| differentiation of tomato yellow leaf curl virus and tomato yellow leaf curl sardinia virus using real-time taqman pcr. | during the past four decades, tomato yellow leaf curl disease has become one of the major constraints in tomato production worldwide. in the mediterranean basin, several isolates from two major begomovirus species are involved in outbreaks and persistent epidemics. a real-time taqman pcr assay was developed and evaluated for the rapid and multiplex detection and differentiation of two begomoviruses often found in mixed infections in the region, tomato yellow leaf curl virus (tylcv) and tomato ye ... | 2010 | 20153376 |
| prevalence of endosymbionts in bemisia tabaci populations and their in vivo sensitivity to antibiotics. | bemisia tabaci can harbor both primary and secondary endosymbionts, and the specific endosymbionts can differ among different b. tabaci biotypes. this study determined (1) the prevalence of the primary endosymbiont portiera aleyrodidarum and secondary endosymbionts arsenophonus and wolbachia in two invasive biotypes (b and q) and one indigenous biotype (cv) in china and (2) the in vivo effect of three antibiotics (tetracycline, ampicillin trihydrate, and rifampicin) against the endosymbionts; if ... | 2010 | 20217091 |
| integrated approach to tylcd management in sardinia (italy). | in sardinia (italy) tomato yellow leaf curl disease (tylcd) re-emerged in 2003, after a period of decline, producing severe yield losses in protected tomato crops. this epidemic outbreak highlighted the inadequacy of the approach to tylcd management based chiefly on the chemical control of its vector, the whitefly bemisia tabaci, and the use of 40-mesh nets for greenhouse screening. to evaluate the reliability of alternative practices for implementation within ip&dm programmes, in 2007 and 2008 ... | 2009 | 20222567 |
| an epidemiological survey of tylcd in southern sardinia (italy). | tomato yellow leaf curl sardinia virus (tylcsv) and tomato yellow leaf curl virus (tylcv) are among the most virulent pathogens of greenhouse tomatoes in sardinia (italy). to investigate the relationship between seasonal population trends of the vector bemisia tabaci and the dynamics of tylcd spread to susceptible crops, between may and october 2007 we carried out a survey in a tomato growing area located in the south of the island. on three farms specialized in the production of fresh market to ... | 2009 | 20222569 |
| severe outbreak of tomato yellow leaf curl sardinia virus on pepper in southern italy. | during summer and autumn 2008 a severe outbreak of pepper leaf curl disease (plcd) was observed in pepper crops under plastic tunnels in the ionic coast of basilicata region. its incidence reached, in some cases, values close to 50%. the beginning of infections was recorded along the perimeter of the tunnels, where it reached a percentage of almost 100%. the infection then progressively spread towards the central areas of the greenhouses. large populations of whiteflies, identificated as bemisia ... | 2009 | 20222578 |
| seasonal trends of whitefly populations in a mediterranean tomato growing area. | the whiteflies bemisia tabaci and trialeurodes vaporariorum and their associated viruses constitute a major threat to tomato crops in the mediterranean region. continuous host availability and mild climate are thought to be among the factors contributing to the outbreaks of whitefly-related problems in this area. we carried out a year-long survey to investigate the relative contribution of different plants, agricultural and not, and indoor/outdoor crops as hosts of the two whiteflies and the tom ... | 2009 | 20222589 |
| biological agents for whitefly control in sardinian greenhouse tomatoes. | to evaluate the effectiveness of alternative options for biocontrol of whiteflies in greenhouse tomatoes, an experiment was carried out during the cropping season 2005-2006 in one of sardinia's major horticultural districts (s. margherita di pula, cagliari, italy). twelve long-cycle and 17 short-cycle tomato crops (8 autumn and 9 spring crops) were surveyed. all of them were treated for insect pest control at the beginning of the growing season, but in 19 out of 29 cases whitefly natural enemies ... | 2009 | 20222592 |
| co-infection and localization of secondary symbionts in two whitefly species. | whiteflies are cosmopolitan phloem-feeding pests that cause serious damage to many crops worldwide due to direct feeding and vectoring of many plant viruses. the sweetpotato whitefly bemisia tabaci (gennadius) and the greenhouse whitefly trialeurodes vaporariorum (westwood) are two of the most widespread and damaging whitefly species. to complete their unbalanced diet, whiteflies harbor the obligatory bacterium portiera aleyrodidarum. b. tabaci further harbors a diverse array of secondary symbio ... | 2010 | 20462452 |
| host range and complete genome sequence of cucurbit chlorotic yellows virus, a new member of the genus crinivirus. | cucurbit chlorotic yellows virus (ccyv) causes chlorotic yellows on cucumber (cucumis sativus) and melon (cucumis melo) and is transmitted by bemisia tabaci biotype b and q whiteflies. to characterize the host range of ccyv, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. all tested cucumis spp. except cucumis anguria and cucumis zeyheri were systemically infected with ccyv, although infection rates varied among species. citrullus lanatus, cucurbita pepo, and ... | 2010 | 20465411 |
| legume yellow mosaic viruses: genetically isolated begomoviruses. | summary: the yellow mosaic diseases of a number of legumes across southern asia are caused by four species of whitefly-transmitted geminiviruses (genus begomovirus, family geminiviridae): mungbean yellow mosaic virus, mungbean yellow mosaic india virus, dolichos yellow mosaic virus and horsegram yellow mosaic virus. they cause losses to a number of important pulse crops, a major source of dietary protein in the region. the viruses have host ranges limited to plants of the family fabaceae and eff ... | 2007 | 20507504 |
| induced defense by bemisia tabaci biotype b (hemiptera: aleyrodidae) in tobacco against myzus persicae (hemiptera: aphididae). | myzus persicae and bemisia tabaci are serious pests of tobacco and can occur simultaneously on the same plant. we found that tobacco plants infested by whiteflies had fewer aphids than those without whiteflies. to determine whether b. tabaci feeding could induce plant defense against aphids locally and systemically, we determined the effects of b. tabaci on several biological parameters of m. persicae on tobacco. infestation of b. tabaci nymphs reduced survival rates of m. persicae by 30.0%. in ... | 2010 | 20550802 |
| effects of food deprivation on host feeding and parasitism of whitefly parasitoids. | most whitefly parasitoids kill whiteflies in one of two ways, by piercing the whitefly body with the ovipositor and laying an egg or by piercing the body and feeding on host fluids. the effects of food deprivation on host-feeding and parasitizing capacity of the whitefly parasitoids eretmocerus melanoscutus and encarsia formosa (hymenoptera: aphelinidae) were determined in this study. we investigated the effects of various food-deprivation durations (0, 3, 6, 10, and 24 h, and 20% honey solution ... | 2010 | 20550806 |
| change in the biotype composition of bemisia tabaci in shandong province of china from 2005 to 2008. | certain biotypes of the bemisia tabaci (gennadius) (hemiptera: aleyrodidae) complex cause extensive damage and are important pests and virus vectors in agricultural crops throughout the world. among the most invasive and well studied are the b and q biotypes. recent reports in shandong province, china, have indicated that the q biotype was introduced there in approximately 2005, whereas the b biotype has been established there for approximately 10 yr. even so, the present distribution of the two ... | 2010 | 20550819 |
| tomato yellow leaf curl virus, the intracellular dynamics of a plant dna virus. | summary tomato yellow leaf curl virus is a geminivirus, transmitted by whitefly (bemisia tabaci) and causing the most destructive disease of tomato throughout the mediterranean region, the middle east and the tropical regions of africa and central america. affected plants produce either no fruits or a few small fruits. since it is an ssdna virus which replicates in the host cell nucleus, the molecular mechanisms involved in the viral nuclear import have been the focus of our studies in recent ye ... | 2003 | 20569358 |
| tomato yellow leaf curl viruses: ménage à trois between the virus complex, the plant and the whitefly vector. | tomato yellow leaf curl disease (tylcd) is one of the most devastating viral diseases affecting tomato crops in tropical, subtropical and temperate regions of the world. here, we focus on the interactions through recombination between the different begomovirus species causing tylcd, provide an overview of the interactions with the cellular genes involved in viral replication, and highlight recent progress on the relationships between these viruses and their vector, the whitefly bemisia tabaci. t ... | 2010 | 20618703 |
| the transmission efficiency of tomato yellow leaf curl virus by the whitefly bemisia tabaci is correlated with the presence of a specific symbiotic bacterium species. | tomato yellow leaf curl virus (tylcv) (geminiviridae: begomovirus) is exclusively vectored by the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae). tylcv transmission depends upon a 63-kda groel protein produced by the vector's endosymbiotic bacteria. b. tabaci is a species complex comprising several genetically distinct biotypes that show different secondary-symbiont fauna. in israel, the b biotype harbors hamiltonella, and the q biotype harbors wolbachia and arsenophonus. both biot ... | 2010 | 20631135 |
| an invasive whitefly feeding on a virus-infected plant increased its egg production and realized fecundity. | plant-pathogenic begomoviruses have a complex association with their insect vectors. the interactions of begomoviruses and reproduction of their vectors are poorly understood. bemisia tabaci is known to transmit many begomoviruses, and the spread of b. tabaci, especially the b and q 'biotypes', has been accompanied by the epidemics of begomoviruses. one of these identified disease-causing agents was tomato yellow leaf curl china virus (tylccnv). | 2010 | 20676356 |
| insecticide resistance in field populations of bemisia tabaci (hemiptera: aleyrodidae) in west africa. | the tobacco whitefly, bemisia tabaci gennadius (hemiptera: aleyrodidae), has developed a high degree of resistance to several chemical classes of insecticides throughout the world. to evaluate the resistance status in west africa, eight insecticides from different chemical families were tested using the leaf-dip method on four field populations collected from cotton in benin, togo and burkina faso. | 2010 | 20721972 |
| neonicotinoid insecticides: historical evolution and resistance mechanisms. | the use ofneonicotinoid insecticides has grown considerably since their introduction in 1990s. they are used extensively for the control of agriculturally important crop pests and also in the control of cat and dog fleas. imidacloprid exploited through an elaborated structural and substituent optimization of nithiazine was launched to market in 1990. the selectivity ofneonicotinoid compounds for insect species has been attributed to their binding on nicotinic acetylcholine receptors in which the ... | 2010 | 20737790 |
| incidence and characterisation of resistance to neonicotinoid insecticides and pymetrozine in the greenhouse whitefly, trialeurodes vaporariorum westwood (hemiptera: aleyrodidae). | trialeurodes vaporariorum (westwood), also known as the greenhouse whitefly, is a serious pest of protected vegetable and ornamental crops in most temperate regions of the world. neonicotinoid insecticides are used widely to control this species, although resistance has been reported and may be becoming widespread. | 2010 | 20799247 |
| distribution and dynamics of bemisia tabaci invasive biotypes in central china. | the tobacco whitefly, bemisia tabaci (gennadius), causes severe crop losses in many agricultural systems. the worst of these losses are often associated with the invasion and establishment of specific whitefly biotypes. in a comprehensive survey of biotypes present in central china between 2005 and 2007, we obtained 191 samples of b. tabaci from 19 districts in hubei province and its surrounds. biotypes were identified by rapd-pcr and by sequencing the mitochondrial cytochrome oxidase i gene (mt ... | 2011 | 20822556 |
| flufenerim, a novel insecticide acting on diverse insect pests: biological mode of action and biochemical aspects. | a new chemical compound was tested for its insecticidal activity against several major insect pests. the compound, called "flufenerim", has a core pyrimidine structure and an unknown mode of action and showed potent activity against the sweet potato whitefly bemisia tabaci (gennadius), the green peach aphid myzus persicae (sulzer), and the african cotton leafworm spodoptera littoralis (boisduval); however, it did not show any activity against two thrips species: western flower thrips frankliniel ... | 2010 | 20958045 |
| phylogenetic analysis and inflow route of tomato yellow leaf curl virus (tylcv) and bemisia tabaci in korea. | tomato yellow leaf curl virus (tylcv) is a member of the genus begomovirus of the family geminiviridae, members of which are characterized by closed circular single-stranded dna genomes of 2.7-2.8 kb in length, and include viruses transmitted by the bemisia tabaci whitefly. no reports of tylcv in korea are available prior to 2008, after which tylcv spread rapidly to most regions of the southern korean peninsula (gyeongsang-do, jeolla-do and jeju-do). fifty full sequences of tylcv were analyzed i ... | 2010 | 20981497 |
| prevalence of wolbachia supergroups a and b in bemisia tabaci (hemiptera: aleyrodidae) and some of its natural enemies. | wolbachia, a bacterial symbiont, is maternally transmitted in arthropods and nematodes. we report a systematic survey of wolbachia taxonomy in the sweetpotato whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae), and in some of its natural enemies. for the first time, wolbachia infections in b. tabaci are correlated with various whitefly genetic groups, host plants, and natural enemies as well as with geographical regions. polymerase chain reaction using 16s rdna and fisz genes revealed ... | 2010 | 21061989 |
| release of hormones from conjugates: chloroplast expression of β-glucosidase results in elevated phytohormone levels associated with significant increase in biomass and protection from aphids or whiteflies conferred by sucrose esters. | transplastomic tobacco (nicotiana tabacum) plants expressing β-glucosidase (bgl-1) show modified development. they flower 1 month earlier with an increase in biomass (1.9-fold), height (1.5-fold), and leaf area (1.6-fold) than untransformed plants. trichome density on the upper and lower leaf surfaces of bgl-1 plants increase by 10- and 7-fold, respectively, harboring 5-fold more glandular trichomes (as determined by rhodamine b staining), suggesting that bgl-1 lines produce more sugar esters th ... | 2010 | 21068365 |
| acquisition of lettuce infectious yellows virus by bemisia tabaci perturbs the transmission of lettuce chlorosis virus. | viruses in the genus crinivirus infect diverse plant species and are transmitted by specific whitefly vectors, but the basis for vector specific transmission remains poorly understood. here, we demonstrated that purified virion preparations of lettuce chlorosis virus (lcv) contained filamentous particles that were consistently transmitted to plants by whiteflies (bemisia tabaci biotypes a and b) following membrane feeding, suggesting that the preparations contained biologically active virions wi ... | 2011 | 21211541 |
| insecticidal activity against bemisia tabaci biotype b of peel essential oil of citrus sinensis var. pear and citrus aurantium cultivated in northeast brazil. | the fumigant action of peel essential oils of citrus sinensis var. pear (pear orange = po) and c. aurantium (bitter orange = bo) from the northeast of brazil were evaluated against bemisia tabaci biotype b and compared with eugenol as a positive control. the oil concentration in the po at 8.5 microl/l of air caused 97% mortality, while the oil concentration of bo at 9.5 microl/l of air caused 99% mortality. however, the lc50 estimates for both oils (lc50 = 3.80 microl/l of air for po and lc50 = ... | 2010 | 21213990 |
| global analysis of the transcriptional response of whitefly to tomato yellow leaf curl china virus reveals the relationship of coevolved adaptations. | the begomoviruses are the largest and most economically important group of plant viruses transmitted exclusively by the whitefly bemisia tabaci in a circulative, persistent manner. the circulation of the viruses within the insect vectors involves complex interactions between virus and vector components; however, the molecular mechanisms of these interactions remain largely unknown. here we investigated the transcriptional response of the invasive b. tabaci middle east-asia minor 1 species to tom ... | 2011 | 21270146 |
| genetic identity of the bemisia tabaci species complex and association with high cotton leaf curl disease (clcud) incidence in pakistan. | the cotton whitefly, bemisia tabaci (gennadius), is a cryptic species complex, and members of the complex have become serious pests in pakistan because of their feeding and their ability to transmit cotton leaf curl virus (clcuv). here, an analysis was made of the identity of b. tabaci collected from cotton and a range of non-cotton hosts in the cotton-growing zones in punjab and sindh, the main cotton-producing provinces of pakistan, using a portion of the mitochondrial cytochrome oxidase 1 gen ... | 2010 | 21308956 |
| extraordinary resistance to insecticides reveals exotic q biotype of bemisia tabaci in the new world. | a strain of the whitefly bemisia tabaci (gennadius) possessing unusually high levels of resistance to a wide range of insecticides was discovered in 2004 in the course of routine resistance monitoring in arizona. the multiply resistant insects, collected from poinsettia (euphorbia pulcherrima willd. ex klotzsch) plants purchased at a retail store in tucson, were subjected to biotype analysis in three laboratories. polyacrylamide gel electrophoresis of naphthyl esterases and sequencing of the mit ... | 2010 | 21309242 |
| further insights into the strange role of bacterial endosymbionts in whitefly, bemisia tabaci: comparison of secondary symbionts from biotypes b and q in china. | the percentage infection of secondary symbionts (ss) (wolbachia, arsenophonus, rickettsia, hamiltonella, fritschea and cardinium) in the exotic bemisia tabaci (genn.) invaders, commonly known as biotypes b and q from china, were determined by pcr. in total, 373 biotype b and 1830 biotype q individuals were screened for the presence of ss. biotype b was more abundant than biotype q from 2005 to 2006, and biotype q was more abundant from 2007 to 2009. each of the ss, with the exception of fritsche ... | 2011 | 21329550 |
| resistance to tomato yellow leaf curl virus accumulation in the tomato wild relative solanum habrochaites associated with the c4 viral protein. | tomato yellow leaf curl disease (tylcd) is a severe threat to tomato crops worldwide and is caused by tomato yellow leaf curl virus (tylcv) and several other begomoviruses (genus begomovirus, family geminiviridae). host plant resistance is the best tylcd control method but limited sources of resistance are available. in this study, two solanum habrochaites tylcd-resistance sources, eelm-388 and eelm-889, were found after a wide germplasm screening and were further characterized. a consistent res ... | 2011 | 21405986 |
| rapid spread of a bacterial symbiont in an invasive whitefly is driven by fitness benefits and female bias. | maternally inherited bacterial symbionts of arthropods are common, yet symbiont invasions of host populations have rarely been observed. here, we show that rickettsia sp. nr. bellii swept into a population of an invasive agricultural pest, the sweet potato whitefly, bemisia tabaci, in just 6 years. compared with uninfected whiteflies, rickettsia-infected whiteflies produced more offspring, had higher survival to adulthood, developed faster, and produced a higher proportion of daughters. the symb ... | 2011 | 21474763 |
| symbiont diversity and non-random hybridization among indigenous (ms) and invasive (b) biotypes of bemisia tabaci. | the whitefly bemisia tabaci (hemiptera: aleyrodidae) is a worldwide pest and a vector of numerous plant viruses. b. tabaci is composed of dozens of morphologically indistinguishable biotypes and its taxonomic status is still controversial. this phloem-feeder harbours the primary symbiont portiera aleyrodidarum and potentially six secondary symbionts: cardinium, arsenophonus, hamiltonella, rickettsia, wolbachia and fritschea. in the southwest indian ocean, la réunion hosts two biotypes of this sp ... | 2011 | 21476990 |
| complete nucleotide sequence of croton yellow vein mosaic virus and dna-β associated with yellow vein mosaic disease of jatropha gossypifolia in india. | a severe yellow vein mosaic disease was noticed on several jatropha gossypifolia plants growing nearby agriculture fields at lucknow, india. diseased plants exhibited yellow vein mosaic, leaf deformation, vein swelling and stunting. a population of whiteflies (bemisia tabaci) was also noticed in the vicinities; therefore, begomovirus infection was suspected. to confirm begomovirus association, total dna was isolated from symptomatic leaf samples and subjected to pcr using dna-a, dna-b and dna-β- ... | 2011 | 21479677 |
| contact and fumigant toxicity of plant essential oils and efficacy of spray formulations containing the oils against b- and q-biotypes of bemisia tabaci. | background: the contact + fumigant toxicity of 92 plant essential oils and control efficacy of 18 experimental spray formulations containing nine selected essential oils (0.5 and 0.1% sprays) and six commercial insecticides to females from b- and q-biotypes of bemisia tabaci were evaluated using vapour-phase mortality and spray bioassays. results: garlic and oregano (lc(50) , 0.15 ml cm(-3) ) were the most toxic oils against b- and q-biotype females. strong fumigant toxicity to both biotype fema ... | 2011 | 21480461 |
| genetic diversity, geographical range and origin of bemisia tabaci (hemiptera: aleyrodidae) indian ocean ms. | the whitefly bemisia tabaci is a pest vector of begomoviruses on crops worldwide. bemisia tabaci is composed of a complex of cryptic species which barely interbreed. an exception is the ms from the south west indian ocean (swio), which crosses in low proportions with the exotic b. the ms, together with b and q is part of the same phylogenetic clad. to infer the genetic structure, the geographical range and putative origin of this putative species, microsatellite data and mitochondrial dna (cytoc ... | 2011 | 21492491 |
| effects of selected fertilizers on the life history of bemisia tabaci (hemiptera: aleyrodidae) biotype b. | we tested the effects among a purportedly sustainable water-soluble fertilizer, a conventional water-soluble fertilizer, an alternation of these, a controlled-release fertilizer, and a clear water control on the life-history traits of sweetpotato whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae; =bemisia argentifolii bellows & perring) biotype b reared on poinsettia (euphorbia pulcherrima willdenow ex klotzch). free amino acids in petioles were measured to estimate plant nutrient ass ... | 2011 | 21510203 |
| molecular characterization and oxidative stress response of an intracellular cu/zn superoxide dismutase (cuznsod) of the whitefly, bemisia tabaci. | superoxide dismutases (sods) are important for the survival of insects under environmental and biological stresses; however, little attention has been devoted to the functional characterization of sods in whitefly. in this study, an intracellular copper/zinc superoxide dismutase of whitefly (bemisia tabaci) (bt-cuznsod) was cloned. sequence analysis indicated that the full length cdna of bt-cuznsod is of 907 bp with a 471 bp open reading frame encoding 157 amino acids. the deduced amino acid seq ... | 2011 | 21541989 |
| biological activity of natural phytoecdysteroids from ajuga iva against the sweetpotato whitefly bemisia tabaci and the persea mite oligonychus perseae. | background: ecdysteroids are steroid hormones that control moulting and govern several changes during metamorphoses in arthropods. the discovery of the same molecules (phytoecdysteroids) in several plant species displayed a wide array of rather beneficial agricultural impact. many representatives of the genus ajuga plants contain phytoecdysteroids with a 5β-7-ene-6-one system exhibiting physiological activities in insects. results: by means of chromatographic (silica gel column, tlc) and lc-ms, ... | 2011 | 21604353 |
| whitefly resistance traits derived from the wild tomato solanum pimpinellifollium affect the preference and feeding behavior of bemisia tabaci and reduce the spread of tomato yellow leaf curl virus. | breeding of tomato genotypes that limit whitefly (bemisia tabaci) access and feeding might reduce the spread of tomato yellow leaf curl virus (tylcv), a begomovirus (genus begomovirus, family geminiviridae) that is the causal agent of tomato yellow leaf curl disease. tylcv is restricted to the phloem and is transmitted in a persistent manner by b. tabaci. the tomato breeding line abl 14-8 was developed by introgressing type iv leaf glandular trichomes and secretion of acylsucroses from the wild ... | 2011 | 21615206 |
| [adult oviposition and larvae feeding behavior of spodoptera litura (lepidoptera: noctuidae) on tobacco plants after infested by b-biotype bemisia tabaci (homoptera: aleyrodidae)]. | to understand the effects of the defense responses of tobacco plants induced by the infesting of b-biotype bemisia tabaci to spodoptera litura, and to explore the mechanisms of the interspecific interactions between b-biotype b. tabaci and s. litura, a laboratory experiment was conducted to study the effects of tobacco plants after infested by b-biotype b. tabaci on the adult oviposition selection and the larvae feeding, anti-feeding, and other feeding behaviors of s. litura. comparing with that ... | 2011 | 21812310 |
| suppressors of rna silencing encoded by the components of the cotton leaf curl begomovirus-betasatellite complex. | begomoviruses (family geminiviridae) are single-stranded dna viruses transmitted by the whitefly bemisia tabaci. many economically important diseases in crops are caused by begomoviruses, particularly in tropical and subtropical environments. these include the betasatellite-associated begomoviruses causing cotton leaf curl disease (clcud) that causes significant losses to a mainstay of the economy of pakistan, cotton. rna interference (rnai) or gene silencing is a natural defense response of pla ... | 2011 | 21751853 |
| a novel synthetic quantification standard including virus and internal report targets: application for the detection and quantification of emerging begomoviruses on tomato. | abstract: background: begomovirus is a genus of phytopathogenic single-stranded dna viruses, transmitted by the whitefly bemisia tabaci. this genus includes emerging and economically significant viruses such as those associated with tomato yellow leaf curl disease, for which diagnostic tools are needed to prevent dispersion and new introductions. five real-time pcrs with an internal tomato reporter gene were developed for accurate detection and quantification of monopartite begomoviruses, includ ... | 2011 | 21819593 |
| adulticidal activity of phthalides identified in cnidium officinale rhizome to b- and q-biotypes of bemisia tabaci. | the residual contact toxicity of three benzofuranoids (z)-butylidenephthalide (1), (3s)-butylphthalide (2), and (z)-ligustilide (3) identified in the rhizome of cnidium officinale (apiaceae) to b- and q-biotype females of bemisia tabaci was evaluated using a leaf-dip bioassay. results were compared with those of eight conventional insecticides. based on 24 h lc(50) values, (z)-butylidenephthalide (254 ppm) and (z)-ligustilide (268 ppm) were more toxic than (3s)-butylphthalide (339 ppm) against b ... | 2011 | 21728333 |
| rickettsia 'in' and 'out': two different localization patterns of a bacterial symbiont in the same insect species. | intracellular symbionts of arthropods have diverse influences on their hosts, and their functions generally appear to be associated with their localization within the host. the effect of localization pattern on the role of a particular symbiont cannot normally be tested since the localization pattern within hosts is generally invariant. however, in israel, the secondary symbiont rickettsia is unusual in that it presents two distinct localization patterns throughout development and adulthood in i ... | 2011 | 21712994 |
| Interspecific Interactions Between Bemisia tabaci Biotype B and Trialeurodes vaporariorum (Hemiptera: Aleyrodidae). | Bemisia tabaci (Gennadius) biotype B and Trialeurodes vaporariorum (Westwood) are invasive whitefly species that often co-occur on greenhouse-grown vegetables in northern China. Although B. tabaci biotype B has been present in China for a relatively short period of time, it has become dominant over T. vaporariorum. We studied the interspecific competitive interactions between the two species in single or mixed cultures at 24 ± 1°C, 40 ± 5% RH, and L14:D10 h photoperiod. Female longevity on toma ... | 2011 | 22182623 |
| Horizontal transmission of the insect symbiont Rickettsia is plant-mediated. | Bacteria in the genus Rickettsia, best known as vertebrate pathogens vectored by blood-feeding arthropods, can also be found in phytophagous insects. The presence of closely related bacterial symbionts in evolutionarily distant arthropod hosts presupposes a means of horizontal transmission, but no mechanism for this transmission has been described. Using a combination of experiments with live insects, molecular analyses and microscopy, we found that Rickettsia were transferred from an insect hos ... | 2011 | 22113034 |
| yellowing disease in zucchini squash produced by mixed infections of cucurbit yellow stunting disorder virus and cucumber vein yellowing virus. | zucchini squash is host to cucurbit yellow stunting disorder virus (cysdv), a member of the genus crinivirus, and cucumber vein yellowing virus (cvyv), a member of the genus ipomovirus, both transmitted by the whitefly bemisia tabaci. field observations suggest the appearance of new symptoms observed on leaves of zucchini squash crops when both viruses were present. when infected during controlled experiments with cysdv only, zucchini plants showed no obvious symptoms and the virus titer decreas ... | 2011 | 21999160 |
| molecular identification of a new begomovirus associated with yellow mosaic disease of jatropha gossypifolia in india. | yellow mosaic disease was observed on jatropha gossypifolia plants growing in kathaupahadi, madhya pradesh, india, and whiteflies (bemisia tabaci) were found in the vicinity. association of a new begomovirus with yellow mosaic disease of j. gossypifolia has been detected by pcr using begomovirus dna-a-specific primers. the complete dna-a genome (~2.7 kb) of this virus isolate was amplified by rolling-circle amplification (rca) followed by digestion with bam hi. the ~2.7-kb amplicons was cloned a ... | 2011 | 21971870 |
| pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, lepidopteran insects, bacterial and viral pathogens. | broad spectrum protection against different insects and pathogens requires multigene engineering. however, such broad spectrum protection against biotic stress is provided by a single protein in some medicinal plants. therefore, tobacco chloroplasts were transformed with the agglutinin gene from pinellia ternata (pta), a widely cultivated chinese medicinal herb. pinellia ternata agglutinin (pta) was expressed up to 9.2% of total soluble protein in mature leaves. purified pta showed similar hemag ... | 2011 | 22077160 |
| oral toxicity of photorhabdus culture media on gene expression of the adult sweetpotato whitefly, bemisia tabaci. | the oral toxicity of culture media of the symbiotic bacteria, photorhabdus temperata, mutually associated with entomopathogenic nematode heterorhabditis megidis and photorhabdus luminescens ssp. laumondii (tt01) mutually associated with heterorhabditis bacteriophora, were investigated in the adults of bemisia tabaci. the oral ingestion of sucrose diet solutions (20%) containing bacteria-free supernatant of the culture media from symbiotic bacteria gradually increased mortalities and was complete ... | 2012 | 22041203 |
| Sweepoviruses cause disease in sweet potato and related Ipomoea spp.: fulfilling Koch's postulates for a divergent group in the genus begomovirus. | Sweet potato (Ipomoea batatas) and related Ipomoea species are frequently infected by monopartite begomoviruses (genus Begomovirus, family Geminiviridae), known as sweepoviruses. Unlike other geminiviruses, the genomes of sweepoviruses have been recalcitrant to rendering infectious clones to date. Thus, Koch's postulates have not been fullfilled for any of the viruses in this group. Three novel species of sweepoviruses have recently been described in Spain: Sweet potato leaf curl Lanzarote virus ... | 2011 | 22073314 |
| molecular characterization of bemisia tabaci populations in tunisia: genetic structure and evidence for multiple acquisition of secondary symbionts. | a survey was conducted during 2009-2010 seasons to identify the distribution of bemisia tabaci (gennadius) biotypes in tunisia. the genetic affiliation of collected populations was determined by polymerase chain reaction (pcr)-restriction fragment-length polymorphism (taqi) of the mitochondrial cytochrom oxidase i (mtcoi) gene. results, validated by sequencing and phylogenetic analysis, allowed the clustering of sampled sweetpotato whiteflies into b and q biotypes. as b. tabaci harbors the obli ... | 2011 | 22251681 |
| insights into the transcriptomics of polyphagy: bemisia tabaci adaptability to phenylpropanoids involves coordinated expression of defense and metabolic genes. | the whitefly bemisia tabaci is a major generalist agricultural pest of field and horticultural crops world-wide. despite its importance, the molecular bases of defense mechanisms in b. tabaci against major plant secondary defense compounds, such as the phenylpropanoids, remain unknown. our experimental system utilized transgenic nicotiana tabacum plants constitutively expressing the pap1/atmyb75 transcription factor which activates relatively specifically the phenylpropanoid/flavonoids biosynthe ... | 2011 | 22212826 |
| biological control strategies for the south american tomato moth (lepidoptera: gelechiidae) in greenhouse tomatoes. | the south american tomato pinworm, tuta absoluta (meyrick) has been introduced into new geographic areas, including the mediterranean region, where it has become a serious threat to tomato production. three greenhouse trials conducted in tomato crops during 2009 and 2010 explored control strategies using the egg-parasitoid trichogramma achaeae nagaraja and nagarkatti compared with chemical control. the effectiveness of the predator nesidiocoris tenuis (reuter) was also tested. in greenhouses wit ... | 2012 | 23356074 |
| high efficient of females of b-type bemisia tabaci as males in transmitting the whitefly-borne tomato yellow leaf curl virus to tomato plant with q-pcr method confirmation. | it has been previously reported that tylcv can be transmitted from viruliferous males to non-viruliferous females and from viruliferous females to non-viruliferous males, but not between insects of the same sex; female whiteflies transmit tylcv-is with higher efficiency than males through symptoms recognition and viral dna identification in tomato test plants (one insect per plant, with 48 h aap and 48 h iap). however, it remains unclear whether non-infected female and male could obtain same vir ... | 2012 | 23336021 |
| [effects of calcium fertilizer on the development, survival, and feeding of b-biotype bemisia tabaci on euphorbia pulcherrima]. | this paper studied the development, survival, and feeding of b-biotype bemisia tabaci on euphorbia pulcherrima under the conditions of 26 +/- 1 degrees c and 60% - 80% relative humidity after applying calcium fertilizer, taking applying fresh water as the control. there existed significant differences in the developmental duration of b. tabaci between treatment applying calcium fertilizer and the control. after applying calcium fertilizer, the egg stage of b. tabaci shortened significantly, and ... | 2012 | 23286011 |
| suppression of terpenoid synthesis in plants by a virus promotes its mutualism with vectors. | vectors often perform better on plants infected with pathogens, and this promotes the spread of pathogens. however, few studies have examined how plant defensive compounds mediate such mutualistic relationships. although tobacco plants are relatively poor host plants for the whitefly bemisia tabaci, tobacco's suitability to the whitefly was substantially increased when infected by the begomovirus tomato yellow leaf curl china virus. the change in suitability was associated with induced terpenoid ... | 2012 | 23279824 |
| time-dose-mortality data and modeling for the entomopathogenic fungus aschersonia placenta against the whitefly bemisia tabaci. | the fungus aschersonia placenta fjsm was evaluated for control of the sweet potato whitefly, bemisia tabaci. bemisia tabaci nymphs (1st-4th instars) on tomato plants in the greenhouse (25-27 °c, 70%-85% relative humidity) were sprayed with suspensions containing 0, 10(4), 10(5), 10(6), 10(7), or 10(8) a. placenta fjsm conidia/ml. mortality of fungus-treated 1st to 3rd instar nymphs ranged from 93% to 100% but was <25% for 4th instar nymphs; the fungus sporulated from 70% to 80% of the fungus-tre ... | 2012 | 23461516 |
| the genome of cardinium cbtq1 provides insights into genome reduction, symbiont motility, and its settlement in bemisia tabaci. | many insects harbor inherited bacterial endosymbionts. although some of them are not strictly essential and are considered facultative, they can be a key to host survival under specific environmental conditions, such as parasitoid attacks, climate changes, or insecticide pressures. the whitefly bemisia tabaci is at the top of the list of organisms inflicting agricultural damage and outbreaks, and changes in its distribution may be associated to global warming. in this work, we have sequenced and ... | 2014 | 24723729 |
| inoculation of tomato plants with rhizobacteria enhances the performance of the phloem-feeding insect bemisia tabaci. | in their natural environment, plants experience multiple biotic interactions and respond to this complexity in an integrated manner. therefore, plant responses to herbivory are flexible and depend on the context and complexity in which they occur. for example, plant growth promoting rhizobacteria (pgpr) can enhance plant growth and induce resistance against microbial pathogens and herbivorous insects by a phenomenon termed induced systemic resistance (isr). in the present study, we investigated ... | 2013 | 23964283 |
| metabolic coevolution in the bacterial symbiosis of whiteflies and related plant sap-feeding insects. | genomic decay is a common feature of intracellular bacteria that have entered into symbiosis with plant sap-feeding insects. this study of the whitefly bemisia tabaci and two bacteria (portiera aleyrodidarum and hamiltonella defensa) cohoused in each host cell investigated whether the decay of portiera metabolism genes is complemented by host and hamiltonella genes, and compared the metabolic traits of the whitefly symbiosis with other sap-feeding insects (aphids, psyllids, and mealybugs). paral ... | 2015 | 26377567 |
| factors affecting population dynamics of maternally transmitted endosymbionts in bemisia tabaci. | while every individual of bemisia tabaci (hemiptera: aleyrodidae) harbors the primary symbiont (p-symbiont) portiera, the infection frequencies of the six secondary symbionts (s-symbionts) including hamiltonella, arsenophonus, cardinium, wolbachia, rickettsia and fritschea vary greatly among different populations. to characterize the factors influencing the infection dynamics of the six s-symbionts in b. tabaci, gene-specific pcr were conducted to screen for the presence of the p-symbiont portie ... | 2012 | 22383972 |
| the evolution of genomic instability in the obligate endosymbionts of whiteflies. | many insects depend on ancient associations with intracellular bacteria to perform essential metabolic functions. these endosymbionts exhibit striking examples of convergence in genome architecture, including a high degree of structural stability that is not typical of their free-living counterparts. however, the recently sequenced genome of the obligate whitefly endosymbiont portiera revealed features that distinguish it from other ancient insect associates, such as a low gene density and the p ... | 2013 | 23542079 |
| comparison of the genome sequences of "candidatus portiera aleyrodidarum" primary endosymbionts of the whitefly bemisia tabaci b and q biotypes. | "candidatus portiera aleyrodidarum" is the primary endosymbiont of whiteflies. we report two complete genome sequences of this bacterium from the worldwide invasive b and q biotypes of the whitefly bemisia tabaci. differences in the two genome sequences may add insights into the complex differences in the biology of both biotypes. | 2013 | 23315735 |
| location of symbionts in the whitefly bemisia tabaci affects their densities during host development and environmental stress. | bacterial symbionts often enhance the physiological capabilities of their arthropod hosts and enable their hosts to expand into formerly unavailable niches, thus leading to biological diversification. many arthropods, including the worldwide invasive whitefly bemisia tabaci, have individuals simultaneously infected with symbionts of multiple genera that occur in different locations in the host. this study examined the population dynamics of symbionts that are located in different areas within b. ... | 2014 | 24632746 |
| female-biased symbionts and tomato yellow leaf curl virus infections in bemisia tabaci. | the female-biased infection of facultative symbionts has been found in bemisia tabaci; however, whether there are any differences in tomato yellow leaf curl virus (tylcv) and obligate symbiont infection rates between females and males is unknown. determining whether such differences exist would be very important for understanding the spread of the plant virus and of the symbionts. we compared both symbiont infection types, including obligate and facultative symbionts, and the rates of tylcv infe ... | 2014 | 24465416 |
| differential temporal changes of primary and secondary bacterial symbionts and whitefly host fitness following antibiotic treatments. | where multiple symbionts coexist in the same host, the selective elimination of a specific symbiont may enable the roles of a given symbiont to be investigated. we treated the mediterranean species of the whitefly bemisia tabaci complex by oral delivery of the antibiotic rifampicin, and then examined the temporal changes of its primary symbiont "candidatus portiera aleyrodidarum" and secondary symbiont "ca. hamiltonella defensa" as well as host fitness for three generations. in adults treated wi ... | 2015 | 26510682 |
| whitefly genome expression reveals host-symbiont interaction in amino acid biosynthesis. | whitefly (bemisia tabaci) complex is a serious insect pest of several crop plants worldwide. it comprises several morphologically indistinguishable species, however very little is known about their genetic divergence and biosynthetic pathways. in the present study, we performed transcriptome sequencing of asia 1 species of b. tabaci complex and analyzed the interaction of host-symbiont genes in amino acid biosynthetic pathways. | 2015 | 26000976 |
| genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly bemisia tabaci. | the whitefly bemisia tabaci is an important agricultural pest with global distribution. this phloem-sap feeder harbors a primary symbiont, "candidatus portiera aleyrodidarum", which compensates for the deficient nutritional composition of its food sources, and a variety of secondary symbionts. interestingly, all of these secondary symbionts are found in co-localization with the primary symbiont within the same bacteriocytes, which should favor the evolution of strong interactions between symbion ... | 2015 | 25887812 |
| cellular and molecular remodelling of a host cell for vertical transmission of bacterial symbionts. | various insects require intracellular bacteria that are restricted to specialized cells (bacteriocytes) and are transmitted vertically via the female ovary, but the transmission mechanisms are obscure. we hypothesized that, in the whitefly bemisia tabaci, where intact bacteriocytes (and not isolated bacteria) are transferred to oocytes, the transmission mechanism would be evident as cellular and molecular differences between the nymph (pre-adult) and adult bacteriocytes. we demonstrate dramatic ... | 2016 | 27358364 |
| pyrosequencing the bemisia tabaci transcriptome reveals a highly diverse bacterial community and a robust system for insecticide resistance. | bemisia tabaci (gennadius) is a phloem-feeding insect poised to become one of the major insect pests in open field and greenhouse production systems throughout the world. the high level of resistance to insecticides is a main factor that hinders continued use of insecticides for suppression of b. tabaci. despite its prevalence, little is known about b. tabaci at the genome level. to fill this gap, an invasive b. tabaci b biotype was subjected to pyrosequencing-based transcriptome analysis to ide ... | 2012 | 22558125 |
| arabidopsis transcriptome changes in response to phloem-feeding silverleaf whitefly nymphs. similarities and distinctions in responses to aphids. | phloem-feeding pests cause extensive crop damage throughout the world, yet little is understood about how plants perceive and defend themselves from these threats. the silverleaf whitefly (slwf; bemisia tabaci type b) is a good model for studying phloem-feeding insect-plant interactions, as slwf nymphs cause little wounding and have a long, continuous interaction with the plant. using the affymetrix ath1 genechip to monitor the arabidopsis (arabidopsis thaliana) transcriptome, 700 transcripts we ... | 2007 | 17189325 |
| silverleaf whitefly induces salicylic acid defenses and suppresses effectual jasmonic acid defenses. | the basal defenses important in curtailing the development of the phloem-feeding silverleaf whitefly (bemisia tabaci type b; slwf) on arabidopsis (arabidopsis thaliana) were investigated. sentinel defense gene rnas were monitored in slwf-infested and control plants. salicylic acid (sa)-responsive gene transcripts accumulated locally (pr1, bgl2, pr5, sid2, eds5, pad4) and systemically (pr1, bgl2, pr5) during slwf nymph feeding. in contrast, jasmonic acid (ja)- and ethylene-dependent rnas (pdf1.2, ... | 2007 | 17189328 |
| the transcript and metabolite networks affected by the two clades of arabidopsis glucosinolate biosynthesis regulators. | in this study, transcriptomics and metabolomics data were integrated in order to examine the regulation of glucosinolate (gs) biosynthesis in arabidopsis (arabidopsis thaliana) and its interface with pathways of primary metabolism. our genetic material for analyses were transgenic plants overexpressing members of two clades of genes (altered tryptophan regulation1 [atr1]-like and myb28-like) that regulate the aliphatic and indole gs biosynthetic pathways (ags and igs, respectively). we show that ... | 2008 | 18829985 |
| plant virus differentially alters the plant's defense response to its closely related vectors. | the whitefly, bemisia tabaci (hemiptera: aleyrodidae), is one of the most widely distributed agricultural pests. in recent years, b. tabaci q has invaded china, and q has displaced b in many areas now. in a number of regions of the world, invasion by b and/or q has been followed by outbreaks of tomato yellow leaf curl virus (tylcv). our previous study showed tylcv directly and indirectly modified the feeding behavior of b. tabaci in favor of q rather than b. | 2013 | 24391779 |
| enhanced methanol production in plants provides broad spectrum insect resistance. | plants naturally emit methanol as volatile organic compound. methanol is toxic to insect pests; but the quantity produced by most of the plants is not enough to protect them against invading insect pests. in the present study, we demonstrated that the over-expression of pectin methylesterase, derived from arabidopsis thaliana and aspergillus niger, in transgenic tobacco plants enhances methanol production and resistance to polyphagous insect pests. methanol content in the leaves of transgenic pl ... | 2013 | 24223989 |
| the facultative symbiont rickettsia protects an invasive whitefly against entomopathogenic pseudomonas syringae strains. | facultative endosymbionts can benefit insect hosts in a variety of ways, including context-dependent roles, such as providing defense against pathogens. the role of some symbionts in defense may be overlooked, however, when pathogen infection is transient, sporadic, or asymptomatic. the facultative endosymbiont rickettsia increases the fitness of the sweet potato whitefly (bemisia tabaci) in some populations through mechanisms that are not yet understood. in this study, we investigated the role ... | 2014 | 25217020 |
| members of wrky group iii transcription factors are important in tylcv defense signaling pathway in tomato (solanum lycopersicum). | transmitted by the whitefly bemisia tabaci, tomato yellow leaf curly virus (tylcv) has posed serious threats to plant growth and development. plant innate immune systems against various threats involve wrky group iii transcription factors (tfs). this group participates as a major component of biological processes in plants. | 2016 | 27717312 |
| a transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters. | the first generation transgenic crops used strong constitutive promoters for transgene expression. however, tissue-specific expression is desirable for more precise targeting of transgenes. moreover, piercing/sucking insects, which are generally resistant to insecticidal bacillus thuringiensis (bt) proteins, have emerged as a major pests since the introduction of transgenic crops expressing these toxins. phloem-specific promoters isolated from banana bunchy top virus (bbtv) were used for the exp ... | 2016 | 27708374 |
| quantification and localization of watermelon chlorotic stunt virus and tomato yellow leaf curl virus (geminiviridae) in populations of bemisia tabaci (hemiptera, aleyrodidae) with differential virus transmission characteristics. | bemisia tabaci (gennadius) is one of the economically most damaging insects to crops in tropical and subtropical regions. severe damage is caused by feeding and more seriously by transmitting viruses. those of the genus begomovirus (geminiviridae) cause the most significant crop diseases and are transmitted by b. tabaci in a persistent circulative mode, a process which is largely unknown. to analyze the translocation and to identify critical determinants for transmission, two populations of b. t ... | 2014 | 25365330 |
| implication of bemisia tabaci heat shock protein 70 in begomovirus-whitefly interactions. | the whitefly bemisia tabaci (gennadius) is a major cosmopolitan pest capable of feeding on hundreds of plant species and transmits several major plant viruses. the most important and widespread viruses vectored by b. tabaci are in the genus begomovirus, an unusual group of plant viruses owing to their small, single-stranded dna genome and geminate particle morphology. b. tabaci transmits begomoviruses in a persistent circulative nonpropagative manner. evidence suggests that the whitefly vector e ... | 2012 | 23015709 |
| diversity and localization of bacterial endosymbionts from whitefly species collected in brazil. | whiteflies (hemiptera: aleyrodidae) are sap-sucking insect pests, and some cause serious damage in agricultural crops by direct feeding and by transmitting plant viruses. whiteflies maintain close associations with bacterial endosymbionts that can significantly influence their biology. all whitefly species harbor a primary endosymbiont, and a diverse array of secondary endosymbionts. in this study, we surveyed 34 whitefly populations collected from the states of sao paulo, bahia, minas gerais an ... | 2014 | 25259930 |
| rapid accumulation and low degradation: key parameters of tomato yellow leaf curl virus persistence in its insect vector bemisia tabaci. | of worldwide economic importance, tomato yellow leaf curl virus (tylcv, begomovirus) is responsible for one of the most devastating plant diseases in warm and temperate regions. the dna begomoviruses (geminiviridae) are transmitted by the whitefly species complex bemisia tabaci. although geminiviruses have long been described as circulative non-propagative viruses, observations such as long persistence of tylcv in b. tabaci raised the question of their possible replication in the vector. we moni ... | 2015 | 26625871 |
| implication of the bacterial endosymbiont rickettsia spp. in interactions of the whitefly bemisia tabaci with tomato yellow leaf curl virus. | numerous animal and plant viruses are transmitted by arthropod vectors in a persistent, circulative manner. tomato yellow leaf curl virus (tylcv) is transmitted by the sweet potato whitefly bemisia tabaci. we report here that infection with rickettsia spp., a facultative endosymbiont of whiteflies, altered tylcv-b. tabaci interactions. a b. tabaci strain infected with rickettsia acquired more tylcv from infected plants, retained the virus longer, and exhibited nearly double the transmission effi ... | 2014 | 24600010 |
| biotype characterization, developmental profiling, insecticide response and binding property of bemisia tabaci chemosensory proteins: role of csp in insect defense. | chemosensory proteins (csps) are believed to play a key role in the chemosensory process in insects. sequencing genomic dna and rna encoding csp1, csp2 and csp3 in the sweet potato whitefly bemisia tabaci showed strong variation between b and q biotypes. analyzing csp-rna levels showed not only biotype, but also age and developmental stage-specific expression. interestingly, applying neonicotinoid thiamethoxam insecticide using twenty-five different dose/time treatments in b and q young adults s ... | 2016 | 27167733 |
| transovarial transmission of rickettsia spp. and organ-specific infection of the whitefly bemisia tabaci. | the whitefly bemisia tabaci is a cosmopolitan insect pest that harbors portiera aleyrodidarum, the primary obligatory symbiotic bacterium, and several facultative secondary symbionts. secondary symbionts in b. tabaci are generally associated with the bacteriome, ensuring their vertical transmission; however, rickettsia is an exception and occupies most of the body cavity, except the bacteriome. the mode of rickettsia transfer between generations and its subcellular localization in insect organs ... | 2012 | 22660706 |
| a peptidoglycan recognition protein acts in whitefly (bemisia tabaci) immunity and involves in begomovirus acquisition. | peptidoglycan recognition proteins (pgrps) are multifunctional pattern recognition proteins. here, we report that a pgrp gene, btpgrp, encodes a pgrp from the whitefly bemisia tabaci (meam1) that binds and kills bacteria in vitro. we analyzed btpgrp transcriptional profiling, and the distribution of the cognate protein within the midgut. fungal infection and wasp parasitization induced expression of btpgrp. silencing btpgrp with artificial media amended with dsrna led to reduced expression of a ... | 2016 | 27892529 |
| deep sequencing-based transcriptome analysis reveals the regulatory mechanism of bemisia tabaci (hemiptera: aleyrodidae) nymph parasitized by encarsia sophia (hymenoptera: aphelinidae). | the whitefly bemisia tabaci is a genetically diverse complex with multiple cryptic species, and some are the most destructive invasive pests of many ornamentals and crops worldwide. encarsia sophia is an autoparasitoid wasp that demonstrated high efficiency as bio-control agent of whiteflies. however, the immune mechanism of b. tabaci parasitization by e. sophia is unknown. in order to investigate immune response of b. tabaci to e. sophia parasitization, the transcriptome of e. sophia parasitize ... | 2016 | 27332546 |
| rnai-mediated mortality of the whitefly through transgenic expression of double-stranded rna homologous to acetylcholinesterase and ecdysone receptor in tobacco plants. | the whitefly bemisia tabaci (genn.) is a pest and vector of plant viruses to crop and ornamental plants worldwide. using rna interference (rnai) to down regulate whitefly genes by expressing their homologous double stranded rnas in plants has great potential for management of whiteflies to reduce plant virus disease spread. using a tobacco rattle virus-derived plasmid for in planta transient expression of double stranded rna (dsrna) homologous to the acetylcholinesterase (ache) and ecdysone rece ... | 2016 | 27929123 |
| analysis of whitefly transcriptional responses to beauveria bassiana infection reveals new insights into insect-fungus interactions. | the fungal pathogen, beauveria bassiana, is an efficient biocontrol agent against a variety of agricultural pests. a thorough understanding of the basic principles of insect-fungus interactions may enable the genetic modification of beauveria bassiana to enhance its virulence. however, the molecular mechanism of insect response to beauveria bassiana infection is poorly understood, let alone the identification of fungal virulent factors involved in pathogenesis. | 2013 | 23861870 |
| transcriptomic and proteomic responses of sweetpotato whitefly, bemisia tabaci, to thiamethoxam. | the sweetpotato whitefly, bemisia tabaci (hemiptera: aleyrodidae), is one of the most widely distributed agricultural pests. although it has developed resistance to many registered insecticides including the neonicotinoid insecticide thiamethoxam, the mechanisms that regulate the resistance are poorly understood. to understand the molecular basis of thiamethoxam resistance, "omics" analyses were carried out to examine differences between resistant and susceptible b. tabaci at both transcriptiona ... | 2013 | 23671574 |
| transcription analysis of neonicotinoid resistance in mediterranean (med) populations of b. tabaci reveal novel cytochrome p450s, but no nachr mutations associated with the phenotype. | bemisia tabaci is one of the most damaging agricultural pests world-wide. although its control is based on insecticides, b. tabaci has developed resistance against almost all classes of insecticides, including neonicotinoids. | 2015 | 26573457 |
| draft genome sequence of rickettsia sp. strain meam1, isolated from the whitefly bemisia tabaci. | we report the draft genome sequence of the rickettsia sp. strain meam1, which is a facultative symbiont from an invasive species of the whitefly bemisia tabaci. the total length of the assembled genome is approximately 1.24 mb, with 335 scaffolds and 1,247 coding sequences predicted within the genome. | 0 | 22887655 |
| competitive ability and fitness differences between two introduced populations of the invasive whitefly bemisia tabaci q in china. | our long-term field survey revealed that the cardinium infection rate in bemisia tabaci q (also known as biotype q) population was low in shandong, china over the past few years. we hypothesize that (1) the cardinium-infected (c+) b. tabaci q population cannot efficiently compete with the cardinium-uninfected (c-) b. tabaci q population; (2) no reproductive isolation may have occurred between c+ and c-; and (3) the c- population has higher fitness than the c+ population. | 2014 | 24945699 |
| bioactivity of indigenous medicinal plants against the cotton whitefly, bemisia tabaci. | forty-one methanol extracts of 28 indigenous medicinal plant species were tested for their insecticidal bioactivity against cotton whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae), adults and second nymphal instars under controlled conditions. this study is within a bioprospection context, in the form of utilizing local plant species as an alternative in sustainable agriculture development. eighteen and nine plant extracts caused a significant decrease in number of live adult and ny ... | 0 | 25204756 |
| small rnas from bemisia tabaci are transferred to solanum lycopersicum phloem during feeding. | the phloem-feeding whitefly bemisia tabaci is a serious pest to a broad range of host plants, including many economically important crops such as tomato. these insects serve as a vector for various devastating plant viruses. it is known that whiteflies are capable of manipulating host-defense responses, potentially mediated by effector molecules in the whitefly saliva. we hypothesized that, beside putative effector proteins, small rnas (srna) are delivered by b. tabaci into the phloem, where the ... | 2016 | 27933079 |
| a primary screening and applying of plant volatiles as repellents to control whitefly bemisia tabaci (gennadius) on tomato. | with the goal of finding a new way to reduce population densities of bemisia tabaci biotype q in greenhouses, seven repellent volatile chemicals and their combinations were screened. the mixture of dlco (d-limonene, citral and olive oil (63:7:30)) had a better cost performance(sc50 = 22.59 mg/ml)to repel whiteflies from settling than the other mixtures or single chemicals. in the greenhouse, in both the choice test and the no-choice tests, the number of adult whiteflies that settled on 1% dlco-t ... | 2016 | 26907368 |
| microrna profiling of the whitefly bemisia tabaci middle east-aisa minor i following the acquisition of tomato yellow leaf curl china virus. | the begomoviruses are the largest and most economically important group of plant viruses exclusively vectored by whitefly (bemisia tabaci) in a circulative, persistent manner. during this process, begomoviruses and whitefly vectors have developed close relationships and complex interactions. however, the molecular mechanisms underlying these interactions remain largely unknown, and the microrna profiles for viruliferous and nonviruliferous whiteflies have not been studied. | 2016 | 26837429 |