1971 | 5153593 |
| experimental infection of aedes aegypti and aedes albopictus with dengue viruses. | | 1971 | 5159140 |
| observations on possible competitive displacement between populations of aedes aegypti linnaeus and aedes albopictus skuse in calcutta. | the possibility of competitive displacement in calcutta between aedes aegypti, a known vector of arboviruses, and a. albopictus, a suspected vector, was explored by general collections of immature stages from all types of breeding-places and by exposing oviposition traps in tenement houses, and gardens in urban, suburban, and rural environments. a. aegypti was predominant in houses and tenements in urban areas, but a. albopictus was not excluded. both species occurred in about equal densities in ... | 1967 | 5301385 |
| isolation of dengue viruses in aedes albopictus cell cultures. | | 1969 | 5307611 |
| aedes aegypti (l.) and aedes albopictus (skuse) in singapore city. 1. distribution and density. | the distribution and density of ae. aegypti and ae. albopictus in singapore were assessed from extensive larval surveys carried out from 1966 to 1968 to evaluate their respective roles in the epidemiology of dengue haemorrhagic fever and to study their ecology in the urban areas. ten urban areas where the majority of dengue haemorrhagic fever cases occurred were surveyed.the results showed that both species were common in the city, with ae. aegypti being the dominant species. the distribution of ... | 1971 | 5316745 |
| aedes aegypti (l.) and aedes albopictus (skuse) in singapore city. 2. larval habitats. | detailed information on the breeding habitats of ae. aegypti and ae. albopictus is necessary when planning programmes for their control. the larval habitats of the two species in 10 city areas were counted and classified according to type, frequency of occurrence, location, and function. of all the breeding habitats recorded 95% were domestic containers. the most common ae. aegypti breeding habitats were ant traps, earthenware jars, bowls, tanks, tin cans, and drums, ant traps being the most com ... | 1971 | 5316746 |